Sample Prep - Solid Phase Extraction

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Atoll TM - Polymer based Atoll TM is a comprehensive product range based upon porous polymers consisting of ultra pure spherical particles with strictly controlled particle & pore size distribution. Atoll TM is the latest generation of polymer that does not shrink or swell with a range of standard solvents. Typical Atoll procedure Atoll TM can be used through a wide range of ph (1-14) and is compatible with all solvents and existing samples. It has a very high surface area, Atoll TM XC exhibiting the highest surface area currently available on market (1500 sq M /g) approximately three times greater loading capacity than traditional silica sorbents. The higher capacity of Atoll TM allows for smaller bed masses. Interchim bonding technology ensures greater batch to batch reproducibility for the Atoll TM family of products, therefore there is no longer a need for batch reservations. Our products subsequently achieve superior recovery rates relative to traditional media, exhibiting excellent reproducibility & consistency. The Atoll TM family of products : Atoll Xtrem TM Capacity (XC) - Atoll TM Xtrem Capacity Wide Pore (XWP) - Atoll TM Hydrophilic (ATH) - Atoll TM Environment (AEV) - Atoll TM Lipophilic (ATL) The Atoll TM range achieves accuracy of +/- 1% in mass per column thanks to a strictly controlled packing process. Atoll TM products are thoroughly quality control tested in-house to guarantee tracability. Products are supplied with an individual certificate detailing the specific production number and sorbent batch. Atoll TM columns are packed in a PEHD/Al bag for long term integral storage. This prevents potential damage from UV and moisture. Type Product code Sorbent Particle size Porosity Surface area Atoll Xtrem Capacity 30XC PSDVB 30 µm n.a. 1500 m 2 /g XC PSDVB 70 µm n.a. 1500 m 2 /g Atoll Xtrem Capacity Wide Pore XWP PSDVB 90 µm n.a. 1200 m 2 /g Atoll Hydrophile 30ATH n.a. 30 µm 70 Å 800 m 2 /g ATH n.a. 75 µm 70 Å 800 m 2 /g Atoll Environment AEV PSHEMA 75 µm 70 Å 800 m 2 /g Atoll Lipophile ATL PSDVB 100 µm 70 Å 800 m 2 /g.com - Please contact your local distributor A.43

Atoll TM XC columns - Polymer based Atoll TM Xtrem Capacity (XC) Atoll TM XC exhibits the highest surface area currently available on the market (1500 sq M /g), approximately three times greater loading capacity than traditional silica sorbents. Atoll XC improves, 2 to 3 fold, the retention of a compound of interest, compared to traditional silica. Atoll XC polymer is a versatile sorbent for the extraction and pre-concentration of non-polar, polar, acidic, basic or neutral compounds. º Std. 70 µm and 30 µm º ph stability : 1 to 14 º Pharmaceutical applications : drugs & metabolites in biological fluids º Environmental applications : PAH, PCB, carbamates, phenyl-ureas, acrylamide, glyphosate Weight Volume Qty Atoll XC Atoll 30 µm XC / PE frits 30 mg 1 ml 50 u XC-30/1 30XC-30/1 50 mg 1 ml 50 u XC-50/1 30XC-50/1 60 mg 1 ml 50 u XC-60/1 30XC-60/1 75 mg 1 ml 50 u XC-75/1 30XC-75/1 100 mg 1 ml 50 u XC-100/1 30XC-100/1 100 mg 3 ml 50 u XC-100/3 On request 150 mg 3 ml 50 u XC-150/3 On request 200 mg 3 ml 50 u XC-200/3 On request 250 mg 3 ml 50 u XC-250/3 On request 500 mg 6 ml 30 u XC-500/6 On request 50 mg LRC 15 50 u XC-50LRC 30XC-50LRC 75 mg LRC 15 50 u XC-75LRC 30XC-75LRC 100 mg LRC 15 50 u XC-100LRC 30XC-100LRC 200 mg LRC 15 50 u XC-200LRC On request / PTFE frits 30 mg 1 ml 50 u XC-30/1T 30XC-30/1T 50 mg 1 ml 50 u XC-50/1T 30XC-50/1T 60 mg 1 ml 50 u XC-60/1T 30XC-60/1T 75 mg 1 ml 50 u XC-75/1T 30XC-75/1T 100 mg 1 ml 50 u XC-100/1T 30XC-100/1T 100 mg 3 ml 50 u XC-100/3T On request 150 mg 3 ml 50 u XC-150/3T On request 200 mg 3 ml 50 u XC-200/3T On request 250 mg 3 ml 50 u XC-250/3T On request 200 mg 6 ml 30 u XC-200/6G On request 500 mg 6 ml 30 u XC-500/6G On request PP straight tube + 20µm PE frits LRC columns PP tube + 20µm PE frits PP straight tube + 20µm PTFE frits Glass columns Glass tube + 20µm PTFE frits A.44

Atoll TM ATH columns - Polymer based Atoll TM Hydrophilic (ATH) Atoll TM Hydrophilic contains proprietary chemical modifications that achieve unsurpassed compound extraction within 100% aqueous matrices. This technology facilitates mixed hydrophilic /hydrophobic interactions. º Std. 70 µm º ph stability : 2 to 12 º Pharmaceutical applications : drugs & metabolites in biological fluids º Environmental applications : PAH, PCB, carbamates, phenyl-ureas, acrylamide, glyphosate Weight Volume Qty Atoll ATH Atoll 30 µm ATH / PE frits 30 mg 1 ml 50 u ATH-30/1 30ATH-30/1 50 mg 1 ml 50 u ATH-50/1 30ATH-50/1 60 mg 1 ml 50 u ATH-60/1 30ATH-60/1 75 mg 1 ml 50 u ATH-75/1 30ATH-75/1 100 mg 1 ml 50 u ATH-100/1 30ATH-100/1 100 mg 3 ml 50 u ATH-100/3 On request 150 mg 3 ml 50 u ATH-150/3 On request 200 mg 3 ml 50 u ATH-200/3 On request 250 mg 3 ml 50 u ATH-250/3 On request 500 mg 6 ml 30 u ATH-500/6 On request 1000 mg 6 ml 30 u ATH-1G/6 On request 50 mg LRC 15 50 u ATH-50LRC 30ATH-50LRC 75 mg LRC 15 50 u ATH-75LRC 30ATH-75LRC 100 mg LRC 15 50 u ATH-100LRC 30ATH-100LRC 200 mg LRC 15 50 u ATH-200LRC On request / PTFE frits 30 mg 1 ml 50 u ATH-30/1T 30ATH-30/1T 50 mg 1 ml 50 u ATH-50/1T 30ATH-50/1T 60 mg 1 ml 50 u ATH-60/1T 30ATH-60/1T 75 mg 1 ml 50 u ATH-75/1T 30ATH-75/1T 100 mg 1 ml 50 u ATH-100/1T 30ATH-100/1T 100 mg 3 ml 50 u ATH-100/3T On request 150 mg 3 ml 50 u ATH-150/3T On request 200 mg 3 ml 50 u ATH-200/3T On request 250 mg 3 ml 50 u ATH-250/3T On request 500 mg 6 ml 30 u ATH-500/6T On request 1000 mg 6 ml 30 u ATH-1G/6T On request 200 mg 6 ml 30 u ATH-200/6G On request 500 mg 6 ml 30 u ATH-500/6G On request.com - Please contact your local distributor A.45

Atoll TM AEV columns - Polymer based Atoll TM Environment (AEV) Atoll TM AEV is a hydroxyethlmethacrylate - polystyrene polymer that supports the extraction of compounds weakly retained on C18. Atoll TM AEV exhibits high extraction flow of non-polar and mid-polar compounds that allows to clean and collect multi-residue samples in a single fraction prior to analysis. These characteristics identify this support as suitable to perform environmental extractions. º 75 µm º ph stability : 1 to 12 º Environmental applications : PAH, PCB, carbamates, phenyl-ureas Weight Volume Qty AEV / PE frits 30 mg 1 ml 50 u AEV-30/1 50 mg 1 ml 50 u AEV-50/1 60 mg 1 ml 50 u AEV-60/1 75 mg 1 ml 50 u AEV-75/1 100 mg 1 ml 50 u AEV-100/1 100 mg 3 ml 50 u AEV-100/3 150 mg 3 ml 50 u AEV-150/3 200 mg 3 ml 50 u AEV-200/3 250 mg 3 ml 50 u AEV-250/3 500 mg 6 ml 30 u AEV-500/6 1000 mg 6 ml 30 u AEV-1G/6 50 mg LRC 15 50 u AEV-50LRC 75 mg LRC 15 50 u AEV-75LRC 100 mg LRC 15 50 u AEV-100LRC 200 mg LRC 15 50 u AEV-200LRC / PTFE frits 30 mg 1 ml 50 u AEV-30/1T 50 mg 1 ml 50 u AEV-50/1T 60 mg 1 ml 50 u AEV-60/1T 75 mg 1 ml 50 u AEV-75/1T 100 mg 1 ml 50 u AEV-100/1T 100 mg 3 ml 50 u AEV-100/3T 150 mg 3 ml 50 u AEV-150/3T 200 mg 3 ml 50 u AEV-200/3T 250 mg 3 ml 50 u AEV-250/3T 500 mg 6 ml 30 u AEV-500/6T 1000 mg 6 ml 30 u AEV-1G/6T 200 mg 6 ml 30 u AEV-200/6G 500 mg 6 ml 30 u AEV-500/6G PP straight tube + 20µm PE frits LRC columns PP tube + 20µm PE frits PP straight tube + 20µm PTFE frits Glass columns Glass tube + 20µm PTFE frits A.46

Atoll TM ATL columns - Polymer based Atoll TM Lipophilic (ATL) Atoll TM ATL is a 100 µm PSDVB optimized for the extraction of hydrophobic compounds in a large variety of matrices and is an excellent alternative to high flow silica for crude samples. º ph stability : 1 to 14 º Applications : oils, muds, reactional environment after synthesis, Weight Volume Qty Atoll ATL / PE frits 30 mg 1 ml 50 u ATL-30/1 50 mg 1 ml 50 u ATL-50/1 60 mg 1 ml 50 u ATL-60/1 75 mg 1 ml 50 u ATL-75/1 100 mg 1 ml 50 u ATL-100/1 100 mg 3 ml 50 u ATL-100/3 150 mg 3 ml 50 u ATL-150/3 200 mg 3 ml 50 u ATL-200/3 250 mg 3 ml 50 u ATL-250/3 500 mg 6 ml 30 u ATL-500/6 1000 mg 6 ml 30 u ATL-1G/6 50 mg LRC 15 50 u ATL-50LRC 75 mg LRC 15 50 u ATL-75LRC 100 mg LRC 15 50 u ATL-100LRC 200 mg LRC 15 50 u ATL-200LRC / PTFE frits 30 mg 1 ml 50 u ATL-30/1T 50 mg 1 ml 50 u ATL-50/1T 60 mg 1 ml 50 u ATL-60/1T 75 mg 1 ml 50 u ATL-75/1T 100 mg 1 ml 50 u ATL-100/1T 100 mg 3 ml 50 u ATL-100/3T 150 mg 3 ml 50 u ATL-150/3T 200 mg 3 ml 50 u ATL-200/3T 250 mg 3 ml 50 u ATL-250/3T 500 mg 6 ml 30 u ATL-500/6T 1000 mg 6 ml 30 u ATL-1G/6T 200 mg 6 ml 30 u ATL-200/6G 500 mg 6 ml 30 u ATL-500/6G.com - Please contact your local distributor A.47

Atoll TM XWP columns - Polymer based Atoll TM Xtrem Capacity Wide Pore (XWP) Atoll TM XWP is a highly cross linked PSDVB exhibiting a very high surface area (1200 m 2 /g) and a 90 µm particle size. The apparent porosity of Atoll TM XWP makes it particularly suitable for protein & peptide cleaning from biologic fluids within a cut-off limit of ~ 500 KD. Its characteristics guarantee an excellent throughput and achieve efficient extractions without clogging. º ph stability : 1 to 14 º Applications : proteins and peptides in polar matrices, molecules with high molecular weight in all solvent types. Weight Volume Qty Atoll XWP / PE frits 30 mg 1 ml 50 u XWP-30/1 50 mg 1 ml 50 u XWP-50/1 60 mg 1 ml 50 u XWP-60/1 75 mg 1 ml 50 u XWP-75/1 100 mg 1 ml 50 u XWP-100/1 100 mg 3 ml 50 u XWP-100/3 150 mg 3 ml 50 u XWP-150/3 200 mg 3 ml 50 u XWP-200/3 250 mg 3 ml 50 u XWP-250/3 500 mg 6 ml 30 u XWP-500/6 50 mg LRC 15 50 u XWP-50LRC 75 mg LRC 15 50 u XWP-75LRC 100 mg LRC 15 50 u XWP-100LRC 200 mg LRC 15 50 u XWP-200LRC / PTFE frits 30 mg 1 ml 50 u XWP-30/1T 50 mg 1 ml 50 u XWP-50/1T 60 mg 1 ml 50 u XWP-60/1T 75 mg 1 ml 50 u XWP-75/1T 100 mg 1 ml 50 u XWP-100/1T 100 mg 3 ml 50 u XWP-100/3T 150 mg 3 ml 50 u XWP-150/3T 200 mg 3 ml 50 u XWP-200/3T 250 mg 3 ml 50 u XWP-250/3T 200 mg 6 ml 30 u XWP-200/6G 500 mg 6 ml 30 u XWP-500/6G PP straight tube + 20µm PE frits LRC columns PP tube + 20µm PE frits PP straight tube + 20µm PTFE frits Glass columns Glass tube + 20µm PTFE frits A.48

Atoll TM Bio-Ion Exchange columns - Polymer based Atoll TM Bio-Ion Exchange Atoll TM Bio-Ion Exchange bonded chemistry exhibits Ion exchange interaction and offers distinct selectivity to support the extraction and purification of proteins in aqueous matrices, such as biological fluids. Adsorbent vol. Volume Qty SPCE WPCE WPAE SPAE 0,5 ml 3 ml 50 u SPCE-0.5/3 WPCE-0.5/3 WPAE-0.5/3 SPAE-0.5/3 1 ml 3 ml 50 u SPCE-1X/3 WPCE-1X/3 WPAE-1X/3 SPAE-1X/3 2 ml 6 ml 50 u SPCE-2X/6 WPCE-2X/6 WPAE-2X/6 SPAE-2X/6.com - Please contact your local distributor A.49

Atoll TM 96 well plates - Polymer based Atoll TM is a comprehensive product range based upon porous polymers consisting of ultra pure spherical particles with strictly controlled particle & pore size distribution. Atoll TM is the latest generation of polymer that does not shrink or swell within a range of standard solvents. The Atoll TM family of products : Atoll Xtrem TM Capacity (XC) - Atoll TM Xtrem Capacity Wide Pore (XWP) - Atoll TM Hydrophilic (ATH) - Atoll TM Environment (AEV) - Atoll TM Lipophilic (ATL) See page A.43 to A.49 for relevant information about Atoll TM characteristics. Automated cleanup procedures are now an integral part of the modern laboratory. Atoll TM is available in a 96 well format with a 2 ml volume per well. This high quality unit provides rapid throughput of sample, within an automated process, whilst establishing excellent well-to-well consistency. Atoll TM 96 well plates utilise Interchim s state of the art automated weight machine. This packing technology guarantees unprecedented accuracy (+/- 1% mass per column) compared to the commonly utilised competitor volumetric systems. Atoll TM therefore raises the standards & client expectations for sample recovery /reproducibility. Atoll TM 96 well plates are QC tested in-house to guarantee tracibility. Products are supplied with an individual certificate detailing Mfg number, Sorbent batch number and specifications. An additional certificate is supplied that states real sorbent mass in each individual well. Atoll TM 96 well plates are packed in a PEHD/Al bag for long term integral storage. This prevents potential damage from UV and moisture. Atoll TM 96 well plates are manufactured from polypropylene, conform to a standard footprint (127,76 mm x 85,47 mm x 19,74 mm) and are compatible with existing systems on the market. Weight Qty Atoll XC Atoll 30XC Atoll ATH Atoll 30ATH 30 mg 1 u XC-30/WP20 30XC-30/WP20 ATH-30/WP20 30ATH-30/WP20 50 mg 1 u XC-50/WP20 30XC-50/WP20 ATH-50/WP20 30ATH-50/WP20 60 mg 1 u XC-60/WP20 30XC-60/WP20 ATH-60/WP20 30ATH-60/WP20 75 mg 1 u XC-75/WP20 30XC-75/WP20 ATH-75/WP20 30ATH-75/WP20 100 mg 1 u XC-100/WP20 30XC-100/WP20 ATH-100/WP20 30ATH-100/WP20 150 mg 1 u XC-150/WP20 n.a. n.a. n.a. 200 mg 1 u XC-200/WP20 n.a. n.a. n.a. Weight Qty Atoll AEV Atoll ATL Atoll XWP 30 mg 1 u AEV-30/WP20 ATL-30/WP20 XWP-30/WP20 50 mg 1 u AEV-50/WP20 ATL-50/WP20 XWP-50/WP20 60 mg 1 u AEV-60/WP20 ATL-60/WP20 XWP-60/WP20 75 mg 1 u AEV-75/WP20 ATL-75/WP20 XWP-75/WP20 100 mg 1 u AEV-100/WP20 ATL-100/WP20 XWP-100/WP20 A.50