World-leading LC/MS/MS technology for quantitation and identification API LC/MS/MS System
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1 World-leading LC/MS/MS technology for quantitation and identification API 3000 LC/MS/MS System
2 rel Accelerate your drug development cycle The API 3000 LC/MS/MS System leads the field in sensitivity, specificity, and reliability. The Applied Biosystems/MDS SCIEX API 3000 LC/MS/MS System is at the heart of our total solution for quantitation and identification. The system including the API 3000 mass spectrometer, powerful Analyst software, rugged ion sources, and fully integrated LC equipment sets the standard for dependability in the lab. The API 3000 system has the largest installed base of any LC/MS/MS system because of its proven history of superior performance. The API 3000 system offers: Unsurpassed sensitivity innovative ion optics and pumping technology ensure the lowest limits of detection (LOD) and quantitation (LOQ) in even the most difficult matrices. Superior MS/MS performance revolutionary LINAC collision cell technology provides the fastest scan times without compromising sensitivity or mass spectral quality. Greater throughput advanced software applications, including automated methods development, for the routine analysis of hundreds of samples per day. Unmatched ruggedness and reliability guaranteed by our robust ion sources and interface technology. Ease of use advanced acquisition and processing software completely automates analysis.
3 Technology innovations that ability increase performance and productivity API 3000 system ion path with LINAC collision cell Orifice Plate Skimmer IQ1 LINAC Q0 ST Q1 IQ2 Collision Cell ST3 Q3 DF CEM The patented LINAC high-pressure collision cell accelerates ions through the collision quadrupole, providing increased sensitivity at greatly reduced dwell times. Nitrogen Curtain Gas Interface API Ion Source Curtain Gas Flow Differentially Pumped Interface Orifice Plate Vacuum Chamber Skimmer Exclusive technologies The API 3000 system incorporates exclusive technologies that ensure heightened performance and minimal downtime in your lab. The LINAC high-pressure collision cell provides exceptional sensitivity and performance in all modes of MS/MS operation by ensuring maximum transfer of ions to the detector. The proven Curtain Gas interface greatly reduces the need for routine maintenance by protecting the interface region and quadrupole analyzer from contamination. Proven Curtain Gas interface technology ensures maximum uptime and productivity by protecting the mass analyzer from contamination. Rugged ion sources from Applied Biosystems/MDS SCIEX significantly increase the system s productivity and reliability. IonSpray and APCI (atmospheric pressure chemical ionization) sources are available for a wide range of flow rates and mobile phase compositions.
4 Performance that sets the industry standard Powerful Analyst software achieves fast results with ease. Proven success Applied Biosystems/MDS SCIEX has been at the core of advancements in pharmaceutical research since We have used our knowledge and our customers experience to develop systems that increase the throughput and quality of results in DMPK (drug metabolism pharmacokinetics). Our LC/MS solutions also provide informatics interfacing, help to achieve GLP compliance, and fully meet the recommendations of 21 CFR Part 11. The API 3000 LC/MS/MS System delivers sensitivity, specificity, and ruggedness day after day, year after year that are the standard of the pharmaceutical industry An 800 femtogram injection of minoxidil from a proteinprecipitated plasma sample demonstrates the high-sensitivity performance of the API 3000 system. 1.5 Time, min Time, min Even very low levels of analytes can be accurately quantitated due to the excellent sensitivity of the API 3000 system. This 156 femtogram injection of terfenadine in protein-precipitated plasma is easily quantitated.
5 Combining power results and flexibility with ease of use 4 3 Analyte Area / IS Area Relative Area Response 2 1 r 2 = Analyte Concn. / IS Concn Injection Number Calibration curve for 5-fluorouracil demonstrates broad linear dynamic range to ensure the highest confidence in results. Integrated solution The API 3000 system with Analyst software unites performance and ease of use, simplifying every aspect of data acquisition and processing. Analyst software incorporates five core functions configure, tune, acquire, explore, and quantitate that allow both novice and expert users to generate sophisticated analytical results. 300 injections of diphenhydramine from protein-precipitated plasma over a 24-hour period show excellent precision, with a CV of 2.56%. The high degree of robustness engineered into the API 3000 system provides consistently high-quality results. Our total system-based approach sets the benchmark for performance. This superior level of performance, combined with our tools for higher throughput, meet the productivity demands of DMPK labs.
6 produc Solutions for increased throughput Innovations for higher levels of automation. System versatility Applied Biosystems/MDS SCIEX continues to lead the industry in technology innovations. Our API 3000 system combines superior hardware with flexible software applications that allow you to generate more results in less time. Our patented LINAC collision cell significantly increases sensitivity with greatly reduced dwell times, eliminating cross-talk from background material. Using the LINAC collision cell, you can run n-in-one experiments and analyze multiple components simultaneously. The result: measurably increased lab productivity. Application flexibility Analyst software can be expanded with advanced automated applications Automaton, Information Dependent Acquisition (IDA), and Metabolite ID to extend system functionality for early DMPK screening in drug discovery. The Automaton application performs automated, unattended quantitative analysis of in vivo and in vitro samples from drug discovery and development experiments. Automaton streamlines the process by automating method development, acquisition, and data processing of a set of target compounds. B 7-in-1 "7 1" experiment G A E D F C Time, min The fast scan times and elimination of cross-talk afforded by the LINAC collision cell allow for higher throughput n-in-one experiments in which multiple components can be measured in a single LC/MS/MS run.
7 Software applications tivity for accelerated discovery The Information Dependent Acquisition (IDA) application for Analyst software maximizes the information generated in a single LC/MS run. Using IDA with triple quadrupole systems, you can achieve unprecedented selectivity of candidate ion species with precursor ion and neutral loss scan functions. Simple user interfaces allow you to rapidly select specific mass and retention time (SMART) filters and mass inclusion lists, which can further increase the information yield of an LC/MS/MS run. The Metabolite ID software application automates metabolite profiling, saving time and increasing sample throughput. Predefined common metabolite transformations, as well as user-defined transformations, are screened during the acquisition of an initial LC/MS run. A subsequent run is automatically generated to provide LC/MS/MS structural information on the metabolites found in the screen. The API 3000 system using Analyst software also supports integrated control of a variety of LC platforms for greater system flexibility and productivity. Applied Biosystems/MDS SCIEX commitment to ongoing product development is demonstrated through our constant innovation of LC/MS technology.
8 from our lab to yours Applied Biosystems/MDS SCIEX introduced the first dedicated LC/MS/MS system based on innovative IonSpray technology in Today, we maintain the largest installed base of dedicated LC/MS/MS systems in the world. Applied Biosystems/MDS SCIEX systems have revolutionized mass spectrometry by dramatically increasing the range and ease with which compounds can be analyzed. The API 3000 LC/MS/MS System is part of a comprehensive line of superior technology solutions for LC/MS and LC/MS/MS. API QSTAR Pulsar Hybrid LC/MS/MS System API 2000 LC/MS/MS System Worldwide Sales Offices Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches into 150 countries on six continents. For international office locations, please call the division headquarters or refer to our Web site at API 150EX LC/MS System Applera Corporation is committed to providing the world s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Applied Biosystems/MDS SCIEX is a joint venture between Applera Corporation and MDS Inc. Headquarters 850 Lincoln Centre Drive Foster City, CA USA Phone: Toll Free: Fax: Mariner API-TOF Workstation For Research Use Only. Not for use in diagnostic procedures. AB (design), Analyst, API 150EX, API 2000, API 3000, Curtain Gas, Mariner, and QSTAR are trademarks, and Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and certain other countries. LINAC, MDS, and SCIEX are trademarks of MDS Inc Applied Biosystems. All rights reserved. Printed in the USA. 3/2001, PJA. Publication 114BR
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