MAXimum results. Minimum effort.

Transcription

1 MAXimum results. Minimum effort. MagMAX sample preparation for veterinary diagnostic testing Sometimes purifying nucleic acid from animal samples can be like trying to pull a needle out of a haystack. Fortunately, the magnetic particle technology of the Applied Biosystems MagMAX sample preparation system makes purification convenient, flexible and efficient enabling maximum results with minimum effort. The MagMAX sample preparation system offers the following benefits: Efficiency Fast and easy sample processing typically less than 30 min run time for 96 samples* Process up to 800 samples per day on a single instrument* Higher sensitivity with less sample input Convenience and flexibility One system purifies both DNA and RNA from a wide variety of sample matrices Less hands-on manipulation There are Thermo Scientific KingFisher and Applied Biosystems MagMAX Express magnetic particle instruments available for any budget, application and throughput needed Reliability Less risk of cross-contamination Fewer false negatives due to effective removal of PCR inhibitors Helps improve the consistency of nucleic acid extraction yields *Depending on sample type

2 MagMAX technology The MagMax sample preparation system is an integrated solution that accelerates, standardizes and automates the extraction of nucleic acid to prepare for downstream molecular analysis. Consisting of Applied Biosystems MagMAX nucleic acid isolation kits along with KingFisher or MagMAX Express magnetic particle processors, the MagMAX sample preparation system is designed to provide an integrated solution that offers unparalleled ease of use, shorter time-to-results and high-quality nucleic acid extraction. The MagMAX sample preparation system can handle the most difficult challenges faced by veterinary diagnostic labs when extracting nucleic acid from biological fluids and cell-free sample types, such as low concentrations of extractable nucleic acid, large processing volumes and sample complexity. And this is done without using organic solvents or nucleic acid precipitation, thus minimizing the challenges often encountered with filterbased methods (e.g., clogging, large elution volumes and inconsistent yields). The MagMAX sample preparation system offers all of this, while minimizing the risk of cross-contamination. Convenience and flexibility Wide variety of sample matrices: Linear recovery of synthetic viral RNA from biological fluids. MagMAX Lysis Buffer was added to samples of water, plasma, serum and milk, and the lysate was then spiked with a serial dilution of a 466 nt HIV Armored RNA transcript. Viral RNA was isolated using the MagMAX Viral RNA Isolation Kit. The recovered RNA was analyzed by qrt-pcr. Reliability Greater sensitivity than competitors kits Efficiency MagMAX achieves equal sensitivity with 10x less sample than competitor s kit. Clinical tracheal and cloacal swab samples were collected from poultry infected with California exotic Newcastle disease (END). RNA was isolated from 50 μl of sample using the MagMAX protocol or 500 μl of samples using the NVSL validated protocol. Performance MagMax Kit vs. Competitor s kits. The MagMAX Pathogen RNA/DNA Kit outperforms competitor magnetic bead based nucleic acid purification kits. A high-titer PRRSV-positive serum sample was diluted to create mid-titer and low-titer samples. RNA was purified from each dilution in triplicate following each supplier s directions and using instrument scripts provided by each supplier. Purified RNA was analyzed by qrt-pcr using PRRSV primers and probes. Lowest C t values were obtained with RNA purified using the MagMAX Pathogen RNA/DNA Kit.

3 MagMAX nucleic acid isolation kits Years of research and development have produced a portfolio of nucleic acid isolation kits optimized to purify RNA and DNA from viruses, select bacteria and parasites found in sample matrices commonly submitted for veterinary diagnostic testing. The technology at the core of the MagMAX nucleic acid isolation kits: Binds RNA and DNA more efficiently than silicamembrane methods Offers higher and more consistent nucleic acid yields Allows nucleic acids to be eluted in as little as 20 μl for manual extraction and in as little as 25 μl for automated extraction of nuclease-free water, resulting in more concentrated RNA and DNA As a result, there is a MagMAX nucleic acid isolation kit to meet any lab s unique needs, including: KingFisher and MagMAX Express Magnetic Particle Processors The family of KingFisher and MagMAX Express Magnetic Particle Processors are ideal for those veterinary diagnostic and research labs looking to automate their MagMAX sample preparation system. For decades, KingFisher technology has been at the core of the industry s best magnetic particle processors. Our proprietary magnetic separation technology lets labs process virtually any sample from any source, while minimizing the risk of crosscontamination. In short, KingFisher magnetic particle processors offer superior results in nucleic acid isolation with: Effective bead collection and transfer, which leads to superior washing, elution efficiency and rapid processing Preprogrammed scripts optimized for each MagMAX kit and application A broad portfolio of instruments for any budget, application and throughput need The family of KingFisher and MagMAX Express magnetic particle processors includes: MagMAX Pathogen RNA/DNA Kit MagMAX Pathogen RNA/DNA Kit The most versatile solution for veterinary diagnostic labs, providing high-throughput extraction of pathogen RNA or DNA from 50 μl to 300 μl input. MagMAX-96 Viral RNA Isolation Kit Offers high-throughput extraction of pathogen RNA or DNA from 50 μl input. MagMAX-96 AI/ND Viral RNA Isolation Kit Validated extraction of AIV and NDV RNA for NVSL network labs. MagMAX Total Nucleic Acid Isolation Kit Provides effective extraction of DNA from tough-to-lyse bacteria from feces that requires bead beating. KingFisher Flex Processor Offering high-throughput or large processing volumes, the KingFisher Flex processor offers truly versatile nucleic acid purification. Two separate magnetic heads provide 96- or 24- well formats and processing volumes ranging between 20 μl to 5,000 μl. KingFisher Duo Prime Processor The newest KingFisher instrument combines advanced functionality, mid-throughput capacity (up to 24 amples), and supports large processing volumes (up to 5 ml).* KingFisher ml Processor Provides easy operation of up to 15 samples, including higher processing samples (50 1,000 μl).* MagMAX Express Processor The original KingFisher system purifies up to 24 samples ( μl) at lower processing volumes. MagMAX Viral RNA Isolation Kit Used in high-volume, low-throughput applications, utilizing 1.5 ml tubes with a magnetic rack. Other MagMAX kits are available from Thermo Fisher Scientific that may meet needs for laboratory applications outside of those described. For more information please go to thermofisher.com

4 The MagMAX Pathogen RNA/DNA Kit Workflow The MagMAX Pathogen RNA/DNA Kit A single kit that can accomodate: Purification of RNA and DNA Purification from viruses, bacteria and parasites Low and high sample input volumes A wide range of sample types Workflow Purify pathogen RNA/DNA Semen Feces InPouch Oral fluids Add Lysis Vortex Spin Blood Serum Plasma Swabs Milk Ear notches MagMAX Pathogen RNA/DNA Kit KingFisher & MagMAX Express Magnetic Particle Processors Tissue Mosquitos Add Lysis Disrupt Spin Sample type Sample preparation Prepare reactions VetMAX Reagents Primers and probes diluted, premixed and optimized for efficient nucleic acid amplification Internal positive control to monitor nucleic acid purification efficacy and PCR inhibition Quality reagents manufactured under ISO 9001 QMS conditions, providing confidence in the supply and quality of products Master mixes Amplifies in the presence of challenging PCR inhibitors Optimized for low copy number amplification of RNA and DNA targets Efficient amplification over 7 logs of RNA and DNA input amount Saves time and money by amplifying up to 4 different RNA targets simultaneously (multiplex kit, assay-dependent) Amplify and analyze Applied Biosystems 7500 Fast Real-Time PCR Systems Faster ramp rates yield rapid results without compromising assay quality Allows for more runs per day, with excellent performance equivalent to the 7500 system Applied Biosystems 7500 Fast Real-Time PCR System

5 Ordering information Product Quantity Cat. No. MagMAX kits MagMAX Pathogen RNA/DNA Kit 480 or 5 x 96 rxns MagMAX -96 Viral RNA Isolation Kit 96 rxns AM1836 MagMAX Total Nucleic Acid Isolation Kit 100 rxns AM1840 MagMAX -96 AI/ND Viral RNA Isolation Kit 384 rxns AM1835 MagMAX Viral RNA Isolation Kit 50 rxns AM1939 MagMAX -96 Viral RNA Isolation Kit 5 x 96 rxns AMB KingFisher instruments and consumables KingFisher Flex Magnetic Particle Processor with 96 Deep-Well Head 1 instrument KingFisher Flex Magnetic Particle Processor with 24 Deep-Well Head 1 instrument KingFisher Duo Prime Magnetic Particle Processor 1 instrument KingFisher ml Unit 1 instrument KingFisher Flex 96 Deep-Well Magnetic Head 1 head KingFisher Flex 24 Deep-Well Magnetic Head 1 head KingFisher Flex 24 Deep-Well Tip Combs and Plates 50 combs/plates KingFisher Flex 24 Deep-Well Plates 50 plates KingFisher 96 Tip Comb for KF Magnets 100 combs ** KingFisher 96 KF Microplates (200uL) 48 plates ** KingFisher Flex Microtiter Deep-Well 96 Plates 50 plates ** 12-Tip Combs for KingFisher Duo Prime 50 combs Tip Combs for KingFisher Duo Prime 48 combs Elution Strips for KingFisher Duo Prime 40 strips Combi Pack for KingFisher Duo Prime (tip combs, plates and elution strips) Pack (96 samples) KingFisher ml Tip Combs 800 combs KingFisher ml Tubes 900 tubes KingFisher ml Combi Pack (tip combs and tubes) Pack (240 samples) MagMAX Express instruments and consumables MagMAX Express Magnetic Particle Processor 1 instrument MagMAX Express-96 Standard Magnetic Particle Processor 1 instrument MagMAX Express-96 Deep Well Magnetic Particle Processor 1 instrument MagMAX Express Tip Combs 50 combs MagMAX Express Plates 50 plates MagMAX Express-96 Standard Tip Combs 100 combs MagMAX Express-96 Standard Plates 48 plates * MagMAX Express-96 Standard Magnetic Head 1 instrument MagMAX Express-96 Deep Well Tip Combs 100 combs * MagMAX Express-96 Deep Well Plates 50 plates * MagMAX Express-96 Deep Well Magnetic Head 1 instrument

6 Ordering information Product Quantity Cat. No. Additional sample prep products MagMAX Lysis/Binding Solution Concentrate 100 ml AM8500 MagMAX Wash Solution 1 Concentrate 205 ml AM8504 MagMAX Wash Solution 2 Concentrate 200 ml AM8640 Carrier RNA (1 mg/ml) 500 µl RNA Rapid Extraction Solution rxns AM9775 Magnetic-Ring Stand 1 each AM10050 Magnetic Stand-96 (24 pst magnets) 1 each AM10027 Single Place Magnetic Stand 1 each AM10026 * Consumables are compatible with KingFisher Flex deep-well unit ** Consumables are identical to comparable Applied Biosystems MagMAX Express consumables Find out more at thermofisher.com/animalhealth For Veterinary Use Only. For In Vitro Use Only. Regulatory requirements vary by country; products may not be available in your geographic area Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CO