In Situ Mass Spectrometry: Underwater Measurements & Miniaturization
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1 In Situ Mass Spectrometry: Underwater Measurements & Miniaturization R. Timothy Short, Strawn K. Toler, Friso H.W. van Amerom, Ashish Chaudhary, Peter G. Wenner, Ryan J. Bell, L. Diego Miranda and Robert H. Byrne SRI St. Petersburg & University of South Florida College of Marine Science Polar Technology Conference
2 In Situ Mass Spectrometer Activities at USF & SRI St. Petersburg Underwater Membrane Inlet MS Variety of Deployments Cylindrical Ion Trap MS Extreme Miniaturization
3 MS: Versatile Chemical Sensor Trace Elements Isotope Ratios Pollutants/VOCs Dissolved Gases Proteins/Amino Acids Bacterial Signatures No configuration valid for all analytes
4 Membrane Inlet Mass Spectrometry (MIMS) Ion source Electron impact Mass-filter Quadrupole Detector Electron multplier Membrane e Ion and fragments Mass-scan
5 Principle Features of UMS Type Linear quadrupole mass filter Mass range 200 amu Inlet System Membrane introduction system Power consumption Watts Voltage of operation 24 VDC or 110 VAC Deployment time Configuration dependent Dimensions 19 cm (7.5 ) L 105 cm (41 ) Weight 33 kg (72.7 Lb) Depth >1000 m DSL tether range ~1600 m (1 mile)
6 New 200 amu In Situ Mass Spectrometer Microcontroller Embedded PC MS electronics box 200 amu linear quadrupole in vacuum housing w/ heating jacket Turbo pump MIMS probe Roughing pump
7 Simultaneous Detection of Multiple Analytes Dissolved Gases e.g. Nitrogen, Oxygen, Argon, Carbon Dioxide, Methane, Hydrogen Sulfide Volatile Organic Compounds e.g. Toluene, Benzene, Dimethyl Sulfide, Chloroform Larger MW Compounds with Modification e.g. PCBs, Pesticides, Drugs, Toxins
8 Calibration - Instrument Parameters Physical parameters that affect instrument response: Detector settings Filament settings Residual gas Membrane geometry Membrane temperature Sample velocity Constant during deployment Hydrostatic pressure Variable during deployment
9 Calibration - Method Two solutions with known gas concentrations are mixed at various ratios to allow for intermediate concentrations and in situ automated calibration Shipboard apparatus allows in-field sample preparation and calibration
10 Calibration - Screenshot Nitrogen (m/z 28) Oxygen (m/z 32) Methane (m/z 15) Calibration Normalized at 200atm 1atm Response (78% N2, vs Pressure 21% O2, 1% (atm) CH4) 2/18/05 2/18/05 Instrument Normalized Response Response (A) Instrument Response (A) 1.00E E E E E E E E E E E E-08 Oxygen y = 1.742E-10x E-08 R 2 = 9.999E-01 Oxygen y = 7.056E-11x E-08 R 2 = 9.994E-01 Nitrogen y = 1.821E-10x E-09 R 2 = 9.999E E E-08 Methane Nitrogen E-08 Nitrogen 3.00E-08 Oxygen y = 7.176E-11x E E-08 R 2 = 9.939E E E E-08 y = 1.593E-10x E-10 Methane y R= E-11x = 9.995E E E+00 Methane R 2 = 9.962E E Concentration (umole/kg) Hydrostatic Concentration Pressure (umole/kg) (atm)
11 1.00E E E E E E Scan # 2. 2 e e e e e e e e e e e : 0 0 F r id a y S c a n N u m b e r ( 7 s e c / s c a n ) 1 7 :0 0 S a tu rd a y Deployment Methods W a s t e W a t e r I n f lu e n t m / z 9 1 Intensity (Amps) AUV USV Moored Intensity ROV Depth Profiling Diver
12 7.00E E E E-09 IntensityO2 3.00E-09 CO 2.00E E E+00 Lake Maggiore Chemical Surveys Mass spectrometer deployed aboard an unmanned surface vehicle Mapping variation of dissolved gas ion intensities Develop surface contour maps based on gas ion intensity data Carbon dioxide & oxygen data displayed Scan number
13 Lake Maggiore, St Petersburg, FL O 2 and CO 2 are inversely correlated in areas of active photosynthesis and respiration m/z 32 - Oxygen m/z 44 - Carbon Dioxide
14 Depth Profiles of Dissolved Gases Vertical profiles of dissolved gases with underwater mass spectrometer in Gulf of Mexico Mount instrument on custom frame along with CTD, DO and ph Sensors Communicate with instrument through standard UNOLS CTD tether using Seabird Modem Determine dissolved gas concentrations from mass spec data with the aid of a portable calibration unit
15 Pre-Deployment Calibration
16
17 Depth Profile Data (MC118)
18 Future Plans for Underwater MS Deployment Opportunities Methane Hydrates: Gulf of Mexico Subglacial Antarctic Lake Environments AUV in Extreme Environments Alternative Sampling Interfaces Wider range of analytes Unattended Operation/ Full Ocean Depths Ocean Observatories Extreme MS Miniaturization MEMS Microfabrication
19 Miniaturization of Ion Trap Mass Spectrometers Hyperbolic Electrodes Quadrupole Potential Mass Instability Scan
20 Cylindrical Ion Trap MS Arrays Hyperbolic Surfaces Cylindrical Geometry Miniaturize Microtrap Lower Sensitivity Array Increased Sensitivity Microfab Tolerances Cylindrical Surfaces
21 Advantages of Miniaturization of Mass Analyzer Lower voltages/lower power Reduced vacuum requirements Overall system reduction Reduced cost MS sensor networks
22 Microfabricated CIT Arrays (MEMS) Silicon Wafer Process flow Cylindrical ion trap
23 SEM of Half-Array of Micro-Cylindrical Ion Traps in Silicon Fabricated CIT Half-Ring Electrodes with Endplates
24 Packaging Method for Micro-CITs 6 x 6 Micro-CIT Array Gold plated PCB for mounting and electrical connections
25 Spectrum of TCE recorded with micro Cylindrical Ion Trap (r o = 360 microns) Trichloroethylene Volts m/z Ejection time
26 Miniaturization and Integration of Peripherals GC column Pump Inlet system Ionization source Detector Volume of vacuum chamber, overall size and power consumption drastically reduced
27 Future Plans for Micro MS Optimize performance of micro-cit array Optimize and integrate ion source arrays Fabrication and integration of other components: Detectors Vacuum and sampling systems Pre-separation stage (e.g., GC) Handheld MS sensor Merge with Underwater MS program
28 Acknowledgements Faculty, staff and students at the USF Center for Ocean Technology and College of Marine Science Staff at USF MEMS Facility Funding from U.S. Office of Naval Research (ONR) Grant No. N and U.S. Army Space and Missile Defense Command (SMDC) Grant No. DASG60-00-C-0089.
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