efluor Organic Dyes
efluor Organic Dyes Catalog No. Description Clone Application Anti-Mouse efluor 450 Products 48-0042 Anti-mouse CD4 RM4-5 Flow Cytometry 48-0081 Anti-mouse CD8 53-6.7 Flow Cytometry 48-0441 Anti-mouse/human CD44 IM7 Flow Cytometry 48-0453 Anti-mouse CD45.1 A20 Flow Cytometry 48-0731 Anti-rat/mouse CD73 TY/11.8 Flow Cytometry 48-5321 Anti-mouse MHC class II M5/114.15.2 Flow Cytometry 48-5993 Anti-mouse IgD 11-26c (11-26) Flow Cytometry 48-0900 Anti-mouse CD90.1 HIS51 Flow Cytometry 48-0902 Anti-mouse CD90.2 53-2.1 Flow Cytometry 48-0032 Anti-mouse CD3 17A2 Flow Cytometry Anti-Human efluor 450 Products 48-0049 Anti-human CD4 RPA-T4 Flow Cytometry 48-0088 Anti-human CD8 RPA-T8 Flow Cytometry 48-0441 Anti-mouse/human CD44 IM7 Flow Cytometry 48-0149 Anti-human CD14 61D3 Flow Cytometry 48-0458 Anti-human CD45RA HI100 Flow Cytometry 48-0168 Anti-human CD16 CB16 Flow Cytometry 48-0037 Anti-human CD3 OKT3 Flow Cytometry 48-0199 Anti-human CD19 HIB19 Flow Cytometry efluor 450 Isotype Controls and Support Products 48-4724 Mouse IgG2a, K Isotype Control - Flow Cytometry 48-4732 Mouse IgG2b Isotype Control - Flow Cytometry 48-4301 Rat IgG1 Isotype Control - Flow Cytometry 48-4317 Streptavidin - Flow Cytometry 48-4321 Rat IgG2a - Flow Cytometry 48-4031 Rat IgG2b - Flow Cytometry 48-4714 Mouse IgG1 - Flow Cytometry 48-4888 Armenian hamster Ig isotype - Flow Cytometry Fluorescence Intensity 450/50 BP 600 500 400 300 200 100 0 400 420 440 460 480 Wavelength (nm) 500 520 540
Catalog No. Description Clone Application Anti-Mouse APC-eFluor 780 Products 47-0032 Anti-mouse CD3 (CD3e) 17A2 Flow Cytometry 47-0042 Anti-mouse CD4 RM4-5 Flow Cytometry 47-0081 Anti-mouse CD8 53-6.7 Flow Cytometry 47-0441 Anti-mouse/human CD44 IM7 Flow Cytometry 47-0454 Anti-mouse CD45.2 104 Flow Cytometry 47-0900 Anti-rat/mouse CD90.1 HIS51 Flow Cytometry 47-0902 Anti-mouse CD90.2 53-2.1 Flow Cytometry 47-5961 Anti-mouse TCR beta H57-597 Flow Cytometry 47-1171 Anti-mouse CD117 2B8 Flow Cytometry 47-0251 Anti-mouse CD25 PC61.5 Flow Cytometry Anti-Human APC-eFluor 780 Products 47-0038 Anti-human CD3 UCHT1 Flow Cytometry 47-0049 Anti-human CD4 RPA-T4 Flow Cytometry 47-0088 Anti-human CD8 RPA-T8 Flow Cytometry 47-0209 Anti-human CD20 2H7 Flow Cytometry 47-0279 Anti-human CD27 O323 Flow Cytometry APC-eFluor 780 Isotype Controls and Support Products 47-4888 Armenian Hamster IgG Isotype Control - Flow Cytometry 47-4714 Mouse IgG1, K Isotype Control - Flow Cytometry 47-4724 Mouse IgG2a, K Isotype Control - Flow Cytometry 47-4732 Mouse IgG2b Isotype Control - Flow Cytometry 47-4301 Rat IgG1 Isotype Control - Flow Cytometry 47-4321 Rat IgG2a Isotype Control - Flow Cytometry 47-4031 Rat IgG2b Isotype Control - Flow Cytometry 47-4317 Streptavidin - Flow Cytometry Fluorescence Intensity 780/60 BP 700 600 500 400 300 200 100 0 650 700 750 800 850 900 Wavelength (nm)
efluor Nanocrystals efluor Nanocrystals Introducing a NEW range of fluorochromes excited by the violet and UV laser for Flow Cytometry efluor 605 NC efluor 625 NC Narrow emission spectra requires minimal compensation More direct conjugates equals more choice and flexibility Fully compatible with standard organic fluorochromes High signal to noise ratios enable high quality analysis efluor Nanocrystals ( NC ) are high quantum yield nanocrystals that possess a core particle surrounded by a patented surface coating to reduce background staining and artifacts. efluor Nanocrystals are easy to use. Their unique physical and spectral properties make them excellent products for researchers performing multicolor applications. ebioscience offers a rapidly expanding portfolio of ready-to-use mouse and human reactive, and control antibody conjugates for flow cytometry. efluor nanocrystal conjugates are named for their emission wavelength. Our current offerings of efluor 605 NC and efluor 625 NC direct conjugates can be excited by either the violet or UV lasers; opening up a new range of versatile reagents for use with these lasers. for flow cytometry ebioscience New Releases October 2008 featuring CD3 (h) CD4 (h, m) CD8 (h, m) CD11b (h, m) CD11c (m) CD15 (h) CD19 (h, m) CD20 (h) CD45 (h, m) CD45RA (h) CD45R (m) GR-1 (m) HLA-DR (h) Reactivity: h=human and m=mouse Emission Spectra Comparison efluor 605 NC vs. Pacific Orange B220 efluor 605 NC B220 Pacific Orange CD3 Pacific Blue CD3 Pacific Blue efluor Nanocrystals have a narrow emission spectra compared to organic dyes. Although both efluor 605 NC and Pacific Orange will be excited by the violet laser, efluor 605 NC will be easier to compensate because of its narrow emission spectra. Comparison of efluor 605 NC and Pacific Orange staining. Mouse splenocytes were stained with Pacific Blue anti-mouse CD3 (17A2) and efluor 605 NC anti-mouse B220 (RA3-6B2) (left panel) or Pacific Orange anti-mouse B220 (RA3-6B2) (right panel). Total viable cells in the lymphocyte gate were used for analysis.
efluor Nanocrystals for flow cytometry for Flow Cytometry Catalog No. Description Clone Size Human Direct Conjugates 93-0048-71, 73 efluor 605 NC anti-human CD4 OKT4 25T, 100T 93-0458-71,73 efluor 605 NC anti-human CD45RA HI100 25T, 100T 93-0118-71, 73 efluor 605 NC anti-human CD11b ICRF44 25T, 100T 93-9956-71, 73 efluor 605 NC anti-human HLA-DR LN3 25T, 100T 93-0199-71, 73 efluor 605 NC anti-human CD19 HIB19 25T, 100T 93-0459-71,73 efluor 605 NC anti-human CD45 HI30 25T, 100T 94-0037-71, 73 efluor 625 NC anti-human CD3 OKT3 25T, 100T 94-0088-71, 73 efluor 625 NC anti-human CD8 RPA-T8 25T, 100T 94-0159-71, 73 efluor 625 NC anti-human CD15 HI98 25T, 100T 94-0209-71, 73 efluor 625 NC anti-human CD20 2H7 25T, 100T Mouse Direct Conjugates 93-0041-71, 73 efluor 605 NC anti-mouse CD4 GK1.5 25T, 100T 93-0451-71, 73 efluor 605 NC anti-mouse CD45 30-F11 25T, 100T 93-0112-71, 73 efluor 605 NC anti-mouse CD11b M1/70 25T, 100T 93-0452-71, 73 efluor 605 NC anti-mouse B220 RA3-6B2 25T, 100T 93-0081-71, 73 efluor 605 NC anti-mouse CD8 53-6.7 25T, 100T 94-5931-71, 73 efluor 625 NC anti-mouse GR-1 RB6-8C5 25T, 100T 94-0193-71, 73 efluor 625 NC anti-mouse CD19 1D3 25T, 100T 94-0114-71, 73 efluor 625 NC anti-mouse CD11c N418 25T, 100T Mouse Isotype Controls 93-4714-32, 94 efluor 605 NC Mouse IgG1 Isotype Control - 125µl, 500 µl 93-4724-32, 94 efluor 605 NC Mouse IgG2a Isotype Control - 125µl, 500 µl 93-4732-32, 94 efluor 605 NC Mouse IgG2b Isotype Control - 125µl, 500 µl 94-4714-32, 94 efluor 625 NC Mouse IgG1 Isotype Control - 125µl, 500 µl 94-4724-32, 94 efluor 625 NC Mouse IgG2a Isotype Control - 125µl, 500 µl 94-4732-32, 94 efluor 625 NC Mouse IgG2b Isotype Control - 125µl, 500 µl 94-4752-32, 94 efluor 625 NC Mouse IgM Isotype Control - 125µl, 500 µl Rat Isotype Controls 93-4321-32, 94 efluor 605 NC Rat IgG2a Isotype Control - 125µl, 500 µl 93-4031-32, 94 efluor 605 NC Rat IgG2b Isotype Control - 125µl, 500 µl 94-4321-32, 94 efluor 625 NC Rat IgG2a Isotype Control - 125µl, 500 µl 94-4031-32, 94 efluor 625 NC Rat IgG2b Isotype Control - 125µl, 500 µl Armenian Hamster IgG Isotype Control 94-4888-32, 94 efluor 625 NC Armenian Hamster IgG Isotype Control - 125 µl, 500 µl Streptavidin 93-4317-71, 73 efluor 605 NC streptavidin conjugate - 25T, 100T 94-4317-71, 73 efluor 625 NC streptavidin conjugate - 25T,100T
efluor Nanocrystals Introducing a NEW range of fluorochromes for Imaging and Microscopy efluor Nanocrystals ( NC ) are high quantum yield nanocrystals that possess a core particle surrounded by a patented surface coating to reduce background staining and artifacts. efluor Nanocrystals are easy to use. Their unique physical and spectral properties give them distinct advantages over organic dyes and make them excellent products for researchers performing multicolor applications. efluor nanocrystal products are named for their emission wavelength. Our rapidly growing portfolio is packaged to be economical and convenient. High photostability Narrow emission spectra compared to organic dyes Non-toxic efluor 700 NC cadmium-free InGaP nanocrystals in vivo imaging cell labeling Streptavidin efluor 605 NC for detection of biotin-labeled primary antibodies frozen or paraffin embedded tissue samples cultured cells efluor Nanocrystals for imaging & MICROSCOPY ebioscience New Releases October 2008 featuring 490 NC 525 NC 605 NC 625 NC 700 NC efluor nanocrystal products are designed for a broad range of applications including: Immunofluorescence Microscopy In vivo Imaging Flow Cytometry Tools for Discovery Immunofluorescence with efluor 605 NC efluor 700 NC for in vivo Imaging Frozen tissue section stained with Streptavidin efluor 605 NC Frozen sections of mouse lymph node were stained with either no primary antibody (left panel) or biotin anti-mouse CD3 (145-2C11) (right panel) and subsequently with streptavidin efluor 605 NC to visualize the T cell zone. FITC anti-mouse B220 (RA3-6B2) was used to identify B cell follicles. efluor Nanocrystals can be used to visualize solid tumor in vivo. Mice were injected with either PBS (left mouse) or with 100 pmol of efluor 700 NC (non-functionalized) (right mouse) into a subcutaneous tumor. efluor Nanocrystals can be used to image tissue in vivo. Mice were injected with either PBS (right mouse) or with 100 pmol of efluor 700 NC (non-functionalized) (left mouse) into the liver at a depth of 1.5 cm.
efluor Nanocrystals for imaging & Microscopy for Imaging and Microscopy Catalog No. Description Size 93-2317-93 efluor 605 NC streptavidin conjµgate (IF) 100 µl 90-6364-33 efluor 490 NC (Carboxyl) 200 µl 91-6364-33 efluor 525 NC (Carboxyl) 200 µl 93-6364-33 efluor 605 NC (Carboxyl) 200 µl 94-6364-33 efluor 625 NC (Carboxyl) 200 µl 96-6364-34 efluor 700 NC (Carboxyl) 250 µl 90-6366-33 efluor 490 NC (Amine) 200 µl 91-6366-33 efluor 525 NC (Amine) 200 µl 93-6366-33 efluor 605 NC (Amine) 200 µl 94-6366-33 efluor 625 NC (Amine) 200 µl 96-6366-34 efluor 700 NC (Amine) 250 µl 90-6374-33 efluor 490 NC (non-functionalized) 200 µl 93-6374-33 efluor 605 NC (non-functionalized) 200 µl 94-6374-33 efluor 625 NC (non-functionalized) 200 µl 96-6374-34 efluor 700 NC (non-functionalized) 250 µl Anti-Human (Biotin Format) 13-0108-80 CD10 (CALLA) 25 µg 13-0108-82 CD10 (CALLA) 100 µg 13-0159-80 CD15 25 µg 13-0159-82 CD15 100 µg 13-0209-80 CD20 25 µg 13-0209-82 CD20 100 µg 13-0849-80 CD84 25 µg 13-0849-82 CD84 100 µg 13-1449-80 CD144 25 µg 13-1449-82 CD144 100 µg 13-1957-80 CCR5 25 µg 13-1957-82 CCR5 100 µg 13-4776-80 Foxp3 25 µg 13-4776-82 Foxp3 100 µg 13-4777-80 Foxp3 25 µg 13-4777-82 Foxp3 100 µg 13-5889-80 CD275 (B7RP-1) 25 µg 13-5889-82 CD275 (B7RP-1) 100 µg 13-9996-80 Ig Lambda Light Chain 25 µg 13-9996-82 Ig Lambda Light Chain 100 µg 13-9997-80 Ig Kappa Light Chain 25 µg 13-9997-82 Ig Kappa Light Chain 100 µg Catalog No. Description Size Anti-Mouse (Biotin format) 13-0454-81 CD45.2 50 µg 13-0454-82 CD45.2 100 µg 13-0454-85 CD45.2 500µg 13-2012-80 CD201 (EPCR) 25 µg 13-2012-82 CD201 (EPCR) 100 µg 13-4013-85 IgG 500µg 13-5854-80 GITRL 25 µg 13-5854-82 GITRL 100 µg 13-9021-80 TLR2 25 µg 13-9021-82 TLR2 100 µg 36-0031-85 CD3e 500 µg 36-0041-85 CD4 (L3T4) 500 µg 36-0081-85 CD8a 500 µg 36-0112-85 CD11b 500 µg 36-5321-85 MHC Class II (I-A/I-E) 500 µg 36-5961-85 TCRb 500 µg 13-5773-80 Foxp3 25 µg 13-5773-82 Foxp3 100 µg Isotype Controls (Biotin Format) 13-4888-81 Armenian Hamster IgG 50 µg 13-4888-85 Armenian Hamster IgG 500 µg 13-4914-81 Golden Syrian Hamster IgG 50 µg 13-4914-85 Golden Syrian Hamster IgG 500 µg 13-4714-85 Mouse IgG1 500 µg 13-4724-85 Mouse IgG2a 500 µg 13-4732-85 Mouse IgG2b 500 µg 13-4301-81 Rat IgG1 50 µg 13-4301-85 Rat IgG1 500 µg 13-4321-81 Rat IgG2a 50 µg 13-4321-85 Rat IgG2a 500 µg 13-4031-81 Rat IgG2b 50 µg 13-4031-85 Rat IgG2b 500 µg