NEW. NIST Traceable UV/Vis/NIR Reference Sets USP 857

Transcription

1 NEW USP 857 REFERENCES (PAGES 14, 18, 23, 38) Winter 2015/16 NIST Traceable UV/Vis/NIR Reference Sets Accredited to ISO/IEC and ISO Guide 34 including Instrument Qualification to new USP Chapter <857> A USB Drive supplied with each set contains the calibration certificate and all necessary user information. Hard copy available on request.

2 Why do you need to use reference materials? Ultraviolet, visible and NIR spectrophotometers are fundamentally accurate, however it is vital to check instrument performance on a regular basis to ensure operation is within acceptable parameters. Certified references provided by Starna are based on materials, which have been used or produced by a National Metrology Institute (NMI), such as the National Institute of Standards and Technology (NIST) or fundamental metrological principles. When used in routine testing of instrument performance, a quality control database of the operating characteristics for a specific instrument can be built. This database can then be used for the following purposes: What makes Starna reference materials unique? * Demonstration of satisfactory instrument performance to any inspection or regulatory agency. * Detection of instrument malfunction. * Provide data to a service technician allowing detection of instrument faults and assistance in repair. * Construction of Process Control Charts Each spectrophotometer has specific operating characteristics based on the geometry and basic design of the optical system. These reference materials become your guide to the efficient operation of your instrument. References are supplied in either heat fusion sealed quartz cells or, in the case of the glass or Metal on Quartz filters, in an aluminum holder with the same external dimensions as a standard spectrophotometer cell. All sealed cells, glass filters or Metal on Quartz reference materials produced by Starna are Ready to Use. Starna's sealed references are environmentally friendly as they eliminate the need to open an ampoule, use it once and throw it away. Wet chemistry methods are no longer required to make up the references from raw materials. With care, the sets will last for many years. All certified sets can be recertified. Properties of Starna heat fusion sealed quartz cells, glass or Metal on Quartz references: 1) Sealed cells ensure cleanliness, repeatability and reliability 2) They can be used multiple times over many years 3) The range covers the Far UV, UV, Visible and NIR 4) All calibrated & NIST traceable references can be recertified. Construction of reference materials Starna has almost fifty years of experience pioneering the technology of sealing reference materials into quartz spectrophotometer cells. The technology allows the use of materials in the UV range where glass filters are not usable. The sealed reference cells have the same optical characteristics as cells used in normal analysis thus removing many variables, which might be introduced using any other form of optical configuration or reference preparation. The references have a lifetime guarantee subject to specified conditions and are supplied in suitable storage containers. The glass filters are made to the highest industry standards with raw material of known characteristics. UKAS ISO/IEC Accredited / NIST Traceable NIST (National Institute for Standards and Technology in Washington DC, USA) traceability applies to all our reference materials, unless otherwise indicated. Traceability is established during the calibration of each of our five reference spectrophotometers using NIST primary standards, both before and after calibrating Starna reference materials. Documentation supplied with Starna references details the analysis procedure, NIST traceability, the analyzed values of the reference and the confidence limits. Certification supplied with Starna reference materials is suitable for use with all inspecting agencies when used in the context of standard operating procedures for quality control, established in your laboratory. Our calibration laboratory is inspected and accredited by UKAS (United Kingdom Accreditation Service) under ISO/IEC for the Starna Calibration Laboratory and under ISO Guide 34 as a reference material producer. This level of accreditation assures the user that the certification of our reference materials is of the highest standard and may be used to validate instrument performance in all ISO/IEC regulated environments. Should I use accredited NIST Traceable References? Yes, to comply with USP <857>! 2 USP <857> states that: CRMs should be obtained from a recognized accredited source and include independently verified traceable value assignments with associated calculated uncertainty. Virtually all the Certified Reference Materials in this catalog are traceable to NIST, as shown opposite. The raw materials used to prepare the references are either purchased from NIST (where available) or directly tested against a NIST supplied reference. The instruments used to generate the certified values and uncertainties are themselves qualified against NIST primary standards or SRMs. The most important element of NIST traceability is the Starna certificate of analysis and traceability. This certificate details the method of analysis and the certified results. You can use these certified results as the basis of your quality program. If your laboratory is routinely monitored by a certifying agency, then the Starna level of accreditation and NIST traceability will meet all your regulatory requirements.

3 Table of Contents: Ultraviolet References Material: Used for: Traceable to: Configuration: Page(s): Potassium Dichromate Absorbance, Linearity, UV SRM 935a Filled Quartz Cell 4, 5, 6 & 7 Nicotinic Acid Absorbance, Far UV SRM 935a Filled Quartz Cell 8 Starna TS8 Deep UV, Wavelength, Absorbance Filled Quartz Cell 9 Metal on Quartz Filters Absorbance, Linearity, UV/VIS SRM 2031 Metal Coated Quartz Window 10 Starna Green Absorbance, Wavelength, Linearity UV/VIS SRM 935a/2034 Filled Quartz Cell 11 Holmium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 12 Didymium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 12 Samarium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 13 Rare Earth Oxide Wavelength Far UV SRM 2034 Filled Quartz Cell 13 Sodium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell Potassium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell Potassium Iodide Stray light UV SRM 2032 Filled Quartz Cell Sodium Iodide Stray light UV SRM 2032 Filled Quartz Cell Lithium Carbonate Stray light UV SRM 2032 Filled Quartz Cell Sodium Nitrite Stray light VIS SRM 2032 Filled Quartz Cell Acetone Stray light VIS SRM 2032 Filled Quartz Cell Toluene in Hexane Resolution SRM 935a Filled Quartz Cell 18 Tolene in Methanol Bandwidth derivative spectroscopy SRM 935a Filled Quartz Cell 19 Benzene Vapor Resolution Filled Quartz Cell 20 Visible References Material Used for Traceable to: Configuration: Page: Holmium Glass Filter Wavelength VIS SRM 2034 Glass Filter 21 Didymium Glass Filter Wavelength VIS SRM 2034 Glass Filter 21 Neutral Density Glass Filters Absorbance, Linearity, VIS SRM 930e Glass Filters 22, 23 Microplate Reader References Material Used for Traceable to: Configuration: Page: Glass Filters Wavelength, Absorbance, VIS SRM 2034, 930e Glass Filters 24 Adaptor To use standard references in Microplate Readers 25 DNA and RNA 260/280 Ratio References Material Used for Traceable to: Configuration: Page: Stable Aqueous 260/280 Absorbance UV SRM 935a Filled Quartz Cell 26 Stable Aqueous 260/280 Absorbance UV SRM 935a Vials, Screw Top 27 Near Infrared Material Used for Traceable to: Configuration: Page: Neutral Density Filters Absorbance, Linearity, NIR SRM 2031 Glass Filters 28 Metal on Quartz Filters Absorbance, Linearity, NIR SRM 2031 Metal Coated Quartz Window 29 NIR Wavelength material Wavelength, NIR SRM 2065 Filled Quartz Cell 30 Chloroform Stray Light, NIR SRM 2065 Filled Quartz Cell 31 Polystyrene Reference Wavelength, Mid IR/Near IR-FTIR SRM 1921b, 2065 Mounted Film 32, 33 Fluorescence References Material Used for Traceable to: Configuration: Page: Ultra High Purity Water Fluorescence Instrument Sensitivity Filled Quartz Cell 34 Quinine Sulfate Fluorescence Wavelength, SRM 936a Filled Quartz Cell 35 Raman References Material Used for Configuration: Page: Cyclohexane Raman Wavelength Filled Quartz Cell 36 Calibration Services Page: History, expected parameters, certification format, capabilities, scope 37 Qualification Sets for easier Compliance (USP, EP, ASTM, etc.) Page: US Pharmacopeia 38-9, 41 European & other Pharmacopoeia References for International Compliance References described in this catalog comply with the requirements for instrument qualification of UV and visible spectrophotometers recommended by national and international regulatory authorities worldwide including: United States Pharmacopeia European Pharmacopoeia American Society for Testing and Materials British Pharmacopoeia USP EP (Ph. Eur) ASTM BP Deutsches Arzneibuch (German Pharmacopoeia) Therapeutic Goods Administration (Australia) Japanese Pharmacopoeia Pharmacopoeia of the Peoples Republic of China DAB TGA JP PPRC 3

4 Potassium Dichromate - UV - Absorbance/Linearity up to 1.5 A Description & NIST Traceability: Certified Wavelengths: Potassium dichromate from NIST (SRM 935a), permanently sealed by heat fusion in quartz cells, NIST Traceable complete with UKAS ISO/IEC accredited certificate of calibration. Testing absorbance and linearity scale in the UV region of the spectrum up to 1.5 absorbance. 235, 257, 313 and 350 nm for 20 mg/l to 100 mg/l. 430 nm for 600 mg/l. UV quartz cells that have been permanently sealed by heat fusion. Potassium dichromate solvated in dilute perchloric acid is the recognized method for testing the absorbance scale and linearity of spectrophotometers in the UV. Solid potassium dichromate used by Starna is purchased directly from NIST (SRM 935a). Solutions are made up in accordance to NIST certification and then permanently sealed by heat fusion in Far UV quartz spectrophotometer cells. The filled cells are tested and certified against the expected values from NIST. A certificate is provided with each set of cells, which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for quality control, established in your laboratory. Each set is supplied with the sealed blank cell used to obtain the certified measurements. The following chart is a listing of approximate absorbance values for each concentration based on the wavelength at which the potassium dichromate cells are to be read: Concentration 235 nm 257 nm 313 nm 350 nm 430 nm 20 mg/l n/a 40 mg/l n/a 60 mg/l n/a 80 mg/l n/a 100 mg/l n/a European Pharmacopoeia compliance requires the following additional reference: 600 mg/l n/a n/a n/a n/a Absorbance (A) Potassium Dichromate Solutions Wavelengths (nm) 100 mg/l 80 mg/l 60 mg/l 40 mg/l 20 mg/l Spectral scan: The graph to the left is a scan of five different concentrations of potassium dichromate. It clearly shows the peaks and troughs in absorbance and the differences due to potassium dichromate concentration. Peaks: 257 and 350 nm Troughs: 235 and 313 nm Absorbance (A) Potassium Dichromate Linearity 20 mg/l 40 mg/l 60 mg/l 80 mg/l 100 mg/l Dichromate Concentrations 257 nm 235 nm 350 nm 313 nm The certificate supplied with the NIST traceable sets lists the absorbance values for each peak and trough at the four wavelengths measured against the perchloric acid blank at the four wavelengths. When measured in your spectrophotometer you can validate your transmittance/absorbance scale for accuracy and linearity using the set with five different concentrations and the blank. Linearity: Plotting concentration against absorption using the set of five cells against the blank, you can test your spectrophotometer for linearity through the UV range. The use of the NIST traceable set with five different concentrations and a blank is recommended for best results. 4

5 RM set: This NIST traceable set of one blank and five increasing concentrations of potassium dichromate is our most useful and widely purchased set consisting of 6 cells with the following materials: Cell# Containing Concentration 1 Perchloric acid blank 0.001M 2 Potassium Dichromate 20 mg/l 3 Potassium Dichromate 40 mg/l 4 Potassium Dichromate 60 mg/l 5 Potassium Dichromate 80 mg/l 6 Potassium Dichromate 100 mg/l Scan the absorbance of the potassium dichromate cells over the certified wavelength range, against the blank supplied. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration: if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the four wavelengths. If you determine that your instrument is not giving you the correct values, consult your service technician for advice on how to determine and correct any problem, which this reference may have detected. for expanded uncertainty budget and expected parameters see p 37 Linearity Dichromate set: Potassium Dichromate, 5 concentrations & Blank Cell 20, 40, 60, 80 & 100 mg/l & Blank Cell RM $ Linearity Dichromate set, with additional concentration for European Pharmacopoeia Compliance: Potassium Dichromate, 6 concentrations & Blank Cell 20, 40, 60, 80,100 & 600 mg/l & Blank Cell RM $ Single Concentrations: Potassium Dichromate, 20 mg/l & Blank Cell RM-02 $ Potassium Dichromate, 40 mg/l & Blank Cell RM-04 $ Potassium Dichromate, 60 mg/l & Blank Cell RM-06 $ Potassium Dichromate, 80 mg/l & Blank Cell RM-08 $ Potassium Dichromate, 100 mg/l & Blank Cell RM-10 $ Additional concentration required for European Pharmacopoeia Compliance: Potassium Dichromate, 600 mg/l & Blank Cell RM-60 $ Any combination of references, including those from page 7, can be ordered by combining the individual part numbers eg RM is 3 references 80, 160 and 240 mg/l and Blank : Potassium Dichromate, 1 concentration & Blank $ Potassium Dichromate, 2 concentrations & Blank $ Potassium Dichromate, 3 concentrations & Blank $ Potassium Dichromate, 4 concentrations & Blank $ Potassium Dichromate, 5 concentrations & Blank $ Potassium Dichromate, 6 concentrations & Blank $ Potassium Dichromate, 7 concentrations & Blank $

6 Potassium Dichromate - UV - Absorbance/Linearity up to 3.3 A Description & NIST Traceability: Certified Wavelengths: Potassium dichromate from NIST (SRM 935a), permanently sealed by heat fusion in quartz cells, NIST Traceable complete with UKAS ISO/IEC accredited certificate of calibration. Testing absorbance and linearity scale in the UV region of the spectrum up to 3.3 absorbance. 235, 257, 313 and 350 nm for 20 mg/l to 240 mg/l. UV quartz cells that have been permanently sealed by heat fusion. Absorbance (A) Potassium dichromate solvated in dilute perchloric acid is the recognized method for testing the absorbance scale and linearity of spectrophotometers in the UV. Solid potassium dichromate used by Starna is purchased directly from NIST (SRM 935a). Solutions are made up in accordance to NIST certification and then permanently sealed by heat fusion in Far UV quartz spectrophotometer cells. The filled cells are tested and certified against the expected values from NIST. A certificate is provided with each set of cells, which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for quality control, established in your laboratory. Each set is supplied with the sealed blank cell used to obtain the certified measurements Potassium Dichromate Solutions Wavelengths (nm) The following chart is a listing of approximate absorbance values for concentrations 120 mg/l or higher, at the wavelengths at which the potassium dichromate cells are calibrated: 240 mg/l 220 mg/l 200 mg/l 180 mg/l 160 mg/l 140mg/l 120 mg/l 100 mg/l Concentration 235 nm 257 nm 313 nm 350 nm 120 mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 60 mg/l 40 mg/l 20 mg/l Spectral scan: The graph to the left is a scan of 12 different concentrations of potassium dichromate. It clearly shows the peaks and troughs in absorbance and the differences due to potassium dichromate concentration. Peaks: 257 and 350 nm Troughs: 235 and 313 nm The certificate supplied with these NIST traceable sets lists the absorbance values for each peak and trough at the four wavelengths measured against the perchloric acid blank at the four wavelengths. Absorbance (A) Potassium Dichromate Linearity 257 nm 235 nm 350 nm 313 nm When measured in your spectrophotometer you can validate the transmittance/absorbance scale for accuracy and linearity through the UV range for up to 12 different concentrations and absorbance values up to 3.5 A. The use of the NIST traceable set with six different concentrations and a blank is recommended for best results mg/l 40 mg/l 60 mg/l 80 mg/l 100 mg/ 120 mg/ 140 mg/ 160 mg/ 180 mg/ 200 mg/ 220mg/l 240 mg/ 6 Dichromate Concentrations

7 RM set: This NIST traceable set of one blank and six increasing concentrations of potassium dichromate is a convenient route to instrument qualification at higher absorbance values, and contains seven cells with the following materials: Cell# Containing Concentration 1 Perchloric acid blank 0.001N 2 Potassium Dichromate 120 mg/l 3 Potassium Dichromate 140 mg/l 4 Potassium Dichromate 160 mg/l 5 Potassium Dichromate 180 mg/l 6 Potassium Dichromate 220 mg/l 7 Potassium Dichromate 240 mg/l An alternative set, RM , covers the whole absorbance scale from 0.2 A to 3.5 A. Scan the absorbance of the potassium dichromate cells over the certified wavelength range, against the blank supplied. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration: if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. For expanded uncertainty budget and expected parameters see page 37 To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the four wavelengths. If you determine that your instrument is not giving you the correct values, consult your service technician for advice on how to determine and correct any problem, which this reference may have detected. Linearity Dichromate set from 1.5 to 3.5A Potassium Dichromate, 6 concentrations & Blank Cell 120, 140, 160, 180 & 220 & 240 mg/l & Blank RM $ Linearity Dichromate set from 0.19 to 3.5A Potassium Dichromate, 6 concentrations & Blank Cell 40, 80, 120, 160, 200 & 240 mg/l & Blank RM $ Single Concentrations: Potassium Dichromate, 120 mg/l & Blank Cell RM-12 $ Potassium Dichromate, 140 mg/l & Blank Cell RM-14 $ Potassium Dichromate, 160 mg/l & Blank Cell RM-16 $ Potassium Dichromate, 180 mg/l & Blank Cell RM-18 $ Potassium Dichromate, 200 mg/l & Blank Cell RM-20 $ Potassium Dichromate, 220 mg/l & Blank Cell RM-22 $ Potassium Dichromate, 240 mg/l & Blank Cell RM-24 $ Any combination of references, including those from page 5, can be ordered by combining the individual part numbers e.g. RM is three references 80, 160 and 240 mg/l and Blank : Potassium Dichromate, 1 concentration & Blank $ Potassium Dichromate, 2 concentrations & Blank $ Potassium Dichromate, 3 concentrations & Blank $ Potassium Dichromate, 4 concentrations & Blank $ Potassium Dichromate, 5 concentrations & Blank $ Potassium Dichromate, 6 concentrations & Blank $ Potassium Dichromate, 7 concentrations & Blank $

8 Nicotinic Acid - Far UV - Absorbance/Linearity Description & NIST Traceability: Certified Wavelengths: Four concentrations of Nicotinic acid in dilute hydrochloric acid together with a blank, permanently sealed by heat fusion in quartz cells. NIST Traceable complete with UKAS ISO/IEC accredited certificate of calibration Testing absorbance scale and linearity in the Far UV region of the spectrum. 213 nm, 261 nm Far UV quartz cells that have been permanently sealed by heat fusion Nicotinic acid solvated in dilute hydrochloric acid is a well documented method for validation of the absorbance scale and the linearity of a spectrophotometer in the Far UV region. Prepared in 0.1M hydrochloric acid, nicotinic acid gives a spectral scan containing characteristic peaks at approx. 210 nm and 260 nm. Within the concentration range 5-25 mg/l, if the absorbance scale of a spectrophotometer at 2 nm SBW is linear, the absorbances of a series of concentrations will be a linear function of concentration, at the specified SBW. The filled cells are tested and certified against the calculated values. A certificate is provided with each set of cells, which shows the certified absorbance values and confidence limit for each cell. The certificate is suitable for use with inspecting agencies when used in the context of a standard operating procedure for the quality control established in your laboratory. The following is a list of approximate absorbance values for each concentration at 1 nm SBW* at the calibration wavelengths Nicotinic Acid Solutions Concentration 213 nm 261 nm mg/l 6 mg/l mg/l mg/l mg/l * Alternative SBW values available on request. Absorbance (A) mg/l 12 mg/l 6 mg/l Scan the absorbance of the nicotinic acid cells over the certified wavelength range against the blank supplied. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. for expanded uncertainty budget and expected parameters see p 37 Wavelength (nm) To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the two wavelengths. If you determine that your instrument is not giving you the correct values, consult your service technician for advice on how to determine and correct any problem, which this reference may have detected. Single Concentrations: Nicotinic Acid, 6 mg/l & Blank Cell RM-1A $ Nicotinic Acid, 12 mg/l & Blank Cell RM-2A $ Nicotinic Acid, 18 mg/l & Blank Cell RM-3A $ Nicotinic Acid, 24 mg/l & Blank Cell RM-4A $ Linearity Nicotinic Acid set: Nicotinic Acid, 4 concentrations & Blank Cell RM-1A2A3A4A $

9 Starna TS8* Deep UV - Wavelength/Absorbance nm Description & NIST Traceability: Certified Wavelengths: An ultra-stable solution permanently sealed by heat fusion in quartz cells. NIST traceable complete with UKAS ISO/IEC accredited certificate of calibration Testing absorbance scale and wavelength accuracy in the Deep UV region. 191, 209 and 226 nm. Far UV quartz cells that have been permanently sealed by heat fusion. Qualifying a UV spectrophotometer in the Deep UV presents particular problems associated with the short wavelengths, reduced energy and sensitivity as well as the variability in transmission characteristics of UV grade quartz below 200 nm. These problems have been overcome with this unique stable reference by using innovative design and manufacturing protocols. The reference consists of a TS8* solution, which has distinct absorption/transmittance and peak characteristics in the deep UV, supplied together with a solvent blank permanently sealed by heat fusion into physically identical Far UV quartz cells. In addition to absorbance qualification this reference is also certified for wavelength qualification. This allows user flexibility as the reference is supplied with certified wavelength and absorbance values at two different spectral bandwidths, 1.0 and 5.0 nm. The Starna TS8 Deep UV reference set of proven stability is supplied with a Certificate of Calibration valid for two years by the Starna Calibration Laboratory, which is accredited by UKAS under ISO/IEC 17025, and ISO Guide 34. *TS8 is a proprietary matrix with the necessary spectral characteristics and stability to be used as a reference material. Deep UV (DUV) Reference Absorbance (A) Wavelength (nm) The peak wavelengths should first be identified by performing a wavelength scan at over the range from 190 to 260 nm at the required spectral bandwidth. These peaks should be verified as being within the expected wavelength tolerance. The spectrophotometer absorbance values at these peak wavelengths can then be measured and compared to the certified absorbance values taking the expanded uncertainty budget of for expanded uncertainty budget and expected parameters see p 37 the certified references into consideration. If the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. If it is determined that your instrument is not giving you the correct values, consult your service technician for advice on how to determine and correct any problem, which this reference may have detected. Starna TS8, Deep UV & Blank Cell RM-DUV/195 $

10 Metal on Quartz UV/Vis Absorbance/Linearity Description & NIST Traceability: Certified Wavelengths: Metal on Quartz Visible filter for absorbance and linearity in the UV and Visible area of the spectrum. NIST Traceable complete with UKAS ISO/IEC accredited certificate of calibration 250, 280, 340, 360, 400, 465, 500, 546.1, 590 and 635 nm at spectral bandwidth of 5 nm or less. Metal coated quartz filter with coated side protected by an optically contacted quartz window. The filters are mounted in protective aluminum holders that will fit a standard instrument cell holder. The Starna RM-1Q3Q9Q is a direct equivalent to the NIST SRM 2031, with a standard range of Metal on Quartz absorbance standards for the UV/Visible area of the spectrum. All Starna Metal on Quartz filters have an optically contacted quartz cover plate to protect the metalized surface and facilitate easy cleaning. This was also a feature of the original NIST SRM The filters allow absorbance and linearity at the ten wavelengths across the range and the relative values are shown on the table below. They can be certified at wavelengths other than those RM-1Q3Q9Q (SRM 2031 Equivalent) shown above, on request. The filters are manufactured in our ISO 9001 accredited production facility, under ISO Guide 34 as a reference material producer and calibration is carried out in our ISO/IEC accredited calibration laboratory. The UV/Visible Metal on Quartz filters have a relatively flat profile across all UV/Visible wavelengths. Normally three representative absorption filters would be chosen for any given application but the filters may be bought individually, in sets as shown below or any combination from the standard range. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the Abosrbance (A) Metal on Quartz Metal on Quartz Filters: Part No. %T Transmittance Absorbance RM-Q1 1% 2.00 A RM-Q3 3% 1.52 A RM-1Q 10% 1.00 A RM-3Q 30% 0.52 A RM-5Q 50% 0.30 A RM-9Q 90% 0.06 A absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. It is important to confirm that Metal on Quartz filters are suitable for use with your instrument as they have a highly reflective surface and some instruments are sensitive to back reflection within the sample compartment. 550 Wavelength (nm) Q1 Q3 1Q 3Q 5Q 9Q 10 for expanded uncertainty budget and expected parameters see p 37 Ordering Information (Let us know what combination you require): Single Metal on Quartz Reference, & Blank Holder RM-XX e.g. RM-3Q $ Two Metal on Quartz References, & Blank Holder Sets can be constructed by combining the part numbers $ Three Metal on Quartz References, & Blank Holder e.g. RM-1Q3Q9Q (Equivalent to NIST 2031a) $ Prices for other quantities available on request

11 Starna Green Broadband - Wavelength, Absorbance/Linearity Description & NIST Traceability: Certified Wavelengths: A mixed ultra stable dye solution permanently sealed by heat fusion in quartz cells. NIST traceable complete with UKAS ISO/IEC accredited certificate of calibration Testing wavelength accuracy, absorbance scale and linearity in UV/Vis region. 257, 416, and 630 nm with a SBW up to 12 nm The use of stable aqueous dye solutions is an established and well recognized method for the validation of the absorbance scale and linearity of a spectrophotometer. When prepared at the appropriate concentrations, these references have spectral characteristics that allow both the certification of the wavelength (257, 416, and 630 nm) and absorbance scale (0.25, 0.5, 0.75 or 1.0 A) in one discrete reference. Within the concentration range available, if the absorbance scale of the spectrophotometer is linear, the apparent absorbencies of a series of concentrations will be a linear function of concentration. Far UV quartz cells that have been permanently sealed by heat fusion. Also available in 1.5 ml screw-cap vials Starna Green is also available in 1.5 ml screw cap vials, for use in microvolume devices. The concentration 10X is matched to give absorbance readings of approximately 1A at a 1 mm path length. The Starna Green reference is supplied with a Certificate of Calibration valid for two years by the Starna Calibration Laboratory, which is accredited by UKAS under ISO/IEC 17025, and ISO Guide 34. Starna Green Absorbance (A) S S S S - 25 Scan the absorbance of the Starna Green cells over the certified wavelength range against the blank supplied. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget of the certified references into consideration, if the absorbance values fall within the expected parameters of your instrument then your instrument is working correctly. for expanded uncertainty budget and expected parameters see p 37 Wavelengths (nm) To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the three wavelengths. If you determine that your instrument is not giving you the correct values, consult your service technician for advice on how to determine and correct any problem, which this reference may have detected. Starna Green, Blank, 25X concentration RM-1SG $ Starna Green, Blank, 50X concentration RM-2SG $ Starna Green, Blank, 75X concentration RM-3SG $ Starna Green, Blank, 100X concentration RM-4SG $ Starna Green, Blank, 4 concentrations RM-1SG2SG3SG4SG $ Starna Green, 1 x 1.5 ml vial, screw cap RM-SG10-SC1 $ Starna Green, 2 x 1.5 ml vials, screw cap RM-SG10-SC2 $ Starna Green, 4 x 1.5 ml vials, screw cap RM-SG10-SC4 $

12 Liquid Wavelength References UV and Far UV Traceability: Description: All Starna liquid wavelength references are NIST traceable and supplied with UKAS ISO/IEC accredited certificate of calibration. Liquid references permanently sealed by heat fusion into far UV quartz cells. This ensures the optical configuration method used for quality control is identical to that for normal analysis. They also have narrower bandwidths than glass filters, providing more accurate location of the peaks. These solutions can be used to confirm that the wavelength scale of your instrument is within the manufacturer's tolerances for any given wavelength. Four references cover wavelengths from UV to Far UV. Spectral Band Width: Because the absorption bands are asymmetric, wavelength values will be dependent on spectral bandwidth. For this reason the certified wavelength values given below are approximate. The certificate supplied with each reference gives actual wavelength values measured at 0.1, 0.25, 0.5, 1, 2, and 3 nm. Values at other SBWs can be supplied on request. Holmium Oxide (Holmium oxide in perchloric acid) Certified Wavelengths: 241, 250, 278, 288, 334, 346, 361, 385, 417, 451, 468, 485, 537, 641 nm Holmium oxide (4% m/v) in 10% v/v perchloric acid, permanently sealed by heat fusion into a 10 mm far UV quartz cuvette. When prepared in perchloric acid, holmium oxide gives a spectral scan containing a series of 14 sharp and well-defined peaks covering the wavelength range from 240 to 650 nm. Absorbances (A) Holmium Oxide Solution It is the most widely used reference for validating the wavelength scale of UV/Vis spectrophotometers Wavelengths (nm) Didymium Oxide (Didymium oxide in perchloric acid) Certified Wavelengths: 298, 329, 354, 444, 469, 482, 512, 522, 575, 732, 740, 794, 799, 864 nm Didymium is a mixture of two Rare Earth elements, neodymium and praseodymium. It is popular as a visible wavelength standard in the form of doped glass. This Starna liquid reference extends the range of the reference into the UV region, and gives 14 sharp peaks over the range 290 to 870 nm. Absorbance (A) Didymium Wavelengths (nm) 12

13 Far UV Reference (Rare Earth oxide in sulfuric acid) Certified Wavelengths: 201, 212, 223, 240, 253 nm The validation of the wavelength scale in the Far UV is difficult because of the lack of suitable reference materials. This material, developed by Starna, allows wavelength scale validation down to 200 nm. The Rare Earth oxide is dissolved in very dilute sulfuric acid and provides five peaks for wavelength qualification over the range nm. Absorbance (A) Rare Earth Liquid Wavelengths (nm) Samarium Oxide (Samarium oxide in perchloric acid) Certified Wavelengths: 235, 279, 290, 305, 318, 332, 344, 362, 374, 391, 401, 415, 464, 479 nm Samarium oxide is a particularly suitable reference material for checking the wavelength scale of a spectrophotometer over the commonly used range of 200 to 500 nm, as it has peaks throughout this region. Many of the peaks are very narrow, not only providing very accurate location of the peak wavelengths, but a useful indication of the spectral bandwidth of the instrument. Absorbance (A) Samarium Oxide Wavelengths (nm) Suggestions for Use Scan the filter over its usable range and identify the peaks whose wavelength values are given in the calibration certificate, having regard to the bandwidth of the instrument. Compare the measured values to the certificate values. Taking the expanded uncertainty budget of the references into consideration, then the wavelength values should fall within the expected parameters of your instrument if it is working correctly. The results may be used to build up a data log of the instrument s wavelength accuracy over time. This may be used for certification purposes and for troubleshooting should the correlation change. The data will also be useful to a service technician to diagnose and correct any problems that may develop with your instrument. for expanded uncertainty budget and expected parameters see p 37 Holmium Wavelength Reference Cell RM-HL $ Didymium Wavelength Reference Cell RM-DL $ Samarium Wavelength Reference Cell RM-SL $ Far UV Wavelength Reference Cell RM-RE $

14 Stray Light Reference Materials Description & NIST Traceability: Usable Range: Materials with sharp transmission cutoffs at specified wavelengths. Traceable to NIST SRM Complete with UKAS ISO/IEC certificate of validity as an indicator of stray light Detection of instrumental stray light in the UV and visible regions. Measurements made with these references are accepted by the US Pharmacopeia (USP) for instrument qualification. 200 to 390 nm, depending on the material Liquid filters in 10 mm Far UV quartz cells that have been permanently sealed by heat fusion. Supplied with 5 mm cell of the same solution to be used as blank Stray light, also called Stray Radiant Energy or Power, is any light reaching the instrument detector other than that selected by the monochromator. It can be due to optical imperfections or stray reflections within the monochromator itself or to light leaks in the optical system. The detector cannot discriminate between the analytical wavelength and the stray light, so the stray light introduces an error in the measured absorption. The stray light is not absorbed even at high concentrations of the absorbing species, so its effect is a negative deviation from the linear relationship between concentration and absorbance (the Beer-Lambert law) on which most quantitative determinations are based. Stray light is wavelength and instrument dependant. It can be present at any wavelength but is most noticeable when the energy throughput of the system is relatively low, for example in the far UV region. At these wavelengths, any deterioration in the instrument optics or UV light source will exaggerate the apparent stray light. Checking the instrument in the far UV region, even if this is not the area for which it will be primarily used, is an excellent way to monitor the condition of the instrument optics. 5.0 Effects of Stray Light on Instrument Linearity Measured Absorbance (A) Monochromator Stray-light at 5.5A ( %T) level Stray-light at 3.8A (0.02 %T) level Stray-light at 3.5A (0.03 %T) level Stray-light at 3.0A (0.10%T) level Stray-light at 2.8A (0.16 %T) level Stray-light at 2.5A (0.32 %T) level Stray-light at 2A (1.00 %T) level Theoretical Absorbance (A)

15 USP/ASTM Solution Filter Ratio Method NEW USP 857 REFERENCES A range of materials allows stray light to be estimated at different wavelengths: Material Concentration Usable Range Potassium Chloride 1.2% aqueous nm Potassium Iodide 1% aqueous nm Sodium Iodide 1% aqueous nm Acetone Spectroscopy grade nm Sodium Nitrite 5% aqueous nm These reference materials allow you to detect the presence of stray light in your instrument. Each material cuts off all light below a specified wavelength. Any light detected by the instrument below that wavelength must, by definition, be stray light. In this USP approved method of estimating stray light, also known as the Meilenz method, the 10 mm reference material cell is scanned using the 5 mm cell as a blank. The resulting spectrum is in the form of a peak. The peak wavelength will vary according to the optical configuration and stray light characteristics of the instrument under test, but the absorbance value at the peak must exceed 0.7 A to meet the requirements of the USP. This graph shows scans of the 10mm potassium chloride reference cell on three different instruments, using the 5 mm potassium chloride cell as blank. The peak wavelength is different on each instrument due to their different stray light characteristics, but in all three cases the absorbance maximum is greater than 0.7 A, so all three instruments satisfy the USP stray light requirement. Potassium Chloride - Three Spectrophotometer Comparison Differentiial Absorbance (ΔA) Wavelength (nm) The procedure for using the stray light references is similar for all materials. Insert the 10 mm stray light reference cell in the sample beam cell holder and the supplied 5 mm blank cell in the reference beam cell holder of the spectrophotometer. Scan over the stated usable range and note the absorbance at the peak. This absorbance value at the peak must exceed 0.7 A to meet the requirements of USP Chapter <857>. Periodically scan with the same instrument configuration and compare the results. Over time you will have a data trail for your instrument that will make the detection and correction of any problems relating to stray light much more effective. Potassium Chloride, 10mm & 5 mm cells RM-KC/5 $ Potassium Iodide, 10mm and 5 mm cells RM-KI/5 $ Sodium Iodide, 10mm and 5 mm cells RM-SI/5 $ Acetone, 10mm and 5 mm cells RM-AC/5 $ Sodium Nitrite, 10mm and 5 mm cells RM-SN/5 $ Stray Light set Sets can be configured by combining individual $ Stray Light set part numbers: RM-ACKCSI/5 would be Acetone, $ Stray Light set Potassium Chloride and Sodium Iodide $ Note: Potassium Iodide and Sodium Iodide have identical cut-off characteristics and are effectively identical and interchangeable. 15

16 Stray Light Reference Materials (Except USP) Description & NIST Traceability: Usable Range: Materials with sharp transmission cutoffs at specified wavelengths. Traceable to NIST SRM Complete with UKAS ISO/IEC certificate of calibration Detection of instrumental stray light in the UV and Visible regions. Measurements made with these references are accepted by the European Pharmacopoeia for instrument qualification. 200 to 390 nm, depending on the material Liquid filters in far UV quartz cells that have been permanently heat fusion sealed. A blank cell is supplied with each reference or set of references. Stray light, also called Stray Radiant Energy or Power, is any light reaching the instrument detector other than that selected by the monochromator. It can be due to optical imperfections or stray reflections within the monochromator itself or to light leaks in the optical system. The detector cannot discriminate between the analytical wavelength and the stray light, so the stray light introduces an error in the measured absorption. The stray light is not absorbed even at high concentrations of the absorbing species, so its effect is a negative deviation from the linear relationship between concentration and absorbance (the Beer-Lambert law) on which most quantitative determinations are based. Stray light is wavelength and instrument dependant. It can be present at any wavelength but is most noticeable when the energy throughput of the system is relatively low, for example in the Far UV region. At these wavelengths, any deterioration in the instrument optics or UV light source will exaggerate the apparent stray light. Checking the instrument in the far UV region, even if this is not the area for which it will be primarily used, is an excellent way to monitor the condition of the instrument optics. These reference materials allow you to detect the presence of stray light in your instrument. Each material cuts off all light below a specified wavelength. Any light detected by the instrument below that wavelength must, by definition, be stray light. The cut-off wavelength is defined as the wavelength at which the absorbance spectrum transitions through 2 A. Effects of Stray Light on Instrument Linearity 5.0 Measured Absorbance (A) Monochromator Stray-light at 5.5A ( %T) level Stray-light at 3.8A (0.02 %T) level Stray-light at 3.5A (0.03 %T) level Stray-light at 3.0A (0.10%T) level Stray-light at 2.8A (0.16 %T) level Stray-light at 2.5A (0.32 %T) level Stray-light at 2A (1.00 %T) level Theoretical Absorbance (A)

17 ASTM 387 Specified Wavelength Method A range of materials allows stray light to be estimated at different wavelengths: Material Concentration Cut-off Usable Range Potassium Chloride 1.2% aqueous 200 nm nm Sodium Chloride 1% aqueous 205 nm nm Lithium Carbonate Saturated aqueous 227 nm nm Potassium Iodide 1% aqueous 260 nm nm Sodium Iodide 1% aqueous 260 nm nm Acetone Spectroscopy grade 326 nm nm Sodium Nitrite 5% aqueous 391 nm nm Starna stray light CRMs have very sharp transitional (cut-off) spectra, giving excellent filtering characteristics: Stray Light Cut-off Spectra 4.00 Sodium Nitrite 3.50 Acetone Absorbance (A) Sodium Iodide Potassium Iodide Lithium Carbonate 1.00 Potassium Chloride Sodium Chloride Wavelengths (nm) The procedure for using the stray light references is similar for all materials. Set your spectrophotometer s wavelength 20 nm above the cutoff wavelength (for Potassium Iodide you would start at 280 nm). Insert the stray light reference cell in the sample beam cell holder of the spectrophotometer and the supplied blank cell in the reference beam cell holder. Scan down into the UV to the lowest wavelength of the usable range of the reference material. Any light transmitted below the cutoff wavelength will be stray light. If the amount of stray light is greater than the specification given in your instrument manual, a service technician may be required to investigate and correct the problem. Periodically scan with the same instrument configuration and compare the results. Over time you will have a data trail for your instrument that will make the detection and correction of any problems relating to stray light much more reliable. per set Potassium Chloride & Blank Cell RM-KC $ Sodium Chloride & Blank Cell RM-SC $ Lithium Carbonate & Blank Cell RM-LC $ Potassium Iodide & Blank Cell RM -KI $ Sodium Iodide & Blank Cell RM-SI $ Acetone & Blank Cell RM-AC $ Sodium Nitrite & Blank Cell RM-SN $

18 Ultraviolet Bandwidth - Toluene in Hexane NEW USP 857 REFERENCE Description & NIST Traceability: Usable Range: Quartz cell filled with toluene in hexane and permanently sealed by heat fusion, NIST traceable complete with UKAS ISO/IEC accredited certificate of calibration Determination of bandwidth and resolution in the UV region 267 to 270 nm Far UV quartz cells that have been permanently sealed by heat fusion The Spectral Bandwidth (SBW) of a spectrophotometer is the basis of establishing its ability to resolve spectral features separated by small differences in wavelength. If the spectrum of a sample to be measured is suspected of having an interfering peak within the stated spectral bandwidth of the instrument then it should be established whether or not the spectrophotometer is able to resolve the two peaks separately or mixes the two absorption peaks into a single peak, leading to erroneous results. Toluene in hexane is used as a reference for the calculation of SBW. The ratio of the absorbance values of the solution at the peak at nm and the trough at nm relates directly to the SBW of the instrument being assessed. Regular use of this technique will ensure that the resolution of your instrument is within the required range for your work. The toluene in hexane reference consists of two far UV quartz spectrophotometer cells with the solution permanently sealed Absorbance (A) Toluene in Hexane Wavelength (nm) in the cells by heat fusion. One cell contains 0.02% toluene in hexane, the other is a hexane only blank. The Toluene in hexane cell is traceable to SRM 935a which is a transmittance/ absorbance reference material. 269 Table of Approximate Ratios 270 SBW 05 nm SBW 10 nm SBW 15 nm SBW 17 nm SBW 18 nm SBW 20 nm SBW 30 nm SBW: Ratio: Scan the toluene in hexane cell against the hexane blank cell from 265 nm to 270 nm at a bandwidth setting appropriate to your analysis. Calculate the ratio of the absorbance values at the peak at nm and the trough at nm and compare the result with the values in the certificate to determine the spectral bandwidth of your instrument. If the spectral bandwidth is greater than the specification given in your instrument manual, a service technician may be required to investigate and correct the problem. Periodically scan with the same instrument configuration and compare the results. Over time you will have a data trail for your instrument that will make the detection and correction of any problems relating to spectral bandwidth much more reliable. Toluene in Hexane cell with blank RM-TX $

19 Ultraviolet Bandwidth Derivative Spectrophotometry-Toluene in Methanol Description & NIST Traceability: Usable Range: 0.020% v/v solution of toluene in methanol, permanently sealed by heat fusion into a far UV quartz cell. Supplied with a methanol blank. Traceable to NIST SRMs and supplied with UKAS ISO/IEC accredited certificate of calibration. Qualification of UV spectrophotometers for bandwidth (resolution) in derivative spectroscopy 230 to 290 nm Far UV quartz cells that have been permanently sealed by heat fusion This reference is described in the European Pharmacopoeia as a resolution test for use in derivative spectroscopy. In derivative spectroscopy, absorption spectra are transformed into first- second- or higher-order derivatives. This technique facilitates the resolution of two or more peaks that overlap and become merged into a composite curve, and are represented by shoulders on that curve rather than as individual peaks. By calculating the derivative, the underlying individual spectra can be more readily identified. The reference consists of 0.020% v/v solution of toluene in methanol, permanently sealed by heat fusion into a far UV quartz cell and supplied with a methanol blank. The spectrophotometer to be tested must have the ability to produce second-order derivative spectra. The procedure will be described in the instrument s operating manual. Scan the toluene in methanol reference cell in second-derivative mode using the methanol cell as a blank. The derivative spectrum should be similar to that shown, with a small negative extremum ( )between two larger negative extrema at 261 nm and 268 nm. To comply with the requirements of the European Pharmacopoeia, the ratio A/B should be not less than 0.2. If the test fails, a service technician may be required to investigate and correct the problem. In any case, repeating the test at intervals will allow the creation of an audit trail that will assist with certification and the rectification of any instrument problems % v/v Toluene in Methanol Toluene in Methanol Resolution - 2 nd Order Derivative scan Absorbance (A) Second Derivative Units 2nd Order Derivative (d"a) A Wavelength (nm) B Absorbance 2nd Derivative Wavelength (nm) Toluene in Methanol cell with blank RM-TM $

20 Ultraviolet Resolution Benzene Vapor Description: Usable Range: 0.01 ml Benzene in a vapor state sealed in a quartz cell Test the resolution at various bandwidths of grating based instruments in the UV 240 to 265 nm Far UV quartz cell that has been permanently sealed by heat fusion The ability of your spectrophotometer to resolve absorption peaks is the basis of its theory of operation. If absorption peaks cannot be resolved adequately, then interfering peaks will be integrated with the correct peaks, thus giving an inaccurate measurement. Benzene vapor is an effective material for the detection of a grating based spectrophotometer s resolving power, as it has a great number of close but distinct absorption peaks. Benzene vapor may not work well with a photodiode array spectrophotometer. Benzene Vapor Spectra at various Spectral Bandwidths (SBWs) - offset for clarity Absorbance (A) nm SBW 0.2 nm SBW 0.5 nm SBW 1.0 nm SBW Wavelength (nm) Set your spectrophotometer to a Spectral Bandwidth (SBW) and a scan rate and note the settings. Scan from 240 to 265 nm and compare the peaks to the two charts and determine what changes need to be made to the setting of your spectrophotometer to improve the resolution of the scan. Continue to alter the bandwidth setting until you have optimized the resolution. Make a note of the settings in your quality procedures. Each time that the scan is repeated you can compare the new scan with previous scans to check for any variance. If your instrument needs service because of a drop in resolution, the historical data will greatly assist the service technician. Description Part Number Price Instrument Resolution reference cell, Benzene Vapor RM-BZ $

21 UV-Visible Wavelength - Holmium & Didymium Glass Description & Traceability: Usable Range: Holmium glass filter and Didymium glass filter, NIST traceable complete with UKAS ISO/IEC accredited certificate of calibration Verification of wavelength scale accuracy in both UV and visible 241 nm to 640 nm (Holmium). 430 nm to 890 nm (Didymium) Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.50, 1, 1.5, 2, and 3 nm. Other SBWs can be provided on request. Stress free glass filter mounted in an anodized aluminum holder Both Holmium and Didymium provide distinctive peaks that make each suitable for use as a wavelength standard. These robust Starna filters are more suitable for some environments than the liquid cells. They are mounted in an anodized aluminum housing to protect the polished surfaces. The Holmium and Didymium glass filters are used to ensure that the instrument wavelength scale is within the expected tolerances for the actual wavelength being measured. Absorbance (A) Holmium Glass Wavelength (nm) Didymium Glass Absorbance (A) Holmium and Didymium filters present a wide range of peaks that can be resolved. The number of peaks resolved will depend on the spectral bandwidth used. The wavelength peaks can then be used to compare the wavelength indicated by your spectrophotometer to the known peaks. The initial procedure should be to scan the filter over the usable range to identify all the peaks that can be resolved at the specified SBW of the instrument. Adjust the Spectral Bandwidth and scan rate to produce a usable spectra. Check each peak to ensure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, a service technician may be required to check or adjust your instrument. The filters should be measured against air. Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Holmium or Didymium reference will enable the user to build a data log of the instrument s spectral accuracy for use with certification and troubleshooting should the correlation change over time. Holmium Glass, Wavelength reference filter, NIST traceable RM-HG $ Didymium Glass, Wavelength reference filter, NIST traceable RM-DG $

22 Neutral Density Filters - Visible Description & NIST Traceability: Certified Wavelengths: Neutral density glass filters calibrated for absorbance/transmission in the visible region of the spectrum. Traceable to NIST SRMs and supplied with UKAS ISO/IEC accredited certificate of calibration. Validating the photometric accuracy and linearity of spectrophotometers in the visible region , 465.0, 546.1, 590.0, nm Glass filters mounted stress free in anodized aluminum holder 22 These filters are traceable to NIST SRMs, including sets SRM 930e, SRM 1930 and SRM 2930 They are used to validate the photometric scales of visible spectrophotometers in both transmission and absorbance. Manufactured from Schott NG type glass, the thickness and glass type are calibrated to produce filters with known absorbance and transmittance values. The filters are mounted stress free in a black anodized aluminum holder compatible with all standard spectrophotometer cell holders. Each NIST traceable filter is individually tested and certified. The spectrum of these filters is relatively flat over most of the range of calibration. Zero the instrument using the empty filter holder supplied and measure the filters at the certified wavelengths. Compare the measured values to the certificate values. Taking the expanded uncertainty budget of the references into consideration, then the absorbance values should fall within the expected parameters of your instrument if it is working correctly. If more than two filters with different absorbance values are available, the values may be used to plot a graph to establish the instrument s linearity. NIST Compliance NIST lists three specifications for neutral density filter Standard Reference Materials (SRMs): SRM 930e, SRM 1930 and SRM These specifications also stipulate the maximum spectral bandwidth to be used at these wavelengths for certification measurements: For those users wishing to work to these specifications, sets of filters equivalent to these SRMs are available These sets consist of three filters together with an empty aluminum holder, to be used as a blank. These SRMs were originally specified in transmission, the specified nominal transmission and approximate absorbance values are shown in the table: filters cover absorbance values from 0.04 A to 3.5 A (92 %T to 0.03 %T.) They are available individually or in convenient sets see opposite page for details. An empty filter holder is supplied as a measurement blank. The results may be used to build up a data log of the instrument s photometric accuracy over time. This may be used for certification purposes and for troubleshooting should the correlation change. The data will also be useful to a service technician to diagnose and correct any problems that may develop with your instrument. for expanded uncertainty budget and expected parameters see p 37 Wavelength (nm) SBW (nm) SRM 930e SRM 1930 SRM 2930 %T A %T A %T A Filter Filter Filter For convenience, all certification measurements are made at a SBW of 1.0 nm. This complies fully with the SRM specifications. Actual certificate values are given in both Transmission (%T) and Absorbance (A). Absorbance (A) Neutral Density Filters Wavelengths (nm) D1 D3 N1 N3 1N 2N 3N 5N 9N

23 NEW USP 857 REFERENCE NIST SRM Equivalent Sets, referenced in USP <857>: SRM 930e Set: 10, 20 & 30 %T Neutral Density Filters and blank holder RM-1N2N3N $ SRM 1930 Set: 1, 3 & 50 %T Neutral Density Filters and blank holder RM-N1N35N $ SRM 2930 Set: 0.1, 0.3 & 92 %T Neutral Density Filters and blank holder RM-D1D39N $ Combined SRM Set: All 9 Neutral Density Filters listed above and blank holder RM-SRM9ND $ Transmittance/Absorbance and Wavelength Set (USP <857> compliant): This set combines three neutral density filters (with blank holder) and a holmium glass filter and is a convenient way of qualifying a visible spectrophotometer for photometric, linearity and wavelength accuracy. Approximate values are: Neutral Density Filters 1.00, 0.50 & 0.25 A (10, 30 & 52 %T) Holmium Glass Filter (see page 21) 11 peaks from 240 nm to 640 nm. Description Part Number, NIST Traceable Price Neutral Density and Holmium Filter Set RM-1N3N5DHG $ Starna Neutral Density Filters Available individually or as sets of 4, 6 and 9 filters. The Catalogue numbers and contents of these kits are given in the table below: % Transmittance Absorbance Single filter Set of 4 filters RM-4ND Part Number Set of 6 filters RM-6ND Set of 9 filters RM-9ND Set of 9 filters RM-SRM9ND RM-9N RM-8N RM-7N RM-6N RM-5D RM-5N RM-3N RM-2D RM-2N RM-1N RM-N RM-N RM-1D RM-N RM-D RM-D RM-H RM-H3 Price $ $ $ $ $ Note: Any choice of filters can be combined in a set simply by combining the individual part numbers. For example RM-1N2N3N is a set of 3 filters with transmittance values 10, 20 and 30% T. 23

24 Microplate Reader Visible Reference Plate Description: Certified Wavelengths: 96 well microplate validation filters for wavelength and absorbance scales, NIST Traceable complete with UKAS ISO/IEC accredited certificate of calibration Validation of the visible photometric and wavelength scale of 96 well microplate readers. Wavelength and Holmium - 360, 418, 445, 453, 460, 536, 637 nm, Neutral Density , 465.0, 546.1, 590.0, nm Glass Filters mounted in 96 well format anodized aluminum holder This microplate is used to test the photometric scale and wavelength accuracy of 96 well plate readers in the visible wavelength range. The absorbance filters are made from Schott NG-type glass, which absorbs a known percentage of the light passing through it. They are based on the NIST sets SRM 930e, SRM 1930 and SRM The thickness of the particular glass type used is calculated to produce filters of approximate transmittance/ absorbance values; the approximate values are shown below. Each NIST traceable filter is individually tested and certified. The Holmium Glass filter has peaks from 360 nm to 637 nm. Specifications: Certified Wavelengths: Neutral Density Absorbance Filters: nm nm nm nm nm Holmium Wavelength Filter (approximate wavelengths): 637 nm 536 nm 460 nm 453 nm 445 nm 418 nm 360 nm Filters in reference plate: Configuration of Reference Plate: Well Positions Filter Type Filter Values C2 - H2 Air Blank C4 - H4 Neutral Density 30%T (0.5 Absorbance) C6 - H6 Neutral Density 10%T (1.0 Absorbance) C8 - H8 Neutral Density 3%T (1.5 Absorbance) C10 - H10 Neutral Density 1%T (2.0 Absorbance) C12 - H12 Holmium Peaks from 360 to 637 nm 24 per set 96 Well Microplate Reader Validation Plate RMMR-1N3NN1N3HG $

25 Starna References to 96 Well Microplate Adaptor Description: Usable References: Usable Cells Physical Appearance: Compatible Readers: A patented adaptor plate (Patent Number US ), which allows the use of either Starna glass or liquid references in the validation of a 96 well microplate reader. Performance validation of your microplate reader with Starna Reference sets All standard references and filters produced by Starna. Specially designed screw-top cells manufactured by Starna to fit the adaptor plate. These may be used with any solution for analysis. Injection molded polymer tray with slots for 8 references or cuvettes The adaptor is compatible with all 96 well plate readers Hole alignment of the CuvettePlate conforms to the international standard and precise location of the wells in a 96 well microplate. Slots precisely align the cuvettes over the available holes through the bottom plate. A small ridge in the center of the CuvettePlate slightly tilts the cuvettes so that the bubble in a liquid sample does not interfere with the light beam of the microplate reader. Validating your microplate reader with liquid filled cuvettes is easy with the CuvettePlate. Place your NIST traceable references in the CuvettePlate and they will be aligned into the format for your reader and held so that the bubble in the reference cell will not interfere with the analysis. You can use any of the Starna validation references as well as the NIST SRM-2034 Holmium. Microplate readers can be validated using exactly the same NIST traceable filters either liquid or for instance Neutral Density Filters such as our Part Number RM-1N2N3N or NIST SRM-930), that you may already be using to calibrate other spectrophotometers in your laboratory. Under these circumstances it is not necessary to purchase extra reference materials. This also allows laboratories to crossvalidate and make comparisons between different types of spectrophotometer instruments. Description Part Number Price CuvettePlate, 96 well microplate adaptor SCP-96-8R $ mm Quartz Cell with PTFE screw-cap 1.30-Q-10-ST $

26 260/280 nm Validation Reference for DNA and RNA Description: Certified Wavelengths: Standard solution which gives a stable 260/280 nm ratio of 1.8 to 2.0, NIST Traceable complete with UKAS ISO/IEC accredited certificate of calibration Control the quality of DNA/RNA purity analysis 260 nm and 280 nm Far UV quartz cell that has been permanently sealed by heat fusion. The reference is also available in vials containing 1.5 ml, with a screw cap. When running DNA or RNA purity analysis on a spectrophotometer, it is important to ensure that the instrument is working correctly. The Starna DNA/RNA validation reference (DNACON) is a stable solution, which mimics the 260/280 nm ratio of DNA and RNA. The RM-DNA (permanently sealed reference) is supplied with a certificate which lists the expected 260/280 nm ratio and the confidence limit of the ratio. The validation analysis is performed by our ISO/IEC accredited laboratory and is traceable to NIST. The screw cap vials reseal to maintain the integrity of the DNACON. This format allows for the removal of the DNACON by pipette or syringe for use in microvolume devices. The vials are available in concentrations of 5x and 10x as listed below. RM-DNA Screw Cap DNACON 1.5 ml Vials Typical Uncorrected Absorbance Values: nm A 280 nm A Ratio: 1.89

27 DNACON Solution Absorbance (A) , Wavelengths (nm) The importance of this reference is that it allows the user to validate an instrument's performance in real time. When measuring unknown samples the user can verify that the instrument is working correctly by reading the validation standard both before, during and after each run of unknowns. When using a spectrophotometer which automatically calculates the 260/280 ratio, place the DNA/RNA validation reference in the spectrophotometer and run the instrument's routine for the 260/280 ratio. If the ratio is valid when compared to the certificate then the user can be assured that the instrument is calculating the ratio correctly. In a spectrophotometer without an automatic calculation feature, simply use the DNA/RNA validation reference as a normal sample to validate your analytical procedure. With the vial references, the 5x concentration is suitable for the NanoDrop instrument while the 10x concentration is suitable for micro drop systems such as Jenway/Bibby. Additionally, at 260 nm, results are approximately as follows: Path length (mm) 5X Concentration 10X Concentration A 0.2 A A 0.5 A A 1 A 10 5 A 10 A NanoDrop is a trade mark of Thermo Fisher Scientific Inc DNACON reference, 10 mm cell RM-DNA $ X concentration, 1 Screw Cap Vial DNACON5X-SC1 $ X concentration, 2 Screw Cap Vials DNACON5X-SC2 $ X concentration, 4 Screw Cap Vials DNACON5X-SC4 $ X concentration, 1 Screw Cap Vial DNACON10X-SC1 $ X concentration, 2 Screw Cap Vials DNACON10X-SC2 $ X concentration, 4 Screw Cap Vials DNACON10X-SC4 $

28 Neutral Density Filters - Near Infra Red Description & Traceability: Certified Wavelengths: Neutral Density Glass Filters for use in the NIR region of the spectrum. Traceable to NRC (Canada) primary references and supplied complete with UKAS/IEC certificate of calibration Validation of the photometric scale of near infrared spectrophotometers 1100, 1700, 2210, 2500 & 2850 nm Glass filters mounted stress-free in anodized aluminum holder Absorbance (A) R1 R3 I1 The filters are traceable to Primary references calibrated by NRC (National Research Council) of Canada, with accreditation to ISO/IEC and ISO Guide 34, and approval through the APLAC/ILAC Mutual Recognition Agreement (MRA). They can be used to validate the photometric scale of near infrared spectrophotometers. Manufactured from Schott NG type glass, the thickness and glass type are calibrated to produce filters with known absorbance and transmittance values. The filters are mounted stress-free in a black anodized aluminum holder compatible with all standard spectrophotometer cell holders. The spectrum of these filters is relatively flat over most of the range of calibration, but with significant deviation above 2700 nm. Approximate values are given below: actual values are given in the certificate supplied with the filters. Wavelength 1100nm 1700nm 2210 nm 2500 nm 2850 nm Scale %T A %T A %T A %T A %T A R R I Zero the instrument using the empty filter holder supplied and measure the filters at the certified wavelengths. Compare the measured values to the certificate values. Taking the expanded uncertainty budget of the references into consideration, then the absorbance values should fall within the expected parameters of your instrument if it is working correctly. If more than two different filters are available, the values may be used to plot a linearity graph. The results may be used to build up a data log of the instrument s photometric accuracy over time. This may be used for certification purposes and for troubleshooting should the correlation change. The data will also be useful to a service technician to diagnose and correct any problems that may develop with your instrument. for expanded uncertainty budget and expected parameters see p Description Part Number, NRC (Canada) Traceable Price Neutral Density Filter, nm & Blank holder RM-R1 $ Neutral Density Filter, nm & Blank holder RM-R3 $ Neutral Density Filter, nm & Blank holder RM-I1 $ Neutral Density Filter set, 20, 25 & nm & Blank holder RM-R1R3I1 $

29 Metal on Quartz Near Infra Red (NIR) Absorbance/Linearity Description & Traceability: Certified Wavelengths: ISO Accredited: Metal on Quartz filter for absorbance/linearity in the NIR area of the spectrum. NIST/NRC (Canada)Traceable, complete with UKAS ISO/IEC accredited certificate of calibration 250.0, 360.0, 465.0, 546.1, 635.0, 1100, 1700, 2210, 2500, 2800 nm Metal coated quartz filter with coated side protected by an optically contacted quartz window. The filters are mounted in protective aluminum holders that will fit a standard instrument cell holder. ISO/IEC 17025, ISO Guide 34 by UKAS & ISO 9001 by BSI. A standard range of Metal on Quartz absorbance standards for the NIR area of the spectrum to facilitate full compliance with USP requirements. All Starna Metal on Quartz Filters have an optically contacted quartz cover plate to protect the metalized surface and facilitate easy cleaning. RM-1NQ3NQ9NQ The filters allow absorbance and linearity at certified wavelengths across the range from 250 nm to 2800 nm and the relative values are shown on the table to the right. They can be certified at wavelengths other than those shown above, on request. The filters are produced in our ISO 9001 accredited production facility under ISO Guide 34 as a reference material producer and calibrated in our ISO/IEC accredited calibration laboratory. The NIR Metal on Quartz Filters are intended for use across a broad spectrum including the NIR with a relatively flat transmission profile across all wavelengths. Normally three representative absorption filters would be chosen for any given application but, the filters may be bought individually, in sets as shown below, or any combination from the standard range. Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty for expanded uncertainty budget and expected parameters see p Metal on Quartz NIR Filters: Part No. %T Transmittance Absorbance NQ1 1% 2.00 A NQ3 3% 1.52 A 1NQ 10% 1.00 A 3NQ 30% 0.52 A 5NQ 50% 0.30 A 9NQ 90% 0.06 A budget of the certified references into consideration, if the absorbance values fall within the expected parameters of the instrument then the instrument is working correctly. It is important to confirm that Metal on Quartz filters are suitable for use with the instrument under test as they have a highly reflective surface and some instruments are sensitive to the positioning of such materials in the sample holder. Description Part Number, NIST/NRC (Canada) Traceable Price Metal on Quartz NIR Reference, 1% & Blank Holder RM-NQ1 $ Metal on Quartz NIR Reference, 3% & Blank Holder RM-NQ3 $ Metal on Quartz NIR Reference, 10% & Blank Holder RM-1NQ $ Metal on Quartz NIR Reference, 30% & Blank Holder RM-3NQ $ Metal on Quartz NIR Reference, 50% & Blank Holder RM-5NQ $ Metal on Quartz NIR Reference, 90% & Blank Holder RM-9NQ $ Metal on Quartz NIR Reference, 3%, 50% & Blank Holder RM-NQ35NQ $ Metal on Quartz NIR Reference, 10%, 30%, 90% & Blank Holder RM-1NQ3NQ9NQ $ Metal on Quartz NIR Reference, 1%, 3%, 50%, Blank Holder RM-NQ1NQ35NQ $ Transmission (%T) NIR Metal-on-Quartz filters Wavelength (nm) 9NQ 5NQ 3NQ 1NQ 29

30 Near Infrared - Wavelength Reference Description & NIST Traceability: Quartz cell in either Transmittance or Transmittance/Transflectance format. Traceable to NIST SRM 2065, complete with UKAS ISO/IEC accredited certificate of calibration Verification of wavelength scale accuracy in the NIR spectrum Certified Wavelengths: 990.7, , , , , , , , , , , , nm Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2 and 3 nm. Other SBWs can be provided on request. Filled near IR quartz cell that has been permanently sealed by heat fusion The reference is used to validate the wavelength scale of a NIR spectrophotometer. With 13 certified peaks from 990 to 2537 nm that include some complex peaks, which may be used for instrument resolution evaluation. The reference is available in two formats: Transmittance: - 10 mm optical pathlength with two clear windows (opposite windows to allow light to pass through the cell). Transmittance/Transflectance - 5mm or 10mm optical path length, depending on orientation. In 5 mm configuration, the rear window is mirror coated to provide reflectance optical return so pathlength is effectively 10 mm ( 2 x 5 mm). Each reference set is supplied with full certification and traceability to NIST through SRM The reference material is produced and certified in our ISO Guide 34 and ISO/IEC accredited laboratory. The reference can also be recertified as required. Transmittance Transmittance/ Transflectance Resolvable Peaks nm nm nm nm nm nm nm nm nm nm nm nm nm Absorbance (A) Wavelengths (nm) Scan the cell over its usable range and identify the peaks whose wavelength values are given in the calibration certificate, having regard to the bandwidth of the instrument. Compare the measured values to the certificate values. Taking the expanded uncertainty budget of the references into consideration, then the wavelength values should fall within the expected parameters of your instrument if it is working correctly. The results may be used to build up a data log of the instrument s wavelength accuracy over time. This may be used for certification purposes and for troubleshooting should the correlation change. The data will also be useful to a service technician to diagnose and correct any problems that may develop with your instrument. for expanded uncertainty budget and expected parameters see p NIR Wavelength Reference, Transmittance RM-NIR $ NIR Wavelength Reference, Transmittance and Transflectance RM-NIR/T $

31 Chloroform NIR Stray Light Reference Description and NIST Traceability: Usable Range: Material with sharp transmission cut - off (i.e. absorbance > 2.0 A) at approximately 2365 nm. Traceable to NIST SRM 2065, complete with UKAS ISO/IEC Certificate of Calibration. Detection of instrumental stray light in the near infrared region to 2385 nm Liquid filter in 10 mm near IR quartz cell that has been permanently sealed by heat fusion. Stray light, also called Stray Radiant Energy or Power, is any light reaching the instrument detector other than that selected by the monochromator. It can be due to optical imperfections or stray reflections within the monochromator itself or to light leaks in the optical system. The detector cannot discriminate between the analytical wavelength and the stray light, so the stray light introduces an error in the measured absorption. Its effect is a negative deviation from the linear relationship between concentration and absorbance (the Beer-Lambert Law) on which most quantitative determinations are based. Measured Absorbance (A) Effects of Stray Light on Instrument Linearity Theoretical Absorbance (A) Monochromator Stray-light at 5.5A ( %T) level Stray-light at 3.8A (0.02 %T) level Stray-light at 3.5A (0.03 %T) level Stray-light at 3.0A (0.10%T) level Stray-light at 2.8A (0.16 %T) level Stray-light at 2.5A (0.32 %T) level Stray-light at 2A (1.00 %T) level Stray light is wavelength and instrument dependant. It can be present at any wavelength but is most noticeable when the energy throughput of the system is relatively low, when any deterioration in the instrument optics or light source will exaggerate the apparent stray light. Regular stray light checks are an excellent way of monitoring the condition of the instrument optics Chloroform - NIR Stray Light This reference material consists of spectroscopic grade chloroform, permanently sealed by heat fusion in a high quality near IR quartz cell. Sealing the cell by this method eliminates the risks associated with handling this volatile, hazardous substance. The spectrum shows a cut - off (i.e. Absorbance > 2.0 A) at approximately 2365 nm. Any light detected within ± 20 nm of this wavelength will be stray light. Absorbance (A) Wavelengths (nm) Scan the chloroform reference over a range ± 20 nm either side of the certified cutoff wavelength. The indicated absorbance at the certified wavelength should be < 2 A. Note: This assumes that the measurement has been performed on a NIR spectrophotometer with appropriate resolution, i.e. at the spectral bandwidth set on the instrument the wavelength distribution at 2365 nm does not exceed ± 20 nm. If this does occur, the stray light will be over-estimated. Periodically scan with the same instrument configuration and compare the results. Over time you will have a data trail for your instrument that will make the detection and correction of any problems relating to stray light much more effective. per set Chloroform Stray Light Cell RM-CHCL3 $

32 Mid Infrared Wavelength Reference Polystyrene Description and Traceability: Usable Range: Wavenumber Resolution: 38 µm polystyrene film in a card holder. Traceable to NIST SRM 1921b. Supplied complete with UKAS ISO/IEC accredited certificate of calibration. Qualification of the wavelength scale of mid infrared spectrophotometers. 540 cm -1 to 3125 cm -1 (3.2 µm to 18.5 µm). 2 cm -1 or better Certified Wavelengths: 539, 842, 907, 1028, 1069, 1155, 1583,1601, 1943, 2849, 3001, 3026, 3060, and 3082 cm -1 Polystyrene film mounted in card holder compatible with the sample holders of most infrared spectrophotometers Polystyrene film has been a popular wavelength reference for mid-infrared spectrometers for many years. These new NIST-traceable Starna Reference Materials can be used to qualify Mid IR - FTIR spectrophotometers for wavelength accuracy over the range 3.2 µm to 18.5 µm (3125 cm -1 to 540 cm -1 ). Transmittance (%T) Polystyrene Infrared (IR) Spectrum SRM 1921b Starna 38µm Traceable to NIST SRM 1921b, 14 certified peaks are available for wavelength qualification purposes in the mid infrared. Approximate peak wavelength values are: Wavenumber (cm-1) , 842, 907, 1028, 1069, 1155, 1583,1601, 1943, 2849, 3001, 3026, 3060, and 3082 cm -1. Actual values are given in the calibration certificate supplied with the reference. The instrument to be qualified should have a wavenumber resolution of 2 cm -1 or better. Scan the filter over its usable range and identify the peaks whose wavelength values are given in the calibration certificate. Compare the measured values to the certificate values. Taking the expanded uncertainty budget of the references into consideration, then the wavelength values should fall within the expected parameters of your instrument if it is working correctly. The results may be used to build up a data log of the instrument s wavelength accuracy over time. This may be used for certification purposes and for troubleshooting should the correlation change. The data will also be useful to a service technician to diagnose and correct any problems that may develop with your instrument. for expanded uncertainty budget and expected parameters see p 37 per set 32 Mid IR-FTIR Polystyrene Reference, NIST Traceable RM-1921/38 $

33 Mid & Near Infrared Wavelength Reference Polystyrene Description and Traceability: Usable Range: Wavenumber Resolution: 65 µm polystyrene film in a card holder. Traceable to NIST SRM 1921b and NIST SRM Supplied complete with UKAS ISO/IEC accredited certificate of calibration. Qualification of the wavelength scale of mid- and near infrared FTIR spectrophotometers. 540 cm -1 to 9000 cm -1 (1.1 µm to 18.5 µm). 2 cm -1 or better Certified Wavelengths: 539, 842, 907, 1028, 1069, 1155, 1583,1601, 1943, 2849, 3001, 3026, 3060, and 3082 cm -1 (Mid IR); 3064, 3647, 4039, 4250, 4335, 4608, 5952, 8749 cm -1 (Near IR) Polystyrene film mounted in card holder compatible with the sample holders of most infrared spectrophotometers b m Polystyrene film has been a popular wavelength reference for infrared spectrometers for many years. These new NIST-traceable Starna Reference Materials can be used to qualify Mid IR and Near IR-FTIR spectrophotometers for wavelength accuracy over the range 540 cm -1 to 9000 cm -1 (1.1 µm to 18.5 µm). 14 certified peaks, traceable to NIST SRM 1921b, are available for wavelength qualification purposes in the mid infrared. Approximate peak wavelength values are: 539, 842, 907, 1028, 1069, 1155, 1583,1601, 1943, 2849, 3001, 3026, 3060, and 3082 cm -1. (see spectrum on p 32) In addition, eight peaks, traceable to NIST SRM 2065, can be used for wavelength qualification in the near infrared. Approximate peak wavelength values are: cm nm Transmittance (% T) Polystyrene NIR Spectrum Wavenumber (cm-1) The instrument to be qualified should have a wavenumber resolution of 2 cm -1 or better. Scan the filter over its usable range and identify the peaks whose wavelength values are given in the calibration certificate. Compare the measured values to the certificate values. Taking the expanded uncertainty budget of the references into consideration, then the wavelength values should fall within the expected parameters of your instrument if it is working correctly. The results may be used to build up a data log of the instrument s wavelength accuracy over time. This may be used for certification purposes and for troubleshooting should the correlation change. The data will also be useful to a service technician to diagnose and correct any problems that may develop with your instrument. for expanded uncertainty budget and expected parameters see p 37 per set Mid IR-FTIR/NIR Polystyrene Reference, NIST Traceable RM-1921/65 $

