BioWhittaker specialty media Maximize your cell growth and productivity

Similar documents
BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity

BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity

4 Media and Reagents. Bioscience Solutions

Bioprocessing Media and Buffers Product Catalog

Media and Supplements

Take your research one step closer to in vivo

LONG R3IGF-I: An Insulin Alternative Exclusively for Cell Culture Applications

TRUST: Fisher is a name you can trust, with more than 100 years

Poietics Rat Mesenchymal Stem Cells Instructions for Use

Atlanta Biologicals Fetal Bovine Serum. and Cell Culture Media & Reagents

Thermo Scientific Metabolic Pathway Design Process Cell culture media and process optimization approaches for optimal biotherapeutic production

Creating RAFT 3D Cell Cultures with Different Thicknesses and Different Cell Types

CTS Cell. Therapy Systems. Products to help you move your research from the bench to the clinic

Amaxa Cell Line Nucleofector Kit L

Product Information.

PRIME-XV Cell Therapy Products by

THE ESSENTIALS OF LIFE SCIENCE RESEARCH GLOBALLY DELIVERED ATCC HIGH PERFORMANCE MEDIA AND SERA

cardiovascular cell Solutions

Guide to Clonetics and Poietics Cell Types

FectoCHO Expression system DNA transfection kit for protein production PROTOCOL

Amaxa 4D-Nucleofector Protocol for Undifferentiated Human Mesenchymal Stem Cells [MSC] For 4D-Nucleofector X Unit Transfection in suspension

atcc High Performance media and sera

BalanCD HEK293 SYSTEM

Potelligent CHOK1SV. How It Works. Potelligent CHOK1SV

TransIT -Insect Transfection Reagent

PRIME-XV Cell Therapy Products by

Abstract. Materials and Methods. Introduction

Cell Therapy Services Your Product. Our Passion.

BalanCD HEK293 SYSTEM

Product Size Catalog Number. 500,000 cryopreserved cells 500,000 proliferating cells 500,000 cryopreserved cells 500,000 proliferating cells

Amaxa 96-well Shuttle Protocol for Primary Cell Optimization

Poietics Human Mesenchymal Stem Cells

Amaxa 4D-Nucleofector Basic Protocol for Human Stem Cells For 4D-Nucleofector X Unit Transfection in suspension

CHOgro Expression System

PRIME-XV T CELL CDM. Chemically-defined, animal component-free medium for T cell culture

Amaxa Automated Nucleofection on Tecan Freedom EVO Workstation

TRANSFEX - SUPERIOR GENE EXPRESSION FOR HARD-TO-TRANSFECT CELL TYPES. Kevin Grady Product Line Business Manager ASCB Vendor Showcase Dec.

Mesenchymal Stem Cells

ClonaCell -CHO. Semi-Solid Cloning Testing Guidelines

CloneMedia and XP Media CHO Growth A Reagents Table 0-1: Available Reagents

Amaxa Cell Line 96-well Nucleofector Kit SF

PluriQ TM Serum Replacement (PluriQ TM SR)

SCIENTIFIC. Process for Success. CHO ONE Media System. Expression Media for Fed-Batch Culture

Clonetics Skeletal Muscle Myoblast Cell Systems HSMM Instructions for Use

Serum-Free, Xeno-Free Differentiation Media. for Human Mesenchymal Stem Cells

PROTOCOL PluriQ Serum Replacement

PRIME-XV Hematopoietic Cell Basal XSFM

Amaxa 4D-Nucleofector Protocol for Rat Hippocampal or Cortical Neurons For 4D-Nucleofector X Unit Transfection in suspension

ReproRNA -OKSGM is a non-integrating, self-replicating RNA-based reprogramming vector for generating induced pluripotent stem (ips)

Avalanche -Everyday Transfection Reagent

TransIT -293 Transfection Reagent

STEMdiff Mesenchymal Progenitor Kit

BD PureCoat ECM Mimetic Cultureware. Natural Performance. Scientifically Defined.

