Biosecurity Sanitation and Pest Control
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1 Biosecurity Sanitation and Pest Control
2 Animal Biosecurity All measures used to control all known or unknown infections in laboratory animals. All measures taken to identify, contain, prevent, and eradicate known or unknown infections that may cause clinical disease or alter physiologic and behavioral responses or otherwise make the animals unsuitable for research. (Both from the ILAR guide, page 109)
3 Biosecurity Levels Axenic: Free of all known or measurable microorganisms. Gnotobiotic: Having a defined list of microorganisms.
4 Biosecurity Levels IVC Individually ventilated caging Specific Pathogen Free (SPF): Free of a defined list of microorganisms. Viral Antibody Free (VAF): Free of known viruses. Restricted Flora--Free of Opportunistic Organisms Conventional: Open-top caging. May be at one of the above levels or may have been exposed to microorganisms. Are always kept clean and healthy.
5 Levels Of Sanitation Clean Remove soil Requires soap to remove oils May need a descaler (acid wash) Remove 99% of bacteria Improve efficacy Sanitize Reduce microorganisms to an acceptable level Eliminates 99.9% of vegetative bacteria Disinfect Destroy infectious agents Eliminates 100% of vegetative bacteria. Sterilize Destroy all living organisms
6 Cleaning Dumping cages requires protection for personnel from aerosolized allergens and potential biohazards. Dump station Mask
7 Sanitizers Washers Prewash: Removes loose soil Wash: Hot water and soap to remove soil and oils Rinse: Removes soap and sanitizes using hot water Temperature Versus Time 180 F for 0.1 second (Industry standard) 170 F for 1.5 seconds Final rinse and dry
8 Tunnel Washer Cages travel on a conveyor belt through the tunnel. Different sections perform the different phases of the wash.
9 Rack Washer Nozzles on either side oscillate. Nozzle on top. No nozzle from floor.
10 Sterilizers Sterilization Autoclave: Moist heat, ~250ºF for minutes Oven: Dry heat, ~320ºF for 1 hour Autoclave Glass bead sterilizer for surgical nstruments
11 Sterilization Gamma irradiation UV Light (no penetration) Filtration
12 Choosing the Appropriate Chemical Methods Requirements Organisms present Level of disinfection Toxicity Animals Humans Corrosion
13 Factors Affecting Chemical Efficacy Method of application Sponge, mop, wipe Spray Immersion Fog
14 Factors Affecting Chemical Efficacy Dilution Follow directions Too little Reduces efficacy Too much Wasteful Potential for toxicity # of bacteria killed Toxicity Kill rate Concentration
15 Factors Affecting Chemical Efficacy Time of exposure Temperature Organic soil load Hard water salts ph Incompatible chemicals Shelf life always label with expiration date
16 Chemical Labels Whenever possible, leave chemicals in their original container. When mixing, diluting, or aliquoting, labels must include: Full name of the hazardous substance Appropriate hazard warnings (words or pictogram) Date prepared Expiration date Name (initials) of the person preparing the label.
17 Sanitizer/Disinfectant Generally effective against most bacteria Most are not effective against Micobacterium tuberculosis Generally effective against enveloped viruses Most are not effective against non-enveloped viruses Not effective against spores
18 Sanitizer/Disinfectant Used on equipment and animal quarters Quaternary Ammonia Excellent cleaning ability Sticky residue, must be rinsed Phenolics (pine cleaners) Good cleaning ability Sticky residue, must be rinsed Toxic to cats
19 Sanitizer/Disinfectant Used on skin & wounds Iodine Fair cleaning ability Inactivated by organic matter, heat and UV light Iodine is irritating, staining, and toxic Iodophors (povidone-iodine), like Betadine, are not Used as a surgical prep: Betadine scrub and solution Alcohol Poor cleaning ability Desiccant Doesn t leave a residue, rinse is not required Used as a rinse in the surgical prep Also used to remove water from surgical instruments
20 Sanitizer/Disinfectant Used on skin and equipment Chlorhexidine DNS Dilute Nolvasan Solution (3%): Topical disinfectant for skin lesions Chlorhexidine scrub and solution can be used as a surgical prep (like Betadine) Can also be used to clean instruments, surgical areas, and animal quarters.
21 Sanitizer/Disinfectant Bleach (sodium hypochlorite) Used on inanimate objects and to disinfect water (16 drops/gallon) Excellent, and cost effective, disinfectant but often misused Poor cleaning ability, low penetration, must wash items first Not stable at use dilutions Inactivated by organic matter as well as heat and UV light Corrosive to metals--rinse required
22 Disinfectant/Sterilant Must be able to kill all microorganism, including spores Difference between levels may be based on concentration and/or contact time. Not usually stable at use dilutions
23 Disinfectant/Sterilant Used to sterilize equipment Chlorine dioxide Poor cleaning ability Inactivated by organic material Corrosive to metal--rinse required Peroxygen Compounds (Paracetic acid and hydrogen peroxide) Fair Cleaning ability Corrosive to metal rinse required
24 Sterilant Aldehydes Used to sterilize empty rooms--aerosol Poor cleaning ability Requires a long contact time Inactivated by organic matter Toxic Irritating and carcinogenic
25 Validation and Monitoring Indicators Environmental screening Proves ongoing effectiveness of products and procedures Methods RODAC plates (Replicate Organism Detection and Counting) ATP detection test
26 Odor Control Unacceptable method Masking overwhelming olfactory receptors with a better smell Acceptable method Destruction of odor-causing organisms
27 Pest Control Identify the pests Rodents Insects Birds Reptiles
28 Pest Control Types of Control Available Build them out Monolithic construction Repair access points Cracks, door seals
29 Pest Control Types of Control Available Build them out Monolithic Barriers
30 Pest Control Types of Control Available Build them out Monolithic Barriers Sanitation Clean drains No standing water No clutter: cover material that can t be cleaned
31 Pest Control Program Pest control methods Traps: rodents, insects Bait stations: rodents Boric acid (powdered desiccant): insects Pesticide sprays: insects Pyrethrum Routine program Requires documentation Fly light
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