18 th WAVLD Congress Development and Validation of tuberculosis real-time PCR kit

Transcription

1 18 th WAVLD Congress Development and Validation of tuberculosis real-time PCR kit MOINE Sandrine The world leader in serving science

2 Content Bovine tuberculosis overview Why a new tuberculosis PCR kit? Characteristic of product Performance of product Field evaluation Conclusion 2

3 Bovine tuberculosis About bovine tuberculosis Notifiable disease according to OIE Highly contagious disease impairing cattle industry worldwide Dairy Breeding Trade Beef Infects all mammalians Caused by Mycobacteria belonging to the tuberculosis complex (M. tuberculosis, M. bovis, M. africanum, M. microti, M. canetti, M. caprae, and M. pinnipedii) No treatment beside BCG vaccination Transmission: Significant zoonosis Direct contact between cattle or wildlife vs cattle Indirect contact (soil, transport of animals) 3

4 A new tuberculosis PCR Why? The Applied Biosystems VetMAX M. tuberculosis Complex PCR kit (ref: MTBC) will be used as a confirmatory test to determine the TB status of a suspicious animal. The kit completes the tuberculosis solution portfolio for Thermo Fisher Scientific. The MTBC kit is an improved version of the current VetMAX Mycobacterium tuberculosis complex PCR kit (TUB2W). Duplex, single well instead of a double well for customer convenience and cost optimization Ready-to-use master mix Multiple species approach Increased robustness against inhibitors Increased sensitivity by having an excellent specificity Detects all TB strains belonging to the MTBC including M.bovis, M.tuberculosis, M.microti, M.caprae, M.pinnipedi, M.africanum, M.canetti 4

5 Characteristics of MTBC kit PRODUCT FORMAT TECHNOLOGY DETECTION AMPLIFICATION programs ANALYSIS MATRICES SPECIES VetMAX M.Tuberculosis Complex Kit Ref: MTBC 100 tests Real time PCR Technology TaqMan probes DNA Duplex One well reaction Ready-to use MMx IS6110 repeat region Standard mode 50 C 2min 95 C 10min 95 C 15sec 60 C 1min With passive reference ROX Lymph nodes and surrounding tissues Bovine and wildlife (wild boar, badger, cervid) Fast mode 50 C 2min 95 C 10min 95 C 3sec 60 C 30sec 5

6 Complete workflow Samples Nucleic acid extraction Amplification Individual sample : Lymph nodes Surrounding tissues Automated purification system : Thermo Scientific TM KingFisher TM ml or Flex Magnetic Particle instruments Magnetic bead based kit: MagVet Universal Isolation kit PCR instruments : Applied Biosystems 7500, 7500 Fast and QuantStudio TM 5 Real-Time PCR System Real-time PCR kit : VetMAX M. tuberculosis Complex Single real-time PCR Duplex: Tb & Internal positive control Ready-to-use master mix ~3 hrs 6

7 Performances of MTBC kit Analytical Specificity Specific detection of MTBC Efficiency Around 100% Detection limit of PCR (LD PCR ) 16 copies / PCR (standard mode) 25 copies / PCR (fast mode) Repeatability CV < 1.55 % (standard mode) CV < 0.92 % (fast mode) Intermediate precision CV < 1.52 % (standard mode) CV < 1.15 % (fast mode) Robustness Stability Detection limit of Method (LD METHOD ) Unaffected by variable parameters tested (Volume of DNA / Volume of PCR mix / Time of hybridization / T C of hybridization) Validated on 15 months MagVet Universal isolation kit: 1 UG/µL of spiked sample (Lymphnodes) QIAamp DNA Mini kit: 2 UG/µL of spiked sample (Lymphnodes) 7

8 Field evaluation Field evaluation French partners 682 field samples tested (bovine, badger; wild boar) 2 samples preparations compared (with or without decontamination) Comparison to culture method Global results (Both sample preparation) New product (MTBC) Old product (TUB2W) Detected Not detected TOTAL Detected Not detected TOTAL Kappa correlation coefficient : 0.96 Diagnostic sensitivity: 97.5 % [ %] Diagnostic specificity: 98.4 % [ %] 8

9 Field evaluation Comparison between both PCR products H2SO4/NaOH decontamination New product (MTBC) Old product (TUB2W) Detected Not detected TOTAL Detected Not detected TOTAL Kappa correlation coefficient : 0.94 Diagnostic sensitivity: 95.4 % [ %] Diagnostic specificity: 98.3 % [ %] No decontamination (PBS1X Method) New product (MTBC) Old product (TUB2W) Detected Not detected TOTAL Detected Not detected TOTAL Kappa correlation coefficient : 1.0 Diagnostic sensitivity: % [ %] Diagnostic specificity: Not enough sample 9

10 Field evaluation Comparison to culture H2SO4/NaOH decontamination New product (MTBC) Culture Detected Not detected TOTAL Detected ** 138 Not detected 4* TOTAL Diagnostic sensitivity: 97.0 % [ %] Diagnostic specificity: 91.3 % [ %] * Results confirmed by French National Reference Lab (ANSES) ** Results confirmed by VetMAX Mycobacterium tuberculosis complex (TUB2W) No decontamination (PBS1X Method) New product (MTBC) Culture Detected Not detected TOTAL Detected * 123 Not detected TOTAL Diagnostic sensitivity: 99.1 % [ %] Diagnostic specificity: Not enough sample * Results confirmed by VetMAX Mycobacterium tuberculosis complex (TUB2W) 10

11 World evaluation in progress UK; APHA, New Haw Addlestone, Surrey KT15 3NB, UK Testing on lymph node and surrounding tissues France; Laboratoire Départemental D`Analyses Agriculture et Vétérinaire France (LVD24): Testing on lymph node and surrounding tissues Philippines; INFINNOMED ENTERPRISE's Quezon City; Manila Philippines Testing on lymph node and milk Uruguay; DILAVE, Montevideo Testing on lymph node and surrounding tissues New Zealand; Farina Khan Laboratory Manager (TB Diagnostics) Infectious Diseases, Animal Science, Agriresearch Testing on lymph node and surrounding tissues 11

12 CONCLUSION Bovine Tuberculosis is a re-emerging disease in many developed countries (UK, France, Germany, NZ). Bovine Tuberculosis becomes priority on all One Health approaches. VetMAX M. tuberculosis Complex PCR kit is a reliable diagnostic tool to confirm suspect cases of TB infected animals of different species - derived from mycobacteria belonging to the MTB Complex. The duplex single well approach is customer convenient in terms of number of tests per plate and analysis speed. MTBC complex detection including 7 pathogenic Mycobacteria Easy test interpretation Time to result improvement (compared to culture) diag tool adapted to the herds monitoring Product: Registered in France Available in the rest of the world in countries without registration plan 12

13 Acknowledgements French National Reference Lab (ANSES Maison Alfort) French Partner labs Laboratoire Départemental d analyses et de recherche de Dordogne Laboratoire départemental de Côte-d Or Laboratoire des Pyrénées et des Landes Other partner labs APHA, New Haw Addlestone, Surrey KT15 3NB - UK DILAVE, Montevideo - Uruguay INFINNOMED ENTERPRISE's Quezon City; Manila Philippines Farina Khan Laboratory Manager (TB Diagnostics) Infectious Diseases, Animal Science, Agriresearch New Zealand 13

14 For Veterinary Use Only. For In Vitro Use Only. Regulatory requirements vary by country; products may not be available in your geographic area Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. QIAamp is a trademark of Qiagen ltd 14