Simplifying Sample Prep for University Labs

Transcription

1 Simplifying Sample Prep for University Labs Products for chromatography, sterile filtration, molecular separation, protein purification, and transfer and immobilization

2 Simplifying Sample Prep for University Labs Pall Life Sciences develops and produces many different immobilization products designed to maximize processing membrane chemistries for a multitude of applications. accuracy and speed. Many other products are also available. This brochure will help you select from sterile filtration, If you do not see the item you are looking for, just ask. molecular separation, protein purification, and transfer and Sterile Acrodisc Syringe Filters Superior flow rate and higher throughput than competitive devices Low protein binding to minimize sample loss. Available with built-in prefilter for increased throughput of difficult-to-filter liquids (heavy particulate load). Easy to use luer lock fittings. Available in a variety of sizes to accomodate volumes from ml. Sterilized by gamma irradiation to eliminate potential contamination by EtO residuals. Filtration of cell and tissue culture media and additives. Clarification of biological fluid, protein, enzyme, probe and hybridization buffers, and other aqueous samples. Filtration of aqueous solutions. Filtration where low protein binding is desired. For cell cryopreservation, use DMSO-safe Acrodisc syringe filters that necessarily undergo EtO sterilization. Acrodisc Syringe Filters With Supor (Polyethersulfone) Membrane µm, 13 mm 75/pkg µm, 13 mm 75/pkg µm, 13 mm 75/pkg µm, 25 mm 50/pkg µm, 25 mm 50/pkg µm, 25 mm 50/pkg µm, 25 mm 50/pkg /0.2 µm, 25 mm 50/pkg µm, 32 mm 50/pkg µm, 32 mm 50/pkg µm, 32 mm 50/pkg /0.2 µm, 32 mm 50/pkg µm, 32 mm 50/pkg µm, 32 mm 50/pkg Serum Acrodisc Syringe Filter With Supor Membrane Glass fiber/0.2 µm, 37 mm 20/pkg DMSO-Safe Acrodisc Syringe Filter µm Nylon membrane, 25 mm 50/pkg 2

3 VacuCap Vacuum Filtration Devices Innovative bottle-top filters can fill multiple bottles with one device Accepts a variety of collection vessels* and reduces storage space and waste. Environmentally-friendly with minimal plastic waste. Draws directly from the mixing reservoir. Eliminates possibility of contamination from transfer steps by filtering directly into sterile container. Supor membrane provides high flow rates. Available with built-in prefilter to prevent clogging and to increase throughput of high-particulate solutions. Vacuum-driven filtration of cell and tissue culture media, microbiological media, aqueous solutions, protein solutions, and buffers. Prefiltration or clarification of aqueous solutions. PF version useful for filtration of hard-to-filter solutions. Instructions 1. Connect the feed tubing to the port marked INLET on the VacuCap device. Place the opposite end of the tubing in the unfiltered fluid to be drawn. 2. Connect the vacuum tubing to the port marked VACUUM on the VacuCap device. Refer to product insert for safety precautions. 3. While holding the VacuCap device securely on the filtrate container, start the vacuum. The VacuCap device will seal securely to the container top and fluid will be drawn. 4. When filtration is complete, switch off the vacuum pump allowing the vacuum inside the receiving container to dissipate. Refer to the product insert for complete instructions. VacuCap 60 Devices, Gamma Irradiated (100 ml-1 L) µm, 60 mm 10/pkg µm, 60 mm 10/pkg µm, 60 mm 10/pkg /0.2 µm, 60 mm 10/pkg TA µm, 60 mm (with individually 10/pkg attached tubing) VacuCap 90 Devices, Gamma Irradiated (1 L-5 L) µm, 90 mm 10/pkg µm, 90 mm 10/pkg µm, 90 mm 10/pkg /0.2 µm, 90 mm 10/pkg TA µm, 90 mm (with indivually 10/pkg attached tubing) * Always use bottles designed for use with vacuum. 3

4 Vent Devices Protect your cell culture and lab environment Acro 37 TF Vent Device µm PTFE membrane, 37 mm 24/pkg µm PTFE membrane, 37 mm 200/pkg Bacterial Air Vents µm (nominal) glass, 37 mm 24/pkg µm (nominal) glass, 37 mm, 10/pkg gamma-irradiated Designed to protect bioreactors, fermentation tanks, culture vessels, and carboy contents from external contamination and to protect the environment from contaminants within the vessel. Self-contained, compact filter devices provide high efficiency removal of airborne bacteria and particulate under dry or moist conditions. Vacushield vent devices should be used between pump and receiving vessels to protect the valves and pump components from damage by aqueous solutions and to prolong the life of the pump. Always select a filter with a sufficient air flow rate to accommodate the air flow required by each application. Bioreactors Fermentation tanks Isolation or environmental chambers Receiving vessels Carboys Other small containers Acro 50 Vent Devices With PTFE Membrane µm, hose barb 18/pkg µm, hose barb 18/pkg µm, hose barb 18/pkg µm, 1/8 in. MNPT 18/pkg Acro 50 Vent Devices With Emflon II Membrane A50V002P2 0.2 µm hydrophobic PVDF 3/pkg membrane, 50 mm Vacushield Vent Device mm, hose barb 3/pkg 4

5 BioTrace PVDF, BioTrace NT, and Biodyne Transfer Membranes Membranes for transfer and immobilization BioTrace PVDF (Hydrophobic Polyvinylidene Fluoride) Transfer Membranes Versatile membrane for nucleic acid and protein transfers. Low background with chemiluminescent detection systems. No discoloration. High tensile strength. BioTrace NT (Nitrocellulose)Transfer Membranes 100% pure nitrocellulose, no support fabrics to interfere with signal generation. High binding capacity for proteins and nucleic acids. Lower protein burnthrough than competitors in electrophoretic transfers. Biodyne (Nylon) Transfer Membranes Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing. Superior performance with radioactive (Biodyne B) and non-radioactive (Biodyne A) detection systems. High sensitivity and low background. BioTrace PVDF membrane is ideal for Western transfers, protein dot blots, and protein sequencing. Use BioTrace NT membrane for colony/plaque lifts and protein transfers. Biodyne membranes are suitable for nucleic acid applications, as well as applications requiring enhanced detection and resolution. BioTrace PVDF Transfer Membrane x 20 cm sheets 10/pkg cm x 3 m roll 1/pkg BioTrace NT Transfer Membrane x 20 cm sheets 10/pkg cm x 3 m roll 1/pkg Biodyne A Membrane, 0.45 µm x 20 cm sheets 10/pkg cm x 3 m roll 1/pkg Biodyne B Membrane, 0.45 µm x 20 cm sheets 10/pkg cm x 3 m roll 1/pkg Biodyne C Membrane, 0.45 µm x 20 cm sheets 10/pkg Biodyne Plus Membrane, 0.45 µm x 20 cm sheets 10/pkg cm x 3 m roll 1/pkg 5

6 Centrifugal Filtration Devices Ensure rapid processing of samples with typical recoveries greater than 90% Nanosep Centrifugal Devices Simple, reliable concentrating and desalting of 50 to 500 µl samples. Microsep Advance Centrifugal Devices Precise, quick recovery of microliter volumes of concentrate from starting volumes up to 5 ml. Macrosep Advance Centrifugal Devices Quickly concentrates up to 20 ml of biological sample without valuable sample loss. Jumbosep Centrifugal Devices Convenient and reliable concentration, purification, and diafiltration of 20 to 60 ml biological samples. Concentrate and desalt proteins. Exchange buffer and remove salt. Use Nanosep devices to clean up labeling and PCR reactions. Use Microsep Advance devices for protein and particulate removal from samples for HPLC analysis of drugs, amino acids, and antibodies. Use Macrosep Advance and Jumbosep devices to recover proteins or other molecules from cell culture supernatants. Nanosep Centrifugal Devices OD003C33 3K, gray 24/pkg OD010C33 10K, blue 24/pkg OD030C33 30K, red 24/pkg OD100C33 100K, clear 24/pkg Microsep Advance Centrifugal Devices MCP003C41 3K, gray 24/pkg MCP010C41 10K, blue 24/pkg MCP030C41 30K, red 24/pkg MCP100C41 100K, clear 24/pkg Macrosep Advance Centrifugal Devices MAP003C37 3K, gray 24/pkg MAP010C37 10K, blue 24/pkg MAP030C37 30K, red 24/pkg MAP100C37 100K, clear 24/pkg Jumbosep Centrifugal Device Membrane Inserts OD003C65 3K membrane insert, gray 12/pkg OD010C65 10K membrane insert, blue 12/pkg OD030C65 30K membrane insert, red 12/pkg OD100C65 100K membrane insert, clear 12/pkg 6

7 Minimate Tangential Flow Filtration System Streamline laboratory-scale concentration, desalting, and buffer exchange processes Minimate TFF System OAPMP V AC 50/60 Hz, includes 1/pkg peristalic pump, pump head, pressure gauge, reservoir, stir plate, drip tray, and assorted fittings System s plug-n-play design includes all the hardware, tubing, and fittings needed to get your TFF process up and running quickly. Concentration and diafiltration processes can be performed on the same system with minimal user intervention. Cost-effective design easy to clean and reuse. Minimate TFF Capsules With Omega Membrane Cat. No. Part No. Description (MWCO) Pkg OAD65C12 650D 1/pkg OA001C12 1K 1/pkg OA003C12 3K 1/pkg OA005C12 5K 1/pkg OA010C12 10K 1/pkg OA030C12 30K 1/pkg OA050C12 50K 1/pkg OA070C12 70K 1/pkg OA100C12 100K 1/pkg OA300C12 300K 1/pkg OA500C12 500K 1/pkg OA990C K 1/pkg Concentrate and desalt proteins, peptides, or nucleic acids (DNA, RNA, oligonucleotides). Recover antibodies or recombinant proteins from clarified cell culture media. Separate (fractionate) large from small biomolecules. Concentrate viruses or gene therapy vectors. Prepare samples prior to column chromatography. 7

8 AcroPrep Advance Filter Plates For high throughput sample prep and detection procedures Provides consistency in filtration times, as well as efficient sample and bead recovery. Available in a variety of membrane configurations, plate colors, well volumes, and outlet tip lengths. Plates are constructed from chemically-resistant, biologically-inert polypropylene. Serialized barcode label allows for use in automated tracking systems. Concentration, purification, and desalting of proteins and peptides. Bead-/resin-based applications. Gross fractionation and lysate clarification. DNA and plasmid purification. General filtration. AcroPrep Advance Filter Plates, 96-Well, 350 µl µm Supor membrane 10/pkg µm Supor membrane 10/pkg µm Supor membrane 10/pkg µm PP/PE non-woven media 10/pkg µm glass fiber 10/pkg For DNA binding 10/pkg µm glass fiber/0.2 µm Supor membrane 10/pkg µm glass fiber/1.2 µm Supor membrane 10/pkg Omega 3K MWCO 10/pkg Omega 10K MWCO 10/pkg Omega 30K MWCO 10/pkg Omega 100K MWCO 10/pkg µm PTFE membrane 10/pkg µm PTFE membrane 10/pkg For multiplex assays 10/pkg Mustang Q membrane 10/pkg Mustang S membrane 10/pkg AcroPrep Advance Filter Plates, 96-Well, 1 ml µm Supor membrane 5/pkg µm Supor membrane 5/pkg µm Supor membrane 5/pkg µm glass fiber 5/pkg For DNA binding 5/pkg µm glass fiber/0.2 µm Supor membrane 5/pkg µm PTFE membrane 5/pkg µm PTFE membrane 5/pkg Omega 3K MWCO 5/pkg Omega 10K MWCO 5/pkg Omega 30K MWCO 5/pkg Omega 100K MWCO 5/pkg Mustang Q membrane 5/pkg Mustang S membrane 5/pkg AcroPrep Advance Filter Plates, 96-Well, 2 ml µm glass fiber 5/pkg µm glass fiber/0.2 µm Supor membrane 5/pkg µm PTFE membrane 5/pkg µm PTFE membrane 5/pkg AcroPrep 384 Filter Plates, 100 µl Well, Natural Color K, Omega membrane, long tips 10/pkg K, Omega membrane, short tips 10/pkg K, Omega membrane, long tips 10/pkg K, Omega membrane, short tips 10/pkg K, Omega membrane, long tips 10/pkg K, Omega membrane, short tips 10/pkg µm GHP membrane, long tips 10/pkg µm GHP membrane, short tips 10/pkg µm glass fiber media, long tips 10/pkg µm glass fiber media, short tips 10/pkg µm Supor membrane, long tips 10/pkg µm Supor membrane, short tips 10/pkg 8

9 AcroSep Chromatography Columns and Sorbents Rapid, high capacity protein purification in a user-friendly format Gel-in-a-shell technology in ion exchange sorbents provide rapid protein purification with high capacity and good resolution. Affinity sorbents offer high purity products in single step purifications. Mixed-mode sorbents provide better selectivity in polishing steps. Column s luer lock inlet and outlet allows convenient use with syringe, pump, or automated chromatography system. User-friendly column design is color-coded and labeled by chemistry type. Screen multiple ion exchange chemistries for use in protein purification. Ideal for optimization studies of protein purification schemes using small sample volumes prior to scale-up. Efficient and reliable small-scale purification of proteins for structural, functional, and yield analysis. AcroSep Ion Exchange Chromatography Columns, 1 ml C001 CM Ceramic HyperD F, green 5/pkg C001 S Ceramic HyperD F, blue 5/pkg C001 Q Ceramic HyperD F, red 5/pkg C001 DEAE Ceramic HyperD F, orange 5/pkg IEXVP-C001 1 each: Q, S, CM, and DEAE 4/pkg Ceramic HyperD F, mixed AcroSep Chromatography Columns for Affinity Purification, 1 ml C001 Protein A Ceramic HyperD F, pearl 5/pkg C001 IMAC HyperCel, cobalt blue 5/pkg C001 Blue Trisacryl M, dark blue 5/pkg AcroSep Chromatography Columns for Mixed- Mode, 1 ml C001 MEP HyperCel, purple 5/pkg C001 HEA HyperCel, black 5/pkg C001 PPA HyperCel, yellow 5/pkg C001 SDR HyperD, natural 5/pkg Chromatography Sorbents CM Ceramic HyperD F 25 ml S Ceramic HyperD F 25 ml Q Ceramic HyperD F 25 ml DEAE Ceramic HyperD F 25 ml Protein A Ceramic HyperD F 25 ml IMAC HyperCel 25 ml SDR HyperD 25 ml MEP HyperCel 25 ml Trisacryl GF05 M 100 ml 9

10 Acrodisc Syringe Filters with GHP Membrane Universal filter for both organic and aqueous solutions in HPLC and UHPLC sample prep Versatile GHP (hydrophilic polypropylene) membrane for aqueous and aggressive organic solvent-based solutions. Acrodisc PSF syringe filters with GHP membrane extend HPLC column life up to 46 times. Certified low levels of UV-absorbing extractables for accurate analysis for HPLC/UHPLC. Easy filtration of particulate-laden samples with glass fiber prefilter version. 13 mm Acrodisc syringe filter with minispike configuration offers low hold-up and easy filtration into autosampler vials. Highly recommended for filtering HPLC/UHPLC samples and mobile phases. The Acrodisc PSF GxF syringe filter provides two to four times the throughput of standard prefilter devices for extremely viscous samples. Acrodisc Syringe Filters With GHP Membrane, 13 mm µm, minispike outlet 100/pkg, 300/cs µm, minispike outlet 100/pkg, 300/cs Acrodisc PSF Syringe Filters With GHP Membrane, 25 mm AP µm 50/pkg, 200/cs AP µm 50/pkg, 200/cs AP-4307 GxF/0.2 µm 50/pkg, 200/cs AP-4559 GxF/0.45 µm 50/pkg, 200/cs 10

11 Membranes and Hardware Innovative lab essentials to help your process run smoothly Stainless Steel Forceps Tips have a flat, smooth surface to avoid membrane filter damage. Choose traditional black or multi-colored finger grips. 47 mm Magnetic Filter Funnels No-leak magnetic seal allows one-hand operation. 150 ml size allows for easy fit into small autoclaves, and 500 ml size is ideal for filtration of large samples. For use with the Membrane Filter Technique. SolVac Filter Holder Versatile design fits most HPLC bottles, flasks, and containers. Eliminates the added steps of washing flasks and transferring mobile phase solvents from flask to reservoir. Ideal for removing contaminating particulate from mobile phase or other solutions. Membrane Disc Filters Compatible with solvent-based solutions. Individual S-pack mermbrane is certified for microbiological analysis. Vacushield Vent Device Protects pump from damage due to liquids. Protects laboratory personnel from contaminants. Stainless Steel Forceps Black grips 1/pkg Multi-colored grips 3/pkg 47 mm Magnetic Filter Funnels ml capacity 1/pkg ml capacity 1/pkg ml capacity with lid 1/pkg 47 mm Membrane Disc Filters, S-Packs, Gamma-Irradiated Supor PES membrane, 0.2 µm, grid 200/pkg GN-6 Metricel membrane, 0.45 µm, grid 200/pkg Metricel Black PES membrane, 0.45 µm, grid 200/pkg Metricel Black PES membrane, 0.8 µm, grid 200/pkg SolVac Filter Holder With 61 cm (2 ft.) feedline tubing, thumb 1/pkg clamp, sinker, vacuum port adapter, 2 membrane and 2 seal gaskets Membrane Disc Filters HPLC Mobile Phase and Solvent Filtration Membranes, 47 mm µm, GH Polypro (PP) membrane 100/pkg µm, GH Polypro (PP) membrane 100/pkg µm, TF (PTFE) membrane 100/pkg µm, TF (PTFE) membrane 100/pkg µm, FP Vericel (PVDF) membrane 100/pkg µm, FP Vericel (PVDF) membrane 100/pkg µm, Nylaflo (Nylon) membrane 100/pkg µm, Nylaflo (Nylon) membrane 100/pkg Vacushield Vent Device mm, hose barb 3/pkg 11

12 Ordering: Technical Assistance: Product Support: , Pall Corporation. Pall,, Acrodisc, AcroPrep, AcroSep, Biodyne, Bio-Inert, BioTrace, HyperCel, HyperD, Jumbosep, Macrosep, Metricel, Microsep, Minimate, Mustang, Nanosep, Nylaflo, Omega, SolVac, Supor, Trisacryl, Ultrogel, VacuCap, Vacushield, and Vericel are trademarks of Pall Corporation. Indicates a trademark registered in the USA. Filtration. Separation. Solution.SM is a service mark of Pall Corporation. 3/11, 10K, GN (PN33561) M Lit. No