De Bortoli Wines Yarra Valley Vineyards. Using Biological farming practices to grow the best grapes efficiently, safely and sustainably

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De Bortoli Wines Yarra Valley Vineyards Using Biological farming practices to grow the best grapes efficiently, safely and sustainably

Soil Management using Biological Farming Techniques What is Biological farming? Why we implement it on our farms How we utilise and encourage organisms to work for us to improve soil health and vine health Outcomes since implementation in spring 2008

What is Biological Farming? Biological Farming is seeking a biological remedy before we consider a physical or chemical approach. We amend our systems to enable living organisms to alter a situation to our desired state. We monitor as best we can the impact of Biological farming.

Soil Biology Microbiology Macrobiology Bacteria Fungi Protozoa Nematodes Worms Ants Grubs Plants

Why do we use biology to manage soil characteristics? We considered the ecosystems where wild vines grow naturally What was our farms natural system, and what state is it in now? How can we best reconcile the differences between the natural system and our agriculture system to allow each vine to best express its wine characteristics

What dictates the default state of an ecosystem? Geology Soil Type Temperature range Rainfall Seasonality Topography Aspect Biology types and forms are selected based on system parameters

How we encourage soil biology to grow better grapes We create the best physical environment possible for desired biology growth We start at the bottom of the food chain We push the system via inoculum and food sources We create symbiotic relationships wherever possible We try to minimise acts that destroy the desired biology

Soil Physical State Structure Reduce compaction Porosity Permanent vegetation coverage Water movement and retention Minimal cultivation

Compaction minimisation Controlled traffic rows Doing more than one operation Double row equipment Over row equipment Soil Puffer

Inoculum and Food sources Compost Compost Tea Bacterial Foods Fungal Foods Protozoa Nematodes Earthworms

Compost We try to make a fungal compost We primarily use plant materials Recipe is 25% Grape marc (high N), 15% Hay (med C:N ratio) and 60% Wood chips (High C) We let the temp get to 65 degrees and then turn it so it has enough O2 We turn until the temp stops climbing to 65 degrees this usually takes 10-16 weeks

Cost of Making Compost Grape Marc $0 25% $0 Hay $0 to $10 15% $1.50 Wood chips $8 to $14 60% $8.50 Making Piles $2.50 Turning Piles $0.50 Total for 1 M cubed of Compost = $13.00

Compost Tea Uses biology in Compost and encourages that biology to multiply in a liquid phase Must have adequate oxygen levels to allow anerobic organisms to dominate Must add foods for organisms Can brew at ambient or elevated temps up to about 32 degrees

Compost Tea Recipe & Cost 20L of compost For 1000L $0 4 handfuls of forest fungi $0 3L of Kelp food $36 3L of Fish food $30 400ml of Aloe Vera juice $4 Power 24 to 48 hours $10 Total is $80 for 1000L (8 cents a L)

Compost tea application 40L Tea/Ha $3.20 5L Kelp $20 5L Fish $9 Total Cost $/Ha $32.20

The Theory Bacteria have a C:N ratio of 5C molecules to 1N molecule. Protozoa Flagelates consume bacteria, but their natural state is 30C:1N molecule so to get 30C they consume 6 bacteria but have excess of 5N. The extra N is expelled as NH4. For other nutrients such as Ca, P, K they are expelled in chelated form, plant available but not leachable. Protozoa consume 10000 bacteria a day in good conditions releasing 8000N molecules. This equates to 7ng per cm cubed of root soil per day, plants only require 0.2ng of N per day. Excess N is used by bacteria in healthy soils for growth, so the N in healthy soils stays in living organisms and no N is lost from the system.

Tracking changes & outcomes Soil physical, biological and chemical properties Vine health and petiole nutrients Try to identify trends that have occurred Same blocks, same time of year, same sampling method, same lab

Compaction 0 Drip Line Wheel Track Mid row Wheel Track Drip Line 0 Drip Line Wheel Track Mid row Wheel Track Drip Line 500 500 1000 1000 1500 2000 70-80 cm 25-35 cm 10-15 cm 1500 2000 70-80 cm 25-35 cm 10-15 cm 2500 2500 3000 Non Travel Rows 3000 Travel Rows 3500 3500

Organic matter 6 5.75 5.5 5.25 5 4.75 4.5 4.25 4 3.75 3.5 3.25 3 2.75 2.5 2.25 2 1.75 1.5 1.25 1 0.75 0.5 0.25 0 Organic Matter % 2010 2009 2010 2011 2012 2013

ph 7.25 7 6.75 6.5 6.25 6 5.75 5.5 5.25 5 4.75 4.5 4.25 4 3.75 3.5 3.25 3 2.75 2.5 2.25 2 1.75 1.5 1.25 1 0.75 0.5 0.25 0 Soil ph 2008 2009 2010 2011 2012 2013

Soil Fungi Biomass 450 Average total Soil Fungi Biomass ug/g 400 350 300 250 200 150 100 50 0 Autumn 2008 Spring 2008 Autumn 2010 Spring 2010 Autumn 2011 Spring 2011 Autumn 2012 Spring 2012 Autumn 2013 Spring 2013 Autumn 2014

Soil fungi biomass to Bacteria biomass ratio 9 Soil Fungi Biomass to Bacteria Biomass Ratio 8 7 6 5 4 3 2 1 0 Autumn 2008 Spring 2008 Autumn 2010 Spring 2010 Autumn 2011 Spring 2011 Autumn 2012 Spring 2012 Autumn 2013 Spring 2013 Autumn 2014

Total Soil Ca 4500 Average Soil Calcium for All Blocks 4000 3500 3000 2500 2000 1500 1000 500 0 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Soil Available Ca 1800 Average Soil Available Calcium For All Blocks 1600 1400 1200 1000 800 600 400 200 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Petiole Ca 1.8 Average Petiole Calcium For All Blocks 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Spring 2009 Available soil Ca 27% Non Available soil Ca 73% Spring 2013 Available soil Ca 41% Non Available soil Ca 59%

Total Soil P 600 Average Soil Phosphorus for All Blocks 500 400 300 200 100 0 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Soil available P 14 Average Soil Available Phosphorous For All Blocks 12 10 8 6 4 2 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Petiole P 0.6 Average Petiole Phosphorus For All Blocks 0.5 0.4 0.3 0.2 0.1 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Available soil P 1% Spring 2009 Non Available soil P 99% Available soil P 2% Spring 2013 Non Available soil P 98%

Total Soil K 1200 Average Soil Potassium For All Blocks 1000 800 600 400 200 0 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Soil Available K 200 Average Soil Available Potassium For All Blocks 180 160 140 120 100 80 60 40 20 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Petiole K 5 Average Petiole Potassium For All Blocks 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Available soil K 12% Spring 2009 Non Available soil K 88% Available soil K 13% Spring 2013 Non Available soil K 87%

Soil Available Nitrate 30 Average Soil Available Nitrate For All Blocks 25 20 15 10 5 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Soil Available Ammonium 40 Average Soil Available Ammonium For All Blocks 35 30 25 20 15 10 5 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Petiole N 2.5 Average Petiole Nitrogen For All Blocks 2 1.5 1 0.5 0 Spring 2008 Spring 2009 Spring 2010 Spring 2011 Spring 2012 Spring 2013

Soil Available Ammonium 12% Soil Available N Spring 2008 Soil Available Nitrate 88% Soil Available N Spring 2013 Soil Available Ammonium 78% Soil Available Nitrate 22%

Earthworms Mil/Ha 14 Earthworms (millions) per hectare 12 10 8 6 4 2 0

Vine Balance We monitor internode length We monitor cane length for mm per infloresence We monitor cane length and mass in mm per gram

D3 Sauv Blanc Replanted after 25% was burnt in 2009 Flat Alluvial soil Duplex soil with heavy clay and sodic sub soil Water logs very easily Compaction layer at 40 to 50cm We have used soil aerator and banded compost under vine at 1m3/20m of row

D3 Sauv blanc 2009 2013/14 Organic Matter 4.9 6.8 Soil ph 5.84 6.23 Earthworms 0 65 Soil Fungi Biomass 216 236 Fungi:Bacteria 0.16 2.31 Total Na 182 113 Soil Avail Na 153 63 Petiole Na 0.04 0.03 Total Ca 2215 2929 Soil Avail Ca 874 1002 Petiole Ca 0.81 0.84 Total P 250 442 Soil Avail P 0.5 8.7 Petiole P 0.16 0.31 Total K 975 1560 Soil Avail K 98 324 Petiole K 5.54 4.26 Soil Avail NO3 29.7 6.7 Soil Avail NH4 20.4 23 Petiole N 1.33 2.62

Things we are still working on: Using biology on foliage to protect against downy and powdery mildews Establishing permanent undervine plants Increasing the length and activity of soil biology in the spring and autumn Improving nematode diversity and numbers Improving mycorrhiza establishment