ASE Series. Accelerated Solvent Extractors ASE 100/200/300. The World Leader In Accelerated Solvent Extraction
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1 ASE Series ASE 100/200/300 Accelerated Solvent Extractors The World Leader In Accelerated Solvent Extraction
2 Why is ASE the Best Extraction Technique? Rapid extraction time Accelerated Solvent Extraction (ASE ) reduces extraction times from hours to just minutes, dramatically reducing sample preparation time. Accomplish other tasks while ASE prepares your samples. Minimal solvent usage ASE significantly reduces the amount of solvent required to perform extractions, allowing you to save money and handle less solvent in your laboratory. Established and approved methods ASE methods have been established and accepted in the environmental, pharmaceutical, foods, polymer, and consumer products industries. ASE is accepted by the U.S. EPA under Method 3545A. What is ASE? Accelerated solvent extraction employs a combination of increased temperature and pressure with common solvents to increase the efficiency of the extraction process. The result? Faster extraction times and a significant reduction in solvent use. The benefits of using solvents at elevated temperature and pressure are: Higher analyte solubility Reduced matrix effects or weaker matrix and analyte interaction Faster analyte diffusion from matrix into solvent Decreased solvent viscosity for increased solvent penetration into the matrix Increased pressure to maintain solvent in a liquid phase during extraction What is the ASE procedure? After cells are filled with sample and loaded, ASE performs the following steps automatically: 1. Fills cell with solvent (organic or aqueous) 2. Heats and pressurizes the extraction cell 3. Holds sample at set pressure and temperature 4. Pumps clean solvent through sample 5. Purges sample with nitrogen The filtered extract, collected in the separate collection vial, is ready for analysis. Easy to use System programming and operation is quick and intuitive. The learning curve is minimal, so you ll be running samples in no time. Improved laboratory efficiency Increase your sample throughput and improve your lab efficiency with ASE. Minimize extrac tion times and increase sample throughput. The automation capabilities of the ASE 200 and 300 allow unattended operation, even overnight. 2
3 Pump Extractions in minutes Current extraction techniques can take up to 48 hours. With ASE, extractions are typically performed in 12 to 20 minutes. Oven Extraction Cell Technique Soxhlet Automated Soxhlet Sonication Microwave ASE Average Extraction Times 4 to 48 hours 1 to 4 hours 30 minutes to 1 hour 30 minutes to 1 hour 12 to 20 minutes Solvent savings ASE provides the lowest solvent use of any extraction technique. Solvent Technique Soxhlet Automated Soxhlet Sonication Microwave ASE Average Solvent Usage 200 to 500 ml 50 to 100 ml 150 to 200 ml 25 to 50 ml 15 to 45 ml Collection Bottle 3
4 ASE Meets Your Application Requirements Environmental For environmental applications, ASE has been compared extensively to existing techniques and found to produce data equivalent to or better than traditional methods. ASE is accepted for use in EPA Method SW A for the extraction of: Pesticides and herbicides PAHs and semi-volatiles PCBs Dioxins and furans TPH (DRO) Explosives More recently, ASE has been found to be effective for extracting organic compounds from air using polyurethane foam filters (PUF) and XAD resins. Food For the analysis of foods, ASE is used to: Monitor pesticide residues in a variety of sample types marketed for human or animal consumption. Determine the fat and/or additive content of many food types in accordance with new and more stringent labeling requirements. Determine the flavor profile of consumer products. The advantages that ASE offers for food analysis are speed and solvent savings. In addition, the very low detection levels required for the analysis of food products necessitates large sample volumes. With 100-mL extraction cells, ASE is capable of Analyte Class (EPA Analysis Method) MDL a Accuracy Recovery Precision µg/kg (% Recovery as a % of (%RSD) of CRM) Soxhlet Organochlorine pesticides (8081) (average of 20 compounds) PCBs (Aroclor 1254, 8082) Total Petroleum Hydrocarbons (DRO, 8015) NA 9.7 Organophosphorus Pesticides (8141) (average of 24 compounds) Chlorinated Herbicides (8151) c 15.5 (average of 8 compounds) Semivolatiles (BNAs, 8270) (average of 56 compounds) Dioxins (8280/8290) Low ppt 73 b 96 b 4.24 d a Calculated as per SW-846 chapter 1. b Average recovery of surrogates c Shaker method d Average RSD of Congeners extracting large gram-weight samples of both wet and dry samples. For agricultural products, ASE allows the automated extraction of residual pesticides (chlorinated and phosphorous), PCBs, and dioxins. Percent Recovery of Organophosphorus Pesticides from 30-g Apple Puree Sample Fortified at 50 ppb Pesticides % SD RSD % Recovery Dichlorvos/Naled Mevinphos TEPP Demeton-O (Ethoprop) Sulfotep Phorate Demeton-S Dimethoate Diazinon Disulfoton Parathion-methyl Fenchlorphos Malathion Fenthion Chlorpyrifos Parathion-ethyl Trichloronat Tetrachlorvinphos Prothiofos Merphos Fensulfothion Sulprofos EPN Azinphos-methyl Coumaphos n=12 Extraction for Marker Compou Dianthrones Deacylsaponins Silybi (St. John s Wort) (Horse Chestnut) (Milk Method Soxhlet ASE Soxhlet ASE Soxhle & Reflux Percent wt (RSD%) (7.1) a (2.9) (12) (5.4) (3.5) Solvents DCM DCM DCM DCM Petrol Acetone MeOH MeOH MeOH MeOH Volume 250 ml <50 ml 170 ml <50 ml 200 ml Total 38 h <25 min 7 h <40 min 9 h Time a RSD (%), n=3 4
5 Pharmaceuticals and Natural Products For pharmaceuticals, natural products, and nutraceuticals, ASE is used to: Extract natural products from plants Verify that nutritional supplements such as St. John s Wort, echinacea, and ginko biloba meet industry guidelines for standardized marker compound levels Monitor the level of pharmaceutical agents and their metabolites in animal tissues, and test stability Verify that levels of active compounds in products such as transdermal patches are within specification For these types of applications, ASE saves time and increases efficiency. Automation and improved reproducibility result in better control of manufacturing processes and faster identification of potential therapeutic agents. The ability to extract samples with a variety of different polarity solvents results in the generation of selective extract profiles, which then can be screened more easily for activity. Polymers In the polymer industry, ASE is used for: Characterization of actual polymer structures Extraction of plasticizers from PVC Extraction of polypropylene and polyethylene for additives such as UV stabilizers and antioxidants and slip agents Extraction of oils and organic acids from styrene butadiene rubber (SBR) samples Weight Percent of Each Plasticizer in PVC Plasticizer ASE Recovery (n=3) Soxhlet Recovery ASE Recovery (ASTM D2124) (n=2) (% vs. Target value) DOA TOP DOP TOTM Total Extractables from SBR Sample Target Value ASE Value (%) ASE RSD ASE Recovery (n = 3 ) (% vs. Target value) % % % % 98.9 ASE is used to replace hour solvent reflux extractions, thereby greatly reducing the extraction time and solvent exposure to lab personnel. In addition, faster turnaround of key QC information to the batch production facility can prevent loss of product due to poor formulation. ASE can also be used for competitive products analysis and routine product quality testing. nds from Natural Products n Curcumin Thymol Thistle) (Tumeric Rhizome) (Thyme) t ASE Reflux ASE Steam ASE Distillation (3.4) (2.2) (0.94) (7.0) (3.4) Hexane Hexane MeOH MeOH MeOH Water DCM <70 ml 50 ml <20 ml 250 ml <80 ml <25 min 1 h <30 min 2 h <25 min Extraction of Additives from Low Density Polyethylene (LDPE) Procedure Additive Concentration (ppm) I-3114 I-1010 I-1330 I-168 I-1076 Chloroform dissolution nd ASE nd Extraction of Additives from High Density Polyethylene (HDPE) Procedure Additive Concentration (ppm) I-3114 I-1010 I-1330 I-168 I-1076 ASTM D nd nd 240 ASE nd nd 281 5
6 Choosing the Right ASE System Which ASE is right for me? The three Dionex ASE systems are designed to meet the extraction requirements of many types of laboratories. Choose which system is best for yours: ASE <40 samples/week any sample size >40 samples/week sample size <30 g >40 samples/week sample size >30 g All ASE systems features: Automated sample extraction Automatic extract filtration Easy-to-fill sample cells with finger- or hand-tight fittings Easy-to-use collection vials and bottles Convenient front panel operation with multiple method storage Sensors for temperature, pressure, and solvent vapors ensure safe operation at all times Easy method transfer between systems Patented technology (patent numbers 5,843,311; 5,647,976; 5,660,727; and 5,785,856) Established methodologies and EPA approval Temperature range from ambient to 200 C ASE 100 Accelerated Solvent Extractor The ASE 100 is the entry-level ASE system designed for use in lower-throughput labs. This system is priced economically and offers fast and efficient extraction for a large range of sample sizes. Automated extraction of a single sample Economically priced for lower throughput laboratories Sample cell sizes: 10, 34, 66, and 100 ml Collection bottle 250 ml Operating pressure: 1500 psi (100 bar) Small footprint requires less than 36 cm (14 in.) of bench space ASE 200 Accelerated Solvent Extractor The ASE 200 is designed for high-throughput labs with sample sizes of 1 to 30 grams. With automation capabilities for up to 24 samples, the ASE 200 maximizes sample throughput and extraction efficiency. The ASE 200 is ideal for standard environmental analysis, pharmaceutical processes, and routine food and polymer applications. Unattended extraction of up to 24 samples Samples cell sizes: 1, 5, 11, 22, and 33 ml Scheduling programming for automated method optimization Collection vial sizes: 40 and 60 ml Automatic rinsing of system between sample extractions Compatible with AutoASE Computer Control Software and ASE Solvent Controller Operating pressure: psi ( bar) ASE 300 Accelerated Solvent Extractor The ASE 300 is designed for high throughput labs with large sample volume requirements. With automation capabilities of up to 12 cells and sample cell volumes up to 100 ml, the ASE 300 is ideal for the busy environmental and food analysis lab. Unattended extraction of up to 12 samples Samples cell sizes: 34, 66, and 100 ml 250-mL Collection bottles Automatic rinsing of system between sample extractions Scheduling programming for automated method optimization Compatible with AutoASE Computer Control Software and ASE Solvent Controller Operating pressure: 1500 psi (100 bar) 6 ASE 100 ASE 200 ASE 300
7 Automation Tools to Speed and Simplify Extractions ASE Solvent Controller The ASE Solvent Controller provides convenience and flexibility to method development and routine operation. For ASE 200 and ASE 300 the solvent controller is an ideal system addition both for users developing new methods and for those who must run many different types of analysis on a routine basis. Allows unattended, automatic switching between solvent systems. Solvent is delivered from one to four reservoirs with mixing from 5% to 95% of total volume. Reduces operator exposure to solvent by eliminating frequent solvent bottle changes between different sample types. Solvent caddy holds up to four 1-L or 2-L solvent bottles, and its reservoir contains and directs spills to waste. The ASE Solvent Controller is directly programmable from the ASE 200 or ASE 300. Multiple extractions can be performed using any combination of solvent sources. Alternate solvents can be used for rinsing the system. The AutoASE Main Screen displays current operating information, such as cell size, total solvent volume, solvents used, and actual temperatures and pressures. The schematic shows the operator what steps the ASE is performing. For example, solvent lines light up and an oven radiates when in use. AutoASE Computer Control Software for Greater Versatility and Power AutoASE is the control and reporting application software for ASE systems. AutoASE is advantageous for users with multiple systems or require printing and GLP compliance capabilities. Product Highlights Easy operator interface with ASE 200 and ASE 300 systems Sample information stored and tracked electronically Variable rinse volumes Allows text and numeric sample identifiers Easy-to-use icon bar for quick access to control functions Color graphic display of extraction process Up to eight ASE systems can be controlled or monitored from one PC, making it easier to monitor multiple system operation and transfer methods between systems Printer capabilities Windows 2000, NT, or XP ASE Solvent Controller 7
8 SYSTEM SPECIFICATIONS FOR ASE 100/200/300 ASE 100 Dimensions (h x w x d): 55.6 x 35.6 x 47.5 cm (21.9 x 14.0 x 18.7 in.) Weight: 34 kg (75 lb) ASE 200 Dimensions (h x w x d): 59.4 x 58.8 x 60.4 cm (23.4 x 23.1 x 23.8 in) Weight: 71.6 kg (156 lb) ASE 300 Dimensions (h x w x d): 59.4 x 58.8 x 60.4 cm (23.4 x 23.1 x 23.8 in) Weight: 75.2 kg (165.4 lb) ASE 100/200/300 Power Requirements: Consumption: 500 VA watts max. Voltage: or VAC Frequency: 50/60 Hz Pneumatic Requirements: N2 at kpa ( psi) Air at kpa ( psi) (optional) Corporate Headquarters Dionex Corporation 1228 Titan Way P.O. Box 3603 Sunnyvale, CA Tel: (408) Fax: (408) Worldwide Sales and Service North America U.S. (847) Canada (905) South America Brazil (55) Europe Austria (43) Benelux (31) (32) Denmark (45) France (33) Germany (49) Ireland (353) Italy (39) Switzerland (41) United Kingdom (44) Asia Pacific Australia (61) China (852) India (91) Japan (81) Korea (82) Singapore (65) Dionex products are designed, developed, and manufactured under an ISO 9001 Quality System Dionex Corporation Analyst is a registered trademark of Applied Biosystems. Hystar is a trademark of Bruker Biosystems. FastLoc, MSQ, and Xcalibur are trademarks of Thermo Electron Corporation. All other trademarks and registered trademarks are the property of Dionex Corporation. LPN M 06/07 Printed in U.S.A. Passion. Power. Productivity.
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