TRAINING COURSE ON MOLECULAR MARKER TECHNIQUES AND FINGERPRINTING AGRICULTURAL GENETIC ENGINEERING RESEARCH INSTITUTE (AGERI)
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1 UNCTAD's Network of Centres of Excellence TRAINING COURSE ON MOLECULAR MARKER TECHNIQUES AND FINGERPRINTING AGRICULTURAL GENETIC ENGINEERING RESEARCH INSTITUTE (AGERI) SUNDAY: GIZA, EGYPT 1 12 JULY 2007 PROGRAMME TIMETABLE 09:00 09:30 Registration 09:30 10:30 Opening session 11:00 12:30 Tour of AGERI 12:30 01:30 Lunch Time 13:30 15:00 Lecture 1 DNA structure and replication 15:00 15:30 Coffee Break 15:30 17:00 Lecture 2 Genome organization and basic strategies for DNA isolation MONDAY: :00 11:30 Practical 1 DNA Extraction (plants) 11:30 12:00 Coffee Break 12:00 13:30 Practical 2 13:30 14:30 Lunch Break 14:30 15:30 Lecture 3 Polymorphic Chain Reaction and its applications Dr. Hala Essia 16:00 17:00 Practical 3 1
2 TUESDAY: :00 10:30 Lecture 4 Molecular markers 11:00 13:30 Practical 4 DNA Extraction (Blood) Dr. Ahmed El-Beltagy 14:30 15:30 Practical 5 16:00 17:00 Practical 6 Preparation of agarose gel and estimation of DNA concentration WEDNESDAY: :00 10:30 Lecture 5 Restriction enzymes and other enzymes used in molecular biology Dr. Ahmed Ashoub 11:00 13:30 Practical 7 Randomly amplified polymorphic DNA technique and preparation of Polymorphic Chain Reactions 14:30 15:30 Lecture 6 Cloning and cloning vectors Dr. Ayman Diab 16:00 17:00 Lecture 7 Cloning and cloning vectors (cont.) Dr. Ayman Diab 2
3 THURSDAY: :00 10:30 Practical 8 Randomly amplified polymorphic DNA technique and preparation of Polymorphic Chain Reactions (cont.) 11:00 13:30 Practical 9 Preparation of agarose gel for Polymorphic Chain Reaction product and evaluation of PCR results 14:30 15:30 Practical 10 Data analysis of Randomly amplified polymorphic DNA results 16:00 17:00 Practical 11 Data analysis of Randomly amplified polymorphic DNA results (cont.) SUNDAY: :00 10:00 Lecture 8 applications Dr. Sami El-Adawy 10:00 10:30 Coffee Break 10:30 13:30 Practical 12 preparation of Polymorphic Chain Reactions 14:30 15:30 Practical 13 Preparation of acrylamide gel for Polymorphic Chain Reaction products (cont.) 16:00 17:00 Practical 14 Preparation of acrylamide gel for Polymorphic Chain Reaction products (cont.) 3
4 MONDAY: :00 11:00 Practical 15 preparation of Polymorphic Chain Reactions (cont.) 11:00 11:30 Coffee Break 11:30 12:00 Practical 16 Amplified Fragment Length Polymorphism run for Polymorphic Chain Reaction product and evaluation of PCR results 12:00-13:30 Lecture 9 Sustainable Agriculture: The 21 st Century Challenge for Biotechnologists 14:30 15:30 Practical 17 Data analysis of Amplified Fragment Length Polymorphism results 16:00 17:00 Practical 18 Data analysis of Amplified Fragment Length Polymorphism results (cont.) TUESDAY: :00 10:30 Practical 19 Simple Sequence Repeats technique and preparation of Polymorphic Chain Reactions 11:00 01:30 Practical 20 Simple Sequence Repeats technique and preparation of Polymorphic Chain Reactions (cont.) 01:30 02:30 Lunch Time 02:30 03:30 Practical 21 Preparation of agarose gel for Polymorphic Chain Reaction products and evaluation of PCR results 03:30 04:00 Coffee Break 04:00 05:00 Practical 22 Data analysis of Simple Sequence Repeats results 4
5 WEDNESDAY: :00 10:30 Wrap-up of molecular markers and discussion 11:00 12:00 Lecture 10 Biosafety Mr. Hesham El-Sheshtawy 12:00 02:00 Lecture 11 The Role of Biotechnology in Delivering Food Security: Opportunities and Constraints 01:30 02:30 Lunch Time 02:30 03:30 Lecture 12 Intellectual property rights in agricultural biotechnology Dr. Ismail Dahish 03:30 04:00 Coffee Break 04:00 05:00 Lecture 12 Intellectual property rights in agricultural biotechnology Dr. Ismail Dahish (cont.) THURSDAY: :00 10:30 Lecture 13 An Evergreen Revolution for Sub-Saharan Africa 11:00 12:30 Closing session and certificate distribution 5
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