Activities and role of the European Union Reference Laboratory for L. monocytogenes
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1 Activities and role of the European Union Reference Laboratory for L. monocytogenes Adrien ASSERE Deputy manager EURLs L. monocytogenes, Staphylococci, Milk & Milk Products ANSES-Laboratory for Food Safety, Maisons-Alfort (FR) EURL Lm
2 ANSES French Agency for Food, Environmental and Occupational Health & Safety 3 divisions 1350 staff 800 experts Regulated Products Science for Experts Appraisal Research and Reference 11 labs 9 EURLs 66 NRLs 19 Sept
3 ANSES Laboratory for food safety Boulogne-sur-Mer Fishery and aquaculture products 3 EURLs including Lm Maisons-Alfort Food safety 11 Laboratories / 16 sites 3
4 Role of reference laboratories Development and validation of methods Networking and training of official labs Inter-laboratory proficiency tests (ILPT) Scientific & technical assistance Data compilation & synthesis EURL NRL Official Labs ILPT Technology transfer Laboratory approval Scientific watch Foodborne outbreak investigation Agri-food Industry Labs own-checks Primary production Epidemiological surveillance (Animal health, Plant health, etc.) 19 Sept
5 EURL/NRL Lm network Since 2006 EC Reg 882/2004 mod. 776/ NRLs + FYROM and Norway At least 1 NRL from each EU Member State 19 Sept
6 EURL for Listeria monocytogenes ANSES-Lab for Food Safety - designated in
7 Legal frame Reg. EC 2073/2005 on microbiological criteria for foodstuffs Lm: 3 food safety criteria for ready-to-eat (RTE) foods RTE for infants & special medical purpose RTE able/unable to support Lm growth 2 types of limits Qualitative: absence/25 g Quantitative: 100 cfu/g at the end of shelf-life 19 Sept
8 Annual workshops Coordination of Lm network General scope or thematic Regulatory/epidemiological context (EFSA/ECDC) Works undertaken since last workshop Work programme for next year 19 Sept
9 @EURL or in situ Training courses Upon needs expressed by NRLs & for NRLs with unsatisfactory results to PTs Examples detection/enumeration PFGE typing + data analysis coorganised with EURLs VTEC & Salmo (EFSA-ECDC DB) Shelf-life studies Online video tutorials Lm PFGE typing profile interpretation 19 Sept
10 Assessment of NRLs performance Proficiency tests (PT) 1/year for regulatory parameters (Lm detection or enumeration with EN ISO or 2) Use of reference methods required PFGE (testing & interpretation), serotyping, MLST challenge test (Lm growth potential) Follow-up of corrective measures New test materials Matrices Level of contamination Contamination techniques 19 Sept
11 Assistance to NRLs Confirmatory analyses Support to NRLs Lm typing for investigating national outbreaks Support for National typing database implementation Support for organisation of national PTs Replies to queries ( , phone) Provide set of strains Participation to National network meetings 19 Sept
12 Analytical development EURL in-house work, possibly in collaboration with some NRLs To develop/improve & validate reference methods/reference materials for official control Impact of test portion/new Listeria species/sample pooling Characterization/typing methods (PFGE, faflp, MLVA, WGS) Certified reference materials in collaboration with EC/JRC (Geel) DNA-plugs for Lm PFGE typing Upon the needs 19 Sept
13 Networking = Collaboration+Harmonisation Drafting reference documents EFSA SOP on PFGE for Lm Guides for NRLs/national official labs/competent authorities Criteria for outsourcing part of PTs on Lm detection/enumeration Measurement uncertainty/lm enumeration Sampling techniques of surfaces of food processing environment for Lm analysis Shelf-life studies: challenge tests, durability study Lab competence to conduct shelf-life studies for CAs & NRLs 19 Sept
14 Support to DG SANTE WG on microbiological criteria Participation for DG SANTE to European/international technical meetings/activities CEN/ISO standardization (Lm detection/enumeration, challenge tests, WGS) Involvement in EC/third countries sanitary negotiations Participation to EFSA WGs Curation of EFSA/ECDC database for PFGE profiles of Lm food strains Participation to EURLs WG on WGS Investigation of European-level food-borne outbreaks (Lm) 19 Sept
15 Conclusion A virtuous circle Assistance to DG SANTE (EFSA-ECDC- JRC) Analytical development + Harmonisation of practices Support to NRLs Training courses Lab assessment 19 Sept
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