Introducing a new generation of affordable choices from the leader in real-time PCR. Applied Biosystems 7500 Fast Real-Time PCR System

Transcription

1 7300/7500 Real-Time PCR Systems Introducing a new generation of affordable choices from the leader in real-time PCR. Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR System Applied Biosystems 7300 Real-Time PCR System 7300/7500 Real-Time PCR Systems

2

3 RZ_ _Broschure_umschlag :20 Uhr Seite 2 A new generation of real-time PCR, built on a solid reputation for excellence. Real affordable. Real convenient. The Applied Biosystems 7300 Real-Time PCR System The whole genome. At your service and ready-to-use. Applied Biosystems TaqMan Genomic Assays are moving genetic discovery to the next level. These high-quality, biologically informative assays use both public and Celera genomic information to design optimal primer and probe sets based on our 5' nuclease chemistry, ideal for both quantitative gene expression and SNP genotyping applications. All TaqMan Genomic Assays are provided in a single-tube format and designed to run using universal cycling conditions that require no additional optimization. Since we pioneered real-time PCR nearly a decade ago, Applied Biosystems has continued to develop the technology to provide more powerful solutions to labs Our current offering of more than 600,000 TaqMan Gene Expression Assays is a comprehensive collection of predesigned primer and probe sets that enable researchers to quickly and easily perform quantitative gene expression studies on human, mouse, rat, Arabidopsis, and Drosophila genes. Additionally, our selection of over 1 million TaqMan SNP Genotyping Assays comprise the world s largest collection of biologically informative, validated, pre-designed assays available anywhere. of all sizes. The introduction of the Applied Biosystems 7500 Fast Real-Time PCR System represents the latest innovation in real-time technology fast thermal cycling in a standard 96-well format. The 7500 Fast system can provide real-time PCR results in about 35 minutes while seamlessly integrating into existing laboratory workflows. Applied Biosystems family of real-time platforms, which includes the Applied Biosystems 7300, 7500, 7500 Fast, and 7900HT Fast Real-Time PCR Systems, provide cutting-edge tools while making real-time PCR more accessible than ever. These systems are easy to use with next generation software that greatly simplifies your entire workflow and they re flexible, allowing you to run the real-time chemistry of your choice. And, of course, they re both backed by Applied Biosystems unmatched track record of quality and long-term reliability. Proven performance and superior data quality at a price any lab can afford. Applied Biosystems new 7300 Real-Time PCR System is a full-featured instrument that combines high-end results with exceptional budget sensitivity. The 3rd generation system is accurate, reliable, and easy to use. Advanced multicolor detection capabilities allow you to perform a wide variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and +/ assays that utilize internal positive controls with the highest confidence in your results. Precision optics and a charge-coupled device (CCD) camera, plus a sophisticated multi-componenting algorithm, assure superior accuracy, reproducibility, and reliability Optimized for use with standard dye sets: FAM /SYBR Green I, VIC /JOE, TAMRA, and ROX Latest generation Peltier-based thermal cycling system lets you use both standard 96-well plates and 0.2 ml tubes Compact instrument footprint fits in any laboratory Amplification plot shows log of change in normalized reporter fluorescence plotted vs. PCR cycle number. This plot from the 7300 system illustrates 9 logs of linear dynamic range for a TaqMan assay of plasmid DNA containing the hccnb1 target sequence in tenfold serial dilutions. (hccnb1 is human g2/mitotic specific cyclin b1, chromosome 5, 5q12.) To compliment our offering of off-the-shelf products, our custom TaqMan Assays are the best solution for customerspecific gene expression or genotyping assays. Simply submit your sequence, using a secure and confidential ordering mechanism and we will deliver a single tube format of either your gene expression or genotyping assay for any species. Results you can trust every time. Real-time PCR measures PCR amplification as it occurs, cycle-by-cycle, allowing quantitative measurements to be made in the highly reproducible exponential phase of PCR. This enables extremely accurate and precise With the Applied Biosystems 7300 system, you no longer need to sacrifice quality for affordability. This economical platform offers both proven reliability and superior data quality exactly what you would expect from the leader in real-time PCR systems. TaqMan Genomic Assays You can search for TaqMan Genomic Assays by gene symbol, public accession numbers, molecular function, or biological process. You can find more about TaqMan Gene Expression Assays at and more about TaqMan SNP Genotyping Assays at You ll also find useful data and valuable links to relevant references on both sites. quantitation over a large dynamic range compared to traditional techniques. In addition, the closed-tube format Multiplexed TaqMan assays on the 7300 system showing amplification of cdna (96 samples) using probes labeled with VIC (green) and FAM (black) reporters for the 18S and TGF -beta target sequences, respectively. minimizes the risk of contamination and eliminates all post-pcr processing. You get more precise and more accurate results with minimal hands-on time. Now you can take your real-time PCR as far as you need to go. To learn more about the new Applied Biosystems 7300 or 7500 Real-Time PCR Systems, call , or visit us on the Web at

