Plant Growth and Cultivation
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- Candace Lane
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1 Welcome to Issue 21 of the PSI NEWS This Issue is dedicated to Plant Growth and Cultivation PSI offers a suite of products for plant growth and cultivation all of which highly provide precise and stringently controlled growth conditions. LED Light Sources Research Greenhouses Growth Rooms Growth Chambers Incubated Shakers
2 LED light technology Control of light intensity and spectral quality is essential for optimal plant growth and biomass production. PSI uses mainly LED light technology to optimize light conditions. Key Features of LED Light Sources Precise light flux and waveband control Rapid activation enabling microsecond light pulses Precise ramping of light intensities Uniform homogeneity of irradiance Focused to maintain light intensity at different distances High incident irradiance up to 5000 μmol.m -2.s -1 Ability to lighting the side of the canopy Incremental control from 0 to 100% of maximum Minimal heat load on plants Durable, low power requirement Long life span of 6-7 years LEDs Provide Unique and Precise Light Quality Control Sun light simulation Cloudy sky imitation Dawn and Dusk imitation Specific pigment excitation Stimulation of individual phytochromes Simulation of Red and Far-red ratio as is in the canopy
3 Comparison of Arabidopsis Growth under Different Light Sources Experimental setup: 24 day old Arabidopsis 12/12h light regime 22/20 C, 55% humidity Light intensity 150 μmol.m -2.s -1 Mean and standard deviation presented (n=10) CW-FAR Cool white LED with Far-red WW-FAR Warm white LED with Far-red FT Fluorescent tube R-B-FAR Red and Blue LEDs BW-FAR Brightness cool white LED with Far-red Total chlorophyll content (µg/ml) NDVI values Rosette area (mm 2 ) 0,7 ChlA ChlB 0,6 0,5 0,4 0,3 0,2 0,1 0 0,87 0,86 0,86 0,85 0,85 0,84 0,84 0,83 0,83 0,82 0, White LEDs with Far-Red Brightness White LED with Far-Red Fluorescent Tubes Blue, Red, Far-Red LEDs
4 LED Light Sources LED LIGHT SOURCE SL 3500 SL 3500 self contained high-performance LED panels can operate in multiple regimes: flash, continuous, modulated light, or with user-defined modes of action. They can also complement other PSI instruments such as FluorCams, Photobioreactors, FytoScopes. Technical Specifications Light intensity up to 3000 μmol.m -2.s -1 Uniformly homogenous illumination Manual or software control Multicolor LED spectra from UVA to far-red Single, Bi- and Tri-Color versions available Precise control of light intensity and quality Microsecond pulses enabled Available Panels mm mm mm Applications Plant research and biomedicine Light signaling studies Plant handling under dim green light LED FYTO-PANELS LED Fyto-Panels were developed for illumination of large areas in growth chambers with a uniform light source. Panels are delivered with a control unit for regulation of timing, light intensity and spectral quality. Technical Specifications PAR μmol.m -2.s -1 (at 100 cm) Uniformly homogenous illumination Standard version: Cool white LEDs with far-red Optional Multicolor LED spectra available LED panels are cooled Precise control of light intensity and quality Independent control of each LED channel/color Light modes enable ramping/cloudy sky simulation etc. Available Sizes mm mm Combination of available sizes Applications Controlled uniform irradiance Plant research and biomedicine Agricultural and biotechnology research
5 LED Light Sources LED LIGHT BARS Individual LED light bars are designed for mounting in greenhouses and growth chambers for supplementary illumination to enhance plant growth. LED light bars may also be used as alternative light ing options to current sources. Technical Specifications PAR up to 500 μmol.m -2.s -1 (at 100 cm) White LEDs White LED enriched by far-red Available Sizes Maximal bar length 4 m Minimal bar length 20 cm Customized formats CULTIVATION SHELVES Cultivation Shelves provide modular cultivation spaces within growth rooms to maximise growth space usage. Each shelf is irradiated from by LED panels that are directly incorporated into the base of the shelf above. Each modular system is delivered with PSI s Light Controller for user-friendly programming of timing, light intensity and spectral quality. Technical Specifications PAR up to 250 μmol.m -2.s -1 (at 30 cm) Uniformly homogenous illumination White LED illumination with far-red LEDs Precise control of light intensity and quality Independent control of each LED channel Light modes enabling ramping/cloudy sky simulation etc. Shelves Maximum capacity of 8 shelves per unit Height adjustable up to 25 mm Easily removable Available Configurations* CS 250/300_5_3 CS 250/300_5_2.4 CS 250/200_7_4.2 CS 250/200_7_3.4 (*) PAR/at distance/number of shelves/cultivation area (m 2 ) Applications Maximizing space for cultivation in growth rooms Large scale experiments