34 High Purity Water Fluorescence Sensitivity Description: Usable Range: ISO/IEC Accreditation: Ultra-high pure water, permanently sealed by heat fusion in a high quality quartz fluorometer cell. Evaluation of sensitivity in the qualification of fluorescence spectrophotometers specifically the instrument signal-to-noise ratio near the detection limits using the Raman band emission at specific wavelengths. 250 to 600 nm Far UV quartz fluorescence cell that has been permanently sealed by heat fusion. Produced and manufactured under ISO/IEC 17025, ISO Guide 34 by UKAS protocols When excited at appropriate wavelengths, pure water exhibits a Raman shift spectrum analogous to fluorescence. This spectrum can be used to evaluate the signal-tonoise ratio of fluorescence spectrophotometers near the detection limit. The signal to-noise ratio for a given excitation wavelength may be calculated using the instrument software. Note that the calculated ratio will depend on the calculation protocol employed by the instrument manufacturer and therefore 'compliance to specification' limits will be specific to the spectrofluorometer type under test. This reference with a Certificate of Validation from the Starna Calibration Laboratory which is supplied accredited by UKAS under ISO 9001, ISO/IEC 17025, and ISO Guide 34. Molecular Emission Spectrum Fluorescence Sensitivity Water Raman peak Excitation: 350 nm Emission peak: 398 nm Molecular Emission Spectrum Fluorescence Sensitivity Water Raman peak Excitation: 500 nm Emission peak: nm Relative Intensity Units (RIU) Relative Intensity Units (RIU) Wavelength (nm) Wavelength (nm) Record the emission spectrum of the water reference using excitation wavelengths of 350 nm or 500 nm depending on the wavelength range of interest. By reference to the appropriate section of the instrument control software, calculate the signal-tonoise ratio for a given excitation wavelength and compare it with the instrument specification. Description Part Number Price 34 Ultra-pure Water Reference RM-H2O $

35 Quinine Sulfate Fluorescence Reference Description & NIST Traceability: Usable Range: Solution of quinine sulfate in mol/l perchloric acid. The reference material is permanently sealed by heat fusion in quartz fluorometer cells, NIST Traceable complete with UKAS ISO/IEC accredited certificate of calibration. Calibration and evaluation of methods used on a fluorescence spectrophotometer. 375 nm to 675 nm Far UV quartz fluorescence cell that has been permanently sealed by heat fusion. The reference is also available with a blank if required. NIST SRM 936a is supplied as a crystalline material and is made into a solution as described in the Certificate of Analysis supplied with NIST SRM 936a. Starna then fills a high quality far UV quartz fluorometer cell with the SRM solution and permanently seals the cell by heat fusion alone. The reference is designed for multiple use over time and for inter-instrument comparisons. Quinine sulfate references can also be supplied with a perchloric acid blank. Where instrument linearity needs to be established, sets with different concentrations of quinine sulfate and a blank are available in the following concentrations, 0.25, 0.5, 0.75 & 1.0 mg/l. The Starna reference set is supplied with a Certificate of Validation by our calibration laboratory which is accredited by UKAS to ISO 9001, ISO/IEC 17025, and ISO Guide 34. The certificate is valid for a period of two years. RM-QS Corrected molecular emission spectrum Quinine Sulfate Quinine Sulfate Linearity Absorbance (A) Relative Intensity Units (RIU) Linearity Regression Coefficient Wavelengths (nm) Quinine Sulfate (mg/l) Record the emission spectrum of the quinine sulfate reference using an excitation wavelength of nm and use the results to calculate the correction factor for your instrument at the wavelength of interest by using the formula and reference values given in the calibration certificate. To evaluate instrument linearity, plot a graph of concentration against emission at 450 nm for each quinine sulfate concentration. Quinine Sulfate Reference (1 concentration 1.0 mg/l, without blank) RM-QS $ Quinine Sulfate Reference (1 concentration 1.0 mg/l & blank) RM-QS00 $ Quinine Sulfate Reference (2 concentrations, & blank) RM-2QS00 $ Quinine Sulfate Reference (3 concentrations, & blank) RM-3QS00 $ Quinine Sulfate Reference (4 concentrations, & blank) RM-4QS00 $

36 Cyclohexane Raman Spectroscopy Wavelength Reference Description: Usable Range: Cyclohexane may be used to qualify the wavelength scale of Raman Spectrometers as described in ASTM E (2014). Complete with ISO Guide 34 certificate of validation Wavelength qualification of Raman spectrometers 380 cm -1 to 3000 cm -1 when using an excitation wavelength of nm. Certified Values: 384.1, 426.3, 801.3, , , , , , , , cm -1 High purity cyclohexane in 10 mm far UV quartz cell that has been permanently sealed by heat fusion. Compared to atomic emission lines, Raman shift standards offer an easier-to-use alternative for qualifying the wavelength scale of Raman Spectrometers. They are also independent of laser frequency. The Raman shift values for cyclohexane have been accurately measured and it is widely used as a reference material. Intensity High-purity Cyclohexane Using an excitation wavelength of nm, 11 shift values covering a range from 384 to 2938 cm -1 can be identified. Values (in cm -1 ) are: Raman Shift (cm -1 ) 384.1, 426.3, 801.3, , , , , , , , The permanently sealed cell eliminates the hazards associated with handling this volatile, toxic and highly flammable material. Determine the Raman shift spectrum of the reference and identify the peaks whose wavelength values are given in the calibration certificate. Compare the measured values to the certificate values. Taking the expanded uncertainty budget of the references into consideration, then the wavelength values should fall within the expected parameters of your instrument if it is working correctly. for expanded uncertainty budget and expected parameters see p 37 per set 36 Cyclohexane Cell RM-C6H12 $

37 Calibration Services Calibration Production Experience & Accreditation History Starna has over forty-five years experience in the production of both glass filters and permanently sealed by heat fusion liquid reference materials. Calibration accreditation through UKAS to ISO/IEC17025 was achieved in This was followed in 2006 by Starna also achieving ISO/IEC Guide 34 as a Reference Material Producer. Calibration is carried out on any one of five fully qualified reference spectrophotometers. The above accreditation, experience and accumulated data puts Starna in a unique position with regard to certified reference materials ranging from the Deep UV to the NIR The scope detailing specifically what the Starna accreditation covers, including uncertainty values, can be viewed at the following URL on the UKAS web site. The above indicated links to the Starna Scientific accreditation scopes are provided so that customers may view the latest accreditation information. Customers are advised that they should always check that the scope of any supplier accreditation includes the references they intend to purchase. References not included in the published scope may not be recognized for instrument qualification purposes by regulatory bodies. Expected Parameters and Expanded Uncertainty Budgets The expanded uncertainty budget (k=2) is the tolerance that we could expect to measure in the assigned value on any one of our reference spectrophotometers during the 2 year period of certification. When used to verify the performance of any given spectrophotometer this certificate tolerance must be added in a simple linear manner to the stated appropriate specification accuracy of the instrument on the test, see following example: Wavelength Absorbance Certificate ±0.10 nm ± A Instrument ±0.30 nm ± A Total ±0.40 nm ± A Certification Provided in USB Electronic Format As a result of increased customer requests for certificates and associated data to be supplied in electronic format, especially from those customers working in controlled environments, in 2013 Starna changed certificates to an electronic format. This advancement in presentation has helped the efficiency of pharmaceutical companies, instrument manufacturers and other customers worldwide, another example of Starna taking the lead in reference material presentation. This environmentally friendly approach allows greater customer flexibility in the use and circulation of calibration certificates, allowing data to be input directly into other databases. For customers for whose protocols do not allow the transfer of data by USB stick, printed/soft copy can be provided at no additional cost on request. Calibration Services The Starna Calibration and Recalibration Laboratory offers a calibration and re-calibration service to ISO/IEC 17025, which is the highest level of accreditation currently available. This calibration service is not only available for reference materials manufactured by Starna, but also for similar materials manufactured by third parties, such as NIST and other commercial organizations. Starna is also able to extend the service to cover a wide range of optical products within the UV-Visible-NIR range. We welcome enquiries for all types of similar product, even though in some instances they may not have been produced in the first instance as certified references. Among the many products that Starna Scientific certify are a wide variety of reference plates for plate reader instruments from a number of manufacturers. Starna normally completes the certification of all references within a period of five working days from receipt of the references at the Starna Calibration Laboratory. In urgent situations completion time may be reduced. Transportation time to and from the calibration laboratory should be allowed for to minimize disruption to continuity of compliance. Regulated laboratories are encouraged to hold two sets of references, purchased twelve months apart. This ensures total continuity of compliance whilst references are returned for revalidation. It also acts as an insurance against accidental breakage. 37

38 Tools for Easier Compliance In a regulated environment, the responsibility of proof is on the user to justify and demonstrate, by the qualification of the system, that a spectrophotometer is fit for purpose and capable of providing the required accuracy and precision of data. Based on many years experience, Starna has developed sets of Certified Reference Materials, carefully selected to demonstrate compliance to national and international norms with confidence. Sets are available to suit all needs, ranging from the user with just one instrument to large laboratories, quality control departments and support and service organizations. In addition to the convenience they provide, Starna CRM sets also offer cost savings when compared to the purchase of individual references. USP Chapter <857> In May 2015 the US Pharmacopeia published a new Chapter <857> containing new instrument performance test specifications for ultraviolet and visible spectrophotometers. These become mandatory from 1st May New instrument qualification references from Starna allow laboratories to meet these new regulations. The new chapter includes requirements for Operational Qualification (OQ) and Performance Qualification (PQ): Operational Qualification (OQ) is required to demonstrate that the instrument under test is suitable for the intended use and analytical procedures for which it is employed. Performance Qualification (PQ) requires that that it can be demonstrated on an ongoing basis that the instrument continues to meet that standard. The new regulations for OQ and PQ require that laboratories can demonstrate to the regulatory authority that the spectrophotometric devices being used are qualified for wavelength and photometric accuracy, stray light and instrument resolution. New stray light and resolution tests are described that require the use of reference materials not previously specified by the USP. It is also stated in Chapter <857> that Certified Reference Materials should be obtained from a recognized and accredited source, and have value assignments with associated calculated uncertainty. Certified values must be traceable to internationally recognized primary standards. The references must be periodically recertified to maintain validity of the certification. Starna s production and calibration facilities are accredited to ISO/IEC and ISO Guide 34, meeting all international standards and regulatory requirements. Starna can supply all the Certified Reference Materials required to meet the new regulations in convenient, ready to use sets. There is also a pathway to upgrade an existing USP set if manufactured by Starna. It may be possible to provide an upgrade for third party references, but this would need to be assessed on an individual basis. Please contact Starna for more information. US Pharmacopeia Starna has developed sets of Certified Reference Materials designed to help instrument users demonstrate compliance to national and international norms with confidence. Sets are available to suit all needs, from the user with just one instrument to large laboratories, quality control departments and support and service organizations. In addition to the convenience they provide, Starna CRM sets also offer cost savings when compared to the purchase of individual references. 38

39 Instrument Qualification Sets - USP Only Certified Reference Materials recommended by the USP for instrument qualification, and traceable to NIST, are supplied in these sets. More details of the individual materials may be found elsewhere in this catalog. NEW USP 857 REFERENCES USP Instrument Qualification Set Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 & 5 mm cells Stray Light nm Sodium Iodide 10 & 5 mm cells Stray Light nm Acetone 10 & 5 mm cells Stray Light nm Sodium Nitrite 10 & 5 mm Stray Light nm Toluene in Hexane & blank cell Resolution nm Starna USP Instrument Qualification Set RM-USP $ USP Instrument Qualification Set with Linearity Check As RM-USP but with two additional Potassium Dichromate cells Potassium Dichromate 20 mg/l Photometric Accuracy nm Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 100 mg/l Photometric Accuracy nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 & 5 mm cells Stray Light nm Sodium Iodide 10 & 5 mm cells Stray Light nm Acetone 10 & 5 mm cells Stray Light nm Sodium Nitrite 10 & 5 mm Stray Light nm Toluene in Hexane & blank cell Resolution nm Starna USP Instrument Qualification Set with linearity RM-USP/3LIN $ USP Visible Transmittance/Absorbance and Wavelength Set: This set combines three neutral density filters (with blank holder) and a holmium glass filter and is a convenient way of qualifying a visible spectrophotometer for photometric accuracy and linearity, and wavelength accuracy. Neutral Density Filters (1.00, 0.50 & 0.25 A) Absorbance Accuracy nm Holmium Glass Filter Wavelength Accuracy nm Starna Neutral Density and Holmium Filter Set RM-1N3N5DHG $ NIST SRM Equivalent Sets: These SRMs are specifically cited in USP <857> as evidence of NIST traceability SRM 930e Set: 10, 20 & 30 %T Neutral Density Filters and blank holder RM-1N2N3N $ SRM 1930 Set: 1, 3 & 50 %T Neutral Density Filters and blank holder RM-N1N35N $ SRM 2930 Set: 0.1, 0.3 &92 %T Neutral Density Filters and blank holder RM-D1D39N $ Combined SRM Set: All 9 Neutral Density Filters listed above and blank holder RM-SRM9ND $ UV Absorbance Scale & Linearity validation For method validation, USP <857> recommends that absorbance linearity is checked with five or more analyte concentrations. The following Potassium Dichromate sets allow linearity to be checked over the relevant absorbsorption range from 235 nm nm. Photometric Linearity Set, 0.095A -1.5A Part Number, NIST Traceable Price Potassium Dichromate 20, 40, 60, 80, 100 mg/l & blank cell RM $ Photometric Linearity Set, 1.5A- 3.3A Potassium Dichromate 120, 140, 160, 180, 220 & 240 mg/l & blank cell RM $ Photometric Linearity Set, 0.19A - 3.3A Potassium Dichromate 40, 80, 120, 160, 200 & 240 mg/l & blank cell RM $

40 Tools for Easier Compliance Many of the references recommended by the US Pharmacopeia are also suitable for working to the standards of regulatory authorities, such as the ASTM, European Pharmacopoeia and other national bodies. European Pharmacopoeia Only Certified Reference Materials recommended by the European Pharmacopoeia for instrument qualification, and traceable to NIST, are supplied in these sets. More details of the individual materials may be found elsewhere in this catalog. EP Instrument Qualification Set Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 mm cell & blank cell Stray Light 200 nm Toluene in Hexane & blank cell Resolution nm Starna EP Instrument Qualification Set RM-EP $ EP Instrument Qualification Set with Linearity Check As RM-EP but with two additional Potassium Dichromate cells: Potassium Dichromate 20 mg/l Photometric Accuracy nm Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 100 mg/l Photometric Accuracy nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 mm cell & blank cell Stray Light 200 nm Toluene in Hexane blank cell Resolution nm Starna EP Instrument Qualification Set with Linearity RM-EP/3LIN $ EP Instrument Qualification Set with Derivative Resolution Check As RM-EP but with additional resolution reference for Derivative Spectroscopy Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 mm cell & blank cell Stray Light 200 nm Toluene in Hexane & blank cell Resolution nm Toluene in Methanol & blank cell Derivative resolution nm Starna EP Instrument Qualification Set with Derivative RM-EP/TM $

41 European Pharmacopoeia, ASTM etc EP Instrument Qualification Set with two Stray Light References Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 mm cell & blank cell Stray Light 200 nm Sodium Iodide 10mm cell Stray Light 260 nm Toluene in Hexane & blank cell Resolution nm Starna EP Instrument Qualification set with two Stray Light References RM-EP/2SL $ EP Instrument Qualification Set with three Stray Light References Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 mm cell & blank cell Stray Light 200 nm Sodium Iodide 10mm cell Stray Light 260 nm Sodium Nitrite 10 mm cell Stray Liight 391 nm Toluene in Hexane & blank cell Resolution nm Starna EP Instrument Qualification set with three Stray Light References RM-EP/3SL $ Combined Instrument Qualification Sets - USP & EP These kits will be useful for laboratories working to international regulations and for service organisations Combined USP/EP Set Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 & 5 mm cells Stray Light nm Sodium Iodide 10 & 5 mm cells Stray Light nm Acetone 10 & 5 mm cells Stray Light nm Sodium Nitrite 10 & 5 mm cells Stray Light nm Water blank 10 mm cell Stray Light nm Toluene in Hexane & blank cell Resolution nm Starna USP/EP Instrument Qualification Set RM-USP/EP $ Combined USP/EP Set with Linearity Check Potassium Dichromate 20 mg/l Photometric Accuracy nm Potassium Dichromate 60 mg/l & blank cell Photometric Accuracy nm Potassium Dichromate 100 mg/l Photometric Accuracy nm Potassium Dichromate 600 mg/l Photometric Accuracy 430 nm Holmium Oxide Solution cell Wavelength Accuracy nm Potassium Chloride 10 & 5 mm cells Stray Light nm Sodium Iodide 10 & 5 mm cells Stray Light nm Acetone 10 & 5 mm cells Stray Light nm Sodium Nitrite 10 & 5 mm cells Stray Light nm Water blank 10 mm cell Stray Light nm Toluene in Hexane & blank cell Resolution nm Starna USP/EP Instrument Qualification Set with linearity check RM-USP/EP/3LIN $

42 Product Summary and Price List Description: Part No. Price US$ Photometric Scale Verification UV Potassium Dichromate (PDC) 20 mg/l RM-02 $ mg/l RM-04 $ mg/l RM-06 $ mg/l RM-08 $ mg/l RM-10 $ mg/l RM-12 $ mg/l RM-14 $ mg/l RM-16 $ mg/l RM-18 $ mg/l RM-20 $ mg/l RM-22 $ mg/l RM-24 $ mg/l RM-60 $ PDC 2 concentrations & blank $ 1, PDC 3 concentrations & blank $ 1, PDC 4 concentrations & blank $ 1, PDC 7 concentrations & blank $ 2, PDC 5 Linearity set RM $ 2, PDC 6 Linearity set RM $ 2, PDC 6 Linearity set RM $ 2, PDC 6 Linearity set RM $ 2, Far UV Photometric Scale Verification Nicotinic Acid 6mg/l RM-1A $ mg/l RM-2A $ mg/l RM-3A $ mg/l RM-4A $ concentration set RM-1A2A3A4A $ 2, Deep UV Photometric Scale Verification Starna TS8 TS8 & Blank RM-DUV/195 $ 3, Photometric Scale Verification UV/VIS Metal on Quartz 1% & Blank RM-Q1 $ 1, % & Blank RM-Q3 $ 1, % & Blank RM-1Q $ 1, % & Blank RM-3Q $ 1, % & Blank RM-5Q $ 1, % & Blank RM-9Q $ 1, %, 50% & Blank RM-Q35Q $ 1, %, 30%, 90% & Blank RM-1Q3Q9Q $ 2, Starna Green 25X concentration & Blank RM-1SG $ X concentration & Blank RM-2SG $ X concentration & Blank RM-3SG $ X concentration & Blank RM-4SG $ concentrations & Blank RM-1SG2SG3SG4SG $ 1, Screw Cap Vials 1 Vial 10X concentration RM-SG10-SC1 $ Vials 10X concentration RM-SG10-SC2 $ Vials 10X concentration RM-SG10-SC4 $ Description: Part No. Price US$ UV/VIS Wavelength Accuracy Holmium Oxide RM-HL $ Didymium Oxide RM-DL $ Samarium Oxide RM-SL $ Far UV Wavelength Accuracy Rare Earth RM-RE $ Stray Light USP Potassium Chloride RM-KC/5 $ Potassium Iodide RM-KI/5 $ Sodium Iodide RM-SI/5 $ Acetone RM-AC/5 $ Sodium Nitrite RM-SN/5 $ Stray Light set $ 1, Stray Light set $ 2, Stray Light set RM-ACKCSISN/5 $ 3, Stray Light EP Sodium Iodide RM-SI $ Potassium Iodide RM-KI $ Lithium Carbonate RM-LC $ Potassium Chloride RM-KC $ Sodium Chloride RM-SC $ Sodium Nitrite RM-SN $ Acetone RM-AC $ SBW Bandwidth Calibration Toluene in Hexane RM-TX $ UV Bandwidth Derivative Spectroscopy Toluene in Methanol RM-TM $ Instrument Resolution Benzene Vapor RM-BZ $ UV/VIS Wavelength Accuracy Filters (Glass) Holmium Glass filter RM-HG $ Didymium Glass filter RM-DG $ Absorbance scale & Linearity Visible Neutral Density Filter sets & NIST equivalents 10, 20, 30%T (SRM930e) RM-1N2N3N $ 1, , 3, 50%T (SRM1930) RM-N1N35N $ 1, , 0.3, 92%T (SRM2930) RM-D1D39N $ 1, Combined SRM Neutral Density Set. RM-SRM9ND $ 3, , 30, 52%T, HG RM-1N3N5DHG $ 1, Single Filter RM-xx $ Four ND Filter set RM-4ND $ 1, Six ND Filter set RM-6ND $ 2, Nine ND Filter set RM-9ND $ 3, Microplate Absorbance scale and Wavelength Accuracy Visible Microplate Reference RMMR-1N3NN1N3HG $ 2, Microplate Cell Adaptor SCP-96-8R $ mm Quartz Cell with PTFE Screw Cap 1.30-Q-10-ST $

43 Description: Part No. Price US$ Validation of 260/280 ratio DNACON Sealed Cell RM-DNA $ Screw Cap Vials 1 Vial 5X concentration DNACON5X-SC1 $ Vials 5X concentration DNACON5X-SC2 $ Vials 5X concentration DNACON5X-SC4 $ Vial 10X concentration, DNACON10X-SC1 $ Vial 10X concentration, DNACON10X-SC2 $ Vial 10X concentration, DNACON10X-SC4 $ Absorbance scale & Linearity NIR Neutral Density Filter sets 20%T RM-R1 $ %T RM-R3 $ %T RM-I1 $ , 25 & 33%T RM-R1R3I1 $ 1, Photometric Scale Verification NIR Metal on Quartz 1% & Blank RM-NQ1 $ 1, % & Blank RM-NQ3 $ 1, % & Blank RM-1NQ $ 1, % & Blank RM-3NQ $ 1, % & Blank RM-5NQ $ 1, % & Blank RM-9NQ $ 1, %, 50% & Blank RM-NQ35NQ $ 2, %, 30%, 90% & Blank RM-1NQ3NQ9NQ $ 2, %, 3%, 50% & Blank RM-NQ1NQ35NQ $ 2, Wavelength Accuracy NIR NIR transmittance RM-NIR $ 1, NIR transmittance/reflectance RM-NIR/T $ 1, Stray Light NIR Chloroform RM-CHCL3 $ 1, Mid Infrared Wavelength Reference Polystyrene 38 um RM-1921/38 $ Mid & Near Infrared Wavelength Reference Polystyrene 65 um RM-1921/65 $ Description: Part No. Price US$ Fluorescent Sensitivity Reference Ultra Pure Water RM-H20 $ Fluorescent Wavelength Reference Quinine Sulfate 1 x 1.0 mg/l, RM-QS $ 1, x 1.0 mg/l & blank RM-QS00 $ 1, concentrations, & blank RM-2QS00 $ 2, concentrations, & blank RM-3QS00 $ 2, concentrations, & blank RM-4QS00 $ 3, Raman Wavelength Reference Cyclohexane RM-C6H12 $ 1, Instrument Qualification Sets USP Basic USP set RM-USP $ 4, Basic USP set,linearity RM-USP/3LIN $ 5, Visible T/A & Wavelength RM-1N3N5DHG $ 1, , 20, 30%T (SRM930e) RM-1N2N3N $ 1, , 3, 50%T (SRM1930) RM-N1N35N $ 1, , 0.3, 92%T (SRM2930) RM-D1D39N $ 1, Combined SRM Neutral Density Set RM-SRM9ND $ 3, USP Absorbance scale and linearity validation Linearity set 0.095A - 1.5A RM $ 2, Linearity set 1.5A - 3.3A RM $ 2, Linearity set 0.19A - 3.3A RM $ 2, Instrument Qualification Sets EP & other Pharmacopoeia Basic EP set RM-EP $ 3, Basic EP set with Linearity RM-EP/3LIN $ 3, Basic EP set with Derivative RM-EP/TM $ 4, Basic EP set 2 Stray Lights RM-EP/2SL $ 3, Basic EP set 3 Stray Lights RM-EP/3SL $ 4, Combined Instrument Qualification Sets USP, EP & other Pharmacopoeia Basic combined set RM-USP/EP $ 6, Basic combined set with Linearity RM-USP/EP/3LIN $ 7,