4 RZ_ _Broschure_umschlag :20 Uhr Seite 2 A new generation of real-time PCR, built on a solid reputation for excellence. Real affordable. Real convenient. The Applied Biosystems 7300 Real-Time PCR System The whole genome. At your service and ready-to-use. Applied Biosystems TaqMan Genomic Assays are moving genetic discovery to the next level. These high-quality, biologically informative assays use both public and Celera genomic information to design optimal primer and probe sets based on our 5' nuclease chemistry, ideal for both quantitative gene expression and SNP genotyping applications. All TaqMan Genomic Assays are provided in a single-tube format and designed to run using universal cycling conditions that require no additional optimization. Since we pioneered real-time PCR nearly a decade ago, Applied Biosystems has continued to develop the technology to provide more powerful solutions to labs Our current offering of more than 600,000 TaqMan Gene Expression Assays is a comprehensive collection of predesigned primer and probe sets that enable researchers to quickly and easily perform quantitative gene expression studies on human, mouse, rat, Arabidopsis, and Drosophila genes. Additionally, our selection of over 1 million TaqMan SNP Genotyping Assays comprise the world s largest collection of biologically informative, validated, pre-designed assays available anywhere. of all sizes. The introduction of the Applied Biosystems 7500 Fast Real-Time PCR System represents the latest innovation in real-time technology fast thermal cycling in a standard 96-well format. The 7500 Fast system can provide real-time PCR results in about 35 minutes while seamlessly integrating into existing laboratory workflows. Applied Biosystems family of real-time platforms, which includes the Applied Biosystems 7300, 7500, 7500 Fast, and 7900HT Fast Real-Time PCR Systems, provide cutting-edge tools while making real-time PCR more accessible than ever. These systems are easy to use with next generation software that greatly simplifies your entire workflow and they re flexible, allowing you to run the real-time chemistry of your choice. And, of course, they re both backed by Applied Biosystems unmatched track record of quality and long-term reliability. Proven performance and superior data quality at a price any lab can afford. Applied Biosystems new 7300 Real-Time PCR System is a full-featured instrument that combines high-end results with exceptional budget sensitivity. The 3rd generation system is accurate, reliable, and easy to use. Advanced multicolor detection capabilities allow you to perform a wide variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and +/ assays that utilize internal positive controls with the highest confidence in your results. Precision optics and a charge-coupled device (CCD) camera, plus a sophisticated multi-componenting algorithm, assure superior accuracy, reproducibility, and reliability Optimized for use with standard dye sets: FAM /SYBR Green I, VIC /JOE, TAMRA, and ROX Latest generation Peltier-based thermal cycling system lets you use both standard 96-well plates and 0.2 ml tubes Compact instrument footprint fits in any laboratory Amplification plot shows log of change in normalized reporter fluorescence plotted vs. PCR cycle number. This plot from the 7300 system illustrates 9 logs of linear dynamic range for a TaqMan assay of plasmid DNA containing the hccnb1 target sequence in tenfold serial dilutions. (hccnb1 is human g2/mitotic specific cyclin b1, chromosome 5, 5q12.) To compliment our offering of off-the-shelf products, our custom TaqMan Assays are the best solution for customerspecific gene expression or genotyping assays. Simply submit your sequence, using a secure and confidential ordering mechanism and we will deliver a single tube format of either your gene expression or genotyping assay for any species. Results you can trust every time. Real-time PCR measures PCR amplification as it occurs, cycle-by-cycle, allowing quantitative measurements to be made in the highly reproducible exponential phase of PCR. This enables extremely accurate and precise With the Applied Biosystems 7300 system, you no longer need to sacrifice quality for affordability. This economical platform offers both proven reliability and superior data quality exactly what you would expect from the leader in real-time PCR systems. TaqMan Genomic Assays You can search for TaqMan Genomic Assays by gene symbol, public accession numbers, molecular function, or biological process. You can find more about TaqMan Gene Expression Assays at and more about TaqMan SNP Genotyping Assays at You ll also find useful data and valuable links to relevant references on both sites. quantitation over a large dynamic range compared to traditional techniques. In addition, the closed-tube format Multiplexed TaqMan assays on the 7300 system showing amplification of cdna (96 samples) using probes labeled with VIC (green) and FAM (black) reporters for the 18S and TGF -beta target sequences, respectively. minimizes the risk of contamination and eliminates all post-pcr processing. You get more precise and more accurate results with minimal hands-on time. Now you can take your real-time PCR as far as you need to go. To learn more about the new Applied Biosystems 7300 or 7500 Real-Time PCR Systems, call , or visit us on the Web at

