MATERIAL SAFETY DATA SHEET

Transcription

1 MATERIAL SAFETY DATA SHEET Issue Date: May 22, 2006 Revision Date: May 22, 2006 IDENTIFICATION CATALOG NO. SureLINK TM AP Conjugation Kit Reagents that Contain Hazardous Materials Catalog. SureLINK TM Modified AP AP Modification Buffer SFB AP Conjugation Buffer (5X) AP Storage Buffer See Attached Material Data Safety Sheets 910 Clopper Road Gaithersburg, Maryland USA FAX

2 Material Safety Data Sheet NFPA Personal protective equipment Transport Symbol t regulated. Preparation Date Revision Date 22-May-2006 Revision Number 2 1. PRODUCT AND COMPANY IDENTIFICATION Product name SureLINK Modified AP Product code , Chemical Name SANH Modified Alkaline Phosphatase Common Name Recommended use Contact manufacturer Supplier KPL KPL 910 Clopper Road 910 Clopper Road Gaithersburg, Maryland Gaithersburg, Maryland USA USA FAX FAX Emergency telephone number ; HAZARDS IDENTIFICATION Emergency Overview Irritant. Toxic. Physical state Solid Odor amine-like. Potential health effects Principle Routes of Exposure Acute effects Eyes Skin Inhalation Ingestion Chronic effects Moderately irritating to the eyes. Irritating to skin. Harmful in contact with skin. May cause irritation of respiratory tract Toxic if swallowed. Prolonged skin contact may cause skin irritation and/or dermatitis. See Section 11 for additional Toxicological information. Page 1 / 7

3 , SureLINK Modified AP Revision Date 22-May-2006 Main symptoms Redness. Symptoms of overexposure are dizziness, headache, tiredness, nausea, unconsciousness, cessation of breathing. Aggravated Medical Conditions Interactions with other chemicals Potential environmental effects Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. 3. COMPOSITION/INFORMATION ON INGREDIENTS Hazardous components CHEMICAL NAME CAS. WEIGHT % Sodium azide Sodium chloride Tris(hydroxymethyl)aminomethane FIRST AID MEASURES General advice Eye contact Skin contact Inhalation Ingestion tes to physician Show this safety data sheet to the doctor in attendance. In the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Move to fresh air. Artificial respiration and/or oxygen may be necessary. Immediate medical attention is required. Drink plenty of water. Do not induce vomiting without medical advice. Consult a physician if necessary. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Unsuitable extinguishing media Specific hazards arising from the chemical In the event of fire and/or explosion do not breathe fumes. Protective equipment and precautions for firefighters Wear self-contained breathing apparatus and protective suit. Page 2 / 7

4 , SureLINK Modified AP Revision Date 22-May-2006 NFPA Health 2 Flammability 0 Instability 0 6. ACCIDENTAL RELEASE MEASURES Personal precautions Environmental precautions Methods for containment Methods for clean-up Other information Avoid contact with skin, eyes and clothing. Prevent product from entering drains. Prevent further leakage or spillage if safe to do so. Avoid dust formation. Do not breathe dust. Wet with water. Avoid dust formation. Take up mechanically and collect in suitable container for disposal. Clean contaminated surface thoroughly. t applicable 7. HANDLING AND STORAGE Handling Storage Avoid contact with skin, eyes and clothing. Ensure adequate ventilation. Keep containers tightly closed in a cool, well-ventilated place. 8. EXPOSURE CONTROLS / PERSONAL PROTECTION Exposure guidelines Chemical Name ACGIH TLV OSHA PEL Sodium azide Ceiling: 0.11 ppm 0.1 ppm (vacated) Ceiling: 0.29 mg/m³ Tris(hydroxymethyl)aminomethane ne ne Engineering controls Personal protective equipment Eye/face protection Skin protection Respiratory protection Ensure adequate ventilation, especially in confined areas Safety glasses with side-shields. Long sleeved clothing. Protective gloves. In case of insufficient ventilation wear suitable respiratory equipment. Respirator must be worn if exposed to dust. Breathing apparatus with filter. General hygiene considerations Handle in accordance with good industrial hygiene and safety practice. Page 3 / 7

