Genomic & RNA Profiling Core Facility

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Myrtle Lane
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Baylor College of Medicine Genomic & RNA Profiling Core Facility Services Price List Effective July 2018 PLEASE NOTE: All prices listed are for internal Baylor College of Medicine researchers with

1 Baylor College of Medicine Genomic & RNA Profiling Core Facility Services Price List Effective July 2018 PLEASE NOTE: All prices listed are for internal Baylor College of Medicine researchers with the following exceptions: - Dan L. Duncan Comprehensive Cancer Center members (DLDCCC price list below Baylor price list) and Digestive Disease Center members receive additional subsidies. - External institutions and commercial companies, please contact GARPcore@bcm.edu to inquire about prices. Regular Baylor Pricing Next-Generation Sequencing Services Service Instrument Flowcell Sequencing* Samples per lane Reads Cost Total Cost (Library Prep & Sequencing) Total RNA-Seq ( ng RNA) NextSeq High-Output PE M $708 $5,667 Total RNA-Seq ( ng RNA) NovaSeq S1 PE M $613 $9,189 Limited Input RNA-Seq (< 1 ng RNA) NovaSeq S1 PE M $945 $9,453 Limited Input RNA-Seq (< 1 ng RNA) NovaSeq S2 PE M $873 $17,453 mrna-seq NextSeq Mid-Output PE M $575 $3,452 mrna-seq NextSeq High-Output PE M $511 $8,182

2 mrna-seq NovaSeq S1 PE M $463 $13,904 small RNA-Seq NextSeq Mid-Output PE M $450 $5,844 small RNA-Seq NextSeq High-Output SR M $429 $8,587 ChIP-Seq NextSeq Mid-Output PE M $592 $2,367 ChIP-Seq NextSeq High-Output SR M $324 $5,190 ChIP-Seq NovaSeq S1 PE M $318 $9,535 Amplicon-seq (Nextera) NextSeq Mid-Output SR M $309 $3,088 Human/Mouse Exome NovaSeq S1 PE M (100X) $811 $12,975 Human/Mouse Exome NovaSeq S4 PE M (200X) $719 $68,977 Human/Mouse WGS NovaSeq S4 PE M (30X) $1,303 $6,517 Human/Mouse WGS NovaSeq S4 PE M (30X) $1,258 25,167 User-prepped libraries NextSeq Mid-Output PE M $1,609 $1,609 User-prepped libraries NextSeq High-Output PE M $3,215 $3,215 User-prepped libraries NovaSeq S1 PE B $8,788 $8,788 User-prepped libraries NovaSeq S2 PE B $14,954 $14,954 User-prepped libraries NovaSeq S4 PE B $20,844 $20,844 PLEASE NOTE: - Different multiplicities and sample numbers result in prices other than the ones listed. Please always contact the core to confirm pricing. - Additional factors may necessitate deeper sequencing for any of the applications listed. - To receive pricing information for additional applications including but not limited to whole-genome, bisulfite, amplicon and targeted sequencing, please contact GARPcore@bcm.edu. - * Sequencing chemistry can be selected as paired-end (PE) or single-read (SR).

3 Dan L. Duncan Comprehensive Cancer Center Member Pricing Next-Generation Sequencing Services Service Instrument Flowcell Sequencing* Samples per lane Reads Cost Total Cost (Library Prep & Sequencing) Total RNA-Seq ( ng RNA) NextSeq High-Output PE M $643 $5,144 Total RNA-Seq ( ng RNA) NovaSeq S1 PE M $556 $8,339 Limited Input RNA-Seq (< 1 ng RNA) NovaSeq S1 PE M $858 $8,580 Limited Input RNA-Seq (< 1 ng RNA) NovaSeq S2 PE M $792 $15,843 mrna-seq NextSeq Mid-Output PE M $504 $3,024 mrna-seq NextSeq High-Output PE M $455 $7,278 mrna-seq NovaSeq S1 PE M $411 $12,340 small RNA-Seq NextSeq Mid-Output PE M $382 $4,964 small RNA-Seq NextSeq High-Output SR M $366 $7,310 ChIP-Seq NextSeq Mid-Output PE M $515 $2,061 ChIP-Seq NextSeq High-Output SR M $275 $4,397 ChIP-Seq NovaSeq S1 PE M $273 $8,179 Amplicon-seq (Nextera) NextSeq Mid-Output SR M $265 $2,652 Human/Mouse Exome NovaSeq S1 PE M (100X) $762 $12,188 Human/Mouse Exome NovaSeq S4 PE M (200X) $676 $64,908 Human/Mouse WGS NovaSeq S4 PE M (30X) $1,255 $6,275 Human/Mouse WGS NovaSeq S4 PE M (30X) $1,226 $24,513

4 User-prepped libraries NextSeq Mid-Output PE M $1,448 $1,448 User-prepped libraries NextSeq High-Output PE M $3,054 $3,054 User-prepped libraries NovaSeq S1 PE B $8,620 $8,620 User-prepped libraries NovaSeq S2 PE B $14,786 $14,786 User-prepped libraries NovaSeq S4 PE B $20,676 $20,676 PLEASE NOTE: - Different multiplicities and sample numbers result in prices other than the ones listed. Please always contact the core to confirm pricing. - Additional factors may necessitate deeper sequencing for any of the applications listed. - To receive pricing information for additional applications including but not limited to whole-genome, bisulfite, amplicon and targeted sequencing, please contact GARPcore@bcm.edu. - * Sequencing chemistry can be selected as paired-end (PE) or single-read (SR).

5 Regular Baylor Pricing NanoString Processing Assay Cost + Gene Expression (total RNA) $34 Gene Expression (lysate) $29 Low Input Amplification $40 mirna & mirge $38 CNV $48 + Prices do not include NanoString reagents and master kits. Gene expression reagents and master kits typically range from $80-$275. Custom codeset pricing is available upon request. Please contact GARPcore@bcm.edu for a quote. Sample Quality Control Services Test Type Cost Bioanalyzer RNA Pico chip $20 Bioanalyzer RNA Nano chip $15 Bioanalyzer DNA High Sensitivity chip $24 Bioanalyzer DNA 1000 chip $15 TapeStation Genomic DNA $15 NanoDrop Spectrophotometer $2 Qubit Fluorometer $6 KAPA qpcr (Illumina libraries only) $25 DNA Shearing Instrument Cost Covaris LE220 $17

Single Cell Genomics

Single Cell Genomics Application Cost Platform/Protoc ol Note Single cell 3 mrna-seq cell lysis/rt/library prep $2460/Sample 10X Genomics Chromium 500-10,000 cells/sample Single cell 5 V(D)J mrna-seq cell