LAS-RIIP course Program. Laboratory Animals in Biomedical Research: theory and practice
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1 LAS-RIIP course Program Laboratory Animals in Biomedical Research: theory and practice May 2015 Auditorium of Hellenic Pasteur Institute (Building 8) Athens, Greece
2 1 st Day, Monday 18 May Animal Welfare Regulations- Legislation (Modules 1 and 2) 08:00-08:20 Registration 08:20-08:30 Menelaos Manoussakis, Director of Hellenic Pasteur Institute Welcome 08:30-08:40 Dimitrios Rapakoulias, Association of the Friends of Hellenic Pasteur Institute Welcome 08:40-08:50 Sylva Haralambous, Hellenic Pasteur Institute Welcome 08:50-09:00 Eirini Fragkiadaki, Hellenic Pasteur Institute Opening remarks 09:00-09:40 Lluis Montoliu, CSIC Centro Nacional de Biotecnologia, Spain Understanding the human genome with the help of mice 09:40-10:20 Lida Anestidou, Institute for Laboratory Animal Research, USA International and EU legislation 10:20-10:40 Coffee break 10:40-11:20 Stavroula Tsinorema, Philosophy and Social Studies, University of Crete, Greece Primate Ethics. Duties regarding non-human animals 11:20-12:00 Francois Busquet, Center for Alternatives to Animal Testing, Belgium 3Rs, animal research and paradigm shift in toxicology 12:00-12:40 Ismene Dontas, School of Medicine, University of Athens, Greece Current educational and training requirements in LAS 12:40-13:10 Katerina Marinou, Greek Ministry of Rural Development and Food Applying for license 13:10-14:00 Lunch break 14:00-16:00 Trainees self-presentation 16:00-16:20 Coffee break Practical Session (Module 3.2) 16:20-18:30 Part I: Introduction to Animal Facility, mouse handling Instructors: Eirini Fragkiadaki, Nadia Kavrochorianou, Ioannis Grivas, Hellenic Pasteur Institute and Francina Langa Vives, Institut Pasteur, France Support personnel: Fotis Badounas, Ariadni Karles, Argyro Lappa, Eleni Malaktari, Melina Markogiannaki. 1
3 2 nd Day, Tuesday 19 May Basics in everyday research practice I (Modules 3.1, 4, 5 and 10) 09:00-09:40 Nikos Kostomitsopoulos, Biomedical Research Foundation of the Academy of Athens (BRFAA) Standardization of Laboratory animals: husbandry, transport, enrichment 09:40-10:20 Argyro Zacharioudaki, ELPEN Pharmaceutical Co. Inc., Greece Welfare and experimental procedures (pain assessment, endpoints) 10:20-11:00 Dimitris Vrettakos, Athens University of Economics and Business Design of experimental procedures (sample size, statistics) 11:00-11:30 Eirini Fragkiadaki, Hellenic Pasteur Institute Breeding strategies 11:30-11:50 Coffee break 11:50-12:30 Jean Jaubert, Institut Pasteur, France Cryopreservation strategies 12:30-13:30 Lunch break Practical Session (Module 3.2, mice) 13:30-15:30 Practical part II - restraint techniques, animal and sex identification 15:30-15:50 Coffee break 15:50-18:30 Practical part III- Administration of substances Instructors: Eirini Fragkiadaki, Nadia Kavrochorianou, Ioannis Grivas, Hellenic Pasteur Institute and Francina Langa Vives, Institut Pasteur, France Support personnel: Fotis Badounas, Ariadni Karles, Argyro Lappa, Eleni Malaktari, Melina Markogiannaki. 2
4 3 rd Day, Wednesday 20 May Basics in everyday research practice II (Modules 4, 5, 6.1 and 7) 09:00-09:40 Massimo Foa, IDEXX Laboratories Limited, UK FELASA health screening and tissue sampling 09:40-10:20 Marion Berard, Institut Pasteur, France Clinical Examination and Common Diseases of small rodents 10:20-11:00 Marion Berard, Institut Pasteur, France The impact of microbiota in animal models 11:00-11:20 Coffee break 11:20-12:00 Lucy Whitfield, Royal Veterinary College, UK Recognition of pain, distress, analgesia 12:00-12:40 Lucy Whitfield, Royal Veterinary College, UK Principles of surgery in mice and rats, anesthesia 12:40-13:00 Eirini Fragkiadaki, Hellenic Pasteur Institute Humane euthanasia 13:00-14:00 Lunch break 14:00-16:00 Self study after the completion of Core modules session 17:00-19:00 Cultural event The Acropolis museum guided tour ( 20:00-23:00 Social event Dinner 3
5 4 th Day, Thursday 21 May Animal Models for Human and Animal Diseases I (Task specific) 09:00-09:10 Sylva Haralambous, Hellenic Pasteur Institute Modeling human diseases 09:10-09:50 Franciscus van der Hoeven, Cancer Research Center, Germany Basic principles of transgenesis in mouse 09:50-10:30 Eleni Douni, B.S.R.C. "Alexander Fleming, Agricultural University of Athens, Greece Identification of novel pathogenic targets in mice through Forward Genetics 10:30-11:10 Dimitra Thomaidou, Hellenic Pasteur Institute In vivo imaging 11:10-11:30 Coffee break 11:30-12:05 Florentia Papastefanaki, Hellenic Pasteur Institute Mouse models for neurological disorders: Behavioral assessment 12:05-12:40 Ioannis Grivas, Hellenic Pasteur Institute Experimental models of arthritis 12:40-13:30 Lunch break Practical Session (Modules 3.2 and 8, mice) 13:30-15:00 Practical part IV - Blood sampling 15:00-15:30 Coffee break 15:30-17:30 Practical part V - Necropsy, collection of tissues Instructors: Eirini Fragkiadaki, Nadia Kavrochorianou, Ioannis Grivas, Hellenic Pasteur Institute and Francina Langa Vives, Institut Pasteur, France Support personnel: Fotis Badounas, Ariadni Karles, Argyro Lappa, Eleni Malaktari, Melina Markogiannaki. 4
6 5 th Day, Friday 22 May Animal Models for Human and Animal Diseases II (Task specific) 09:30-10:10 Vivi Miriagou, Hellenic Pasteur Institute Infectious models for the assessment of antimicrobial efficacy 10:10-10:50 Hechmi Louzir, Institut Pasteur, Tunisia Limits of the experimental models of leishmaniosis for vaccine development 10:50-11:10 Coffee break 11:10-11:50 Serban Morosan, University of Pierre and Marie Curie, France Animal models for human hepatitis viruses and malaria study Practical Session (Modules 3.2, rabbits) 11:50-13:00 Practical Part VI Rabbits Instructors: Eirini Fragkiadaki, Ariadni Karles, Argyro Lappa. 13:00-13:30 Course questionnaire, post-course evaluation sheet- End of the course 13:30-14:30 Lunch 5
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