5 , SureLINK Modified AP Revision Date 22-May PHYSICAL AND CHEMICAL PROPERTIES Appearance Odor amine-like. Odor threshold Physical state Solid. ph Flash point Autoignition temperature Flammability Limits in Air Upper Lower Explosive properties Oxidizing properties Evaporation Rate Vapor pressure Vapor density Specific Gravity Solubility Water solubility slightly soluble Partition coefficient (noctanol/water). Viscosity 10. STABILITY AND REACTIVITY Chemical stability Conditions to avoid Incompatible materials Hazardous decomposition products Possibility of hazardous reactions Stable under normal conditions. Temperatures above 60 C. Incompatible with strong acids and bases. Carbon oxides. Nitrogen oxides (NOx). ne under normal processing 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information Toxic if swallowed. Irritant. Component Information Chemical Name LD50 Oral LD50 Dermal LC50 Inhalation Sodium azide 27 mg/kg Mouse 20 mg/kg Rabbit 27 mg/kg Rat Sodium chloride 3 g/kg Rat 10 g/kg Rabbit 42 g/m 3 Rat 1 h 4 g/kg Mouse Tris(hydroxymethyl)aminometha ne 5900 mg/kg Rat Chronic toxicity Carcinogenicity There are no known carcinogenic chemicals in this product. Page 4 / 7

6 , SureLINK Modified AP Revision Date 22-May-2006 Legend: Irritation Corrosivity Sensitization Neurological effects Mutagenic effects Reproductive effects Developmental effects Target Organ effects Irritating to skin. Irritating to eyes. May cause irritation of respiratory tract. Did not show mutagenic effects in animal experiments. 12. ECOLOGICAL INFORMATION Ecotoxicity Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. Persistence/Degradability Bioaccumulation/ Accumulation Mobility in Environmental Media Does not bioaccumulate. 13. DISPOSAL CONSIDERATIONS Waste Disposal Method Contaminated packaging US EPA Waste Number Dispose of in accordance with National, State and Local regulations Empty containers should be taken for local recycling, recovery or waste disposal 14. TRANSPORT INFORMATION DOT t regulated. 15. REGULATORY INFORMATION Page 5 / 7

7 , SureLINK Modified AP Revision Date 22-May-2006 International Inventories Chemical Name TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS Sodium azide X X - X - X X X X X Sodium chloride X X - X - X X X X X Tris(hydroxymethyl)aminomethane X X - X - X X X X X TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS USA Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product contains no chemical or chemicals which are subject to the reporting requirements of the Act and and Title 40n of the Code of Federal Regulations, Part 372. Sodium azide (CAS #: ) SARA 311/312 Hazardous Categorization Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard Yes Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61) ne State Regulations California Proposition 65 This product contains the following Proposition 65 chemicals: ne State Right-to-Know Chemical Name Massachusetts New Jersey Pennsylvania Illinois Rhode Island Sodium azide X X X X Legend: X - Listed Canada WHMIS hazard class D1B Toxic materials Page 6 / 7

8 , SureLINK Modified AP Revision Date 22-May OTHER INFORMATION Prepared By Revision 2 Date Revision summary KPL 910 Clopper Road Gaithersburg, MD May-2006 Product name change. Revision summary Revision 1 Date Product Stewardship 23 British American Blvd. Latham, NY Jul-2005 Disclaimer The information provided on this MSDS is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other material or in any process, unless specified in the text. End of MSDS Page 7 / 7

9 Material Safety Data Sheet NFPA Personal protective equipment Transport Symbol t regulated. Preparation Date Revision Date 23-Jul-2005 Revision Number 2 1. PRODUCT AND COMPANY IDENTIFICATION Product name AP Modification Buffer Product code Chemical Name AP Modification Buffer Common Name Recommended use Contact manufacturer Supplier KPL KPL 910 Clopper Road 910 Clopper Road Gaithersburg, Maryland Gaithersburg, Maryland USA USA FAX FAX Emergency telephone number ; HAZARDS IDENTIFICATION Emergency Overview Irritating to eyes. Physical state Liquid Odor ne. Potential health effects Principle Routes of Exposure Acute effects Eyes Skin Inhalation Ingestion Chronic effects Moderately irritating to the eyes. May cause skin irritation and/or dermatitis. t hazardous by inhalation. May be harmful if swallowed. Prolonged skin contact may cause skin irritation and/or dermatitis. See Section 11 for additional Toxicological information. Page 1 / 7

