Passive Systems: History, function, promise, and optimization Christopher J Gobler, Ph.D
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1 Passive Systems: History, function, promise, and optimization Christopher J Gobler, Ph.D SoMAS, Stony Brook University NYS Center for Clean Water Technology 1
2 What is a passive system? FL DoH: a type of onsite wastewater treatment system that utilizes no mechanical components other than one effluent pump and uses a reactive media for denitrification. Consistent, reliable, low-energy, and low-maintenance. CCWT is investigating three types of passive systems: Wetlands, permeable reactive barriers, and nitrogen removing Biofilters (NBRs). 2
3 Wetlands Designed to improve the quality of effluent Unplanted Biofilters, sand filters etc. Planted Surface Flow Subsurface Flow PhD student, Samantha Roberts Vertical Flow Horizontal Flow Constructed Wetlands 3
4 Constructed Wetlands Subsurface Flow: Horizontal Gravel and sand-filled channel Wastewater flows horizontally across system Modest treatment, both microbial and vegetative From Tilley et al (2008) 4
5 Subsurface Flow: Vertical Constructed Wetland Wastewater drains vertically through the filter layers towards a drainage system at the bottom. Enhanced microbial and vegetative treatment. Optimized via recirculation and additions of denitrifying tanks, layers (e.g. wood chips). From Nivala et al (2013) 5
6 Permeable reactive barriers (PRB) to remove nitrogen and other contaminants PRBs contain a carbon source (wood, vegetable oil) in a permeable media to bring in groundwater and promote nitrogen removal via denitrification containing high nitrate before it enters surface waters. Most effective at the headwaters of streams and/or coves where groundwater discharge is concentrated. CCWT is collaboratively studying PRB s in Southampton and East Hampton Towns; CCWT measurements will unlock the black box, expanding understanding. 6
7 Passive systems to replace current onsite wastewater systems 7
8 Standard drain field system Most common on-site wastewater disposal approach in US. Very rarely used on Long Island. More nitrogen removal than Long Island leach-pit systems due to proximity to surface. Shallow depth (< 2 ft) wellsuited for coastal regions experiencing sea-level rise. 8
9 Depth to groundwater on Long Island SoMAS estimates 7 ft of sea level rise this century. 9
10 Standard drain fields still leach nitrogen Septic Tank NO 3 - Standard leaching trench NO 3 - NO 3 - NO 3 - NO 3 - NO 3 - NO 3 - NO 3 - NO 3 - Groundwater Flow NO 3 - NO 3 - NO 3 - NO 3-10
11 11
12 Nitrogen Removing Biofilters (NRB) 12
13 Nitrogen Removing Biofilters (NRB) Lignocellulose = wood, chips, dust, etc Carbon source to promote denitrification 13
14 Nitrogen Removing Biofilters (NRB) High O 2 No O 2 Viking ship analogy! 14
15 Nitrogen Removing Biofilters in Seminole County, FL by Hazen and Sawyer, Damann Anderson, P.E. Pipe boot Gravel Underdrain 15
16 Full-scale, vertically stacked biofilter Stage 1 and 2a (sand above sand/ligno mix) Stage 2 Elemental Sulfur in tank Drip irrigation Final effluent dispersal Surface Area 728 ft ft ft 2 Media 18 Fine Sand above 9 lignocellulosic and fine sand mix, at 50/50 ratio 12 elemental sulfur & oyster shell mix, 90/10 ratio N/A 16
17 Full-scale system results over 500+ days operation 17
18 Investigating Non-proprietary Means of Nitrogen Removal Massachusetts Alternative Septic System Test Center Director, George Heufelder 18
19 Nitrogen Removing Biofilters at MASSTC Column, small-, and full-scale systems tested. Systems have examined differing depths of layers, differing amounts of lignocellulose (wood chips), and saturated v. unsaturated. MASSTC has been monitoring systems for several years; CCWT has data since January. 19
20 Small-scale (10 x 10), unsaturated system hydraulically loaded at code-prescribed rate Total Nitrogen (mg/l) Mean 5.6 mg/l TN Median 3.3 mg/l TN Date LC TN LC Infl TN 20
21 Large-scale saturated system, 26 x 26 ft 21
22 Large-scale saturated system Hydraulic Loading 0.6 gal/day/ sq. ft, (220 gallons/day), Alternately dosed distribution laterals 22
23 Large-scale saturated system Total nitrogen (mg/l) Total Nitrogen Mean 8.0 mg/l TN Median 4.5 mg/l TN 0 BLC TN BLC Septic Tank TN BLC Inf TN 23
24 Full scale, unsaturated system loaded at code-prescribed rate 24
25 CCWT Design Charrette, March 2016 Two-day gathering of regional and national experts on NRBs. Consensus on testing Long Island native materials. Consensus building on function and optimal, next generation design. 25
26 Lined, saturated NRB GeoMat system for low pressure STE dosing Underdrain, within denite media 26
27 Unlined, unsaturated NRB GeoMat system for low pressure STE dosing 27
28 Replaceable woodchip biofilter GeoMat or other system for low pressure STE dosing Nitrified effluent to wood chip denitrification biofilter, in tank Denitrification 28
29 Sourcing native sands and wood products 29
30 Survey of sand from Suffolk mines Sample % Organic porosity ALK mg CaCO3/L MASSTC Silt MASSTC Loam East Coast Coarse.Fine East Coast Bank East Coast Concrete East Coast Fine.Fine Ranko Fls Ranko Scs Ranko Pitt Roanoke Fine Sand Roanoke State Sand Roanoke Fine.Recovery
31 We have a winner 31
32 Long Island native sands and wood chips have arrived in Massachusetts 32
33 System installation in the coming weeks! 33
34 Suffolk County Reclaim Our Waters, demonstration of innovative/alternative onsite wastewater treatment systems, phase II CCWT applied to the County s phase II, Request for Expressions of Interest ( RFEI ) using the NRB. Summer/Fall Phase II Design and Installations of NRB Begin Fall 2016, Monitoring of NRB Provisional approval of NRB? 34
35 Performance of Saturated and Unsaturated Nitrogen Removing Biofilters (NRB) at MASTCC Xinwei Mao, Stuart Waugh, Molly Graffam, Samantha Roberts, Kylie Langlois, Patricia Clyde, Jeanette Lee, Megan Ladds, Benjamin Karmar NYS Center for Clean Water Technology 35
36 Sampling systems description The graph is from G. Heufelder, Barnstable County 36
37 Sampling systems description----schematic top layer top layer top layer sand layer 6 18 sand layer 18 sand layer 18 sand layer + 50% sawdust 18 silt layer + 20% sawdust 18 sand layer + 50% sawdust Sand system (Saturated) X Silt system (un-saturated) Y Sand system (un-saturated) Z 37
38 Sampling systems description----field sites System X System Y Effluent port Pan Lysimeter Sample frequency: Jan, 2016 and Apr,
39 Sampling systems description-cont d Nitrified percolate On-ground denitrifying bioreactor Column system (Saturated) C 39
40 Field sample analyses BOD 5 TSS Alkalinity Nutrients Microbiology PPCPs 40
41 BOD 5 (mg/l) BOD 5 (biochemical oxygen demand) X-saturated Jan Apr Y-unsaturated Jan Apr Z-unsaturated Jan Apr ~85-97% removal ~97-99% removal ~87-97% removal 7-day effluent BOD requirement for surface water discharge, New York State CFR Part
42 TSS (Total Suspended Solids) X-saturated Jan Apr Y-saturated Jan Apr Z-saturated Jan Apr TSS (mg/l) influent effluent 0 influent effluent 0 influent effluent ~65-80% removal ~84-95% removal ~91-92% removal 7-day average effluent TSS requirement for surface water discharge, New York State 42
43 Alkalinity---- important for nitrification X-saturated Jan Apr Y-unsaturated Jan Apr Z-unsaturated Jan Apr Alkalinity (mg CaCO 3 /L) Recommended residual alkalinity to maintain system s ph 43
44 Nutrients (Total Nitrogen removal) TN (mg/l) X-saturated Jan Apr 10 mg/l Y-unsaturated Jan Apr Z-unsaturated Apr ~71-90% removal ~79-90% removal ~73% removal *the influent and effluent data are the average of three biological replicate. 44
45 Nutrients (nitrogen balance in the system) Nitrogen species (mg-n/l) X (saturated denitrification layer) Influent DON NH4+ NO3-60% TN removal Nitrification Layer 90% TN removal Effluent captured in liner Nitrogen species (mg-n/l) Y (unsaturated denitrification layer) Influent DON NH4+ NO3-31% TN removal Nitrification Layer Denitrification may occur at both nitrification and denitrification zones. 79% TN removal Effluent captured in liner * Data is from trip in Jan,
46 PO4-P (mg/l) Nutrients (phosphorus removal) X-saturated Y-unsaturated Jan Apr mg/l Jan Apr Z-unsaturated Jan Apr ~99-100% removal ~80-92% removal ~96-97% removal 30-day average effluent TP requirement for surface water discharge, New York State 46
47 Microbial analysis----e.coli (indicator of pathogens) 1.E+06 1.E+05 X-saturated Jan Apr 1.E+06 1.E+05 Y-unsaturated Jan Apr 1.E+06 1.E+05 Z-unsaturated Jan Apr 1.E+04 1.E+04 1.E+04 E.coli (cfu) 1.E+03 1.E+02 1.E+03 1.E+02 1.E+03 1.E+02 1.E+01 1.E+01 1.E+01 1.E+00 1.E+00 1.E % removal 100% removal 100% removal 47
48 Microbial analysis----nitrogen cycling pathways example: PCR of hzoa gene amoa Anammox hzo-a Positive control nir-s, nir-k negative control PCR (polymerase chain reaction) for all collected samples (liquid/core) 48
49 Microbial analysis----presence of functional genes Function Biomarker (gene) inf Saturate system X Un-saturate system Y Un-saturate system Z X-1 X-2 X-3 Y-1 Y-2 Y-3 Z-1 Z-2 Z-3 C nitrification amoa (arc) amoa (bac) denitrification Nir-S Nir-K anammox hzo-a no amplification Presence (strong band) Presence (weak band) N.A. 49
50 Microbial analysis----in progress I-tag 16S sequencing Metagenomics Metatranscriptomics what microorganisms are present in different parts of existing systems PCR presence/absence of each functional guild in different parts of existing systems quantitative- PCR abundance and activity of each functional guild in different parts of existing system. 50
51 PPCPs (Pharmaceuticals and Personal Care Products) DEET Carbamazepine TCEP Sulfamethoxazole Pesticide Persistent in the environment The most frequently detected in US GW Anticonvulsant Carcinogenic to rats Most frequently detected PPCP in two studies of LI GW Widely used flame retardant found at higher levels than other PPCPs in drinking water Commonly prescribed antiobiotic Danger of leading to antibioticresistant bacteria They have all been measured in groundwater near OSWTs. They are poorly treated by full-scale activated sludge treatment systems. 51
52 PPCPs (Pharmaceuticals and Personal Care Products) DEET Carbamazepine TCEP Sulfamethoxazole Influent 19% * 5-6% * 23% * 54-58% # Removal Efficiency 90% 99% 99.9% Influent C X Y Z Influent C X Y Z Influent % % % % C X Y Z Influent C X Y Z Over 95% removal of all 18 PPCPs detected in influent Removal efficiency in full scale wastewater treatment plants * Reemtsma, 2008 # EPA, 2010; Lara-Martin,
53 Summary These results demonstrate MASSTC s passive NRBs are highly efficient at removing nitrogen, even in the winter. The results also indicated nitrogen loss at both nitrification and denitrification zones in NRBs. The NRBs investigated in this study can efficiently remove BOD, pathogen indicators, and all PPCPs detected. Molecular biology tools (e.g. sequencing, qpcr) are extremely useful to study the microbial ecology and could be used to evaluate, predict and improve the performance of existing and novel on-site wastewater treatment systems. 53
54 Supporting Materials 54
55 0 0% DEET 90% -1 99% % % Sulfamethoxazole -1 90% 99% % -3 C X Y Z 55
56 TCEP 1 0% 0 90% -1 99% % -3 C X Y Z Influent C X Y Z 56
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