Automated Handheld Cell Counting Scepter Technology. Next Generation. Scepter 2.0 is here! PINPOINT ACCURACY

Transcription

1 Automated Handheld Cell Counting Scepter Technology Next Generation Scepter 2.0 is here! PINPOINT ACCURACY

2 THE NEW, MORE ACCURATE WAY TO COUNT CELLS The first and only device to bring consistency in cell counting right to the culture hood is here: the Scepter handheld, automated cell counter. While other automated counters consume bench space and rely on object recognition software, manual focusing, and clumsy loading chambers, the Scepter cell counter provides true automation without the error that accompanies vision-based systems. With its microfabricated, precision-engineered sensor, the Scepter cell counter does all the work and delivers accurate and reliable cell counts in less than 20 seconds. 2

3 The Gold Standard Counter: Smaller than Ever. The Scepter cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample. Particles are detected by Ohm s Law V=IR (V=voltage, I=current, and R=resistance) + - Precise volumes are drawn into the Scepter sensor As cells flow through the aperture in the sensor, resistance increases. This increase in resistance causes a subsequent increase in voltage. Voltage changes are recorded as spikes with each passing cell. Spikes of the same size are bucketed into a histogram and counted. This histogram gives you quantitative data on cell morphology that can be used to examine the quality and health of your cell culture. Accurate Counts Every Time You work too hard to lose data to erroneous cell counts. Be confident that your counts are right the first time. Scepter counting gives you the accuracy of the Coulter Counter at a price you can afford. 80% 70% 60% Percent CV 50% 40% 30% 20% 10% 0% Scepter cell counter Vision-based counter Hemocytometer = Average %CV = Coulter Counter %CV Figure 1. The average percent coefficient of variation (CV) for each counting method shown was calculated using cell concentration measurements at 50,000 cells/ml samples of 19 different cell lines. The Scepter cell counter is more precise than vision-based counting and hemocytometry, and approaches the precision of the Coulter Counter standard (red bars). Error bars represent standard deviation. 2 3

4 Gain insight through powerful counting techniques See your cultures with unprecedented detail Easy-to-read histograms and numerical statistics Data manipulation and storage on Scepter device or with software Track cell health as you monitor histograms from one preparation to the next 4

5 ACCURACY THROUGH SENSOR TECHNOLOGY Precisely counts consistent sample volumes Accurately counts every cell in your sample Cell discrimination at sub-micron and sub-picoliter resolution INTRICACY SIMPLIFIED BY TRULY AUTOMATED DATA ACQUISITION Pipette once for data in seconds Use on-site right at the hood Intuitive and easy to use with on screen instructions 5

6 Scepter Technology: More Intuitive, More Informative, and More Precise Cell Counter Format Counting Methods Comments Hemocytometer Slide and microscope Manual, vision-based User bias and human error create irreproducible results Brand I Benchtop Automated vision-based system Object recognition software-dependent, requires focusing and calibration, and can produce unreliable results Scepter Cell Counter Handheld Impedance-based cell detection Counts every cell, every time in very precise volumes to ensure accurate results There is no need to subjectively determine cell counts using vision-based counting methods. The Scepter cell counter detects every cell, and displays the population as a histogram of cell size distributions. From the histogram, count all the cells or use the gating function to count a chosen subpopulation. By monitoring changes in your histogram, you can gain insight into the health and quality of your cell culture from one experiment to the next. 1 Axis= Diameter or Volume 2 Concentration 3 Mean Cell Volume 4 Mean Cell Diameter 300 Count Test volume pl 3.124e5 / ml Jun 11 20:45: Count Test volume pl 3.124e5 / ml Jun 11 20:45:13 Measured Cell Concentration (Cells/mL) 7.0E E E E E E E E Theoretical Cell Concentration (Cells/mL) Coulter counter Flow cytometer Hemocytometer Automated vision-based counter Scepter cytometer Theoretical cell concentration n (cells/ml) Figure 2. COS7 cell counts using various systems. Data shown are average of four replicates. Error bars represent standard deviation. COS7 cells represent one out of 19 total cell lines tested. Cells were harvested, diluted in PBS, and counted using a Coulter Counter to determine the theoretical starting concentrations. The original cell concentration was divided by the fold dilution at each serial dilution step to determine theoretical concentrations (x axis). 6

7 Exhaustively tested for your peace of mind We tested over 20 cell lines including adherent, suspension, differentiated and progenitor cells to validate the Scepter cell counter s precision and operating range. Scepter Cell Count (Cells/mL) 7.0E E E E E E E+05 K562 r 2 = U266 r 2 = HeLa r 2 =0.999 Mesenchymal SC r 2 = SF9 r 2 =0.998 Theoretical cell concentration (cells/ml) 0.0E Theoretical Cell Concentration (Cells/mL) Figure 3. Scepter counting performs with high linearity (R2 > 0.993) across multiple, diverse cell lines, over a wide operating range. Shown here are cell concentration data for five representative lines out of 19 total cell lines tested. Tested Cell Lines Cell Line Name Measured Average Size (µm) Adherent / Suspension Origin Source HEK adherent Human kidney Invitrogen Cat. No. R CHO-K1 15 adherent Chinese hamster ovary ATCC Cat. No. CCl-61 HeLa 13 adherent Human cervical cancer Millipore Cat. No NIH3T3 15 adherent Mouse fibroblasts ATCC Cat. No. CRL-1658, MCF adherent Human breast adenocarcinoma ATCC Cat. No. HTB-22 Cos-7 15 adherent Monkey kidney cells Genlantis Cat. No. C Jurkat 10 suspension T lymphocytes ATCC Cat. No. TIB-152 SF9 13 mix adh / susp Insect ovary (baculovirus expression) ATCC Cat. No. CRL 1711 PC loosely adherent Rat adrenal gland ATCC Cat. No HepG2 15 adherent Hepatocytes ATCC Cat. No. HB8065 K suspension Human bone marrow ATCC Cat. No. CCL243 U suspension B lymphocytes ATCC Cat. No. TIB-196 RNSC adherent Rat neuronal stem cells Millipore Cat. No. scr021 Epithelia adherent Epithelia CELLnTEC Cat. No. HPEK p.05 HUVEC adherent Human endothelial Millipore/ISCO Cat. No. SCCE001 Mouse ESC 13 adherent Mouse embryonic stem cells Mesenchymal SC adherent Human bone marrow mesenchymal SC Millipore Cat. No. SCR012 Millipore Cat. No. SCR108 3T3-L adherent Human pre-adipocytes ATCC Cat. No. CL-173 hybridoma suspension Hybridoma (Irs1 ps ) Children's Hospital Boston Cat. No Table 1. The cell lines tested represent adherent cells, suspension cells, differentiated, pluripotent, normal and transformed cells, and cover the range of cell types used in most cell research laboratories. 6 7

8 ORDERING INFORMATION Description Quantity Catalog No. Scepter 2.0 Handheld Automated Cell Counter with 40 µm Scepter Sensors (50 Pack) 1 PHCC20040 with 60 µm Scepter Sensors (50 Pack) 1 PHCC20060 Includes: Scepter Cell Counter 1 Downloadable Scepter Software 1 O-Rings 2 Scepter Test Beads 1 PHCCBEADS Scepter USB Cable 1 PHCCCABLE Scepter Sensors, 60 µm 50 PHCC PHCC60500 Scepter Sensors, 40 µm 50 PHCC PHCC40500 Universal Power Adapter 1 PHCCP0WER Scepter O-Ring Kit, includes 2 O-rings and 1 filter cover 1 PHCC0CLIP Are you an existing Scepter user interested in upgrading your device to Scepter 2.0? It s easy. Visit to upgrade your Scepter today! For technical assistance, contact Millipore: MILLIPORE ( ) tech_service@millipore.com Millipore, Advancing Life Science Together, and EmbryoMax are registered trademarks of Millipore Corporation. Scepter and the M mark are trademarks of Millipore Corporation. Excel is a registered trademark of Microsoft Corporation. Vi-CELL and Coulter Counter are registered trademarks of Beckman Coulter, Inc. Z2 is a trademark of Beckman Coulter, Inc. Countess is a trademark of Life Technologies, Inc. Fisher Scientific BN Printed in U.S.A. 04/2011 BS-GEN Millipore Corporation, Billerica, MA U.S.A. All rights reserved.