Supplementary information for A comparative study of carbon-platinum hybrid nanostructure architecture for amperometric biosensing
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1 Supplementary information for A comparative study of carbon-platinum hybrid nanostructure architecture for amperometric biosensing D.C. Vanegas, M. Taguchi, P. Chaturvedi, S. Burrs, M. Tan, H. Yamaguchi, E.S. McLamore* *To whom correspondence should be addressed. emclamor@ufl.edu; Tel: This file includes: Supplemental Figures -4 Supplemental Tables -
2 A) B) C) D) Supplemental Figure. Representative calibration curves of the CNT-based nanomaterial platforms. Each curve corresponds to one replicate in every treatment of the experimental design. A) Representative calibration curves of the Pt/Ir-MWCNT platforms at different carbon concentrations. B) Representative calibration curves of the Pt/Ir-nPt-MWCNT platforms at different carbon concentrations. C) Representative calibration curves of the Pt/Ir-MWCNT-nPt platforms at different carbon concentrations. D) Representative calibration curves of the Pt/IrnPt-MWCNT-nPt platforms at different carbon concentrations.
3 A) B) C) D) Supplemental Figure. Representative calibration curves of the GO-based nanomaterial platforms. Each curve corresponds to one replicate in every treatment of the experimental design. A) Representative calibration curves of the Pt/Ir-GO platforms at different carbon concentrations. B) Representative calibration curves of the Pt/Ir-nPt-GO platforms at different carbon concentrations. C) Representative calibration curves of the Pt/Ir-RGO-nPt platforms at different carbon concentrations. D) Representative calibration curves of the Pt/Ir-nPt-RGO-nPt platforms at different carbon concentrations.
4 Interaction Plot for Sensitivity Fitted Means nanomaterial configuration nanomaterial configuration Pt/Ir-MWCNT Pt/Ir-MWCNT-nPt Pt/Ir-nPt-MWCNT Pt/Ir-nPt-MWCNT-nPt MWCNT concentration MWCNT concentration Pt/Ir-MWCNT Pt/Ir-MWCNT-nPt Pt/Ir-nPt-MWCNT Pt/Ir-nPt-MWCNT-nPt Supplemental Figure. Interaction plot showing non-combined effect of both factors (configuration/cnt concentration) on the electrochemical sensitivity of the MWCNT-modified electrodes. Supplemental table. Anova general linear model for MWCNT-based nanomaterial platforms. Factor Type Levels Values nanomaterial configuration fixed 4 Pt/Ir-MWCNT, Pt/Ir-MWCNT-nPt, Pt/Ir-nPt-MWCNT, Pt/Ir-nPt-MWCNT-nPt MWCNT concentration fixed,, Analysis of Variance for Sensitivity, using Adjusted SS for Tests Source DF Seq SS Adj SS Adj MS F P nanomaterial configuration MWCNT concentration nanomaterial configuration* MWCNT concentration Error Total S = R-Sq = 76.4% R-Sq(adj) = 65.6% *Normal distribution, independence, and homogeneity of variances were checked through residual plots and Levene s test. 4
5 Interaction Plot for Sensitivity Fitted Means nanomaterial configuration 4 nanomaterial configuration Pt/Ir-GO Pt/Ir-GO-nPt Pt/Ir-nPt-GO Pt/Ir-nPt-GO-nPt 4 GO concentration GO concentration Pt/Ir-GO P t/ir-go -n Pt Pt/Ir-nPt-GO Pt/Ir-nPt-GO-n Pt Supplemental Figure 4. Interaction plot showing the combined effect of both factors (configuration/go concentration) on the electrochemical sensitivity of the GO-modified electrodes. Supplemental table. Anova general linear model for GO-based nanomaterial platforms. Factor Type Levels Values nanomaterial configuration fixed 4 Pt/Ir-GO, Pt/Ir-GO-nPt, Pt/Ir-nPt-GO, Pt/Ir-nPt-GO-nPt GO concentration fixed,, Analysis of Variance for Sensitivity, using Adjusted SS for Tests Source DF Seq SS Adj SS Adj MS F P nanomaterial configuration GO concentration nanomaterial configuration* GO concentration Error Total S =.6844 R-Sq = 94.8% R-Sq(adj) = 9.4% *Normal distribution, independence, and homogeneity of variances were checked through residual plots and Levene s test. 5
6 Supplemental table. Tukey test for GO-based nanomaterial platforms. Grouping Information Using Tukey Method and 95.% Confidence nanomaterial configuration N Mean Grouping Pt/Ir-nPt-GO-nPt A Pt/Ir-GO-nPt 9 8. B Pt/Ir-nPt-GO C Pt/Ir-GO 9.67 D Means that do not share a letter are significantly different. Tukey Simultaneous Tests Response Variable Sensitivity All Pairwise Comparisons among Levels of nanomaterial configuration nanomaterial configuration = Pt/Ir-GO subtracted from: nanomaterial Difference SE of Adjusted configuration of Means Difference T-Value P-Value Pt/Ir-GO-nPt Pt/Ir-nPt-GO Pt/Ir-nPt-GO-nPt nanomaterial configuration = Pt/Ir-GO-nPt subtracted from: nanomaterial Difference SE of Adjusted configuration of Means Difference T-Value P-Value Pt/Ir-nPt-GO Pt/Ir-nPt-GO-nPt nanomaterial configuration = Pt/Ir-nPt-GO subtracted from: nanomaterial Difference SE of Adjusted configuration of Means Difference T-Value P-Value Pt/Ir-nPt-GO-nPt Grouping Information Using Tukey Method and 95.% Confidence GO concentration N Mean Grouping 7.95 A.567 B.5 B Means that do not share a letter are significantly different. Tukey Simultaneous Tests Response Variable Sensitivity All Pairwise Comparisons among Levels of GO concentration GO concentration = subtracted from: GO Difference SE of Adjusted concentration of Means Difference T-Value P-Value GO concentration = subtracted from: GO Difference SE of Adjusted concentration of Means Difference T-Value P-Value
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