BioSep- SEC-S. Tired of paying high prices? Irritated with slow delivery times? Frustrated by irreproducible results?

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1 BioSep- SEC-S HPLC Columns for Gel Filtration / Size Exclusion Chromatography Tired of paying high prices? Irritated with slow delivery times? Frustrated by irreproducible results? Eliminate your problems by using BioSep for your GFC/ SEC separations.

2 BioSep - SEC-S HPLC columns for gel filtration / size exclusion chromatography Silica-based GFC / SEC columns for the separation of protein & peptide mixtures For the analysis of antibodies, protein complexes, protein aggregation, and for desalting Maintain biological activity of proteins phase options to successfully separate samples of varying molecular weights Excellent column-to-column reproducibility Economical pricing Easy Column Selection with BioSep Selecting the appropriate column for gel filtration/ size exclusion applications is not always straightforward. Sample details, such as molecular weight and native vs. denatured state, dictate the suitable phase to use. The chart below clearly illustrates which of the BioSep phase options to use based on the characteristics of your sample mixture. Based on Size Based on a Guaranteed Molecular Weight of your Sample Mixture Alternative to the Column You Currently Use Molecular Weight Separation Range for Proteins BioSep-SEC-S Column Cross Reference Chart BioSep SEC-S000 Denatured Under GnHCl S-000 S-000 S-4000 BioSep SEC-S000 Bio-Rad Bio-Sil SEC 50 Agilent Technologies Zorbax GF-450 other manufacturers GFC 000 phases Denatured Under SDS Native S-000 S-000 S-000 S-4000 S-000 S-4000 Bio-Rad Bio-Sil SEC 5 Agilent Technologies Zorbax GF-50 other manufacturers GFC 000 phases Refer to page 5 Refer to page 6 BioSep SEC-S4000 Bio-Rad Bio-Sil SEC 400 Agilent Technologies Molecular Weight (Daltons) other manufacturers GFC 4000 phases Refer to page 6 0 Phenomenex Tel: Fax: www.

3 Increase Efficiency with BioSep How well one protein of interest is separated from another is related to the efficiency of a column; the higher the efficiency the greater the baseline separation between the analytes. As shown in the chart below, BioSep has a very high efficiency, thus making it an excellent column for protein separations via gel filtration/size exclusion. BioSep-SEC-S000 BioSep-SEC-S000 BioSep-SEC-S4000 Resin Type Silica Silica Silica Particle Size (µm) Pore Size (Å) Efficiency (00 x 7.8 mm),000 9,500 8,000 (minimum number theoretical plates) Max Temperature ( C) 50* 50* 50* ph stability * for 6 stainless steel I have been using the BioSep 000 size exclusion columns for the past five years to purify radioiodinated antibodies. These columns have provided us with consistent, reproducible results for a very reasonable and competitive price. In addition, the customer service and technical support provided by Phenomenex is unlike that of any other vendor that I deal with and is truly commendable and a selling point in itself. I would highly recommend this column. Pam Zaller-Farrington - SolidPhase Inc. Proteins on BioSep-SEC-S000 mau min Column: BioSep-SEC-S000 Part No.: 00H-46-K0 Mobile Phase: 00 mm Potassium phosphate buffer, ph 6.8 Flow Rate: ml/min Detection: 80 nm Temperature: C Sample:. Thyroglobulin 4. Myoglobin. Gammaglobulin 5. Uridine. Ovalbumin App ID 5465 If BioSep does not provide you with equal or better separations than any other GFC column of similar porosity, type and comparable dimensions, send in your comparative data within 45 days and keep the BioSep for FREE. Phenomenex Tel: Fax: www. 0

4 Simplify Research with BioSep Characterizing and purifying a protein of interest shouldn t be a complicated, time consuming task. However, when results aren t reproducible, sample loading is limited, and yields are low, that is exactly what it becomes. BioSep can simplify work and reduce time in the lab with its benefits and advantages outlined below. Reproducibility Guaranteed Batch-to-Batch & Column-to-Column Reproducibility of BioSep Each batch of silica-based material is carefully monitored to ensure that particles have the proper size, shape, and pore characteristics batch-to-batch Every column is performance tested to ensure the same high quality separation column-to-column mau 400 & Denote Different Batches a & b Denote Different Columns Batch a Batch a Batch b Batch b I have used the BioSep-SEC-S000 column and have found that it gives good, reproducible profiles of carbohydrate polymers. We use the column as part of a GLP release testing method and require its reproducibility to validate our methods. Anthony Piotrkowski, Gilatech Inc min Column: BioSep-SEC-S000 Part No.: 00H-45-K0 Mobile Phase: 50 mm NaH PO 4 Flow Rate: ml/min Detection: 0 nm Temperature: 0 C Sample:. Amyloglucosidase. Lactalbumin. Ovalbumin 4. Aprotinin App ID 5448, 5449, 5450 & 545 Large Loading Capacity Ensure resolution of critical components regardless of sample loading Excellent choice for prep or process scale separations Within protein folding and activity constraints, column capacity can be further increased with the use of small percentages of organic solvent or with an increase in column temperature Rs- is the resolution between IgM and IgA. Rs- is the resolution between IgA and IgG. Rs- is the resolution between IgG and the void volume. As seen in the figure to the right, the resolution between the peaks of interest remain around.5, the ideal resolution value, from.0 mg up to 6.0 mg. Resolution Between Peaks Loading Capacity of BioSep-SEC-S000 Rs- Rs- Rs Injection, mg Immunoglobulin/00 µl Column: BioSep-SEC-S000 Part No.: 00H-46-K0 Mobile Phase: Potassium phosphate buffer, 0 mm, ph 7.0 Flow Rate: ml/min Detection: 80 nm Sample: Immunoglobulin (IgM, IgA and IgG) dissolved in the mobile phase. Injected to 6 mg in 00 µl App ID Phenomenex Tel: Fax: www.

5 Simplify Research with BioSep Effect of ph on the Distribution Coefficient K D of Lysozyme High Sample Yields and Recovery of Biological Activity K D BioSep -SEC-S4000 BioSep -SEC-S000 BioSep -SEC-S000 Hydrophilically bonded silica gel has low adsorption of proteins resulting in higher yields Relatively little surface activity due to free silanols limits interaction of protein with base silica ph Column: BioSep-SEC-S000, S000 and S4000 in series (x ) Part No.: 00H-45-K0, 00H-46-K0, and 00H-47-K0 Mobile Phase: 00 mm PO 4 buffer, ph Flow Rate: ml/min Detection: 80 nm Sample: Lysozyme 0 mg/ml 0 µl injected App ID 58 The K D values were calculated using the formula: K D = (V e-v o)/(v T-V o) Where V e is the elution volume of the sample, V T and V o are the total liquid volume and void volumes of the columns, respectively. Recovery of Trypsin from BioSep-SEC-S Column ph Protein* % Specific Activity* % BioSep-SEC-S BioSep-SEC-S BioSep-SEC-S *Range for 5 replicate injections The recovery of Trypsin from BioSep-SEC-S is indicative of the protein yield and activity expected from any analytical or preparative BioSep column. 4 5 Lysozyme is a somewhat hydrophobic and very basic protein (pk a.0) which can be used as a probe to test the surface activity of SEC columns. This figure shows only nominal changes in the value of K D for this enzyme when ph was changed. Excellent Column Stability Long column lifetimes due to excellent chemical stability of support materials Adsorbed materials are easily removed by washing with phosphate buffer at ph.0 Strongly retained proteins may be removed by washing with acetonitrile or methanol without compromising performance Column Regeneration Advantage Easily and completely regenerate by flushing with water overnight Restore to non-denaturing conditions quickly and easily I have been using the BioSep-SEC-000 column with the BioSep guard column to estimate the percentage of monomeric form of BSA and IgG for more than three years. Over the last months I critically evaluated the performance of the column using Life Technologies BSA, Sigma amylase. The variation in the retention times of both proteins between runs over the period examined was only 5 %. The mean pressure varied within 0 % between runs. I have enough confidence in the BioSep column that I continue to use this column for the QC tests in our laboratory. I fully recommend the BioSep-SEC-000. Minh Lengoc, Life Technologies Ltd., New Zealand Phenomenex Tel: Fax: www. 04

