Genetix Biotech. Vacuum connects automatically. Once fluid is added to the top once to the back of the Autofil TM. when filter is placed on the

Transcription

1 Autofil Bottle Top Filters Autofil is the most innovative bottle top vacuum operated laboratory filtration system in the world. The filters are used for the sterile preparation of buffers, tissue culture media, microbiological media, and other biological fluids. Fluid andling System Sterile Vacuum Filtration of Aqueous Fluids INDEX Filtration System Autofil 281 Cellulose Nitrate Membrane (ESTERS) Filters 283 Gridded Cellulose Nitrate Membrane Disc Filter 284 Cellulose Acetate Membrane Filter 284 Nylon-66 Membrane Filter 285 Nylon-66 Membrane Disc Filter 285 Nitrocellulose Membranes 286 Nylon 6,6 Blotting Membranes 287 Cellulose Acetate Syringe Filters 288 PES (Poly Ethersulfone) Syringe Filters 288 PVDF (Poly Vinylidene Fluoride) Syringe Filters 289 Nylon Syringe Filters Filtration System Autofil Benefits of using the Autofil TM filtration System 3 Vacuum hose is connected just one time 3 Pedestal offers fast connection and hands-free operation 3 Forward facing graduation marks on tubes and bottles 3 Vacuum on and off switch conveniently located 3 Autofil TM design including an ergonomic bottle shape. molded finger grips and patented bottle cap 3 Conveniently sold presterilized 3 Autofil TM is available as a complete system or as a bottles-top filter A B Protein Binding increased throughput (90mm Membrane) A Centrifuge tubes available in 15ml and 50ml B Bottles available in 250ml, 500ml and 1000ml sizes 0.22µm and 0.45µm PES membrane pore-size options available Available in 0.22µm or 0.45µm asymmetric membrane filter Easy initial setup of Autofil TM filtration System 1 Connect a vacuum tube just 2 Vacuum connects automatically 3 Once fluid is added to the top once to the back of the Autofil TM when filter is placed on the of the Autofil TM system, turn the pedestal. Autofil TM pedestal. vacuum switch on.

2 282 Autofil Filtration System Complete Assembly GX CA Autofil, Complete Assembly, PES, 15ml,.22µm, 24/Cs 12,771 GX CA Autofil, Complete Assembly, PES, 15ml,.45µm, 24/Cs 12,771 GX CA Autofil, Complete Assembly, PES, 50ml,.22µm, 24/Cs 12,771 GX CA Autofil, Complete Assembly, PES, 50ml,.45µm, 24/Cs 12,771 GX CA Autofil, Complete Assembly, PES, 250ml, 66mm,.22µm, 12/Cs 7,822 GX CA Autofil, Complete Assembly, PES, 250ml, 66mm,.45µm, 12/Cs 7,822 GX CA Autofil, Complete Assembly, PES, 500ml, 90mm,.22µm, 12/Cs 10,633 GX CA Autofil, Complete Assembly, PES, 500ml, 90mm,.45µm, 12/Cs 9,707 GX CA Autofil, Complete Assembly, PES, 1000ml, 90mm,.22µm, 12/Cs 12,182 GX CA Autofil, Complete Assembly, PES, 1000ml, 90mm,.45µm, 12/Cs 12,182 Bottle-Top Only GX BT Autofil, Bottle-Top, PES, 250ml, 66mm,.22µm, 24/Cs 11,217 GX BT Autofil, Bottle-Top, PES, 250ml, 66mm,.45µm, 24/Cs 11,217 GX BT Autofil, Bottle-Top, PES, 500ml, 90mm,.22µm, 24/Cs 12,817 Selection Guide Pore Size Best For 0.1μm 0.2μm 0.45μm 0.8μm Cell Culture (Sterilization & Mycoplasma removal) Cell Culture (Sterilization) Buffers (clarification, particle removal) Prefilteration and Clarification Membrane Attributes Best For PES Lowest protein binding, lowest Cell Culture media and serum extractables, fastest flow SFCA Lower extractables than standard Cell Culture media and serum cellulose acetate CN igher extractables Buffers Nylon igher protein binding, Alcoholic solutions alcohol resistance GX BT Autofil, Bottle-Top, PES, 500ml, 90mm,.45µm, 24/Cs 12,817 GX BT Autofil, Bottle-Top, PES, 1000ml, 90mm,.22µm, 24/Cs 20,028 GX BT Autofil, Bottle-Top, PES, 1000ml, 90mm,.45µm, 24/Cs 20,028 Pedestal Stand GX-81PDS Pedestal Stand 58,710 Bottles Only GX PS 250ml Bottle Only ( 24/Cs) 6,080 GX PS 500ml Bottle Only ( 24/Cs) 8,718 GX PS 1000ml Bottle Only ( 24/Cs) 10,697 GX PC 250ml Bottle Only ( 24/Cs) 7,776 GX PC 500ml Bottle Only ( 24/Cs) 11,735 GX PC 1000ml Bottle Only ( 24/Cs) 15,880 GX PETG 250ml Bottle Only (24/Cs) 7,304 GX PETG 500ml Bottle Only (24/Cs) 10,697 GX PETG 1000ml Bottle Only (24/Cs) 13,902 Selection Chart For Blotting Membranes This selection chart highlights the suitability of various blotting membranes for different applications based on their properties. Membrane Type SCN Pure SNN Pure Cellulose Nitrate Nylon-66 Biomolecules Nucleic Acids R R Proteins R R Transfer Method Dot Blot R R Colony or Plaque Lift R R Electrotransfer R* R Capillary Blot R R Vaccum Blot R R Alkaline Transfer NR R Molecule Fixation Baking R R Drying R R UV Crosslinking P R Alkali Fixation NR R Molecule Removal NR NR Detection Method Colorimetric R R Radiolabelled R R Luminescent R P Fluorescent R P Staining R P Reprobing Once NR R Multiple NR R R=ighlyRecommended, R=Recommended, R*=RecommendedforProteinsonly, P=Possible, NR = Not Recommended

3 Membrane Filters Cellulose Nitrate Membrane Disc Filters are hydrophilic, non-media, migrating, biologically inert, plain white absolute membrane filters available in a wide range of pore sizes. Features and Benefits 3 Absolute retention 3 igh flow rates 3 Narrow pore size distribution 3 Low extractable levels 3 Sterilisation: Autoclavable at 121 C in dry conditions 3 Maximum Operating Temperature: 80 C continuous 3 Maximum Operating Pressure: 3 Kg/cm2 3 Biosafety: Passed the Biological tests for Class VI plastics as described in USP 3 Oxidizable Matter: Passed as per USP Cellulose Nitrate Membrane (ESTERS) Filters Cellulose nitrate membranes have a very uniform pore structure and are available in a wide range of pore sizes. 283 Cellulose Nitrate Membrane Filters 0.2 um for the sterile filtration of aqueous buffer water 0.45um pore size for particle collection, filtering beverages etc. The larger pore sizes (5um, 3um) find use for chemo taxis and cell and particle retentions stidies. The high non-specific adsorption of the cellulose nitrate membrane is advantageous for diagnostic kits. The adsorption decrease with increasing pore sizes. ONO 2 C 2 ONO 2 O O ONO i. Sample Preparations, ii. Microbiological studies, iii. Filtration of aqueous solutions 2 O O O C 2 ONO 2 ONO 2 n Specifications Pore Size 0.22µm 0.45µm Diameter 13mm, 25mm, 47mm, Water Flow Rate (ml/min/cm²) at ΔP = 10psi, 20 C Retention Efficiency 0.22µm: LRV > 7 for br. diminuta Integrity Test Data Pore Size Wetting Fluid Bubble Point 0.22µm Water 52psi 3.65 kg/cm² 0.45µm Water 33psi 2.32 kg/cm² 0.8µm Water 14psi 0.98 kg/cm² Cellulose Nitrate Membrane (Ester)Filters GX22013CN Cellulose Nitrate, 0.2µm, 13mm, 100/Pk 595 GX22025CN Cellulose Nitrate, 0.2µm, 25mm, 100/Pk 675 GX22047CN Cellulose Nitrate, 0.2µm, 47mm, 100/Pk 895 GX45013CN Cellulose Nitrate, 0.45µm, 13mm, 100/Pk 595 GX45025CN Cellulose Nitrate, 0.45µm, 25mm, 100/Pk 675 GX45047CN Cellulose Nitrate, 0.45µm, 47mm, 100/Pk 895 GX12013CN Cellulose Nitrate, 1.2µm, 13mm,100/Pk 595 GX12025CN Cellulose Nitrate, 1.2µm, 25mm, 100/Pk 675 GX12047CN Cellulose Nitrate, 1.2µm, 47mm, 100/Pk, 895

4 284 Membrane Filters Cellulose Nitrate / Acetate Membrane Filters Gridded Cellulose Nitrate Membrane Disc Filters - GCN GCN membrane are made of Cellulose Nitrate, a material which assures effective retention with high flow rates. These membranes are useful for water microbiology. A grid is printed on the surface to facilitate counting of colonies. Features and Benefits 3 igh flow rates 3 Non-inhibiting ink grids 3 Validated for microbial recovery GCN membranes are useful for water microbiology. The printed grid eases the counting of bacterial colonies, micro colonies etc., and in no way influences the growth of bacteria. CN Grided Membrane Disc Filters GX22025CN-GS CN Grided, 0.2µm, 25mm, 100/Pk 1,350 GX22047CN-GS CN Grided, 0.2µm, 47mm, 100/Pk 1,620 GX45025CN-GS CN Grided, 0.45µm, 25mm, 100/Pk 1,350 GX45047CN-GS CN Grided, 0.45µm, 47mm, 100/Pk 2,696 Sterile Membrane Disc Filters GX22013CN-S Cellulose Nitrate, 0.2µm, 13mm, 100/Pk 845 GX22025CN-S Cellulose Nitrate, 0.2µm, 25mm, 100/Pk 925 GX22047CN-S Cellulose Nitrate, 0.2µm, 47mm, 100/Pk 1,075 GX45013CN-S Cellulose Nitrate, 0.45µm, 13mm, 100/Pk 845 GX45025CN-S Cellulose Nitrate, 0.45µm, 25mm, 100/Pk 925 GX45047CN-S Cellulose Nitrate, 0.45µm, 47mm, 100/Pk 1,075 GX08013CN-S Cellulose Nitrate, 0.8µm, 13mm, 100/Pk 845 GX12013CN-S Cellulose Nitrate, 1.2µm, 13mm, 100/Pk 845 GX12025CN-S Cellulose Nitrate, 1.2µm, 25mm, 100/Pk 925 GX12047CN-S Cellulose Nitrate, 1.2µm, 47mm, 100/Pk 1,075 Cellulose Acetate Membrane Filter Cellulose acetate membranes are fine pored film materials having pore sizes ranging from 0.2μm to 3μm and total porosity of 80-85%.These membranes are produced unsupported or supported as discs of various-diameters (13, 35 & 47mm). These membranes are used for filtration of microbiological, biological, physical-chemical research work & of various other samples for different applications. Sterile filtration of water, culture media, solutions containing antibiotics or proteins, cell harvesting. Features 3 Very low non-specific adsorption 3 Excellent thermal resistance 3 Validated using Brevundimonas dimimuta 3 Technical Advantages 3 Minimum loss of proteins, preservatives etc. Autoclavable at 121 C or 134 C 3 Dry heat sterilization possible; Reliable sterile filtration OCOC 3 O O O C 2 OCOC 3 C 2 OCOC 3 O O OCOC 3 Specifications n Adsorption BSA<10ug/cm 2 Bubble point for 0.2um=3.3bar, 0.45um=1.9bar Chemical compatibility Resistant to aqueous solutions, p 4-8, against most alcohols, hydrocarbons and oils Extractable with water Less than 1% Material Cellulose acetate Sterilization By autoclaving at 121 o C or 134 o C with g radiation, dry heat or ethylene oxide Sterilized filtration Filters with 0.2um pore size validated by bacteria challenge Tests. Thermal Stability MAX 180 o C Cellulose Acetate GX22013CA Cellulose Acetate, 0.2µm, 13mm, 100/Pk 1,945 GX22025CA Cellulose Acetate, 0.2µm, 25mm, 100/Pk 2,380 GX22047CA Cellulose Acetate, 0.2µm, 47mm, 100/Pk 3,455 GX45013CA Cellulose Acetate, 0.45µm, 13mm, 100/Pk 1,945 GX45025CA Cellulose Acetate, 0.45µm, 25mm, 100/Pk 2,380 GX45047CA Cellulose Acetate, 0.45µm, 47mm, 100/Pk 3,455 OCOC 3

5 285 Nylon-66 Membrane Filter - Type NN Nylon Membrane Disc Filters are double layered hydrophilic, non-media migrating, biologically inert, flexible, durable, plain white absolute membrane filters offering a wide chemical compatibility. Features and Benefits 3 Absolute retention 3 Wide Chemical Compatibility( Compatible to many solvents & alkali solutions - p Range No extractables). 3 igh heat resistance 3 ave better base of handling Specifications Pore Size 0.22µm 0.45µm Diameter 13mm, 25mm, 47mm, Water Flow Rate (ml/min/cm²) at ΔP = 10psi, 20 C Retention Efficiency 0.22µm: LRV > 7 for br. diminuta Sterilisation: Autoclavable at 121 C for 5 cycles Maximum Operating Temperature: 80 C continuous Maximum Operating Pressure: 3 Kg/cm² Biosafety: Passes the Biological tests for Class VI plastics as described in USP Extractables with Water: Within Limits as specified in USP Oxidizable Matter: Passes as per USP Integrity Test Data Pore Size Wetting Fluid Bubble Point 0.22µm Water 52psi 3.65 kg/cm² 0.45µm Water 33psi 2.32 kg/cm² Nylon-66 Membrane Disc Filter - Type NN NN Membrane Disc Filters are hydrophilic, biologically inert, non media migrating, plain white absolute membranes. Recommended for filtration, sterilisation & clarification of mobile Phase in PLC processes with aqueous, alkaline & organic samples. Features and Benefits 3 Wide Chemical Compatibility 3 Low extractables 3 PCL Certified - Assures that the filters do not add any artefacts to the sample. Membrane Filters Nylon Nylon Membrane Filters for the Filtration of Alkaline Solutions & Organic Solvents Membrane Disc Filter Type- NN GX22013NN Nylon-66, 0.2µm, 13mm, 100/Pk 675 GX22025NN Nylon-66, 0.2µm, 25mm, 100/Pk 950 GX22047NN Nylon-66, 0.2µm, 47mm, 100/Pk 1,225 GX45013NN Nylon-66, 0.45µm, 13mm, 100/Pk 675 GX45025NN Nylon-66, 0.45µm, 25mm, 100/Pk 950 GX45047NN Nylon-66, 0.45µm, 47mm, 100/Pk 1,225 Membrane Disc Filter Type - NN GX22013NN Nylon-66, 0.2µm, 13mm, 100/Pk 675 GX22025NN Nylon-66, 0.2µm, 25mm, 100/Pk 950 GX22047NN Nylon-66, 0.2µm, 47mm, 100/Pk 1,225 GX45013NN Nylon-66, 0.45µm, 13mm, 100/Pk 675 GX45025NN Nylon-66, 0.45µm, 25mm, 100/Pk 950 GX45047NN Nylon-66, 0.45µm, 47mm, 100/Pk 1,225

6 286 Nitrocellulose Membranes Transfer / Blotting Membrane Transfer/Blotting Mambranes Nitrocellulose Membranes These membranes are produced specially for life science research. They are used both for dot blot as well as gel transfer. They have a high binding capacity for proteins. These membranes are well adapted to all kinds of protein blotting systems e.g. semi dry, capillary or electro transfer. They can also be used for nucleic acids. NC membranes are available as an economical alternative for nucleic acid and protein blotting protocols. These nitrocellulose transfer membranes were the original matrices used in key Southern, colony/plaque, and protein protocols. NC contains mixed cellulose esters matrix with no surfactant to interfere with cell wall integrity during cell growth. Use these membranes for colony lifts. These are mixed cellulose esters with surfactants that improve wetability ability and handling during the transfer process. Both membranes have a high binding capacity for nucleic acids and proteins. They are suitable for routine transfers. Features and Benefits 3 igh binding capacities for proteins and nucleic acid molecules Minimum background 3 igh signal to noise ratio 3 Uniform and easy wetability 3 Can be blocked by normal blocking methods 3 Does not bind common protein stains 3 Compatible with colorimetric, radiolabelled, chemiluminescent, fluorescent and staining detection methods Protein blotting, nucleic acid dot/slot blots, colony/plaque lifts, enzyme immunoassays Specifications Type Pore Size Size SCN 0.22µm 0.45µm 82 mm circles 90 mm circles 142 mm circles 150 mm x 150 mm sheets 200 mm x 200 mm sheets 300 mm x 300 mm sheets 300mm x 3mtr. roll GX222020SCN NitroCellulose, 0.2µm, 200 x 200mm sheet, 25/Pk 12,500 GX223030SCN NitroCellulose, 0.2µm, 300 x 300mm sheet, 25/Pk 27,500 GX22082SCN NitroCellulose, 0.2µm, 82mm, 50/Pk 4,085 GX CN NitroCellulose, 0.2µm, 30cm x 3m, 1 roll 8,750 GX22090SCN NitroCellulose, 0.2µm, 90mm, 50/Pk 4,085 GX45090SCN NitroCellulose, 0.45µm, 90mm, 50/Pk 4,085 GX22142SCN NitroCellulose, 0.2µm, 142mm, 50/Pk 6,805 GX452020SCN NitroCellulose, 0.45µm, 200 x 200mm sheet, 25/Pk 12,500 GX221515SCN NitroCellulose, 0.2µm, 150 x 150mm sheet, 25/Pk 8,200 GX453030SCN NitroCellulose, 0.45µm, 300 x 300mm sheet, 25/Pk 27,500 GX CN NitroCellulose, 0.45µm, 30cm x 3m, 1 roll 8,750 Transfer Membrane Nitro Cellulose Membrane

7 287 Nylon 6,6 Blotting Membrane SNN membranes are pure Nylon-66 highly uniform membranes, specially useful for nucleic acid binding. Nylon membranes cast from nylon 6,6 polymer(1,2) have superior handling characteristics relative to nitrocellulose membranes and are better able to withstand the chemical conditions for stripping DNA or RNA probes. The use of nylon membranes in nucleic acid blotting applications has improved the performance and capabilities of hybridization-based assays.these are positively charged with 0.2um & 0.45um pore sizes. The open pore structure permits maximum accessibility of target sequences to the probe and allows efficient removal of unhybridized probes, thereby reducing background. Micro-Por range offers choice of transfer membranes for nucleic acid applications. For superior results in Southern blotting, Northern blotting, and colony/plaque lifts, positively charged Ny+ transfer membrane is recommended for Southern and colony plaque protocols, and is also available as a less sensitive and more economical alternative. Ny+positively charged nylon membrane optimized for reliable and reproducible transfer, immobilization, hybridization, and subsequent reprobing. The density and uniformity of the positively charged surface produces maximum sensitivity with minimal background. The membrane is reinforced for enhanced durability, which is especially important in reprobing experiments. Features and Benefits 3 Maximum transfer of bimolecules from the gel 3 Maximum binding and retention of the transferred biomolecules on the membrane throughout subsequent analyses 3 Maximum accessibility of all membrane-bound bimolecules to the probe 3 Maximum detection sensitivity and minimum background 3 Exceptional reprobing characteristics 3 Performs well in both chemiluminescent and radioactive detection systems Nucleic acid transfers, dots/slot blots, colony/plaque lifts and multiple reprobing Specifications Type Pore Size Size SNN 0.22µm 0.45µm 82 mm circles 90 mm circles 142 mm circles 150 mm x 150 mm sheets 200 mm x 200 mm sheets 300 mm x 300 mm sheets 300mm x 3mtr. roll Positively Charged Nylon Membrane GX22082BNN Nylon-66, 0.2µm, 82mm, 50/Pk 4,480 GX22090BNN Nylon-66, 0.2µm, 90mm, 50/Pk 4,480 GX221515BNN Nylon-66, 0.2µm, 15cm x 15cm, 25/Pk 17,550 GX222020BNN Nylon-66, 0.2µm, 20 x 20cm, 25/Pk 13,410 GX223030BNN Nylon-66, 0.2µm, 30 x 30cm, 25/Pk 29,769 GX BNN Nylon-66, 0.2µm, 82mm, 30cm x 3m, 1 Roll 10,800 GX452020BNN Nylon-66, 0.45µm, 20 x 20cm, 25/Pk 13,410 GX453030BNN Nylon-66, 0.45µm, 30 x 30cm, 25/Pk 29,769 GX BNN Nylon-66, 0.45µm, 30 x 30m, 1 Roll 10,800 Transfer/Blotting Mambranes Nylon 6,6 Blotting Membranes

8 288 Cellulose Acetate Syringe Filter PES (Poly Ethersulfone) Syringe Filters Cellilose Acetate Cellulose Acetate Syringe Filters show high flow rates and thermal stability with very low adsorption characteristics. Ideal for filtration of all kinds of biological samples for tissue culture applications, water & buffer samples, etc. Specification Retention Efficiency Wetability Sterilization Effective filtration area old up volume Extractables (NVR) Initial Water flow rate Filtration Volume 0.2 μm LRV > 7 for Brevundimonas diminuta 0.45 μm LRV > 7 for Serratia marcescens ydrophilic ETO 0.8 cm2 for 13mm & 3.9 cm2 for 25mm < 20 μl for 13 mm & < 100 μl for 25 mm (after air purge) Very low (with in limit as specified in USP XXIV) 38.4 ml/min for 0.2 μm & 72.3 ml/min for 0.45 μm (at 10 psi for 25 mm) 1-10ml for 13mm & ml for 25mm Minigen Cellulose Acetate Syringe filters, Sterile MG13020-CASL MG13045-CASL MG25020-CASL MG25045-CASL Minigen Cellulose Acetate Syringe Filters, Sterile, 0.20µm, 13mm, 1-10ml, 50/Pk 2,525 Minigen Cellulose Acetate Syringe Filters, Sterile, 0.45µm, 13mm, 1-10ml, 50/Pk 2,525 Minigen Cellulose Acetate Syringe Filters, Sterile, 0.20µm, 25mm, ml, 50/Pk 2,575 Minigen Cellulose Acetate Syringe Filters, Sterile, 0.45µm, 25mm, ml, 50/Pk 2,575 PES Syringe Filters are used for various laboratory filtration applications. These syringe filters are validated for retention efficiency, protein binding, chemical compatibility and extractables. Biological and pharmaceutical solutions can be filtered, in a wide p range of p2-12, because of their low protein adsorption shown by them. Specific Features 3 Low protein binding to minimize sample loss 3 Wide chemical compatibility 3 Low sample absorption 3 For Analytical and Research applications 3 Recommended for aqueous as well as organic solvent filtration 3 Filtration of difficult solutions Minigen Plus Syringe Filters, Sterile MG25020-CASGFL Minigen Plus Syringe Filters, Sterile, 0.2µm, 25mm, ml, 50/Pk 3,850 Minigen Cellulose Acetate Syringe filters, Non-Sterile MG13020-CABL Minigen Cellulose Acetate Syringe Filters, Non-Sterile, 00.20µm, 13mm, 1-10ml, 50/Pk 1,950 MG13045-CABL Minigen Cellulose Acetate Syringe Filters, Non-Sterile, 0.45µm, 13mm, 1-10ml, 50/Pk 1,950 MG25020-CABL Minigen Cellulose Acetate Syringe Filters, Non-Sterile, 0.20µm, 25mm, ml, 50/Pk 2,000 MG25045-CABL Minigen Cellulose Acetate Syringe Filters, Non-Sterile, 0.45µm, 25mm, ml, 50/Pk 2,000 Note : Non-Sterile, packed in transparent Plastic Jar (BULK PACK) Retention Efficiency 0.2 μm LRV > 7 for Brevundimonas diminuta 0.45 μm LRV > 7 for Serratia marcescens Wettability ydrophilic Sterilization ETO Effective filtration area 0.8 cm2 for 13mm & 3.9 cm2 for 25mm old up volume < 20 μl for 13 mm & < 100 μl for 25 mm (after air purge) Extractable (NVR) Very low (with in limit as specified in USP XXIV) Initial Water flow rate 46.2ml/min for 0.2 μm & 68.0ml/min for 0.45 μm (at 10 psi for 25 mm) Filtration Volume 1-10ml for 13mm & ml for 25mm Minigen PES Syringe Filters MG25022PESL Minigen PES Syringe Filters, 0.22µm, 25mm, ml, 50/Pk 3,000 MG25045PESL Minigen PES Syringe Filters, 0.45µm, 25mm, ml, 50/Pk 3,000

9 289 PVDF (Poly Vinylidene Fluoride) For fast sterile filtration, low binding, sterile syringe filters are used specifically for sterile filtration of aqueous and mild organic solvent. Supplied as individually packed and bulk packaged or bulk packed in a jar (non-sterile), surfactant-free PVDF membranes. Specifications Retention Efficiency Wettability Sterilization Effective filtration area old up volume Extractable (NVR) Initial Water flow rate Filtration Volume 0.2 μm LRV > 7 for Brevundimonas diminuta 0.45 μm LRV > 7 for Serratia marcescens ydrophilic ETO 0.8 cm2 for 13mm & 3.9 cm2 for 25mm < 20 μl for 13 mm & < 100 μl for 25 mm (after air purge) Very low (with in limit as specified in USP XXIV) 41.3ml/min for 0.2 μm & 94.3 ml/min for 0.45 μm (at 10 psi for 25 mm) 1-10ml for 13mm & ml for 25mm 3 For Analytical and Research applications 3 For aqueous as well as organic solvent filtration 3 Filtration of difficult solutions Minigen PVDF Syringe Filters, Sterile MG13020-PVDFSL Minigen PVDF Syringe Filters, Sterile, 0.20µm, 13mm, 1-10ml, 50/Pk 2,850 MG13045-PVDFSL Minigen PVDF Syringe Filters, Sterile, 0.45µm, 13mm, 1-10ml, 50/Pk 2,850 MG25020-PVDFSL Minigen PVDF Syringe Filters, Sterile, 0.20µm, 25mm, 1-10ml, 50/Pk 2,950 MG25045-PVDFSL Minigen PVDF Syringe Filters, Sterile, 0.45µm, 25mm, ml, 50/Pk 2,950 Minigen PVDF Syringe Filters, Non-Sterile MG13020-PVDFBL Minigen PVDF Syringe Filters, Non-Sterile, 0.20µm, 13mm, 1-10ml, 50/Pk 2,000 MG13045-PVDFBL Minigen PVDF Syringe Filters, Non-Sterile, 0.45µm, 13mm, 1-10ml, 50/Pk 2,000 MG25020-PVDFBL Minigen PVDF Syringe Filters, Non-Sterile, 0.20µm, 25mm, ml, 50/Pk 2,100 MG25045-PVDFBL Minigen PVDF Syringe Filters, Non-Sterile, 0.45µm, 25mm, ml, 50/Pk 2,100 Note : Non-Sterile, packed in a transparent Plastic Jar (BULK PACK) Nylon Nylon syringe filters are used in different analytical applications.ydrophilic in nature, suitable for aqueous or organic sample preparation for PLC<GC or dissolution sample analysis. With its excellent flow characteristics, very low extractable levels and mechanical stability nylon offers the best combination of physical parameters to meet stringent analytical needs. Available as sterile, individually packed or bulk packed in a jar (non-sterile), PP2 housing. 3 PLC Solvents Sample Filtration 3 Mobile phase separation 3 For aqueous as well as organic solvent filtration Retention Efficiency 0.2 μm LRV > 7 for Brevundimonas diminuta 0.45 μm LRV > 7 for Serratia marcescens Wettability ydrophilic Sterilization ETO Effective filtration area 0.1cm2 for 4mm, 0.8 cm2 for 13mm & 3.9 cm2 for 25 mm old up volume < 20 μl for 13 mm & < 100 μl for 25 mm (after air purge) Extractable (NVR) Very low (with in limit as specified in USP XXIV) Initial Water flow rate 35.4 ml/min for 0.2 μm & 67.2 ml/min for 0.45 μm (ml/min at 10 psi for 25 mm) Filtration Volume 1-10ml for 13mm & ml for 25mm Minigen NYLON Syringe Filters, Sterile MG13020-NYSL Minigen NYLON Syringe Filters, Sterile, 0.20µm, 13mm, 1-10ml, 50/Pk 2,525 MG13045-NYSL Minigen NYLON Syringe Filters, Sterile, 0.45µm, 13mm, 1-10ml, 50/Pk 2,525 MG25020-NYSL Minigen NYLON Syringe Filters, Sterile, 0.20µm, 25mm, ml, 50/Pk 2,575 MG25045-NYSL Minigen NYLON Syringe Filters, Sterile, 0.45µm, 25mm, ml, 50/Pk 2,575 Minigen NYLON Syringe Filters, Non-Sterile MG13020-NYBL Minigen NYLON Syringe Filters, Non-Sterile, 0.20µm, 13mm, 1-10ml, 50/Pk 1,950 MG13045-NYBL Minigen NYLON Syringe Filters, Non-Sterile, 0.45µm, 13mm, 1-10ml, 50/Pk 1,950 MG25020-NYBL Minigen NYLON Syringe Filters, Non-Sterile, 0.20µm, 25mm, ml, 50/Pk 2,000 MG25045-NYBL Minigen NYLON Syringe Filters, Non-Sterile, 0.45µm, 25mm, ml, 50/Pk 2,000 Syringe Filters PVDF & Nylon

10 290 Biostat A Your Professional Start to Controlled Cultivation Biostat B The Multi-Talent for Research and Process Development Biostat A is a minimum footprint and easy-to-use bioreactor fermenter designed as an entry-level model for microbial fermentation and cell culture. The Biostat B is our universal benchtop controller for stirred and rocking motion systems. l Microbial fermentation and cell culture l Academic education and technical training l Early-stage research and development l Microbial, insect and mammalian cell culture l Suspension and microcarrier cultivation l Process development l Process optimization l Process characterization With its compact design, Biostat A saves valuable space in your laboratory. If you are looking for a bioreactor for training purposes or for scale-up from shake flask to controlled cell growth, Biostat A is the perfect fit. Biostat A is available in configurations for microbial fermentation and for cell culture applications. Using the advanced package, you can operate it by a tablet or a smartphone and can perform fed-batch processes. With multiple thousand installations worldwide, the Biostat B is the market leading benchtop system for various R&D applications. One Biostat B control tower controls up to two culture vessels completely independently, saving valuable bench space. It is available with the UniVessel Glass in a range of 1 L, 2 L, 5 L and 10 L, as well as with the 2 L single-use UniVessel SU and with the RM Rocker in a choice of 20 L and 50 L.

11 291 Biostat Cplus The Stainless Steel Fermenter & Bioreactor for Your Laboratory Biostat D-DCU Your Fast Lane to Production Biostat Cplus is a fermenter bioreactor developed for the fermentation of microorganisms and cultivation of animal cells. Biostat D-DCU incorporates many advanced features and preferred options to meet virtually any requirements for leading-edge bioprocess applications. l Microbial and cell culture l Suspension and microcarrier cultures l Process development and scale-up l Seed production l Protein supply for research and development l Microbial and cell culture l Suspension and microcarrier cultures l Process development and scale-up l Seed production l Production The system can be flexibly integrated into an existing laboratory infrastructure. The culture vessel can be sterilized by electro or steam heating. The casters under the supply unit of the bioreactor enable it to be easily moved to another location. The Biostat Cplus is a floor standing version, available with a selection of culture vessels with working volumes of 10 L, 15 L, 20 L and 30 L. A benchtop version is available with a 5 L working volume culture vessel. Extended functionality and ease of use is provided by a wide choice of in-line sensors and anaylzers with an integrated display of process values on the DCU screen. It is designed to interface single-use storage bags for media addition and harvesting, as well as the TAKEONE single-use aseptic sampling systems. Further enhanced functionality and ease of use are provided by a wide selection of in-line sensors and analyzers and by integrated display of process values on the DCU screen. Due to the bioreactor s modular design, various configurations are available from basic to fully featured. The system offers an excellent solution for any budget and every need. Working volumes are available in a choice of 10 L, 20 L, 30 L, 50 L, 100 L and 200 L.

12 292 Product Description Price( ) CAT# Product Description Price( ) Integra Liquid andling System CAT# The peristaltic pump DOSE IT makes dispensing of culture media, buffers and other solutions easy and ef cient. Forget about complicated programming. Choose a mode, a preprogrammed protocol or simply combine the 7 different step types, edit their parameters according to your needs and store them as a customized protocol in one of the 20 available program slots. Website: