OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :48:44 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Bovine tuberculosis Address of laboratory: Unité Zoonoses Bactériennes (ACI-ROU-009),Laboratoire de Santé Animale de Maisons-Alfort, ANSES, 14 rue Pierre et Marie Curie, Maisons-Alfort Cedex,FRANCE Tel.: +33 (0) Fax: +33 (0) address: Maria-laura.boschiroli@anses.fr Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Pascal Boireau, Head of the Animal Health Laboratory, Anses, Maisons-Alfort Name (including Title and Position) of OIE Reference Expert: Dr. María Laura Boschiroli-Cara, Research Director, Head of the Tuberculosis National Reference Laboratory Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Serology (wild boar) Tuberculine batch control 6 0 Direct diagnostic tests Nationally Internationally Culture 6 0 Strain identification Molecular diagnosis Spoligotyping MLVA Whole genome sequencing ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016

3 4. Did your laboratory produce vaccines? Bovine tuberculosis - María Laura Boschiroli-Cara - france 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided SPAIN Study of Mycobacterium bovis excretion in naturally infected wild boar by molecular detection 2 month trainee of a doctoral student ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,

4 ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 12 International: Lambert S, Hars J, Réveillaud E, Moyen JL Gares H, Rambaud T, Gueneau E, Faure E, Boschiroli ML, Richomme, C. Host status of wild roe deer in bovine tuberculosis endemic areas, Eur J Wildl Res, 63(1), doi: 1007/s Branger M, Hauer A, Michelet L, Karoui C, Cochard T, De Cruz K, Henault S, Boschiroli ML, Biet F. Draft Genome Sequence of Mycobacterium bovis Strain D Isolated from Wild Boar. Genome Announc. 2016;4(6). pii: e doi: /genomeA Hauer A, Michelet L, De Cruz K, Cochard T, Branger M, Karoui C, Henault S, Biet F, Boschiroli ML. MIRU-VNTR allelic variability depends on Mycobacterium bovis clonal group identity. Infect Genet Evol. 2016;45: doi: /j.meegid Barbier E, Chantemesse B, Rochelet M, Fayolle L, Bollache L, Boschiroli ML, Hartmann A. Rapid dissemination of Mycobacterium bovis from cattle dung to soil by the earthworm Lumbricus terrestris. Vet Microbiol. 2016;186:1-7. doi: /j.vetmic Michelet L, de Cruz K, Phalente Y, Karoui C, Hénault S, Beral M, Boschiroli ML. Mycobacterium microti Infection in Dairy Goats, France. Emerg Infect Dis. 2016;22(3): doi: /eid Barbier E, Boschiroli ML, Gueneau E, Rochelet M, Payne A, de Cruz K, Blieux AL, Fossot C, Hartmann A. First molecular detection of Mycobacterium bovis in environmental samples from a French region with endemic bovine tuberculosis. J Appl Microbiol. 2016;120(5): doi: /jam Chartier C, Albaric O, Cesbron N, Despres J, Hoogveld C, Michelet L, Boschiroli ML. Tuberculoid nodular thelitis in a dairy goat flock. Vet J. 2016;209: doi: /j.tvjl National: Hars J, Lambert L, Moyen JL, Gares H, Viau A, Salvaudon M, Boschiroli ML, Richomme C. Étude épidémiologique sur la tuberculose bovine chez le chevreuil (Capreolus capreolus) en Dordogne. Bull Epid Santé Anim Alim.2016 ; 69 : Masset N, Carole Peroz C, Albaric O, Treilles M, Jacques JP, Boschiroli ML, Chartier C. Découverte atypique d un foyer de tuberculose à Mycobacterium bovis dans un élevage mixte caprin-bovin. Bulletin des GTV.2016 ; 82 : OIE Reference Laboratory Reports Activities, 2016

