HiPer Real-Time PCR Teaching Kit

Size: px
Start display at page:

Download "HiPer Real-Time PCR Teaching Kit"

Transcription

1 HiPer Real-Time PCR Teaching Kit Product Code: HTBM032 Number of experiments that can be performed: 10 Duration of Experiment Protocol: 1.5 hours Storage Instructions: The kit is stable for 12 months from the date of receipt Store Hi-SYBr Master Mix, Primer Mix, Molecular Biology Grade Water and Template DNA at -20 o C Other kit contents can be stored at room temperature (15-25 o C) 1

2 Index Sr. No. Contents Page No. 1 Aim 3 2 Introduction 3 3 Principle 3 4 Kit Contents 5 5 Materials Required But Not Provided 5 6 Storage 5 7 Important Instructions 5 8 Procedure 6 9 Observation and Result 7 10 Interpretation 7 11 Troubleshooting Guide 7 2

3 Aim: To learn the process of Real-time PCR. Introduction: Real-time polymerase chain reaction is a molecular biology based laboratory technique for amplification and simultaneous quantification of a targeted DNA. This specialized reaction follows the principles of regular PCR in real time instead of detecting the end product at the end. For this reason it is called quantitative PCR which identifies the products using either a fluorescent dye or a probe. Principle: Polymerase Chain Reaction (PCR) is a very sensitive and specific method to amplify a precise fragment of DNA from a complex mixture of starting material called as template DNA. The three steps of a successful PCR reaction include Denaturation, Annealing and Extension which consists of a series of temperature changes that are repeated times and performed in a specialized instrument called thermal cycler. The Real-time PCR or Quantitative PCR is a modified approach compared to standard PCR, where the amplified DNA is detected as the reaction progresses instead of detection at the end. Quantitative PCR is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of a specified wavelength and detect the fluorescence emitted by the excited fluorophore. There are two methods for the detection of amplified products in Real-time PCR: 1. Using non-specific fluorescent dyes SYBR Green is a double-stranded (ds) DNA binding fluorescent dye which is excited using blue light (λ max = 488 nm) and it emits green light (λ max = 522 nm). It intercalates to ds DNA during PCR which results in fluorescence. Therefore, an increase in DNA product during PCR leads to an increase in fluorescence intensity that can be measured for each cycle and hence, DNA concentrations are quantified. However, SYBR Green binds to all dsdna PCR products which include nonspecific PCR products like primer-dimer. This can potentially interfere with, or prevent, accurate quantification of the intended target sequence. Forward Primer SYBR Green Forward Primer SYBR Green I II Excitation Polymerization DNA Polymerase Emission III IV Fig 1: The sequence of reactions during SYBR Green-based Real-Time PCR At the beginning of PCR, the reaction mixture contains denatured DNA, primers, and SYBR Green dye. The unbound dye molecules give a weak fluorescence that produces a minimal background signal which is subtracted during data analysis. During the elongation step, more and more dye molecules intercalate to the newly synthesized DNA. An increase in fluorescence signal is observed in real-time by a constant 3

4 monitoring of the reaction. When the DNA is denatured during the next heating cycle, the dye molecules are released and the fluorescence signal decreases. 2. Using sequence-specific Hydrolysis (TaqMan) DNA probes In this real-time PCR a sequencespecific oligonucleotide is labeled with a fluorescent reporter and allows detection when it hybridizes to its complementary sequence. Fluorescent reporter probes detect only the DNA containing the probe sequence; therefore, use of the reporter probe significantly increases specificity, and enables quantification even in the presence of non-specific DNA amplification. Fig 2: The sequence of reactions during Hydrolysis (TaqMan) based Real-Time PCR In this real-time PCR the fluorescent reporter is tagged at one end of the probe and a quencher of fluorescence at the opposite end. The fluorescence is prevented when the reporter comes in close proximity to the quencher. During the annealing and extension steps the probe hybridizes to the target, and the dsdna-specific 5' 3' exonuclease activity of Taq cleaves off the reporter. As a result, the reporter is separated from the quencher which generates fluorescence signal that is proportional to the amount of amplified product in the sample. The HiPer Real-time PCR Teaching Kit is designed for detection of specific sequence of a specific gene (450 bp) for E.coli O157 using SYBR Green based quantitative PCR technique. 4

5 Kit Contents: Table 1: Enlists the materials provided in this kit with their quantity and recommended storage. Sr. Quantity Product Code Materials Provided No. 10 expts Storage 1 MBT074* Hi-SYBr Master Mix ml -20 o C 2 TKC394* Primer Mix ml -20 o C 3 ML065 Molecular Biology Grade Water for RT-PCR 1 ml -20 o C 4 TKC395* Template DNA ml -20 o C 5 CG282 Polypropylene Tubes, 0.2 ml (PCR Tubes) 22 No R T * Always give a quick spin before opening the vial as the liquid material may stick to the wall or to the cap of the vial Materials Required But Not Provided: Real-time Thermocycler, Vortex Mixer, Centrifuge, Micropipettes, Tips (preferably barrier), Crushed ice Storage: HiPer Real-time PCR Teaching Kit is stable for 12 months from the date of receipt without showing any reduction in performance. On receipt, store Hi-SYBr Master Mix, Primer Mix, Molecular Biology Grade Water and template DNA at -20 o C. Other reagents can be stored at room temperature (15-25 o C). Important Instructions: Before use all PCR components should be completely thawed on ice. Perform the amplification reactions in a clean area, preferably in a biosafety cabinet. Use of aerosol barrier pipette tips is recommended to reduce contamination risks from extraneous DNA templates. 5

6 Procedure: Flow Chart for setting up PCR Reaction Add 10µl Hi-SYBr master mix in a PCR tube and label it as +ve Add 10µl Hi-SYBr master mix in a PCR tube and label it as -ve Add 2 µl the Primer mix Add 2 µl the Primer mix Add 1µl of template DNA Add 7 µl of Molecular Biology Grade Water Add 8 µl of Molecular Biology Grade to make the final volume to 20 µl Water to make the final volume to 20 µl Centrifuge the tube briefly at 6000 rpm for about 10 seconds Centrifuge the tube briefly at 6000 rpm for about 10 seconds Place the tubes in real-time PCR machine and set the recommended PCR program Place the tubes in real-time PCR machine and set the recommended PCR program Initial denaturation at 95 o C for 10 minutes Denaturation at 95 o C for 45seconds Annealing at 56 o C for 30 seconds 27 cycles Extension at 72 o C for 30 seconds Final Extension at 72 o C for 10 minutes 6

7 Observation and Result: Analyze the data from the amplification plot and observe the Ct values: Sr. No. Sample C t value 1 Negative control N/A 2 1 µl of template DNA (amplicon of E. coli O157:H7) 3 1 µl of template (amplicon of E. coli O157:H7) Fig 3: Data representing real-time amplification of E. coli O157:H7 with C t values (provided in table) Interpretation: After performing the real-time PCR amplification of E. coli O157:H7 DNA one can analyze the data from the amplification plot and obtain the C t value. Troubleshooting Guide: Sr.No. Problem Solution Cause 1. No amplification Degraded samples Check the integrity of DNA using agarose gel electrophoresis. Error in protocol setup Verify that the correct reagent volumes, dilutions and storage conditions have been used. 2. Variability between Error in reaction set-up Prepare large volume master mix, vortex thoroughly and aliquot into reaction tubes. replicates Air bubbles in reaction mix Briefly centrifuge reaction samples/plate prior to running on a real-time PCR instrument. Pipetting error C t values of replicates can show increased variation due to poor laboratory technique or imprecise pipettes. 3. Amplification in Reagents contaminated Repeat the analysis. negative control Technical Assistance: At HiMedia we pride ourselves on the quality and availability of our technical support. For any kind of Technical assistance mail at PIHTBM032 _O/0814 HTBM

HiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit

HiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit HiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit Product Code: HTBM031 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 3.5 hours Agarose Gel Electrophoresis:

More information

HiPer RT-PCR Teaching Kit

HiPer RT-PCR Teaching Kit HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The

More information

Technical Review. Real time PCR

Technical Review. Real time PCR Technical Review Real time PCR Normal PCR: Analyze with agarose gel Normal PCR vs Real time PCR Real-time PCR, also known as quantitative PCR (qpcr) or kinetic PCR Key feature: Used to amplify and simultaneously

More information

HELINI Hepatitis B virus [HBV] Real-time PCR Kit (Genotype A to H)

HELINI Hepatitis B virus [HBV] Real-time PCR Kit (Genotype A to H) HELINI Hepatitis B virus [HBV] Real-time PCR Kit (Genotype A to H) Quantitative In vitro diagnostics Instruction manual Cat. No: 8001-25/50/100 tests Compatible with: Agilent, Bio-Rad, Applied Bio systems

More information

HiPer Gel Extraction Teaching Kit (Column Based)

HiPer Gel Extraction Teaching Kit (Column Based) HiPer Gel Extraction Teaching Kit (Column Based) Product Code: HTBM010 Number of experiments that can be performed: 10 Duration of Experiment Agarose Gel Electrophoresis: 1 hour Protocol: 1 hour Agarose

More information

foodproof SL GMO Maize Multiplex Detection Kit

foodproof SL GMO Maize Multiplex Detection Kit For food testing purposes. FOR IN VITRO USE ONLY. foodproof SL GMO Maize Multiplex Detection Kit (MON88017, NK603, MIR162) - 5 Nuclease - Version 1, November 2014 PCR kit for the qualitative detection

More information

foodproof SL GMO Maize Multiplex Detection Kit

foodproof SL GMO Maize Multiplex Detection Kit For food testing purposes. FOR IN VITRO USE ONLY. foodproof SL GMO Maize Multiplex Detection Kit (MON89034, CBH351, Bt176) - 5 Nuclease - Version 1, November 2014 PCR kit for the qualitative detection

More information

SunScript TM One Step RT-qPCR Kit

SunScript TM One Step RT-qPCR Kit INDEX Ordering Information...3 Kit Contents...3 Shipping and Storage...3 Handling...3 Quality Control...3 Reagents and Equipment to be Supplied by the User...3 Description...4 Protocol...4 Troubleshooting

More information

Cat. # RR391A. For Research Use. Probe qpcr Mix. Product Manual. v201610da

Cat. # RR391A. For Research Use. Probe qpcr Mix. Product Manual. v201610da Cat. # RR391A For Research Use Probe qpcr Mix Product Manual Table of Contents I. Introduction... 3 II. Principle... 3 III. Components... 5 IV. Materials Required but not Provided... 5 V. Storage... 5

More information

Real Time PCR. Advanced Biotechnology Lab I Florida Atlantic University April 2, 2008

Real Time PCR. Advanced Biotechnology Lab I Florida Atlantic University April 2, 2008 Real Time PCR Advanced Biotechnology Lab I Florida Atlantic University April 2, 2008 Introduction We wish to compare the expression levels of our gene under study (Drosophila MsrA) for two different treatment

More information

User Manual. NGS Library qpcr Quantification Kit (Illumina compatible)

User Manual. NGS Library qpcr Quantification Kit (Illumina compatible) NGS Library qpcr Quantification Kit (Illumina compatible) User Manual 384 Oyster Point Blvd, Suite 15 South San Francisco, CA 94080 Phone: 1 (888) MCLAB-88 Fax: 1 (650) 872-0253 www.mclab.com Contents

More information

Premix Ex Taq (Probe qpcr)

Premix Ex Taq (Probe qpcr) For Research Use Premix Ex Taq (Probe qpcr) Product Manual Table of Contents I. Description... 3 II. Principle... 4 III. Components... 5 IV. Materials Required but not Provided... 5 V. Storage... 5 VI.

