Product Datasheet. HLA-DR Antibody (L243) NB Unit Size: 0.1 ml

Transcription

1 Product Datasheet HLA-DR Antibody (L243) NB Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 26 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/18/2016 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB HLA-DR Antibody (L243) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Target Molecular Weight 0.1 ml 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal L % Sodium Azide IgG2a Kappa Protein A purified PBS 28 kda Page 1 of 6 v.20.1 Updated 8/18/2016 Product Description Host Mouse Gene ID 3122 Gene Symbol Species Reactivity Notes HLA-DRA Human, Baboon, Canine, Primate Human. Predicted cross-reactivity with Chimpanzee, Baboon, Cynomolgus, Rhesus, Marmoset, Tamarin, Squirrel Monkey and Canine. Immunogen Human lymphoblastoid cell line (RPMI 8866). Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Flow Cytometry, Flow (Cell Surface), Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, CyTOF-ready Western Blot 1:100-1:2000, Flow Cytometry < 0.5 ug/10^6 cells, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:10-1:500, Flow (Cell Surface), CyTOF-ready It is recommended that the reagent be titrated for optimal performance for each application. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

3 Images Western Blot: HLA-DR Antibody (L243) [NB ] - Western blot analysis of human spleen (A) and human tonsil (B) tissue using HLA- DR antibody at a concentration of 2 ug/ml. Page 2 of 6 v.20.1 Updated 8/18/2016 Immunohistochemistry-Paraffin: HLA-DR Antibody (L243) [NB ] - IHC staining of HLA-DR in human spleen using DAB with hematoxylin counterstain. using the DyLight 405 conjugate of NB Staining of HLA-DR in human PBMCs using anti-hla-dr antibody. Image from verified customer review. Flow Cytometry: HLA-DR Antibody (L243) [NB ] - Human peripheral blood lymphocytes stained with L243 PE

4 Flow (Cell Surface): HLA-DR Antibody (L243) [NB ] - Analysis using the Azide Free version of NB Staining of HLA-DR expression by 5x10^5 BDCM cells using 0.5 ug of (red) and 0.5 ug of isotype control (green). This antibody and an anti-mouse IgG PE conjugated secondary this antibody were used for t Page 3 of 6 v.20.1 Updated 8/18/2016 using the PerCP/Cy5.5 conjugate of NB Staining of HLA-DR expression by 5x10^5 human PBMC using 10 ul (0.25 ug) of this antibody. Cell surface flow kit was used for this test. Left panel shows the monocyte gate, right panel shows the lymphocyte gate. using the Alexa Fluor (R) 700 conjugate of NB Staining of HLA-DR protein in human PBMCs using HLA-DR antibody [clone L243; conjugated to Alexa Fluor (R) 700] at a dilution of 1:100 with 25 minutes incubation at 4C in 2% human serum, 0.5 mm EDTA in DPBS (Image submitted via Verified Customer Review). using the Biotin conjugate of NB Staining of Human peripheral blood lymphocytes with biotinylated L243, followed by Sav- PE.

5 using the FITC conjugate of NB Staining of Human peripheral blood lymphocytes with L243 FITC. Page 4 of 6 v.20.1 Updated 8/18/2016

6 Publications Petersen SH Odintsova E Haigh TA et al. The role of tetraspanin CD63 in antigen presentation via MHC class II. Eur J Immunol Sep 1 [PMID: ] (EM, Human) Apps R Murphy SP Fernando R et al. Human leucocyte antigen (HLA) expression of primary trophoblast cells and placental cell lines, determined using single antigen beads to characterize allotype specificities of anti-hla antibodies. Immunology May 1 [PMID: ] (FLOW, Human) Ivanov A Beers SA Walshe CA et al. Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest Aug 1 [PMID: ] (Func, In vitro, Human) Setterblad N Garban F Weigl R et al. Extracorporeal photophoresis increases sensitivity of monocytes from patients with graft-versus-host disease to HLA-DR-mediated cell death. Transfusion 2008 Jan 1 [PMID: ] (Func, Human) Details: HLA DRB1 antibody (clone L243) was used at 10ug/ml conncentration for induction of cell death ex vivo on peripheral blood mononuclear cells /PBMNCs that were isolated from graft-versus-host disease/gvhd patients. Wahlstrom J Dengjel J Persson B et al. Identification of HLA-DR-bound peptides presented by human bronchoalveolar lavage cells in sarcoidosis. J Clin Invest Nov 1 [PMID: ] Ovsyannikova IG Johnson KL Naylor S Poland GA. Identification of HLA-DRB1-bound self-peptides following measles virus infection. J Immunol Methods Feb 1 [PMID: ] (FLOW, Human) Pashine A Busch R Belmares MP et al. Interaction of HLA-DR with an acidic face of HLA-DM disrupts sequencedependent interactions with peptides. Immunity 2003 Aug 1 [PMID: ] (FLOW) Gehring AJ Rojas RE Canaday DH et al. The Mycobacterium tuberculosis 19-kilodalton lipoprotein inhibits gamma interferon-regulated HLA-DR and Fc gamma R1 on human macrophages through Toll-like receptor 2. Infect Immun 2003 Aug 1 [PMID: ] (B/N, Human) Hegde NR Tomazin RA Wisner TW et al. Inhibition of HLA-DR assembly, transport, and loading by human cytomegalovirus glycoprotein US3: a novel mechanism for evading major histocompatibility complex class II antigen presentation. J Virol Nov 1 [PMID: ] (FLOW, Human) Details: HLA DRB1 antibody (clone L243) was used for FLOW analysis of His16 cells (derived by stable transfection of the human astroglioma cells U373-MG with the human class II transactivator CIITA) that were infected with Adtet-trans (120 PFU/cell) or AdtetUS2 and Adtet-trans (100 and 20 PFU/cell, respectively) for 18 hours. The antibody was used at 1:1000 dilution with 1 hour 4C incubation and detected using goat anti-mouse FITC conjugated secondary antibody on FACSCalibur flow cytometer. Patil NS Pashine A Belmares MP et al. Rheumatoid arthritis (RA)-associated HLA-DR alleles form less stable complexes with class II-associated invariant chain peptide than non-ra-associated HLA-DR alleles. J Immunol 2001 Dec 15 [PMID: ] (IP, FLOW) Kostelny SA Link BK Tso JY et al. Humanization and characterization of the anti-hla-dr antibody 1D10. Int J Cancer 2001 Aug 15 [PMID: ] (ELISA, Func, Human) Details: pan-hla-dr antibody clone L243 was used for induction of apoptosis in cultured Raji cells (Figure 8). Clone L243 of anti-hla-dr was used as developing antibody with Hu1D10 (capturing antibody) for ELISA assays on the Raji cell lysate. Doebele RC Busch R Scott HM et al. Determination of the HLA-DM interaction site on HLA-DR molecules. Immunity 2000 Oct 1 [PMID: ] (FLOW, WB, ELISA) More publications at Page 5 of 6 v.20.1 Updated 8/18/2016

7 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP mg Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG2a Kappa Light Chain Isotype Control (M2a) NB V HLA-DR Antibody (L243) [DyLight 405] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: