OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report h been submitted : :49:13 Name of disee (or topic) for which you are a designated OIE Reference Laboratory: Leishmaniosis Address of laboratory: Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi), National Reference Centre for Leishmaniis (C.Re.Na.L.), via Gino Marinuzzi 3, 90129, Palermo ITALY Tel.: Fax: address: fabrizio.vitale@izssicilia.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Salvatore Seminara Name (including Title Position) of OIE Reference Expert: Fabrizio Vitale, DVM Director Molecular Biology Dept. Director of National Reference Center for Leishmaniis (C.Re.Na.L.) Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote disseminate diagnostic methods validated according to OIE Stards 1. Did your laboratory perform diagnostic tests for the specified disee/topic for purposes such disee diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/No) Total number of test performed lt year Indirect diagnostic tests Nationally Internationally Indirect Immunofluorescence Test (IFAT) Direct diagnostic tests Nationally Internationally culture 76 none Real Time RT-PCR No ToR 2: To develop reference material in accordance with OIE requirements, implement promote the application of OIE Stards. To store distribute to national laboratories biological reference products any other reagents used in the diagnosis control of the designated pathogens or disee. 2. Did your laboratory produce or supply imported stard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply stard reference reagents (non OIE-approved) /or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016

3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients Control Positive sera Indirect Immunofluorescent Test Produced 96 ml 6 ml 4 Et Control Negative sera Indirect Immunofluorescent Test Produced 48 ml 6 ml 4 Et Slide Antigens (Kit) Indirect Immunofluorescent Test Produced slides none 1 Et Immunofluorescent conjugate Indirect Immunofluorescent Test Provide 438 ml none 1 Et infantum DNA Real Time RT - PCR Produced 3,7 ml 1,3 ml 2 Et OIE Reference Laboratory Reports Activities,

4 aethiopica DNA Et braziliensis DNA Et donovani DNA Et major DNA Et tropica DNA Et amazoniensis DNA Et 4 OIE Reference Laboratory Reports Activities, 2016

5 infantum strain Culture Produced 3 ml none 1 Et donovani strain Culture Produced 3 ml none 1 Et major strain Culture Produced 3 ml none 1 Et tropica strain Culture Produced 3 ml none 1 Et 4. Did your laboratory produce vaccines? No 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, stardise validate, according to OIE Stards, new procedures for diagnosis control of the designated pathogens or disees 6. Did your laboratory develop new diagnostic methods validated according to OIE Stards for the designated pathogen or disee? OIE Reference Laboratory Reports Activities,

6 7. Did your laboratory develop new vaccines according to OIE Stards for the designated pathogen or disee? No Name of the new test or diagnostic method or vaccine developed Description References (Publication, website, etc.) Analisi Quantitativa mediante PCR Real Time della Lombardo G. et al. (2012). Detection of infantum DNA by realtime PCR in canine oral conjunctival swabs comparison with other diagnostic techniques. Vet Par. 184 culture medium Ctelli G, Galante A, Lo Verde V, Migliazzo A, Reale S, Lupo T, Piazza M, Vitale F, Bruno F. Evaluation of two modified culture media for infantum cultivation versus different culture media. J Paritol Apr;100(2): ToR 4: To provide diagnostic testing facilities,, where appropriate, scientific technical advice on disee control meures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? No 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice w provided GREECE Quality surance IFAT Proficiency test ITALY Quality surance IFAT Proficiency test FRANCE Quality surance Reference Material SPAIN Quality surance Reference Material NEW ZEALAND Quality surance Reference Material GERMANY Quality surance Reference Material GREECE Quality surance PCR-RT Proficiency test ITALY Quality surance PCR-RT Proficiency test 6 OIE Reference Laboratory Reports Activities, 2016

7 ToR 5: To carry out /or coordinate scientific technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country project in Türkiye 2 years monitoring Dog infection in Turkey Dr. Mehmet Erman Or University of Istanbul TURKEY ToR 6: To collect, process, analyse, publish disseminate epizootiological data relevant to the designated pathogens or disees 11. Did your Laboratory collect epizootiological data relevant to international disee control? 12. Did your laboratory disseminate epizootiological data that had been processed analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 2 1. Spada E, Canzi I, Baggiani L, Perego R, Vitale F, Migliazzo A, Proverbio D. Prevalence of infantum co-infections in stray cats in northern Italy. Comp Immunol Microbiol Infect Dis Apr;45:53-8. doi: /j.cimid PubMed PMID: Ctelli G, Bruno F, Vitale F, Roberti M, Colomba C, Giacomini E, Guidotti L, Ccio A, Tolomeo M. In vitro antileishmanial activity of trans-stilbene terphenyl compounds. Exp Paritol Jul;166:1-9. doi: /j.exppara PubMed PMID: b) International conferences: 2 13/02/2016 al 19/02/2016 Abu Dhabi 23/05/2016 al 25/05/2016 Parigi c) National conferences: 6 05/02/2016 al 06/02/2016 convegno Verona 25/02/2016 al 26/02/2016 Congresso - Catania 04/04/2016 Corso di Formazione Padova 16/04/ Corso di Formazione Tolentino (MC) 08/04/ Corso di Formazione Napoli 28/09/2016 al 30/09/2016 Congresso- Lazise del Garda d) Other: OIE Reference Laboratory Reports Activities,

8 (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific technical training for personnel from OIE Member Countries To recommend the prescribed alternative tests or vaccines OIE Stards 14. Did your laboratory provide scientific technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 9 b) Seminars: 0 c) Hs-on training courses: 1 d) Internships (>1 month): 17 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country c Spain 1 a Norway-Oman-Lebanon-Italy 9 d Italy 17 ToR 8: To maintain a system of quality surance, biosafety biosecurity relevant for the pathogen the disee concerned 15. Does your laboratory have a Quality Management System certified according to an International Stard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) certif. accreditamento 2016.pdf 16. Is your laboratory accredited by an international accreditation body? 8 OIE Reference Laboratory Reports Activities, 2016

9 Test for which your laboratory is accredited Accreditation body Screening e titolazione sierologia mediante immunofluorescenza indiretta per le leishmaniosi ACCREDIA Isolamento Leishmanie da materiale biologico di specie recettive Analisi Quantitativa mediante PCR Real time della infantum ACCREDIA ACCREDIA 17. Does your laboratory maintain a biorisk management system for the pathogen the disee concerned? (See Manual of Diagnostic Tests Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) Location No. Participants National Attività dei Centri OIE e Malattie Transfrontaliere IZS Sicilia- OIE- Rotary Club- Regione Siciliana 12/16 Palermo 100 International 2nd Camel Steering Committee Meeting IZS Sicilia-OIE 06/16 Palermo Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Sub-Regional Meeting on Camel Disees 02/16 Abu-Dhabi (UAE) Speaker Sample Collection OIE Reference Laboratory Reports Activities,

10 ToR 10: To establish maintain a network with other OIE Reference Laboratories designated for the same pathogen or disee organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disee? Not applicable (Only OIE Reference Lab. designated for disee) 21. W your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disee by organising or participating in proficiency tests? Not applicable (Only OIE Reference Lab. designated for disee) 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disee on scientific research projects for the diagnosis or control of the pathogen of interest? Not applicable (Only OIE Reference Lab. designated for disee) ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens disees to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disee? Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point OIE Reference Laboratory Reports Activities, 2016

11 Purpose for inter-laboratory test comparisons 1 No. participating laboratories Region(s) of participating OIE Member Countries Harmonising existing test methods(specify. IFAT) 10 Et Evaluating new test methods (specify : Protocol for Real Time RT-PCR) 2 Et Laboratory capability to conduct specific diagnostic tests (specify: typing IFAT Isolation) 1 Et ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Provision of comments on OIE Stards Paris Revision of chapter Leishmaniosis 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,