Product Datasheet. CD31/PECAM-1 Antibody (JC/70A) NB ml. Unit Size: 0.1 ml

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1 Product Datasheet CD31/PECAM-1 Antibody (JC/70A) NB ml Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. After opening store under sterile conditions. Reviews: 3 Publications: 17 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 3/12/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB ml CD31/PECAM-1 Antibody (JC/70A) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Product Description Host 0.1 ml 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. After opening store under sterile conditions. Monoclonal JC/70A 0.02% Sodium Azide IgG1 Kappa Protein G purified PBS Mouse Gene ID 5175 Gene Symbol Species Reactivity Notes Immunogen PECAM1 Human, Feline, Rabbit Human and rabbit. Rabbit data from customer review. Page 1 of 7 v.20.1 Updated 3/12/2018 Membrane preparation of a spleen from a patient with hairy cell leukemia. Product Application Details Applications Recommended Dilutions Application Notes Western Blot, ELISA, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry Western Blot 1:100-1:500, Flow Cytometry 1:100-1:250, ELISA, Immunohistochemistry 1:10-1:25, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:25, Immunohistochemistry-Frozen, Flow (Cell Surface) 1:100-1:250, Immunocytochemistry 1:25 This CD31/PECAM1 Antibody (JC/70A) is useful for IHC on paraffin-embedded sections, Flow cytometry and Western blot. It reacts with an ~100 kda glycoprotein expressed by endothelial cells and ~130 kda glycoprotein present in platelets. It stains endothelial cells in normal as well as malignant tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (ph 6.0) is recommended. Use in ELISA reported in scientific literature (PMID ). Rabbit reactivity is reported in a publication (PMID: ) which cites the use of this monoclonal antibody, clone JC/70A.

3 Images Immunocytochemistry: CD31/PECAM-1 Antibody (JC/70A) [NB ] - Imaging of Feline Endothelial cells with antibody dilutionof 1:25. CD31/PECAM-1 (Red), DAPI (Blue). This image was submitted via customer Review. Page 2 of 7 v.20.1 Updated 3/12/2018 Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB ] - IHC analysis of CD31/PECAM1 in human spleen using DAB with hematoxylin counterstain. Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB ] - Analysis using the Biotin conjugate of NB Comparison between mouse (lung, top) and human (prostate, bottom) staining using anti-cd31 antibody. Data demonstrates that the antibody stains human, but not mouse CD31. Image from verified customer review. Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (JC/70A) [NB ] - Rabbit Heart, CD31 Stained Red. Image from verified customer review.

4 Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB ] - Formalin fixed paraffin embedded human tonsil stained with CD31 antibody. Page 3 of 7 v.20.1 Updated 3/12/2018 Western Blot: CD31/PECAM-1 Antibody (JC/70A) [NB ] - Western blot analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate. Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB ] - Formalin fixed paraffin embedded human tonsil stained with CD31, using ABC method. Flow Cytometry: CD31/PECAM-1 Antibody (JC/70A) [NB ] - A cell surface stain was performed on Jurkat cells with CD31/PECAM-1 (JC/70A) antibody NB AF488 (blue) along with a matched isotype control. Cells were incubated in an antibody dilution of 10 ug/ml for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 488.

5 Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (JC/70A) [NB ] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-cd31 (JC/70A) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Page 4 of 7 v.20.1 Updated 3/12/2018 Flow (Cell Surface): CD31/PECAM-1 Antibody (JC/70A) [NB ] - A cell surface stain was performed on HUVEC cells with CD31/PECAM-1 antibody NB (blue) along with a matched isotype control NBP (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at RT. (Panel A). A negative control (HeLa cells) was also stained to ensure antibody specificity (Panel B).

6 Publications He Y, Mei L, Jin Y, Li X. Overexpression of hepatocyte growth factor mrna induced by gene transfer attenuates neointimal hyperplasia after balloon injury. Hum. Gene Ther Jan 30 [PMID: ] (IHC, Rabbit) Kuang XY, Jiang XF, Chen C et al. Expressions of glia maturation factor-b by tumor cells and endothelia correlate with neovascularization and poor prognosis in human glioma. Oncotarget. Dec :00AM [PMID: ] (IHC, Human) Lin CC, Lin AT, Yang AH, Chen KK. Microvascular Injury in Ketamine-Induced Bladder Dysfunction. PLoS ONE. Aug :00AM [PMID: ] (ICC/IF) Strilic B, Yang L, Albarran-Juarez J et al. Tumour-cell-induced endothelial cell necroptosis via death receptor 6 promotes metastasis. Nature. Aug :00AM [PMID: ] (IHC, Human) Song Y, Zheng J, Yan M et al. The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model PLoS ONE 2015 Jun 27 [PMID: ] (IHC-P, Rabbit) Details: CD31/PECAM-1 antibody (clone JC/70A) was used at 1:50 dilution for IHC-P analysis of irreversible electroporation/ire-ablated segment, a distal segment and a proximal segment of muscle-tendon complexes from Rabbits (Fig 8). Sun Dongchong, Yang Yong, Wei Zhitao et al. Engineering of pre-vascularized urethral patch with muscle flaps and hypoxia-activated hucmscs improves its therapeutic outcome. J Cell Mol Med Jan 25 [PMID: ] (IHC) Kalen M, Heikura T, Karvinen H et al. Gamma-secretase inhibitor treatment promotes VEGF-A-driven blood vessel growth and vascular leakage but disrupts neovascular perfusion. PLoS One Apr 14 [PMID: ] (IHC, Rabbit) Reymond N, Imbert AM, Devilard E et al. DNAM-1 and PVR regulate monocyte migration through endothelial junctions. J Exp Med May 17 [PMID: ] (IHC, FLOW, Human) Longatto Filho A, Oliveira TG, Pinheiro C et al. How useful is the assessment of lymphatic vascular density in oral carcinoma prognosis? World J Surg Oncol Dec 11 [PMID: ] (IHC, Human) Ding MX, Lin XQ, Fu XY Zhang N, Li JC. Expression of vascular endothelial growth factor-c and angiogenesis in esophageal squamous cell carcinoma World J Gastroenterol Jul 28 [PMID: ] (IHC, Human) Zurita AJ, Troncoso P, Cardo-Vila M et al. Combinatorial screenings in patients: the interleukin-11 receptor alpha as a candidate target in the progression of human prostate cancer. Cancer Res Jan 15 [PMID: ] (IHC, Human) Hambrecht R, Adams V, Erbs S. Regular physical activity improves endothelial function in patients with coronary artery disease by increasing phosphorylation of endothelial nitric oxide synthase. Circulation Jul 1 [PMID: ] (IHC, Human) More publications at Page 5 of 7 v.20.1 Updated 3/12/2018

7 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB ml HAF007 NB720-B NBP mg 210-TA-005/CF Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] (Preabsorbed) Recombinant Human CD31/PECAM-1 Protein TNF-alpha [Unconjugated] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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