OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2014 This report has been submitted : :03:47 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Leishmaniosis Address of laboratory: Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi), OIE Reference Laboratory National Reference Centre for Leishmaniasis (C.Re.Na.L.), via Gino Marinuzzi 3, 90129, Palermo ITALY Tel.: Fax: address: fabrizio.vitale@izssicilia.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Rossella Colomba Lelli, DVM Name (including Title and Position) of OIE Reference Expert: Dr. Fabrizio Vitale, DVM, Director of Molecular Biology Dept. Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic s for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency ing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of performed last year Indirect diagnostic s Nationally Internationally Indirect Immunofluorescence Test s none Direct diagnostic s Nationally Internationally Leishmania culture 199 none Real Time RT-PCR 1900 s none ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2014

3 Type of reagent available Related diagnostic Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Control Positive sera Indirect Produced 77 ml none 1 Americ as Asia and Pacific Middle East Control Negative Sera Indirect Produced 77 ml none 1 Americ as Asia and Pacific Middle East Slides antigens Indirect Produced slides none 1 Americ as Asia and Pacific Middle East conjugate Indirect Provide 61 ml none 1 Americ as Asia and Pacific Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? OIE Reference Laboratory Reports Activities,

4 ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Name of the new or diagnostic method or vaccine developed Description and References (Publication, website, etc.) Analisi Quantitativa mediante PCR Real Time della Leishmania Lombardo G. et al. (2012). Detection of Leishmania infantum DNA by real-time PCR in canine oral and conjunctival swabs and comparison with other diagnostic techniques. Vet Par. 184 ToR 4: To provide diagnostic ing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic ing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses FRANCE may SPAIN september 2 kits 2 kits TURKEY june none Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided AFGHANISTAN Monitoring the epidemiological data on canine Leishmaniasis remote assistance 4 OIE Reference Laboratory Reports Activities, 2014

5 ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 1) Migliazzo A, Vitale F, Calderone S, Puleio R, Binanti D, Abramo F. Feline leishmaniosis: a case with a high parasitic burden. Vet Dermatol Oct 18. doi: /vde [Epub ahead of print] PubMed PMID: ) Giammanco GM, Di Bartolo I, Purpari G, Costantino C, Rotolo V, Spoto V, Geraci G, Bosco G, Petralia A, Guercio A, Macaluso G, Calamusa G, De Grazia S, Ruggeri FM, Vitale F, Maida CM, Mammina C. Investigation and control of a rovirus outbreak of probable waterborne transmission through a municipal groundwater system. J Water Health Sep;12(3): doi: /wh PubMed PMID: ) Castelli G, Galante A, Lo Verde V, Migliazzo A, Reale S, Lupo T, Piazza M, Vitale F, Bruno F. Evaluation of two modified culture media for Leishmania infantum cultivation versus different culture media. J Parasitol Apr;100(2): doi: / Epub 2013 Dec 18. PubMed PMID: ) Amodio E., Calamusa G., Sidoti E., Migliazzo A., Vitale F., Costantino C., Vitale F., Seroprevalence of and risk factors for Leishmania seropositivity in a sample population of Western Sicily (Italy) Epidemiology Biostatistics and Public Health , Volume 11, Number 2. NBN: urn:nbn:it:prex DOI: /9024 Published as Online First on June 3, ) Ceccarelli M, Galluzzi L, Migliazzo A, Magnani M. Detection and characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA. PLoS One Feb 13;9(2):e doi: /journal.pone ecollection PubMed PMID: ; PubMedCentral PMCID: PMC b) International conferences: 3 8thInternational Symposium on Phlebotomine Sandflies (ISOPS VIII), September 2014, Iguazù, Argentina, th Global Microbial Identifier Meeting, September 2014, York, United Kingdom 3 International Conferences of OIE Reference Laboratories and Collaborating Centres, October 2014, Seoul OIE Reference Laboratory Reports Activities,

6 (Rep. of Korea). c) National conferences: 10 1) Leishmaniosi canina, cosa è cambiato negli ultimi tre anni?, Palermo 30 marzo ) "Leishmaniosi: un problema sottostimato e attuale di sanità pubblica", Agrigento 05 aprile ) "Training course on diagnosis of equine disease", Palermo aprile ) Corso sulle Malattie Emergenti Brescia 07 maggio ) Corso sulle Malattie Emergenti Milano 08 maggio ) "Le attività diagnostiche nella pratica di laboratorio delle malattie infettive",palermo 05 settembre ) Leishmaniosi, a che punto è la ricerca e la relativa terapia tra medicina veterinaria e medicina umana, Marsala 19 settembre ) Giornata di formazione sulla West Nile Disease, Catania 28 ottobre ) "Gestione e Benessere degli animali ospitati nei canili", Palermo Dicembre ) "IL RUOLO DEI CENTRI DI REFERENZA OIE NEL CONTROLLO DELLE MALATTIE TRASMESSE DA ZECCHE ED ALTRI VETTORI", Palermo Dicembre 2014 d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative s or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 6 b) Seminars: 0 c) Hands-on training courses: 0 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Germany 6 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity 6 OIE Reference Laboratory Reports Activities, 2014

7 relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) Cert. accred..pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Accreditation body Screening e titolazione sierologia mediante immunofluorescenza indiretta per le leishmaniosi ACCREDIA Isolamento Leishmanie da materiale biologico di specie recettive Analisi quantitativa mediante PCR real time della Leishmania infantum ACCREDIA ACCREDIA 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) Location. Participants International Training Course on diagnosis of Equine Diseases OIE April 2014 Palermo Did your laboratory participate in scientific meetings on behalf of the OIE? OIE Reference Laboratory Reports Activities,

8 Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented 10th OIE Seminar on the New Approaches to Diagnosis: Opportunities and Challenges Symposium 5 8 June 2013 Berlin (Germany) none none Third Global Conference of OIE Reference Centres October 2014 Incheon (Korea [Rep. of]) none none ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency ing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? t applicable (Only OIE Reference Lab. designated for disease) 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency s? t applicable (Only OIE Reference Lab. designated for disease) 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? t applicable (Only OIE Reference Lab. designated for disease) ToR 11: To organise inter-laboratory proficiency ing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency s with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory comparisons in: Laboratory Proficiency Testing at: see point OIE Reference Laboratory Reports Activities, 2014

9 Purpose for inter-laboratory comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Harmonising existing methods (specify:ifat) 9 Americas Asia and Pacific Middle East Evaluating new methods (specify : Protocol for Real Time RT-PCR) 2 Americas Asia and Pacific Middle East Laboratory capability to conduct specific diagnostic s (specify: typing IFAT and Leishmania Isolation) 10 Americas Asia and Pacific Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Provision of comments on OIE Standards Paris Revision of chapter Leishmaniosis Additional comments regarding your report: The laboratory has been designed as OIE Reference Laboratory on June 9th This report relates from June 9th to December 31st OIE Reference Laboratory Reports Activities,