6 Research Greenhouses Key Features Customized Designs Online control and monitoring of environment Regulation of: CO 2 Level Relative Humidity Temperature Lighting Central and remote control Individual greenhouse sectors may be controlled separately Automatic watering and light/dark control of tables Conventional and/or adiabatic cooling High-performance air-conditioning (air circulation, air exchange, humidity level control) Special regime for inner air recirculation (minimum fresh air required) Double or triple layer glass for maximum energy efficiency Software-regulated external shading system for heat/light control Smart Lighting Solutions Software control of light intensity and cycles Precise irradiance in gradual steps: LEDs controlled from 0 % to 100 % output Sodium/ Metal-Halide lamps controlled from 30 % to 100 % Homogeneous irradiance by high-intensity LEDs Height-adjustable LED light bars Cost-effective and energy efficient light sources
7 Growth Rooms PSI provides customized growth rooms designs. Key Features Relative Humidity between 40% to 90% Temperature range -5 C to +40 C Gas-tight rooms Gas mixing for CO 2 regualtion and exchange Online computer control Cultivation Systems Cultivation Banks (page8) Walk-In Fytoscope (page 9) Cultivation Shelves (page 5) Control & Setting User friendly GUI 10.5" LCD color touch screen Up to 100 user defined protocols Program changes in 1 min steps Parameters changes in gradual steps Real time data visualization Graphical/table data view Remote control Ramping modes cloudy sky simulation etc. Warning messages via SMS USB port Precise Control of Irradiance Red and Far-red enriched LED light system for optimal plant growth Uniform LED illumination Incrementall changes in irradiance from 0 to 100% Ramping modes, cloud simulation etc. Natural light simulation
8 Growth Rooms Cultivation Banks Sophisticated Air Ventilation System Extremely low air flow up to 0.25 m/s Minimal heat load in chamber Zero water condensation Light-independent regulation of temperature Uniformly homogenous environment Customizable Cultivation Banks CO 2 regulation Different light sources available (LED with different λ /UV/ fluorescent tubes) Light radiation monitoring Chlorophyll fluorescence module Variable numbers of Cultivation Banks More than 12 different temperatures and light settings in one cultivation chamber Each Cultivation Bank is independently controlled Suitable for multiple or parallel experiments Uniform homogeneity of set parameters Sophisticated air ventilation system LED light technology for optimal plant growth Easy programming & remote control Wide Range of Applications In vitro and soil cultivation of plants Suspension culture cultivation Multiple simultaneous physiological studies Phenotypical analysis (Arabidopsis) Vernalization (Brassica, Hordeum) Dusk and Dawn response simulation Key Features Individual control of multiple experiments Temperature range +2 C to +45 C PAR up to 1500µmol.m-2.s-1 at 30cm Relative Humidity 35-80% Sophisticated but user friendly software
9 Growth Rooms Walk-In Fytoscope Walk-In FytoScope Provides Unique system of temperature control Prevention of water condensation Uniform homogeneity of set parameters Specially assembled LED light technology Customizable to user-specifications Remote control Wide Range of Applications Large scale experiments In vitro / soil cultivation High humidity cultivation (Oryza) Vernalization (Brassica, Hordeum) Homogeneity of growth (phenotypical analysis) PSI Innovative Solution. Our temperature exchanger enables: Precise temperature regulation not affected by light cycle regulation Uniform homogeneity of temperature and humidity Prevention of water condensation Customize Your Fytoscope Chamber size according to your needs Multiple shelves to meet your application Shelving may be fixed or motorized Various light sources available (LEDs with different wavelengths, UV, fluorescent tubes) Innovative Technology Allows Temperature extremes of -5 C to +45 C during full light exposure! Light intensity up to 3,000 μmol.m -2.s -1 Humidity control from % (guaranteed from +5 C)