5 Real fast and Real easy. The Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems 7500 Real-Time PCR System Maximum speed Real-Time PCR in 96-well format. The new Applied Biosystems 7500 Fast Real-Time PCR System takes you to the next level of speed in real-time PCR, enabling you to access your results more quickly than ever before. This integrated solution features three new components, a high-speed 96-well format thermal cycling block, TaqMan Fast Universal PCR Master Mix and Optical 96-Well Fast Thermal Cycling Plates. Quantitative real-time PCR results can be generated in under 40 minutes while the 96-well format allows seamless integration into existing laboratory workflows. Sophisticated and versatile platforms for users requiring extended capabilities, these third generation instruments feature an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The 7500 Fast Real-Time PCR System enables standard 96-well format high speed thermal cycling, significantly reducing your run time for quantitative real-time PCR applications, delivering results in under 40 minutes The 7500 Real-Time PCR System is a versatile, leading-edge platform providing enhanced performance capabilities and an upgrade path to high-speed thermal cycling Advanced optical configuration supports a broader range of fluorophores- including FAM /SYBR Green I, VIC /JOE, NED /TAMRA /Cy3, ROX /Texas Red, and Cy5 dyes; variable excitation capability allows greater sensitivity for longer wavelength (red) dyes Precision optics and charge-coupled device (CCD) camera, plus a sophisticated multi-componenting algorithm enable superior accuracy, reproducibility and reliability with no need for hardware changes to access new dyes An optional, service engineer installed upgrade kit is available for the 7500 System to convert it to the 7500 Fast System configuration Unmatched performance, results you can depend on. If you demand more from your real-time PCR instrument, Applied Biosystems 7500 Fast and 7500 systems are your new platforms of choice. It combines advanced features, powerful, and easy-to-use software as well as a proven reputation for reliability. Amplification plot from a 7500 Fast system shows results from a TaqMan Fast RNase P Instrument Verification Plate. Using this assay, the 7500 Fast platform can distinguish between samples containing 5,000 and 10,000 DNA template copies respectively, with a statistical confidence level of 99.7% and a run time of about 35 minutes.

