Ultra pure water, 18,2 MΩ cm. TKA GenPure. For Life Sciences, Analyses and Laboratory applications WATER PURIFICATION SYSTEMS

Transcription

1 Ultra pure water, 18,2 MΩ cm. TKA GenPure. TKA xcad. For Life Sciences, Analyses and Laboratory applications WATER PURIFICATION SYSTEMS

2 TKA GenPure. TKA xcad. Ultra pure water 18,2 MΩ cm, TOC 1 5 ppb. Assured quality. Even for the most demanding and sensitive applications. In the Biosciences For molecular biology and microbiology work In the preparation of cell and tissue cultures In PCR, DNA sequencing Electrophoresis In Analytical Chemistry Organic and inorganic trace analysis HPLC GC-MS, ICP-MS, GF-AAS TOC Measurements, IC In Routine Laboratory Work Analytical procedures IC, AAS, ICP-ES Norms All TKA Genpure systems exceed the norms ASTM type I, ISO 3696 Grade 1, ASTM D 1193, CLSI.

3 Made-to-measure performance. The right system for any requirements. TKA GenPure TKA GenPure is a system to produce Type I, reagent-grade water suitable for all general laboratory applications. TKA GenPure UF With ultrafiltration for pyrogen- and nuclease-free water. TKA GenPure UV With 185/254 nm UV photooxidation for the reduction of organic compounds. Each system can be optionally installed space-savingly under the bench or in a lab cupboard, whereby up to three control/dispenser units can be connected at a distance from it, with convenient control at each pointof-use. TKA GenPure UV/UF With a combination of ultrafiltration and UV photooxidation. TKA GenPure UV/TOC With UV photooxidation and TOC value display. TKA GenPure UV/TOC/UF With 185/254 nm UV photooxidation, ultrafiltration and TOC value display. All TKA systems can be qualified acc. to GLP and USP 30 <645> and <643>. TKA xcad Control And Dispenser unit. Gives you more elbow-room on the lab bench. Usable as bench-top or wall mounted system. Provides more flexibility to ultra pure water dispensing! See pages 6 and 7 for more details.

4 Intelligent technology that simplifies your work. Day for day! Optimum System Performance! Visible ultra pure water quality. The measurements of conductivity and real-time TOC value are made directly prior to the dispensing valve. The display shows the most important measured values during ultra pure water dispensing. For example: The display shows Non-Stop: System ready for ultra pure water dispensing 1 ppb: TOC value of the ultra pure water µs/cm: Conductivity of the ultra pure water 0.01 Liter: Adjustable volume to be dispensed 22.7 C: Temperature measured for conductivity compensation UV/TC: UV lamp and temperature compensation switched on All other display values and limiting value settings can be called from the menu by keystroke. Limiting values can be individually set, protected from unauthorized access. A fault message to the operator signals any overrun. Trends and faults can so be recognized early, allowing appropriate measures such as cleaning and disinfection to be immediately carried out. Automatic interval switching of the pump ensures regular recirculation of system-held ultra pure water through the purification circuit and UV photooxidation. Water remains clean and free from recontamination. Fully automatic disinfection of all internal components that contact water. 4

5 With the greatest accuracy! With much comfort! Measurement of conductivity By two high precision measuring cells. With fully automatic check and calibration prior to each measurement via built-in reference resistance. Temperature measurement By a platinum chip temperature sensor of +/- 0.1 C accuracy. With temperature compensation that can be switched off acc. to USP <645>. Online TOC measurement at the dispensing point Real time TOC monitoring for continual measurement of organic substances in the water acc. to USP 30 <643>. Intensity monitoring A photosensor continually checks the intensity of the UV lamp. A decrease in the measured UV radiation that would lead to an incorrect TOC measurement result is directly indicated in %. The display shows UV time UV Lamp operating h: time UV intensity UV Intensity comparison 96 % value Easily readable The ergometrically designed control/dispenser unit can be tilted for optimal key pressing and dazzle-free readability. Illuminated four-line alphanumeric display. Display of conductivity (in µs/cm) or specific electrical resistance (in MWxcm). Simple to use Menu-driven operation with clear text displays of all functions and performance parameters. Choice of English, German or French. Exact dosing High precision PVDF drawoff valve for dispensing with sterile filter Electronic dosing programme for fully automatic volume control of litres. More than 30 cm free space for dispensing, even suitable for high vessels. Fully automatic functioning Digital microprocessor control for automatic monitoring and control, with storage of faults from the last four weeks. Integrated in the operating and control unit, protection type IP 54. Real-time clock and code protected operating system prevent unauthorized changes and settings. GLP Documentation RS 232 Interface with adjustable sending intervals, for safe data transmission of all measured values and faults, with date and time, to a PC or printer. Rapid cartridge replacement The Aquastop quick-connects of the ultra pure water set allow replacement within seconds during operation. 5

6 TKA GenPure. TKA xcad. For flexible operation in the lab! No one laboratory is quite like the other and the jobs to be done are also different, just as are the wants for the place and position of an ultra pure water system that is to supply one or more workplaces. GenPure offers here a wide spectrum of set-up possibilities for the optimum choice. Ultra pure water. There where you need it. With a footprint about the size of an A 4 portrait, GenPure can be stood on the lab bench or, if preferable, it can be hung on a wall (without the need for extra mounting material). The space-saving under bench version can have up to three flexible control/dispenser units fitted to it for use at up to 3 m distance from it. 6

7 TKA xcad: extended Control And Dispenser unit High performance in compact format! Leaves more elbow-room on the lab bench! Can be stood on a bench or be even more space-savingly wall mounted Inclinable operator module for practical handling and good viewing Complete system control and steering via the operator module Turnable and height adjustable dispenser arm with extendable joystick. Action radius 80 cm Ergonomically shaped dispensing pistol provides highly precise volume dosing at the press of a button 7

8 Economical. By long-lasting replacement parts. UV Photooxidation 185/254! An optional, high performance UV unit serves for a reliable reduction in the content of microorganisms and their metabolites. The top-grade UV lamp is of synthetic quartz glass for the greatest light yield of exactly the right wavelengths. It is held in a robust and corrosion-resistant ANSI 316 L stainless steel housing. Integrated feedwater monitoring Good quality feedwater is a basic requirement for the degree of purity of the water produced, as well as for the long service lives of all purification stages. An additional measuring cell permanently monitors the conductivity of the feedwater. Any exceeding of the limiting value is immediately displayed. The feedwater must always be included in a proper validation of an ultra pure water system. Intensity monitoring included! The intensity of the the UV radiation is permanently monitored and displayed in %. The UV lamp must therefore only be replaced when really necessary. Long-lasting ultrafilter! A high efficiency ultrafilter reduces the level of endotoxines. The large ultrafiltration area and the surfacecleaning automatic rinse ensure both a high flow rate and a long service life. Powerful ultra pure water set! The ultra pure water set is very generously dimensioned. It so combines a long service life with cost-saving. The display gives notice of when it should be replaced. 8

9 TKA Technology. TKA Technology effectively combines various highly efficient purification stages and so minimizes organic and inorganic compounds to their detection limits. Flow chart: TKA GenPure UV-TOC/UF UV Photooxidation UV photooxidation reactor with specific geometry for even distribution of UV light of 185 nm and 254 nm wavelengths. 254 nm UV light kills bacteria by destroying the DNA in their cells. The bacterial content within the system is minimized. The combination of the two wavelengths produces hydroxyl free radicals that are extremely reactive. They oxidize and cleave small organic substances that are otherwise difficult to remove. The cleavage products are removed in the next purification stage. Adsorption + Ion exchange Purification set with a combination of high purity adsorptive material and an ion exchanger. The quality attained is monitored and displayed by continuous measurements of TOC and conductivity. The combination of adsorption and ion exchange removes the smallest organic molecules, such as the UV photooxidation cleavage products, as well as remaining inorganic ions. Ultrafiltration The asymmetric structure of the polysulphone ultrafilter and the tangential flow across the surface of it ensure a long sevice life. Retained substances are led to drain with the concentrate flow. Ultrafiltration separates off suspended and dissolved high molecular weight water compounds. It predominately serves here, however, to produce ultra pure water free of endotoxines and nucleases. Membrane filter Validated sterile filter of 0.2 µm pore size in a large area capsule with vent valve and protective bell. Sterile filter for the reliable removal of bacteria and particles directly prior to ultra pure water dispensing. Suitable for sterilization. The result: Ultra pure water of ASTM Type 1 quality. Conductivity in µs/cm: Resistivity, MWxcm at 25 C: 18.2 TOC value in ppb: 1 5 Bacteria in CFU/ml: < 1 Particulates, > 0,22 µm/ml < 1 Endotoxines in EU/ml: RNase in ng/ml: DNase in pg/µl: Flow rate: Up to 2 l/min 9

10 TKA GenPure. TKA xcad Technical specifications Applications: TKA GenPure TKA GenPure TKA GenPure TKA GenPure TKA GenPure TKA GenPure UF UV UF/UV UV-TOC UV-TOC/UF AAS, IC, ICP, standard buffer Molecular biology, microbiology, PCR, IVF, monoclonal antibodies Inorganic and organic trace analysis, HPLC, ICP-MS, IC, TOC analysis Molecular biology, PCR, DNA, monoclonal antibodies, cell culture media Chemical analysis (trace analysis, HPLC, IC, ICP-MS, TOC measurements) Biosciences (cell and tissue culture media, PCR, DNA, monoclonal antibodies) Flow rate in l/min: up to 2 up to 2 up to 2 up to 2 up to 2 up to 2 Conductivity in µs/cm: Resistivity (MWxcm at 25 C): TOC Value (ppb): Bacterial level (CFU/ml): < 1 < 1 < 1 < 1 < 1 < 1 Particles (0.22 µm/ml): < 1 < 1 < 1 < 1 < 1 < 1 Endotoxines* (EU/ml): RNase* (ng/ml): DNase* (pg/µl): Operating pressure: bar bar bar bar bar bar Mains voltage: 0 V/ 50 Hz 230 V/ 50 Hz 230 V/ 50 Hz 230 V/ 50 Hz 230 V/ 50 Hz 230 V/ 50 Hz Power consumption: 0.1 kw 0.1 kw 0.1 kw 0.1 kw 0.1 kw 0.1 kw Connector size: R ¾ male R ¾ male R ¾ male R ¾ male R ¾ male R ¾ male Ambient temperature: +2 C + 35 C +2 C + 35 C +2 C + 35 C +2 C + 35 C +2 C + 35 C +2 C + 35 C Dimensions W x D x H, in mm: 72 x 330 x x 330 x x 330 x x 330 x x 330 x x 330 x 615 Weight (kg): Cat. no.: GenPure systems TKA GenPure TKA GenPure UF TKA GenPure TKA GenPure incl. xcad dispenser UV-TOC UV-TOC/UF with dispenser with dispenser with dispenser with dispenser bench version bench version bench version bench version Cat. no.: TKA GenPure TKA GenPure UF TKA GenPure TKA GenPure UV-TOC UV-TOC/UF with dispenser with dispenser with dispenser with dispenser wall version wall version wall version wall version Cat. no.: * Depending from the UF = with add. ultrafilter UV-TOC = with UV lamp 185/254 nm photooxidation and TOC display concentration of contaminants UV = with 185/254 nm photooxidation UV-TOC/UF = with UV lamp 185/254 nm photooxidation, TOC display and ultrafiltration in the feedwater Log printer Log printer for connection to purified water and ultra pure water systems with RS 232 interface. For safe documentation of all measured values and faults with date and time in compliance with GLP-Guidelines. Cat. no.: Replacement items and accessories Replacement UV-lamp Ultra pure water cartridge Sterile filter, 0.2 µm Disinfection cartridge Disinfection kit, pack of Volume dosing (standard for GenPure systems with xcad) Qualification documents acc. to GLP TKA xcad External dispensing with control unit Actions radius: up to 0.8 m Tilting radius: 180 Dispensing rate: up to 1.2 l/min Distance from main system: up to 3 m Connectable number of dispenser units: up to 3 10

11 Feedwater requirements for TKA GenPure TKA Pacific. Changes tap water to ASTM Type II water, the ideal feedwater for GenPure. We recommend distilled water or tap water that has been pretreated by flow through a DI type TKA ion exchanger or a TKA Pacific water purification system with connected 30 or 60 litre storage tank. Please request our TKA Pacific UP/ UPW and TKA ion exchange brochures. Feedwater specifications: Colloid index (SDI): max. 1 Conductivity: < 5 µs/cm TOC value: max. 50 ppb Free chlorine: max ppm Turbidity: < 1.0 NTU Carbon dioxide: max. 30 ppm Silicate: max. 2 ppm Pressure: bar Ambient temp.: +2 C +35 C TKA Pacific High Purity Water System Storage tank, 30/60 l GenPure Ultra Pure Water System 11

12 Qualification and Maintenance Technical support in the qualification of your TKA GenPure system The assembly, installation, start-up, operation and maintenance of the system are explained in detail in the Operating Instructions. Proper operation ensures a constant production of ultra pure water within the given specifications for a long time. The Operating Instructions are also an extremely important basis for the qualification of the system. The TKA Qualification Manual This is designed to assist you in qualifying your system. A series of steps lead through Design Qualification (DQ), Installation Qualification (IQ) and Operating Qualification (OQ) to the final evaluation of the qualification documents. Maintenance We recommend that you sign a maintenance contract with your TKA authorized customer service company for the carrying out of all calibration and maintenance work. You then have the assurance that the GLP-conformity and high operational safety and reliability of your system will be maintained. Rights reserved for changes and printing errors. GPxCAD01en Status Go Your TKA authorized specialist distributer: TKA Wasseraufbereitungssysteme GmbH Stockland Niederelbert / Germany Telephone: +49 (0)2602/ Telefax: +49 (0)2602/ info@tka.de

13 18.2 MΩ cm Ultrapure Water. Clean for sure! TKA GenPure. Now also with UV-intensity and TOC monitoring. WATER PURIFICATION SYSTEMS

14 TKA GenPure with UV-intensity and TOC monitoring. Ultrapure water quality - with no reservations! There are areas in which particularly high demands are placed on the water quality. In chemical organic or inorganic trace analysis, for example, for TOC measurements and instrumental methods such as HPLC, ICP-MS and IC. And the demands are even higher in the life sciences. Freedom from endotoxines and nucleases is a necessity when preparing, or working with, tissue and cell culture media, PCR, DNA, or monoclonal antibodies. The Standard, UF, UV and UV/UF versions of GenPure have already convincingly demonstrated the benefits of the new system control and the high-precision, USP-complying conductivity measurement with temperature compensation that can be switched on or off. The new generation GenPure UV-TOC and UV-TOC/UF offer even more: Continuous real-time TOC measurement combined with permanent monitoring of the intensity of the UV-lamp. No longer the risk of false TOC-values with decreasing UV-intensity! The TOC-value shown is correct - you can rely on it! Technical intelligence for reliable processes! User assurance is given by the continual availability of process-related information. The most important measurement results are shown in the actual NONSTOP mode display during the dispensing of ultrapure water. All further displays of values and limiting value settings are on call with the menu-key. Limiting values can be individually set and and protected from unwanted access. A fault message is given should they be exceeded. System controls Digital microprocessor control for fully automatic supervision and control Menu-guided operation with clear text displays of all operating functions and performance parameters Options Choice of English, German or French Conductivity (in µs/cm) or specific electrical resistance (in MW cm) Fault storage Saving of all faults from the last 4 weeks GLP-compliant documentation Real time clock and code-protected operating system prevent unauthorized changes or resetting RS 232 Interface with adjustable send-interval for safe data transfer of all measured data and faults, with date and time, to a PC or log printer Trends or faults can so be timely recognized, and appropriate measures such as cleaning and sanitizing be taken without delay. All automatically at the press of a button, naturally. Further benefits ensure best ultrapure water quality! Automatic interval-switching of the pump for regular recirculation of the ultrapure water through the purification chain and UV-photooxidation. Sanitization of all parts that contact the water for optimal microbiological control!

15 User convenience and GLP-compliance Operating/controlling unit Ergonomic form, can be swivelled for best keypad use and dazzle-free readability IP 54 protection Easy-to-read display Illuminated, 4-line, alphanumeric LCD with 16 characters Simple operation Clearly designed keypad for sure usage and rapid scanning Exact dispensing Finely-controlled PVDF valve allows exact dispensing of the volume required Option of fully automatic volume control over 0.01 to 99.9 Litres for large container filling TKA Measurement methods. Highest accuracy and reliability! n Conductivity measurement The cell constants of the two highprecision conductivity measuring cells are individually measured out and each value deposited in the system. The electronics are automatically calibrated and controlled prior to each measurement. n Temperature measurement Platinum chip temperature sensors with an accuracy of +/- 0.1 C are used here. The temperature compensation can be switched off. USP requirements are fulfilled and optimal measurement reliability ensured. n Online TOC measurement Real time TOC-monitoring with continual and accurate measurement of the organic content of the water within the range 1 99 ppb. n Monitoring of the intensity of the UV-radiation A UV-photodiode permanently checks the intensity of the radiation from the UV-lamp. A decrease in the UV-intensity that would result in a false TOC-value is readily identifiable from the % value shown. The UV-lamp must only be changed when really necessary. UV/TC: Particle-free dispensing Sterile filtration as water is dispensed Protective bell avoids unintentional hand contact of the outlet, e.g. when filling tubes 20 cm clearance offers room for tall receiving vessels n The display on the left shows: Non-Stop: System ready to dispense ultrapure water 1 ppb: Ultrapure water TOC-value µs/cm: Ultrapure water conductivity 23.2 C: Temperature measured for conductivity compensation UV-lamp and temperature compensation switched on (Empty space (Line 4) is reserved for fault messages) n This display shows UV-Time UV-lamp time used h: UV-Intensity Actual UV-intensity in 96%: percent

16 Technical data and accessories Ultrapure water system TKA GenPure UV-TOC TKA GenPure UV-TOC/UF Typical applications: Chemical analysis Life sciences (trace analysis, (cell and tissue culture HPLC, IC, ICP-MS, media, PCR, DNA, TOC-measurements) monoclonal antibodies) Flow rate in l/min: up to 2.0 up to 2.0 Conductivity in µs/cm: Resistance in MW cm at 25 C: TOC-value in ppb: Endotoxines in EU/ml: 0,001 Bacterial content in CFU/ml: < 1 < 1 Particles > 0.22 µm/ml: < 1 < 1 Oper. pressure in bar, min./max.: Electrical requirements: 230 V/ 50 Hz 230 V/ 50 Hz Power consumption: 0.1 kw 0.1 kw Connectors: R 3/4" male thread R 3/4" male thread Ambient temperature: +2 C +35 C +2 C +35 C Serial interface: RS 232 RS 232 Dimensions WxDxH in mm: 372 x 330 x x 330 x 615 Weight in kg: Article No.: TKA GenPure as under-bench version, complete with holder (see photo below). Action radius: up to 0.4 m Angle of swivel: up to 60 Stand height, length: Distance from main unit: acc. to user requirements max. 3 m Replacement materials Pretreatment unit, complete Replacement cartridge for pretreatment unit Replacement UV-lamp Filter cartridge Sterile filter, 0.2 µm Disinfection cartridge Disinfection tablets, pack of 50 Log printer For connection to high purity and ultrapure water purification systems with RS 232 interface. Ensures safe recording of all measured values and faults, complete with date and time, as required by GLP Guidelines. Article no.: Feedwater required: We recommend pretreatment with the TKA ion exchange pretreatment system or UP/UPW Pacific system, or the use of distilled water. Feedwater specifications: Conductivity: < 5 µs/cm Free chlorine: max ppm TOC-value: max. 50 ppb Turbidity: < 1.0 NTU Carbon dioxide: max. 30 ppm Silicate: max. 2 ppm Pressure: bar Ambient temperature.: +2 C +35 C Space saving cupboard installation. The control unit with PVDF dispensing valve can be positioned above for utmost convenience.

17 TKA Technology Ultrapure water from purified water The purified water quality required as feedwater Tap water subjected to ion exchange, reverse osmosis or distillation Optimally, the purified water supplied by a TKA Pacific system The contents of organic and inorganic substances in the feedwater are reduced to values approaching their detection limits by the serial combination of high-efficiency TKA purification technologies described below. GenPure UV-TOC/UF Conductivity: µs/cm Electrical resistance: 18.2 MW cm TOC value: 1 5 ppb Bacterial content: < 1 CFU/ml Particle content: < 1 per ml Endotoxines: EU/ml GenPure UV-TOC: Conductivity: µs/cm Electrical resistance: 18.2 MW cm TOC value: 1 5 ppb Bacterial content: < 1 CFU/ml Particle content: < 1 per ml TKA GenPure UV-TOC/UF Flowchart UV-Photooxidation UV-Photooxidation chamber of a geometry designed for uniform distribution of the 185 nm and 254 nm dualwavelength UV-light. The intensity of the emitted UV-radiation is continually monitored and can be called to display at any time as percentage of the initial intensity. The 254 nm wavelength UV-light kills bacteria by destroying the DNA in their cells, and so effectively minimizes the bacterial content within the system. The combination of the two wavelengths produces very reactive free hydroxyl radicals which oxidize and split small organic substances that are otherwise difficult to remove. The cleavage products are removed in subsequent steps. Adsorption+Ion exchange Purification set containing a combination of highly pure adsorptive material and ion exchange resins. The quality attained here is monitored by continuous TOC and conductivity measurements. The measured values are displayed. This polishing step adsorbs and retains low molecular weight organic molecules, such as UV-photooxidation cleavage products, as well as remaining inorganic ions. Ultrafiltration (Only for GenPure UV-TOC/UF) The asymmetric structure of the polysulfone ultrafilter, and tangential flow across the surface of it, ensure a long service life. Retained substances are led to drain in the concentrate. Ultrafiltration separates suspended and dissolved higher molecular weight contaminants in the water, but serves here mainly to produce water free of endotoxines and nucleases. Membrane filter An 0.2 µm pore size membrane filter in large area capsule form, autoclavable and with venting valve and protective bell. The validated sterilizing filter reliably removes bacteria and particles larger than 0.2 µm directly prior to the ultrapure water outlet. The result: Ultrapure water quality - neat!

18 Qualification and Maintenance TKA Qualification support The Operating Instructions manual describes in detail the procedures that are necessary for preparing, installing, commissioning, operating and maintaining the system for it to consistently produce water within the given quality specifications. This document is also an important basis for the qualification of the system. The TKA Qualification Manual This is designed to assist qualification. The series of steps through Design Qualification (DQ), Installation Qualification (IQ) and Operational Qualification (OQ) leads to a final evaluation of the qualification documentation. Maintenance We recommend that you close a service contract with the TKA service company responsible for your area for carrying out the necessary maintenance and calibration work. You will then have the certainty that your ultrapure water system will be continually kept at a high level of operational safety and readiness, GLP-conform. TKA GenPure without TOC-monitoring Descriptions of TKA ultrapure water systems without TOC monitoring, as well as TKA reverse osmosis systems for laboratory applications are given in the TKA "Ultrapure Water Systems" brochure. Rights to changes and printing errors reserved. GP-TOC01en Status GT Your supplier: TKA Wasseraufbereitungssysteme GmbH Stockland 3 D Niederelbert Telephone: +49 (0) / Telefax: +49 (0) 26 02/ info@tka.de

19 TKA LabTower EDI Two in one! Pure and Ultrapure water WATER PURIFICATION SYSTEMS

20 The integrated system. Purification and tank in one. High purity water ASTM II MΩ cm. For daily needs of 100 to 500 litres. Ready to be called up. Ultrapure water ASTM I 18,2 MΩxcm, TOC 1 5 ppb Direct dispensing of Ultrapure water. Even for the most demanding and sensitive applications. In the Biosciences For molecular biology and microbiology work In the preparation of cell and tissue cultures In PCR, DNA sequencing Electrophoresis In Analytical Chemistry Organic and inorganic trace analysis HPLC GC-MS, ICP-MS, GF-AAS TOC Measurements, IC For example, for Rinsing lab glassware Supplying autoclaves, clinical analysers and ultrapure water systems Preparing and diluting buffers reagents tissue culture media Sample preparation for analytical methods General biotechnology applications Standards The TKA LabTower EDI exceeds the standards ASTM type I and type II, CAP, ISO 3696, BS 3997, CLSI. In Routine Laboratory Work Analytical procedures IC, AAS, ICP-ES

21 TKA LabTower EDI. The benefits in a nutshell! Two in one! Reverse osmosis + EDI + polisher and high purity water tank Two systems, but only one unit One on top of the other instead of alongside Perfectly tuned to each other Formally a unified whole Two ways to draw water! Directly from the system via a dispensing tap with sterile filter (ASTM I) From the tank for direct supplying of autoclaves, analysers, washing machines (ASTM II) Built-in state-of-the-art techniques! Water pretreatment with activated carbon/prefilter and hardness stabiliser Pressure pump for direct supply to autoclaves, analysers, washing machines Optional with: Sterile filter for water dispensed from the tank Sterile vent and overflow UV lamp for tank installation Ultrapure water module additionally with: UV Photooxidation 185/254 nm for the destruction of organic compounds Ultrafilter for removal of endotoxins Certain fresh quality! Directly prior to being dispensed from the system, the ultrapure water is passed through a special polisher module and a sterile filter The high purity water quality is measured immediately prior to the dispensing point Tank Recirculation pump Conductivity measuring cell Polisher Ultrapure water dispensing outlet system Pure water from tank Performance acc. to lab needs! High performance reverse osmosis module combined with electrodeionisation and polisher Three systems, for 15, 30 and 45 litres per hour Compact, stylish, mobile! Takes up no bench space Just ¼ m² floor area suffices Mobile on rollers Safe operation! Microprocessor control for automatic operation Permanent monitoring of all important parameters Automatic return to the operating mode after operating actions Potential-free contact for fault messages Water of constant high quality! Automatic circulation of water in the tank through a special polisher module plus free cycle setting Ensures maintenance of the permanently low conductivity even for long periods of inactivity Assured highest hygiene! Optional UV lamp in the tank recirculation system reduces organic constituents optional sterile filter for tank water outlet

22 Stock high purity water safely and conveniently! 100 Litre tank module! Up to 100 l water on call Sterile vent filter/tank overflow Two safety filters prevent internal contamination by microorganisms from the surrounding air Option: CO 2 absorber that prevents increased TOC value by drawn-in CO 2 Volume display/float chain Fully automatic level regulation for percentage volume display of the tank content Float chain with adjustable volume content Conical bottom drainage Allows complete drainage and so efficient cleaning and disinfection Tank drain High purity water tank outlet Recirculation pump Serves to protect the high purity water from bacterial growth during standstill and safeguards the low conductivity value Polyethylene tank High purity water resistant, opaque and food-safe Designed for Qualification! TKA LabTower EDI has been designed to fulfill GLP requirements. Recording and traceability of data are ensured via an RS 232 interface and a TKA printer. A cell constant of 0.01 cm -1 guarantees highly qualified and precise measurement of the conductivity, which is compensated to an accuracy of ± 0.1 C (acc. to USP <645>) even with only minimal temperature change.

23 Comfortable operation! Clear information! The large 4-line display is code protected and offers a choice of three languages, English, German or French. It is illuminated, easy to read and provides information on: the temperature in C the temperature-compensated conductivity of water coming from the osmosis/high purity polisher module the operating mode status, such as production, stand-by, disinfection the tank content in % the RS 232 interface the fault memory function Easy to work with! Just six control keys allow for rapid operational readiness Start/stop of high purity water production Start/stop of other operating sequencies and service procedures Limiting values settings, conductivity RO water and high purity water Recirculation interval On/off of the optional UV lamp Sterile ultrapure water ASTM I on call! Conductivity measurement directly prior the dispensing point. Protected from re-contamination by a sterile filter. A good view! Just set the tiltable display/keypad unit to the best viewing/operating position for you. Tank level control indicator! Continual monitoring of the tank volume in %.

24 TKA LabTower EDI. Water purification with High-Tech. Combination of electrodialysis and ion exchange processes Electrodeionisation, EDI in brief, unites two proven technologies for producing ultrapure water: Electrodialysis and ion exchange (IEX). The great technical thing about this: In contrast to conventional ion exchange, in which resins must be chemically regenerated, EDI utilizes an electric current for continual resin regeneration. Technologies that keep you one jump ahead! Constant high efficiency of the ion > gives you outstanding ultrapure water exchanger layer bed quality No regeneration chemicals > important for the environmentl No exchange of spent resins > saves you costs High purity water when you need it > no wasted time Purified water < 0,1µS/cm Concentrate Na + OH - Na + CI - Anode (+) Na + CI - H + OH - CI - H + Na + OH - H + Cathode ( ) H + CI - Na + CI - Electrolyte Concentrate Concentrate Electrolyte Concentrate Feedwater Feedwater Cation-permeable membrane Feedwater Permeate from RO system, 4 30 µs/cm Anion-permeable membrane Cation exchanger membrane Anion resin Anion exchanger membrane How EDI works Several layers of ion selective membranes are situated between an anode and a cathode. Layered ion exchanger resin beds and concentrate chambers are alternately positioned between them. negative pole (cathode). The water ions H + and OH that migrate through an ion exchange chamber displace salt ions retained by the ion exchanger resins and so continually regenerate the resins. Concentrate Concentrate Cation resin Diagrammatic representation On applying an electric voltage, water (H 2 0) is split into H + and OH in the cell. The H + and Na + cations can migrate through the cation permeable membranes, anions through the anion permeable membranes. The ions migrate in the direction of the applied voltage, i.e. anions to the positive pole (anode), cations to the The salt ions migrate through the appropriate ion selective membranes into the concentrate chambers and are flushed out of them by water. As all concentration chambers are flushed through one after the other, excess H + and OH ions can again combine to form H 2 0.

25 Flow chart TKA LabTower EDI System components LabTower EDI LabTower type EDI 15 EDI 30 EDI 45 ASTM I conductivity in µs/cm Resistivity (MΩxcm at 25 C) Flow rate up to l/min TOC-Value (ppb) TOC-Value (ppb) with optional UV photooxidation 185/254 nm Bacterial level (CFU/ml) with sterile filter: < 1 < 1 < 1 Particles (> 0.2 µm/ml) with sterile filter: < 1 < 1 < 1 Optional with ultrafilter: Endotoxines* (EU/ml): RNase* (ng/ml): DNase* (pg/µl): ASTM II conductivity in µs/cm** Typical resistivity (MΩxcm at 25 C)** Pure water production at 15 C, l/h Operating pressure, bar, min/max: 2/6 2/6 2/6 Supply voltage: 230/50 230/50 230/50 Power consumption, kw: Connector size: d8 / R 3/4" d8 / R 3/4" d8 / R 3/4" Ambient temperature: + 2 C + 35 C + 2 C + 35 C + 2 C + 35 C Dimensions, W x D x H, mm: 450 x 580 x x 580 x x 580 x 1500 Weight, kg: Cat. no.: * Depending from the concentration of contaminants in the feedwater ** with < 30 ppm CO 2 in the feedwater Accessories Sterile overflow for tank CO 2 Absorber + sterile filter, 0.2 µm, for tank Sterile vent filter, 0.2 µm, for tank UV lamp for tank Activated carbon cartridge with prefilter, 5 µm Hardness stabiliser UV photooxidation 185/254 nm Ultrafiltration capsule Sterile filter, 10", 0.2 µm, for tank pure water outlet Sterile filter, 0.2 µm, for direct dispensing Disinfection kit, pack of Polisher module TKA Feedwater requirements for LabTower EDI systems: Source: Potable water, max µs/cm Prefiltration: 5 µm + activated carbon softener Free chlorine concentration: < 0.1 mg/litre Manganese content: < 0.05 mg/litre Iron content: < 0.05 mg/litre Colloidal index: < 3 ph-range: 4 11 All LabTower EDI systems consist of: Prefilter 5 µm/activated High purity water tank, 100 l carbon cartridge Pressure-/recirculation pump Reverse osmosis with EDI Sterile filter for ultrapure Polisher module water dispensing

26 The purifying systems for laboratory water introduced in this brochure are only a part of the total TKA water purification systems line. TKA Water Purification Systems Among others, TKA offers highest quality reverse osmosis systems with state of the art spiral membranes, both alone and in combination with TKA electrodeionisation. For the smallest to the largest need of high purity water, for the daily supply of water to autoclaves up to the production of Aqua Purificata. We are gladly ready to supply further information, to advise you on the most fitting pretreatment or to recommend the optimal combination of systems for the performance required. Always with economy in mind and under consideration of the water quality you need. As little as possible, as much as necessary! It is our aim to offer you water purification systems that prepare water of various specified qualities as reliably, practically and cost-favourably as possible. Our standard systems are correspondingly designed. Should none of these exactly fit your requirements, however, simply contact us! We see each and every problem as one that needs to be solved and will find a suitable and economic solution even when the conditions are difficult. Rights reserved for changes and printing errors. LT-EDI02en Status Go Your authorised dealer: TKA Wasseraufbereitungssysteme GmbH Stockland Niederelbert / Germany Telephone: +49 (0)26 02/ Telefax: +49 (0)26 02/ info@tka.de

27 TKA LabTower UP/UPW The High-Tech complete system. High purity water for a wide range of laboratory and medical applications. WATER PURIFICATION SYSTEMS

28 The integrated system. Purification and storage in one. This High-Tech complete system has been specifically designed for laboratory and medical areas The high purity water quality produced fulfills all requirements on rinse water for medical use acc. to pr EN15883, DIN EN 285/13060 standards... For supplying cleaning and disinfecting equipment for surgical instruments, vessels and glassware as well as anesthetic equipment For supplying autoclaves and steam sterilizers...and on laboratory water acc. to ASTM II, CAP, ISO 3696 standards For feeding ultra pure water systems For glassware rinsing For preparing and diluting buffers, reagents For preparing samples for analytical methods For general biotechnological applications

29 TKA LabTower UP/UPW. The benefits in a nutshell! Excels as a laboratory supplier! Combination of a high performance reverse osmosis module with longlife ion exchanger Two systems, for either 20 or 40 litres of high purity water per hour Compact, attractive design, movable! Needs no bench-top space A mere ¼ m² floor space suffices Castors allow easy re-location Dependable operation! Microprocessor control directs automatic operation and monitors all important parameters Potential-free contact allows fault message evaluation Leak monitoring via aquastop water control with solenoid valve (optional) Water quality always fresh! Accurate water quality data are assured via the built-in RS 232 interface and printed out Purification and quality measurement immediately before high purity water is drawn off Two in one! Reverse osmosis water purification together with high purity water tank Two systems in a single whole Two ways to dispense water! Directly from the system via the dispensing valve for calibration solution preparation, manual analyses, filling of containers, general rinsing purposes, etc. From the tank for direct supply to autoclaves, washing machines, ultra pure water systems Built-in state-of-the-art technology! Water pretreatment with 5 µm prefilter, activated carbon and hardness stabilization 1 µm Particle removing filter at the tank outlet of high purity water Optionally with: Sterile filter unit for the tank water outlet Sterile vent filter/overflow UV Immersion lamp for installation in the tank Flow through UV sanitization of high purity water (UPW version only) Greater economy! Low running costs are ensured by the stainless steel cartridge containing a 20 l stock of ion exchange resins. These TOC tested resins can be simply replaced by disposable resin and/or be regenerated

30 Store hig purity water safely and conveniently! 100 Litre tank module! Up to 100 litres of high purity water ready for dispensing when needed Conical bottom outlet Allows complete emptying for efficient cleaning and disinfection Sterile vent filter/tank overflow (optional) These two protective filters prevent airborne contamination by microorganisms Volume display/float chain Completely automatic level regulation with display of the currently stored volume in the tank Float chain for adjustable tank volume Tank drain Tank bottom outlet high purity water Polyethylene tank High purity water resistant, opaque to light and food-safe Comfortable access via the cleaning opening Documentation TKA LabTower UP/UPW has been developed to fulfill GLP (Good Laboratory Practice) requirements. Recorded and traceable data can be obtained by print out via the RS 232 interface and a TKA printer. Highly qualified and precise measurement of the conductivity is ensured by the cell constant of 0.01 cm -1 and the compensation of it, even with minimal changes in temperature, to an accuracy of ± 0.1 C (acc. to USP <645>).

31 Optimal user comfort Clear information! The large 4-line display is code-protected and offers a choice of 3 languages; English, German or French. It is illuminated, easily readable and provides information on: The temperature in C The temperature compensated conductivity of the water subsequent to the reverse osmosis/high purity water module the operating mode status, such as production, stand-by, sanitization the tank content in % the RS 232 interface the fault memory function Easy to work with! Just six control keys allow for rapid operational readiness Start/stop of high purity water production Start/stop of other operating processes and routine maintenance Setting the limiting conductivity value for reverse osmosis water and high purity water On/off of the optional UV-lamp Sterile high purity water on call! Freshly produced high purity water is always there to be taken from the tank or directly from the system. Protected from re-contamination by an optional sterile filter. A good view! Just set the tiltable display/keypad unit to the best viewing/operating position for you. Tank level control indicator! Continual monitoring of the tank volume in %.

32 Flow chart for TKA LabTower UP/UPW LabTower UP/UPW. High-Tech purification to pure water. Pretreatment Reverse osmosis and tank Ion exchanger/uv Dispensing from the front The 5 µm prefilter and activated carbon in the combi-filter cartridge protect downstream purification stages from free chlorine and particles. The subsequent hardness stabilizing cartridge protects the reverse osmosis stage from hardness forming substances. TKA can offer further pretreatment possibilities for unfavourable feedwater conditions on request. The pretreated water is pressure-forced through the permeable reverse osmosis module, whereby % of all inorganic ions and 99 % of all dissolved organic substances, as well as microorganisms and particles, are removed from the water. The built-in 100 l tank has a conical bottom outlet and a suitable lidded opening for easy and efficient cleaning and sanitization. The permeate that emerges from the reverse osmosis module has been purified to 99 %. The very small amounts of cations and anions that are still in the water are now completely removed by passage of it through the TOC tested, high-purity resins in the ion exchange cartridge. Irradiation with UV light (UPW version only) eliminates any bacteria or germs that are possibly still present. A 1 µm particle removing filter finally brings the optimal result: Water in its purest form. An additional dispenser at the front of the system itself offers a very convenient dispensing possibility. Flow rate, 1.5 l/min. Optionally with an 0.2 µm sterile filter The result: H 2 O pure nothing else! Pure water of type ASTM II that also complies with pr EN15883, DIN EN 285 and DIN standards Direct feeding in conformity with the specified standards! A built-in booster and distribution pump ensures the low-noise purified water supply to downstream units. With a performance of 3 l/min the pump provides both the complete laboratory high purity water supply, e.g. for washing machines and ultrapure water systems, and also the direct feeding to cleaning and disinfection equipment and autoclaves.

33 System components, LabTower UP/UPW Type LabTower UP/UPW 20 UP/UPW 40 Flow rate at 15 C, l/h: Typical conductivity in µs/cm: Typical resistance in MΩxcm: 10 1 Bacterial content in CFU/ml, with sterile filter: < 1 Particle content (0.2 µm) per ml, with sterile filter: < 1 Operating pressure in bar, min./max.: 2/6 Supply voltage: 230 V/ 50Hz Power consumption in kw: 0.25 Connector size, male threads: R 3/4 Ambient temperature: +2 C +35 C Dimensions, W x D x H in mm: 450 x 580 x 1500 Weight in kg: Cat. no. UP, 3 l/min pressure pump: Cat. no. UPW*, 3 l/min pressure pump: * UPW-Version with flow through UV sanitization TKA feedwater requirements for LabTower UP/UPW systems: Type of water: Tap water, max ppb Prefiltration: 5 µm + activated carbon Free chlorine concentration: < 0.1 mg/litre Manganese content: < 0.05 mg/litre Iron content: < 0.05 mg/litre Colloid index: < 3 ph-range: 4 11 Accessories Sterile overflow Sterile vent filter, 0.2 µm UV Lamp for the tank UV Lamp for flow-through sanitization prefilter 10", poresize 0.1 µm for tank pure water outlet Sterile filter capsule, 0.2 µm for direct dispensing Leak monitoring with aquastop control and solenoid valve RDS Ion exchanger, 20 l, replacement cartridge Replacement ion exchanger resin for cartridge RDS, 2 x 22 liters Replacement disposables Activated carbon cartridge with prefilter 5 µm, for pretreatment Hardnes stabilizer x reverse osmosis membrane for LabTower 20 l/h x reverse osmosis membrane for LabTower 40 l/h Replacement UV immersion lamp for tank Replacement UV lamp for UP/UPW systems Sanitization kit, pack of 12

34 The purification systems for providing laboratory water that are described in this brochure are only part of the comprehensive TKA water purification systems line. TKA Water purification systems Among others, TKA offers high quality reverse osmosis systems containing state-of-the-art spiral wound membranes, alone or in combination with TKA ion exchangers and electrodeionization modules. Systems for the smallest to the largest need of purified water, for the daily supply of autoclaves up to the production of Aqua Purificata. We will gladly provide you with further information, advise you on the best choice of pretreatment or recommend an optimal combination of various systems for the performance you require. Always with economy in mind and under consideration of the water quality or qualities that you need. As little as possible, as much as necessary! It is our aim to offer you water purification systems that provide water of the various qualities as reliably, practically and cost-favourably as possible. Our standard systems are correspondingly designed. Should none of these exactly fit your specific requirements, however, simply contact us! For us, each and every problem is one to be solved. We will find a suitable and economic solution even when conditions are very unfavourable Rights to changes and printing errors reserved. LT-UPUPW01en Status x000 GT Your authorized dealer: TKA Wasseraufbereitungssysteme GmbH Stockland 3 D Niederelbert Telephone: +49 (0) Fax: +49 (0) info@tka.de

35 Ultrapure water for the daily needs. Easy and economical! TKA MicroLab. ASTM Type II water. Instantly and fresh. WATER PURIFICATION SYSTEMS

36 TKA MicroLab. All that you need. ASTM Type II Ultrapure water. For example, for Rinsing laboratory glassware Preparing and diluting buffers, reagents, tissue culture media and dyes Sample preparation for analytical methods such as flame AAS Instantly and fresh. With TKA MicroLab. This is the password for converting normal tap water to ASTM Type II ultrapure water. All in one! TKA Microlab is a compact complete-system that produces 6 litres of ultrapure water per hour. The built-in 6 litre tank for ultrapure water has a conical bottom for complete emptying and is equipped with a sterile vent filter and a pressure pump for supplying equipment connected downstream (e.g. autoclaves, analyzers). If required, a flexible dispenser with a sterile filter can be connected to the tank. The system has a small footprint, is very attractively designed and looks good in any laboratory. TKA MicroLab operates with the same high-tech purification procedures as the well known TKA Pacific systems. Outstanding design! TKA MicroLab Not only the elegant exterior advances laboratories into the future!

37 TKA MicroLab. Ultrapure water at the press of a button! Position it, connect it, use it! Economical! Two long-life cartridges. Each requires replacement only when really necessary! One, two, three rapid cartridge change due to quickconnects. Space saving! Simply stand it on the laboratory bench, or mount it directly to the wall. Convenient! Swivel-mounted display - always easy to read UV-lamp! Option of a UV-lamp for microorganism reduction.

38 TKA MicroLab Flow chart. The result: Ultrapure water of the required quality: ASTM Type II 6 Litres per hour. Always freshly produced and ready to be taken at any time - either from the built-in 6 litre tank or via the separate dispensing valve with sterile filter. Conductivity in µs/cm: Resistance in MW cm at 25 C: Bacterial content in CFU/ml: < 1 Particle content: < 1 Permeate performance per hour: Flow rate per minute: 6 litres 1.0 litre Absolutely sterile! Ultrapure water is dispensed through a standardly supplied sterile filter.

39 More ideas = more benefits How TKA MicroLab differs from other compact-class systems: Powerful performance! Permeate flow 6 l/h Flow from sterile filter outlet 1.0 l/min 6-Litre tank with integrated pressure and recirculation pumps Lasting economy! Water purification progresses through separate modules, each with quick connects for simple individual replacement Module 1 Pretreatment module with integrated reverse osmosis membrane Module 2 Cartridge containing high-quality ultrapure resins with very long service intervals Easiest to operate! Just press a button to get ultrapure water - no adjustments necessary, the microprocessor control controls itself Ultrapure water quality is ensured by the limiting value input RS 232 Interface The display can be tilted to your optimal reading position Placement anywhere Stand it on the laboratory bench Mount it on the wall Usable worldwide! Whether 220/230 V, 110/120 V or other voltages - and even with fluctuating voltages - the automatic voltage regulator continually supplies the system with a constant 24 volts Almost maintenance-free! The tank is made of ultrapure water resistant, pigment-free and food-safe polyethylene and has a conical bottom outlet for complete emptying Can be easily cleaned and disinfected

40 Specifications TKA MicroLab Permeate performance at 15 C: 6 l/h Flow rate: 1.0 l/min Operating pressure in bar, min/max: 1 6 bar Conductivity: µs/cm Resistance at 25 C: MΩ cm TOC Value: - Bacterial content in CFU/ml: < 1 Particle content: < 1 Supply voltage: Automatic voltage regulation to 24 V Power consumption: 0.06 kw Connector size: R 3/4" Dimensions, W x D x H in mm: 305 x 400 x 545 Weight: 22 kg Article no.: * with UV-lamp Consumables and accessories Wall-mount Article no Pretreatment module with RO-membrane Article no Ultrapure water cartridge Article no Sterile filter Article no Vent filter for tank Article no TKA MicroLab UV 6 l/h 1.0 l/min 1 6 bar µs/cm MΩ cm < 30 ppb < 1 < 1 Automatic voltage regulation to 24 V 0.06 kw R 3/4" 305 x 400 x kg * Rights reserved for changes and printing errors. Printed in EU Status GT Your TKA Representative: TKA Wasseraufbereitungssysteme GmbH Stockland 3 D Niederelbert Telephone: +49 (0) / Telefax: +49 (0) / info@tka.de

41 High purity water for the lab's daily needs. It s so easy! TKA MicroMed. Purer. Quicker. Greater economy. WATER PURIFICATION SYSTEMS

42 TKA MicroMed. Purer. Quicker. Greater economy. High purity water, better than ASTM Type III. For daily needs in the lab: Numerous analytical requirements Standard chemical applications Reagent preparation Autoclave operation Thermodisinfectors Ultrasonic cleaning Instrument cleaning Swivel-mounted display - always easy to read Instantly and fresh. With TKA MicroMed. This is the password for converting normal tap water to ASTM Type III high purity water. All in one! TKA MicroMed is a compact system that produces 6 litres per hour. The built-in 6 litre tank for high purity water has a conical bottom for complete emptying and is equipped with a sterile vent filter (optional) and a pressure pump for supplying equip- TKA MicroMed Not only the elegant exterior advances laboratories into the future! ment connected downstream (e.g. autoclaves, analyzers). If required, a flexible dispenser with a sterile filter can be connected to the tank. The system has a small footprint, is very attractively designed and looks good in any laboratory. TKA MicroMed operates with the same high-tech purification procedures as the well known TKA Pacific systems.

43 TKA MicroMed Flow chart. The result: High purity water better than ASTM Type III. Conductivity in µs/cm: Resistance in MW cm at 25 C: 10 1 Percentage membrane retention of germs, bacteria, particles: 99% Flow rate per minute: Permeate performance per hour: 1.0 litre 6 litres High purity water at the press of a button! Position it, connect it, use it! Space saving! Simply stand it on the laboratory bench, or mount it directly to the wall. Economical! Two long-life cartridges with quickconnects. Each requires replacement only when really necessary!

44 More ideas = more benefits! How TKA MicroMed differs from other compact-class systems: Powerful performance! Flow rate per minute, 1.0 l 6-Litre tank with integrated pressure and recirculation pumps Lasting economy! Water purification progresses through separate modules, each with quick connects for simple individual replacement Module 1 Pretreatment module with integrated reverse osmosis membrane Module 2 Cartridge containing high-quality resins with very long service intervals Easiest to operate! Just press a button to get high purity water - no adjustments necessary, the microprocessor control controls itself High purity water quality is ensured by the limiting value input RS 232 Interface The display can be tilted to your optimal reading position Placement anywhere Stand it on the laboratory bench Mount it on the wall Usable worldwide! Whether 220/230 V, 110/120 V or other voltages - and even with fluctuating voltages - the automatic voltage regulator continually supplies the system with a constant 24 volts Almost maintenance-free! The tank is made of ultrapure water resistant, pigment-free and foodsafe polyethylene and has a conical bottom outlet for complete emptying Can be easily cleaned and disinfected

45 Distillation was yesterday. It is true that water has been heating to boiling and relatively pure water won by condensing the steam for hundreds of years, but this traditional method is complicated, time-consuming and also nowadays very expensive. Costly in electricity 1 2 kw of energy are needed for 2 l/h of distilled water Large cooling water requirement Litres of cooling water are needed for 2 litres of distilled water Lots of room A water still needs quite some space High cleaning expenditure Particularly with hard water Uncertain quality No monitoring, no display Great risk of contamination Distilled water is held in closed containers. Each opening of the closure can lead to the ingress of contamination and reduced quality Low flow rates Small laboratory stills only produce 2 l/h of distilled water. It must therefore be produced for stock It's time to change!

46 Specifications and accessories TKA MicroMed Permeate performance at 15 C: 6 l/h Flow rate: 1.0 l/min Operating pressure in bar, min/max: 1 6 bar Conductivity: µs/cm Resistance at 25 C: 10 1 MW cm Percentage of germs, bacteria, particles retained by the membrane: 99 % Supply voltage: Automatic voltage regulation to 24 V Power consumption: 0.06 kw Connector size: R 3/4" Dimensions, W x D x H in mm: 305 x 400 x 545 Weight: 22 kg Cat. no.: Consumables and accessories Wall-mount Cat. no Pretreatment module with RO-membrane Cat. no Ion exchange cartridge Cat. no Vent filter for tank Cat. no Rights reserved for changes and printing errors. Printed in EU Status Go Your TKA Representative: TKA Wasseraufbereitungssysteme GmbH Stockland Niederelbert / Germany Telephone: +49 (0) / Telefax: +49 (0) / info@tka.de

47 18.2 MΩ cm Ultrapure Water. Fresh on Demand! TKA MicroPure. New! For small volumes umpteen times a day! WATER PURIFICATION SYSTEMS

48 TKA MicroPure. Favourable price. Compact. Reliable. The Micro-sized Ultrapure Water System! Everything that most laboratories need! Many laboratories require ultrapure water every day - for HPLC or for other instrumental analyses, for sample preparation, for the rinsing of glassware or for molecular biology work in which endotoxines and/or nucleases would interfere - but only as small volumes at a time, up to a total of a few liters at the most. However, this is no reason to do without professional, freshly-prepared ultrapure water and buy expensive HPLC water. After all, with the repeated taking of small volumes from an HPLC water container, the quality of the water and the TOC-value no longer fulfill the specifications. The ultrapure water is no longer ultrapure! 18.2 MΩ cm quality! The designation ultrapure water is only normally used for water that fulfills the following specifications: Resistance: 18.2 MΩ cm TOC value: max. 10 ppb Bacterial content: < 1 CFU/ml Particles > 0.22 µm: < 1/ml TKA MicroPure gives you more: Ultrapure water as you need! Always fresh. Always available in the volume required. Always with the required quality. And always for only a few cents per liter! Water to suit the job professionally, from one of four different versions, according to requirements. Typical Instrumental High-sensitivity Molecular biology, Microbiology, applications analytical methods instrumental PCR, DNA, cell molecular biology, such as AAS, IC, analytical methods, cultures, mono- PCR, monoclonal ICP such as HPLC, ICP-MS, clonal antibodies antibodies, TOC-analyses, MC cell culture media Product water Flow rate, L/min: characteristics Conductivity, µs/cm: Resistance at 25 C, MΩ cm: TOC value, ppb: Endotoxines, EU/ml: 0.001* 0.001* Bacterial content, CFU/ml: < 1 < 1 < 1 < 1 Particles > 0.2 µm, per ml: < 1 < 1 < 1 < 1 Type MicroPure MicroPure UV MicroPure UV/UF MicroPure UF Article no * Dependent on feedwater and sanitization

49 Flow chart TKA MicroPure UV/UF. ON/OFF Switches the system ON or OFF DISPLAY Illuminated clear text display NONSTOP STARTS ultrapure water production UV Switches the UV lamp ON or OFF INTERVAL Brings the system to STAND BY mode In stand by mode and in production mode, the display permanently shows the ultrapure water CONDUCTIVITY and TEMPERATURE. MENUE To call and alter LIMITING VALUES for CLEANING and DISINFECTION Designed for simplest operation. The central control display makes all functions conveniently available, so that TKA MicroPure is ready to operate at the press of a button. Just switch it on. Everything else is automatically managed by the built-in microprocessor system control. The interval switching of the recirculation pump for prevention of bacterial growth during standstill, for example, as well as permanent monitoring of all limiting values and the immediate display of fault messages. One, two, three rapid cartridge change due to quickconnects. Dependable ultrapure water dispensing! A protective bell on the sterile filter outlet prevents accidental contamination from finger contact. Optional feedwater tank! The MicroPure is also available on request with a 6 liter feedwater tank for filling with distilled water.

50 Technical details and accessories TKA MicroPure Electrical requirements: 230 V / Hz Power consumption: 0.06 kw Operating pressure, min/max: bar Ambient temperature: min. 2 C, max. 35 C Dimensions, W x D x H: 305 x 300 x 545 mm Weight: approx. 17 kg Feedwater connector: Male R 3/4 Installation: Bench-top, optional wall mounted Consumables and accessories Sterile filter, 0.2 µm Article-No Filter cartridge, Micro Article-No Pretreatment system, complete Article-No Replacement cartridge for pretreatment system Article-No Replacment UV lamp Article-No Disinfection cartridge Article-No Disinfection tablets, pack of 12 Article-No Wall mounting unit for MicroPure Article-No TKA MicroPure type Ultra pure water system MicroPure Article-No Ultra pure water system MicroPure with ultrafiltration Article-No Ultra pure water system MicroPure with ultrafiltration and UV photo-oxidation 185/254 nm Article-No Ultra pure water system MicroPure with UV photo-oxidation 185/254 nm Article-No Feedwater pretreatment: We recommend pretreatment by means of a TKA pretreatment system or Pacific UP/UPW system, or distillation. Feedwater specifications: Silt density index (SDI): max. 1 Conductivity: < 5 µs/cm Free chlorine: max ppm TOC value: max. 50 ppb Turbidity: < 1.0 NTU Carbon dioxide: max. 30 ppm Silicate: max. 2 ppm Pressure: bar Ambient temp.: +2 C +35 C Rights for changes and printing errors reserved MPure01engl Status Go The MicroPure systems above are also available with an optional 6 liter feedwater tank, e.g.: MicroPure + tank Article-No ST Your dealer: TKA Wasseraufbereitungssysteme GmbH Stockland Niederelbert / Germany Telephone: +49 (0)2602 / Telefax: +49 (0)2602 / info@tka.de

51 From tapwater to high purity water fulfilling ASTM II, CLSI, CAP, ISO 3696 water standards TKA Pacific UP/UPW High purity water systems for every laboratory WATER PURIFICATION SYSTEMS

52 Mount, connect, start! TKA Pacific. Converts tapwater into MΩ cm* high purity water. Water of the type that is needed daily for typical laboratory applications: Rinse water for laboratory glassware Feedwater for autoclaves, clinical analyzers and ultrapure water systems Preparation and dilution of buffers, reagents, tissue culture media and dyes Sample preparation for analytical methods such as flame AAS * better than ASTM Type II

53 TKA Pacific. Attractive, practical, reliable. Attractive design! Elegance outside, High-Tech inside. The ultimate in systems for the automatic and economical production of high purity water for daily requirements of from 20 to 200 litres. Operational reliability! Microprocessor controlled for automatic operation with permanent monitoring of all important parameters. Automatic return to the operating mode when operating processes end. Potential free contact for fault messages. Excellent performance! Option of a UV lamp for the elimination of even the finest biological contaminants. Economical to run! In contrast to the older method of distillation that requires much energy and cooling water, TKA Pacific requires only 0.1 kw/h for the operation of the pump. Space-saving positioning! TKA Pacific systems can be stood on a lab bench or be fitted to a wall without an additional mounting bracket. Qualifiable! Pacific systems have been developed to comply with GLP (Good Laboratory Practice) requirements. A TKA printer connected to the RS 232 interface allows print-outs for data recording and traceability to be triggered at any time. A cell constant of 0.01 cm -1 ensures highly qualified and precise conductivity measurements, which are compensated to an accuracy of ± 0.1 C for even minimal temperature changes (in conformity with USP <645>).

54 TKA Pacific. Comfortable to use! Flexible control and operating unit. The display/keypad unit can be tilted and can so always be brought to the optimal position for viewing and keyoperation. Clear information! The large, illuminated 4-line display is easy to read and provides information on: The temperaure in C The temperature compensated conductivity of the water coming from the reverse osmosis/high purity water cartridge The UV lamp operating hours (UPW version only) The operating mode status, such as production, stand-by, cleaning, disinfection The tank content in % The RS 232 interface Fault message storage Code protected 3 languages (D; E; F) Easy to use! Six keys only for rapid operational readiness: Start/stop high purity water production Start/stop other operational sequencies or service procedures Setting of limiting conductivity values for RO-water and high purity water Setting of the circulation interval Start/stop UV lamp (UPW version only)

55 Flow chart TKA Pacific UPW. TKA Pacific with high purity water storage tank. A complete system. Pretreatment: Two purification steps are carried out to protect the reverse osmosis membrane. The hardness stabilizing cartridge prevents the precipitation of hardness-formers on the membrane. The second step removes free chlorine and particles. Reverse osmosis membrane: The high performance reverse osmosis membrane removes approx. 98 % of inorganic ions and 99 % of all dissolved organic substances, microorganisms and particles. High purity water cartridge: This cartridge further purifies the reverse osmosis permeate to produce high-purity water of the µs/cm quality that is required by international standards (ASTM II, CAP, ISO 3696, BS 3997 and CLSI, previously NCCLS, Type 1). UV Lamp (UPW version only): The 254 nm UV transmission ensures optimal UV irradiation. Light of wavelength 254 nm acts germicidal. Recirculation: Recirculation of the water between the storage tank and Pacific UPW is an important improvement for maintenance of the water quality. The recirculation path is from the storage tank is through the high purity water cartridge and the UV chamber, then back to the tank. Tap water pretreatment and multi-stage purification Potable tap water Inlet hose, raw water 4 Pretreatment unit ( ) Connecting hose to TKA Pacific TKA Pacific high purity water system 3/7/12/20/40 l/h Connection, Pacific to storage tank Storage tank, 30 l ( or 5033), 60 l ( or 5063), 100 l ( or 5083) Drain

56 TKA Standard storage tanks. For 30, 60 or 100 litres. Store high purity water safely and conveniently! An advanced and proven tank construction with many beneficial features: Large opening For easy and effective cleaning by hand. Closed with a screw cap. Sterile venting filter/tank overflow Contamination by microorganisms in the environment is prevented by two safety filters. Option: A CO 2 absorber that prevents TOC value increases from drawn-in CO 2. Float switch/volume display Completely automatic water level regulation, with analog signal to show the percentage volume in the tank in the Pacific display. Polyethylene tank Ultrapure water resistant, opaque to light and food-safe. High purity water dispensing tap With 24 cm free space for practical water removal Recirculation pump/pressure pump The recirculation pump protects the high purity water from bacterial growth during standstills and maintains the low conductivity value The pressure pump can pump high purity water to laboratory autoclaves and clinical analyzers Wall mount For simple and space-saving wall mounting Conical bottom outlet Allows complete draining and efficient cleaning and disinfection

57 Pacific UP/UPW system components and accessories Type Pacific UP/UPW Pacific 3 Pacific 7 Pacific 12 Pacific 20 Pacific 40 Flow rate at 15 C, l/h: Typical conductivity UP*/UPW* (µs/cm) ,10 Resistance at 25 C UP*/UPW* (MW cm) Retention quota bacteria, %: 99 Silicate removal, %: > 99.9 TOC Value, ppb: < 30 Operating pressure in bar, min./max.: 2/6 Supply voltage: Power consumption, kw: 0,1 230 V/50 Hz Connector size, male thread: R 3/4 Ambient temperature: +2 C +35 C Dimensions, W x D x H, mm: 372 x 330 x 613 Weight in kg, Standard/with UV: 24/25 24/25 25/26 25/26 25/26 Cat. no., UP version: Cat. no., UPW** version: * With < 30 ppm CO 2 in the feedwater ** Version with UV lamp, bacteria content < 1 CFU/ml TKA Requirements for feedwater for Pacific systems: Feedwater: Free chlorine concentration: Manganese content: Iron content: Colloid index: < 3 PH range: 4 11 Potable water or maximum conductivity 1500 µs/cm < 0.01 mg/liter < 0.05 mg/liter < 0.05 mg/liter Pre-treatment for TKA Pacific UP/UPW: Cat. No Complete pre-treatment consisting of: 2 x Filter housing 10" with activated carbon with prefilter 5 µm and hardness stabilizer to remove particles, free chlorine and hardness formers to protect the RO-membrane. System expendables and accessories for TKA Pacific: Cat. No Activated carbon cartridge with 5 µm prefilter Cat. No Hardness stabilizing cartridge Cat. No Disinfection kit, pack of 12 Cat. No UV Lamp (UPW version only) Cat. No High purity water cartridge Cat. No Reverse osmosis module

58 Storage tanks and accessories Standard storage tanks Volume: Material: Outlet tap: Overflow: Diameter: 30 litres, 60 litres or 100 litres PE acc. to ME 8159 and NG2431 E, ultrapure water resistant, opaque to light, food-safe 240 mm free headroom d8 hose connector 388 mm Height, 30/60/100: 606 mm / 920 mm / 1259 mm Weight in kg: Tank 30/60/100 6 /7/9 (Standard) Tank 30 / 60/100 8 /10/12 (with recirculation/ pressure pump) Tank, 30 liter volume Cat. No Cat. No litre storage tank with level display and recirculation/pressure pump 30 litre storage tank with level display Tank, 60 liter volume Cat. No Cat. No litre storage tank with level display and recirculation/pressure pump 60 litre storage tank with level display Tank, 100 liter volume Cat. No Cat. No litre storage tank with level display and recirculation/pressure pump 100 litre storage tank with level display Complementary accessories Cat. No Sterile overflow Cat. No CO 2 Absorber + sterile venting filter, 0.2 µm Cat. No Sterile venting filter, 0.2 µm Cat. No UV Immersion lamp for 30 l tank, 60 l tank and 100 l tank Cat. No Wall mounting bracket for 30 l storage tank Cat. No Wall mounting bracket for 60 l storage tank

59 For quality and economy without compromise! When the water purity must fulfill even higher demands! TKA Pacific Storage tank TKA GenPure Then 18.2 MΩ cm ultrapure water. TOC value 1 10 ppb. Prepared by the TKA GenPure ultrapure water system. Simply install it downstream of the Pacific system. TKA GenPure UF With ultrafiltration for the certain removal of pyrogens and nucleases. TKA GenPure UV With UV photooxidation for further reduction of organic contaminants. TKA GenPure UV/UF With a combination of ultrafiltration and UV photooxidation. TKA GenPure UV/TOC and UV/TOC/UF With UV photooxidation and display of the TOC value. Validatable and qualifiable in compliance with GLP. Optionally also with ultrafiltration. Peak technology for the highest demands and smaller volumes. Please request our TKA GenPure brochure!

60 The purification systems for laboratory water described in this brochure are only a part of the complete TKA line of water purification systems. TKA Water purification systems For example, TKA also offers top quality reverse osmosis systems with state of the art spiral membranes, alone or in combination with TKA electrodeionization. Systems for the smallest to the largest need of high purity water, from the daily supply of water to autoclaves up to the production of Aqua Purificata. We gladly supply further information, advise you on the most fitting pretreatment and recommend the optimal combination of different systems for the performance required. Always with economy in mind and under consideration of the water quality you need. As little as possible, as much as necessary! It is our aim to offer you water purification systems that prepare water of the various qualities as reliably, practically and cost-favourably as possible. Our standard systems are correpondingly designed. Should none of these exactly fit your requirements, however, simply contact us! We see each and every problem as one that is worth solving and will find a suitable and economic solution even when the conditions are difficult. Rights reserved for changes and printing errors. Pac02en Status Go Your dealer: TKA Wasseraufbereitungssysteme GmbH Stockland Niederelbert / Germany Telephone: +49 (0) 2602/ Telefax: +49 (0) 2602/ info@tka.de

61 Everyday ultrapure water. Even for demanding lab applications. Compact high-tech! TKA Smart2Pure. Two in one. ASTM Types I/II water. Instantly and fresh. WATER PURIFICATION SYSTEMS

62 TKA Smart2Pure. For small volumes. But of highest quality! ASTM Type I Ultrapure water. For example, for: Molecular biology, microbiology, PCR, inorganic and organic trace analysis HPLC, ICP TOC-analysis ASTM Type II Ultrapure water. For example, for Rinsing laboratory glassware Preparing and diluting buffers, reagents, tissue culture media and dyes Sample preparation for analytical methods such as flame AAS Instantly and fresh. With TKA Smart2Pure Convenient Swivel-mounted display - always easy to read! This is the password for converting normal tap water to ultrapure water of ASTM Types I and II. All in one! TKA Smart2Pure is a compact complete system that produces 3 or 6 litres of ultrapure water per hour. The built-in 6 litre tank for ultrapure water has a conical bottom for complete emptying. It is equipped with a sterile vent filter and a pressure pump for supplying equipment connected downstream (e.g. autoclaves, analyzers). If required, a flexible dispenser with a sterile filter can be connected to the tank. The system has a small footprint, is very attractively designed and looks good in any laboratory. TKA Smart2Pure operates with the same high-tech purification procedures as the well known TKA Pacific and GenPure systems.

63 TKA Smart2Pure. Ultrapure water at the press of a button! Position it, connect it, use it! Economical! Two long-life cartridges. Each requires replacement only when really necessary! One, two, three rapid cartridge change due to quickconnects. Space saving! Simply stand it on the lab bench, or mount it directly to the wall. High performance filter! Option of an integrated ultrafilter, generously dimensioned for pyrogene-free and nuclease-free water. The automatic self-cleaning gives years of service. UV Oxidation 185/254 nm! Optionally with additional UV oxidation for reducing the content of microorganisms and their metabolites.

64 TKA Smart2Pure UV/UF Flow chart. The result: Ultrapure water of the quality required: ASTM Type I Production of ultrapure water for low volume requirements. The ultrapure water is delivered via a sterile filter. Conductivity in µs/cm: Resistance in MW cm at 25 C: 18.2 Bacterial content in CFU/ml: < 1 Particle content: < 1 Flow rate per minute: 1.0 litre ASTM Type II 3 or 6 Litres per hour. Always freshly produced and ready-to-take at any time from the built-in 6 litre tank. Conductivity in µs/cm: Resistance in MW cm at 25 C: Percentage of germs, bacteria, particles retained by the membrane: 99 % Flow rate per minute: 1.0 litre Absolutely sterile! Ultrapure water is dispensed through a standardly built-in sterile filter.

65 More ideas = more benefits! How TKA Smart2Pure differs from other compact class systems: Powerful performance! Permeate flow 3 or 6 l/h Flow rate 1.0 l/min 6-Litre tank with integrated pressure and recirculation pumps Lasting economy! Water purification progresses through separate modules, each with quick connects for simple individual replacement Module 1 Pretreatment module with integrated reverse osmosis membrane Module 2 Cartridge containing high-quality ultrapure resins with very long service intervals Easiest to operate! Just press a button to get ultrapure water - no adjustments necessary. The microprocessor control is a self-controlling component Ultrapure water quality is ensured by the limiting value input RS 232 Interface The display can be tilted to your optimal reading position Positioning anywhere in the lab Laboratory bench-top operated or wall-mounted Usable worldwide! Whether 220/230 V, 110/120 V or other voltages - and even with fluctuating voltages - the automatic voltage regulator continually supplies the system with a constant 24 volts Almost maintenance-free! The tank is made of ultrapure water resistant, pigment-free and food-safe polyethylene and has a conical bottom outlet for complete emptying Can be easily cleaned and disinfected

66 Technical specifications TKA Smart2Pure Standard TKA Smart2Pure UF* Permeate performance at 15 C: 3 l/h or 6 l/h 3 l/h or 6 l/h Flow rate: 1.0 l/min 0.6 l/min Operating pressure in bar, min/max: 1 6 bar 1-6 bar ASTM I conductivity: µs/cm µs/cm Resistance at 25 C: 18.2 MW cm 18.2 MW cm TOC Value: 5-10 ppb 5-10 ppb Bacterial content in CFU/ml: < 1 < 1 Particle content: < 1 < 1 Endotoxines in EU/ml: ASTM II conductivity: µs/cm µs/cm Resistance at 25 C: MΩ cm MΩ cm Supply voltage: Automatic voltage regulation to 24 V Power consumption: 0.06 kw 0.06 kw Connector size: R 3/4" R 3/4" Dimensions, W x D x H in mm: 305 x 400 x x 400 x 545 Weight: 22 kg 22 kg Cat. no., 3 l/h version: Cat. no., 6 l/h version: * supplemented with an ultrafilter ** supplemented with UV-oxidation, 185/254 nm Consumables and accessories Wall-mount Cat. no Pretreatment module with RO-membrane, 3 l/h Cat. no Pretreatment module with RO-membrane, 6 l/h Cat. no Ultrapure water cartridge Cat. no Sterile filter Cat. no Vent filter for tank Cat. no TKA Smart2Pure UV** TKA Smart2Pure UF/UV 3 l/h or 6 l/h 3 l/h or 6 l/h 1.0 l/min 0.6 l/min 1 6 bar 1 6 bar µs/cm µs/cm 18.2 MW cm 18.2 MW cm 1 5 ppb 1 5 ppb < 1 < 1 < 1 < µs/cm µs/cm MW cm MΩ cm Automatic voltage regulation to 24 V 0.06 kw 0.06 kw R 3/4" R 3/4" 305 x 400 x x 400 x kg 22 kg Rights reserved for changes and printing errors. Printed in EU. Status GT Your TKA Representative TKA Wasseraufbereitungssysteme GmbH Stockland 3 D Niederelbert Telephone: +49 (0) / Telefax: +49 (0) / info@tka.de

67 Everyday ultrapure water. Even for demanding lab applications. Compact high-tech! TKA Smart2Pure 12. Two in one. ASTM Types I/II water. Instantly and fresh. WATER PURIFICATION SYSTEMS

68 TKA Smart2Pure 12. The password for converting normal tap water to ultrapure water. ASTM Type I Ultrapure water. For example, for: Molecular biology, microbiology, PCR, inorganic and organic trace analysis HPLC, ICP, TOC-analysis ASTM Type II Ultrapure water. For example, for Rinsing laboratory glassware Preparing and diluting buffers, reagents, tissue culture media and dyes Sample preparation for analytical methods such as flame AAS More ideas = more benefits! How TKA Smart2Pure 12 differs from other compact class systems: Powerful performance! Permeate flow 12 l/h Flow rate 1.0 l/min Lasting economy! Water purification progresses through separate modules, each with quick connects for simple individual replacement Module 1 Pretreatment module with integrated reverse osmosis membrane Module 2 Cartridge containing high-quality ultrapure resins with very long service intervals Easiest to operate! Just press a button to get ultrapure water no adjustments necessary, the microprocessor control is a selfcontrolling component Ultrapure water quality is ensured by the limiting value input RS 232 Interface The display can be tilted to your optimal reading position Positioning anywhere in the laboratory Laboratory bench-top operated or wall-mounted Usable worldwide! Whether 220/230 V, 110/120 V or other voltages and even with fluctuating voltages the automatic voltage regulator continually supplies the system with a constant 24 volts

69 TKA Smart2Pure 12. Ultrapure water at the press of a button! Position it, connect it, use it! Economical! Two long-life cartridges. Each requires replacement only when really necessary! One, two, three rapid cartridge change due to quickconnects. Space saving! Simply stand it on the lab bench, or mount it directly to the wall. High performance filter! Option of an integrated ultrafilter, generously dimensioned for pyrogene-free and nuclease-free water. The automatic self-cleaning gives years of service. UV Oxidation 185/254 nm! Optionally with additional UV oxidation for reducing the content of microorganisms and their metabolites.

70 Flow chart TKA Smart2Pure 12 UV/UF with external tank. The result: Ultrapure water of the quality required: ASTM Type I Production of ultrapure water for low volume requirements. The ultrapure water is delivered via a sterile filter. Conductivity in µs/cm: Resistance in MW cm at 25 C: 18.2 Bacterial content in CFU/ml: < 1 Particle content: < 1 Flow rate per minute: 1.0 litre Absolutely sterile! Ultrapure water is dispensed through a standardly built-in sterile filter. ASTM Type II 12 litres per hour. Ready-to-take at any time from the 30 or 60 litre tank. Conductivity in µs/cm: Resistance in MW cm at 25 C: Percentage of germs, bacteria, particles retained by the membrane: 99 % Flow rate per minute: 1.0 litre Convenient Swivel-mounted display always easy to read!