10 Growth Chambers Step-In FytoScope Step-In FytoScope Provides Multi-color LED light system suitable for light dependent experiments Innovative technology for stress and climate studies Dimensions customizable for tree experiments Precise heat and humidity regulation Prevention of water condensation Temperature not affected by light cycle regulation Easy programming and remote control Example Applications In vitro/soil cultivation Cultivating large plants and trees (0.9 m m ) Extreme humidity cultivation (Oryza) Vernalization (Brassica, Hordeum) Climate studies Stress responses Light dependent experiments (Study of light signaling pathways, shade avoidance responses etc.) Customize Your FytoScope Gas mixing system for CO 2 control Multiple adjustable shelves Chlorophyll fluorescence measurement module Online monitoring of photosynthetic performance Different light sources: LED with different wavelengths / UV / fluorescent tubes Innovative Technology Allows Temperature extremes -5 C / +45 C with ensured full light exposure! Light intensity up to 3,000 μmol.m -2.s -1 Dynamic multispectral irradiance Large plants and trees cultivation
11 Growth Chambers Small Footprint FytoScopes Growth chambers for cultivation of higher plants and suspension cultures in limited space. Technical Features Small footprint ideal for limited space Temperature control C Uniform LED lighting for optimal plant growth Two light color versions: (a) white + far-red (b) red + green + blue + far-red Control of light intensity and spectral quality Air exchange Applications Suitable for small scale experiments Plant growth in soil and in-vitro Cultivation of suspension cultures Space for dark adaptation and canopy simulation Customizable Features CO 2 regulation Temperature upgrade +7 to 55 C Light intensity upgrade up to 1500 μmol.m -2.s -1 RH control Integrated devices: Orbital shaker Chlorophyll Fluorescence module Precise PC software control of protocols, additional functions such as ramping modes, cloudy sky simulation etc. Control and Settings Digital display Sensor for temperature and optionally for RH Real time data collection and visualization Intuitive programming for: timing, temperature, light intensity and light quality Diurnal cycling Incremental ight intensity control % FYTOSCOPE CHAMBER FS 130 Space saving solution for the lab Easily adjustable shelf Inner space: cm (D W H) Internal volume: 124 Liters Light intensity: up to 1500 μmol.m -2.s -1 Space for light independent sample incubation Temperature Range: +7 C to +55 C (lights OFF) +10 C to +55 C (lights ON) FYTOSCOPE CHAMBER FS 360 Cultivation chamber accessible from the top One large or 2 smaller cultivation areas Internal volume: 290 Liters Light intensity up to 300 μmol.m -2.s -1 upgradable to 600 μmol.m -2.s -1 Temperature Range: +15 C to +40 C Temperature upgrade is not available
12 Incubated Shakers AlgaeTrons AlgaeTrons are floor standing incubators with small footprint size developed for optimizing cyanobacteria and algae cultivation. Technical Features Small size suitable for every lab space Temperature control C LED illumination (cool or warm white) Control of light intensity and spectral quality Air exchange Customizable Features Integrated orbital shaker CO 2 regulation Relative humidity control Temperature upgrade +7 C to +55 C Light intensity upgrade up to 1500 μmol.m -2.s -1 Multicolor LED combination (RED, GREEN, BLUE) Precise PC software control of protocols and functions such as ramping modes, cloudy sky simulation etc. Applications Precise Cyanobacterial and Algal cultivation Incubation of in-vitro cultures in flasks/dishes Suitable for small scale experiments Control and Settings Digital display of settings and parameters Real time data collection and visualization Intuitive programming for timing, temperature, light intensity and spectral quality Light intensity range % ALGAETRON AG 130-ECO Most economical space-friendly cultivation Easily adjustable shelf Inner space: cm (D W H) Internal volume: 124 Liters Space for light-independent sample incubation Light Intensity: up to 1000 μmol.m -2.s -1 (cool white) up to 500 μmol.m -2.s -1 (warm white) ALGAETRON AG 230 Three independently illuminated shelves Inner space: cm (D W H) Internal volume: 265 Liters Space for light-independent sample incubation 1 to 3 orbital shakers Light intensity: top shelf up to 500 μmol.m -2.s -1 lower shelves up to 100 μmol.m -2.s -1
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