Handheld Automated Cell Counter

Transcription

1 Meet Scepter Technology Handheld Automated Cell er

2 Streamline Your Cell Culture Workflow with Scepter Technology No more squiing through the microscope, straining your eyes and fingers. With its microfabricated, precision-engineered tip, the Scepter cytometer does all the work. Use the Scepter cytometer to: cells accurately for cell-based assays Split cells, seed and passage cells several samples quickly Quaify cell proliferation rates Monitor cell diameter and volume Determine effects of varying culture conditions on cell population distributions SCEPTER CELL COUNTER AND ACCESSORIES Description Qty/Pk Catalogue No. Scepter Handheld Automated Cell er 1 PHCC Includes: Scepter Cell er 1 Scepter Software CD 1 O-Rings 2 Scepter Test Beads 1 PHCCBEADS Scepter USB Cable 1 PHCCCABLE Scepter Tips, 6 µm 5 PHCC65 5 PHCC65 Universal Power Adapter 1 PHCCPOWER Scepter O-Ring Kit, coains three O-rings and one filter cover 1 kit PHCCCLIP Related Products Description Qty/Pk Catalogue No. EmbryoMax 1x Dulbecco s Phosphate Buffered Saline w/o Ca 2+ and Mg 2+ 1 L BSS-16-A

3 Experience Scepter Technology Liberate Yourself From the Microscope Easy to use Looks and works like a pipette On-screen instructions USB port for downloading data and charging Stores 72 histograms A New Window on Your Cell Cultures Histogram data on cell populations, distributed by cell size or cell volume Cell conceration Mean cell volume and size Can apply custom gating Gain insight io cell health volume pl Test e5 / ml 2.68pL 17.23µm Technology Packed io a Pipette Tip Iegrated cell sensing electrodes Precision molded sampling chamber Precision manufactured electronic sensing zone Cell size discrimination at sub-micron resolution Cell volume discrimination at sub-picoliter resolution

4 As easy as pipetting Prepare the sample: Start with a single-cell suspension, diluted to a total volume of 1 µl (recommended) in phosphate buffered saline (such as EmbryoMax 1x DPBS) to 1,-5, cells/ml (operating range) in a 1.5 ml microcerifuge tube. Perform cell co: Turn on the Scepter cytometer by pressing the toggle on the back of the instrume and wait for on-screen instructions to appear. When prompted, attach a tip to the end of the Scepter unit with the electrode sensing panel facing toward the fro of the instrume, and you ll see detailed instructions for each step of the coing process. Pipette once to draw sample io the tip. 5 µl of your cell suspension is drawn io the microfabricated, precision-engineered channel embedded in the tip. The cell sensing zone detects each cell drawn io the tip and thus cell conceration is calculated. The sensing zone also measures cell sizes and cell volumes with sub-micron and sub-picoliter resolution, enabling the Scepter cytometer to display a histogram distribution of cell size or cell volume. 16n 16n 17n 17n 17n 18n 18n 18n 19n 19 BEEP wn Hold the down Hold thedown Submerge the Submerge Submerge BEEP er plunger to to plunger the tip to the tip the WAITtip in begin begin attached 6µm tip attached 6µm tip Release attached plunger Release plunger Release plunger Ready... Ready... 6µm tip attached 6µm tip attached 6µm tip attached 1% WAIT 1% 1% Keep tip Keep tip Keep tip submerged submerged submerged WAIT... Click to cancel Click to cancel Click to cancel loading sample loading... sample loading... sample... Loaded Loaded Loaded remove tip from remove sampletip from remove sample tip f sample loaded. sample loaded. sample load n 21n wn Hold the 2n down the 2n Submerge Submerge Submerge er plunger to to the tip the tip the tip gin begin 9 submerged submerged submerged Complete. Complete. Loaded Complete. Loaded Loaded Please remove Please removeplease remove attached 6µm tip attached Release plunger Release plunger Release plunger Click to cancel Click tip to and cancel discard. Click tip to and cancel discard. tip remove and discard. tip from remove sampletip from remove sample tip Ready... 6µm tip attached 6µm WAIT... tip attached 6µm tip attached loading WAIT... REMOVE sample loading... sample loading... sample... sample loaded. sample loaded. sample load TIP dia. µm dia. µm dia. µm dia. µm dia. µm dia. µm dia. µm dia. µm dia. µm dia. µm lease saving wait... Please saving wait... Please saving wait... Please saving wait... Please saving wait... Please wait... Please wait... Please wait... Test 3.124e5 Test/ ml 3.124e5 alculating... calculating... saving... saving... saving... Jun 11 2:45:13 Jun 11 2:45:13 Jun 11 2:45:13 3 Jun 11 2:45:13 3 Jun n WAIT... 1% Keep tip 21n Keep tip 1% 22n Keep tip 1% 22n BEEPBEEP WAIT WAIT 22n 23n Complete. Complete. Complete. Please remove Please removeplease remove

5 ge down the nger to begin % Keep Iuitive Submerge tip data analysis submerged the tip ger Click to cancel remove tip from sample tip attached Set your gates: Release plunger Click to cancel ched loading sample... sample loaded... 6μm tip attached loading sample... Set your gates (upper and lower limits of the histogram) manually on the histogram 1% Keep tip submerged Loaded profile or use the automatic gating capabilities matched to the previous co s gating. 23n 23n 49n 49n 24n 24n 3 Loaded.11 v Please calcula wai remove tip from calculating sample loaded. 6 Test dia. µmdia. µm dia. µm 3.124e5 Test 3.124e5 Test / ml 3.124e5 / ml / ml Jun 3 11 Jun 2:45: :45:13 Jun 11 2:45:13 6 Test dia. µmdia. µm dia. µm 3.124e5 Test 3.124e5 Test / ml 3.124e5 / ml / ml Jun 3 11 Jun 2:45: :45:13 Jun 11 2:45: dia. µmdia. µm dia. µm Test 3.124e5 Test 3.124e5 Test / ml 3.124e5 / ml / ml pL pL 16.19µm pL 16.19µm 16.19µm Store up to 72 histograms on the Scepter unit, or upload to a personal computer with distribution data as shown below. Easily share your data or conduct in-depth analysis by volume volume pl pl exporting uploaded files to Microsoft volume Excel volume pl spreadsheet pl software. volume volume pl pl Test 3.124e5 Test 3.124e5 / ml / ml Test 3.124e5 Test 3.124e5 / ml / ml Test 3.124e5 Test 3.124e5 / ml / ml 3 Jun 3 11 Jun 2:45: :45:13 3 Jun 3 11 Jun 2:45: :45: pL pL 16.19µm 16.19µm 49 Store, upload, and manipulate data: included software. Scepter Application Software displays uploaded volume and size

6 Why use Scepter coing over other cell coing systems? Because Scepter coing is linear across diverse cell types. We tested over 2 cell lines including adhere, suspension, differeiated and progenitor cells to validate the Scepter cytometer s precision and operating range. Scepter Cell (Cells/mL) 7.E+5 6.E+5 5.E+5 4.E+5 3.E+5 2.E+5 1.E+5 K562 r 2 =.9982 U266 r 2 =.9996 HeLa r 2 =.999 Mesenchymal SC r 2 =.9938 SF9 r 2 =.998 Theoretical cell conceration (cells/ml).e Theoretical Cell Conceration (Cells/mL) Cells of various types (adhere cancer cells (HeLa), suspension differeiated cells (U266, K562), suspension insect cells (SF9), and mesenchymal stem cells) were harvested and coed with a Scepter cytometer. s are averages of 4 replicates, and error bars represe standard deviation. Because Scepter coing is precise and accurate. Scepter coing shows comparable accuracy and linearity to Coulter coing, without the strain of manual coing. Measured Cell Conceration (Cells/mL) 7.E+5 6.E+5 5.E+5 4.E+5 3.E+5 2.E+5 1.E+5.E Theoretical Cell Conceration (Cells/mL) Coulter coer Flow cytometer Hemocytometer Automated vision-based coer Scepter cytometer Theoretical cell conceration (cells/ml) Multiple cell types (COS7 cells shown here) were harvested and coed using the methods shown. s are averages of 4 replicates, and error bars represe standard deviation.

7 Because Scepter coing is fast. Scepter coing is ten times faster than hemocytometry, and faster than other automated coers, too. With Scepter technology, you ll save time and eliminate tedium Time (seconds) Scepter cytometer Flow cytometer Coulter coer Automated vision-based coer Hemocytometer Cells (SF9, MCF7, and HEK293) were coed using the methods shown, and average time required to measure cell conceration was recorded. (Hemocytometry was performed only once per sample.) New! Millicell HY Multilayer Culture Flasks Your big experime is just a few passages away. Consistely high cell yields, uniform health across all layers Same media requiremes (volume per unit area) as traditional T-flasks Familiar form enables easy transition from single-layer culture flasks Easy media removal from flask with ergonomic cap location Uniform culture conditions without media leaking or spillover between layers Fits under microscopes for monitoring cell health and confluency Stackable design saves incubator space Barcoding available for automated cell culture systems Description No. of Layers Total Surface Area (cm 2 ) Qty/Pk Catalogue No. Millicell HY Flask* PFHYS616 STEM CELL TESTED PFHYS18 * Available with barcodes please coact technical service for more information. Stem cell testing was conducted by using the device in question for culturing mesc for five passages, then verifying by flow cytometry that viability and pluripotency are unchanged.

8 TO PLACE AN ORDER OR RECEIVE TECHNICAL ASSISTANCE In the U.S. and Canada, call toll-free 1 8-Millipore ( ) In Europe, please call Customer Service: France: Spain: Option 1 Germany: Italy: United Kingdom: For other cories across Europe and the world, please visit For Technical Service, please visit Millipore, Advancing Life Science Together, Millicell, and EmbryoMax are registered trademarks of Millipore Corporation. The M mark and Scepter are trademarks of Millipore Corporation. Microsoft and Excel are registered trademarks of Microsoft Corporation. Lit. No. PB123EN Pried in U.S.A. 1/1 BS-GEN Millipore Corporation, Billerica, MA 1821 U.S.A. All rights reserved.