Universal Real-Time PCR Reagents.

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Jennifer Freeman
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1 Universal Real-Time PCR Reagents

Universal Real-Time PCR Reagents On any instrument. Under any conditions. At any time.

Our all-new line of universal real-time PCR reagents is the latest addition to Bio-Rad s fully integrated

Utilizing advanced enzyme engineering and buffering technologies, these innovative reagents work on any

2 Universal Real-Time PCR Reagents On any instrument. Under any conditions. At any time. In the Tube (FPO) Sso7d (FPO) Processivity (FPO) It s time to rethink what you know about PCR. Our all-new line of universal real-time PCR reagents is the latest addition to Bio-Rad s fully integrated portfolio of PCR solutions. Utilizing advanced enzyme engineering and buffering technologies, these innovative reagents work on any instrument and under all conditions. Read our article, which describes how Bio-Rad s patented technology can enhance your results. Promo (FPO) Enhanced Real-Time PCR Results with Advanced Enzyme Technology: A Publication Review (tap to view full article)

3 Start (replace with a different headline?) Start here by tapping on one of the selections below to guide you to the perfect product choice. Select by Product Select directly from our family of universal real-time PCR reagents. Selection Table Use the selection table to choose a product based on sample, target, or application type.

4 Select Your Product Tap on your product of choice from the selection below. Reagents Reagents

5 Selection Table Tap on your product of choice based on your sample, target,or application type. Samples Targets Applications Tap on Selection Below Plasmid Purified gdna and cdna 40 60% GC-rich regions bp amplicons Gene expression analysis Genotyping mirna, lncrna, sirna knockdown Mutation detection Pathogen detection Singleplex and duplex reactions itaq Universal Same as itaq universal supermixes and Blood spot Crude extraction Formalin-fixed, paraffin-embedded (FFPE) Laser capture microdissection (LCM) Plant extract Plant tissue Soil Same as itaq universal supermixes and >60% GC-rich regions <40% GC-rich regions Increased secondary structure Longer amplicons >250 bp Same as itaq universal supermixes and... Chromatin immunoprecipitation (ChIP) Copy number variation Gene expression low-copy detection Methylation studies Singleplex, duplex, and triplex reactions Viral/pathogen low-copy detection SsoAdvanced Universal

SsoAdvanced Universal Reagents itaq Universal Reagents Home SsoAdvanced universal SYBR Green supermix is the newest high-performance real-time PCR supermix based on Bio-Rad s patented* Sso7d fusion

6 SsoAdvanced Universal Reagents itaq Universal Reagents Home SsoAdvanced universal SYBR Green supermix is the newest high-performance real-time PCR supermix based on Bio-Rad s patented* Sso7d fusion protein technology, providing superior inhibitor tolerance, increased processivity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility. Product Performance Data Competitive Data Product Comparison Competitor Comparison Chart Ordering Information SsoAdvanced universal SYBR Green supermix lets you: Use any real-time PCR system proprietary blend of passive reference dyes enables ROX normalization of quantitative PCR (qpcr) data regardless of the ROX requirements Achieve superior results under various conditions broad range of cycling conditions, primer concentrations, and temperature ranges can be applied Obtain uncompromised results from challenging templates and targets data are produced from a wide variety of sample types, PCR inhibitors, and GC- or AT-rich target sequences with secondary structures Employ PrimePCR assays and panels predeveloped tools are validated to generate the highest quality of qpcr data without the need for optimization * U.S. patents 6,627,424; 7,541,170; and 7,560,260.

7 SsoAdvanced Universal Reagents itaq Universal Reagents Home SsoAdvanced universal probes supermix is the newest high-performance real-time PCR supermix based on Bio-Rad s patented* Sso7d fusion protein technology, enabling superior singleplex and duplex reactions without affecting PCR sensitivity, efficiency, or reproducibility in the presence of PCR inhibitors or under fast cycling conditions. Product Performance Data Competitive Data Product Comparison Competitor Comparison Chart Ordering Information SsoAdvanced universal probes supermix lets you: Carry out high-performance singleplex and duplex reactions robust performance in singleplex or duplex real-time PCR reactions enables increased data precision and saves samples, reagents, and time Use any real-time PCR system proprietary blend of passive reference dyes enables ROX normalization of quantitative PCR (qpcr) data regardless of the ROX requirements Achieve superior results under various conditions broad range of cycling conditions, primer concentrations, and temperature ranges can be applied Obtain uncompromised results from challenging templates and targets data are produced from a wide variety of sample types, PCR inhibitors, and GC- or AT-rich target sequences with secondary structures * U.S. patents 6,627,424; 7,541,170; and 7,560,260.

SsoAdvanced itaq Universal Reagents Universal Reagents Home Selection Grid Start Selection Table Videos Tutorials Resources itaq universal SYBR Green supermix is optimized for use on any real-time

8 SsoAdvanced itaq Universal Reagents Universal Reagents Home Selection Grid Start Selection Table Videos Tutorials Resources itaq universal SYBR Green supermix is optimized for use on any real-time PCR instrument regardless of ROX requirements, and utilizes our hot-start, antibody-mediated itaq DNA polymerase. Product Performance Data Competitive Data Product Comparison Competitor Comparison Chart Ordering Information itaq universal SYBR Green supermix lets you: Use any real-time PCR system proprietary blend of passive reference dyes enables ROX normalization of quantitative PCR (qpcr) data regardless of the ROX requirements Decrease run times and time to results without compromising qpcr data quality provides rapid polymerization kinetics and instant polymerase activation for run times less than 40 minutes Achieve superior results under various conditions broad range of cycling conditions, primer concentrations, temperature ranges, sample types, and target sequences can be applied

9 SsoAdvanced itaq Universal Reagents Universal Reagents Home Selection Grid Start Selection Table Videos Tutorials Resources itaq universal probes supermix enables highly quantitative PCR (qpcr) in singleplex or duplex reactions on any real-time PCR instrument regardless of ROX requirements. Product Performance Data Competitive Data Product Comparison Competitor Comparison Chart Ordering Information itaq universal probes supermix lets you: Carry out high-performance singleplex and duplex reactions robust performance in singleplex or duplex real-time PCR reactions enables increased data precision and saves samples, reagents, and time Decrease run times and time to results without compromising qpcr data quality provides rapid polymerization kinetics and instant polymerase activation for run times less than 40 minutes Achieve superior results under various conditions broad range of cycling conditions, primer concentrations, temperature ranges, sample types, PCR inhibitors, and target sequences can be applied

10 Videos Lab illorrorem corro officiur, ilia nimperum explant am, corume dolesequi aut dolorepra que veresec ullorum ent omnis eum aut re, non corio. Ceprati umquam, sus volecta ant aut eniae volupti onsectiae niminve llabo. Itat voluptati culpa quist, velitatia nis asperum qui quodis eumquate estis ipitis dolorae conet Thumbnail Promo Video 1 (Title TBD) Thumbnail Promo Video 2 (Title TBD) Thumbnail Promo Video 3 (Title TBD)

Tutorials These tutorials provide information about PCR and quantitative PCR (qpcr) processes and describe how the components in Bio-Rad s universal real-time PCR reagents function.

You will learn about the process of PCR and quantitative PCR (qpcr), including the different types of chemistry used SYBR Green and probes. You will also learn how to evaluate your data.

You will also learn about the various priming methods and which one is optimal for your experiments.

11 Tutorials These tutorials provide information about PCR and quantitative PCR (qpcr) processes and describe how the components in Bio-Rad s universal real-time PCR reagents function. Understanding Real-Time PCR This tutorial will introduce you to the interplay of the central dogma and PCR. You will learn about the process of PCR and quantitative PCR (qpcr), including the different types of chemistry used SYBR Green and probes. You will also learn how to evaluate your data. /qpcr_tutorial Understanding Reverse Transcription This tutorial will introduce you to how RNase H+ reverse transcriptases function and their advantages. You will also learn about the various priming methods and which one is optimal for your experiments. The second part of the tutorial covers experimental design through data analysis steps to help you perform a valid comparison between two reverse transcription kits. /rt_tutorial Understanding Real-Time PCR Supermixes This tutorial will introduce you to the different DNA polymerases, Taq and Sso7d-based novel enzymes, and the advanced buffering chemistry used to enable optimal real-time PCR data when faced with the most challenging sample types and target regions. The second part of the tutorial covers experimental design through data analysis steps to help you perform a valid comparison between two supermixes. /supermixes_tutorial

12 Resources These resources will help you select appropriate universal real-time PCR reagents and provide detailed instructions for their use. The glossary includes relevant terms used in these materials. Instruction Manuals Instruction Manual Part number Instruction Manual Part number Instruction Manual Part number Instruction Manual Part number Guides Real-Time PCR Applications Guide Bulletin 5279 Reagent Comparison Guide for Real-Time PCR Brochure Bulletin 6252 Product Brochures Amplification Reagents and Plastics Brochure Bulletin 6090 PrimePCR Assays and Panels Brochure Bulletin 6290 Glossary See Nogginslab tutorial for glossary.

13 Legal Cy is a trademark of GE Healthcare group companies. FAM, ROX, and StepOnePlus are trademarks of Applera Corporation. FastMix, PerfeCTa, and ToughMix are trademarks of Quanta BioSciences, Inc. GreenER, SYBR, and ViiA are trademarks of Life Technologies Corporation. Bio-Rad Laboratories, Inc. is licensed by Life Technologies Corporation to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only. QuantiFast and QuantiTect are trademarks of QIAGEN GmbH. TaqMan is a trademark of Roche Molecular Systems, Inc. Practice of the patented 5' Nuclease Process requires a license from Applied Biosystems. The purchase of these products includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser s own internal research when used with the separate purchase of Licensed Probe. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Practice of the polymerase chain reaction (PCR) may require a license. Bulletin 6346 Rev A

THUNDERBIRD SYBR qpcr Mix

Instruction manual THUNDERBIRD SYBR qpcr Mix 1304 A4251K THUNDERBIRD SYBR qpcr Mix QPS-201T 1 ml x 1 QPS-201 1.67 ml x 3 Contents [1] Introduction [2] Components [3] Primer design [4] Template DNA [5]