TransIT -Insect Transfection Reagent

Human Dermal Fibroblast Manual

Transfection Reagents That Really Work

GeneCellin TM HTC Transfection Reagent Protocol vs6 2/12

FectoPRO DNA transfection kit for Bioproduction PROTOCOL

Amaxa 96-well Shuttle Optimization Protocol for Cell Lines

Challenges in the cgmp Manufacturing of hescs: Lessons Learned from Monoclonal Antibodies

Avalanche -Omni Transfection Reagent

TransIT-PRO Transfection Kit Protocol for MIR 5700 and 5760

Avalanche -Omni Transfection Reagent

icell Hematopoietic Progenitor Cells Prototype User s Guide

STEMdiff Hematopoietic Kit

HiMesoXL TM Mesenchymal Stem Cell Expansion Medium

STANDARD OPERATING PROCEDURE

Amaxa Cell Line 96-well Nucleofector Kit SF

Human Bone Marrow Derived Mesenchymal Stem Cell (MSC) Care Manual

TransIT -Jurkat Transfection Reagent

Amaxa 96-well Shuttle Basic Protocol for Primary Mammalian Smooth Muscle Cells (SMC)

TransIT-PRO Transfection Kit Protocol for MIR 5700 and 5760

Product Size Catalog Number. 500,000 cryopreserved cells 500,000 proliferating cells 500,000 cryopreserved cells 500,000 proliferating cells

Lipodin Pro Protein Transfection Reagent

Clonetics Endothelial Cell Systems Instructions for Use

CARE MANUAL INSTRUCTIONAL MANUAL ZBM SHIPPING CONDITIONS STORAGE CONDITIONS ORDERING INFORMATION AND TECHNICAL SERVICES

All quality control test results are reported on a lot specific Certificate of Analysis which is available at or upon request.

INTESTINAL ORGANOIDS. IntestiCult and STEMdiff Media for Intestinal Organoid Culture. Scientists Helping Scientists

TurboFect in vitro Transfection Reagent. Description. Storage. Reagents to be Supplied by the User. #R ml ( for in vitro transfections)

Instructions For Research Use Only. Not For Use In Diagnostic Procedures. Rat Mesenchymal Stem Cell Starter Kit. Rat Mesenchymal Stem Cell Starter Kit

BD Resurge Media Supplements Chemically Defined. Protein Free. Animal Free.

Application Note. Introduction. Kerry Thompson, Jeff Partridge, Paula Flaherty, Susan Qian, and Deepa Saxena

Rat Mesenchymal Stem Cell Starter Kit

1. Serum-free Systems

Ready to use: Serum free culture in Insectomed SF express

FunctionMAX TiterEnhancer Overview. Customer name here Account Manager name here Date

TransIT-PRO Transfection Reagent Protocol for MIR 5740 and 5750

Amaxa Basic Neuron SCN Nucleofector Kit

Instructions For Research Use Only. Not For Use In Diagnostic Procedures. Rat Mesenchymal Stem Cell Starter Kit. Rat Mesenchymal Stem Cell Starter Kit

Avalanche -Everyday Transfection Reagent

All quality control test results are reported on a lot specific Certificate of Analysis which is available at or upon request.

Transfection Reagent INTRODUCTION SPECIFICATIONS MATERIALS. For Research Use Only. Materials Supplied. Materials required, but not supplied

Price Guide Advancing Research with Biologically Relevant Solutions

Mesenchymal Stem/Stromal Cells

Instructions For Research Use Only. Not For Use In Diagnostic Procedures. Rat Mesenchymal Stem Cells. Rat Mesenchymal Stem Cells.

HYPE-CHO Transfection Kit Results

BioResearch. RAFT 3D Cell Culture Kit Protocol

NutriVero Flex 10. Next-generation chemically defined serumfree, animal component free medium designed to support the growth of Vero cells

cgmp manufacture FDA-regulated ISO13485 certified CLASSICAL CELL CULTURE SOLUTIONS for high quality research results

ATCC ANIMAL CELL CULTURE GUIDE

Transcription:

BioResearch BioWhittaker specialty media Maximize your cell growth and productivity BioWhittaker specialty media cell culture applications

BioResearch BioWhittaker specialty media Maximize your cell growth and productivity If your work leads to downstream therapeutic, diagnostic, or biopharmaceutical applications, serum-free media is the best choice initiating your research studies. BioWhittaker specialty media deliver outstanding permance, a broad selection, and consistent results, whether you need protein-free, non-animal (NAO) or chemically media. Our ISO 9000 and cgmp systems are recognized industry wide. BioWhittaker serum-free media deliver unique benefits: Consistent results Elimination of FBS lot qualification Elimination of FBS-borne mycoplasma and virus contamination Simplified downstream purification Reduced regulatory burdens Maximal yields General purpose media UltraCULTURE serum-free medium Delivers excellent growth and expansion of a broad range of adherent and suspension cells Simplify your media needs, no FBS needed PC-1 chemically, serum-free medium Superior media primary adherent cells HL-1 chemically, serum-free medium Unique media hybridoma and lymphocytic cells UltraCULTURE medium p Most cell types PC-1 CD medium p p Adherent primary cells and cell lines HL-1 CD medium p Hybridomas and many adherent cell lines 12001-336 UltraCULTURE medium 500 ml 12001-774 PC-1 CD medium 2 X 500 ml 12001-772 HL-1 CD medium 2 X 500 ml 2

Mesenchymal stem cell medium MSCGM-CD chemically, serum-free MSC growth medium multiple passage expansion of all types of hmscs Cells can be directly transitioned from serum-containing medium with little to no adaptation time No need attachment matrix to plate cells Supports multi-lineage differentiation 95062-686 MSCGM-CD mesenchymal stem cell chemically growth medium BulletKit Kit 95062-688 MSCBM-CD mesenchymal stem cell chemically basal medium 500 ml 95062-690 MSCGM-CD mesenchymal stem cell chemically growth medium SingleQuots kit 5 ml Fibroblast medium FGM-CD chemically, serum-free fibroblast growth medium adult normal dermal fibroblasts and also supports neonatal cells Supports expression of fibroblast markers Ab5B5, CD90, and collagen I Collagen production is superior to that obtained with DMEM + FBS No weaning seamless transition from serum-containing media 71003-018 FGM-CD fibroblast growth medium chemically, BulletKit with L-glutamine, without phenol red or antibiotics Kit 71003-020 FBM-CD fibroblast basal medium chemically 500 ml 71003-022 FGM-CD fibroblast growth medium chemically, SingleQuots kit Kit Cryopreservation medium ProFreeze CDM (2X), protein-free freezing medium Formulated cryopreserving all cell types Maintains high cell viability upon recovery from frozen storage Requires 15% DMSO at 2X 12002-076 ProFreeze CDM (2X) 100 ml 3

BioResearch BioWhittaker specialty media Hematopoietic media X-VIVO chemically, serum-free hematopoietic cell media Superior media most hematopoietic cell types including dendritic cells, lymphocytes, monocytes, macrophages, and granulocytes Highly cited in scientific literature X-VIVO 10 medium p PBL, LAK, monocytes, macrophages, stem cells X-VIVO 15 medium p p PBL, NK, TIL, monocytes, macrophages, stem cells, dendritic cells X-VIVO 20 medium p PBL, LAK, TIL, monocytes, macrophages, stem cells, dendritic cells 12001-984 X-VIVO 10 with L-glutamine, gentamicin and phenol red 1 L 12002-004 X-VIVO 10 with L-glutamine, without gentamicin or phenol red 1 L 12002-006 X-VIVO 15 with L-glutamine, without gentamicin or phenol red 1 L 12001-990 X-VIVO 20 with L-glutamine, gentamicin and phenol red 1 L 12001-988 X-VIVO 15 with L-glutamine, gentamicin and phenol red 1 L PER.C6 medium ProPer 1 chemically, serum-free medium human embryonic retinoblast PER.C6 (and related) cells Recombinant protein and virus production ProPer 1 CD medium p p p PER.C6 (and related) cells in suspension 12002-174 ProPer 1 CD medium 1 L 4

CHO media PowerCHO chemically, serum-free CHO media New chemically PowerCHO media bring new levels of cell proliferation and protein production to chemically media Maintain high viability (>90%) at high cell densities UltraCHO serum-free CHO medium The al medium serum-free CHO use with suspension or adherent cells ProCHO protein-free CHO media Multiple mulas to optimize your protein-free applications including adherent and suspension cells, with high proliferation rates, and high protein yield Directly convert cultures from adherent with serum to suspension without serum PowerCHO and ProCHO IgG production ProCHO 4 supports concurrent transition to serum-free medium IgG (ng/ml) 2,500 2,000 1,500 1,000 500 Viable Cells /ml (x10 5 ) 14 12 10 8 6 4 2 0 ProCHO 4 PowerCHO 1 0 0 1 2 Passages PowerCHO 2 PowerCHO 3 ProCHO 4 Commercial supplier A Commercial supplier A Commercial supplier B Commercial supplier B Commercial supplier A + Insulin Commercial supplier C Commercial supplier D Commercial supplier B + Insulin CHO-K1 cells grown adherently in T-flasks using DMEM/F12 + 10% FBS were subcultured directly into 500 ml spinner flasks containing 200 ml of the respective medium. PowerCHO 1, 2, 3 CD media p p p Suspension CHO ProCHO 4, 5 media p p Suspension CHO ProCHO AT medium p p Adherent CHO UltraCHO medium p Adherent and suspension CHO 12002-186 PowerCHO 1 CD medium 1 L 12002-188 PowerCHO 2 CD medium 1 L 12002-190 PowerCHO 3 CD medium 1 L 12001-530 ProCHO 4 medium 1 L 12001-628 ProCHO 5 medium 1 L 12002-172 ProCHO AT medium 1 L 12001-610 UltraCHO medium 1 L 5