10 AP Modification Buffer Revision Date 23-Jul-2005 Main symptoms Redness. Aggravated Medical Conditions Interactions with other chemicals Potential environmental effects There is no known ecological information for this product 3. COMPOSITION/INFORMATION ON INGREDIENTS Hazardous components CHEMICAL NAME CAS. WEIGHT % Sodium chloride FIRST AID MEASURES General advice Eye contact Skin contact Inhalation Ingestion tes to physician Show this safety data sheet to the doctor in attendance. In the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Immediate medical attention is not required. Drink plenty of water. Do not induce vomiting without medical advice. Consult a physician if necessary. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Unsuitable extinguishing media Specific hazards arising from the chemical In the event of fire and/or explosion do not breathe fumes. Protective equipment and precautions for firefighters Wear self-contained breathing apparatus and protective suit. NFPA Health 1 Flammability 0 Instability 0 6. ACCIDENTAL RELEASE MEASURES Personal precautions Avoid contact with skin, eyes and clothing. Page 2 / 7

11 AP Modification Buffer Revision Date 23-Jul-2005 Environmental precautions Methods for containment Methods for clean-up Other information Prevent product from entering drains. Prevent further leakage or spillage if safe to do so. Contain or absorb leaking liquid with sand or earth or other suitable substance. Soak up with inert absorbent material. Clean contaminated surface thoroughly. t applicable 7. HANDLING AND STORAGE Handling Storage Avoid contact with skin, eyes and clothing. Ensure adequate ventilation. Keep containers tightly closed in a cool, well-ventilated place. Exposure guidelines 8. EXPOSURE CONTROLS / PERSONAL PROTECTION Engineering controls Personal protective equipment Eye/face protection Skin protection Respiratory protection Ensure adequate ventilation, especially in confined areas Safety glasses with side-shields. Long sleeved clothing. Protective gloves. special protective equipment required. In case of mist, spray or aerosol exposure wear suitable personal respiratory protection and protective suit. General hygiene considerations Handle in accordance with good industrial hygiene and safety practice. Page 3 / 7

12 AP Modification Buffer Revision Date 23-Jul PHYSICAL AND CHEMICAL PROPERTIES Appearance Odor ne. Odor threshold Physical state Liquid. ph 7.4 Flash point Autoignition temperature Boiling point/range ( F) VALUE ~212 Boiling point/range ( C) VALUE ~100 Melting point/range ( F) VALUE ~32 Melting point ( C) VALUE ~0 Flammability Limits in Air Upper Lower Explosive properties Oxidizing properties Evaporation Rate Vapor pressure Vapor density Specific Gravity ~1.0 Density ~1.0 Bulk density ~1.0 Solubility Water solubility Miscible with water Partition coefficient (noctanol/water). Viscosity 10. STABILITY AND REACTIVITY Chemical stability Conditions to avoid Incompatible materials Hazardous decomposition products Possibility of hazardous reactions Stable under normal conditions. Temperatures above 60 C. Incompatible with strong acids and bases. Carbon oxides. Hydrogen chloride gas. oxides of phosphorus. ne under normal processing 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information Irritating to eyes. Component Information Chemical Name LD50 Oral LD50 Dermal LC50 Inhalation Sodium chloride 3 g/kg Rat 4 g/kg Mouse 10 g/kg Rabbit 42 g/m 3 Rat 1 h Page 4 / 7

13 AP Modification Buffer Revision Date 23-Jul-2005 Chronic toxicity Carcinogenicity There are no known carcinogenic chemicals in this product. Legend: Irritation Corrosivity Sensitization Neurological effects Mutagenic effects Reproductive effects Developmental effects Target Organ effects Irritating to eyes. Did not show mutagenic effects in animal experiments. ne. 12. ECOLOGICAL INFORMATION Ecotoxicity Contains no substances known to be hazardous to the environment or not degradable in waste water treatment plants. Persistence/Degradability Bioaccumulation/ Accumulation Mobility in Environmental Media Inherently biodegradable. Does not bioaccumulate. Soluble. 13. DISPOSAL CONSIDERATIONS Waste Disposal Method Contaminated packaging US EPA Waste Number Dispose of in accordance with National, State and Local regulations Empty containers should be taken for local recycling, recovery or waste disposal 14. TRANSPORT INFORMATION DOT t regulated. Page 5 / 7

14 AP Modification Buffer Revision Date 23-Jul REGULATORY INFORMATION International Inventories Chemical Name TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS Sodium chloride X X - X - X X X X X TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS USA Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product contains no chemical or chemicals which are subject to the reporting requirements of the Act and and Title 40n of the Code of Federal Regulations, Part 372. SARA 311/312 Hazardous Categorization Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard Yes Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61) ne State Regulations California Proposition 65 ne State Right-to-Know Legend: X - Listed Canada WHMIS hazard class n-controlled 16. OTHER INFORMATION Page 6 / 7

15 AP Modification Buffer Revision Date 23-Jul-2005 Prepared By Revision Date Revision summary Product Stewardship 23 British American Blvd. Latham, NY Jul-2005 Disclaimer The information provided on this MSDS is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other material or in any process, unless specified in the text. End of MSDS Page 7 / 7

16 Material Safety Data Sheet NFPA Personal protective equipment Transport Symbol t regulated. Preparation Date Revision Date 23-Jul-2005 Revision Number 1 1. PRODUCT AND COMPANY IDENTIFICATION Product name SFB Product code , , , Chemical Name Succinimidyl-4-Formyl Benzoate Common Name Recommended use Contact manufacturer KPL 910 Clopper Road Gaithersburg, Maryland USA FAX Supplier KPL 910 Clopper Road Gaithersburg, Maryland USA FAX Emergency telephone number ; HAZARDS IDENTIFICATION Emergency Overview This product has not been thoroughly investigated however, similar compounds are know to be chemically reactive with proteins and other biochemicals and should be treated as potentially hazardous. Harmful in contact with skin. Harmful if swallowed. Harmful by inhalation. Physical state Solid Powder Odor Slight. Potential health effects Principle Routes of Exposure Acute effects Eyes Skin Inhalation Ingestion Chronic effects Eye contact, Skin contact, Inhalation, Ingestion. May cause eye irritation with susceptible persons. Harmful in contact with skin. Harmful by inhalation. Harmful if swallowed. Page 1 / 7

17 , , , SFB Revision Date 23-Jul-2005 See Section 11 for additional Toxicological information. Main symptoms Aggravated Medical Conditions Skin disorders. Interactions with other chemicals Potential environmental effects There is no known ecological information for this product. 3. COMPOSITION/INFORMATION ON INGREDIENTS Hazardous components CHEMICAL NAME CAS-. WEIGHT % Succinimidyl-4-Formyl Benzoate CAS# FIRST AID MEASURES General advice Eye contact Skin contact Inhalation Ingestion tes to physician Show this safety data sheet to the doctor in attendance. If symptoms persist, call a physician. In the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Consult a physician. Move to fresh air. If breathing is difficult, give oxygen. Immediate medical attention is required. Drink plenty of water. Do not induce vomiting without medical advice. Immediate medical attention is required. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Suitable extinguishing media Powdered material may form explosive dust-air mixtures. Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Use:. Water. Carbon dioxide (CO2). alcohol-resistant foam. dry chemical. Unsuitable extinguishing media Specific hazards arising from the chemical In the event of fire and/or explosion do not breathe fumes. Protective equipment and precautions for firefighters Wear self-contained breathing apparatus and protective suit. NFPA Health 2 Flammability 1 Instability 0 Page 2 / 7

18 , , , SFB Revision Date 23-Jul ACCIDENTAL RELEASE MEASURES Personal precautions Environmental precautions Methods for containment Methods for clean-up Other information Ensure adequate ventilation. Avoid dust formation. Take precautionary measures against static discharges. Remove all sources of ignition. Should not be released into the environment. Take up mechanically and collect in suitable container for disposal. Avoid dust formation. t applicable 7. HANDLING AND STORAGE Handling Storage Handle in accordance with good industrial hygiene and safety practice. Avoid dust formation in confined areas. Fine dust dispersed in air may ignite. Take precautionary measures against static discharges. Remove all sources of ignition. Ensure adequate ventilation. Keep container tightly closed in a dry and well-ventilated place. Keep away from heat. Keep in properly labelled containers. Exposure guidelines 8. EXPOSURE CONTROLS / PERSONAL PROTECTION Engineering controls Personal protective equipment Eye/face protection Skin protection Respiratory protection Ensure adequate ventilation, especially in confined areas Tightly fitting safety goggles. Long sleeved clothing. Protective gloves. Respirator must be worn if exposed to dust. Breathing apparatus with filter. General hygiene considerations Use only in an area equipped with a safety shower. Page 3 / 7

19 , , , SFB Revision Date 23-Jul PHYSICAL AND CHEMICAL PROPERTIES Appearance white. Odor Slight. Odor threshold Physical state Solid. Powder. ph Flash point Autoignition temperature Flammability Limits in Air Upper Lower Explosive properties Oxidizing properties Evaporation Rate Vapor pressure Vapor density Specific Gravity Density Bulk density Solubility Water solubility slightly soluble Partition coefficient (noctanol/water). Viscosity Molecular Weight STABILITY AND REACTIVITY Chemical stability Conditions to avoid Incompatible materials Hazardous decomposition products Possibility of hazardous reactions Stable under normal conditions. Avoid dust formation. Keep away from open flames, hot surfaces and sources of ignition. Strong oxidizing agents. Carbon oxides. Nitrogen oxides (NOx). ne under normal processing 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information Harmful in contact with skin. Harmful if swallowed. Harmful by inhalation. Component Information Chronic toxicity Carcinogenicity There are no known carcinogenic chemicals in this product. Legend: Irritation Page 4 / 7

20 , , , SFB Revision Date 23-Jul-2005 Corrosivity Sensitization Neurological effects Mutagenic effects Reproductive effects Developmental effects Target Organ effects Other adverse effects 12. ECOLOGICAL INFORMATION Ecotoxicity Persistence/Degradability Bioaccumulation/ Accumulation Mobility in Environmental Media 13. DISPOSAL CONSIDERATIONS Waste Disposal Method Contaminated packaging US EPA Waste Number Dispose of in accordance with National, State and Local regulations Empty containers should be taken for local recycling, recovery or waste disposal 14. TRANSPORT INFORMATION DOT t regulated. 15. REGULATORY INFORMATION International Inventories Page 5 / 7

21 , , , SFB Revision Date 23-Jul-2005 Chemical Name TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS Succinimidyl-4-Formyl Benzoate TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS USA Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product contains no chemical or chemicals which are subject to the reporting requirements of the Act and and Title 40n of the Code of Federal Regulations, Part 372. SARA 311/312 Hazardous Categorization Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard Yes Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61) ne State Regulations California Proposition 65 This product contains the following Proposition 65 chemicals: State Right-to-Know Legend: X - Listed Canada WHMIS hazard class n-controlled 16. OTHER INFORMATION Prepared By Revision Date Product Stewardship 23 British American Blvd. Latham, NY Jul-2005 Page 6 / 7

22 , , , SFB Revision Date 23-Jul-2005 Revision summary Disclaimer The information provided on this MSDS is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other material or in any process, unless specified in the text. End of MSDS Page 7 / 7

23 Material Safety Data Sheet NFPA Personal protective equipment Transport Symbol t regulated. Preparation Date Revision Date 23-Jul-2005 Revision Number 2 1. PRODUCT AND COMPANY IDENTIFICATION Product name AP Conjugation Buffer (5X) Product code Chemical Name AP Conjugation Buffer (5X) Common Name Recommended use Contact manufacturer Supplier KPL KPL 910 Clopper Road 910 Clopper Road Gaithersburg, Maryland Gaithersburg, Maryland USA USA FAX FAX Emergency telephone number ; HAZARDS IDENTIFICATION Emergency Overview Irritating to eyes. Physical state Liquid Odor ne. Potential health effects Principle Routes of Exposure Acute effects Eyes Skin Inhalation Ingestion Chronic effects Moderately irritating to the eyes. May cause skin irritation and/or dermatitis. t hazardous by inhalation. May be harmful if swallowed. Prolonged skin contact may cause skin irritation and/or dermatitis. See Section 11 for additional Toxicological information. Page 1 / 7

24 AP Conjugation Buffer (5X) Revision Date 23-Jul-2005 Main symptoms Redness. Aggravated Medical Conditions Interactions with other chemicals Potential environmental effects There is no known ecological information for this product 3. COMPOSITION/INFORMATION ON INGREDIENTS Hazardous components CHEMICAL NAME CAS. WEIGHT % Sodium chloride Citric acid FIRST AID MEASURES General advice Eye contact Skin contact Inhalation Ingestion tes to physician Show this safety data sheet to the doctor in attendance. In the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Immediate medical attention is not required. Drink plenty of water. Do not induce vomiting without medical advice. Consult a physician if necessary. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Unsuitable extinguishing media Specific hazards arising from the chemical In the event of fire and/or explosion do not breathe fumes. Protective equipment and precautions for firefighters Wear self-contained breathing apparatus and protective suit. NFPA Health 1 Flammability 0 Instability 0 6. ACCIDENTAL RELEASE MEASURES Personal precautions Avoid contact with skin, eyes and clothing. Page 2 / 7

25 AP Conjugation Buffer (5X) Revision Date 23-Jul-2005 Environmental precautions Methods for containment Methods for clean-up Other information Prevent product from entering drains. Prevent further leakage or spillage if safe to do so. Contain or absorb leaking liquid with sand or earth or other suitable substance. Soak up with inert absorbent material. Clean contaminated surface thoroughly. t applicable 7. HANDLING AND STORAGE Handling Storage Avoid contact with skin, eyes and clothing. Ensure adequate ventilation. Keep containers tightly closed in a cool, well-ventilated place. Exposure guidelines 8. EXPOSURE CONTROLS / PERSONAL PROTECTION Engineering controls Personal protective equipment Eye/face protection Skin protection Respiratory protection Ensure adequate ventilation, especially in confined areas Safety glasses with side-shields. Long sleeved clothing. Protective gloves. special protective equipment required. In case of mist, spray or aerosol exposure wear suitable personal respiratory protection and protective suit. General hygiene considerations Handle in accordance with good industrial hygiene and safety practice. Page 3 / 7

26 AP Conjugation Buffer (5X) Revision Date 23-Jul PHYSICAL AND CHEMICAL PROPERTIES Appearance Odor ne. Odor threshold Physical state Liquid. ph determined. Flash point Autoignition temperature Boiling point/range ( F) VALUE ~212 Boiling point/range ( C) VALUE ~100 Melting point/range ( F) VALUE ~32 Melting point ( C) VALUE ~0 Flammability Limits in Air Upper Lower Explosive properties Oxidizing properties Evaporation Rate Vapor pressure Vapor density Specific Gravity ~1.0 Density ~1.0 Bulk density ~1.0 Solubility Water solubility Miscible with water Partition coefficient (noctanol/water). Viscosity 10. STABILITY AND REACTIVITY Chemical stability Conditions to avoid Incompatible materials Hazardous decomposition products Possibility of hazardous reactions Stable under normal conditions. Temperatures above 60 C. Incompatible with strong acids and bases. Carbon oxides. Hydrogen chloride gas. ne under normal processing 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information Irritating to eyes. Component Information Chemical Name LD50 Oral LD50 Dermal LC50 Inhalation Sodium chloride 3 g/kg Rat 10 g/kg Rabbit 42 g/m 3 Rat 1 h 4 g/kg Mouse Citric acid 3 g/kg Rat 5040 mg/kg Mouse data data Page 4 / 7

27 AP Conjugation Buffer (5X) Revision Date 23-Jul-2005 Chronic toxicity Carcinogenicity There are no known carcinogenic chemicals in this product. Legend: Irritation Corrosivity Sensitization Neurological effects Mutagenic effects Reproductive effects Developmental effects Target Organ effects Irritating to eyes. Did not show mutagenic effects in animal experiments. ne. 12. ECOLOGICAL INFORMATION Ecotoxicity Contains no substances known to be hazardous to the environment or not degradable in waste water treatment plants. Citric acid Microtox Data Photobacterium phosphoreum EC50=14 mg/l (15 min) Persistence/Degradability Bioaccumulation/ Accumulation Mobility in Environmental Media Inherently biodegradable. Does not bioaccumulate. Soluble. Citric acid log Pow = DISPOSAL CONSIDERATIONS Waste Disposal Method Contaminated packaging US EPA Waste Number Dispose of in accordance with National, State and Local regulations Empty containers should be taken for local recycling, recovery or waste disposal 14. TRANSPORT INFORMATION Page 5 / 7

28 AP Conjugation Buffer (5X) Revision Date 23-Jul-2005 DOT t regulated. 15. REGULATORY INFORMATION International Inventories Chemical Name TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS Sodium chloride X X - X - X X X X X Citric acid X X - X - X X X X X TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS USA Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product contains no chemical or chemicals which are subject to the reporting requirements of the Act and and Title 40n of the Code of Federal Regulations, Part 372. SARA 311/312 Hazardous Categorization Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard Yes Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61) ne State Regulations California Proposition 65 ne State Right-to-Know Page 6 / 7

29 AP Conjugation Buffer (5X) Revision Date 23-Jul-2005 Legend: X - Listed Canada WHMIS hazard class n-controlled 16. OTHER INFORMATION Prepared By Revision Date Revision summary Product Stewardship 23 British American Blvd. Latham, NY Jul-2005 Disclaimer The information provided on this MSDS is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other material or in any process, unless specified in the text. End of MSDS Page 7 / 7

30 Material Safety Data Sheet NFPA Personal protective equipment Transport Symbol t regulated. Preparation Date 07/08/2005 Revision Date 23-Jul-2005 Revision Number 1 1. PRODUCT AND COMPANY IDENTIFICATION Product name AP Storage Buffer Product code Chemical Name AP Storage Buffer Common Name Recommended use Contact manufacturer Supplier KPL KPL 910 Clopper Road 910 Clopper Road Gaithersburg, Maryland Gaithersburg, Maryland USA USA FAX FAX Emergency telephone number ; HAZARDS IDENTIFICATION Emergency Overview May cause irritation of respiratory tract. Irritating to eyes. May cause skin irritation and/or dermatitis. Physical state Liquid Odor Slight. Potential health effects Principle Routes of Exposure Acute effects Eyes Skin Inhalation Ingestion Chronic effects Eye contact, Skin contact, Inhalation. May cause dehydration. Moderately irritating to the eyes. May cause skin irritation in susceptible persons. May cause irritation of respiratory tract. Inhalation of high vapour concentrations can cause CNS-depression and narcosis. May be harmful if swallowed. Prolonged skin contact may cause skin irritation and/or dermatitis. See Section 11 for additional Toxicological information. Page 1 / 7

31 AP Storage Buffer Revision Date 23-Jul-2005 Aggravated Medical Conditions Skin disorders. Interactions with other chemicals Potential environmental effects There is no known ecological information for this product 3. COMPOSITION/INFORMATION ON INGREDIENTS Hazardous components CHEMICAL NAME CAS. WEIGHT % Glycerol FIRST AID MEASURES General advice Eye contact Skin contact Inhalation Ingestion tes to physician Show this safety data sheet to the doctor in attendance. In the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Move to fresh air in case of accidental inhalation of vapours. If symptoms persist, call a physician. Drink plenty of water. Do not induce vomiting without medical advice. Consult a physician if necessary. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Unsuitable extinguishing media ne. Specific hazards arising from the chemical In the event of fire and/or explosion do not breathe fumes. Protective equipment and precautions for firefighters Wear self-contained breathing apparatus and protective suit. NFPA Health 1 Flammability 0 Instability 0 6. ACCIDENTAL RELEASE MEASURES Personal precautions Environmental precautions Avoid contact with skin, eyes and clothing. Prevent further leakage or spillage if safe to do so. Page 2 / 7