71 TKA Standard storage tanks. For 30 or 60 liters. Store high purity water safely and conveniently! An advanced and proven tank construction with many beneficial features: Large opening For easy and effective cleaning by hand. Closed with a screw cap. Sterile venting filter/tank overflow Contamination by microorganisms in the environment is prevented by two safety filters Option: A CO 2 absorber that prevents TOC value increases from drawn-in CO 2 Polyethylene tank Ultrapure water resistant, opaque to light and food-safe High purity water dispensing tap With 24 cm free space for practical water drawing Recirculation pump The recirculation pump protects the high purity water from bacterial growth during standstills and maintains the low conductivity value Wall mount For simple and space-saving wall mounting Standard storage tanks Volume: 30 liters 60 liters Material: PE acc. ME 8159 and NG2431 E, ultrapure water resistant, opaque to light, food-safe Outlet tap: Overflow: Diameter: 240 mm free headroom d8 hose connector 388 mm Height: 606 mm 920 mm Weight in kg 6 7 (Standard): cat. no Complementary accessories: Sterile owerflow CO 2 absorber + Sterile venting filter, 0.2 µm sterile venting filter, 0.2 µm UV Immersion lamp for Tank Wall mount for 30 l storage tank Wall mount for 60 l storage tank Conical bottom outlet Allows complete draining and efficient cleaning and disinfection

72 Technical Specifications TKA Smart2Pure 12 Standard TKA Smart2Pure 12 UF* Permeate performance at 15 C: 12 l/h 12 l/h Flow rate: 1.0 l/min 0.6 l/min Operating pressure in bar, min/max: 1 6 bar 1 6 bar ASTM I conductivity: µs/cm µs/cm Resistance at 25 C: 18.2 MW cm 18.2 MW cm TOC Value: 5 10 ppb 5 10 ppb Bacterial content in CFU/ml: < 1 < 1 Particle content: < 1 < 1 Endotoxines in EU/ml: ASTM II conductivity: µs/cm µs/cm Resistance at 25 C: MΩ cm MΩ cm Supply voltage: Automatic voltage regulation to 24 V Power consumption: 0.06 kw 0.06 kw Connector size: R 3/4 R 3/4 Dimensions, W x D x H in mm: 305 x 300 x x 300 x 545 Weight: 22 kg 22 kg cat. no., 12 l/h version: * supplemented with an ultrafilter ** supplemented with UV-oxidation, 185/254 nm TKA Smart2Pure 12 UV** TKA Smart2Pure 12 UF/UV 12 l/h 12 l/h 1.0 l/min 0.6 l/min 1 6 bar 1 6 bar µs/cm µs/cm 18.2 MW cm 18.2 MW cm 1 5 ppb 1 5 ppb < 1 < 1 < 1 < µs/cm µs/cm MW cm MΩ cm Automatic voltage regulation to 24 V 0.06 kw 0.06 kw R 3/4 R 3/4 305 x 300 x x 300 x kg 22 kg Consumables and accessories Pre-treatment (hardness stabilizing/activated carbon/prefilter 5 µm) cat. no Wall mounting bracket for Smart2Pure 12 cat. no Pretreatment module with RO-membrane, 12 l/h cat. no Ultrapure water cartridge cat. no Sterile filter cat. no Rights reserved for changes and printing errors. Printed in EU. Status x000 Your TKA Representative TKA Wasseraufbereitungssysteme GmbH Stockland 3 D Niederelbert Telephone: +49 (0) / Telefax: +49 (0) / info@tka.de

73 Ultra pure water, 18,2 MΩ cm. TKA GenPure Pro More Flexibility for Life Sciences, Analyses and Laboratory applications. WATER PURIFICATION SYSTEMS

74 TKA GenPure Pro. Ultra pure water 18,2 MΩ cm, TOC 1 5 ppb. Assured quality. Even for the most demanding and sensitive applications. With UV photo-oxidation and optional TOC monitoring! In the Biosciences For molecular biology and microbiology work In the preparation of cell and tissue cultures In PCR, DNA sequencing Electrophoresis In Analytical Chemistry Organic and inorganic trace analysis HPLC GC-MS, ICP-MS, GF-AAS TOC Measurements, IC In Routine Laboratory Work Analytical procedures IC, AAS, ICP-OES Norms All TKA Genpure systems exceed the norms ASTM type I, ISO 3696 Grade 1, ASTM D 1193, CLSI. TKA GenPure Pro are ultrapure water systems suitable for the most demanding laboratory applications. Withdrawl of the ultra pure water by the new flexible dispenser. TKA GenPure Pro UV with UV photo-oxidation. TKA GenPure Pro UV/TOC with UV photo-oxidation 185/254 nm and display of TOC values. Ultra pure water. There where you need it. With a small footprint GenPure Pro can be stood on the lab bench or, if preferable, it can be hung on a wall (without the need for extra mounting material). Independent from the place of installation the new flexible dispenser offers a maximum freedom while dispensing ultra pure water.

75 Intelligent technology that simplifies your work. Day for day! Flexible and comfortable! Dispensing for a variety of tasks New flexible dispenser within a radius of 60 cm of the instrument. For filling larger vessels or easy glassware washing. Easily readable The ergonomically designed control/dispenser unit can be tilted for optimal key pressing and dazzle-free readability. Illuminated four-line alpha-numeric display. Ready to use Feed water pressure reducer, UV photo-oxidation, polisher, sterile filter - all included as standard. Exact dosing Ultra pure water simply at the push of a button. Automatic volume control from litre with excellent accuracy of < 0,5%. Precise dispensing of even small volumes due to intelligent pump control. Simple to use Menu-driven operation with clear text displays of all functions and performance parameters. Choice of English, German or French. GLP Documentation Real-time clock and code protected operating system prevents unauthorized changes and settings. RS 232 Interface with adjustable sending intervals. Powerful ultra pure water set! The ultra pure water set is very generously dimensioned. So it combines a long service life with cost saving. The display gives notice of when it should be replaced. Rapid cartridge replacement The Aquastop quick connections of the ultra pure water set allows replacement within seconds during operation.

76 With the greatest accuracy! With highest security! Measurement of conductivity By two high precision measuring cells. With fully automatic check and calibration prior to each measurement via built-in reference resistance. Temperature measurement With temperature compensation that can be switched off according to USP <645>. Online TOC measurement at the dispensing point Real time TOC monitoring for continual measurement of organic substances in the water according to USP <643>. Intensity monitoring A photo-sensor continually checks the intensity of the UV lamp. A decrease in the measured UV radiation that would lead to an incorrect TOC measurement result is directly indicated in %. Visible ultra pure water quality. The measurements of conductivity and real-time TOC value are made directly prior to the dispensing valve. The display shows the most important measured values during ultra pure water dispensing. For example: Integrated feedwater monitoring Good quality feedwater is a basic requirement for the degree of purity of the water produced, as well as for the long service lives of all purification stages. An additional measuring cell permanently monitors the conductivity of the feedwater. Any exceeding of the limiting value is immediately displayed. The feedwater must always be included in a proper validation of an ultra pure water system. UV Photo-oxidation 185/254! A high performance UV unit with intensity photo sensor serves for a reliable reduction in the content of micro-organisms and their metabolites. The top-grade UV lamp is made of synthetic quartz glass for the greatest light yield of exactly the right wavelengths. It is held in a robust and corrosionresistant ANSI 316 L stainless steel housing. Optional PoD Ultrafilter! The new point-of-dispensing ultrafilter ensures endotoxine-free water for all biological applications. For safe and quick production of RNase- and DNase-free water whenever needed. Replacement items and accessories Replacement UV-lamp Ultra pure water cartridge Sterile filter, 0.2 µm PoD ultrafilter Disinfection cartridge Disinfection kit, pack of Log printer with RS 232 interface Qualification documents according to GLP

77 Feedwater requirements for TKA GenPure Pro We recommend distilled water or tap water that has been pretreated by flow through a DI type TKA ion exchanger or a TKA Pacific water purification system with connected 30, 60 or 100 litre storage tank. Please request our TKA Pacific UP/ UPW and TKA ion exchange brochures. Feedwater specifications: Colloid index (SDI): max. 1 Conductivity: < 5 µs/cm TOC value: max. 50 ppb Free chlorine: max ppm Turbidity: < 1.0 NTU Carbon dioxide: max. 30 ppm Silicate: max. 2 ppm Pressure: bar Ambient temp.: +2 C +35 C Flow chart TKA GenPure Pro UV-TOC UV Photo-oxidation 185/254 nm UV photo-oxidation reactor with specific geometry for even distribution of UV light of 185 nm and 254 nm wavelengths. 254 nm UV light kills bacteria by destroying the DNA in their cells. The bacterial content within the system is minimized. The combination of the two wavelengths produces hydroxyl free radicals that are extremely reactive. They oxidize and cleave small organic substances that are otherwise difficult to remove. The cleavage products are removed in the next purification stage. Adsorption + Ion exchange Polisher cartridge with a combination of high purity adsorptive material and an ion exchanger. The combination of adsorption and ion exchange removes the smallest organic molecules, such as the UV photo-oxidation cleavage products, as well as remaining inorganic ions. Membrane filter/pod ultrafilter Validated sterile filter of 0.2 µm pore size in a large area capsule with vent valve and protective bell. Sterile filter for the reliable removal of bacteria and particles directly prior to ultra pure water dispensing. Suitable for sterilization. The point-of-dispensing ultrafilter predominately serves the production of pyrogen- and nuclease-free water. The result: Ultra pure water of ASTM Type 1 quality. TKA ultra pure water system GenPure Pro UV GenPure Pro UV-TOC Flow rate in l/min: up to 2.0 up to 2.0 Conductivity in µs/cm: Resistivity, in MW cm at 25 C: TOC value in ppb: Bacteria in CFU/ml: < 1 < 1 Particulates, > 0,22 µm/ml: < 1 < 1 Endotoxines in EU/ml: RNase* in ng/ml: DNase* in pg/µl: Voltage: 230 V/ 50 Hz 230 V/ 50 Hz Power consumption: 0.1 kw 0.1 kw Interface: RS 232 RS 232 Dimensions W x D x H in mm: 472 x 330 x x 330 x 615 Weight in kg: Cat. no.: * with optional PoD ultrafilter

78 Qualification and Maintenance Technical support in the qualification of your TKA GenPure system The assembly, installation, start-up, operation and maintenance of the system are explained in detail in the Operating Instructions. Proper operation ensures a constant production of ultra pure water within the given specifications for a long time. The Operating Instructions are also an extremely important basis for the qualification of the system. The TKA Qualification Manual This is designed to assist you in qualifying your system. A series of steps lead through Design Qualification (DQ), Installation Qualification (IQ) and Operating Qualification (OQ) to the final evaluation of the qualification documents. Maintenance We recommend that you sign a maintenance contract with your TKA authorized customer service company for the carrying out of all calibration and maintenance work. You then have the assurance that the GLP-conformity and high operational safety and reliability of your system will be maintained. Änderungen und Druckfehler vorbehalten. GP-FleX01en Status x000 Go Your TKA authorized specialist distributer: TKA Wasseraufbereitungssysteme GmbH Stockland Niederelbert / Germany Telefon: 02602/ Telefax: 02602/ info@tka.de