Consumables. For Capillary Electrophoresis & Fragment Analysis

Transcription

1 Consumables For Capillary Electrophoresis & Fragment Analysis

2 Content NanoPOP Genetic Analyzer Polymers Capillary Electrophoresis & Fragment Analysis BrightDye Terminator Cycle Sequencing Kit FastGene DyeTerminator Removal kit D-Pure DyeTerminator Removal Kit Fluorescent DNA Size Standards 310 Capillaries AmpliClean Magnetic Beadbased PCR Clean-up Alpaqua 24 & 96-well Magnet Plates

3 NanoPOP Genetic Analyzer Polymers Save up to 50% on polymers for your ABI Genetic Analyzer without compromise! High resolution & performance Better stability & shelf life Compatible with all ABI Genetic Analyzers NanoPOP-4, NanoPOP-6 & NanoPOP-7 are separation matrixes, newly formulated from nanoparticles. These new matrixes, designed for ABI genetic analyzers, have excellent coating and separating ability with all different applications. All NanoPOP polymers can be used without any change in run protocols, conditions or spectral calibrations. Developed with the latest polymer chemistry technology, NanoPOP has a increased stability and shelf life. Compatability NanoPOP-4 is compatible with CE 10X running buffer. Nano- POP-7 and NanoPOP-6 are compatible with NanoPOP 10X running buffer. CE 10X running buffer is also compatible with ABI s POP-4, POP-6 & POP-7. NanoPOP-7 for 3500 Genetic Analyzer NimaGen now also offers the NanoPOP-7 Polymer and 1x NanoPOP Running Buffer for the 3500 series Genetic Analyzers. The polymer is offered in pouches, which are directly attachable to the instrument as a research use only product. The NanoPOP-7 gives you the advantage of increased on-instrument lifespan of 14 days! The NanoPOP Running Buffer for the 3500 is offered original containers. Provided are 2 bottles of 270 ml for a total of 4 ers. Included are self-adhesive RFID tags (4x CBC abnd 4x ABC) with a programmed on-instrument maximum lifespan of 14 days. NanoPOP-4 (3130/3130XL) 5 ml NP4-100 NanoPOP-4 (3130/3130XL) 10 ml NP4-101 NanoPOP-4 (310/3100) 5 ml NP4-120 NanoPOP-4 (310/3100) 10 ml NP4-121 NanoPOP-6 (3130/3130XL) 5 ml NP6-100 NanoPOP-6 (3130/3130XL) 10 ml NP6-101 NanoPOP-6 (310/3100) 5 ml NP6-120 NanoPOP-6 (310/3100) 10 ml NP6-121 NanoPOP-7 (3130/3130XL) 5 ml NP7-100 NanoPOP-7 (3130/3130XL) 10 ml NP7-101 NanoPOP-7 (3730/3730XL) 28 ml NP7-300 NanoPOP-7 (3730/3730XL) 10 x 28 ml NP7-301 NanoPOP-7 (3500) 384 rxns NP7-135 NanoPOP-7 (3500) 960 rxns NP7-235 NanoPOP 10X running buffer 100 ml NPB-100 NanoPOP 10X running buffer 500 ml NPB-500 NanoPOP 1X running buffer for x 270 ml NPB-135 CE 10X running buffer 100 ml RBUF-100 CE 10X running buffer 500 ml RBUF-500

4 BrightDye Terminator Cycle Sequencing Kit Capillary Electrophoresis & Fragment Analysis No need for any change in protocol, settings or analysis parameters High Performance for sequencing of GT-rich regions Uniform peak hights and optimal signal balance Reduce your costs Robust design for high success rates The BrightDye Terminator Cycle Sequencing Kit is a reagent used for labeling the dideoxynucleoside triphosphates using the Sanger sequencing method. This facili- products of DNA sequencing reactions are separated by gel or by capillary electrophoresis. Bright- Dye Terminator Cycle Sequencing Kit is compatible with v3.1 sequencing chemistry setting. Ideal for de novo sequencing, re- product, Plasmid, Cosmid and BAC templates. Guaranteed Performance All BrightDye Terminator sequencing reagents are tested twice for quality. First for correct formulation and secondly for consistent and reliable performance on sequencing systems. Applications - Ideal for PCR products and de novo sequencing - plates - Long-read sequencing - Sequencing accross all template types (plasmid, PCR Products, BACs and cosmids) - Excellent mixed-base detection - Sequencing short PCR products using rapid electrophoresis run modules Platforms 310, 3100, 3130, 3130XL, 3500, 3500XL, 3730 & 3730XL Genetic Analyzers. Content All kits include BrightDye Terminator ready reaction premix, 5X sequencing buffer, pgem control and -21M13 primer. BrightDye Terminator Cycle Sequencing Kit 10 rxn, contains 80 μl 2.5X Ready Reaction Premix BDT3-50 BrightDye Terminator Cycle Sequencing Kit 100 rxn, contains 800 μl 2.5X Ready Reaction Premix BDT3-100 BrightDye Terminator Cycle Sequencing Kit 1000 rxn, contains 8 ML 2.5X Ready Reaction Premix BDT3-200 BrightDye Terminator Cycle Sequencing Kit 5000 rxn, contains 40 ML 2.5X Ready Reaction Premix BDT3-300 BrightDye Terminator Cycle Sequencing Kit rxn, contains 200 ML 2.5X Ready Reaction Premix BDT3-400

