Everyone can be an Expert. Electrochemical Detector. Autosampler. Complete HPLC-ECD
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1 Everyone can be an Expert Electrochemical Detector Autosampler Complete HPLC-ECD
2 Eicom ECD-700 Eicom ECD-700 Electrochemical Detector for HPLC Eicom's electrochemical detector, the ECD-700, offers superior stability and sensitivity that reviles that of mass spectrometry detectors, but without the cost and special expertise. Electrochemical detectors easily detect a wide range of easily oxidized or reduced compounds, and you can rely of Eicom's extensive experience to help you find the best set-up for your research goals. Depending of the requirements of a particular method, the ECD-700 can be configured with up to two detector cells. Both amperometric and coulometric types are available. Features High Sensitivity, 30 fg sensitivity for Dopamine at S/N ratio=3. Easy system setup and ready-to-use applications. Instant access to the working electrode of the amperometric detector cell. Expandable design for more demanding users or protocols. ECD-700 Configurations Standard One amperometric detector cell with single graphite working electrode. Optional Configurations Coulometric Cell Standard Option 1 Option 2 Option 3 1 st Detector Cell A da A /da A /da/c 2 nd Detector Cell A C A: Amperometric detector, da: Dual amperometric working electrodes that share a common reference electrode and can be installed in parallel or series with the flow. C: Coulometric flow cell. Amperometric Cell Amperometry vs. Coulometry In amperometry the analyte contacts the surface of a solid working electrode. Amperometry achieves better signal-to-noise ratios resulting in better sensitivity. Coulometry depends on porous working electrode to promote the complete electrolysis of the analyte as it passes. At Eicom, we find the best approach is to employ coulometric cells to prepare the analyte for detection with an amperometric cell. Working Electrode Designs or Amperometry Dual Amperometry Coulometry
3 Eicom ECD-700 Simplicity + Expandability The keyword during the development of the ECD-700 was expandability. Over the past 25 years of Eicom ECD products, our goal has been to help users kick start new projects with our ready-to-use applications and reliable instruments. And now for more demanding applications, we have introduced the ECD-700, which has two detector cell capacity and allows for many combinations of detector types and working electrodes. Heart of Sensitivity, Amperometric Detector Cell Eicom's advanced working electrode design requires much less maintenance than many ECD users may have become accustomed to. When the need does arise, the electrode can easily be accessed to replace it or wipe off any chemical build-up. Even after the cell is opened, the initial baseline signal is quickly restored without worry. Diagram of Detector Cell Working Electrode EICOM WE-XX Gasket GS-XX The pressure inside the cell is maintained by a spring-loaded ball assembly that ensures a constant pressure. The ball compresses the working electrode assembly at one point to evenly distribute the pressure across the working electrode. Variety of Working Electrodes Materials Graphite Electrode WE-3G, very stable and best S/N ratio. Little maintenance is required. Included in the standard configuration. Pure Graphite WE-PG, stable even at higher applied voltage Glassy Carbon WE-GC, conventional working electrode Platinum WE-PT, good for hydrogen peroxide. Commonly used with inline enzyme reactor columns. Gold WE-AU, well suited for thiol group detection Silver WE-AG Dual working electrode for use with Eicom's amperometric detector cell. Graphite WED-3G Glassy Carbon WED-GC Coulometric detector cell working electrode material. Porous Carbon (refer to Option 3 on previous page)
4 Eicom ECD-700 Columns Many Ready-to-use applications Eicom provides complete, step-by-step protocols for a variety of applications. These protocols include instructions for sample collection and preparation, mobile phase recipes, storage and preparation of standards, and exact instrument settings. If you want to modify a method or create your own method, we can advise you on where to start and give you feedback about the data along the way. Typical EC detectable compounds Phenols Anilines Estrogens Opiates Catechols Quinones Hydroxycoumarins Phenothiazines Indoles Tocopherols Purines Thiols Pulse Damper Electrochemical detection is extremely sensitive to changes in pressure. Consequently, each stroke of an HPLC pump can cause oscillations in the baseline signal, which necessitates the use of a pulse damper to obtain the lowest detection limit. If you will not be using an Eicom pump, we recommend using a pulse damper, such as Eicom PL-500. A Pulse damper is not required for Eicom pumps. Specifications of ECD-700 Standard Configuration Detection Method Working Electrode Applied Potential Range Auto Zero Function Reference Electrode Counter Electrode Cell Volume Inlet Tubing OD Input Output Auto Zero Input Log File Amperometric, DC with 3 