6 BioSep - SEC-S Column Options The BioSep product line offers different phase options to fit the appropriate exclusion range of your sample mixture. All three phases have a narrow pore size distribution, which produces exceptional separations required for high performance gel filtration/size exclusion. Depending on your analysis needs, BioSep 000, 000, and 4000 are all available in PEEK hardware and in narrow bore, analytical, and preparative dimensions. Broad Molecular Weight Range Separation on BioSep-SEC-S000 Separating a protein mixture of molecular weights from 87 to 00,000 daltons illustrates the wide range that can be easily separated on BioSep-SEC-S000. An excellent linear correlation of is obtained for the log MW vs K D plot. Interpolation of unknown protein is easier and more accurate when the separation is efficient and linear. Exclusion Range in Daltons: Native:,000-00, % SDS: 00-75, Protein and Peptide Size-Exclusion Chromatography on BioSep-SEC-S Log MW BioSep-SEC-S K D I have used BioSep-SEC-000 from Phenomenex and have found that it provides a robust and easy to use application for the comparison of enzymatically treated protein hydrolysates. Tim Emerson, Genencor International BioSep-SEC-S Log MW R = K D min Column: BioSep-SEC-S000 Part No.: 00H-45-K0 Mobile Phase: 50 mm PO 4 buffer, ph 6.8 Flow Rate: ml/min Detection: 5 nm Sample: Proteins + synthetic peptides, 0 µl. lga (00,000). lgg (50,000). Ovalbumin (44,000) 4. Trypsin Inhibitor (0,000) 5. Peptide +5 (,897) 6. Peptide +4 (,9) 7. Peptide + (,6) 8. Peptide + (,595) 9. Peptide + (87) App ID 58 Log MW BioSep-SEC-S4000 R = K D 05 Phenomenex Tel: Fax: www.

7 BioSep - SEC-S Column Options Proteins on BioSep- SEC-S000 Large Proteins on BioSep- SEC- S4000 Mixtures of proteins were subjected to size-exclusion chromatography on BioSep-SEC-S000. Two mg each of the proteins were dissolved in 0.5 ml of the mobile phase buffer, 50 mm NaH PO 4, ph 6.8. Then 0 µl was injected onto the column. The plot of log MW vs. K D was obtained from runs of three different protein mixtures (r = 0.977). BioSep-SEC-S4000 is capable of resolving proteins of high molecular weight ranging in size from 66,000 to 670,000 daltons. The plot of log MW vs. K D shows an excellent linear relationship with a correlation coefficient of Exclusion Range in Daltons: Native: 5, , % SDS: 5,000-00,000 Exclusion Range in Daltons: Native: 5,000 -,000, % SDS: 5, ,000 SEC of Proteins on BioSep-SEC-S000 Protein Gel Filtration on BioSep-SEC-S mau min Column: BioSep-SEC-S000 Dimensions: 600 x 7.8 mm Part No.: 00K-46-K0 Mobile Phase: 50 mm NaH PO 4 buffer, ph 6.8 Flow Rate: 0.5 ml/min Detection: 80 nm Sample: Protein mixture, 0 µl injected. Thyroglobulin, 670 K. IgA, 00 K. IgG, 50 K 4. LDH, 4 K 5. Phosphorylase b., 97 K 6. BSA, 68 K 7. Ovalbumin, 44 K 8. Horse radish peroxidase, 40 K 9. Carbonic anhydrase, 0 K 0. Trypsin inhibitor, 0. K. Myoglobin, 7 K. Lysozyme, 4.4 K. Insulin, 5.7 K 4. Cyanocobalamin,.5 K App ID min Column: BioSep SEC-S4000 Part No: 00H-47-K0 Mobile Phase: 0 mm Sodium Phosphate, ph 7. Flow Rate: 0.5 ml/min Temperature: 0 C Detection: 80 nm Injection Volume: 0 µl (80 µg total injected) Sample: Protein Mixture. Thyroglobulin (670 kda). Apoferritin (44 kda). β-amylase (00 kda) 4. Bovine Serum Albumin (66 kda) App ID 476 Phenomenex Tel: Fax: www. 06

8 BioSep Applications Natural Peptides Proteins Under Denaturating Conditions min Column: BioSep-SEC-S000 Part No.: 00H-45-K0 Mobile Phase: 50 mm PO 4 buffer, ph 6.8 Flow Rate: ml/min Detection: 5 nm Sample: Natural peptides, 0 µl injected. Aprotinin (6,5). Angiotensin (,96). Enkephalin (555) App ID min Column: BioSep-SEC-S000 Part No.: 00H-46-K0 Mobile Phase: 0 mm PO 4 buffer, ph 6.5 with 6 M GnHCI Flow Rate: ml/min Detection: 80 nm Sample: Protein mixture, 0 µl. Phosphorylase b (97,000). BSA (68,000). Ovalbumin (44,000) 4. Carbonic Anhydrase (0,000) 5. Trypsin Inhibitor (0,00) 6. Lysozyme (4,400) 7. -mercaptoethanol App ID 588 Poly (Gamma Glutamic Acid) Conjugated with Porcine Somatotropic Hormone Chromatogram courtesy of Dr. Joel Swadesh, Alpha Beta Technologies, Boston, MA Polyaspartic Standards Chromatogram courtesy of Mr. Lou DeLuca and Dr. A. P. Wheeler, Department of Biological Sciences, Clemson University, SC min Column: BioSep-SEC-S000 Part No.: 00H-46-K0 Mobile Phase: 0 mm PO 4 buffer, ph 7.0 Flow Rate:.0 ml/min Detection: 0 nm Temperature: Ambient Sample:. Conjugate. Porcine Somatotropic Hormone. Activated Poly (Gamma Glutamic Acid) App ID min Column: BioSep-SEC-S000 Part No.: 00H-45-K0 Mobile Phase: 0. M Acetate/0.05 M Tris Acetate ph 7.5 Flow Rate: ml/min Detection: 54 nm Temperature: Ambient Sample:. Asp-45 (0.4). Asp-5 (.5). Asp-5 (0.9) App ID 589 Human Serum Albumin Protein Standards * Bio-Rad protein standard was reconstituted according to manufacturer s instructions min Column: BioSep-SEC-S000 Part No.: 00H-46-K0 Mobile Phase: 0 mm NaH PO 4, ph 7. Flow Rate: ml/min Detection: 5 nm Sample: Human serum albumin 0.5 mg/ml, 0 µl injected. Aggregate. Albumin App ID min Column: BioSep-SEC-S000 Part No.: 00H-46-K0 Mobile Phase: 50 mm PO 4 buffer, ph 6.8 Flow Rate: ml/min Detection: 80 nm Sample: Bio-Rad protein standard, *0 µl injected. High MW Aggregates. Thyroglobulin. IgA 4. IgG 5. Ovalbumin 6. Myoglobin 7. Cyanocobalamin App ID Phenomenex Tel: Fax: www.

9 BioSep Applications Immunoglobulins on BioSep-SEC-S000 Desalting of Lactalbumin in M Salt 00 mau min 00 mau min Column: BioSep-SEC-S000 Part No.: 00H-46-K0 Mobile Phase: 50 mm NaH PO 4, ph 6.8 Flow Rate: ml/min Detection: 80 nm Sample: Bovine immunoglobulins, 8.0 mg/ml, 40 µl injected. IgM. IgA. IgG App ID min Column: BioSep-SEC-S000 Part No.: 00H-45-K0 Mobile Phase: 50 mm NaH PO 4 Flow Rate: ml/min Detection: 54 nm Temperature: 5 C Sample:. Lactalbumin aggregate. Lactalbumin. Salt contaminant 4. Salt contaminant App ID 4995, 4996 My peak shape was greatly improved. Prior to using BioSep-SEC-S000 my peak width was greater than minutes for chondroitin sulfate. With the 000 my peak width is about 0 Craig Marshall, IAMS Company seconds. BioSep-SEC-S Technical Data and Specifications BioSep-SEC-S000 BioSep-SEC-S000 BioSep-SEC-S4000 Material Hydrophilic bonded silica Hydrophilic bonded silica Hydrophilic bonded silica Particle Size (µm) Pore Size (Å) Exclusion Range in Daltons for Proteins: Native,000-00,000 5, ,000 5,000-,000, % SDS 00-75,000 5,000-00,000 5, ,000 6 M GnHCl ,000,000-50,000 5, ,000 ph Range.5 to to to 7.5 Typical Backpressure 450 psi 50 psi 00 psi (for 00 x 7.8 mm column at ml/min) Maximum 000 psi 000 psi 000 psi Backpressure Efficiency Minimum plates/meter (using uridine with H O as mobile phase) (00 x 7.8 mm) 70,000 65,000 60,000 Maximum Flow Rate This is a function of pressure. Columns can withstand up to,000 psi, but avoid sudden pressure changes. Column Hardware Standard: 6 stainless steel column with µm steel frits. Biocompatible columns (PEEK) with µm PEEK frits also available Maximum Temp. 50 C for 6 stainless steel; ambient temperature for PEEK Maximum Salt Conc. 0.5 M Denaturants 0.5 % SDS, 6 M Guanidine HCl, or 8 M urea Regeneration After exposure to denaturants, wash immediately with water overnight. Max. Organic Modifier Up to 00 % CH CN. Start with 00 % H O, linear gradient to 00 % CH CN over 50 min. Up to 90 % CH OH, 0 % DMSO or 500 mm β-mercaptoethanol. Cleaning Procedure General protein removal: wash with 0 ml of 0. M NaH PO 4, ph.0. Hydrophobic protein removal: use acetonitrile gradient. Strongly adsorbed proteins: wash with 0 ml of 0.5% SDS or 6 M Gn thiocyanate or 0 % DMSO. Storage Overnight storage: run mobile phase at 0. ml/minute. Prolonged storage: use 0.05 % NaN in H O or 0 % methanol in H O. General Use of a guard column is recommended to prolong column lifetime. Phenomenex Tel: Fax: www. 08

10 BioSep Ordering Information Ordering Information Other column dimensions available upon request. Stainless Steel Columns (mm): Narrow Bore Analytical Preparative 00 x x x x. 600 x. Phases 000 I $,0 00H-45-E0 $,50 00H-45-K0 I $,880 00K-45-K0 $,50 00H-45-P0 I $4,700 00K-45-P H-46-E0 00H-46-K0 00K-46-K0 00H-46-P0 00K-46-P H-47-E0 00H-47-K0 00K-47-K0 00H-47-P0 00K-47-P0 SecurityGuard Cartridges require universal holder Order No.: KJO-48 Stainless Steel Guards (mm): SecurityGuard Narrow Bore Express Analytical Preparative Cartridges 0 x x x x. 4 x.0mm Phases $0 $5 $75 $75 $99/0 pk 000 I 0A-45-E0 0Q-45-K0 0C-45-K0 0R-45-P0 AJ A-46-E0 0Q-46-K0 0C-46-K0 0R-46-P0 AJ A-47-E0 0Q-47-K0 0C-47-K0 0R-47-P0 AJ for ID:.-8.0 mm PEEK (Biocompatible) Columns (mm): PEEK (Biocompatible) Guards (mm): Narrow Bore Analytical Narrow Bore Analytical 00 x x x x 7.5 Phases $,475 $,550 Phases $90 $ I 00H-45-E0-BV 00H-45-J0-BV 000 0A-45-E0-BV 0C-45-J0-BV H-46-E0-BV 00H-46-J0-BV 000 0A-46-E0-BV 0C-46-J0-BV H-47-E0-BV 00H-47-J0-BV A-47-E0-BV 0C-47-J0-BV Aqueous SEC Column Check Standard Part No.: ALO-04 Price: (For BioSep SEC-S and other protein SEC columns) $ 9 Unit quantity: Dry; Reconstituted to ml Contains: Bovine thyroglobulin; Human gamma globulin; Ovalbumin; Myoglobin; Uridine (reconstitute with ml of 00 mm Phosphate buffer ph=6.8) Diluent: 00 mm Sodium phosphate buffer, ph 6.8 Storage: Add 0. % NaN to the solution and refrigerate Test Conditions Mobile phase: 00 mm Sodium phosphate buffer, ph 6.8 Flow rate:.0 ml/min for a 00 x 7.8 mm column Injection volume: 0 µl Detection: 80 nm App ID 475 FREE GUIDE to Protein and Peptide Analysis by HPLC Theory and application of protein and peptide separation techniques Practical guidelines for selecting columns, developing and optimizing separations Useful tips and traps on column care, cleaning and storage Review of Micro LC in Biochromatography AA0-908 BioSep, Phenex, and SecurityGuard are trademarks of Phenomenex, Inc. Bio-Sil is a registered trademark of Bio-Rad. Zorbax GF is a registered trademark of Aglient Technologies. Puradisc is a trademark of Whatman. Phenomenex is in no way affiliated with the above companies. Columns used for comparison were purchased directly from the original manufacturer. Comparisons shown may not be representative of every application. Information, descriptions, and specifications in this publication are subject to change without notice. 007 Phenomenex, Inc. All rights reserved. Call your Phenomenex representative for your FREE copy! If BioSep does not provide you with equal or better separations than any other GFC column of similar porosity, type and comparable dimensions, send in your comparative data within 45 days and keep the BioSep for FREE. Subject to Phenomenex Standard Terms & Conditions, which may be viewed at 09 Phenomenex Tel: Fax: www.

11 Phenex Syringe Filters & Membranes For Sample and Solvent Filtration Prior to Chromatography! Phenex Syringe Filters Rapid filtration of HPLC and GC samples prior to analysis Particulate, PVC, and extractable-free filters Consistent, reliable performance Phenex Features Low absorption Particulate-free Maximum chemical compatibility Minimum extractables Excellent flow rate High total throughput Ordering Information Low hold-up volume Certified quality 00 % integrity tested Easy pore identification PVC-free Bi-directional use Tip: Try a Sample Pack! The best way to determine if a specific Phenex membrane is suitable for your application. Request yours today by phone or visit Phenex Syringe Filters 4 mm Diameter 5 mm Diameter 5 mm Diameter for < ml sample volumes for - 0 ml sample volumes for 0-00 ml sample volumes Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price 0.45 µm Phenex-RC AF /Pk $5 AF /Pk $55 AF /Pk $95 (Regenerated Cellulose) AF /Pk 540 AF /Pk 60 AF /Pk 780 Phenex-PES AF /Pk 80 AF /Pk 5 (Polyethersulfone) AF /Pk 860 Phenex-PTFE 6 AF /Pk 45 AF /Pk 80 AF /Pk 5 (Polytetrafluoroethylene) AF /Pk 580 AF /Pk 70 AF /Pk 860 Phenex-NY AF-07-00/Pk 40 AF /Pk 60 AF /Pk 05 (Nylon) AF /Pk 560 AF /Pk 640 AF /Pk 80 Phenex-GF/CA,,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8B09-00/Pk 45 (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8B /Pk µm Phenex-RC AF /Pk $5 AF /Pk $55 AF /Pk $95 (Regenerated Cellulose) AF /Pk 540 AF /Pk 60 AF /Pk 780 Phenex-PES AF /Pk 5 (Polyethersulfone) AF /Pk 860 Phenex-PTFE 6 AF /Pk 45 AF /Pk 80 AF /Pk 5 (Polytetrafluoroethylene) AF /Pk 580 AF /Pk 70 AF /Pk 860 Phenex-NY AF-07-00/Pk 40 AF /Pk 60 AF /Pk 05 (Nylon) AF /Pk 560 AF /Pk 640 AF /Pk 80 Phenex-GF/CA,,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8A09-00/Pk 45 (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8A /Pk µm Phenex-GF Prefiltration of heavily contaminated or highly viscous samples. When used in-line preceding AF /Pk $50 (Glass Fiber) a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. AF /Pk mm diameter.. Glass fiber filters are 6 mm diameter and made of borosilicate. They will remove 90 % of all particles >. µm.. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cryolite. 4. Cellulose acetate is surfactant-free mm diameter. 6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA. 7. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance. 8. Larger quantity purchases at significant savings are available. If Phenex Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, send in your comparative data within 45 days and keep the Phenex products for FREE! Phenomenex Tel: Fax: www. 0

12 4 Madrid Avenue Torrance, CA U.S.A. PRSRT STD U.S. POSTAGE PAID PERMIT #85 TORRANCE, CA Tired of paying high prices? Irritated with slow delivery times? Frustrated by irreproducible results? Eliminate your problems by using BioSep for your GFC/ SEC separations. www. Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department by telephone, fax or mail: tel.: fax: USA 4 Madrid Ave. Torrance, CA USA (0) (0) info@ Australia PO Box 4084 Lane Cove, NSW 066 Australia auinfo@ Austria Zeppelinstr Aschaffenburg Germany anfrage@ Canada 4 Madrid Ave. Torrance, CA USA (800) (0) info@ Denmark Gydevang Allerød Denmark dkinfo@ France Parc des Grillons, Bat. 60 route de Sartrouville 78 Le Pecq Cedex France franceinfo@ 557_us mail: tel.: fax: Germany Zeppelinstr Aschaffenburg Germany anfrage@ Ireland Queens Avenue, Hurdsfield Ind. Est., Macclesfield, Cheshire SK0 BN, UK eireinfo@ Italy Via Serenari, 5/D 400 Castel Maggiore (BO) Italy italiainfo@ New Zealand P O Box -60 Milford 074 North Shore City New Zealand nzinfo@ Puerto Rico 7 Sierra Morena, Suite #04 San Juan, Puerto Rico 0096 (800) 54-HPLC (0) info@ United Kingdom Queens Avenue, Hurdsfield Ind. Est., Macclesfield, Cheshire SK0 BN, UK ukinfo@