Biogal - Extensive Range of Unique Veterinary Diagnostic Solutions:

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2 Biogal Galed Labs. Experience -established in A leader in veterinary diagnostic kits. Serologic and direct evaluation of veterinary diseases. Customers > 30 countries.

3 Biogal - Extensive Range of Unique Veterinary Diagnostic Solutions: PCRun Molecular diagnostic, portable, fast, in clinic/lab ImmunoComb Portable, Compact, Fast Semi Quantitative ELISA ImmunoRun Portable, Fast Screening

4 Current PCRun Products Available 1. Canine Ehrlichia canis Molecular Detection Kit 2. Canine Anaplasma platys Molecular Detection Kit 3. Canine Babesia canis Molecular Detection Kit 4. Canine Babesia gibsoni Molecular Detection Kit 5. Canine Pathogenic Leptospira Molecular Detection Kit 6. Canine Leishmania infantum Molecular Detection Kit 7. Canine Parvo Virus Molecular Detection Kit 8. Feline Panleukopenia Virus Molecular Detection Kit 9. Feline Mycoplasma haemofelis Molecular Detection Kit 10. Feline FeLV DNA Provirus Molecular Detection Kit More Molecular Detection Kits in Development

5 Till now - Standard PCR PROCEDURE Requires multiple steps and equipment, Takes 3-4 hours Expensive

6 NOW Minimal and simple steps Hands On time 10 minutes No need for thermocycler Flexible - Can be used in lab and/or clinic Completed from start to results in 60 min

7 Biogal PCRun Advantages Vs Standard PCR Biogal PCRun Simple-no multiple steps Standard PCR Multiple steps Requires large thermocycler Portable less expensive, small heat block Thermocycler Expensive and large Fast turn-around time - 1 hour 1.User friendly 2.Easily learned 3.Performed by Vet Long turn-around time. 2-4 hours Performed by lab only.

8 PCR with PCRun 1. Extraction of DNA from sample blood, bone marrow, urine feces, swabs. (blood, bone marrow - collect in EDTA, citrate or heparin) PCRun Sample Prep or Commercial DNA extraction kit 2a. PCR reaction with extracted DNA - Amplification of target pathogen DNA PCRun Molecular Diagnostic Kit Heat Block or PCRun Reader 2b. Visualization of Results Ustar DNA Detection Device or PCRun Reader

9 PCRun Ingredients Primers* Mg ++ dntps Polymerase Light agent Freeze dried reagents

10 OPTION 1 USING HEAT BLOCK PCRun Sample Prep Components

11 Sample Prep OR

12 Sample Prep Option 1 Centrifuge

13 Sample Prep Option 2 Syringe

14 PCRun Reaction Kit Components

15 PCRun Reaction Kit Reaction Steps Adjust heat block to 60 0 C Disconnect required number of tubes Dispense 20µl DNA sample Incubate at room temperature for 1 min Heat Heat to 60 0 C for min min Analyze with DNA detection device

16 Displaying Diagnostic Results PCRun DNA Detection Device Lateral Flow Positive Negative

17 PCRun Nucleic Acid Detection Device Simple lateral flow technique. Result appears within 15 minutes. Can be performed easily in clinic or lab.

18 OPTION 2 USING PCRUN READER Sample Prep to be Used for PCRun Reader To be used with the PCRun Reader Biogal has experience with the Zymo sample prep DNA extraction kits. This kit can be purchased directly from Biogal or from Zymo. There are other DNA extraction kits compatible with the PCRun amplification kit, that can be used for the PCRun Reader. Biogal can advise which other kits are most suitable. Contact info@biogal.co.il for more information.

19 Amplification with PCRun Reader 15μl Adjust PCRun Reader to 60 0 c Disconnect the required number of tubes Dispense 15μl of PCRun Buffer Incubate at room temperature for 1 min 5μl Dispense 5μl of DNA sample Run test 60 min Note the results

20 PCRun Reader Very small, relatively inexpensive reader, simple to perform and read. Real Time PCR results Reaction can be viewed in the form of a graph or positive/negative on the screen.

21 PCRun Reader More Compact Real Time Thermocycler PCRun Reader Standard Thermocycler

22 Thank You! Biogal Galed Labs Acs Ltd. Tel: ,