electrode potentiostat Standard single graphite. Optional dual working electrode with a shared reference electrode. -2,000 mv ~ +2,000 mv in increments of 1mV -1,000 na ~ +1,000 na, resets in less than 0.7 sec. Each detector cell zeros separately. When dual electrode is installed, the signal from both applied voltages zero together. Ag / AgCl Stainless Steel 316 Active area = 1 µl. Outlet has 100 µl reservoir 1/16 inch Contact closure signal, DC 0 V ~ 5 V, ON 1 mv OFF 10 µa Delay of 0 sec, 3 sec and 6 sec after 1sec signal input Accept DC 0 V ~ 5 V, ON 1 mv OFF 10 µa User can save a log of reference and working electrode replacements for each channel through keypad Specification and design of products may change without notice. US Office 7313 Carroll Road Suite F, San Diego, CA Phone Eicom Europe 1A Dartmouth Terrace Ranelagh Dublin 6, Ireland Phone Headquarters 113 Kitaenmendencho Shimotoba Fushimiku Kyoto-Shi Kyoto-Fu Japan Phone
5 Eicom Complete 700 Series Eicom 700 Series Complete HPLC-Electrochemical Detector System If you need the very best performance of Eicom ECD-700, we recommend using the three 700 Series components as one system. The 700 Series includes an HPLC Pump (EP-700), Temperature Controller (ATC-700) and Electrochemical Detector (ECD-700). When used together, we promise the best results. Sensitivity comes from more than just the detector. To achieve optimal performance from electrochemical detectors, there are important considerations. The Eicom 700 Series Total System addresses all of these requirements to ensure the best sensitivity. A chemically inert or, at a minimum, passivated interior because metal ions increase the background signal A means to stabilize the baseline because oscillations in the pressure caused by the HPLC pump produce concomitant oscillations in the signal baseline Effective temperature control because electrochemical detection is very sensitive to temperature Eicom EP-700 HPLC Pump No more pulse dampers required! Revolutionary pump design can effectively quench flow pulses. Built-in, computer control continually adjusts the piston velocity during each stroke to minimize Complete HPLC-ECD fluctuations in the pressure. Pulse free pumping works in both gradient and isocratic modes. Because the EP-700 does not require a damper, the volume between a column and the HPLC pump is minimal. This means rapid acquisition of steady-state pressure after starting the pump and more immediate solvent effects that are important to accurate gradients. An innovative 300 µl volume degasser is built in to guarantee continuous pumping and preventing noise at the detector. Signal Baselines Pulse-free Mode OFF Pulse-free Mode ON Standard Configuration EP-700M --- one micro flow pump (10 to 500 µl /min ) Options EP-700H --- one high flow type pump EP-700MM --- two micro flow pumps with gradient controller EP-700HH --- two high flow pumps with gradient controller EP-700MH --- one micro flow and one high flow pump
6 Eicom Complete 700 Series Eicom ATC-700 Temperature Controller The Eicom ATC-700 uses a Peltier element that both heats and cools by convection to maintain precise temperature control. The system responds to just a 0.05 C change and controls the temperature of the both the columns and ECD cells. Specifications of EP-700 and ATC-700 Pump Unit (EP-700) Type Dual reciprocating piston pump Internal Volume 300 µl/channel Construction Chemically inert materials at all liquid contacting surfaces Maximum Flow Rate 3 ml/min Flow Rate Ranges Liquid Contacting Surfaces Pressure Limit Gradient Mode Micro flow type: 100 to 500 µl/min with 1 µl/min increments, 4 µl/stroke High flow type: 0.5 to 3.00 ml/min with 0.01 ml/min increments, 32 µl/stroke PEEK, Sapphire, Ruby, PTFE, PCTFE 20 MPa High pressure linear gradient, 10 steps. Available when 2 same pump installed. Casing (EP-700) Dimension Weight Power Supply Temperature Controller (ATC-700) Temperature Regulation Setting Range 400 (w) x 400 (d) x 190 (h) mm approx. 13 kg with one pump, approx. 16 kg with two pumps AC V, 50/60 Hz, 200 W Peltier device for heating and cooling by convection 15 to 55 C, 1 C increament External Signal Input: Gradeint Start/Stop, Gradient Initialization Output: Pump Pressure Recommended Range Column Capacity 20 to 45 C at 25 C ambient 25 cm x 2 HPLC columns Degasser Unit (EP-700) Degassing Chamber Volume 300 µl x 2 channels Dimension Weight 400 (w) x 400 (d) x 145 (h) mm approx. 10 kg Lines 2 Channels Power Supply AC V, 50/60 Hz, 210 VA Eicom AS-700 INSIGHT Autosampler We know your time is limited. Eicom's reliable autosampler injects samples while you are away. Autosamplers are must have item for knowledgeable HPLC users. For more details, please see the INSIGHT Autosampler brochure. INSIGHT Specification and design of products may change without notice. US Office 7313 Carroll Road Suite F, San Diego, CA Phone Eicom Europe 1A Dartmouth Terrace Ranelagh Dublin 6, Ireland Phone Headquarters 113 Kitaenmendencho Shimotoba Fushimiku Kyoto-Shi Kyoto-Fu Japan Phone
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