32 AP Storage Buffer Revision Date 23-Jul-2005 Methods for containment Methods for clean-up Other information Contain or absorb leaking liquid with sand or earth or other suitable substance. Soak up with inert absorbent material. Clean contaminated surface thoroughly. 7. HANDLING AND STORAGE Handling Storage Keep in a well-ventilated place. In case of insufficient ventilation, wear suitable respiratory equipment. Wear personal protective equipment. Keep container tightly closed in a dry and well-ventilated place. Exposure guidelines 8. EXPOSURE CONTROLS / PERSONAL PROTECTION Chemical Name ACGIH TLV OSHA PEL Glycerol TWA: 10 mg/m³ TWA: 5 mg/m³ TWA: 15 mg/m³ Engineering controls Personal protective equipment Eye/face protection Skin protection Respiratory protection Ensure adequate ventilation, especially in confined areas. Safety glasses with side-shields. Long sleeved clothing. Protective gloves. special protective equipment required. In case of mist, spray or aerosol exposure wear suitable personal respiratory protection and protective suit. General hygiene considerations Handle in accordance with good industrial hygiene and safety practice. Page 3 / 7

33 AP Storage Buffer Revision Date 23-Jul PHYSICAL AND CHEMICAL PROPERTIES Appearance Clear. Odor Slight. Odor threshold t available. Physical state Liquid. ph 7 Flash point Autoignition temperature Boiling point/range ( F) VALUE > 212 Boiling point/range ( C) VALUE > 100 Melting point/range Melting point/range ( F) VALUE > 32 Melting point ( C) VALUE > 0 Flammability Limits in Air Upper ne Lower ne Explosive properties Oxidizing properties ne Evaporation Rate Vapor pressure Vapor density >1 Specific Gravity ~1.2 Density ~1.2 Bulk density ~1.2 Solubility completely miscible Water solubility Miscible with water Partition coefficient (noctanol/water). Viscosity Oxidizing properties ne 10. STABILITY AND REACTIVITY Chemical stability Conditions to avoid Incompatible materials Hazardous decomposition products Possibility of hazardous reactions Stable under normal conditions. Temperatures above 60 C. Strong oxidizing agents. Carbon oxides. Nitrogen oxides (NOx). ne under normal processing. 11. TOXICOLOGICAL INFORMATION Acute toxicity Component Information Chemical Name LD50 Oral LD50 Dermal LC50 Inhalation Glycerol mg/kg Rat 4090 mg/kg Mouse 10 g/kg Rabbit 570 mg/m³ Rat 1 h Page 4 / 7

34 AP Storage Buffer Revision Date 23-Jul-2005 Chronic toxicity Carcinogenicity There are no known carcinogenic chemicals in this product. Legend: Irritation Corrosivity Sensitization Neurological effects Mutagenic effects Reproductive effects Developmental effects Target Organ effects May cause irritation of respiratory tract. May cause skin irritation in susceptible persons. Irritating to eyes. ne. ne. ne. ne. ne. ne. ne. 12. ECOLOGICAL INFORMATION Ecotoxicity Contains no substances known to be hazardous to the environment or not degradable in waste water treatment plants. Persistence/Degradability Bioaccumulation/ Accumulation Mobility in Environmental Media Inherently biodegradable. Does not bioaccumulate. Soluble. Glycerol log Pow = DISPOSAL CONSIDERATIONS Waste Disposal Method Contaminated packaging US EPA Waste Number Dispose of in accordance with National, State and Local regulations. Empty containers should be taken for local recycling, recovery or waste disposal. ne. 14. TRANSPORT INFORMATION DOT t regulated. Page 5 / 7

35 AP Storage Buffer Revision Date 23-Jul REGULATORY INFORMATION International Inventories Chemical Name TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS Glycerol X X - X - X X X X X TSCA DSL NDSL EINECS ELINCS ENCS CHINA KECL PICCS AICS USA Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product contains no chemical or chemicals which are subject to the reporting requirements of the Act and and Title 40n of the Code of Federal Regulations, Part 372. SARA 311/312 Hazardous Categorization Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard Yes Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61) This product contains the following HAPs: ne State Regulations California Proposition 65 This product contains the following Proposition 65 chemicals: ne State Right-to-Know Chemical Name Massachusetts New Jersey Pennsylvania Illinois Rhode Island Glycerol X X X Legend: X - Listed Page 6 / 7

36 AP Storage Buffer Revision Date 23-Jul-2005 Canada WHMIS hazard class n-controlled 16. OTHER INFORMATION Prepared By Revision Date Revision summary Product Stewardship 23 British American Blvd. Latham, NY Jul-2005 Disclaimer The information provided on this MSDS is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other material or in any process, unless specified in the text. End of MSDS Page 7 / 7