More information

DIRECTION FOR USE. Art. No / /25 reactions in vitro Diagnosticum for birds

DIRECTION FOR USE. Art. No / /25 reactions in vitro Diagnosticum for birds DIRECTION FOR USE Art. No. 31066 / 31067 100 /25 reactions in vitro Diagnosticum for birds Kylt Turkey Hemorrhagic Enteritis Virus PCR Detection Kit is for detection of the etiological agent of Infectious

More information

SunScript One Step RT-PCR Kit

SunScript One Step RT-PCR Kit SunScript ONE STEP R T-PCR KIT HANDBOOK SunScript One Step RT-PCR Kit INDEX Legal... 4 Intended use... 4 Kit contents... 5 Shipping and storage... 5 Handling... 6 Quality control... 6 Reagents and equipment...

More information

CBFB-MYH11 REAL TIME QUANTITATIVE PCR DETECTION KIT ONKOTEST RQ

CBFB-MYH11 REAL TIME QUANTITATIVE PCR DETECTION KIT ONKOTEST RQ CBFB-MYH11 REAL TIME QUANTITATIVE PCR DETECTION KIT ONKOTEST RQ3021-20 Product Information Inv(16)(p13q22) or the variant t(16;16)(p13;q22) are frequent recurring chromosomal rearrangements reported to

More information

Quantitative Real time PCR. Only for teaching purposes - not for reproduction or sale

Quantitative Real time PCR. Only for teaching purposes - not for reproduction or sale Quantitative Real time PCR PCR reaction conventional versus real time PCR real time PCR principles threshold cycle C T efficiency relative quantification reference genes primers detection chemistry GLP

More information

foodproof SL Staphylococcus aureus Detection Kit

foodproof SL Staphylococcus aureus Detection Kit For food testing purposes. FOR IN VITRO USE ONLY. foodproof SL Staphylococcus aureus Detection Kit - 5 Nuclease - Version 1, October 2014 PCR kit for the qualitative detection of Staphylococcus aureus

More information

For in vitro Veterinary Diagnostics only. PCR Detection Kit for Fowl Adenovirus.

For in vitro Veterinary Diagnostics only. PCR Detection Kit for Fowl Adenovirus. For in vitro Veterinary Diagnostics only. PCR Detection Kit for Fowl Adenovirus www.kylt.eu DIRECTION FOR USE Art. No. 31144 / 31145 Kylt Fowl Adenovirus PCR Detection Kit for Fowl Adenovirus 100 /25 reactions

More information

SYBR Premix Ex Taq II (Tli RNaseH Plus), Bulk

SYBR Premix Ex Taq II (Tli RNaseH Plus), Bulk Cat. # RR820L For Research Use SYBR Premix Ex Taq II (Tli RNaseH Plus), Bulk Product Manual The long-term storage temperature of this product has been changed to -20 since Lot. AK9104. See section IV.

More information

BRCA MAQ USER GUIDE Version 1.0

BRCA MAQ USER GUIDE Version 1.0 BRCA MAQ USER GUIDE Version 1.0 For Copy Number Analysis Assays IFU604 v170797 www.multiplicom.com 2012 Multiplicom NV, all rights reserved Table of Contents Introduction to Multiplex Amplicon Quantification...2

More information

CBFB-MYH11 REAL TIME PCR DETECTION KIT

CBFB-MYH11 REAL TIME PCR DETECTION KIT CBFB-MYH11 REAL TIME PCR DETECTION KIT ONKOTEST R3020-20 Keep the kit at -15 C to -25 C Rev. 1.4 Product Information Inv(16)(p13q22) or the variant t(16;16)(p13;q22) are frequent recurring chromosomal

More information

2x PCR LongNova-RED PCR Master Mix

2x PCR LongNova-RED PCR Master Mix 2x PCR LongNova-RED Components RP85L 100 reactions (50 μl) RP85L-10 1000 reactions (50 μl) 2x PCR LongNova-RED 2 x 1.25 ml 20 x 1.25 ml PCR grade water 2 x 1.5 ml 20 x 1.5 ml Storage & Shiing Storage conditions

More information

Brilliant II Fast SYBR Green QPCR Master Mix

Brilliant II Fast SYBR Green QPCR Master Mix Brilliant II Fast SYBR Green QPCR Master Mix INSTRUCTION MANUAL Catalog #600843 (single kit) #600844 (10-pack kit) Revision B.01 For In Vitro Use Only 600843-12 LIMITED PRODUCT WARRANTY This warranty limits

More information

Only for teaching purposes - not for reproduction or sale

Only for teaching purposes - not for reproduction or sale PCR reaction conventional versus real time PCR real time PCR principles threshold cycle C T efficiency relative quantification reference genes primers detection chemistry GLP in real time PCR Relative

More information

For in vitro Veterinary Diagnostics only. PCR Detection Kit for Salmonella Gallinarum, Pullorum (separate detection) & DIVA 9R.

