GI-150 SAMPLE BOTTLE GUIDE
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1 GI-150 SAMPLE BOTTLE GUIDE CHEMICAL ANALYSIS INORGANIC BIOLOGICAL GENERAL RADIOACTIVITY HEAVY METALS MERCURY NUTRIENTS - TOTAL NUTRIENTS - FILTERABLE 600mL Plastic 100mL Glass 120mL Plastic (GL100) (PT120) ALGAL & ODOURS CHLOROPHYLL 1 L Black Plastic (BLKPT1) A B PT600 None None No, Ice PT1250 None None No, Ice PT355 Acid Washed None, Ice GL100 Acid Washed None, Ice PT355 None None No, Ice A. PT120 None None - Filtered Air gap, Ice B. PT120 None None - Air gap, Ice PT355 None None Air BLKPT1 None None Air General Cations (7 days) **ph (6 hours) *Conductivity (28 days) *Colour (48hrs),*Turbidity (24hrs) *Alkalinity (24 hours) **Gross Alpha & Beta (28 days) *All Metals (excluding Mercury) (28 days) Includes cations Calcium, Magnesium, Sodium and Potassium *Mercury (28 days) *Chloride (28 days) *Fluoride (28 days) *OXN (28 days) *Ammonia (28 days) *TKN (28 days) *Total P (28 days) ***Blue Green Algae, see preservation below ***Odour Test *Chlorophyll (24-48 Hours) Container is pre acid washed with Green Lid Container is pre acid washed with Blue Lid. Place containers in double zip locked bags to avoid lids being contaminated. Filtered Sample will require filter equipment Algae holding time increased to 28 days when preserved with Lugol s solution Freshwater samples 1:100 by volume Marine samples 1:200 by volume Version 1.0.D 20/02/2015 Page 1 of 5
2 WASTEWATER GENERAL AND BOD CYANIDES SULPHIDES GREASE & OILS PHENOLS SLUDGES, SOLIDS, & SOILS 600mL Plastic 1L Glass (GL1000) 1L Amber Glass (AG1000) 500mL Plastic Pot (PP500) SLUDGE & SEDIMENTS (Microbiological analyses) Plastic Pot GIARDIA & CRYPTOSPORIDIUM (wastewater only) 2 x PT1250 None None No, Ice PT600 None NaOH No, Ice PT355 None Zinc Acetate, Ice GL1000 None None, Ice AG1000 None None No, Ice PP500 None None None PT600 Sterile Sodium Thio, Ice PT1250 Sterile Sodium Thio, Ice *Biological Oxygen Demand *Suspended Solids (24 hrs) *Chemical Oxygen Demand (28 days) *Cyanide (24 hours) *Sulphides, 28 days for preserved samples 24 hours for non preserved samples *Grease (28 days) *MBAS (48hrs) ** (24 Hours) #E.coli (24 hours), Filamentous bacteria Amoebae Naegleria fowleri (48hrs as per in-house valid.) Cryptosporidium and Giardia (48 hours as per USEPA 1623) NaOH pellet dosed Samples to be taken in predosed container. Do not rinse. Fill initially with small air gap, invert to mix pellets, squeeze out remaining air. Zinc Acetate Dosed Samples to be taken with the minimum amount of aeration and the bottle completely filled Caution to not overfill container Required to place pot in double zip locked bags Aseptic preparation is mandatory Place bottles in double zip locked bags for storage on ice Aseptic Preparation is mandatory 2x1.25L Pet Bottles be used Version 1.0.D 20/02/2015 Page 2 of 5
3 CHEMICAL ANALYSIS ORGANIC PHENOL VCH NDMA MX GENERAL ALGAL TOXINS MIB/GEOSMIN, TCA 355ml Plastic 10L Plastic 1L Glass (GL1000) (APT1250 Alfoil wrapped) (JC1) DOC, TOC, MIB, GEOSMIN, TCA, HAAFP, THMFP, GLYPHOSATE DISINFECTION by PRODUCTS 355ml Plastic 600ml Plastic PT1250 None - Ammonium Chloride No, Ice ***Halogenated Phenols PT355 None Ascorbic Acid, Ice ***VCH APT1000 None Sodium Thio, Ice ***NDMA JC1 None Ascorbic Acid, Ice ***MX GL1000 None None No Air ***Organochlorides ***Organophosphates ***Acid herbicides ***GCMSSCANs ***Diesel, VOC, BTEX, MTBE, Fipronyl, Haloxyfop ***Atrazine metabolites, Simazine ***Formaldehyde TPH/TRH PT1250 None None, Ice ***MIB, GEOSMIN ***TCA are performed by CLSA for lower detection limits. ***Algal Toxins PT355 None None No Air ***Dissolved Organic Carbon, Total Organic Carbon ***Total Carbon ***MIB, GEOSMIN, TCA ***Glyphosate ***Formation Potential of THM and HAA PT355/PT600 None Ammonium Chloride No Air ***Haloacetic Acids ***Chloracetic Acids ***DBP_551 ***THM 100mg/L Ammonium Chloride dosed 250mg/L Ascorbic Acid dosed 150mg/L Sodium Sulphite for Chloramine < 4.0mg/L Wrap entire bottle in foil. Amber glass bottles or black plastic bottles can be used. 5g Ascorbic Acid dosed Amber glass bottle can also be used. 100mg/L Ammonium Chloride dosed Single analysis 355mL bottle is sufficient for > 2 analyses 600mL bottle required Version 1.0.D 20/02/2015 Page 3 of 5
4 MICROBIOLOGICAL GENERAL LEGIONELLA SULPHITE & SULPHATE REDUCING BACTERIA 300 / 600mL Sterile Plastic 300mL Sterile Plastic 300mL Sterile Plastic (PT300 / 600) (PT300) (PT300) CAMPYLOBACTER & SALMONELLA 2 x 600mL Sterile Plastic # Holding times as per AS/NZS2031 ICE AMOEBAE CRYPTOSPORIDIUM & GIARDIA Plastic Pot 600mL Sterile Plastic 2 x10l Plastic (JC1) PT300 / 600 Sterile Sodium Thio, Ice PT300 Sterile Sodium Thio, Ice PT300 Sterile Sodium Thio, Ice, Ice, Ice, No Ice JC1 Sterile Sodium Thio Air #E.coli (24 hours) #Faecal Coliforms (24 hours) #Enterococcus (24 hours) #Iron Bacteria (24 hours) #Pseudomonas (24 hours) #Plate Counts (24 hours) #Bacteriophages and f RNA phage ( 24 hours) #Legionella (24 hours) Samples from Warm or Hot Water Systems require NO FLUSHING or flame sterilisation of sample tap prior to sampling. #Sulphite reducing Clostridia including Clostridium perfringens (24 hours) #Sulphate Reducing Bacteria (24 hours) #Campylobacter (C.jejuni, C.coli) (24hours) #Salmonella spp.(24 hours) #E.coli (24 hours), ***Amoebae Naegleria fowleri (48 hours as per in-house validation) AMOEBAE samples are not to be chilled Cryptosporidium and Giardia (48 hours as per USEPA 1623) 2x 600mL bottles to be used. Aseptic preparation is mandatory Place bottles in double zip locked Not Iced or chilled bags. Aseptic Preparation is mandatory 2x10L Plastic Containers (jerry cans) to be used. Version 1.0.D 20/02/2015 Page 4 of 5
5 Version 1.0.D 20/02/2015 Page 5 of 5
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