5 Masset N, Carole Peroz C, Albaric O, Treilles M, Jacques JP, Boschiroli ML, Chartier C. Enquête et mesures sanitaires à la suite d un foyer de tuberculose dans un troupeau mixte caprin-bovin. Bulletin des GTV.2016 ; 83 : Chevalier F, Hars J, Courcoul A, Hansen E, Boschiroli ML, Richomme C. Investigations lors d un cas de tuberculose bovine découvert chez un sanglier dans une zone indemne en élevage (Loir-et-Cher, 2015) : retour d expérience. Epidemiol et Santé anim ; Michelet L, de Cruz K, Phalente Y, Bulach T, Karoui C, Hénault S, Boschiroli ML. Le diagnostic de la tuberculose bovine en France : la biologie moléculaire à la rescousse de l histopathologie. Epidemiol et Santé anim ; 70 : b) International conferences: 6 Michelet L, Hauer A., Cochard T., Branger M., Biet F., Boschiroli ML. From whole genome sequencing data toward a simple genotyping tool : application to the animal pathogen Mycobacterium bovis. 26th ECCMID, 09-12/04/2016, Amsterdam, The Netherlands. Hartmann A, Barbier E, Boschiroli ML. Environmental niches for Mycobacterium bovis in Côte d Or (France). MedVetNet Organisation TB workshop TB in European wildlife: review of surveillance and control measures /05/2015, APHA, New Addleston, United Kingdom. Monchatre E, Barrat J, Boschiroli ML, Richomme C, Lesellier S. Mel-Vac studies. MedVetNet Organisation TB workshop TB in European wildlife: review of surveillance and control measures /05/2015, APHA, New Addleston, United Kingdom. Yaici M, Biet F, Etienne G, Boschiroli ML, Moyen JL. Potential of Mass Spectrometry for routine identification of Mycobacterium avium subsp. paratuberculosis. 13th International Colloquium on Paratuberculosis, 20-24/06/2016, Nantes, France. Boschiroli ML. First line Molecular diagnosis of bovine tuberculosis in France and its articulation with the new reinforced national control program. VIII SLAMTB Meeting, XXIII Latin American Congress of Microbiology, XIV Argentine Congress of Microbiology. Convention Center Metropolitano, 26-30/09/2016, Rosario, Argentina. ML. Boschiroli.TB in Wild and Domestic Animals. EAZA/EAZWV First Tuberculosis Workshop, 8-9/12/2016, Parc Zoologique de Vincennes, Paris, France c) National conferences: 4 Boschiroli ML. La tuberculose dans la faune sauvage : quelle est la réelle situation en France? Symposium Ensemble pour la Biodiversité. 04/02/2016., Ecole vétérinaire de Toulouse, Toulouse, France. Michelet L, Phalente Y, Karoui C, de Cruz K, Hénault S, Boschiroli ML. Amélioration dans le diagnostic direct de la tuberculose bovine : la biologie moléculaire à la rescousse de l histopathologie. Journées de l AEEMA, 24-25/03/2016, Ecole Vétérinaire de Maisons Alfort, Maisons-Alfort, France. Chevalier F., Hars J., Courcoul A., Hansen E., Boschiroli ML, Richomme C. Investigation lors d un cas de tuberculose découvert chez un sanglier dans une zone indemne de tuberculose bovine (Loir-et-Cher, 2015). Journées de l AEEMA, 24-25/03/2016, Ecole Vétérinaire de Maisons Alfort, Maisons-Alfort, France. Michelet L., Hauer A., Cochard T., Branger M., Biet F., Boschiroli ML. Des données de séquençage de génome complet au développement d un outil de typage. 6èmes journées du Pôle Société Santé consacrées «Agents infectieux et vaccins chez l homme et l animal» /03/2016, Ecole Vétérinaire de Maisons Alfort, Maisons- Alfort, France. d) Other: (Provide website address or link to appropriate information) 2 data on French animal tuberulosis: data on French free-ranging wildlife tuberculosis: OIE Reference Laboratory Reports Activities,

6 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Culture Molecular diagnosis Accreditation body Cofrac Cofrac 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? 6 OIE Reference Laboratory Reports Activities, 2016

7 ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Direct extraction and microbiological culture (organised by the bovine tuberculosis EURL) participant unknown France & United Kingdom 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories MelVac Control of badger disease by vaccination in order to avoid spillover to cattle Bovine Tuberculosis OIE reference laboratory, APHA, UK ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? OIE Reference Laboratory Reports Activities,

8 te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries IFNg test 10 Africa Americas Asia and Pacific Europe Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: 8 OIE Reference Laboratory Reports Activities, 2016