For in vitro Veterinary Diagnostics only. PCR Detection Kit for Salmonella Gallinarum, Pullorum (separate detection) & DIVA 9R. For in vitro Veterinary Diagnostics only. PCR Detection Kit for Salmonella Gallinarum, Pullorum (separate detection) & DIVA 9R www.kylt.eu DIRECTION FOR USE Art. No. 31420 / 31421 Kylt SGP & 9R DIVA PCR

More information

Brilliant II SYBR Green QPCR Master Mix

Brilliant II SYBR Green QPCR Master Mix Brilliant II SYBR Green QPCR Master Mix INSTRUCTION MANUAL Catalog #600828 (single kit) #600831 (10-pack kit) Revision B.01 For In Vitro Use Only 600828-12 LIMITED PRODUCT WARRANTY This warranty limits

More information

Q-PCR QUANTITATIVE-PCR 세포생물학및실험 2 박태식교수님

Q-PCR QUANTITATIVE-PCR 세포생물학및실험 2 박태식교수님 Q-PCR QUANTITATIVE-PCR 세포생물학및실험 2 박태식교수님 Title : Study that the Drug A inhibits the accumulation of fat. Schedules 1. Mouse necropsy : take up the blood and major tissues(organs) ex) heart, liver, fat,

More information

A Recommended Procedure for Real-Time Quantitative TaqMan PCR for Roundup Ready Canola RT73 Monsanto Biotechnology Regulatory Sciences

A Recommended Procedure for Real-Time Quantitative TaqMan PCR for Roundup Ready Canola RT73 Monsanto Biotechnology Regulatory Sciences Page 1 of 7 Overview Purpose & Scope This procedure describes an event-specific real-time TaqMan PCR method for determination of the relative content of Roundup Ready canola RT73 (hereafter referred to

More information

One Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time)

One Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time) Cat. # RR086A For Research Use One Step SYBR PrimeScript RT-PCR Kit II Product Manual Table of Contents I. Description...3 II. III. IV. Principle...3 Components...5 Storage...6 V. Features...6 VI. VII.

More information

HELINI White spot Syndrome virus [WSSV] Real-time PCR Kit

HELINI White spot Syndrome virus [WSSV] Real-time PCR Kit HELINI White spot Syndrome virus [WSSV] Real-time PCR Kit Instruction manual Cat. No: 6001-25/50/100 tests Compatible with: Agilent, Bio-Rad, Roche, Applied Bio systems [ABI], Rotor-gene, Cepheid, Bioer,

More information

Real-Time PCR Principles and Applications

Real-Time PCR Principles and Applications Real-Time PCR Principles and Applications Dr Esam Ibraheem Azhar (BSc, MSc, Ph.D Molecular Medical Virology) Asst. Prof. Medical Laboratory Technology Department Objectives Real-Time PCR Principles and

More information

For in vitro Veterinary Diagnostics only. DNA Extraction and PCR Detection Kit for Pasteurella multocida.

For in vitro Veterinary Diagnostics only. DNA Extraction and PCR Detection Kit for Pasteurella multocida. For in vitro Veterinary Diagnostics only. DNA Extraction and PCR Detection Kit for Pasteurella multocida www.kylt.eu DIRECTION FOR USE Art. No. 31058 / 31059 Kylt Pasteurella multocida DNA Extraction and

More information

3color RT HS-PCR Mix SYBR Ready-to-use mix for real-time Hot Start PCR with SYBR Green. Dedicated for white reaction tubes and plates.

3color RT HS-PCR Mix SYBR Ready-to-use mix for real-time Hot Start PCR with SYBR Green. Dedicated for white reaction tubes and plates. 3color RT HS-PCR Mix SYBR Ready-to-use mix for real-time Hot Start PCR with SYBR Green. Dedicated for white reaction tubes and plates. version 0217 250 reactions in 20 μl Cat. # 2000-250S 2500 reactions

More information

For in vitro Veterinary Diagnostics only. Kylt E. coli F18, F41, Stx2e. Real-Time PCR Detection.

For in vitro Veterinary Diagnostics only. Kylt E. coli F18, F41, Stx2e. Real-Time PCR Detection. For in vitro Veterinary Diagnostics only. Kylt E. coli F18, F41, Stx2e Real-Time PCR Detection www.kylt.eu DIRECTION FOR USE Kylt E. coli F18, F41, Stx2e Real-Time PCR Detection A. General Kylt E. coli

More information

SuperTCRExpress TM Human TCR Vβ Repertoire CDR3 Diversity Determination (Spectratyping) and Quantitative Analysis Kit

SuperTCRExpress TM Human TCR Vβ Repertoire CDR3 Diversity Determination (Spectratyping) and Quantitative Analysis Kit SuperTCRExpress TM Human TCR Vβ Repertoire CDR3 Diversity Determination (Spectratyping) and Quantitative Analysis Kit Cat. No. H0521 Size: 2 sets (22 Vβ families/each, with enzymes) H0522 Size: 4 sets

More information

SuperReal PreMix Plus (SYBR Green)

SuperReal PreMix Plus (SYBR Green) SuperReal PreMix Plus (SYBR Green) For fast, quantitative, real-time PCR using SYBR Green www.tiangen.com/en QP120627 SuperReal PreMix Plus (SYBR Green) Cat. no. FP205 Kit Contents Contents 2 SuperReal

More information

Telomerase Activity Quantification qpcr Assay Kit (TAQ) Catalog # reactions

Telomerase Activity Quantification qpcr Assay Kit (TAQ) Catalog # reactions Telomerase Activity Quantification qpcr Assay Kit (TAQ) Catalog #8928 100 reactions Product Description Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes

More information

qpcr Kit, DNA-free Product components 100 rxn 250 rxn Product description

qpcr Kit, DNA-free Product components 100 rxn 250 rxn Product description qpcr Kit, DNA-free For the PCR detection and identification of bacterial and fungal DNA using custom primers Product code A8514 Product components 100 rxn 250 rxn A 2.5x mastermix (3 mm MgCl 2 final concentration)

More information

SYBR Premix Ex Taq II (Tli RNaseH Plus), ROX plus

SYBR Premix Ex Taq II (Tli RNaseH Plus), ROX plus Cat. # RR82LR For Research Use SYBR Premix Ex Taq II (Tli RNaseH Plus), ROX plus Product Manual The long-term storage temperature of this product has been changed to -20 since Lot. A1901A. See section

More information

For in vitro Veterinary Diagnostics only. Real-Time PCR Detection Kit for detection of Histomonas meleagridis.

For in vitro Veterinary Diagnostics only. Real-Time PCR Detection Kit for detection of Histomonas meleagridis. For in vitro Veterinary Diagnostics only. Real-Time PCR Detection Kit for detection of Histomonas meleagridis www.kylt.eu DIRECTION FOR USE Art. No. 31416/31417 Kylt Histomonas meleagridis Real-Time PCR

More information

mirna QPCR Master Mix

mirna QPCR Master Mix mirna QPCR Master Mix Instruction Manual Catalog #600583 Revision B Research Use Only. Not for Use in Diagnostic Procedures. 600583-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement

More information

TB Green Premix Ex Taq II (Tli RNaseH Plus)

TB Green Premix Ex Taq II (Tli RNaseH Plus) Cat. # RR820A For Research Use TB Green Premix Ex Taq II (Tli RNaseH Plus) Product Manual The long-term storage temperature of this product has been changed to -20 since Lot. #AGY1013N. See section IV.

More information

TB Green Premix Ex Taq (Tli RNaseH Plus)

TB Green Premix Ex Taq (Tli RNaseH Plus) Cat. # RR420A For Research Use TB Green Premix Ex Taq (Tli RNaseH Plus) Product Manual The long-term storage temperature of this product has been changed to -20 since Lot. #AK8101. See section V. Storage.

More information

For in vitro Veterinary Diagnostics only. Kylt Brachyspira spp. Real-Time PCR Detection.

For in vitro Veterinary Diagnostics only. Kylt Brachyspira spp. Real-Time PCR Detection. For in vitro Veterinary Diagnostics only. Kylt Brachyspira spp. Real-Time PCR Detection www.kylt.eu DIRECTION FOR USE Kylt Brachyspira spp. Real-Time PCR Detection A. General Kylt Brachyspira spp. products

More information

mirna QPCR Master Mix

mirna QPCR Master Mix mirna QPCR Master Mix Instruction Manual Catalog #600583 Revision D.0 For Research Use Only. Not for use in diagnostic procedures. 600583-12 LIMITED PRODUCT WARRANTY This warranty limits our liability

More information

Brilliant SYBR Green QPCR Master Mix

Brilliant SYBR Green QPCR Master Mix Brilliant SYBR Green QPCR Master Mix INSTRUCTION MANUAL Catalog #600548 (single kit) #929548 (10-pack kit) Revision B.01 For In Vitro Use Only 600548-12 LIMITED PRODUCT WARRANTY This warranty limits our

More information

Cat. # RR430S RR430A. For Research Use. SYBR Fast qpcr Mix. Product Manual. v201610da

Cat. # RR430S RR430A. For Research Use. SYBR Fast qpcr Mix. Product Manual. v201610da For Research Use SYBR Fast qpcr Mix Product Manual Table of Contents I. Description... 3 II. Principle... 4 III. Components... 5 IV. Storage... 5 V. Features... 6 VI. Precautions... 6 VII. Protocol...

More information

Clostridium difficile Toxin Genes A and B. Real-Time PCR Detection Kit for detection and differentiation of.

Clostridium difficile Toxin Genes A and B. Real-Time PCR Detection Kit for detection and differentiation of. For in vitro Veterinary Diagnostics only. Real-Time PCR Detection Kit for detection and differentiation of Clostridium difficile Toxin Genes A and B www.kylt.eu DIRECTION FOR USE Art. No. 31320 / 31321

More information

EpiQuik Quantitative PCR Fast Kit Base Catalog # P-1029

EpiQuik Quantitative PCR Fast Kit Base Catalog # P-1029 EpiQuik Quantitative PCR Fast Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The EpiQuik Quantitative PCR Fast Kit is designed for quantitative real time analysis of DNA samples

More information

GeneCopoeia TM. All-in-One qpcr Mix For universal quantitative real-time PCR. User Manual

GeneCopoeia TM. All-in-One qpcr Mix For universal quantitative real-time PCR. User Manual GeneCopoeia TM Expressway to Discovery All-in-One qpcr Mix For universal quantitative real-time PCR Cat. No. AOPR-0200 (200 qpcr reactions) Cat. No. AOPR-0600 (600 qpcr reactions) Cat. No. AOPR-1000 (1000

More information

Uses: The Methylamp MS-qPCR Fast Kit is very suitable for quantitative methylation-specific PCR in a fast format using very minute amounts of DNA.

Uses: The Methylamp MS-qPCR Fast Kit is very suitable for quantitative methylation-specific PCR in a fast format using very minute amounts of DNA. Methylamp MS-qPCR Fast Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The Methylamp MS-qPCR Fast Kit is very suitable for quantitative methylation-specific PCR in a fast format

More information

Ah, Lou! There really are differences between us!

Ah, Lou! There really are differences between us! Name Per Ah, Lou! There really are differences between us! Introduction The human genome (the total sum of our genetic makeup) is made up of approximately 6 billion base pairs distributed on 46 chromosomes.

More information

Polyskope 1.0 Multiplex Pathogen Detection Assay

Polyskope 1.0 Multiplex Pathogen Detection Assay Polyskope 1.0 Multiplex Pathogen Detection Assay User Guide Test for the real-time simultaneous PCR detection of E.coli O157 STEC, Salmonella spp. and Listeria monocytogenes in food and environmental samples

More information

qpcr Quantitative PCR or Real-time PCR Gives a measurement of PCR product at end of each cycle real time

qpcr Quantitative PCR or Real-time PCR Gives a measurement of PCR product at end of each cycle real time qpcr qpcr Quantitative PCR or Real-time PCR Gives a measurement of PCR product at end of each cycle real time Differs from endpoint PCR gel on last cycle Used to determines relative amount of template

More information

PRODUCT INFORMATION Thermo Scientific Luminaris Probe Low ROX qpcr Master Mix #K0944 For 5000 rxns Lot Expiry Date Store at -20 C in the dark CERTIFICATE OF ANALYSIS The absence of endo-, exodeoxyribonucleases

More information

For in vitro Veterinary Diagnostics only. Detection Reagents specific for. Chlamydia psittaci Real-Time PCR.

For in vitro Veterinary Diagnostics only. Detection Reagents specific for. Chlamydia psittaci Real-Time PCR. For in vitro Veterinary Diagnostics only. Detection Reagents specific for Chlamydia psittaci Real-Time PCR www.kylt.eu DIRECTION FOR USE Art. No. 31038 / 31039 Kylt Chlamydia psittaci Detection Reagents

More information

Absolute Human Telomere Length Quantification qpcr Assay Kit (AHTLQ) Catalog # reactions

Absolute Human Telomere Length Quantification qpcr Assay Kit (AHTLQ) Catalog # reactions Absolute Human Telomere Length Quantification qpcr Assay Kit (AHTLQ) Catalog #8918 100 reactions Product Description Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect

More information

Absolute Mouse Telomere Length Quantification qpcr Assay Kit (AMTLQ) Catalog #M reactions

Absolute Mouse Telomere Length Quantification qpcr Assay Kit (AMTLQ) Catalog #M reactions Absolute Mouse Telomere Length Quantification qpcr Assay Kit (AMTLQ) Catalog #M8918 100 reactions Product Description Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect

More information

Methods in virus diagnosis PCR techniques

Methods in virus diagnosis PCR techniques Methods in virus diagnosis PCR techniques 450 MBIO PRACTICAL LESSON 5 Molecular Methods Methods based on the detection of viral genome are also commonly known as molecular methods. It is often said that

More information

GreenMasterMix (2X) b i o s c i e n c e. G E N A X X O N b i o s c i e n c e. High ROX (500nM)

GreenMasterMix (2X) b i o s c i e n c e. G E N A X X O N b i o s c i e n c e. High ROX (500nM) G:\products\productflyer\pcr\polymerasen\hotstart\manu_m3052_green_en.docx GreenMasterMix (2) High RO (500nM) qpcr master mix with fluorescence dye and passive reference dye Contact & Technical support

More information

PlantDirect TM Multiplex PCR System

PlantDirect TM Multiplex PCR System PlantDirect TM Multiplex PCR System Technical Manual No. 0178 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 3 IV Shipping and Storage. 3 V Simplified Procedures. 3 VI Detailed

More information

Mastermix 16S Complete, DNA-free

Mastermix 16S Complete, DNA-free Mastermix 16S Complete, DNA-free For the PCR detection and identification of bacteria using universal 16S rdna primers For research use only Cat. No. S-020-0100 Cat. No. S-020-0250 Cat. No. S-020-1000

More information

Event-specific Method for the Quantification of Soybean SYHT0H2 by Real-time PCR. Validated Method

Event-specific Method for the Quantification of Soybean SYHT0H2 by Real-time PCR. Validated Method EUROPEAN COMMISSION JOINT RESEARCH CENTRE Institute for Health and Consumer Protection Molecular Biology and Genomics Unit Event-specific Method for the Quantification of Soybean SYHT0H2 by Real-time PCR

More information

Module1TheBasicsofRealTimePCR Monday, March 19, 2007

Module1TheBasicsofRealTimePCR Monday, March 19, 2007 Objectives Slide notes: Page 1 of 41 Module 1: The Basics Of Real Time PCR Slide notes: Module 1: The Basics of real time PCR Page 2 of 41 Polymerase Chain Reaction Slide notes: Here is a review of PCR,

More information

TECHNICAL BULLETIN. SeqPlex DNA Amplification Kit for use with high throughput sequencing technologies. Catalog Number SEQX Storage Temperature 20 C

TECHNICAL BULLETIN. SeqPlex DNA Amplification Kit for use with high throughput sequencing technologies. Catalog Number SEQX Storage Temperature 20 C SeqPlex DNA Amplification Kit for use with high throughput sequencing technologies Catalog Number SEQX Storage Temperature 20 C TECHNICAL BULLETIN Product Description The SeqPlex DNA Amplification Kit

More information

Premix Ex Taq (Probe qpcr)

Premix Ex Taq (Probe qpcr) For Research Use Premix Ex Taq (Probe qpcr) Product Manual Table of Contents I. Description... 3 II. Principle... 4 III. Components... 5 IV. Materials Required but not Provided... 5 V. Storage... 5 VI.

More information

For feed and food applications only. Kylt Staphylococcus aureus. Real-Time PCR Detection Kit for detection of Staphylococcus aureus.

For feed and food applications only. Kylt Staphylococcus aureus. Real-Time PCR Detection Kit for detection of Staphylococcus aureus. For feed and food applications only. Kylt Staphylococcus aureus Real-Time PCR Detection Kit for detection of Staphylococcus aureus www.kylt.eu DIRECTION FOR USE Art. No. 31009 / 31010 Kylt Staphylococcus

More information

Polymerase Chain Reaction: Application and Practical Primer Probe Design qrt-pcr

Polymerase Chain Reaction: Application and Practical Primer Probe Design qrt-pcr Polymerase Chain Reaction: Application and Practical Primer Probe Design qrt-pcr review Enzyme based DNA amplification Thermal Polymerarase derived from a thermophylic bacterium DNA dependant DNA polymerase

More information

For in vitro Veterinary Diagnostics only. Real-Time RT-PCR Detection Kit for Reticuloendotheliosis Virus

For in vitro Veterinary Diagnostics only. Real-Time RT-PCR Detection Kit for Reticuloendotheliosis Virus For in vitro Veterinary Diagnostics only. Real-Time RT-PCR Detection Kit for Reticuloendotheliosis Virus DIRECTION FOR USE Art. No. 31424 / 31425 Kylt REV Real-Time RT-PCR Detection Kit for Reticuloendotheliosis

More information

Event-specific Method for the Quantification of maize MON by Real-time PCR. Validated Method

Event-specific Method for the Quantification of maize MON by Real-time PCR. Validated Method EUROPEAN COMMISSION JOINT RESEARCH CENTRE Health, Consumers & Reference Materials Food & Feed Compliance Unit Event-specific Method for the Quantification of maize MON 87403 by Real-time PCR Validated

More information

For in vitro Veterinary Diagnostics only. Kylt MHRS Triplex. Real-Time PCR Detection.

For in vitro Veterinary Diagnostics only. Kylt MHRS Triplex. Real-Time PCR Detection. For in vitro Veterinary Diagnostics only. Kylt MHRS Triplex Real-Time PCR Detection www.kylt.eu DIRECTION FOR USE Kylt MHRS Triplex Real-Time PCR Detection A. General Kylt MHRS Triplex products are intended

More information

Real-Time PCR Detection Reagents for Detection of Marek Disease Virus and differentiation between field strains and Rispens vaccine CVI988 strain

Real-Time PCR Detection Reagents for Detection of Marek Disease Virus and differentiation between field strains and Rispens vaccine CVI988 strain For in vitro Veterinary Diagnostics only. Real-Time PCR Detection Reagents for Detection of Marek Disease Virus and differentiation between field strains and Rispens vaccine CVI988 strain www.kylt.eu DIRECTION

More information

10 RXN 50 RXN 500 RXN

10 RXN 50 RXN 500 RXN SeqPlex Enhanced DNA Amplification Kit for use with high throughput sequencing technologies Catalog Number SEQXE Storage Temperature 20 C TECHNICAL BULLETIN Product Description The SeqPlex DNA Amplification

More information

Product Name : Simple mirna Detection Kit

Product Name : Simple mirna Detection Kit Product Name : Simple mirna Detection Kit Code No. : DS700 This product is for research use only Kit Contents This kit provides sufficient reagents to perform 20 reactions for detecting microrna. Components

More information

GenoExplorer mirna qrt-pcr Kit for Catalog # s 2001, 2002, 2003, 2004

GenoExplorer mirna qrt-pcr Kit for Catalog # s 2001, 2002, 2003, 2004 GenoSensor Corporation GenoExplorer mirna qrt-pcr Kit for Catalog # s 2001, 2002, 2003, 2004 Version A April 2007 User Manual Table of Contents Introduction 2 Product System... 2 Kit Components and Storage

More information

Equus caballus Mitochondrial DNA quantification

Equus caballus Mitochondrial DNA quantification PrimerDesign Ltd GMO event quantification Equus caballus Mitochondrial DNA quantification Detection and quantification of Equus caballus mitochondrial DNA by real-time PCR Species Specific Detection detection

More information

SYBR Advantage qpcr Premix. User Manual

SYBR Advantage qpcr Premix. User Manual User Manual SYBR Advantage qpcr Premix User Manual United States/Canada 800.66.566 Asia Pacific +.650.99.7300 Europe +33.(0).3904.6880 Japan +8.(0)77.543.66 Clontech Laboratories, Inc. A Takara Bio Company

More information

Accurately and reproducibly quantify as little as 20 fg of bacterial DNA using real-time PCR.

Accurately and reproducibly quantify as little as 20 fg of bacterial DNA using real-time PCR. INSTRUCTION MANUAL Femto Bacterial DNA Quantification Kit Catalog No. E2006 Highlights Accurately and reproducibly quantify as little as 20 fg of bacterial DNA using real-time PCR. High specificity and

More information

foodproof GMO RR 2 Yield Soya Quantification Kit

foodproof GMO RR 2 Yield Soya Quantification Kit For GMO Testing purposes. FOR IN VITRO USE ONLY. foodproof GMO RR 2 Yield Soya Quantification Kit - 5 Nuclease - Version 2, March 2017 PCR kit for the quantitative detection of genetically modified soya

More information

ZIKA Virus (ZIKAV) Real Time RT-PCR Kit

ZIKA Virus (ZIKAV) Real Time RT-PCR Kit ZIKA Virus (ZIKAV) Real Time RT-PCR Kit Product Catalogue No.: BP801 Storage temperature: -15 C to -25 C For In Vitro Diagnostic Use for Research and Investigational Use Only Instruction for use 1. Intended

More information

Brilliant III Ultra-Fast SYBR Green QPCR Master Mix

Brilliant III Ultra-Fast SYBR Green QPCR Master Mix Brilliant III Ultra-Fast SYBR Green QPCR Master Mix Instruction Manual Catalog #600882 (single kit) #600883 (10-pack kit) Revision D0 Research Use Only. Not for Use in Diagnostic Procedures. 600882-12

More information

PRODUCT INFORMATION Thermo Scientific Luminaris Color HiGreen Low ROX qpcr Master Mix #K0373 For 1250 rxns Lot Exp. Store at -20 C in the dark CERTIFICATE OF ANALYSIS The absence of endo-, exodeoxyribonucleases

More information

TECHNICAL BULLETIN. SYBR Green JumpStart Taq ReadyMix without MgCl 2. Catalog Number S5193 Storage Temperature 20 C

TECHNICAL BULLETIN. SYBR Green JumpStart Taq ReadyMix without MgCl 2. Catalog Number S5193 Storage Temperature 20 C SYBR Green JumpStart Taq ReadyMix without MgCl 2 Catalog Number S5193 Storage Temperature 20 C TECHNICAL BULLETIN Product Description SYBR Green JumpStart Taq ReadyMix without MgCl 2 combines JumpStart

More information

Quantitative Real time PCR. Only for teaching purposes - not for reproduction or sale

Quantitative Real time PCR. Only for teaching purposes - not for reproduction or sale Quantitative Real time PCR PCR reaction conventional versus real time PCR real time PCR principles threshold cycle C T efficiency relative quantification reference genes primers detection chemistry GLP

More information

TECHNICAL BULLETIN. SYBR Green Extract-N-Amp Tissue PCR Kit Catalog Numbers XNATG and XNATRG

TECHNICAL BULLETIN. SYBR Green Extract-N-Amp Tissue PCR Kit Catalog Numbers XNATG and XNATRG SYBR Green Extract-N-Amp Tissue PCR Kit Catalog Numbers XNATG and XNATRG TECHNICAL BULLETIN Product Description The SYBR Green Extract-N-Amp Tissue PCR Kit contains all the reagents needed for rapid extraction,

More information

DUPLICα REAL TIME SALMONELLA

DUPLICα REAL TIME SALMONELLA Instruction Manual DUPLICα REAL TIME SALMONELLA Code EBR004032 Format 32 tests For in Vitro Use Only Store the kit components at +2-8 C Euroclone Life Sciences: Via Figino, 20/22-20016 Pero (MI) ITALY

More information

HBV Quantitative & YMDD Mutation Real Time PCR Kit

HBV Quantitative & YMDD Mutation Real Time PCR Kit Revision No.: ZJ0002 Issue Date: Aug 7 th, 2008 HBV Quantitative & YMDD Mutation Real Time PCR Kit Cat. No.: HD-0003-01 For Use with LightCycler 1.0/LightCycler2.0/LightCycler480 (Roche) Real Time PCR

More information

Relative Mouse Telomere Length Quantification qpcr Assay Kit (RMTLQ) Catalog #M reactions

Relative Mouse Telomere Length Quantification qpcr Assay Kit (RMTLQ) Catalog #M reactions Relative Mouse Telomere Length Quantification qpcr Assay Kit (RMTLQ) Catalog #M8908 100 reactions Product Description Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect

More information

PRODUCT INFORMATION Thermo Scientific Luminaris Color Probe qpcr Master Mix #K0354 For 5000 rxns Lot Exp. Store at -20 C in the dark CERTIFICATE OF ANALYSIS The absence of endo-, exodeoxyribonucleases

More information

HBV Quantitative & YMDD Mutation Real Time PCR Kit

HBV Quantitative & YMDD Mutation Real Time PCR Kit Revision No.: ZJ0002 Issue Date: Aug 7 th, 2008 HBV Quantitative & YMDD Mutation Real Time PCR Kit Cat. No.: HD-0003-02 For Use with LightCycler2.0/LightCycler480 (Roche) Real Time PCR Systems For In Vitro

More information

Roche Molecular Biochemicals Technical Note No. LC 10/2000

Roche Molecular Biochemicals Technical Note No. LC 10/2000 Roche Molecular Biochemicals Technical Note No. LC 10/2000 LightCycler Overview of LightCycler Quantification Methods 1. General Introduction Introduction Content Definitions This Technical Note will introduce

More information

Hy-Fy High Fidelity Mix (x2)

Hy-Fy High Fidelity Mix (x2) Hy-Fy High Fidelity Mix (x2) #EZ-2021 1ml, 100rxn of 20μl Contents: 2X High Fidelity Mix 1ml Nuclease-free water 1ml Store at -20 C Shelf life: 2 years Description Hy-Fy High Fidelity Mix (x2) is a premixed,

More information

Stratagene QPCR Mouse Reference Total RNA

Stratagene QPCR Mouse Reference Total RNA Stratagene QPCR Mouse Reference Total RNA Instruction Manual Catalog #750600 Revision B Research Use Only. Not for Use in Diagnostic Procedures. 750600-12 LIMITED PRODUCT WARRANTY This warranty limits

More information

SYBR Premix DimerEraser (Perfect Real Time)

SYBR Premix DimerEraser (Perfect Real Time) Cat. # RR091A or Research Use SYBR Premix DimerEraser (Perfect Real Time) Product Manual Table of Contents I. Description... 3 II. Principle... 3 III. Components... 4 IV. Storage... 5 V. eatures... 5 VI.

More information

Copy number standard curve. for absolute quantification by real-time PCR

Copy number standard curve. for absolute quantification by real-time PCR Copy number standard curve for absolute quantification by real-time PCR Kit contents Positive control template for standard curve (RED) Template preparation buffer (YELLOW) RNase/DNase free water (WHITE)

More information

TQ RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 2 TQ RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 5

TQ RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 2 TQ RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 5 www.smobio.com Product Information ExcelTaq series 2X Q-PCR Master Mix (SYBR, no ROX) TQ1100 200 RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 2 TQ1101 500 RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x

More information

Escherichia coli_o104: H4

Escherichia coli_o104: H4 PCRmax Ltd TM qpcr test Escherichia coli_o104: H4 150 tests For general laboratory and research use only 1 Introduction to Escherichia coli_o104:h4 2 Specificity MAX MIN The PCR Max qpcr Kit for Escherichia

More information

#K0262 For 1000 reactions of 25 µl Lot Exp.. Store at -20 C in the dark. V

#K0262 For 1000 reactions of 25 µl Lot Exp.. Store at -20 C in the dark. V PRODUCT INFORMATION Thermo Scientific Maxima Probe qpcr Master Mix (2X), ROX Solution provided #K0262 For 1000 reactions of 25 µl Lot Exp.. Store at -20 C in the dark. V CERTIFICATE OF ANALYSIS The absence

More information

HPV 16&18 Real Time PCR Kit

HPV 16&18 Real Time PCR Kit HPV 16&18 Real Time PCR Kit Cat. No.: TD-0030-02 For use with ABI Prism 7000/7300/7500/7900; Smart CyclerII; icycler iq 4/iQ 5; Rotor Gene 2000/3000; Mx3000P/3005P; MJ-Option2/Chromo4 real time PCR systems

More information

QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I) (Last Revised: April, 2007)

QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I) (Last Revised: April, 2007) QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I) (Last Revised: April, 007) Please contact Center for Plant Genomics (CPG) facility manager Hailing Jin (hljin@iastate.edu) regarding questions or corrections.

More information