Cell Culture Products LABORATORY GLASSWARE

Transcription

1 Cell Culture Products LABORATORY GLASSWARE

2 2 ACE GLASS Incorporated, a leader in the design and manufacture of glass labware and scientific equipment, has been supplying research laboratories with quality products for over seventy years. Since 1936, ACE products have been a part of labs performing analytical functions, as well as life sciences labs. In this new Cell Culture Products Catalog, ACE GLASS presents a line of products for cell culture labs and offer glass lab products designed specifically for use by the cell culturist. ACE laboratory glass products are made from borosilicate glass and meet USP and ASTM Type 1, Class A specifications, so you can be assured of quality and performance. ACE GLASS has always been in the forefront of scientific innovation and new product development. We welcome your suggestions for enhanced modifications or new product ideas. BOTTLE Solution, Plastic Coated Solution bottles with serrated vacuum take-off. Bottles are coated to neck with plastic safety coating. This translucent coating forms a protective film around the glass that will help contain both flying particles and dangerous chemicals should the glass break. Maximum temperature limit 80 C. Higher temperature coatings available on special order. All sizes have the sloping shoulders of the carboy style of bottle. Necks are tooled for uniform fit with stoppers. Use with 7 / 16 inch or ½ inch I.D. Tubing. Cap., Gal. Dia., Height, Rubber Stopper No. Qty. Liters CLONING CYLINDER Clone a single cell or group of cells by surrounding them with this glass cylinder. Dip the end of the cylinder into a sterile silicone grease before pressing to the bottom of a culture flask to create an isolated well. O.D.., Height, Qty /Cs /Cs /Cs CLONING CYLINDER Cylinders for use in the Disinfectant Procedure as described in the 1992 J.A.O.A.C. Hard Surface Carrier Test for Efficacy Testing of Disinfectants: Collaborative Study. Tubes are glazed on both ends. O.D., Height, Qty /Cs /Cs FILLING BELL Aseptic For aseptic filling of vessels. Rubber tubing is attached to the top hose connection, the receiving vessel is placed inside the bell. I.D., Height, Qty. For Use With Hose O.D., Tubes Tubes Bottles Bottles Bottles

3 3 FLASK Culture, Fernbach, Triple Baffled, Beaded Neck Wide mouth flask designed for culturing organisms requiring a large surface-area-to-volume ratio. Triple baffled on the bottom edges to achieve maximal oxygen transfer to culture medium. Beaded neck is 63 I.D. and accepts cotton plugs or rubber stoppers. Caution! Do not place on direct heat source. Dimensions Qty x x FLASK Culture, Fernbach, Baffled, Plain Neck, without Closure With a long-style 38 neck for plastic or Stainless Steel closures. Triple baffles are located at the center of the bottom to achieve maximal oxygen transfer to culture medium. Caution! Do not place on direct heat source. Closure Size, Dimensions Qty x x FLASK Culture, Fernbach, Baffled, Screw Cap Wide mouth flask designed for culturing organisms requiring a large surface-area-to-volume ratio. Triple baffled on the bottom outside edges to achieve maximal oxygen transfer to culture medium. Supplied with GL45 polypropylene cap. Caution! Do not place on direct heat source. Dimensions Qty x x FLASK Culture, Fernbach, 38 Neck, without Closure Designed for culturing organisms requiring a large surface-to-volume ratio. With long 38 neck that takes Stainless Steel or plastic closures. Without baffles. Closure Size, Dimensions Qty x x

4 4 FLASK Shaker, with Three Side Baffles, without Closures Long-style neck with three side baffles to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces splashing and is designed for polypropylene closures. Neck O.D., Qty x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs FLASK Shaker, without Closure With long-style neck for use with plastic closures. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Closure Size, Qty x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs FLASK Shaker, with Three Deep Baffles, without Closures Long-style neck with three extra deep baffles to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces splashing and is designed for polypropylene closures. Neck O.D., Qty x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs FLASK Shaker, Baffled, with Beaded Neck Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Qty x /Cs x /Cs x /Cs x 215 6/Cs x 285 3/Cs

5 5 FLASK Shaker, Deep Baffles, with Beaded Neck Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Qty x /Cs x /Cs x /Cs x 215 6/Cs x 285 3/Cs FLASK Shaker, with Three Side and Three Bottom Baffles, without Closures Similar to 3887, except these long-style neck flasks have three extra-deep baffles on side and bottom to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces splashing and is designed for polypropylene closures. Neck O.D., Qty x /Cs x /Cs x /Cs x /Cs x /Cs x /Cs FLASK Shaker, with Four Bottom Baffles, without Closures With four bottom baffles. Long-style 38 neck. Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. With constricted neck to reduce spillage and foaming. Qty x /Cs x /Cs x /Cs FLASK Trypsinizing, with Beaded Neck, Baffled Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Heavy beaded neck accepts cotton plugs. Four extra-deep baffles enhance vigorous agitation. Qty x 100 3/Cs x 115 3/Cs x 130 3/Cs x 175 3/Cs x 215 2/Cs x 285 2/Cs x 350 2/Cs