6 Real intuitive. The Applied Biosystems 7300 and 7500 Real-Time PCR Systems also set a new standard for simplicity and usability. Both systems feature intuitive software that helps every user even beginners get expert results, quickly, and easily. For example, plate set-up wizards guide you through all aspects of experimental set-up for even complicated multiplex assays, including the use of multiple standard curves on a single plate. The real-time monitoring feature allows you to watch PCR amplification as it happens. Data analysis is simplified with built-in tools such as auto-baseline, auto-threshold, and SNP auto-calling. The RQ (Relative Quantitation) Study feature, (optional for the 7300 system) allows you to simultaneously analyze up to ten 96-well plates worth of gene expression data. Furthermore, advanced data viewing features allow you to visualize, report, and understand your datasets. Absolute Quantitation allows quantification of nucleic acid targets, including simultaneous analysis of multiple standard curves on the same plate RQ Study gene expression analysis software, with powerful data-viewing capabilities, for simultaneous analysis of up to ten 96-well plates Automated SNP genotype calling capability with intuitive graphical output and quality-value assignment Simple dissociation-curve data collection and viewing Real-time monitoring of amplification growth curves Auto-baseline and auto-threshold for simplified data analysis Tool tips for easy identification of sample wells when viewing amplification curve plots

7 Powerful new software speeds up and greatly simplifies experimental set-up, data viewing, and analysis even for non-experts. A SNP auto-caller automatically determines genotypes, and generates an intuitive graphic representation of results in a cluster plot report that helps you view data across populations or samples. Results are from Human CYP2C19*2 TaqMan SNP genotyping assay (using the 7300 system). Simple tab-driven formats allow you to move through experimental set-up with ease. The Instrument tab allows adjustment of thermal protocols including one-click addition of dissociation stages, easy editing of times, temperatures, and data collection steps. Dissociation curve view using SYBR Green I Dye assay chemistry. Relative Quantitation (RQ) Study gene expression analysis software with powerful data viewing capabilities allows simultaneous analysis of up to ten 96-well plates of data. Standard curve values showing C T values plotted vs. log of initial copy number. Amplification plot from the 7300 system illustrates 9 logs of linear dynamic range for a TaqMan assay of plasmid DNA containing the hccnb1 target sequence in tenfold serial dilutions. (hccnb1 is human g2/mitotic specific cyclin b1, chromosome 5, 5q12.)

8 Real versatile. The Applied Biosystems 7300 and 7500 Real-Time PCR Systems provide flexible dye detection. They are optimized for TaqMan probe-based and SYBR Green I Dye assay chemistries; however, both instruments also provide the features and capabilities to accommodate a wide range of other real-time chemistries. TaqMan probe-based assay chemistry provides outstanding specificity and sensitivity, and the ability to multiplex reactions for real-time quantitation and single nucleotide polymorphism (SNP) genotyping assays. The SYBR Green I Dye assay chemistry, while not as specific, provides an economical alternative for target identification (screening assays), or when only a small number of reactions are required for a given assay. Simplified assay development or no assay development at all! Both TaqMan probe-based and SYBR Green I Dye assay chemistries are supported by a comprehensive range of reagents and proven protocols that allow you to rapidly develop robust assays and eliminate assay optimization. But if you really want to save time, you can move your research ahead even faster with Applied Biosystems TaqMan Genomic Assays. Either way, universal thermal cycling conditions allow you to combine multiple assays in a single real-time run for unmatched flexibility. TaqMan Probe-Based Assay Chemistry SYBR Green I Dye Assay Chemistry 1. Polymerization: A fluorescent reporter (R) dye and a quencher (Q) are attached to the 5' and 3' ends of a TaqMan probe, respectively. 1. Reaction setup: The SYBR Green I dye fluoresces when bound to double-stranded DNA. FORWARD PRIMER PROBE REVERSE PRIMER 2. Denaturation: When the DNA is denatured, the SYBR Green I Dye is released and the fluorescence is drastically reduced. 2. Strand displacement: When the probe is intact, the reporter dye emission is quenched. 3. Polymerization: During extension, primers anneal and PCR product is generated. FORWARD PRIMER 3. Cleavage: During each extension cycle, the DNA polymerase cleaves the reporter dye from the probe. REVERSE PRIMER 4. Polymerization completed: Once separated from the quencher, the reporter dye emits its characteristic fluorescence. 4. Polymerization completed: When polymerization is complete, SYBR Green I Dye binds to the double-stranded product, resulting in a net increase in fluorescence detected by the 7300 and 7500 systems.

9 RZ_ _Broschure_umschlag :20 Uhr Seite 2 A new generation of real-time PCR, built on a solid reputation for excellence. Real affordable. Real convenient. The Applied Biosystems 7300 Real-Time PCR System The whole genome. At your service and ready-to-use. Applied Biosystems TaqMan Genomic Assays are moving genetic discovery to the next level. These high-quality, biologically informative assays use both public and Celera genomic information to design optimal primer and probe sets based on our 5' nuclease chemistry, ideal for both quantitative gene expression and SNP genotyping applications. All TaqMan Genomic Assays are provided in a single-tube format and designed to run using universal cycling conditions that require no additional optimization. Since we pioneered real-time PCR nearly a decade ago, Applied Biosystems has continued to develop the technology to provide more powerful solutions to labs Our current offering of more than 600,000 TaqMan Gene Expression Assays is a comprehensive collection of predesigned primer and probe sets that enable researchers to quickly and easily perform quantitative gene expression studies on human, mouse, rat, Arabidopsis, and Drosophila genes. Additionally, our selection of over 1 million TaqMan SNP Genotyping Assays comprise the world s largest collection of biologically informative, validated, pre-designed assays available anywhere. of all sizes. The introduction of the Applied Biosystems 7500 Fast Real-Time PCR System represents the latest innovation in real-time technology fast thermal cycling in a standard 96-well format. The 7500 Fast system can provide real-time PCR results in about 35 minutes while seamlessly integrating into existing laboratory workflows. Applied Biosystems family of real-time platforms, which includes the Applied Biosystems 7300, 7500, 7500 Fast, and 7900HT Fast Real-Time PCR Systems, provide cutting-edge tools while making real-time PCR more accessible than ever. These systems are easy to use with next generation software that greatly simplifies your entire workflow and they re flexible, allowing you to run the real-time chemistry of your choice. And, of course, they re both backed by Applied Biosystems unmatched track record of quality and long-term reliability. Proven performance and superior data quality at a price any lab can afford. Applied Biosystems new 7300 Real-Time PCR System is a full-featured instrument that combines high-end results with exceptional budget sensitivity. The 3rd generation system is accurate, reliable, and easy to use. Advanced multicolor detection capabilities allow you to perform a wide variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and +/ assays that utilize internal positive controls with the highest confidence in your results. Precision optics and a charge-coupled device (CCD) camera, plus a sophisticated multi-componenting algorithm, assure superior accuracy, reproducibility, and reliability Optimized for use with standard dye sets: FAM /SYBR Green I, VIC /JOE, TAMRA, and ROX Latest generation Peltier-based thermal cycling system lets you use both standard 96-well plates and 0.2 ml tubes Compact instrument footprint fits in any laboratory Amplification plot shows log of change in normalized reporter fluorescence plotted vs. PCR cycle number. This plot from the 7300 system illustrates 9 logs of linear dynamic range for a TaqMan assay of plasmid DNA containing the hccnb1 target sequence in tenfold serial dilutions. (hccnb1 is human g2/mitotic specific cyclin b1, chromosome 5, 5q12.) To compliment our offering of off-the-shelf products, our custom TaqMan Assays are the best solution for customerspecific gene expression or genotyping assays. Simply submit your sequence, using a secure and confidential ordering mechanism and we will deliver a single tube format of either your gene expression or genotyping assay for any species. Results you can trust every time. Real-time PCR measures PCR amplification as it occurs, cycle-by-cycle, allowing quantitative measurements to be made in the highly reproducible exponential phase of PCR. This enables extremely accurate and precise With the Applied Biosystems 7300 system, you no longer need to sacrifice quality for affordability. This economical platform offers both proven reliability and superior data quality exactly what you would expect from the leader in real-time PCR systems. TaqMan Genomic Assays You can search for TaqMan Genomic Assays by gene symbol, public accession numbers, molecular function, or biological process. You can find more about TaqMan Gene Expression Assays at and more about TaqMan SNP Genotyping Assays at You ll also find useful data and valuable links to relevant references on both sites. quantitation over a large dynamic range compared to traditional techniques. In addition, the closed-tube format Multiplexed TaqMan assays on the 7300 system showing amplification of cdna (96 samples) using probes labeled with VIC (green) and FAM (black) reporters for the 18S and TGF -beta target sequences, respectively. minimizes the risk of contamination and eliminates all post-pcr processing. You get more precise and more accurate results with minimal hands-on time. Now you can take your real-time PCR as far as you need to go. To learn more about the new Applied Biosystems 7300 or 7500 Real-Time PCR Systems, call , or visit us on the Web at

10 iscience. To better understand the complex interaction of biological systems, life scientists are developing revolutionary approaches to discovery that unite technology, informatics, and traditional laboratory research. In partnership with our customers, Applied Biosystems provides the innovative products, services, and knowledge resources that make this new, Integrated Science possible. Worldwide Sales Offices Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches 150 countries on six continents. For international office locations, please call the division headquarters or refer to our Web site at Applera is committed to providing the world s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Headquarters 850 Lincoln Centre Drive Foster City, CA USA Phone: Toll Free: Fax: For Research Use Only. Not for use in diagnostic procedures. Authorized Thermal Cycler This instrument in combination with its immediately attached sample block modules, comprise an instrument that is an Authorized Thermal Cycler. The purchase price of this Base Unit includes the up-front fee component of a license under the non-u.s. counterparts of U.S. Patents Nos. 4,683,202, 4,683,195 and 4,965,188 owned by F. Hoffmann-La Roche Ltd. (Roche) covering the Polymerase Chain Reaction (PCR) process to practice the PCR process for internal research and development using this instrument. The running royalty component of that license may be purchased from Applied Biosystems or obtained by purchasing Authorized Reagents. This instrument is also an Authorized Thermal Cycler for use with applications licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Purchase of this product does not itself convey to the purchaser a complete license or right to perform the PCR process. Further information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California or Roche Molecular Systems, Inc Atlantic Avenue, Alameda, California DISCLAIMER OF LICENSE: No rights for any application, including any in vitro diagnostic application, are conveyed expressly, by implication or by estoppel under any patent or patent applications claiming homogeneous or real-time detection methods, including patents covering such methods used in conjunction with the PCR process or other amplification processes. The 5' nuclease detection assay and certain other homogeneous or real-time amplification and detection methods are covered by United States Patent Nos. 5,210,015, 5,487,972, 5,804,375 and 5,994,056, owned by Roche Molecular Systems, Inc.; by corresponding patents and patent applications outside the United States, owned by F. Hoffmann-La Roche Ltd; and by United States Patent Nos. 5,538,848 and 6,030,787, and corresponding patents and patent applications outside the United States, owned by Applera Corporation. Purchase of this instrument conveys no license or right under the foregoing patents. Use of these and other patented processes in conjunction with the PCR process requires a license. For information on obtaining licenses, contact the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, or The Licensing Department, Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California, 94501, USA. Applied Biosystems and VIC are registered trademarks and AB (Design), iscience, iscience (Design), JOE, NED, ROX, FAM, and TAMRA are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. Cy3 and Cy5 are registered trademarks of Amersham Biosciences. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Dell is a trademark of Dell Corporation. Microsoft and Windows XP are registered trademarks of Microsoft Corporation. Pentium is a registered trademark of Intel Corporation. SYBR and Texas Red are registered trademark of Molecular Probes, Inc. All other trademarks are the property of their respective owners. Information is subject to change without notice Applied Biosystems. All rights reserved. Printed in the USA, 05/05. Publication 117BR05-05