Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK ccredited to Salford Royal Hospital Contact: Louise Bell Eccles New Road Tel: +44 (0) Salford louise.bell@srft.nhs.uk M6 8HD Website: United Kingdom below s covered by the organisation and their relevant activities Laboratory locations: details ctivity code Salford Royal Hospital Eccles New Road Salford M6 8HD United Kingdom Local contact Louise Bell Routine automated Chemistry General / Special Chemistry Toxicology Department of Biochemistry Essential Services Laboratory Wigan Royal lbert Infirmary Wigan Lane Wigan WN1 2NN United Kingdom Local contact Louise Bell Routine automated Chemistry B ssessment Manager: GB2 1 of 10

2 Schedule of ccreditation United Kingdom ccreditation Service ccredited to DETIL OF CCREDITTION HUMN BODY FLUIDS Direct Chemilluminesence using Siemens Centaur XP with specific reference to: FP SOP BIO-S--310 Beta-HCG SOP BIO-S--313 & B C199 SOP BIO-S--307 C125 SOP BIO-S--145 C153 SOP BIO-S--309 CE SOP BIO-S--311 Cortisol SOP BIO-S--314 & B Digoxin SOP BIO-S--315 & B Ferritin SOP BIO-S--316 & B Folate SOP BIO-S--317 & B Follicle Stimulating Hormone SOP BIO-S--318 Free T3 SOP BIO-S--283 Free T4 SOP BIO-S--281 & B Luteinising Hormone SOP BIO-S--319 Macroprolactin SOP BIO-S-E-18 Oestradiol SOP BIO-S-E-312 ssessment Manager: GB2 Page 2 of 10

3 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS Progesterone SOP BIO-S--321 Prolactin SOP BIO-S--320 PS SOP BIO-S--331 PTH SOP BIO-S--332 Thyroid Stimulating Hormone SOP BIO-S--282 & B Testosterone (Male) SOP BIO-S--335 Troponin I SOP BIO-S--330 & B Vitamin B12 SOP BIO-S--152 & B Vitamin D SOP BIO-S--336 Ion Specific Electrodes using Siemens dvia 2400 () or 1800 (B) with specific reference to: Urine also Chloride SOP BIO-S--254 & B Urine also Potassium SOP BIO-S--298 & B Urine also Sodium SOP BIO-S--299 & B Spectrophotometry using Siemens dvia 2400(Site ) or 1800(Site B) with specific reference to: Urine also lcohol SOP BIO-S--294 & B lpha-1-antitrypsin SOP BIO-S--247 lbumin SOP BIO-S--286 & B ssessment Manager: GB2 Page 3 of 10

4 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS lanine aminotransferase SOP BIO-S--244 & B spartate aminotransferas SOP BIO-S--250 lbumin SOP BIO-S--278 & B lkaline phosphatase SOP BIO-S--245 & B mmonia SOP BIO-S--248 & B Urine also mylase SOP BIO-S--249 & B nti-streptolysin antibody* SOP BIO-S--274 Benzodiazepine group SOP BIO-S--292 Beta-2-Microglobulin SOP BIO-S--251 Urine also Calcium SOP BIO-S--252 & B Cannabis screen SOP BIO-S--293 Carbamazepine SOP BIO-S--253 Ceruloplasmin SOP BIO-S--337 Cholesterol SOP BIO-S--255 & B Conjugated bilirubin SOP BIO-S--263 & B Creatine kinase SOP BIO-S--256 & B Urine also Creatinine SOP BIO-S--258 & B Urine also Enzymatic Creatinine SOP BIO-S--265 & B C-reactive protein SOP BIO-S--259 & B ssessment Manager: GB2 Page 4 of 10

5 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS Fructosamine SOP BIO-S--266 Gamma GT SOP BIO-S--267 & B Gentamicin SOP BIO-S--268 & B Glucose SOP BIO-S--269 & B CSF only Glucose SOP BIO-S--269 HDL-cholesterol SOP BIO-S--264 & B Iron SOP BIO-S--279 & B Immunoglobulin SOP BIO-S--275 Immunoglobulin G SOP BIO-S--276 Immunoglobulin M SOP BIO-S--277 Lactate SOP BIO-S--280 & B CSF only Lactate SOP BIO-S--280 Lactate dehydrogenase SOP BIO-S--300 & B Lithium SOP BIO-S--284 Urine also Magnesium SOP BIO-S--285 & B Paracetamol SOP BIO-S--243 & B Phenytoin SOP BIO-S--287 & B Urine also Phosphate SOP BIO-S--301 & B Salicylate SOP BIO-S--302 & B ssessment Manager: GB2 Page 5 of 10

6 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS Theophylline SOP BIO-S--291 & B Tobramycin SOP BIO-S--270 B Total bilirubin SOP BIO-S--303 & B Total CO2 SOP BIO-S--257 & B CSF only Total protein SOP BIO-S--334 Urine also Total protein SOP BIO-S--334 & B Transferrin SOP BIO-S--306 & B Triglycerides SOP BIO-S--304 & B Urine also Urate SOP BIO-S--297 & B Urine also Urea SOP BIO-S--296 & B Valproate SOP BIO-S--290 & B Vancomycin SOP BIO-S--289 B tomic bsorption Spectrometry using Varian Spectra with reference to: Copper SOP BIO-S-G-34 Selenium SOP BIO-S-G-27 Zinc SOP BIO-S-G-34 ssessment Manager: GB2 Page 6 of 10

7 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS Enzyme immunoassay with chemiluminescence Immulite 2000 with reference to: CTH SOP BIO-S-E-46 Nt-pro BNP SOP BIO-S-E-72 nti-tpo ntibodies BIO-S-E-68 DHE-sulphate SOP BIO-S-E-49 Growth hormone IGF1 SOP BIO-S-E-50 SOP BIO-S-E-58 Sex hormone binding globulin SOP BIO-S-E-54 Urine & Faeces only Porphyrins HPLC with fluorometry using Knauer K Pump HPLC with Shimadzu Fluorescence Detector SOP BIO-S-E-28 & 27 Plasma / Red Blood Cells only Porphyrins Perkin Elmer LS50B Fluorimeter SOP BIO-S-E-34 & 35 Urine also Osmolality Vitech Osmometer 3300 SOP BIO-S-S-09 Fluids only TU Protein Beckman Paragon electrophoresis system SOP BIO-S-G-37 & B Porphobilinogen (Qualitative) screen Manual Ion Exchange Chromatography SOP BIO-S-E-26 Porphobilinogen (Quantitative) + α-aminolaevulinic acid Elmer Lambda 25 UV spectrophometer BIO-S-E-32 ssessment Manager: GB2 Page 7 of 10

8 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS Waters Liquid Chromatography with Mass Spectrometry (premier Quattro) Blood only ndrostenedione SOP BIO-S-E-62 Blood only Ciclosporin SOP BIO-S-G-33 Blood only Sirolimus SOP BIO-S-G-33 Blood only Tacrolimus SOP BIO-S-G-33 Testosterone (Female) SOP BIO-S-E-62 Urine 5HI SOP BIO-S-E-67 Cortisol SOP BIO-S-E-67 Total Metadrenalines SOP BIO-S-E-36 Waters Liquid Chromatography with Mass Spectrometry (TQD) Specific mphetamine Class Screen SOP BIO-S-D-36 Specific Benzodiazepine Screen SOP BIO-S-D39, 35 Standard Drugs of buse: Opioids, Opiates, Cotinine, mphetamine, Cocaine metabolite (BEC), Buprenorphine, Nor Buprenorphine BIO-S-D-32, 35 &37 ssessment Manager: GB2 Page 8 of 10

9 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS Extended toxicology screen Stimulant/Miscellaneous drugs screen: Benzylpiperizine (BZP), Benzoylecognine (BEC), Ketamine, Tramadol, LSD metabolite and Phencyclidine (PCP) Vacumaster solid phase extraction tank Waters GCMS and LC/MS/MS with systematic toxicological analysis software SOP BIO-S-D-4, BIO-S-D-44, BIO- S-D-17 GHB Screen SOP BIO-S-D-45 Vitamin and E * SOP-BIO-S-G-43 Cannabis confirmation 11-nor-delta9-THC-9- Carboxylic acid Capillary Gas Chromatography & Mass Spectroscopy (Perkin Elmer) SOP BIO-S-D-26,SOP BIO-S-D-29 ph Jenway 3505 meter SOP BIO-S-E-40 Radiometer BL 820 () or Roche b221 (B) with reference to: Whole Blood only Blood gases / acid base SOP BIO-S-S-01 (potentiometry & amperometry) Whole Blood only Blood gases / acid base SOP BIO-S-S-16 (potentiometry & amperometry) B Whole Blood only Co-oximetry Carboxy-Hb, Met- Hb, Oxy-Hb SOP BIO-S-S-16 (spectrophotometry) Whole Blood only Co-oximetry Carboxy-Hb, Met- Hb, Oxy-Hb SOP BIO-S-S-17 (spectrophotometry) B CSF Xanthochromia Elmer Lambda 25 UV spectrophometer SOP BIO-S-G-25 ssessment Manager: GB2 Page 9 of 10

10 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN BODY FLUIDS Plasma Metadrenaline Normetadrenaline Manual ELIS using documented in-house procedure SOP BIO-S-E-54 Immunodiagnostik ELIS using the DS2 Faeces only Calprotectin SOP-BIO-S-G-42 Serum Free Light chains Turbidimetry using the Binding site Spa Plus SOP IMM-S-I-29 Capillary Electrophoresis using the Helena V8 and SS3 and SS4* system Urine also Serum protein and Urine Bence Jones protein (immunoglobulin light chains) SOP IMM-S-I-05 / SOP IMM-S-I-06 Serum and CSF Blood Brain Barrier Studies- lbumin and IgG Binding site Spa Plus SOP BIO-S-I-05 Serum and CSF Oligoclonal Bands -IgG Pharmatcia Biotech Isoelectric focussing SOP IMM-S-I-04 END ssessment Manager: GB2 Page 10 of 10

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