6 6 FLASK Trypsinizing, with Sidearm, Screw Caps, Baffled Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Phenolic screw cap with rubber liner on neck and sidearm. Designed with well below the sidearm to prevent unseparated tissues from being poured out of the flask. Three extra-long, extra-deep baffles promote vigorous agitation. Height of Baffles, Center Neck G.P.I. Sidearm G.P.I. Qty x /Cs x /Cs x /Cs FLASK Media Storage and Dispensing, Bottom Hose Used for sterile dispensing; use with an aseptic filling bell. Bottom ½ inch hose connection assures aseptic drainage. With beaded rim. Stopper Size Qty x 130 #6 6/Cs x 183 #6 6/Cs x 225 #9 6/Cs x 290 #9 3/Cs x 355 #10 3/Cs x 395 #11 2/Cs FLASK Nephelo Culture, without Closures Long-style neck accoodates a 25 Stainless Steel or plastic closure. Ideal for growing, and mixing of aerobic and anaerobic cultures. Side arm fits standard spectrophotometers. Sidearm Size, Qty x 130 4/Cs x 130 4/Cs FLASK Nephelo Culture, with Screw Cap Threaded neck with cleanout port and angled sidearm. Ideal for growing and mixing of aerobic and anaerobic cultures. Supplied with GPI # and GPI # nontoxic, rubber-lined phenolic screw caps. Sidearm Size, Qty x 130 4/Cs x 130 4/Cs x 130 4/Cs x 130 4/Cs x 130 4/Cs x 130 4/Cs x 130 4/Cs x 130 4/Cs x 130 4/Cs

7 7 FLASK Nephelo Culture, Triple Baffled, without Closures Long-style neck accoodates a 25 Stainless Steel or plastic closure. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Has standard cuvette size side arm. Sidearm Size, x x x FLASK Nephelo Culture, Triple Baffled, with Screw Cap Threaded neck with cleanout port and depressed sidearm. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Supplied with GPI # and GPI # nontoxic, rubber-lined phenolic screw caps. Case/4. Sidearm Size, x x x x FLASK Filtering, Heavy Wall, with Ace-Safe Thread Hose Connection With tooled neck for uniform stopper fit. Side hose connection is #11 Ace-Thred that accepts a PTFE Tubing Connector with silicone O Ring and Nylon bushing to make an easier, safer, more convenient vacuum hose connection. Simply slide bushing over connector, attach hose to serrated end, insert into Ace-Thred and tighten until O Ring compresses, making a leaktight seal. Two connectors are available: one, Code -09, for ¼ inch I.D. tubing with a bore of.125 inches (3.18); and one, Code -10, for 3 / 8 inch I.D. tubing with a bore of.187 inches (4.75). Complete item consists of Flask, Tubing Connector (-09 or -10) and Bushing. Note! One connector for all capacity flasks. Rubber Stopper For ¼ inch Tubing For 3 / 8 inch Tubing Cap., Number Qty. Qty

8 8 150 TUBE Gas Dispersion Outside diameter of tube 8, total length of tube 150. All porosities of a given size are priced the same. Disc Dia., (B) Por. A Por. B Por. C Disc Dia., (B) Por. D Por. E B B TUBE Gas Dispersion Outside diameter of tube 8, total length of tube 150. All porosities of a given size are priced the same. Disc Dia., (B) Por. A Por. B Disc Dia., (B) Por. C Por. D TUBE Gas Dispersion Outside diameter of tube 8, total length of tube 150. Bottom is 2 thick. Disc Dia., (B) Por. A Por. B Por. C B ACE Designation Porosity Maximum Pore Dia. Range (micron) Corning Equivalent Kimble Equivalent Most Frequent Uses A EC ( ) EC ( ) Coarse filtration. Gas Dispersion B Coarse filtration. Gas Dispersion C C (40-60 C (40-60) Filtration. Gas Dispersion D M (10-15) M (10-15) Filtration and extraction E 4-8 F (4-5.5) F (4-5.5) Filtration and extraction VF VF (2-2.5) General bacteria filtrations UF UF ( ) General bacteria filtrations

9 9 TUBE Gas Dispersion For fine dispersion. Porous tip approximately 5 or 7 O.D. x 10 length. 7 O.D. tube fits #7 Ace-Thred. Stem O.D. x Overall Length, Por. A Por. B Por. C 5 x x x x Stem O.D. x Overall Length, Por. D Por. E 5 x x x x TUBE Gas Dispersion With porous fritted glass 10 x 30 tip. Available in 150, 300, 400, and 500 overall lengths. 10 O.D. on all sizes, fits through #11 Ace-Thred. Length, Por. A Por. B Por. C Length, Por. D Por. E FUNNEL Filter, Hirsch Useful where it is necessary to wash the precipitation and redissolve with chemicals which would attack filter paper. Angle of funnel 60. Cap., Disc Dia., Height Above Disc, Dia. at Top, Por. C Por. D