5 Terminator on a 3730XL Genetic Analyzer. FastGene DyeTerminator Removal Kit Long shelf life - storage at room temperature FastGene DyeTerminator Removal Kit removes unincorperated DyeTerminator molecules from sequencing samples using an ef- the matrix is mixed with resuspension buffer, then the equilibrated matrix is transferred to the spin down, up to 20 μl of the sequencing reaction mix can be pu- nal centrifugation step. All kits can be stored at room temperature. The maximum sample volume is 20 μl sequencing reaction. The obtained DNA recovery is over 90%. Nippon Fastgene Dye Terminator Removal kit 50 preps FG-9411 *Only available in the Benelux, for other countries please contact us.

6 D-pure DyeTerminator Removal Kit by magnetic beads Capillary Electrophoresis & Fragment Analysis Proprietary formula No C-degradation Very Competitive prices D-pure DyeTerminator removal kit is a magnetic bead-based high-throughput purification method for DNA sequencing reactions. The kit consists of a magnetic bead solution and an elution buffer. Each component has been optimized for removing salts and unincorporated DyeTerminators from DNA sequencing reac- products are more stable than the leading competitor s magnetic beads. The system can be easily adapted in your current liquid handling system. Please visit our website for the Hamilton application note, describing how to use D-pure in an automated liquid handling system. D-pure kit D-pure kit D-pure kit 5 ml (total volume) 500 preps (96-well format) 50 ml (total volume) 5000 preps (96-well format) 500 ml (total volume) preps (96-well format) DP-005 DP-050 DP-500

7 Fluorescent DNA Size Standards Assign sizes to DNA fragments Red DNA Size Standard (GeneScan 500 ROX alternative) Orange DNA Size Standard (GeneScan alternative) Double Peak DNA Size Standard for monitoring resolution performance of the installed capillary arrays MCLAB s DNA Size Standard products are internal lane standards that are intended to be used in assigning sizes to DNA fragments ments. The common applications include genotyping and DNA fragment Analysis. Each of these standards consists of 15 DNA fragments, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 bp. The fragments are single-stranded with carboxy-x-rhodamine (ROX orange dye. Size fragments are evenly distributed and can be used for very accurate size calling. Compatible with ABI Genetic Analyzers: 310, 3100, 3130/3130XL, 3500 and 3730/3730XL. Red DNA Size Standard This size standard has been adapted to DNA fragment analysis software, e.g. GeneMapper and Peak Scanner by using the same parameters as ABI s GS 500 ROX Size Standard. Orange DNA Size Standard The orange DNA size standard can be used with the same settings as for ABI s GS size standard. Double Peak DNA Size Standard This size standard is similar to the red DNA size standard, except every fragment is accompanied by an one-base-plus band. For example, 50 becomes 50 and 51, 75 becomes 75 and 76, etc. This single base separation is very useful for monitoring the performance of the installed capillary arrays. Red DNA Size Standard 800 analysis (400μl) DSMR-100 Orange DNA Size Standard 800 analysis (400μl) DSMO-100 Double Peak DNA Size Standard 800 analysis (400μl) DSMD Capillaries 310 Capillaries are bare fused silica capillary tubing s, 47cm x read). They are designed and optimized for fragment analysis or sequencing applications on 310 Genetic Analyzer. The capillaries have a low fluorescent background and are very reliable (>100 runs/capillary). 310 Capillary 47 cm, 5 pieces CAP Capillary 61 cm, 2 pieces CAP-61

8 AmpliClean Magnetic Beadbased PCR Clean-up Recover amplicons >100 bp with consistency and high reproducibility dimers, salts and dntp s Highly stable PCR products at 4 C. Easy integration with automated liquid handling system for handsoff work- 1. PCR product or library. 2. Add AmpliClean to PCR product. 3. Place plate on the magnet, discard supernatant. 4. Ethanol wash. 5. Add elution buffer. 6. Transfer eluate to new tube. AmpliClean Magnetic Beadbased PCR Clean-up delivers su- out salt carryover. It utilizes Solid Phaze Reversible Immobilization magnetic bead-based technology of PCR products. This technique is based on magnetic separation. Therefore, it requires no centrifu- l AmpliClean selectively binds PCR amplicons of 100bp and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes are removed by using a simple washing procedure. The pure and free of contaminants.

9 Next Generation Sequencing Applications Ampi- Clean can be used in applications, such as: Sanger Sequencing Next-Gen Sequencing Library preparation size selection Fragment Analysis system recovers double stranded or single stranded PCR products >100bp. This is done in a consistend and highly reproducible manner. The recovery rate for small and large amplicon sizes is higher clean-up methods as can be seen DNA the recovery rate is slightly less than for double stranded DNA. liquid handling platforms Due to the use of magnetic separation, AmpliClean requires no - procedure can be easily incorperated in automated liquid handling platforms equiped with a magnetic plate. The AmpiClean PCR pu- or automated in 96- and 384-well formats. 5 ml AC ml AC ml AC-500 Next Generation Sequencing Alpaqua Integrated spring cushion technology for complete liquid removal without tip occlusion Ring magnets provide for rapid bead capture and allow complete removal of supernatants, wash buffers and eluates from the well bottom Optimized for robotic access Compatible with common magnetic bead chemistries and protocols for Optimized for all common liquid handling systems, such as: Hamilton, Beckmann, Tecan and Perkin-Elmer

10 96R Ring Magnet Plate The 96R Ring Magnetic Plate contains 96 ring-shaped NdFeB magnets and intergrated spring cushion technology. This magnet plate is ideal for round-bottom microplates (e.g. Corning Costar 3795 series) and separation volumes up to 350 μl in 96-well PCR plates (skirted or non-skirted). 96S Super Magnet Plate The 96S Super magnet plate with integrated spring cushion technology contains 96 ring-shaped NdFeB magnets. These magnets are stronger than the ones in the 96R Ring Magnet Plates. This magnet plate can be used for large volume separations up to 1 ml. It also can be used for faster separations in smaller volumes. This magnet plate contains our strongest magnet and can perform your most challenging separations 40% faster. Ideal for use with viscous samples like blood and serum. This magnet plate is compatible with virtually any 96-well microplate, including Round-Bottom, Flat-Bottom, V-Bottom, Deep-Well or PCR plates. Furthermore, the elevated base of the magnet plate ensures full compatibility with virtually any automated liquid handling platform, including those with deep plate nests. We offers the only 24-well magnet plate with integrated spring cushion technology and NdFeB ring-based magnets. This combination results in maximum bead capture and complete aspiration of wash solutions and supernatants, while eliminating costly tip crashes. The MagPlate 24 is optimized for robotic access and comply with SBS footprint standards for maximum liquid handler and stacker compatibility. They are designed for use with 24-well large volume (10ml) Whatman UniPlates 96R Ring Magnet Plate Separation volumes up to 350μl in 96-well PCR plates (skirted or nonskirted) A S Super Ring Magnet Plate Large volume separations up to 2 ml A Compatible with virtually any 96-well microplate, including Round-Bottom, Flat-Bottom, V-Bottom, Deep-Well or PCR plates A well Magnet Plate 24-well large volume (10ml) A000270

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12 www nimagen com Capillary Electrophoresis & Fragment Analysis FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES NimaGen, part of Biolegio Nijmegen, The Netherlands. All rights reserved. NanoPOP, NanoPOP-4, NanoPOP-6, NanoPOP-7 are trademarks of Molecular Cloning Lab, Inc. in the U.S. BrightDye is a registered trademark of ICloning, Inc. China. Per informazioni tecniche FastGene e commerciali is a registered potete trademark rivolgervi of al Nippon distributore Genetics esclusivo Europe GmbH. per l Italia: D-pure is a trademark of NimaGen, part of Biolegio Nijmegen, The Netherlands. All other trademarks are the sole property of their respective owners. Servizio Clienti Via Cadore, GENZANO di Roma RM - Italy - Tel.: (+39) Fax.: (+39) servizioclienti@resnovaweb.it Per invio ordini: ordini@resnovaweb.it PEC-mail: resnova@pec.it S.r.l. Sito WEB: