Functional Cell-Based HTS Assays
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1 Functional ACTOne TM GPCR Cell-Based High-Throughput Screening Assays ACTOne TM GPCR assay is a live cell-based camp biosensor detection system generated for high-throughput functional screening of G-protein coupled receptors. Over 60 GPCR stable cell lines established and being developed Highly sensitive and big assay windows Excellent signal-to-noise ratio and reproducibility Real time kinetic assay or end point assay Work for both endogenous or recombinant GPCRs Agonist and antagonist screening in one assay Catalog Number ACTOne TM Stable Cell Lines for GPCRs, Gs Signaling Pathway CL-01-ADORA2A CL-01-ADORA2B CL-01-ADRB1 CL-01-ADRB2 CL-01-AMY3 CL-01-AVPR2 CL-01-CALCR CL-01-CALCRL CL-01-CRHR1 CL-01-CRHR2 CL-01-DRD1 CL-01-DRD5 CL-01-EP4R Adenosine A2A Receptor (ADORA2A) ACTOne TM Stable Cell Line Adenosine A2b Receptor (ADORA2B) ACTOne TM Stable Cell Line Beta-1 Adrenergic Receptor (ADRB1) ACTOne TM Stable Cell Line Beta-2 Adrenergic Receptor (ADRB1) ACTOne TM Stable Cell Line Amylin 3 Receptor (AMY3) ACTOne TM Stable Cell Line Arginine Vasopressin Receptor 2 (AVPR2) ACTOne TM Stable Cell Line Calcitonin Receptor (CALCR) ACTOne TM Stable Cell Line Calcitonin Receptor-like Receptor (CALCRL) ACTOne TM Stable Cell Line Corticotropin-Releasing Hormone Receptor 1 (CRHR1) ACTOne TM Stable Cell Line Corticotropin-Releasing Hormone Receptor 2 (CRHR2) ACTOne TM Stable Cell Line Dopamine Receptor D1 (DRD1) ACTOne TM Stable Cell Line Dopamine Receptor D5 (DRD5) ACTOne TM Stable Cell Line Prostaglandin E Receptor 4 (EP4R) ACTOne TM Stable Cell Line
2 Functional Catalog Number ACTOne TM Stable Cell Lines for GPCRs, Gs Signaling Pathway CL-01-FSHR CL-01-GCGR CL-01-GIPR CL-01-GLP1R CL-01-GLP2R CL-01-GPBAR1 CL-01-GPR119 CL-01-HTR4 CL-01-HTR6 CL-01-HTR7B CL-01-LHCGR CL-01-MC1R CL-01-MC3R CL-01-MC4R CL-01-MC5R CL-01-PAC1R CCL-01-PTGDR CL-01-PTGER2 CL-01-PTGIR CL-01-PTH1R CL-01-PTH2R CL-01-SCTR CL-01-TSHR CL-01-VIPR1 CL-01-VIPR2 Follicle-Stimulating Hormone Receptor (FSHR) ACTOne TM Stable Cell Line Glucagon Receptor (GCGR) ACTOne TM Stable Cell Line Gastric Inhibitory Polypeptide Receptor (GIPR) ACTOne TM Stable Cell Line Glucagon-like peptide 1 receptor (GLP1R) ACTOne TM Stable Cell Line Glucagon-Like Peptide 2 Receptor (GLP2R) ACTOne TM Stable Cell Line G-protein coupled bile acid receptor 1 (GPBAR1) ACTOne TM Stable Cell Line G-Protein coupled receptor 119 (GPR119) ACTOne TM Stable Cell Line 5 Hydroxytryptamine (Seroyonin) Receptor 4 (HTR4) ACTOne TM Stable Cell Line 5-Hydroxytryptamine (Serotonin) Receptor 6 (HTR6) ACTOne TM Stable Cell Line 5-Hydroxytryptamine (Serotonin) Receptor 7B (HTR7B) ACTOne TM Stable Cell Line Human Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) ACTOne TM Stable Cell Line Melanocortin 1 Receptor (MC1R) ACTOne TM Stable Cell Line Melanocortin 3 Receptor (MC3R) ACTOne TM Stable Cell Line Melanocortin 4 Receptor (MC4R) ACTOne TM Stable Cell Line Melanocortin 5 Receptor (MC5R) ACTOne TM Stable Cell Line Adenylate Cyclase Activating Polypeptide 1 Receptor (PAC1R) ACTOne TM Stable Cell Line Prostaglandin D2 Receptor (PTGDR) ACTOne TM Stable Cell Line Prostaglandin E2 Receptor (PTGER2) ACTOne TM Stable Cell Line Prostaglandin I2 Receptor (PTGIR) ACTOne TM Stable Cell Line Parathyroid Hormone 1 Receptor (PTH1R) ACTOne TM Stable Cell Line Parathyroid Hormone 2 Receptor (PTH2R) ACTOne TM Stable Cell Line Secretin Receptor (SCTR) ACTOne TM Stable Cell Line Thyroid Stimulating Hormone Receptor (TSHR) ACTOne TM Stable Cell Line Vasoactive Intestinal Peptide Receptor 1 (VIPR1) ACTOne TM Stable Cell Line Vasoactive Intestinal Peptide Receptor 2 (VIPR2) ACTOne TM Stable Cell Line
3 Functional Catalog Number ACTOne TM Stable Cell Lines for GPCRs, Gi Signaling Pathway CL-11-ADORA1 CL-11-ADORA3 CL-11-AGTRL1 CL-11-CB1 CL-11-CB2 CL-11-CCR5 CL-11-CXCR4 CL-11-DRD2 CL-11-GRM2 CL-11-GRM4 CL-11-GRM7 CL-11-GRM8 CL-11-HTR1A CL-11-HTR1F CL-11-MCHR1 CL-11-NPY1R CL-11-NPY2R CL-11-NPY4R CL-11-NPY5R CL-11-OPRD1 CL-11-OPRK1 CL-11-OPRL1 CL-11-OPRM1 CL-11-S1PR1 CL-11-SSTR5 Adenosine A1 Receptor (ADORA1) ACTOne TM Stable Cell Line Adenosine A3 Receptor (ADORA3) ACTOne TM Stable Cell Line Angiotensin II Recptor-like 1 (AGTRL1) ACTOne TM Stable Cell Line Cannabinoid Receptor 1 (CB1) ACTOne TM Stable Cell Line Cannabinoid Receptor 2 (CB2) ACTOne TM Stable Cell Line Chemokine (C-C Motif) Receptor 5 (CCR5) ACTOne TM Stable Cell Line Chemokine (C-X-C Motif) Receptor 4 (CXCR4) ACTOne TM Stable Cell Line Dopamine Receptor 2 (DRD2) ACTOne TM Stable Cell Line Metabotropic Glutamate Receptor 2 (GRM2) ACTOne TM Stable Cell Line Metabotropic Glutamate Receptor 4 (GRM4) ACTOne TM Stable Cell Line Metabotropic Glutamate Receptor 7 (GRM7) ACTOne TM Stable Cell Line Metabotropic Glutamate Receptor 8 (GRM8) ACTOne TM Stable Cell Line 5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A) ACTOne TM Stable Cell Line 5-Hydroxytryptamine (Serotonin) Receptor 1F (HTR1F) ACTOne TM Stable Cell Line Melanin-Concentrating Hormone Receptor 1 (MCHR1) ACTOne TM Stable Cell Line Neuropeptide Y Receptor Y1 (NPY1R) ACTOne TM Stable Cell Line Neuropeptide Y Receptor Y2 (NPY2R) ACTOne TM Stable Cell Line Neuropeptide Y Receptor Y4 (NPY4R) ACTOne TM Stable Cell Line Neuropeptide Y Receptor Y5 (NPY5R) ACTOne TM Stable Cell Line Opioid Receptor, Delta 1 (OPRD1) ACTOne TM Stable Cell Line Opioid Receptor Kappa 1 (OPRK1) ACTOne TM Stable Cell Line Opiate Receptor- like 1 (OPRL1) ACTOne TM Stable Cell Line Opioid Receptor, Mu 1 (OPRM1) ACTOne TM Stable Cell Line Sphingosine-1-phosphate Receptor 1 (S1PR1) ACTOne TM Stable Cell Line Somatostatin Receptor 5 (SSTR5) ACTOne TM Stable Cell Line Order:
4 AbPlus TM GPCR Cell-Based High-Throughput Screening Assays AbPlus TM HTS Sytem is a live cell-based assay platfrom for functional screening of Gi-coupled GPCR antibodies. This proprietary TRPC biosensor has been modulated specifically to detect Gi-coupled GPCR activity through a direct Gαi-TRPC interaction. Cell-based assay presents the epitope in its native conformation state More physiologically relevant assay conditions Real time kinetic assay or end point assay Highly sensitive, robust signal window and adaptable to various high throughput screen formats Well suited not only for agonist/antagonist screening, but also for allosteric regulator, partial agonist/ antagonist screening Catalog Number AbPlus TM Stable Cell Lines for Gi-coupled GPCRs CL-Ab04-CXCR4 CL-Ab04-GRM2 AbPlus TM Chemokine Receptor 4 (CXCR4) Stable Cell Line AbPlus TM Glutamate Metabotropic Receptor 2 (GRM2) Stable Cell Line Order:
5 ACTOne TM PDE Cell-Based High-Throughput Screening Assays Functional ACTOne TM PDE (Phosphodiesterase) assay is a live cell-based camp biosensor detection system using a constitutively active GPCR(Gs) as a driving force for camp production. Highly sensitive and big assay windows PDE inhibitor assays at near physiological conditions Real time kinetic assay or end point assay Work for both endogenous or recombinant PDEs Catalog Number ACTOne TM Cell Lines for Phosphodiesterase (PDE) CL-03-PDE10A CL-03-PDE1b CL-03-PDE1C CL-03-PDE4 CL-03-PDE2A CL-02-PDE1B CL-02-PDE4A CL-02-PDE4B CL-02-PDE4D Phosphodiesterase 10A (PDE10A) ACTOne TM Stable Cell Line (HEK293 cells) Phosphodiesterase 1B (PDE1B) ACTOne TM Stable Cell Line (HEK293 cells) Phosphodiesterase 1C (PDE1C) ACTOne TM Stable Cell Line (HEK293 cells) Phosphodiesterase 4 (PDE4) ACTOne TM Stable Cell Line (HEK293 cells) Phosphodiesterase 2A (PDE2A) ACTOne TM Stable Cell Line (HEK293 cells) Phosphodiesterase 1B (PDE1B) ACTOne TM Stable Cell Line (CHO cells) Phosphodiesterase 4A (PDE4A) ACTOne TM Stable Cell Line (CHO cells) Phosphodiesterase 4B (PDE4B) ACTOne TM Stable Cell Line (CHO cells) Phosphodiesterase 4D (PDE4D) ACTOne TM Stable Cell Line (CHO cells)
6 BioAssay Kits BioAssay kits for life science research Catalog Number Prooduct Size Cell Biology Assays: CA-A115 Elite TM ATP Assay Kit (luminescence) 1 plate (96-well) CA-C-061 Elite TM Caspase Activity Assay Kit (Green Fluorescence) 100 assays CA-C150 Elite TM Caspase 3/7 Assay Kit (Green Fluorescence) 200 assays CA-C158 Elite TM Caspase 8 Assay Kit (Fluorescence) 200 assays CA-C149 Elite TM Caspase 9 Assay Kit (Fluorescence) 200 assays CA-C066 Elite TM Cell Cytotoxicity Assay Kit (Red Fluorescence) 100 assays CA-C106 Elite TM Cell Cycle Assay Kit (Green Fluorescence) 100 assays CA-C124 Elite TM Live Cell Labeling Kit (Green Fluorescence) 10 plates (96-well) CA-C112 Elite TM Cell Viability Assay Kit (Green Fluorescence) 5 plates (96-well) CA-M145 Elite TM Mitochondrial Membrane Potential Kit 500 assays Ion Channel Assays: CA-C155 Elite Non-Wash Calcium Assay Kit 10 plates (96-well) CA-M165 Elite TM Membrane Potential Dye Kit (Fluorescent) 1000 assays CA-P217 Elite TM Potassium Ion Channel Assay Kit 10 plates Molecular & Biochemical Assays: CA-A401 Elite TM Acetylcholinesterase Assay Kit (Fluorescence) 200 assays CA-A403 Elite TM Acetylcholine Quantiation Kit (Fluorescence) 200 assays CA-A052 Elite TM Aldehyde Detection Assay Kit (Fluorescence) 200 assays CA-C060 Elite Calcium Quantitation Assay Kit 200 assays CA-C007 Elite TM Choline Quantitation Kit (Red Fluorescence) 200 assays CA-C315 Elite TM camp ELISA Assay Kit (Fluorescence) 1 plate (96-well) CA-C325 Elite TM camp 384-Well ELISA Assay Kit (Fluorescence) 1 plate (96-well) CA-F057 Elite TM Formaldehyde Detection Kit 200 assays CA-G005 Elite TM Glucose Colorimetric/Fluorometric Assay Kit 500 assays CA-G055 Elite TM GSH Assay Kit (Fluorescence) 200 assays CA-G056 Elite TM GSH/GSSG Ratio Assay Kit (Fluorescence) 200 assays CA-H100 Elite TM HDAC Activity Assay Kit (Green Fluorescence) 200 assays CA-H501 Elite TM Hydrogen Peroxide Detection Kit (Red Fluorescence) 200 assays CA-M340 Elite TM Multi-Drug Resistance Assay Kit 100 assays CA-N205 Elite TM NADH Assay Kit (Red Fluorescence) 400 assays CA-N218 Elite TM NAD/NADH Assay Kit (Red Fluorescence) 400 assays CA-N226 Elite TM NAD/NADH Ratio Assay Kit (Red Fluorescence) 250 assays
7 BioAssay Kits Catalog Number Product Size CA-N300 Elite TM Nitric Oxide (NO) Activity Assay Kit (Orange Fluorescence) 200 assays CA-N305 Elite TM Nitric Oxide (NO) Detection Kit (for Flow Cytometry) 200 assays CA-N325 Elite TM Nitric Oxide (NO) Detection Kit (Red Fluorescence) 200 assays CA-N405 Elite TM NADPH Assay Kit (Red Fluorescence) 400 assays CA-N418 Elite TM NADP/NADPH Assay Kit (Red Fluorescence) 400 assays CA-N426 Elite TM NADP/NADPH Ratio Assay Kit (Red Fluorescence) 250 assays CA-N602 Elite TM Neuraminidase (NA) Activity Assay Kit (Blue Fluorescence) 200 assays CA-P501 Elite TM Protease Activity Assay Kit (Red Fluorescence) 500 assays CA-R90 Elite TM Non-Wash ROS Activity Assay Kit (Green Fluorescence), H 2 O 2 preferred 200 assays CA-R911 Elite TM Non-Wash ROS Activity Assay Kit (Red Fluorescence), superoxide preferred 200 assays CA-R922 CA-R933 Elite TM Non-Wash ROS Activity Assay Kit (Orange Fluorescence), superoxide and hydroxyl radical preferred Elite TM Non-Wash Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence), superoxide and hydroxyl radical preferred 200 assays 200 assays CA-S005 Elite TM SOD Activity Assay Kit 200 assays ELISA Assay Kits for HIV-1 gp120 & Influenza A Hemagglutinin (HA): CA-gp120A Elite TM gp120 (HIV-1/Clade A) ELISA Assay Kit 200 assays CA-gp120B Elite TM gp120 (HIV-1/Clade B) ELISA Assay Kit 200 assays CA-gp120C Elite TM gp120 (HIV-1/Clade C) ELISA Assay Kit 200 assays CA-I157 Elite TM Hemagglutinin (H1N1)(A/Brisbane/59/2007) ELISA Assay Kit 200 assays CA-I179 Elite TM Hemagglutinin (H1N1)(A/California/07/09) ELISA Assay Kit 200 assays CA-I307 Elite TM Hemagglutinin (H3N2)(A/Brisbane/10/2007) ELISA Assay Kit 200 assays
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