BioResearch BioWhittaker specialty media Renal media Pro293 chemically media Formulas both suspension and adherent culture ProVero 1 medium Supports Vero and MDCK in viral vaccine applications Pro293 s easy adaptation to suspension culture UltraMDCK chemically medium Supports MDCK in viral vaccine applications at both high and low densities Pro293 a supports adherent high-density cell growth Viable Cells /ml (x 10 5 ) 12 60,000 10 50,000 8 40,000 6 30,000 Viable Cells (RFU) 4 20,000 2 10,000 0 0 0 1 2 3 4 5 6 7 8 0 2 3 4 5 Days Pro293 s-cdm Commercial supplier A Pro293 a-cdm Commercial supplier B Days Pro293 s CD medium p p p HEK 293 suspension Pro293 a CD medium p p p HEK 293 adherent ProVero 1 medium p p Vero, MDCK UltraMDCK CD medium p p MDCK, Vero 12002-026 Pro293 s CD medium 1 L 12001-494 Pro293 a CD medium 1 L 12002-178 ProVero 1 medium 1 L 12001-340 UltraMDCK CD medium 1 L 6

Insect medium Insect-XPRESS protein-free insect cell medium Versatile medium shaker flask or stationary culture of SF9, SF21, High Five and Drosophila cells Insect-XPRESS medium p SF9, SF21, High Five and Drosophila cells 12001-620 Insect-XPRESS medium 500 ml 12001-622 Insect-XPRESS medium 1 L Hybridoma media UltraDOMA chemically, serum-free hybridoma medium Designed cultivation of murine, human and chimeric hybridomas in batch culture ProDoma serum-free hybridoma medium, protein-free media your hybridoma needs Scalable from small flasks to large-scale bioreactors UltraDOMA -PF chemically, protein-free hybridoma medium Based on the al hybridoma media murine, human, and chimeric cells in a completely medium with no peptides or tissue extracts UltraDOMA CD medium p p Murine, human, and chimeric cell lines UltraDOMA -PF CD medium p p p p Murine, human, and chimeric cell lines ProDoma 1 CD medium p p p Murine, rat, human, and chimeric cell lines ProDoma 3 medium p p Murine, rat, human, and chimeric cell lines 12001-608 UltraDOMA CD medium 500 ml 12001-618 UltraDOMA -PF CD medium 500 ml 12002-176 ProDoma 1 CD medium 1 L 12002-182 ProDoma 3 medium 1 L 7

www.vwr.com Contact inmation North America Scientific support: 800 521 0390 (toll free) scientific.support@lonza.com Lonza Cologne GmbH 50829 Cologne, Germany For research use only. Not use in diagnostic procedures. PER.C6 and Permexcis are trademarks of Crucell N.V. High Five is a trademark of Boyce Thompson Institute Plant Research Inc. Unless otherwise noted, all trademarks herein are marks of the Lonza Group or its affiliates. The inmation contained herein is believed to be correct and corresponds to the latest state of scientific and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or the results to be obtained from the use of such inmation and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. No statement is intended or should be construed as a recommendation to infringe any existing patent. Copyright 2012, Lonza Cologne GmbH. All rights reserved. BR-MxCllGrwth 09/11 CD-BR020 VWR Prices and product details are current when published; subject to change without notice. Certain products may be limited by federal, state, provincial, or local regulations. VWR makes no claims or warranties concerning sustainable/green products. Any claims concerning sustainable/green products are the sole claims of the manufacturer and not those of VWR International, LLC. All prices are in US dollars unless otherwise noted. Offers valid in US, void where prohibited by law or company policy, while supplies last. VWR, the VWR logo and variations on the egoing are registered ( ) or unregistered trademarks and service marks, of VWR International, LLC and its related companies. All other marks referenced are registered by their respective owner(s). Visit vwr.com to view our privacy policy, trademarks owners and additional disclaimers. 2012 VWR 0112 3M Lit. No. 12046