Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK - Edinburgh SNBTS Edinburgh Clinical Laboratories Royal Infirmary Edinburgh Little France Crescent EH16 4SA Contact: Symon Lockhart Tel: +44 (0) Symon.lockhart@nhs.net Additional site activity performed away from the laboratory: Location details Activity Accident and Emergency RIE Obstetrics Theatre- RIE Main theatre recovery-rie Site activities performed away from the locations listed above: Location details Activity Royal Hospital for Sick Children Assessment Manager: CM 1 of 6
2 DETAIL OF ACCREDITATION HUMAN BODY FLUIDS Blood Blood Transfusion testing for the purpose of clinical diagnosis Blood Grouping A,B Rh: C, c, D, E, e, Kl Rh: C, c, D, E, e, Cw, K,k P1: procedure and manufacturers Fully automated BioRad- IH-1000 analyser (Column Agglutination) by Electronic SOP NATS CLS 058 Manual BioRad cassettes (Column Agglutination) By visual A,B Rh: Cw,C, c, D, E, e, K, k, Kp a, Kp b Jk a, Jk b, Fy a, Fy b, Lu a, Lu b M, N, S, s, Le a,le b, P1: SOP NAT CLS 038 Assessment Manager: CM Page 2 of 6
3 Venous blood plasma/serum Eluate prepared from patient s blood sample Blood Transfusion testing for the purpose of clinical diagnosis (cont d) Antibody Identification AB Rh: anti-c w, anti-c, anti-c, anti-d, anti-e, anti-e, Kell system: anti-k, anti-k, anti-kp a, anti-jk a, anti-jk b, anti-fy a, anti-fy b, anti-lu a, anti-m, anti-n, anti-s, anti-s, anti-le a, anti-le b, P1: anti-p Antibodies to rare red cell antigens Rh: anti-c w, anti-c, anti-c, anti-d, anti-e, anti-e, Kell system: anti-k, anti-k, anti-kp a, anti-jk a, anti-jk b, anti-fy a, anti-fy b, anti-lu a, anti-m, anti-n, anti-s, anti-s anti-le a, anti-le b Anti P1 procedure and manufacturers Fully automated BioRad IH1000 (Column Agglutination) by Electronic SOP - NATS CLS 058 And And SOP And Assessment Manager: CM Page 3 of 6
4 Absorbed Plasma Plasma Blood Transfusion testing for the purpose of clinical diagnosis (cont d) Antibody IIdentification Rh: anti-c w, anti-c, anti-c, anti-d, anti-e, anti-e, Kell system: anti-k, anti-k, anti-kp a, anti-jk a, anti-jk b, anti-fy a, anti-fy b, anti-lu a, anti-m, anti-n, anti-s, anti-s, anti-le a, anti-le b, P1: anti-p1 Compatibility testing of patients plasma with donor cells procedure and manufacturers Allo-absorption of antibodies (SOP ) NAT CLS 038 or Fully automated BioRad IH1000 (Column Agglutination) by Electronic SOP NATS CLS 058 Venous blood Direct Antiglobulin Test (DAT) Fully automated BioRad IH1000 (Column Agglutination) by Electronic SOP NATS CLS 058 Manual procedures by tube technology and visual detection. Assessment Manager: CM Page 4 of 6
5 Histocompatibility and Immunogenetics testing for the purpose of clinical diagnosis procedure and manufacturer s Whole Blood Foetal/maternal bleed estimation Kleihauer Acid Elution- microscopic detection SOP Whole Blood Whole Blood ( DNA) Serum HLA: HLA class I and II typing (HLA- A,B,C,DR,DQ,DP) HLA class I and II antibody screening HLA class I and II antibody identification Detection of antibodies to Platelet Factor-4 Confirmation of antibodies to Platelet factor 4 DNA extraction using QIAGEN EZ-1 automated extractor. SOP PCR-SSO using Luminex X-Map SOP , , , , Standard-SSP (Innotrain) SOP , , , LABScreen mixed (One Lambda) using Luminex X-Map SOP , , LABScreen Single Antigen (One Lambda) using Luminex X-Map SOP , , Heparin Induced Thrombocytopenia (HIT) testing using Biorad ID PAGIA Heparin PF4 kit for antibody screening SOP Lifecodes PF4 IgG ELISA for antibody confirmation SOP Assessment Manager: CM Page 5 of 6
6 Whole blood Histocompatibility and Immunogenics testing for the purpose of clinical diagnosis (cont d) procedure and manufactures FACS Canto II Flow Cytometer CD34 enumeration SOP , , Flow Cytometry donor-recipient crossmatching Complement dependent cytotoxicity (CDC) donor-recipient crossmatching SOP , SOP , , , , END Assessment Manager: CM Page 6 of 6
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Royal Liverpool and Broadgreen University Hospitals NHS Trust Liverpool Clinical Laboratories (LCL) Department of Histocompatibility and Immunogenetics
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