Expression Array System
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1 Integrated Science for Gene Expression Applied Biosystems Expression Array System Expression Array System
2 SEE MORE GENES
3 The most complete, most sensitive system for whole genome expression analysis. The Applied Biosystems Expression Array System is a dramatic improvement over current technology for determining differential gene expression, delivering unambiguous, high-quality results that drive increased research productivity. The system s advanced design and novel chemiluminescence chemistry allow you to detect expression events at lower levels and on more genes than ever before using a minimal amount of sample. With an integrated workflow on a single microarray built on today s most complete dataset you can survey and measure gene expression across your choice of either human or mouse genomes. Using a wealth of related annotations, you can isolate individual genes for further analysis. And with links to convenient TaqMan Gene Expression Assays matched to the arrays, you can easily validate your microarray results and move your research forward faster. The Expression Array System is part of Applied Biosystems iscience gene expression solution a complete, truly integrated platform for quantitative gene expression research. The system includes Applied Biosystems advanced Genome Survey Microarray, 1700 Chemiluminescent Microarray Analyzer, high-performance labeling and detection reagent kits, comprehensive dataset of genomic information, and powerful data management software.
4 See more genes. Greater sensitivity means the ability to identify more genes, even those with very low expression levels. An advanced microarray design and novel chemiluminescence signaling chemistry contribute to the enhanced performance of the system. Chemiluminescence technology requires no excitation and results in very low background noise; it dramatically improves signal-to-noise values, and enables low-level detection not achievable using other array configurations. Applied Biosystem Genome Survey Microarray In addition to quality-assured probes for targeted genes, each Genome Survey Microarray includes extensive control features for setup, analysis, diagnostics, and monitoring of chemiluminescent reactions, hybridization, and labeling performance. Corresponding TaqMan Gene Expression Assays are available for each of the targeted genes. In addition, the Genome Survey Microarray uses 60-mer DNA probes for the optimum combination of sensitivity and specificity. This permits the single-base hybridization specificity of shorter oligonucleotides, without the decreased sensitivity commonly encountered when using shorter probes. Immobilized Oligo Sensitivity comparison: typical chemiluminescence vs. fluorescence. Label Method Detection (Substrate) Limit (mol) Hybridized DIG Labeled cdna or crna Fluorescein dye Fluorescence 2 x Rhodamine dye Fluorescence 5 x Horse Radish Peroxidase Colorimetric 1 x Alkaline Phosphatase Colorimetric 2 x Alkaline Phosphatase Chemiluminescence 2 x DIG Antibody alkaline phosphatase conjugate High signal strength and absence of background noise in chemiluminescent technology allows detection of extremely low levels of gene expression, compared with alternative fluorescence-based techniques. Enhancer interacts with the activated chemiluminescent substrate which in turn produces light!
5 Use less sample. Greater sensitivity also means the ability to use less sample compared to standard approaches for whole genome analysis. A novel chemiluminescence signaling chemistry provides detection sensitivity at the femtomolar level, enabling the Expression Array System to distinguish expression levels of less than one copy per cell. Applied Biosystems amplified labeling kits (based on industry-standard Eberwine protocols) and detection kit let you interrogate the complete genome from just 500 ng (sample quality dependent) of total RNA, allowing sparing use of valuable tissue and cell culture samples. For those applications that are not sample-limited, a convenient, non-amplifying labeling chemistry is also available. Powerful database management built into the system. A powerful Oracle relational database is included with the Expression Array System to help you manage the millions of data points produced over the course of a microarray gene expression project. The database can easily scale up to handle the ever-increasing data management requirements of busy core facilities, and supports industry-standard microarray data files, as well as the Expression Array System s innovative multi-project, multi-user data organization and security features. Applied Biosystems chemiluminescence labeling kits for RT and RT-in vitro transcription (RT-IVT) optimize incorporation of label into your target sequence. Designed specifically for the Expression Array System, these kits accommodate a wide range of sample sizes, from just 500 ng to 40 µg of total RNA. The Applied Biosystems Chemiluminescence Detection Kit uses robust chemistry to report the hybridization of labeled cdna or crna to probes on the microarray. TaqMan Gene Expression Assays Once you identify genes that are differentially expressed, it s easy to move your research to conclusion using TaqMan Gene Expression Assays and Real-time PCR Systems from Applied Biosystems. Microarray results link to TaqMan -based assays for every gene, so you can validate your microarray results, obtain absolute quantitation of transcript production, and investigate the role of alternative splice variations.
6 Expression analysis of the most complete genome. The microarray design of the Expression Array System is based upon a complete, accurate transcript library of the human or mouse genome the most comprehensive and most thoroughly annotated transcript data sets available. Each probe is associated with a gene sequence built on unique Celera data and validated public RefSeqs that have been rigorously curated by an expert scientific team. The Expression Array System includes a continually updated, searchable Oracle database that enables annotation of microarray data with the latest genomic information. Resident software allows queries of the gene expression data based on this annotation to produce a filtered gene list. This example shows microarray data filtered against the Panther database s Molecular Function and Biological Process Classifications: [ie: cell cycle and kinase], detectable genes (S/N > 3), and data quality (signal flags < 100).
7 Use less sample.
8 Complete, integrated solutions for gene expression research. Whether you re screening a few genes over a few samples or many thousands over a large population, Applied Biosystems offers integrated and integratable solutions that will give you the precise, reproducible results your research requires. Advanced whole genome microarray system Quantitative real-time PCR instruments Celera Discovery System TaqMan Gene Expression Assays and Custom TaqMan Assays Quality-assured Custom TaqMan Probes Optimized reagents and consumables The world s most comprehensive library of human and mouse genome sequence data Advanced analysis and data management software Time-saving sample preparation systems Professional laboratory services Want to see more genes and use less sample, too? For more information about the Applied Biosystems Expression Array System, or any of our complete, integrated solutions for gene expression research, please visit iscience. To better understand the complex interaction of biological processes, life scientists are developing revolutionary approaches to discovery that unite technology, informatics, and traditional laboratory research. In partnership with our customers, Applied Biosystems provides the innovative products, services, and knowledge resources that make this new, Integrated Science possible. Worldwide Sales Offices Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches 150 countries on six continents. For international office locations, please call the division headquarters or refer to our Web site at Applera is committed to providing the world s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Headquarters 850 Lincoln Centre Drive Foster City, CA USA Phone: Toll Free: Fax: For Research Use Only. Not for use in diagnostic procedures. Certain Applied Biosystems PCR reagents are developed and manufactured by Roche Molecular Systems, Inc. The PCR process and 5' nuclease process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. TaqMan is a registered trademark of Roche Molecular Systems, Inc. AB (Design), Applera, Assays-on-Demand, Celera, iscience, and iscience (Design) are trademarks and Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries. All other trademarks are the property of their respective owners. Information is subject to change without notice Applied Biosystems. All rights reserved. Printed in the USA, 03/04, P+s Publication 130BR01-01
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