10 10 A New Concept in Lab Filtration Apparatus! No clamp, no leakage! Using Ace-Thred design, filter funnel and filter flask thread together without the silicone stopper and troublesome clamping device. Nylon membrane retaining ring completes seal. Nylon adapters standard, but you can special order PTFE or other polymers. Better membrane utilization! Accoodates the 47 and 25 membranes and uses 45 of the 47 grid vs. only in older designs. Removable frits! Unit disassembles completely for easy cleaning. Uses removable frits of different porosities, from extra coarse to extra fine, instead of expensive, sealed-in frits. FILTRATION APPARATUS 25 Ace Glass introduces a new concept for lab filtration apparatus. Using our famous Ace-Thred design, we are able to thread the filter funnel and filter flask together without the silicone stopper and troublesome clamping device. This design also eliminates leakage problems. The filter unit has four basic parts; the funnel, flask and nylon adapter piece and a removable glass fritted disk. The unit can be completely disassembled for easy cleaning. Instead of a sealed-in glass fritted support, the disks can be easily removed and cleaned and offer the availability of different frit sizes, from extra coarse to extra fine. The adapters are also available in PTFE or other polymers on special order. The assembly is autoclavable. Complete Filtration Apparatus includes: 500 Filter Flask w/ace-thred top, & #7 Ace-Thred & Ace-Safe connector, Nylon Adapter w/#25 Ace-Threds, 25 Porosity B ( micron) fritted disc, nylon ring, 100 graduated funnel with Ace-Thred bottom. Description 500 filter flask with #25 Ace-Thred top and #7 Ace-Safe connector filter flask with 24/40 outer joint top and glass hose barb side port Nylon adapter with #25 Ace-Thred both ends Nylon adapter with #25 Ace-Thred one end and 24/40 inner joint bottom funnel with #25 Ace-Thred bottom, graduated Porosity B fritted disk ( micron) Porosity A fritted disk ( micron) Porosity C fritted disk (25-50 micron) Nylon ring Complete FILTRATION APPARATUS 47 A 47 version of 3700 Filtration Apparatus, listed above. Complete Filtration Apparatus includes: 1000 Filter Flask w/ace-thred top, & #7 Ace-Thred & Ace-Safe side port connector, Nylon Adapter w/#25 Ace-Thred bottom, & #50 Ace-Thred top, nylon ring 25 Porosity B ( micron) fritted disc, 500 graduated funnel w/#50 Ace-Thred bottom. The assembly is autoclavable. Description 1000 filter flask with #25 Ace-Thred top and #7 Ace-Safe connector filter flask with 24/40 outer joint top and glass hose barb side port Nylon adapter with #25 Ace-Thred bottom and #50 Ace-Thred top Nylon adapter with #50 Ace-Thred top and 24/40 inner joint bottom funnel with #50 Ace-Thred bottom, graduated Porosity B fritted disk ( micron) Porosity A fritted disk ( micron) Porosity C fritted disk (25-50 micron) Nylon ring Complete

11 11 TISSUE HOMOGENIZER Tapered, Glass, Interchangeable Highly efficient, all glass, tapered tissue grinder combining two grinding surfaces on a single pestle and tube. The conical area is for initial grinding and the cylindrical area for final homogenization. Interchangeably ground to clearance. With pouring lip. Capacities represent volumes with pestle inserted. Tube, only Pestle, only Complete Qty. Qty. Qty TISSUE HOMOGENIZER Dounce, Two Glass Pestles Supplied with two pestles, one loose and one tight, to ensure dissociation of cells into fine particles with minimal damage to cell nuclei. Use the large-clearance pestle for initial reduction of soft tissue. Complete the homogenization with the small-clearance pestle. Particularly useful in enzyme studies where heat build-up must be avoided. Body O.D. x Height, Overall Length, 1 11 x x x x TISSUE HOMOGENIZER Potter-Elvehjem, PTFE Pestle Homogenization occurs as the sample and buffer are forced through the cylindrical portion of the mortar as the pestle is rotated downward. Mortar is unground and the pestle contact surface is PTFE, threaded onto a stainless steel shaft. Body O.D. x Height, Overall Length, 2 11 x x x x x x TISSUE HOMOGENIZER Potter-Elvehjem, Micro Micro size for extremely precise work. This unit is designed for delicate hand operation. A reservoir and pouring lip are incorporated into the design. Body O.D. x Overall Length, Height, x TISSUE HOMOGENIZER Tenbroeck, All-Glass All-glass Tenbroeck tissue grinders come with precision-made, interchangeable pestles and tubes. The hollow handle permits packing with ice. This unit also features an expanded reservoir and pouring lip. Designed for tissues such as liver, intestines and heart. Body O.D. x Height, Overall Length, 1 11 x x x x x

12 12 P.O. Box 688 Vineland, NJ Fax: TOLL-FREE: FAX: Ace Glass, Inc. All rights reserved. December 13, :07 AM