Product Datasheet. Panendothelial Cell Antigen Antibody (MECA-32) NB Unit Size: 0.5 mg

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1 Product Datasheet Panendothelial Cell Antigen Antibody (MECA-32) NB Unit Size: 0.5 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 14 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 1/30/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Panendothelial Cell Antigen Antibody (MECA-32) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Product Description Host 0.5 mg 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal MECA % Sodium Azide IgG2a Kappa Protein G purified PBS Rat Gene ID Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen Plvap Mouse Mouse. Pan endothelial cells except for CNS & muscle capillaries. Mouse lymph node stromal cells. Page 1 of 4 v.20.1 Updated 1/30/2018 Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunoprecipitation, CyTOF-ready Western Blot 2 ug/ml, Flow Cytometry <= 0.25 ug/10^6 cells in 100 ul, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:10-1:500, CyTOF-ready There have been mixed results for use in Immunohistochemisty on paraffinembedded sections. Immunohistochemistry-Paraffin was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID )

3 Images Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB ] - Western blot analysis of mouse embryonic cell lysate (A) using at a concentration of 2 ug/ml. Page 2 of 4 v.20.1 Updated 1/30/2018 Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB ] - Analysis using the PerCP conjugate of NB Staining of bend.3 cells with MECA-32 AF488.

4 Publications Yanai H, Atsumi N, Tanaka T et al. Intestinal stem cells contribute to the maturation of the neonatal small intestine and colon independently of digestive activity Sci Rep 2017 Aug 31 [PMID: ] (IHC-FR, Mouse) Tarlungeanu DC, Deliu E, Dotter C et al. Impaired Amino Acid Transport at the Blood Brain Barrier Is a Cause of Autism Spectrum Disorder. Cell. Dec :00AM [PMID: ] (IHC, Mouse) Sharmin S, Taguchi A, Kaku Y et al. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation. J. Am. Soc. Nephrol Nov 19 [PMID: ] (IHC-P, Mouse) Ran S, Downes A, Thorpe PE. Increased exposure of anionic phospholipids on the surface of tumor blood vessels. Cancer Res Nov 1 [PMID: ] (IHC, ICC/IF, Mouse) Ma L, Young J, Prabhala H et al. mir-9, a MYC/MYCN-activated microrna, regulates E-cadherin and cancer metastasis. Nat Cell Biol Mar [PMID: ] (IHC, Mouse) Mandriota SJ, Jussila L, Jeltsch M et al. Vascular endothelial growth factor-c-mediated lymphangiogenesis promotes tumour metastasis. EMBO J Feb 15 [PMID: ] (IHC, Mouse) Wogensen L, Huang X, Sarvetnick N. Leukocyte extravasation into the pancreatic tissue in transgenic mice expressing interleukin 10 in the islets of Langerhans. J Exp Med Jul 1 [PMID: ] (IHC, Mouse) Pippin JW, Sparks MA, Glenn ST et al. Cells of Renin Lineage Are Progenitors of Podocytes and Parietal Epithelial Cells in Experimental Glomerular Disease. Am J Pathol 2013 Jun 14 [PMID: ] (IHC, ICC/IF, Mouse) Pan SC, Wu LW, Chen CL et al. Angiogenin expression in burn blister fluid: Implications for its role in burn wound neovascularization Wound Repair Regen 2012 Jul 23 [PMID: ] (IHC, Mouse) Hallmann R et al. Dev. Dyn. 202: [PMID: ] (IHC) Bergese SD et al. Transplantation 57: [PMID: ] (IHC) Leppink DM, Bishop DK, Sedmak DD et al. Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration. Transplantation 48: [PMID: ] More publications at Page 3 of 4 v.20.1 Updated 1/30/2018

5 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF005 Goat anti-rat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] NB7115 Goat anti-rat IgG (H+L) Secondary Antibody [HRP] NBP Rat IgG2a Kappa Isotype Control NB AF ml Panendothelial Cell Antigen Antibody (MECA-32) [Alexa Fluor 647] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: