April 20 (Wed) ~ 22 (Fri), 2016

Transcription

1 MSK2016 International Meeting of the Microbiological Society of Korea April 20 (Wed) ~ 22 (Fri), 2016 Kimdaejung Convention Center, Gwangju, Korea Hosted by The Microbiological Society of Korea Co-organized by Collaborative Genome Program for Fostering New Post-Genome Industry Strategic Initiative for Microbiomes in Agriculture and Food Sponsored by BioPS Co.,Ltd. Celltrion Inc. CJ CheilJedang Daesang Corporation DYNEBIO INC. Greencross Gwangju Convention & Visitors Bureau Intelligent Synthetic Biology Center Korea Research Institute of Bioscience and Biotechnology Korea Yakult Macrogen MBcell Medytox RAONTECH Co., ltd TEam for ControL of NOROviral Foodborne Outbreaks The Korean Federation of Science and Technology Societies World Institute of Kimchi

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4 Timetable 4 Floor Plans 5 Scientific Program 6 Plenary Lectures 27 PL1 28 PL2 29 PL3 30 PL4 31 PL5 32 Symposium 33 S1 33 S2 41 S3 47 S4 55 S5 61 S6 67 S7 73 S8 79 S9 85 S10 91 S11 97 S S S S S S S Young Scientists' Forum 145 YS1 146 YS2 152 Graduates Students' Forum 159 Poster 169 The 5 th Microbiology Research Festival for High School Students 199 Exhibition 217 Author Index 229 The Microbiological Society of Korea Rm. 810 (New Bldg.), The Korea Science & Technology Center 22, Teheran-ro 7-gil, Gangnam-gu, Seoul 06130, Republic of Korea msk@msk.or.kr Tel: Fax: This work was supported by the Korean Federation of Science and Technology Societies Grant funded by the Korean Government. 3

5 2016 International Meeting of the Microbiological Society of Korea Timetable 4.20 (Wed) Convention Hall 1 Convention Hall 2 Convention Hall 3 12:00- Registration 12:15-12:30 Opening Ceremony (Hall 3) 12:30-14:30 S1 New Faces of Microbiomes In Agriculture and Food YS1 Young Scientists' Forum 14:30-14:40 Break time 14:40-15:25 PL1 Prof. Angelika Gründling (Hall 3) 15:25-15:30 Break time 15:30-17:30 S2 Current Progress in Molecular Genetics and Cell Biology S3 Multiple Interactions between Host and Gut Microbiota GS Graduates Students' Forum S4 Microbes Meet Radiation: Gene to Industry 17:30-17:40 Break time 17:40-18:25 PL2 Prof. Oded Beja (Hall 3) 18:30-20:00 Reception with Poster Presentation 1 (Convention Hall Lobby, Sponsored by BioPS Co. Ltd) 4.21 (Thu) Convention Hall 1 Convention Hall 2 Convention Hall 3 Rm :00-11:00 S5 Molecular Microbiology of Pseudomonas aeruginosa S6 Omics Approaches in Systems Microbiology S7 Evolution and Ecology of Lichens S8 Probiotics and Gut Homeostasis 11:00-11:10 Break time 11:10-11:55 PL3 Prof. Aaron P. Mitchell (Hall 3) 11:55-13:30 LUNCH Poster Presentation 2 General Meeting of MSK 13:30-15:30 S9 Molecular Pathogenesis of Bacterial Pathogens S10 Ecophysiology of Environmentally Important Microorganisms 15:30-15:40 Break time 15:40-17:40 S13 Human Opportunistic Fungal Pathogens S14 Synthetic Microbiology for Biotechnology and Therapeutic Applications S11 Lactic Acid Bacteria and Fermented Foods S15 Ecological Aspect of Microorganisms via Phylogenetic Approach 17:40-17:50 Break time 17:50-18:35 PL4 Prof. Dirk Bumann (Hall 3) 18:40-20:30 Welcome Reception (Multi-purpose Auditorium) 4.22 (Fri) Convention Hall 1 Convention Hall 2 Convention Hall 3 S17 S18 S19 09:00-11:00 Signal Transduction and Gene Recent Advances in Deepsea Test and Research in Regulation in Bacteria and Extremophilic LMO Safety Management Microbiology 11:00-11:10 Break time 11:10-11:55 PL5 Prof. Bruno Lemaitre (Hall 3) 11:55-13:00 LUNCH 13:00-15:00 Bio-Company Session Advanced Technology for Microbiology YS2 Young Scientists' Forum 15:00-15:30 Closing Ceremony (Hall 3) HS High School Students' Presentation Session (12:00-14:00) Special Lecture (14:00-14:40) Award Presentation to High School Students (14:40-15:00) S12 Virus and Cancer S16 Recent Progress in Vaccine Development against Traditional and Emerging Pathogens 4

6 Floor Plans Convention Hall I Symposia, Bio-company Session Convention Hall II Symposia, Young Scientists' Forum 1 & 2 Convention Hall III Convention Hall Lobby Room Opening Ceremony, Plenary Lecture, Symposia, Graduates Students' Forum, General Meeting of MSK, Special Lecture, Closing Ceremony Registration Desk, Exhibition, Poster Presentation Symposia on April 21 (Thu) Poster Presentation Layout Zone Poster Presentation 1 Poster Presentation 2 1 B001-B014 / G046, G047 / H024-H035 A001-A014 / H022-H035 2 B015-B024 A015-A026 3 B025-B040 A027-A041 4 B041-B048 A042, A043 / D001-D006 5 B049-B053 / C001-C009 D007-D022 6 C010-C027 / E001-E012 D023-D055 7 E013-E026 / G001-G006 D056, D057 / F001-F020 8 G007-G021 F021, F022 / H011-H013 9 G022-G045 H014-H021 / HS1-HS18 5

7 2016 International Meeting of the Microbiological Society of Korea Scientific Program Plenary Lectures PL1 Plenary Lecture 1 April 20 (Wed), Convention Hall III Chair : Hyon E. Choy (Chonnam National University) 14:40-15:25 c-di-amp Targets Both Arms of Osmoprotection - Potassium and Osmolyte Uptake Systems Angelika Gründling (Imperial College London, UK) PL2 Plenary Lecture 2 April 20 (Wed), Convention Hall III Chair : Jung-Hyun Lee (KIOST) 17:40-18:25 Metagenomics of Light Harvesting in the Marine Environment Oded Beja (Technion-Israel Institute of Technology, Israel) PL3 Plenary Lecture 3 April 21 (Thu), Convention Hall III Chair : Yong-Sun Bahn (Yonsei University) 11:10-11:55 Gene Expression and Function in Candida albicans Infection Biology Aaron P. Mitchell (Carnegie Mellon University, USA) PL4 Plenary Lecture 4 April 21 (Thu), Convention Hall III Chair : Joon Haeng Rhee (Chonnam National University) 17:50-18:35 Salmonella Single-cell Dynamics in Complex Host Tissues Dirk Bumann (University of Basel, Switzerland) PL5 Plenary Lecture 5 April 22 (Fri), Convention Hall III Chair : You-Hee Cho (CHA University) 11:10-11:55 The Drosophila Antimicrobial Response at the Time of the Cas9/CRISPR Gene Targeting Revolution Bruno Lemaitre (Global Health Institute, Switzerland) 6

8 Special Lecture SL Special Lecture for Future Scientists April 22 (Fri), Convention Hall III 좌장 : 김인섭 ( 한남대학교 ) 14:00-14:40 활과리라 : 생물학과철학의접점찾기김응빈 ( 연세대학교 ) Symposium S1 New Faces of Microbiomes in Agriculture and Food April 20 (Wed), Convention Hall I Co-organized by Strategic Initiative for Microbiomes in Agriculture and Food Chair : Che Ok Jeon (Chung-Ang University) S1-1 12:35-12:55 Industrialization of Mycopesticdes to Control Frankliniella occidentalis for Integrated Thrips Management Jae Su Kim (Chonbuk National University) S1-2 12:55-13:15 Effect of Lactobacillus rhamnosus BFE5264 on Cholesterol Level and Gut Microbiota in High-cholesterol Diet-fed Mice Ji-hee Kang (Atogen Co. Ltd.) S1-3 13:15-13:35 Influence of Probiotics on Host Gut Microbiota and Patho-physiological Symptoms in a Diet Induced Obesity Murine Model Wilhelm H. Holzapfel (Handong Global University) S1-4 13:35-13:55 Metabolomics Based Interpretation of Microbial Fermentation Choong Hwan Lee (Konkuk University) 7

9 2016 International Meeting of the Microbiological Society of Korea S1-5 13:55-14:15 Keratin Degradation by Fervidobacterium islandicum AW-1 Dong-Woo Lee (Kyungpook National University) S1-6 14:15-14:35 Stress, Nutrition and Immune Regulation in Pigs Cheol-Heui Yun (Seoul National University) S2 Current Progress in Molecular Genetics and Cell Biology April 20 (Wed), Convention Hall I Chair : Won Hee Jung (Chung-Ang University) S2-1 15:30-16:00 Genetic Crosstalk between DNA Damage Repair Pathways and Oxidative Stress Responses for Maintenance of Genome Stability Woo-Hyun Chung (Duksung Women's University) S2-2 16:00-16:30 Rim11, the Human GSK-3β Homolog Is Involved in Replication Stress Response in Yeasts Yeon-Soo Seo (KAIST) S2-3 16:30-17:00 DNA Repair in Mycobacteria Umesh Varshney (Indian Institute of Science, India) S2-4 17:00-17:30 Bacterial Epi-metagenomics: Measuring DNA Methylation Patterns in Bacterial Community Level Woo Jun Sul (Chung-Ang University) 8

10 S3 Multiple Interactions between Host and Gut Microbiota April 20 (Wed), Convention Hall II Co-organized by Collaborative Genome Program for Fostering New Post-Genome Industry Chair : Heenam Stanley Kim (Korea University) S3-1 15:30-15:55 Development YOBMT for Improving Metabolic/Immune Disorders Jin-Woo Bae (Kyung-Hee University) S3-2 15:55-16:20 Gut Microbiota; the Potential Link to Rheumatic Diseases Seung Cheol Shim (Chungnam National University Hospital) S3-3 16:20-16:45 An Updated Evaluation of Next Generation Sequencing and Bioinformatics for Microbiome Analysis Jongsik Chun (Seoul National University & ChunLab, Inc.) Chair : Myung Hee Kim (KRIBB) S3-4 16:45-17:10 Comparative Swine Fecal Microbiota Analysis Across Breeds, Regions, Growth Stages, and Antibiotics Feed Additives Tatsuya Unno (JeJu National University) S3-5 17:10-17:35 Changes in the Swine Fecal Microbiota by the Administration of Probiotics and Prebiotics Dae-Kyung Kang (Dankook University) S4 Microbes Meet Radiation: Gene to Industry April 20 (Wed), Convention Hall III Sponsored by Korea Atomic Energy Research Institute Chair : Sung-Kee Jo (Korea Atomic Energy Research Institute) S4-1 15:30-16:00 Analysis of Deinococcus deserti : Species-specific Characteristics and Improved Characterization of Radioresistance in the Deinococcaceae Arjan de Groot (The French Alternative Energies and Atomic Energy Commission, France) 9

11 2016 International Meeting of the Microbiological Society of Korea S4-2 16:00-16:30 Enhanced Stress Tolerance of Escherichia coli Expressing Deinococcal Genes Sangyong Lim (Korea Atomic Energy Research Institute) S4-3 16:30-17:00 Single-molecule DNA Damage Analysis in E. coli Kyubong Jo (Sogang University) S4-4 17:00-17:30 Development and Evaluation of Irradiated Bacterial Vaccine Ho Seong Seo (Korea Atomic Energy Research Institute) S5 Molecular Microbiology of Pseudomonas aeruginosa April 21 (Thu), Convention Hall I Chair : You-Hee Cho (CHA University) S5-1 09:00-09:30 Regulation of Anthranilate Metabolism and Its Effect on Biofilm Formation in Pseudomonas aeruginosa Joon-Hee Lee (Pusan National University) S5-2 09:30-10:00 The Role of Pseudomonas aeruginosa DesB on Virulence Traits and Staphylococcus aureus Growth Inhibition in the Same Ecological Niche Kyoung-Hee Choi (Wonkwang University) S5-3 10:00-10:30 Making Pseudomonas aeruginosa Sensitive to Lysozyme Sang Sun Yoon (Yonsei University) 10

12 S5-4 10:30-11:00 Secreted Factors of Pseudomonas aeruginosa for the Modulation of Innate Immune Responses Un-Hwan Ha (Korea University) S6 Omics Approaches in Systems Microbiology April 21 (Thu), Convention Hall II Chair : Jong Hyun Choi (KRIBB) S6-1 09:00-09:30 In Pursuit of High-quality Reference Genomes: Causes of Failed Illumina Assemblies of Microbial Genomes and Their Improvements Haeyoung Jeong (KRIBB) S6-2 09:30-10:00 Understanding Virulence Regulation of Salmonella Typhimurium Using Proteomic Profiling of Outer Membrane Vesicles Hyunjin Yoon (Ajou University) S6-3 10:00-10:30 Computational Genomics Approach for Elucidation of Core Genes in Agarolytic Pathway In-Geol Choi (Korea University) S6-4 10:30-11:00 Transcriptome and Network Analysis of Butanol Stress in Escherichia coli Sung Ho Yoon (Konkuk University) S7 Evolution and Ecology of Lichens April 21 (Thu), Convention Hall III Chair : Soon Gyu Hong (KOPRI) S7-1 09:00-09:30 Diversity of Symbiotic Microalgae in Lichens Chae Haeng Park (KOPRI) 11

13 2016 International Meeting of the Microbiological Society of Korea S7-2 09:30-10:00 Comparative Genome Analysis of Lichen-forming Fungi and Partner Algae Sook-Young Park (Sunchon National University) S7-3 10:00-10:30 Pannariaceae Lichens: Model Organisms for Global Evolutionary Patterns Arve Elvebakk (University of Tromsø, Norway) S7-4 10:30-11:00 Recent Progress of Korean Lichen Biodiversity Survey: Korea National Arboretum Project Jae-Seoun Hur (Sunchon National University) S8 Probiotics and Gut Homeostasis April 21 (Thu), Room Sponsored by Korea Yakult Chair : Dae-Kyung Kang (Dankook University) S8-1 09:00-09:30 Characterization of Weissella cibaria Plasmid and Construction of Weissella Minimal Shuttle/Expression Vector Ju-Hoon Lee (Kyung Hee University) S8-2 09:30-10:00 Approaches to the Microbial Ecology of Food Systems Gisèle LaPointe (University of Guelph, Canada) S8-3 10:00-10:30 Rationally Selected Probiotics for Hyper-immune Disorders Sin-Hyeog Im (Institute for Basic Science & Pohang University of Science and Technology) 12

14 S8-4 10:30-11:00 Application of Nutrition Related-metabolomics for Attenuating Metabolic Diseases Hyeon Yeong Ahn (Yonsei University) S9 Molecular Pathogenesis of Bacterial Pathogens April 21 (Thu), Convention Hall I Sponsored by Korea Research Institute of Bioscience and Biotechnology Chair : Sang Ho Choi (Seoul National University) S9-1 13:30-14:00 Vibrio vulnificus RtxA1 Toxin as a New Target for Therapy Joon Haeng Rhee (Chonnam National University) S9-2 14:00-14:30 Vibrio MARTX Toxins Act as Effector Delivery Platforms to Inhibit Cellular Signaling during Infection Karla J.F. Satchell (Northwestern University Feinberg School of Medicine, USA) S9-3 14:30-15:00 Structural Architecture of Multidrug Efflux Pumps and Type I Secretion Systems from Gram-negative Bacteria Nam-Chul Ha (Seoul National University) S9-4 15:00-15:30 Horizontal Gene Transfer in Evolution of Mycobacterium tuberculosis towards Pathogenicity Olivier Neyrolles (Institute of Pharmacology and Structural Biology, France) S10 Ecophysiology of Environmentally Important Microorganisms April 21 (Thu), Convention Hall II Chair : Hee-Deung Park (Korea University) S :30-14:00 Real-Time Detection and Sorting of Colorful Microbes Using Raman Microspectroscopy Tae Kwon Lee (Yonsei University) 13

15 2016 International Meeting of the Microbiological Society of Korea S :00-14:30 Elucidating the Activity of Anaerobic Debrominating Bacteria: From Marine Sponges to Contaminated Sediments Max Häggblom (Rutgers, The State University of New Jersey, USA) S :30-15:00 Subsurface Microbial Redox Activities on Radionuclides in Contaminated Sediments Ji-Hoon Lee (Chonbuk National University) S :00-15:30 Searching for Core Microbiome in Wastewater Treatment Plant Bioreactors Hee-Deung Park (Korea University) S11 Lactic Acid Bacteria and Fermented Foods April 21 (Thu), Convention Hall III Sponsored by World Institute of Kimchi Chair : Ju-Hoon Lee (Kyung Hee University) S :30-14:00 Production of Lactobacillus brevis WK12, a Microbial Additive for Kimchi Fermentation, and Studies on Its Formulation Strategy Using Soy Powder and Microencapsulation Hae Woong Park (World Institute of Kimchi) S :00-14:30 Metabolomics Understanding of Lactic Acid Bacteria during Fermentation Young-Shick Hong (Chonnam National University) S :30-15:00 Health Claims for Probiotics and Prebiotics New Insights for Human Intervention Study to Measure Gut Inflammatory Response Ji Yeon Kim (Seoul National University of Science and Technology) 14

16 S :00-15:30 Comparison of NGS Platforms for the Analysis of Korean Gut Microbiome Young-Do Nam (Korea Food Research Institute) S12 Virus and Cancer April 21 (Thu), Room Chair : Seung-Min Yoo (Eulji University) S :30-14:00 Mechanism of KSHV Latency and Cellular Transformation Shou-Jiang Gao (University of Southern California, USA) S :00-14:30 New Insights into the Biology of Hepatitis B Virus: Viral Oncogenesis Wang-Shick Ryu (Yonsei University) S :30-15:00 IFN-λ4 Potently Induces ISG15/USP18-mediated IFN-ɑ Unresponsiveness Eui-Cheol Shin (KAIST) S :00-15:30 Constitutive Activation of T Cells by a Herpesviral GPCR through the Interaction with Cellular CXCR4 Nam-Hyuk Cho (Seoul National University) S13 Human Opportunistic Fungal Pathogens April 21 (Thu), Convention Hall I Sponsored by BK21PLUS Initiative for Biological Function & Systems Chair : Hyun Ah Kang (Chung-Ang University) S :40-16:10 Dimorphism and Host-Pathogen Interactions in the Emerging Fungal Infection, Mucormycosis Soo Chan Lee (Duke University Medical Center, USA) 15

17 2016 International Meeting of the Microbiological Society of Korea S :10-16:40 Systematic Functional Analysis of Pathogenicity Networks in a Global Fungal Meningitis Pathogen Yong-Sun Bahn (Yonsei University) S :40-17:10 Synthesis and Regulation of Zearalenone in Fusarium graminearum Yin-Won Lee (Seoul National University) S :10-17:40 The NDR Kinase Cbk1: A Versatile Player in the Hyphal Morphogenesis of Candida albicans Jeong-Yoon Kim (Chungnam National University) S14 Synthetic Microbiology for Biotechnology and Therapeutic Applications April 21 (Thu), Convention Hall II Chair : Yeo Joon Yoon (Ewha Womens University) and Gyoo Yeol Jung (POSTECH) S :40-16:10 Microbial Cell Factory for Isoprenoids Production Seon-Won Kim (Gyeongsang National University) S :10-16:40 Evolutionary Engineering for Chemical Producing Microorganisms Using Synthetic Regulators Gyoo Yeol Jung (Pohang University of Science and Technology) S :40-17:10 Bacteria-mediated Cancer Theranostics: Bacteria Meet Oncology Jung-Joon Min (Chonnam National University) 16

18 S :10-17:40 Engineered Biosynthesis of Non-immunosuppressive FK566 Analogues with Improved Therapeutic Properties Yeo Joon Yoon (Ewha Womans University) S15 Ecological Aspect of Microorganisms via Phylogenetic Approach April 21 (Thu), Convention Hall III Sponsored by CFST, Seoul National University Chair : Kae Kyoung Kwon (KIOST) S :40-16:10 Systematics of Vibrionaceae Based on Analysis of Genome Sequence Data Henryk Urbanczyk (University of Miyazaki, Japan) S :10-16:40 Microbiome Studies in Various Samples Bong-Soo Kim (Hallym University) S :40-17:10 Characterization of Extremely Halophilic Microbial Eukaryotes (Protozoa): Autecology and Diversity Jong Soo Park (Kyungpook National University) S :10-17:40 Platforms for Analysis of Fungal Diversity and Application to the Antarctic Environments Soon Gyu Hong (KOPRI) 17

19 2016 International Meeting of the Microbiological Society of Korea S16 Recent Progress in Vaccine Development against Traditional and Emerging Pathogens April 21 (Thu), Room Sponsored by International Vaccine Institute Chair : Man Ki Song (IVI) and Jae-Ouk Kim (IVI) S :40-16:10 Universal Influenza Vaccine Approach: Options and Obstacles Baik-Lin Seong (Yonsei University) S :10-16:40 Human DPP4 Transgenic Mice as Surrogate Models for MERS Chien-Te Kent Tseng (University of Texas Medical Branch, USA) S :40-17:10 In Vivo Molecular Imaging Analysis of Vaccine Candidates in a Mouse Model Hyewon Youn (Seoul National University Hospital) S :10-17:40 Vaccine Development Program for Developing Countries, International Vaccine Institute Man Ki Song (International Vaccine Institute) S17 Signal Transduction and Gene Regulation in Bacteria April 22 (Fri), Convention Hall I Chair : Jung-Hye Roe (Seoul National University) S :00-09:30 H 2O 2 Sensitivity of Metal-dependent Peroxide Sensor PerR Jin-Won Lee (Hanyang University) 18

20 S :30-10:00 'Oxygen-FNR-sRNA Regulatory Pathway' Controls the Anaerobic Induction of Fermentation-Respiration Switch Protein Kyu-Ho Lee (Sogang University) S :00-10:30 Three Mechanisms of Oxygen Sensing in Mycobacteria: Regulation of Gene Expression in Response to Changes in Oxygen Tensions Jeong-Il Oh (Pusan National University) S :30-11:00 Multiple Transcription Regulators of OhrR Family Responsible for Regulatory Cross Talk and Sequential Graded Gene Expression in Response to Organic Hydroperoxides in Agrobacterium tumefaciens Skorn Mongolsuk (Mahidol University, Thailand) S18 Recent Advances in Deepsea and Extremophilic Microbiology April 22 (Fri), Convention Hall II Chair : Jang-Cheon Cho (Inha University) S :00-09:30 Microbial Diversity, Biotechnological Potential and Adaptation to Deep Sea Hydrothermal Vents Conditions Mohammed Jebbar (Université de Bretagne Occidentale, France) S :30-10:00 Hydrogen Peroxide Detoxification: Physiological and Ecological Implications for Marine Ammonia-oxidizing Archaea Sung-Keun Rhee (Chungbuk National University) S :00-10:30 DNA Backbone Modification Expands Microbial Growth Range under Multiple Stresses Xiang Xiao (Shanghai Jiao Tong University, P.R. China) 19

21 2016 International Meeting of the Microbiological Society of Korea S :30-11:00 Anaerobic Oxidation of Methane at the SMTZ of the Deep Sediments of Ulleung Basin, East Sea of Korea Jung-Hyun Lee (KIOST) S19 Test and Research in LMO Safety Management April 22 (Fri), Convention Hall III Sponsored by Korea Research Institute of Bioscience and Biotechnology Chair : Sang Jun Lee (KRIBB) S :00-10:00 LMO Laws and Regulations in Korea In-Ja Song (KRIBB) S :00-11:00 Safety Management of LMO Facilities Kyung-Hwa Choi (KRIBB) Young Scientists Forum YS1 Young Scientists Forum 1 April 20 (Wed), Convention Hall II Chair : Jang-Cheon Cho (Inha University) YS1-1 12:30-12:50 Biocontrol of Staphylococcus aureus and Pectobacterium carotovorum by Bacteriocins and Application for Food Safety Jonguk Kim (Rural Development Administration) YS1-2 12:50-13:10 Source Tracking and Succession of Kimchi Lactic Acid Bacteria during Fermentation Se Hee Lee (World Institute of Kimchi) YS1-3 13:10-13:30 Oxidative Stress Response in Acinetobacter oleivorans DR1 Jisun Kim (Korea University) 20

22 YS1-4 13:30-13:50 Expansion of Cultured Bacterial Diversity by Large-scale Dilution-to-Extinction Culturing from a Single Seawater Sample Seung-Jo Yang (National Marine Biodiversity Institute of Korea) YS1-5 13:50-14:10 A Machine Learning Approach for Prediction of in situ Chlorinated Ethene Dechlorination Potential Jaejin Lee (Korea Polar Research Institute) YS1-6 14:10-14:30 Isolation and Ecophysiological Characterization of a Polycyclic Aromatic Hydrocarbon-degrading Bacterium, Alteromonas naphthalenivorans SN2, from a Contaminated Tidal Flat Hyun Mi Jin (Nakdonggang National Institute of Biological Resources) YS2 Young Scientists Forum 2 April 22 (Fri), Convention Hall II Chair : Sung Ho Yoon (Konkuk University) YS2-1 13:00-13:20 Killing Vibrio cholerae by a Chemical Modulator for Glucose Metabolism Young Taek Oh (Yonsei University) YS2-2 13:20-13:40 Improved Production of Immunosuppressant Rapamycin Young Ji Yoo (Ewha Womans University) YS2-3 13:40-14:00 The Elongation Factor P Regulate Expression of Magnesium Transport Protein in Salmonella Typhimurium Eunna Choi (Kyung Hee University) 21

23 2016 International Meeting of the Microbiological Society of Korea YS2-4 14:00-14:20 The Effect of Rsd, the Anti-sigma Factor of σ 70, on Biofilm Formation and Motility in Escherichia coli Young-Ha Park (Seoul National University) YS2-5 14:20-14:40 Role of Hepcidin in Salmonella Infection Jae-Ho Jeong (Chonnam National University Medical School) YS2-6 14:40-15:00 Rad53 Regulates Genotoxic DNA Damage Stress and Radiation Resistance through Mrr1 Transcription Factor in C. neoformans Kwang-Woo Jung (Korea Atomic Energy Research Institute) Graduate Students Forum GS Graduate Students Forum April 20 (Wed), Convention Hall III Chair : Yoonkyung Park (Chosun University) GS-1 12:30-12:45 Mycosphere of Tricholoma matsutake (Pine Mushroom): Its Impact and Role Seung-Yoon Oh (Seoul National University) GS-2 12:45-13:00 The Effect of Viscosity on Predation by Bdellovibrio bacterivorus HD100 Hansol Im (Ulsan National Institute of Science and Technology) GS-3 13:00-13:15 Renewable Production of 1,3-Diaminoporpane (A Three Carbon Diamine) by Metabolically Engineered Escherichia coli Tong Un Chae (KAIST) GS-4 13:15-13:30 Identification and Regulatory Characteristics of Vibrio vulnificus plp Encoding a Phospholipase Essential for Pathogenesis Kyung Ku Jang (Seoul National University) 22

24 GS-5 13:30-13:45 Salmonella Virulence Protein Activates Sugar Phosphate Uptake Jang-Woo Lee (Kyung Hee University) GS-6 13:45-14:00 The Ferrichrome Receptor A as a New Target for Pseudomonas aeruginosa Virulence Management Keehoon Lee (Yonsei University College of Medicine) GS-7 14:00-14:15 Immunization of 13 Amino Acid Peptide Targeting Srr Proteins Provide a Broad Spectrum of Protections Against Group B Streptococcal Infections Shunmei Lin (Korea Atomic Energy Research Institute) GS-8 14:15-14:30 Cps35/Swd2 Mediates the Histone Crosstalk between H2B Ubiquitination and H3K4 Methylation Shinae Park (Kangwon National University) Bio-company Session BC Advanced Technology for Microbiology April 22 (Fri), Convention Hall I Chair : Se-Jong Oh (Chonnam National University) BC-1 13:00-13:20 Introduction of Cedex Systems in Advanced Technology Anne Lee (Roche Diagnostics Korea) BC-2 13:20-13:40 Single Use Technology for Bioprocess Chan Jun Moon (Sartorius Korea Biotech Co., Ltd.) BC-3 13:40-14:00 ViroMed, a Leader in the Development of New and Innovative Biopharmaceuticals Seungshin Yu (ViroMed Co., Ltd.) BC-4 14:00-14:20 RNAi Oligo Therapeutics as a Next Generation Drug Joo-Sung Yang (Bioneer Corporation) BC-5 14:20-14:40 Research with Monoclonal Antibody: A Future Direction To Go Bum-Chan Park (A&RT) BC-6 14:40-15:00 Designers Enzyme GenoFocus Taekho Yang (GenoFocus) 23

25 2016 International Meeting of the Microbiological Society of Korea 제 5 회미생물탐구페스티벌 The 5 th Microbiology Research Festival for High School Students HS High School Students Presentation Session April 22 (Fri), Convention Hall III 12:00-12:10 개회 좌장 : 한국기초과학지원연구원최종순박사 HS-1 12:10-12:20 Sporosarcina pasteurii 유전자도입형질전환토양세균을이용한토양내수분보유능력개선및식물생장촉진효과박상윤 (Deerfield Academy) HS-2 12:20-12:30 청색광에서의 IAA (Indol 3-Acetate Acid) 분해및활성산소소거능에의한땀세균억제효과이승은 ( 서울국제학교 ) HS-3 12:30-12:40 식물공장의세균과곰팡이에대한오염을해결하기위한방안에대하여천승재 (Oakridge Secondary School) HS-4 12:40-12:50 옻나무 (Rhus trichocarpa) 와자작나무 (Betula platyphylla var. japonica) 추출물의항균활성및천연보존료개발가능성탐구이수민, 김윤정 ( 이사벨고등학교 ) HS-5 12:50-13:00 로돕신발현으로인공적인광영양성을획득한대장균의실험실내적응진화김현 ( 하나고등학교 ) HS-6 13:00-13:10 애벌레를이용한플라스틱분해세균연구김현아, 김하연 ( 하나고등학교 ) HS-7 13:10-13:20 밭수확량증대를위한미생물생장에최적화된석회비료개발김다솔, 김지윤, 류혜지, 임재웅 ( 경산과학고등학교 ) HS-8 13:20-13:30 토양미생물분석을통한아미그달린분해능탐구박재희, 윤도현, 김민석, 박시현 ( 광주과학고등학교 ) 24

26 HS-9 13:30-13:40 HS-10 13:40-13:50 토양의종류에따른토양세균의탈염기능분석과이를적용한음식물쓰레기친환경퇴비개발에관한연구 정진운 ( 한성과학고등학교 ) 자생지별로분리된춘란의난근균근 (Orchid Mycorrihizal Fungi) 이병원균과자생지에서식하는토양미생물과춘난뿌리에주는영향 남윤성 ( 홍천고등학교 ) 25

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28 Plenary Lectures

29 2016 International Meeting of the Microbiological Society of Korea PL-1 c-di-amp Targets Both Arms of Osmoprotection - Potassium and Osmolyte Uptake Systems Lauren Schulte 1, Christopher F. Schuster 1, Tommaso Tosi 1, Ivan Campeotto 1, Rebecca M. Corrigan 1, Paul Freemont 2, and Angelika Gründling 1 * 1 Section of Microbiology, Imperial College London, UK 2 Section of Structural Biology, Imperial College London, UK Cyclic diadenosine monophosphate (c-di-amp) is an essential second messenger in Staphylococcus aureus but it physiological function remains enigmatic. In a previous high throughput screen, four c-di-amp receptor proteins were identified: a P II like protein of unknown function, a putative cation/ proton antiporter, a gating component of a potassium uptake system, and a protein involved in the regulation of a second potassium transport system. The current study revealed an additional c-di-amp binding protein, named OpuCA, a substrate-binding component of an osmoprotectant ABC uptake system. Physiological tests indicate that the OpuC system plays a role in osmoprotection through the uptake of the compatible solute carnitine. The two main mechanisms, which bacteria utilize to respond to osmotic stress, are the rapid uptake of potassium and osmolytes. With the identification of OpuCA as a novel c-di-amp binding protein, we now linked this signaling molecule to both arms of osmoprotection. This points towards c-di-amp being a general regulator of the osmotic stress response in S. aureus. 28

30 Plenary Lectures PL-2 Metagenomics of Light Harvesting in the Marine Environment Oded Beja Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel Environmental-Genomics / Metagenomics is an emerging field that enables us to look at parts of the ocean that were, until recently, masked to us. With present estimates suggesting that >99% of the microorganisms in most environments are not amenable to growth in pure culture, very little is known about their physiology and roles in the ocean. These organisms can, however, be categorized into phylotypes according to their ribosomal RNA (rrna) genes, which can be amplified directly from environmental DNA extracts, cloned, and sequenced. Although this approach has provided information on the identity and distribution of microbial species, rrna gene sequences alone do not reveal the physiology, biochemistry, or ecological function of uncultivated microorganisms. This problem can be bypassed by accessing the genomes of these microorganisms and identifying protein coding genes and biochemical pathways that will shed light on their physiological properties and ecological function. To illuminate the role of microorganisms in the open seas, my lab is exploring the metabolism of planktonic microbes using novel molecular biology techniques, along with functional genomics and bioinformatics. The lab is now focusing on photosynthesis genes found in viruses that infect cyanobacteria and on developing different functional metagenomic screens. In my talk I will discuss the instrumental use of metagenomics in the discovery of marine microbial rhodopsins and viral photosystems. 29

31 2016 International Meeting of the Microbiological Society of Korea PL-3 Gene Expression and Function in Candida albicans Infection Biology Wenjie Xu 1, Norma V. Solis 2, Rachel L. Ehrlich 1, Carol A. Woolford 1, Scott G. Filler 2 *, and Aaron P. Mitchell 1 * 1 Department of Biological Sciences, Carnegie Mellon University, USA 2 Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, USA We have analyzed expression of environmentally responsive genes and transcription factor genes to infer signals and pathways that drive pathogen gene regulation during invasive Candida albicans infection of a mammalian host. Both sets of genes reveal both early and late phases of infection. The early phase includes induction of zinc and iron limitation genes, genes that respond to transcription factor Rim101, and genes characteristic of invasive hyphal cells. The late phase includes responses related to phagocytosis by macrophages. Transcription factor genes that are required for virulence or proliferation in vivo are enriched among highly expressed transcription factor genes. Mutants defective in six transcription factor genes, three previously studied in detail (Rim101, Efg1, Zap1) and three less extensively studied (Rob1, Rpn4, Sut1), are profiled during infection. Most of these mutants have distinct gene expression profiles during infection as compared to in vitro growth. Infection profiles suggest that Sut1 acts in the same pathway as Zap1, and we verify that functional relationship with the finding that overexpression of either ZAP1 or the Zap1-dependent zinc transporter gene ZRT2 restores pathogenicity to a sut1 mutant. Our results reveal that the infection environment has drivers of gene expression that are distinct from in vitro growth conditions, and that unique functional genetic relationships emerge during infection. 30

32 Plenary Lectures PL-4 Salmonella Single-cell Dynamics in Complex Host Tissues Dirk Bumann Focal Area Infection Biology, Biozentrum, University of Basel, Switzerland Infectious diseases remain a major cause of death worldwide. Lack of efficacious vaccines against important pathogens, and rapidly rising antimicrobial resistance pose a major threat to human health. Infection research has discovered many host-pathogen interactions, but most in vivo studies use bulk average readouts that cannot capture the vast and dynamic complexity of infected tissues. We have recently developed single-cell techniques that report on survival/killing, stress exposure, and growth rates of individual Salmonella cells in infected mouse tissues. In addition, we are currently developing 3D imaging approaches to localize individual Salmonella in tissue microenvironments at mm to nm scale. Our results show that after oral infection, individual Salmonella arrive from gut-associated tissues at various sites throughout the entire spleen. Distinct microenvironments expose Salmonella to widely different levels of oxidative and nitrosative stress, resulting in massive death of Salmonella in inflammatory lesions, but successful local adaptation and stress defense of others. Differential nutrient supply causes a broad range of Salmonella growth rates and this is dependent on host cell phagosome structure and content. Fast growing subsets drive disease progression and form local infection foci. In contrast, moderately growing subsets contribute little to overall disease but are tolerant against antibiotics resulting in treatment failures. These results show that this salmonellosis consists of strikingly heterogeneous and dynamic Salmonella-host encounters involving diverse tissue regions, cell types, and molecular mechanisms. Overall disease progression results from failures in host control or antibiotic therapy at some tissue sites, despite successful simultaneous eradication in others. Disparate Salmonella-host encounters thus make the difference between lethal disease and successful control. 31

33 2016 International Meeting of the Microbiological Society of Korea PL-5 The Drosophila Antimicrobial Response at the Time of the Cas9/CRISPR Gene Targeting Revolution Bruno Lemaitre Global Health Institute, Ecole Polytechnique Fédérale of Lausanne, Lausanne, Switzerland The application of Drosophila genetics to these mechanisms has generated insights into insect immunity and uncovered general principles of animal host defense. These studies have shown that Drosophila has multiple defense modules that can be deployed in a coordinated response against distinct pathogens. Today, Drosophila can be considered as having one of the best-characterized host defense systems among the metazoan. Until recently, a detailed understanding of the fly immune response was hampered by the difficulty of generating loss-of-function mutations as well as the technological limits of the RNAi approach. The Cas9/CRISPR revolution offers new opportunities to revisit in a systematic manner Drosophila immunity. At the interface between large-scale genomic studies that lack resolution and individual gene analysis that lack breadth, our laboratory has undertaken a meso-scale skilled analysis of immune modules, notably by addressing the individual and overlapping function of large immune gene family. In this talk, I will summarize our current knowledge of the field and provide new insights recently gained in the laboratory. 32

34 Symposium [S1] New Faces of Microbiomes in Agriculture and Food Co-organized by Strategic Initiative for Microbiomes in Agriculture and Food

35 2016 International Meeting of the Microbiological Society of Korea S1-1 Industrialization of Mycopesticdes to Control Frankliniella occidentalis for Integrated Thrips Management Jae Su Kim 1 *, Se Jin Lee 1, Sihyeon Kim 1, Jong Cheol Kim 1, Mi Rong Lee 1, Yu-Shin Nai 2, Yi-Ting Yang 1, Tae Hoon Kim 3, and Teak Soo Shin 3 1 Department of Agricultural Biology, Chonbuk National University 2 Department of Biotechnology and Animal Science, National Ilan University, Taiwan 3 AgroLife Research Institute, Dongbu Farm Hannong Co. Western flower thrips (WFT), Franklinella occidentalis, is a major pest of ornamentals. Mycotized millet grains with entomopathogenic fungi applied to soil of potted marigold plants was tested to target pupating thrips. Two experimental fungal isolates, (Beauveria bassiana [ARS7060] and Metarhizium anisopliae [ERL1171]), were compared with the registered B. bassiana strain GHA [commercialized as BotaniGard ] and untreated controls in greenhouse caged trials. Mycotized millet grains were mixed into the upper surface of the potting soil in pots of flowering Hero Yellow marigolds (4 g/pot). One week after application five mated WFT females were released onto each plant (four plants per cage). At 8 wks post-infestation, the mean total number of thrips per plant was 81 and 90% less in the ERL1171 and ARS 7060 treatments, respectively, than in the controls. The mean numbers of thrips per plant for the control and GHA treatments were not significantly different. Plant damage was 60% less on plants treated with the experimental fungi than the control and GHA treatments. At 10 wks post-application, 75 90% of WFT collected from the treatments were infected with the experimental isolates. These results demonstrate that soil applications of entomopathogenic fungi can reduce WFT populations significantly and prevent damage. 34

36 Symposium [S1] : New Faces of Microbiomes in Agriculture and Food S1-2 Effect of Lactobacillus rhamnosus BFE5264 on Cholesterol Level and Gut Microbiota in High-cholesterol Diet-fed Mice Ji-hee Kang 1 *, Wilhelm Holzapfel 2, Yosep Ji 2, and Hong-sup Yoon 2 1 Atogen Co. Ltd. 2 School of Life Sciences, Handong Global University Hypercholesterolaemia is a major risk factor related to atherosclerosis, and it may be influenced by our diet. Lactobacillus rhamnosus BFE5264, isolated from traditional fermented milk of African Maasai tribe, promoted cholesterol efflux in enterocytes by up-regulating LXR, concomitantly with the elevated expression of ABCG5 and ABCG8. Caco-2 cells showed lower Niemann-Pick C1-like 1 (NPC1L1) expression in the presence of Lactobacillus rhamnosus BFE5264, elucidationg down-regualtion of cholesterol uptake. In animal experiment using high cholesterol diet-administered mouse, Lactobacillus rhamnosus BFE5264 reduced cholesterol level in blood and liver and showed similar mechanism with that is shown in cell test. Lactobacillus rhamnosus BFE5264 also changed short chain fatty acids (SCFAs) concentration and composition in gut of tested animals. Moreover, this strain changed gut microbial compostion. The Clostridiaceae content of the cecal microbiota was significantly increased in the BFE5264 group relative to that of the control group, whereas the content in faeces was significantly decreased. Principal coordinates anlaysis (PCoA) showed distinct change of Sphingomonas genus in BFE5264 group. 35

37 2016 International Meeting of the Microbiological Society of Korea S1-3 Influence of Probiotics on Host Gut Microbiota and Patho-physiological Symptoms in a Diet Induced Obesity Murine Model Wilhelm H. Holzapfel*, Soyoung Park, and Yosep Ji Department of Advanced Green Energy and Environment, Handong Global University Probiotic lactobacilli are widely recognized for their beneficial health impact on the host, especially regarding their prominent bioactivity to modulate host gastrointestinal (GIT) microbiota and associated metabolites such as short chain fatty acids (SCFA). Extensive reports are contributing to an improved understanding of ways in which our GIT microbiota is interrelated with host patho-physiological conditions such as obesity, type two diabetes and various other immuno-metabolic disorders. Species such as Akkermansia muciniphila and Faecalibacterium prausnitzii appear to play a specific role in obesity and IBD. Still, from the rapidly accumulating data, definition of indicator microbial groups for describing patho-physiological symptoms appears difficult. Acknowledging GIT microbiota as a vulnerable and rather adjustable secondary organ, probiotic applications can be a promising approach to benefit host health by restoring the balance of microbiota. Using a high fat diet induced obesity (DIO) C57BL/6J murine model, we compared whole and active gut microbial communities using bacterial genomic DNA (gdna) and ribosomal RNA (rrna), respectively, in response to administration of a probiotic strain. Further biomarkers associated with patho-physiological symptoms of the DIO model were analysed to understand the functional impact of a probiotic on the host. Distinct aspects were monitored quantitatively and in terms of complexity, and possible correlation with host microbiota modulation was investigated. Specific groups of microbiota corresponded either positively, negatively or irregularly with the expression of various biomarkers of the host, as a result of probiotic administration. 36

38 Symposium [S1] : New Faces of Microbiomes in Agriculture and Food S1-4 Metabolomics Based Interpretation of Microbial Fermentation Choong Hwan Lee Department of Bioscience and Biotechnology, Konkuk University The metabolomics is the studies on metabolites, and by-products of the chemical reactions that continuously go on in every biological system. Metabolomics, the chemical profiling of (all) cellular metabolites by their identification and quantification, is a rapidly expanding strategy in the post-genomics era complementing transcriptomics and proteomics thereby constituting a trilogy. A searchable library of MS/MS spectra, obtained using a quadrupole ion trap mass spectrometer and electrospray ionization, is presented for metabolomic profiling of secondary metabolite. The application of wideband excitation and normalized collision energy leads to highly reproducible mass spectra which are searched using the NIST algorithm. The ability to obtain library searchable spectra is demonstrated for the analysis of 6,000 secondary metabolites spectrum data. This metabolomics study provides valuable information in regards to optimizing the fermentation process for bioactive compound production and describes an efficient way to search for novel bioactive compounds with primary and secondary metabolism. The combined mass spectrometry approaches used in this study may be useful in understanding the overall metabolism in the various microbes and fermentation including in vivo study. This presentation will show that a MS-based metabolomics approach is a powerful tool for chemotaxonomic classifying and gene function studies of fungi with in vivo metabolomics case model of bioactive compounds. 37

39 2016 International Meeting of the Microbiological Society of Korea S1-5 Keratin Degradation by Fervidobacterium islandicum AW-1 Dong-Woo Lee School of Applied Biosciences, Kyungpook National University To date several microorganisms are known to degrade native poultry feathers, but the degradation mechanism of keratin still remains unclear. Herein we physiologically characterized the extremely thermophilic anaerobe Fervidobacterium islandicum AW-1, that could degrade native chicken feathers at 70 C, and then sequenced the 2.23 Mb-genome of this bacterium. Subsequently, we performed transcriptome analysis by RNA-seq for F. islandicum AW-1 cells grown on native feathers versus glucose, and compared their subcellular proteomes by LC-MS/MS analysis. These data indicate that together with several proteases responsible for keratin degradation, specific sets of metabolic pathways involved in cofactor and vitamin biosynthesis, membrane biosynthesis, chemotaxis, and motility are highly correlated with this bacterial unique features at elevated temperatures. Therefore, this study provides insight into nature s utilization of recalcitrant protein polymers under extreme environments. 38

40 Symposium [S1] : New Faces of Microbiomes in Agriculture and Food S1-6 Stress, Nutrition and Immune Regulation in Pigs Cheol-Heui Yun 1,2 * and Byung-Chul Park 2,3 1 Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University 2 Institutes of Green Bio Science and Technology, Seoul National University 3 Department of International Agricultural Technology, Graduate School of International Agricultural Technology, Seoul National University With respect to fast growing and global changes of international atmosphere, stresses have been concerned for decades in livestock industry. Major stresses including heat, nutrition and infection could alter not only the growth performance, but also systemic and local immune system. It is also well known that major stresses impact on gut health. Heat stress (HS) increased the permeability and the inflammatory responses in the gut. Nutritional stresses, such as fasting or fed with mycotoxin contaminated feed, induced the destruction of the tight junction proteins in the gut. Fasting suppressed pro-inflammatory cytokines, whereas deoxynivalenol (DON) up-regulated the recruitment of intestinal pro-inflammatory cytokines and the level of lymphocytes in gut. Pigs infected with pathogens such as Enterotoxigenic E. coli (ETEC) and porcine epidemic diarrhea virus (PEDV) lead to loosen up the intestinal epithelial barrier. On the other hand, supplementation of Lactobacillus plantarum or Saccharaomyces cerevisiae boulardii reduced infectious stress by ETEC. It was noting that major stresses altered the permeability of the intestinal barriers and profiles of genes and proteins of pro-inflammatory cytokines and chemokines in porcine gut. However, it is not sufficient to fully explain the mechanism of gut immune system in pigs under stress condition. In near future, the interaction of gut and systemic immune system under major stresses should be defined precisely to overcome aforementioned obstacles. 39

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42 Symposium [S2] Current Progress in Molecular Genetics and Cell Biology

43 2016 International Meeting of the Microbiological Society of Korea S2-1 Genetic Crosstalk between DNA Damage Repair Pathways and Oxidative Stress Responses for Maintenance of Genome Stability Woo-Hyun Chung College of Pharmacy, Duksung Women s University A global genetic analysis of synthetic fitness or lethality (SFL) defect interactions in yeast revealed that mutations of five genes required for oxidative stress response (TSA1, SOD1, LYS7, SKN7, and YAP1) impaired growth of mutants in homologous recombination (HR) pathways and interestingly all these genes play a significant role in suppression of mutagenesis. SOD1 inhibition has been proposed as a promising approach to the selective killing of cancer cells and synthetic lethal interaction between yeast rad54 and sod1 is shown to be conserved within a human colorectal cancer (CRC). Pathways of DNA damage repair and oxidative stress responsive signaling have been proposed to be highly associated in the cell, but the underlying molecular mechanism remains unknown. We employed mutant strains lacking Rad51, the homolog of E. coli RecA recombinase, and Yap1 or Skn7, two major transcription factors responsive to reactive oxygen species (ROS), to examine genetic interactions between double-strand break (DSB) repair proteins and cellular redox regulators in budding yeast Saccharomyces cerevisiae. Abnormal expression of YAP1 or SKN7 aggravated spontaneous mutation rate and sensitivity of rad51 mutant to DSB- and ROS-generating reagents. Rad51 deficiency contributed to genome instability more in response to the increased ROS, and the accumulation of DSB lesions raised the intracellular ROS level. Our findings suggest that there is a significant crosstalk between DSB repair pathways and ROS signaling proteins for cell survival and maintenance of genome integrity in response to genotoxic stresses. 42

44 Symposium [S2] : Current Progress in Molecular Genetics and Cell Biology S2-2 Rim11, the Human GSK-3β Homolog Is Involved in Replication Stress Response in Yeasts Annie Albert Demin, Miju Lee, Chul-Hwan Lee, and Yeon-Soo Seo* Department of Biological Sciences, KAIST The GSK-3β kinase is linked to many kinds of cancer either as a tumor suppressor or as a tumor promoter. Genomic instability is one of the underlying hallmarks for tumor initiation. The link between GSK-3β and genomic instability is still unclear. In the perspective of tumor development and progression, mutations are believed to accumulate due to compromised DNA repair. Using yeast genetics as a powerful tool, we discovered Rim11, the human GSK-3β homolog as a suppressor of dna2 and rad18 mutants. Dna2 is an essential endonuclease/helicase in Okazaki fragments synthesis, whereas Rad18 is an E3 ubiquitin ligase responsible for activating the post-replication repair (PRR). Overexpression of Rim11 kinase suppressed the lethality of dna2 helicase-dead mutant and the methyl methane sulfonate (MMS) sensitivity of rad18 null mutant. The substrate for Rim11 responsible for the suppression is Ume6, a DNA binding protein. Ume6 interacts with the histone deacetylase complex (HDAC) Sin3/Rpd3, and this interaction and the deacetylase activity of Rpd3 are necessary for the Rim11-dependent suppression of dna2 mutant. Through epistatic analysis we showed that the Rim11-initiated suppression of dna2 and rad18 mutants promotes sister-chromatids recombination (SCR) mediated by the Rad52/Rad59 proteins. In support of this, both dna2 and rad18 mutants could be rescued by the overexpression of Rad52. Moreover, we found that checkpoint arrest is heavily enforced in dna2 mutant. Checkpoint could impose restriction on recombination-mediated repair. As previously reported Rpd3-mediated deacetylation of Rad53 facilitates in suppressing the activation of checkpoint. We asked whether the role of Rpd3 is to suppress checkpoint therefore allowing HR repair. Indeed, the removal of key checkpoint proteins like Rad9 or Rad53; or overexpression of ribonucleotide reductase inhibitor Sml1 allowed cells with dna2 mutation to be viable. Our data demonstrate that GSK-3β homolog Rim11 is directly involved in repair of faults in Okazaki fragment synthesis, by promoting homologous recombination by down-regulating checkpoint activation, revealing a novel pathway of post-replication repair (PRR) that is distinct from the well-described Rad6-Rad18 pathways. Our finding could account for why GSK-3β promote cell proliferation and tumor growth and explain why down regulating GSK-3β is beneficial in cancer therapy. The elevated levels of GSK-3β could allow tumor cells to easily overcome replicative stress by facilitating DNA repair in cancer. 43

45 2016 International Meeting of the Microbiological Society of Korea S2-3 DNA Repair in Mycobacteria Umesh Varshney Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India In the host macrophages, Mycobacterium tuberculosis is exposed to reactive oxygen and nitrogen species (ROS and RNI) produced as a part of the host s innate immune response. ROS and RNI are highly reactive and cause severe damages to DNA or the free nucleotide pools in the cell. Physiological roles of several DNA repair mechanisms such as the base excision and nucleotide excision repair pathways, and the ones involved in elimination of the oxidized nucleotides (7, 8-dihydro-8-oxoguanine, 8-oxoG or its derivatives) have been studied. More recently, we discovered a novel protein, UdgX, from Mycobacterium smegmatis and other organisms. UdgX specifically recognizes uracil in DNA, forms a tight complex stable to sodium dodecylsulphate, 2-mercaptoethanol, urea and heat treatment, and shows no detectable uracil excision. UdgX shares highest homology to family-4 uracil DNA glycosylase (UDG) possessing Fe-S cluster. UdgX possesses a conserved sequence, KRRIH, which forms a flexible loop playing an important role in its activity. Mutations of H in the KRRIH sequence to S, G, A or Q lead to gain of uracil excision activity in MsmUdgX, establishing it as a novel member of the UDG superfamily. Our observations suggest that UdgX marks the uracil-dna for its repair by a RecA dependent process. Utility of the tight binding activity of UdgX in detecting uracils in the genomes will be discussed. 44

46 Symposium [S2] : Current Progress in Molecular Genetics and Cell Biology S2-4 Bacterial Epi-metagenomics: Measuring DNA Methylation Patterns in Bacterial Community Level Hoonje Sung, Kyu-Chan Lee, and Woo Jun Sul* Department of Systems Biotechnology, Chung-Ang University DNA methylation in bacteria has played important roles in altering gene expression, regulating cell cycle, and also controlling restriction modification system. Development of Single-Molecule Real-Time (SMRT) DNA sequencing has enhanced the studies of base modification in bacterial genomes. Thus, we further applied SMRT DNA sequencing for measuring and comparing DNA methylation patterns in metagenomes of seawater, soil and cow intestine s microbiomes. We suggest that DNA methylation may be influenced under different environmental conditions. Also, we provided newly built bioinformatic pipeline to search and compare DNA methylation motifs throughout metagenomes. This work will provide new insight of DNA methylation roles in bacterial community level. 45

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48 Symposium [S3] Multiple Interactions between Host and Gut Microbiota Co-organized by Collaborative Genome Program for Fostering New Post-Genome Industry

49 2016 International Meeting of the Microbiological Society of Korea S3-1 Development YOBMT for Improving Metabolic/Immune Disorders Na-Ri Shin, Min-Soo Kim, Hyun Sik Kim, June-Young Lee, Woorim Kang, Dong-Wook Hyun, Pil Soo Kim, Ho-Jun Seong, Eun-Jeong Thak, and Jin-Woo Bae* Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University Human gut microbiota consisting of trillion microorganisms and more than a thousand different bacterial species, plays an important role in metabolism by acting on the regulation of the host s metabolism, and on energy extraction from ingestible foods. Apart from its beneficial functions for the host, gut microbiota can potentially engage in physiological and pathological interactions with the host, particularly in the context of obesity and related metabolic disorders. Metformin has been widely used in the treatment of type 2 diabetes for the last 50 years. The most commonly accepted action mechanism of metformin is the suppression of the transcription of gluconeogenic genes with the activation of AMP-activated protein kinase (AMPK), an enzyme that detects cellular energy levels and regulates fuel availability in the liver. However, there has been no experimental or clinical investigation of the effects of anti-diabetic drugs on the gut microbiota, although gut microbial dysbiosis associated with type 2 diabetes has been described. Questions, therefore, remain about whether metformin, as an anti-diabetic agent, regulates glucose metabolism by modulating gut microbiota. In our previous study, we observed that the metformin treatment significantly improved the glycaemic profile of HFD-fed mice. HFD-fed mice treated with metformin showed a higher abundance of the mucin-degrading bacterium Akkermansia than HFD-fed control mice. In addition, the number of mucin-producing goblet cells was significantly increased by metformin treatment. Oral administration of Akkermansia muciniphila to HFD-fed mice without metformin significantly enhanced glucose tolerance and attenuated adipose tissue inflammation by inducing Foxp3 regulatory T cells (Tregs) in the VAT. Modulation of the gut microbiota (by an increase in the Akkermansia spp. population) may contribute to the antidiabetic effects of metformin, thereby providing a new mechanism for the therapeutic effect of metformin in patients with T2D. This suggests that pharmacological manipulation of the gut microbiota in favour of Akkermansia may be a potential treatment for T2D and we have to isolate the more convincing gut microbes for improving the metabolic disorder. 48

50 Symposium [S3] : Multiple Interactions between Host and Gut Microbiota Owing to the massive collection of exogenous antigens in the intestinal luminal, the immune system must strictly regulate its responses to maintain the symbiotic relation with commensal bacteria. Commensals transmit a signal that induces a tolerogenic response of host immunity. Hence, the host can discriminate between beneficial autochthonous microbes and harmful pathogens, and establish a healthy microbiota. To prevent an inflammatory response to commensal bacteria, gut-residing immune cells, such as mononuclear phagocytes (macrophages and dendritic cells) and CD4+ T cells, are hyporesponsive or display a mutualistic response to microbial stimulationo. At the same time, the mucosal immune system is responsible for clearing pathogens, a process that requires an active proinflammatory signaling cascade. Accordingly, an inappropriate immune response destroys the intestinal homeostasis, triggers dysbiosis, and contributes to local and systemic inflammation and metabolic dysfunction. This state of chronic, progressive intestinal inflammation is clinically diagnosed as inflammatory bowel disease (IBD), which encompasses ulcerative colitis (UC) and Crohn's disease (CD). A precise etiology for IBD is still unavailable, but emerging evidence points to the gut microbiota as the prime suspect in this disease. Thus, in this project, we will develop the YOBMT (Your Own Blended Microbiome Therapeutics) in Korean patients with metabolic/immune disorders. For this goal, we will perform the metagenomic analysis and microbial pipeline development of gut microbiota in mice model with metabolic/immune disorders. Investigations on immune network involving in pathogenesis and defense against chronic inflammatory diseases & development of therapeutic modalities as immune modulators using intestinal commensal microbes, will also be carried out. 49

51 2016 International Meeting of the Microbiological Society of Korea S3-2 Gut Microbiota; the Potential Link to Rheumatic Diseases Seung-Cheol Shim 1 *, Seung-Taek Song 1, Eun-Kyoung Jo 2, Hye-Mi Lee 2, Ji-Young Kim 1, So-Young Lee 1, In-Seol Yoo 1, and Jin-Hyon Kim 1 1 Division of Rheumatology, Daejeon Rheumatoid & Degenerative Arthritis Center, 2 Department of Microbiology and Infection Signaling Network Research Center, Chungnam National University School of Medicine Rheumatic diseases are composed of 120 diseases causing chronic pain in the joints and/or connective tissue. Over time, the search led to correlative studies of specific bacteria and viruses in the pathogenesis of these disorders, most notably rheumatoid arthritis (RA), psoriasis, inflammatory bowel disease (IBD), and spondyloarthritides (SpA). SpA is a family of immune-mediated inflammatory disorders that includes ankylosing spondylitis (AS), psoriatic arthritis (PsA), and acute anterior uveitis. There is considerable clinical overlap between SpA and IBD exhibiting shared genetic predisposition and pathogenic mechanisms. IBD has been long associated with alterations in the gut microbiome, which may be primary or secondary factors in disease pathogenesis. Rats overexpressing HLA-B27 spontaneously develop an inflammatory disease exhibiting arthritis and colitis, thus, mimicking human SpA. HLA-B27 alters the intestinal microbiome, which might be the basis for disease predisposition associated with this allele. This concept is supported by theories of a disrupted gut environment in SpA, with altered intestinal permeability perhaps leading to a dysregulated immune response and/or altered dendritic-cell function. Here, we review recent developments from studies of the gut microbiome in patients with AS and SpA as well as insights obtained from the animal models of SpA, and introduce our study regarding to the effect of NSAIDs on the microbiome in a SpA animal model. 50

52 Symposium [S3] : Multiple Interactions between Host and Gut Microbiota S3-3 An Updated Evaluation of Next Generation Sequencing and Bioinformatics for Microbiome Analysis Jongsik Chun School of Biological Sciences, Seoul National University & ChunLab, Inc. Microbiome has become a key in understanding our health and diseases. Analysis of microbiome largely depends on the high-throughput sequencing called Next Generation Sequencing (NGS). Because NGS instruments are continuously improved and new ones are introduced, handling sequence data generated from new instruments is becoming important, especially for the retrospective data compatibility. Since Roche 454, which has been widely used in microbial community analysis, is expected to retire, it is necessary to evaluate new NGS platforms such as Illumina MiSeq and Pacific Biosciences (PacBio) long read sequencers. In this talk, I will present some of data generated from MiSeq and PacBio and discuss about bioinformatics strategies. 51

53 2016 International Meeting of the Microbiological Society of Korea S3-4 Comparative Swine Fecal Microbiota Analysis Across Breeds, Regions, Growth Stages, and Antibiotics Feed Additives Tatsuya Unno*, Jungman Kim, Nguyen G. Son, and Robin B. Guevarra Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University Pork is one of the major markets in Jeju. While feed improvements and application of probiotics have shown significant development in promoting swine growth and health, understanding theses effects require basic understanding of swine gut microbiota. We have collected swine fecal samples for three years and conducted gut microbiota comparison across breeds, regions, growth stages, and feeding conditions (i.e, antibiotics feed additives). Our results showed that black and white pigs showed slightly different gut microbiota in Firmicutes/ Bacteroidetes ratio and species diversities. In contrast, there was no significant difference between gut microbiota of Yorkshire and Landrace. Regional differences were seen among swine in Gwagnju, Haenam, and Jeju. During weaning, piglets have prevalent Prevotellaceae and comprised with various species, whereas finisher swine had relative less variations across individuals. Tetracycline-based antibiotics feed additives did not promote weight gain, but found effective in suppressing Spirocheates in early growth stage. Likewise, tylosin-based antibiotics feed additives did not promote weight gain, but seemed to accelerated maturation of gut microbiota. Although these studies were done with relatively small sample size, the study suggest that swine gut microbiota are very condition-sensitive. 52

54 Symposium [S3] : Multiple Interactions between Host and Gut Microbiota S3-5 Changes in the Swine Fecal Microbiota by the Administration of Probiotics and Prebiotics Dae-Kyung Kang*, Jong Pyo Chae, and Edward Alain B. Pajarillo Department of Animal Resources Science, Dankook University Demand for the development of non-antibiotic growth promoters (AGP) in animal production surged in recent years. However, elucidating the specific mechanisms and action of prebiotics, probiotics, and synbiotics as non-agp in animals is still in progress. This work investigated and compared fecal microbiotas of weaned piglets under the administration of a basal diet (CON) and with prebiotic lactulose (LAC), probiotic Enterococcus faecium NCIMB (PRO), or their synbiotic combination (SYN). Although prebiotics and/or probiotics in the diet significantly increased α-diversity compared with CON values, no differences were detected in richness and diversity values among the treatment groups (LAC, PRO, and SYN). At phylum level, the Firmicutes to Bacteroidetes ratio increased in all treatment groups in comparison to the CON group, and the lowest abundance of Proteobacteria was found in LAC group. At family level, Enterobacteriaceae decreased in all treatment groups, especially more than 10-fold reduction in LAC group compared with CON group. At genus level, the highest abundance of Oscillibacter was detected in PRO group, the highest Clostridium in LAC group, and the highest Lactobacillus in SYN group; the abundance of Escherichia was lowest in LAC group. Clustering in the DAPC plots illustrated distinct separation of the feeding groups (CON, LAC, PRO, and SYN) from one another, showing that microbial communities had different compositions according to different feed additives. Effects of LAC and PRO treatments on the faecal microbiota suggest independent mechanisms; nonetheless, the impact of synbiotics might also be distinct from that when each are administered singly as lactulose or E. faecium. 53

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56 Symposium [S4] Microbes Meet Radiation: Gene to Industry Sponsored by Korea Atomic Energy Research Institute

57 2016 International Meeting of the Microbiological Society of Korea S4-1 Analysis of Deinococcus deserti : Species-specific Characteristics and Improved Characterization of Radioresistance in the Deinococcaceae Laurence Blanchard, David Pignol, and Arjan de Groot* Laboratory of Cellular Bioenergetics, Biosciences and Biotechnology Institute of Aix-Marseille, French Alternative Energies and Atomic Energy Commission, France Bacteria belonging to the genus Deinococcus are famous for their extreme tolerance to radiation, desiccation and other oxidative stress- and DNA damage-generating conditions. This tolerance is related to their capacity to repair massive DNA damage and might result from a combination of different molecular mechanisms and physiological determinants, including protection of proteins against oxidative damage. More than 50 Deinococcus species have been described, of which Deinococcus radiodurans has been studied most extensively. To better characterize radiation resistance in the Deinococcaceae, we are studying Deinococcus deserti, a species isolated from the Sahara in The approaches we use include (comparative) genomics, transcriptomics, proteomics, genetics, biochemistry and structural biology. D. deserti has many genes and characteristics in common with D. radiodurans and other Deinococcus species, but several interesting differences have also been identified. One example concerns RecA, a crucial DNA repair protein. Remarkably, D. deserti contains three different reca genes that code for two functionally different RecA proteins. Both RecA allow repair of massive DNA damage, but only one of these RecA facilitates radiation-induced expression of translesion DNA polymerases involved in error-prone lesion bypass. The transcriptome of D. deserti was analysed using RNA sequencing. Strikingly, this revealed an exceptionally high proportion (60%) of mrnas that are leaderless (i.e., lacking a 5ʹ-untranslated region and Shine-Dalgarno ribosome-binding site). Proteomics showed that leaderless mrnas are efficiently translated in D. deserti. The essential nucleoid protein HU is an example of a protein highly expressed from leaderless mrna. Interestingly, many novel transcripts were identified and predicted to correspond to leaderless mrnas encoding small peptides, providing an explanation for the generation of a cellular pool of small peptides important for protection of proteins against oxidation. The transcriptome and proteome data were also important for an improved genome annotation, including correction of the 56

58 Symposium [S4] : Microbes Meet Radiation: Gene to Industry translation initiation codon position of many genes in D. deserti and also in other Deinococcus species. The irre gene was first identified in D. radiodurans as a novel gene required for radiation resistance and for the radiation-induced expression of several DNA repair and other genes. The mechanism by which the IrrE protein is involved in gene induction remained unknown for many years. We solved the crystal structure of D. deserti IrrE, and recently demonstrated a novel radiation response mechanism by showing that IrrE is a metalloprotease that cleaves and inactivates a transcriptional repressor protein called DdrO after exposure of the cells to radiation. The analysis of different genome sequences strongly suggests that the IrrE/DdrO-regulated stress response mechanism is common in all members of the Deinococcaceae. 57

59 2016 International Meeting of the Microbiological Society of Korea S4-2 Enhanced Stress Tolerance of Escherichia coli Expressing Deinococcal Genes Sangyong Lim Research Division for Biotechnology, Korea Atomic Energy Research Institute Cellular robustness of industrial microbes is an important trait because the microbial strains are exposed to a multitude of different stresses during industrial processes, such as fermentation. Thus, engineering robustness in an organism has become a significant topic of research in order to push the strains towards maximizing yield. Deinococcus radiodurans (D. radiodurans) is one of the most highly stress-resistant species reported. It can withstand extremely high doses of ionizing radiation, long periods of desiccation, UV radiation and oxidizing agents. Stress responsive genes of D. radiodurans have been used to enhance stress tolerance of Escherichia coli (E. coli). In this study, we introduced the deinococcal response regulator DR1558 and the cold shock protein PprM into E. coli and found that the tolerance to hydrogen peroxide (H 2 O 2 ) was significantly increased in the recombinant strains. DR1558 bound to the rpos promoter, thereby increasing the RpoS expression. E. coli cells expressing DR1558 were able to tolerate to low ph, high temperature, and high NaCl concentrations in addition to H 2 O 2, and the multi-stress tolerance phenotype disappeared in the absence of rpos. Overexpression of PprM in E. coli led to elevated expression of some OxyR-dependent genes, such as mnth (manganese transporter) and hemh (ferrochelatase), and the ycgz-ymgabc operon, which encode proteins involved in biofilm formation and acid resistance. We confirmed that co-expression of the ycgz-ymgabc operon conferred H 2 O 2 tolerance to E. coli. In the present study, we demonstrated a strategy of employing stress responsive genes from radiation resistant bacteria for strain improvement. 58

60 Symposium [S4] : Microbes Meet Radiation: Gene to Industry S4-3 Single DNA Molecules Analysis for UV Induced Damage in E. coli Kyubong Jo Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University Radiation is a powerful stress factor to cause acute and chronic DNA damage. Exposure to UV radiation may result in most detrimental DNA damage by direct energy deposition or reactive oxygen species. Although the radiation stress is clinically or therapeutically important for health care, analytical approaches are limited for characterizing radiation induced DNA damaged lesions. To overcome the limitations, we developed single-molecule visualization for DNA damage analysis caused by radiation induced metabolism using fluorescent labels. Furthermore, we quantitatively analyzed DNA damage in a wild type strain and highly radiation tolerant E. coli strains generated by gamma ray induced evolution. Our approach demonstrated high sensitivity that we could count the number of radiation induced DNA damage lesions in wild type strain and radiation tolerant strain. Moreover, we also observed nucleotide sequence changes in reca, dnab, and yfjk genes by combining the single molecule optical mapping systems and next generation sequencing. 59

61 2016 International Meeting of the Microbiological Society of Korea S4-4 Development and Evaluation of Irradiated Bacterial Vaccine Ho Seong Seo Department of Biotechnology, Korea Atomic Energy Research Institute Many vaccines used today rely on technologies developed over 100 years ago, and involve some forms of attenuation (i.e., the use of an alternative or mutant strain of pathogenic organism with reduced virulence that maintains its immunogenicity) or inactivation, where chemical or physical methods are used to kill virulent pathogenic strains. Although these vaccines have been extremely successful in protecting against animal and human infectious diseases, there is a large demand for the development of fast, safe, and effective vaccine manufacturing strategies. Radiation sterilization has been used to develop a variety of vaccine types, because it can eradicate chemical contaminants and penetrate pathogens to destroy nucleic acids without damaging the pathogen surface antigens. In addition, radiation mutation technology which has been used for breeding plants would be a great tool to create fast and safe attenuated vaccine strains. Nevertheless, irradiated vaccines have not widely been used at an industrial level because of difficulties obtaining the necessary equipment. Recent successful clinical trials of irradiated vaccines against pathogens and tumors have led to a reevaluation of radiation technology as an alternative method to produce vaccines. Here, I will review the challenges associated with creating irradiated vaccines and introduce how radiation technology are applied to develop bacterial vaccines in our institute. 60

62 Symposium [S5] Molecular Microbiology of Pseudomonas aeruginosa

63 2016 International Meeting of the Microbiological Society of Korea S5-1 Regulation of Anthranilate Metabolism and Its Effect on Biofilm Formation in Pseudomonas aeruginosa Soo-Kyung Kim, Xi-Hui Li, and Joon-Hee Lee* Department of Pharmacy, College of Pharmacy, Pusan National University Anthranilate (AA) is an important intermediate in the syntheses and degradation of tryptophan in many organisms including an opportunistic human pathogen, Pseudomonas aeruginosa. AA is also a precursor for the synthesis of Pseudomonas quinolone signal (PQS; 2-heptyl-3-hydroxyl-4-quinolone) in P. aeruginosa. In that tryptophan is an essential amino acid and PQS is a quorum sensing (QS) signal, AA is a key intermediate at the metabolic branch point in P. aeruginosa. Regulation of the AA synthesis and degradation is finely tuned by QS systems. RhlR and LasR regulate the AA metabolism in a mutually antagonistic, and growth phase-dependent manner. QscR is also involved in this regulation by repressing both LasR and RhlR functions in a growth phase-dependent manner. This timely regulation by the antagonistic interplay of the QS regulators is mediated by two intermediate regulators, AntR and PqsR, and their cofactors, AA and PQS. AntR, a LysR-type regulator activates the transcription of antabc operon encoding AA dioxygenase complex that functions to degrade AA. In P. aeruginosa, antabc and antr (encoding AntR) genes are divergently located. In the presence of AA, AntR binds to two AntR-responsive elements (AREs) at the intergenic region between anta and antr, and bidirectionally activates both antabc and antr transcriptions. Both AREs are important in this bidirectional activation, but AntR has different binding affinity to each ARE. As a result, the strength of transcriptional activation becomes dramatically asymmetric depending on the direction. In nature, there are many AA-producing bacteria as a tryptophan degradation product. So, microorganisms that exist in tryptophan-rich environments necessarily encounter exogenous AA as well as endogenously produced AA. Interestingly, AA deteriorates the biofilm structure of P. aeruginosa. AA exerts this anti-biofilm effect by reducing the level of intracellular c-di-gmp and modulating the expression of Psl, Pel, and alginate, major extracellular polymeric substances (EPSs) of P. aeruginosa. AA also has a significant deteriorating effect on biofilms of other bacteria, such as Vibrio vulnificus, Bacillus subtilis, and Staphylococcus aureus. Since AA significantly enhanced swimming and swarming motility of P. aeruginosa, V. vulnificus, and B. subtilis, we suggest that the enhanced motility causes the biofilm-deteriorating effect of AA. These results suggest that AA may be a promising candidate for the development of anti-biofilm agent. 62

64 Symposium [S5] : Molecular Microbiology of Pseudomonas aeruginosa S5-2 The Role of Pseudomonas aeruginosa DesB on Virulence Traits and Staphylococcus aureus Growth Inhibition in the Same Ecological Niche Kyoung-Hee Choi 1 *, Sejeong Kim 2, Jimyeong Ha 2, and Yohan Yoon 2 1 Department of Oral Microbiology, College of Dentistry, Wonkwang University 2 Department of Food and Nutrition, Sookmyung Women s University Most microbes exist primarily in mixed microbial communities, which affect interspecies interaction and alter clinical outcomes. Pseudomonas aeruginosa usually coexists with other pathogens, such as Staphylococcus aureus. P. aeruginosa impedes the growth of S. aureus by secreting toxic substances such as alkyl-hydroxyquinoline N-oxides, hydrogen cyanide, and pyocyanin. Virulence factor production by P. aeruginosa is extremely important for growth and pathogenesis in polymicrobial environments. A mutant harboring a transposon insertion in the desb gene, encoding a desaturase, displayed significantly reduced the production of various exoproducts, including elastase, protease, pyocyanin, and rhamnolipids, as well as decreased motility, proving that DesB plays an important role in P. aeruginosa virulence. In addition, we found that the desb mutant exhibited reduced S. aureus growth inhibition compared to the wild-type (WT) strain. The transcriptional profiles of the WT and desb mutant revealed that the expression of MvfR-controlled pqsa-e and phnab operons was significantly decreased, but the mexef-oprn operon was highly expressed. The results indicate that increase in MexEF-OprN efflux pump expression causes reduced intracellular levels of 4-hydroxy-2-heptylquinoline (HHQ), a ligand of MvfR, in desb mutant, leading to the decrease of MvfR binding to pqsa-e promoter and the reduction of 4-hydroxy-2-alkylquinolines (HAQs) synthesis. In conclusion, these results suggest that DesB contributes to virulence, and promotes the inhibition of S. aureus growth by regulating HAQ synthesis. 63

65 2016 International Meeting of the Microbiological Society of Korea S5-3 Making Pseudomonas aeruginosa Sensitive to Lysozyme Sang Sun Yoon* and Kang-Mu Lee Department of Microbiology and Immunology, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine Pseudomonas aeruginosa, a Gram-negative bacterium of clinical importance, can establish airway infections in patients suffering from pneumonia, cystic fibrosis (CF), bronchiectasis and COPD. Human airway is lined with mucus layer that contains a large amount of lysozyme, an enzyme that hydrolyzes bacterial cell walls, and thus can suppress bacterial growth on the airway surface. Of note, elevated lysozyme activity was observed in the bronchoalveolar lavage fluid (BALF) derived from CF patients, suggesting that the degree of P. aeruginosa infection may not correlate with the level of lysozyme in the airway. Consistent with this notion, P. aeruginosa has been known to be resistant to lysozyme treatment. In this work, we performed a forward genetic screening using a random transposon (Tn) insertion mutant library of PAO1, a prototype strain of P. aeruginosa and identified three mutants that became sensitive to lysozyme treatment. PAO1 mutants defective in PA0420 (bioa), PA3800 (bamb), or PA5174 showed significant growth defects in the presence of lysozyme (1 mg/ml). Each of these mutants exhibited reduced virulence in an acute mouse airway infection model. Molecular mechanisms behind these sensitivities and potential implications of these findings with regards to the P. aeruginosa infection control will be presented. 64

66 Symposium [S5] : Molecular Microbiology of Pseudomonas aeruginosa S5-4 Secreted Factors of Pseudomonas aeruginosa for the Modulation of Innate Immune Responses Un-Hwan Ha Department of Biotechnology and Bioinformatics, Korea University The clinical impact of polymicrobial diseases, caused by combinations of pathogens, has received much attention from the medical community. Pseudomonas aeruginosa is a bacterial pathogen that is prone to infect the respiratory tract of immunocompromised patients along with other microbial invaders. P. aeruginosa possesses a number of virulence factors and secretory systems, which play a critical role in causing acute and chronic infections. The potential effects of these factors on the modulation of host inflammatory responses against competitive bacteria, such as Staphylococcus aureus, are unknown. Here, we report that human bradykinin receptors as important host defense responses against invading microbes are up-regulated by components secreted from P. aeruginosa, and the secretion of the components is not controlled by either T3SS or quorum sensing. In addition to this, P. aeruginosa infection induces the expression of TLR2, which plays a dominant role in sensing PAMPs expressed by Gram-positive bacteria. Upregulation of TLR2 influences the magnitude of proinflammatory responses to the secondary S. aureus infection. Moreover, P. aeruginosa Ndk, with the aid of flagellin, induces the expression of interleukin-1. Cytokine induction appears to be dependent on the kinase activity of Ndk, and the Ndk activates the Akt signaling pathway, which acts upstream of NF-κB as well as caspase-1. Taken together, the results of this study demonstrate that P. aeruginosa possesses diverse virulence factors that are released and subsequently modulate innate immune responses, and they may have impacts on against a secondary microbial infection. 65

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68 Symposium [S6] Omics Approaches in Systems Microbiology

69 2016 International Meeting of the Microbiological Society of Korea S6-1 In Pursuit of High-quality Reference Genomes: Causes of Failed Illumina Assemblies of Microbial Genomes and Their Improvements Haeyoung Jeong 1,2 *, Jung Hoon Sohn 3, Jae-Goo Pan 1, and Seung-Hwan Park 1,2 * 1 Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) 2 Biosystems and Bioengineering Program, University of Science and Technology (UST) 3 Cell Factory Research Center, KRIBB Advances in next-generation sequencing technologies and concomitant cost reduction have brought about genome sequencing in everyday use for any laboratories ( democratization of sequencing ). As successful assembly of complete microbial genomes using the PacBio platform and nonhybrid hierarchical assembly process is in the spotlight, there is a slight chance of re-evaluating failed microbial genome assembles (high contig numbers, large total contig size and/or the presence of low-coverage contigs) among massively produced Illumina sequencing reads. Using k-mer abundance analysis, we first tried to detect diagnostic signatures from contamination-free Illumina reads that previously led to poor assemblies. Some sequencing reads with an extraordinary peak at low-frequency k-mer range, which could not be assembled normally despite conventional pretreatments including adapter sequence removal and quality trimming, were successfully assembled after filtration of reads with low abundance k-mer or subsampling of reads. Second, simulated reads from pairs of bacterial chromosome sequences (difference species or different strains) were combined and assembled to investigate the effect of sequence differences and the ratios of mixed reads. The worst assembly was obtained when simulated reads from different strains (~1% difference) were mixed at 1:1. This observation led us to the idea that poor assemblies of some yeasts genomes might be due to heterozygous diploid status that were yet unknown for the sequencing subjects. Using Illumina reads from diverse source encompassing laboratory yeast strains to natural isolates (genera Kluyveromyces, Issatchenkia, Candida and Cryptococcus), we are testing this hypothesis by combination of different values of k-mer and bubble size during de novo assembly and by measurement of SNP frequencies after re-mapping of reads on the assemblies. 68

70 Symposium [S6] : Omics Approaches in Systems Microbiology S6-2 Understanding Virulence Regulation of Salmonella Typhimurium Using Proteomic Profiling of Outer Membrane Vesicles Hyunjin Yoon 1,2 *, Jaewoo Bai 3, Seul I Kim 1, and Seo Yeon Hwang 1 1 Department of Molecular Science and Technology, Ajou University 2 Department of Applied Chemistry and Biological Engineering, Ajou University 3 Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Seoul National University Salmonella enterica serovar Typhimurium is a primary cause of enteric diseases and has acquired a variety of virulence factors during its evolution into a pathogen. Secreted virulence factors interact with commensal flora and host cells and enable Salmonella to survive and thrive in hostile environments. Outer membrane vesicles (OMVs) released from many Gram-negative bacteria function as a delivery vehicle for complex molecules, including virulence factors. In order to understand the roles of OMV in virulence regulation of Salmonella, a proteomic analysis was conducted on OMVs harvested under two different conditions mimicking the infection environments. Comparative proteomic profiling between two conditions identified 14 proteins that were associated with the OMV fraction isolated only under the acidic minimal medium conditions, which reproduced the nutrient-deficient intracellular milieu. The absence of these 14 proteins each influenced Salmonella survival inside host cells (either increased or decreased), proposing these OMV-associated proteins as new virulence factors in Salmonella. Another valuable finding is that OMV was able to deliver some virulence effectors that have been known to be secreted via Salmonella pathogenicity island (SPI) -1 or -2 type three secretion systems (T3SSs). OMVs possessing SPI-1 effectors on the surface increased the amount of F-actin contents in the host cell membrane, when added to the culture of epithelial cells, whereas OMVs lacking SPI-1 effectors did not influence the level of F-actin in host cells. This result suggests a role of OMV as an alternative delivery system to T3SSs. Proteomic profiling provides a deeper insight into how Salmonella exploits OMV to interact with the environments. 69

71 2016 International Meeting of the Microbiological Society of Korea S6-3 Computational Genomics Approach for Elucidation of Core Genes in Agarolytic Pathway In-Geol Choi*, Byeong Hyeok Park, Saeyoung Lee, Duleepa Pathiraja, and Kyoung Heon Kim Department of Biotechnology, Graduate School, Korea University Agar is a major cell-wall constituent found in marine red algae. While agar has been utilized as a gel medium for pure culture technique, its resistance to microbial degradation hampered the utilization of red algal biomass. The metabolic fate of agar is not fully understood. Agar is a hetero-polysaccharide composed of two monomeric units: D-galactose (D-GAL) and L-anhydrogalactose (L-AHG). Like other polymer degradation, the metabolic pathway of agar has a typical pattern dividing into distinct steps. So as to use agar as raw materials, we collected representative genes involved in the agarolytic pathway. Based on a computational genomics approach, we predicted the core gene set for microbial agarolytic pathway. Using known functional agarases and hydrolases as a probe, we surveyed 2,759 microbial genomes in the public database and selected 12 potential agarolytic genomes. RNAseq analysis of three agarolytic microorganisms, Saccharophagus degradans, Marinimicrobium agarolyticum and Vibrio sp. EJY3 corroborated the core gene set. The predicted core genes provide a minmal gene set for design and construction of a synthetic agarolytic system. 70

72 Symposium [S6] : Omics Approaches in Systems Microbiology S6-4 Transcriptome and Network Analysis of Butanol Stress in Escherichia coli Haeyoung Jeong 1 and Sung Ho Yoon 2,3 * 1 Super-Bacetria Research Center, Korea Research Institute of Bioscience and Biotechnology 2 Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology 3 Department of Bioscience and Biotechnology, Konkuk University Butanol is a promising alternative to ethanol and is desirable for transportation fuels and additives of gasoline and diesel fuels. However, microbial production of butanol is challenging primarily due to butanol toxicity and low titer of butanol production. Here, we analyzed and compared transcriptome of wild-type E. coli and its butanol-tolerant mutant to understand global cellular physiology and metabolism responsible for the butanol tolerance. Gene association network of E. coli was interrogated to understand the roles of mutated genes in the butanol-tolerant mutant. The mutated genes showed correlated relationship between the gene expression change and degree of network connection. The analyses identified potential gene candidates that can be engineered to increase butanol tolerance in E. coli. 71

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74 Symposium [S7] Evolution and Ecology of Lichens

75 2016 International Meeting of the Microbiological Society of Korea S7-1 Diversity of Symbiotic Microalgae in Lichens Chae Haeng Park, Kyuin Hwang, and Soon Gyu Hong* Divison of Polar Life Sciences, Korea Polar Research Institute Lichens are symbiotic organisms that are mainly composed of lichenized fungi (mycobiont) and photosynthetic microalgae and/or cyanobacteria (photobiont). It has long been regarded that one fungal species make symbiotic relationship with one microalgal species in a thallus. However, the specific relationship between the mycobiont and the photobiont has been challenged by recent studies. One species of mycobiont can make symbiotic partnerships with various photobiont species when they grow at geographically distant locations. Several different algal genotypes can be present in a single lichen thallus. In addition, the results of the algal community composition in lichens from King George Island, Antarctica indicated that each lichen thallus contained diverse algal species and the composition of algal community was mostly related to the mycobiont species. In this study, the genetic diversity and composition of symbiotic microalgae populations in the widespread geographical distribution of the seven lichen genera, Cetraria, Cladonia, Ocheloechia, Psoroma, Stereocaulon, Usnea, and Umbilicaria, were investigated based on eukaryotic LSU rrna gene. To understand the effect of geography and climate on microalgal diversity, samples were collected from bi-polar and sub-polar regions. The results revealed that each lichen thallus contained diverse microalgal OTUs as the previous studies. However, each mycobiont genus showed preference on specific lineage of microalgal species as a major photobiont partner, which is composed of phylogenetically related OTUs. Although some microalgal OTUs were detected from several regions including Southern and Northern hemisphere regardless of climate, most microalgal OTUs were recovered only from specific geographical region and climatic zone. Considering these results, we conclude that the composition of microalgal community in lichens are affected by mycobiont speceis, geography, and climate. 74

76 Symposium [S7] : Evolution and Ecology of Lichens S7-2 Comparative Genome Analysis of Lichen-forming Fungi and Partner Algae Sook-Young Park 1, Hyunjung Song 2, Jung A Kim 1, Jaeyoung Choi 2, Yong-Hwan Lee 2, and Jae-Seoun Hur 1 * 1 Korean Lichen Research Institute, Sunchon National University 2 Department of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University Lichens are symbiotic organisms, composed of a fungal partner (the mycobiont) and at least one eukaryotic algal or cyanobacterial species (the photobiont). As demonstrated by the world-wide distribution of lichens in various kinds of habitats from the tropics to the Polar regions, lichen symbiosis seems to be a highly successful adaptation to a diverse range of environmental conditions. To get insight in the genetic features linked to the symbiosis in both fungi and algae, whole-genome sequences of five lichen-forming fungal isolates and two algal isolates were determined. For the five sequenced fungal genomes, average size and the number of predicted genes were Mb and 97,468, respectively, and two sequenced algal genomes, average size and the number of predicted genes were Mb and 8,995, respectively. We explored genomic features including genes encoding small secreted proteins (SSPs), polyketide synthesis genes, carbohydrate active enzyme-related genes, cytochrome P450 genes, and transcription factor genes. In addition, genome and proteome conservation analysis revealed that the lichen-forming fungal genomes share the majority of genetic materials in common, when compared in a pairwise manner. To gain a better understanding of the molecular determinants of symbiosis, we performed RNA-seq and analyzed gene expression during resynthesis between fungus and alga. We reveal that a number of genes encoding SSPs are involved in symbiosis during the resynthesis. The availability of both fungal and algal genomes will provide an opportunity to decipher an understanding of the processes by which symbionts interact between both organisms. Our study will enhance our understanding of the adaptive evolution of the lichen-forming fungi with the algae to their ecological niches. 75

77 2016 International Meeting of the Microbiological Society of Korea S7-3 Pannariaceae Lichens: Model Organisms for Global Evolutionary Patterns Arve Elvebakk 1 *, Chae Haeng Park 2, and Soon Gyu Hong 2 1 University of Tromsø, the Arctic University of Norway 2 KOPRI Lichens of the family Pannariaceae are widespread throughout the world, from Antarctica, throughout temperate and tropical areas and further into the Arctic. The family is evolutionary old, but its age is uncertain, as its group has been dated as either 270 or 180 Ma old. The family has four major branches recognized by all recent phylogenetic studies. One is tropical, the remaining three are cosmopolitan. However, austral Gondwanaland distributions dominate in two of them and Northern Hemisphere distributions in the last one. These contrasting distributions all indicate a long evolutionary history. We have very recently expanded the tropical branch significantly by describing the new tropical genus Gibbosporina with 12 new species, tentatively dated as 75 Ma old. What came as an additional surprise, was that Xanthosporoma, another genus described as new to science by us, in 2010, came out as a much older, but inaccurately defined link between the tropical branch and the two mainly austral branches. More samples and more genes are now being analyzed to further study this topic. The genus Xanthopsoroma is very small with only two known species, but is very distinct in several characters, and related to Psorophorus, another new genus from our 2010 paper. Pannariaceae representatives were certainly present in the old Gondwanaland forests when the southern continents were much closer to each other than today, and when southern beech forest dominated in Antarctica. After the opening of the Drake Strait and the cooling of Antarctica, representatives of some genera adapted to a cold, tree-less, terricolous habitat, in particular species of Psoroma. They have some representatives on tree trunks in austral forests, however, no less than 7 species are accepted today from Antarctica. We will describe a new one ( P. antarcticum in prep. ), revise/ revive three more from Antarctica, and describe quite a number of additional new species from southernmost South America, New Zealand, and northernmost Europe, and single ones from South Africa and Alaska. A particular interesting case is one Psoroma which migrated from Antarctic/Subantarctic areas into the Northern Hemisphere, possibly in the early Quaternary. Here it developed into the species P. hypnorum as shown by its very wide genetic diversity analyzed from Norwegian material. Much more recently it had 76

78 Symposium [S7] : Evolution and Ecology of Lichens another rare, casual, long-distance dispersal into Antarctica/neighbouring areas, where it now has a much more narrow genetic diversity, mostly also different, probably meriting status as a separate subspecies. Pannaria is another key genus. We are now developing a phylogeny, still partly hypothetical, of 9 expected major clades, with six different world distribution patterns. Most austral clades have three symbiotic partners, are still very insufficiently known with regard to chemistry, and details in spore morphology and sexual organs. Eight new species have been described by our group so far, the number will probably be tripled. One of the clades has as a polar distribution, with one bipolar species and some subantarctic species partly revised recently. A particular case is a 10 th clade, provisionally kept within Pannaria, but differing from the remaining ones by forming a black hyphal mat carrying rather small discrete lichen scales/squamules. We believe that this is a heterogeneous group, representing at least three undescribed genera, some small with an apparently long and isolated evolutionary history in southernmost South America and New Zealand, respectively. However, their classification based on two genes has not been stable so far. Our present project aims to improve the classification considerably, involving five genes, many more sequences, and to elucidate the evolutionary history of the Pannariaceae lichen family on a global scale. Clade formations will probably reflect the old separation of Gondwanaland from Laurasia, the split-up of Gondwanaland, Tertiary cooling which formed a cold Antarctica, and later dramatic Quaternary glaciations. It has also shown local endemism developing in isolated austral islands, contrasting wide distributions in isolated islands within tropical cyclone belts. 77

79 2016 International Meeting of the Microbiological Society of Korea S7-4 Recent Progress of Korean Lichen Biodiversity Survey: Korea National Arboretum Project Jae-Seoun Hur Korean Lichen Research Institute, Sunchon National University In common with other areas of East Asian regions, the lichen flora of South Korea is little known. Lichenological studies in this area dates back to 1909 when Hue for the first time reported the occurrence of L. oreina Ach. from this place. From then until 1945, Korean lichens were studied mostly by Japanese lichenologists. But it was in 1960s, that Korean lichenologists started studying lichens by themselves and the first publication of Korean lichenologists came in the year However, Korean lichenology came into limelight in the year 1990, when Park made her first international publication on macro-lichens of South Korea. After Park s contribution, several other workers (K. H. Moon, J. S. Hur) developed a keen interest in Korean lichens and started working on them. The first checklist of Korean lichens came in the year 2005, mentioning the occurrence of 113 genera and 510 species. This number pretends to be too few for Korea, in comparison to some European countries, like Greece etc., which have much smaller geographical area but enormous lichen diversity. This point is further clarified by National wide survey supported by Korean National Arboretum during the last 10 years. Recent progress of Korean lichen biodiversity and database preparation is discussed in this presentation. KEYWORDS: New species, New records, Lichenized fungi, South Korea, Taxonomy 78

80 Symposium [S8] Probiotics and Gut Homeostasis Sponsored by Korea Yakult

81 2016 International Meeting of the Microbiological Society of Korea S8-1 Characterization of Weissella cibaria Plasmid and Construction of Weissella Minimal Shuttle/Expression Vector Ju-Hoon Lee Department of Food Science and Biotechnology, Kyung Hee University A 2.1-kb plasmid was previously isolated from Weissella cibaria KLC140 in kimchi and cloned into puc19 along with the slpa and gfp genes, resulting in an 8.6-kb pkwcslgfp construct for use as a novel surface display vector. To reduce the size of the vector, the minimal replicon of pkw2124 was determined. The pkw2124 plasmid contains a putative origin of replication (ori), a potential ribosomal binding site (RBS), and the repa gene encoding a plasmid replication protein. To conduct the minimal replicon experiment, four different PCR products (MR1, ori + RBS + repa; MR2, RBS + repa; MR2, repa; MR3, fragment of repa) were obtained and cloned into puc19 (pkucm1, pkucm2, pkucm2, and pkucm3, respectively) containing the chloramphenicol acetyltransferase (CAT) gene. These constructed vectors were electroporated into W. confusa ATCC with different transformation efficiencies of CFU/μg, CFU/μg, and no transformation, respectively, suggesting that the putative ori, RBS, and repa gene are essential for optimum plasmid replication. Subsequent segregational plasmid stability testing of pkucm1 and pkucm2 showed that the vector pkucm1 is highly stable up to 100 generations but pkucm2 was completely lost after 60 generations, suggesting that the putative ori may be important for plasmid stability in the host strain. In addition, a host range test of pkucm1 revealed that it has a broad host range spectrum including Weissella, Lactococcus, Leuconostoc, and even Lactobacillus. To verify the application of pkucm1, the β-galactosidase gene and its promoter region from W. cibaria KSD1 were cloned in the vector, resulting in pkugal. Expression of the β-galactosidase gene was confirmed using blue-white screening after IPTG induction. The small and stable pkugal vector will be useful for gene transfer, expression, and manipulation in the Weissella genome and in other lactic acid bacteria. 80

82 Symposium [S8] : Probiotics and Gut Homeostasis S8-2 Approaches to the Microbial Ecology of Food Systems Gisèle LaPointe Department of Food Science, University of Guelph, Guelph, Ontario, Canada Culture-independent analytical methods have provided the means to study the diversity as well as the transcriptional activity of microbial communities in food and gut systems. The rapid accumulation of genome sequences has also greatly contributed to the expansion of molecular technologies. Now in the postgenomic era, multiphasic approaches combining analysis of DNA, RNA, proteins and metabolites are being applied to microbes in many systems. Cheddar cheese defined starter cultures are generally composed of selected strains of Lactococcus lactis, and their interactions with Lactobacilli of the non-starter microbiota contribute to the development of typical Cheddar flavour. While genotyping reveals basic genetic diversity and strain signatures, differential gene expression profiles give us indicators of transcriptional activity in the cheese matrix and provide biomarkers that can be used to track the effect of process parameters on microbial activity and performance during cheese ripening or during digestion. Studies using PMA-qPCR differentiate viable from non-viable probiotics in cheese and during in vitro digestion, revealing the effect of using multiple strains on their survival. The antioxidant capacity of fermented milk containing Bifidobacterium longum strains actually increases over TIM1 in vitro digestion. Understanding microbial diversity and their interactions will greatly aid our ability to predict their performance in order to design strain mixes to improve dairy product quality and their health impact. 81

83 2016 International Meeting of the Microbiological Society of Korea S8-3 Rationally Selected Probiotics for Hyper-immune Disorders Ravi Verma 1, Changhon Lee 1,2 *, Eunji Jeun 1,2, and Sin-Hyeog Im 1,2 * 1 Academy of Immunology and Microbiology (AIM), Institute for Basic Science (IBS) 2 Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH) Probiotics are nonpathogenic live microorganism that can provide a diverse health benefits on the host. Recently, many reports suggest that certain probiotic strains or mixture of them could exert potent immunomodulatory activity in diverse disorders. However, efficacy of probiotics is quite different depending on the type of strains and the amounts of doses. We have developed a screening system to selectively identify probiotic strains that could generate CD4+Foxp3+ regulatory T cells (itregs). Among the strains, we found that Bifidobacterium bifidum IRT, and Lactobacillus reuteri IRT showed the higherst IL-10 high Il-12 low inducing capability. In this study, we tested whether monocolonization of B. bifidum IRT, and L. reuteri IRT in germ free mouse to induce regulatory T cells (Treg) cells, and characterized immune regulatory activity in lamina propria of colon and small intestine. We found that oral feeding of B. bifidum IRT, and L. reuteri IRT significantly enhanced the generation of induced CD4+Foxp3+Helioslow Treg (itreg) cells and upregulated CTLA4 expression. Treatment of BMDCs and CD4+ T-cells treated with B. bifidum IRT, and L. reuteri IRT or their culture supernatants produced high amount of IL-10 in TLR-2 dependent manner. Currently we are investigating effector molecules from the probiotics strains. [This research was supported by grants from the Institute for Basic Science (IBS; IBS-R005-G1).] 82

84 Symposium [S8] : Probiotics and Gut Homeostasis S8-4 Application of Nutrition Related-metabolomics for Attenuating Metabolic Diseases Jong Ho Lee 1,2,3 *, Minjoo Kim 3, and Hyeon Yeong Ahn 3 1 National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University 2 Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University 3 Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University Metabolomics is a potentially useful approach for the exploration of disease, however, metabolic variation and the development of disease such as cardiovascular disease and hyperlipidemia have not been clarified. Thus, we evaluated the triglyceride (TG)-lowering effects of consuming dual probiotic strains of L. curvatus HY7601 and L. plantarum KY1032 on the fasting plasma metabolome. As a result, the TG-lowering effects of probiotic supplementation, partly through elevated apoa-v, in borderline to moderate hypertriglyceridemic subjects showed reductions in plasma metabolites; fatty acid primary amides and lysopcs. Probiotic supplementation with or without weight loss may protect against inflammation and adiposity. However, different probiotic strains even from the same species may have variable effects on fat distribution. Therefore, it remains to be established whether specific probiotic strains exert effects on fat accumulation and obesity. Thus, the effect of consumption of two probiotic strains, L. curvatus HY7601 and L. plantarum KY1032, on weight loss, body adiposity and lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ) activities in overweight subjects was examined. After 12-week probiotic treatment, the probiotic group presented reductions in body weight, body fat percentage and body fat mass measured using DEXA, and L1 subcutaneous fat area measured using CT, compared to baseline. The change in total fat mass correlated with change Lp-PLA 2, which correlated with change ox-ldl. Probiotic-induced weight loss was associated with reductions in fat mass, which correlated with the changes in Lp-PLA 2 activities. In addition, to determine changes in fasting metabolic intermediates with supplementation with a combination of L. curvatus HY7601 and L. plantarum KY1032 or placebo and evaluate whether changes in adiposity with probiotic or placebo supplementation were associated with changes in fasting metabolic intermediates. Consequently, in overweight individuals consuming a weight-maintenance diet, probiotic-induced weight loss and adiposity reduction in the supplementation of dual probiotic strains was associated with increases in medium chain acylcarnitines. 83

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86 Symposium [S9] Molecular Pathogenesis of Bacterial Pathogens Sponsored by Korea Research Institute of Bioscience and Biotechnology

87 2016 International Meeting of the Microbiological Society of Korea S9-1 Vibrio vulnificus RtxA1 Toxin as a New Target for Therapy Joon Haeng Rhee 1 *, Young Ran Kim 2, and Kyung Min Chung 3 1 Department of Microbiology, Chonnam National Unversity Medical School 2 College of Pharmacy, Chonnam National University 3 Chonbuk National University Medical School Vibrio vulnificus, a halophilic estuarine bacterium causing fatal septicemia and necrotic wound infection, produces a potent cytotoxin (RtxA1) of repeats in toxin family. The toxin kills host cells only after they come into contact with bacteria and plays an essential role in the pathogenesis. The 501-kDa RtxA1 toxin is processed into two fragments after its secretion into host cells. The larger N-terminal fragment (RtxA1-N, approximately 370 kda) remained at the host cell membrane, whereas the smaller C-terminal fragment (RtxA1-C, approximately 130 kda) was internalized into the host cell cytoplasm. RtxA1-N is believed to polymerize and form pores at the host cell. The RtxA1 toxin caused an increase in the intracellular Ca 2+ concentration and the subsequent activation of JNK. The cell death mechanism is via calcium-dependent mitochondrial pathways, resulting in irreversible mitochondrial membrane dysfunction and ATP depletion, and was later accompanied by the disruption of the integrity of the plasma membrane. Parts of RtxA1 protein appear to specifically interact with host proteins to bring cytotoxicity. Those RtxA1-host partner interaction could serve a new paradigm therapeutics and vaccine developments. 86

88 Symposium [S9] : Molecular Pathogenesis of Bacterial Pathogens S9-2 Vibrio MARTX Toxins Act as Effector Delivery Platforms to Inhibit Cellular Signaling during Infection Karla J.F. Satchell Department of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA Vibrio vulnificus causes rapid septicemia from contaminated seafood or wound infections and is notable for its high rates of hospitalization and death. It is a serious cause of disease in Korea and the US, as well as other countries. The most important virulence factor of V. vulnificus known to date is the large Multifunctional-Autoprocessing RTX toxin (MARTX). This large toxin is comprised of long repeat regions that function to form a pore for translocation of the central portion of the toxin across the eukaryotic cell plasma membrane. The translocated portion includes a cysteine protease domain that processes the toxin to release individual catalytically active effector domains. Although the translocation pore is also essential for cytolysis in vitro, this does not translate to virulence in mouse models, where the effector domains are essential for oro-gastric virulence. The function of many of these translocated domains is now recognized. Notably, the most highly virulent strains carry an effector domain that incapacitates the Ras-ERK pathway controlling cell proliferation and cytokine production by processing the Switch 1 region of Ras. Another domain has been found to function as a phospholipase A2 specific for phosphatyidylinositol- 3-phosphate blocking autophagy and endocytic trafficking. These newly characterized domains join previous studies characterizing domains as able to inhibit RhoGTPases, to induce mitochondrial-mediated apoptosis, and to produce adenylate cyclase. Thus, we have characterized the V. vulnificus MARTX toxin as a delivery platform for proteins that control virulence by modifying host cell signaling. 87

89 2016 International Meeting of the Microbiological Society of Korea S9-3 Structural Architecture of Multidrug Efflux Pumps and Type I Secretion Systems from Gram-negative Bacteria Jin-Sik Kim and Nam-Chul Ha* Department of Agricultural Biotechnology, CALS, Seoul National University The resistance-nodulation-division type tripartite pump AcrAB-TolC and its homologues are responsible for multidrug resistance in Gram-negative bacteria by expelling a wide variety of toxic substrates. The three essential components, AcrA, AcrB, and TolC, must function in concert with each respective binding partner within the complex. In this study, we report an 8.2 Å resolution cryo-electron microscopy 3D reconstruction of the complex that consists of an AcrAB fusion protein and a chimeric TolC protein. The pseudoatomic structure derived from the cryo-electron microscopy reconstruction clearly demonstratesa model only compatible with the adaptor bridging mechanism, wherein the funnel-like AcrA hexamer forms an intermeshing cogwheel-like interaction with the α-barrel tip region of TolC. These observations provide a structural milestone for understanding multidrug resistance in pathogenic Gram-negative bacteria, and may also lead to the design of new antibacterial drugs. We also present crystal structure of HlyD, which is a structural homologue of AcrA in the Type 1 secretion system and connects to TolC. Based on these structures, we discuss how two cylindrical channel proteins are connected in the Type 1 secretion system and the multidrug efflux pumps. [Supported by grants from NRF and ARC] 88

90 Symposium [S9] : Molecular Pathogenesis of Bacterial Pathogens S9-4 Horizontal Gene Transfer in Evolution of Mycobacterium tuberculosis towards Pathogenicity Olivier Neyrolles Institute of Pharmacology and Structural Biology, UMR5089 CNRS / Université P. Sabatier, Molecular Mechanisms of Mycobacterial Infections Department, France Several major pathogens, including the tuberculosis bacillus, Mycobacterium tuberculosis, parasitize host cells and exploit host-derived nutrients to sustain their own metabolism. Although the carbon sources that are used by M. tuberculosis have been extensively studied, the mechanisms by which mycobacteria capture and metabolize nitrogen, which is another essential constituent of biomolecules, have only recently been revisited. Here I will discuss central nitrogen metabolism in M. tuberculosis, the mechanisms that are used by this pathogen to obtain nitrogen from its host and the potential role of nitrogen capture and metabolism in virulence. In addition, a parallel transcriptional survey of intracellular mycobacteria and their host macrophages revealed signatures of heavy metal poisoning. In particular, mycobacterial genes encoding heavy metal efflux P-type ATPases CtpC, CtpG, and CtpV, and host cell metallothioneins and zinc exporter ZnT1, were induced during infection. Consistent with this pattern of gene modulation, we observed a burst of free zinc inside macrophages, and intraphagosomal zinc accumulation within a few hours postinfection. Zinc exposure led to rapid CtpC induction, and ctpc deficiency caused zinc retention within the mycobacterial cytoplasm, leading to impaired intracellular growth of the bacilli. Thus, the use of P(1)-type ATPases represents a M. tuberculosis strategy to neutralize the toxic effects of zinc in macrophages. We propose that heavy metal toxicity and its counteraction might represent yet another chapter in the host-microbe arms race. Altogether, our findings further highlight how nutrition and virulence are intimately linked in M. tuberculosis and other bacterial pathogens. 89

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92 Symposium [S10] Ecophysiology of Environmentally Important Microorganisms

93 2016 International Meeting of the Microbiological Society of Korea S10-1 Real-Time Detection and Sorting of Colorful Microbes Using Raman Microspectroscopy Tae Kwon Lee*, Ji Hyun No, Nishu Susmita, and Gui Nam Wee Department of Environmental Engineering, Yonsei University Microbes synthesize a diverse pigments which have a high potential of industrial applications. The industry is now able to produce some microbial pigments in the area of food, nutrition, pharmaceuticals and cosmetics. However, we overlook many of microbial resources possessing a novel biosynthetic pathway of pigment production due to its limited culture conditions. Thus, identification of useful microbes producing an interested valuable pigment without cultivation is the main technical challenge. To address this issue, we used Raman microspectroscopy to identify and sort living pigment producing bacteria from aquatic environment. For this purpose the lake sample was incubated for a few days under light sources. Subsequently, pigment producing microbes were directly identified via Raman microspectroscopy by their strong carotenoid peaks and sorted with an optical tweezer for whole genome amplification (WGA). Raman spectra obtained from these experiments were well separated into few groups, suggesting that microbial pigments were distinguishable with Raman microspectroscopy. This novel approach offers rapid and real-time detection of natural microbial pigment in the environmental samples, and a better understanding about ecophysiology of pigment producing microbes in the nature. 92

94 Symposium [S10] : Ecophysiology of Environmentally Important Microorganisms S10-2 Elucidating the Activity of Anaerobic Debrominating Bacteria: From Marine Sponges to Contaminated Sediments Max M. Häggblom Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA Microbial dehalogenation is central in determining the fate of organohalides in the environment and microorganisms appear to have evolved a variety of metabolic strategies for cleaving the carbon-halogen bond. One of the most intriguing metabolisms is the process of respiratory reductive dehalogenation in which the organohalide serves as the electron acceptor for anaerobic respiration. While aquatic sediments as significant sinks for halogenated organic pollutants, the marine environment is also a rich source of biogenic organohalides produced by a diversity of marine organisms. Marine sponges are known to produce a vast array of halogenated bioactive compounds as secondary metabolites. These organohalogen compounds in turn appear to select for bacteria that can utilize them as a source of energy. We have demonstrated that anaerobic organohalogen-respiring bacteria are widespread in different sponge species. From an Aplysina aerophoba sponge collected from the Mediterranean Sea we isolated a novel, strict anaerobic bacterium, Desulfoluna spongiiphila of the Deltaproteobacteria, that can grow by respiratory reductive debromination. Using a cultivation and molecular analysis-based approach we demonstrated that D. spongiiphila and its close relatives form a cosmopolitan group widely distributed in organohalide-containing sponges across different geographic locations. The sponge-associated dehalogenating bacteria can operate in vivo and impact the fate of brominated organics. Organobrominerich sponges thus appear to provide a specialized, possibly ancient, habitat for organohalide-respiring microbes, which mediate a cycling of organohalide compounds within the sponge animal. This new bacterial species group is an excellent model system to study a chemically-driven endosymbiotic relationship and one possible origin of organohalogen respiration. Understanding the microbial processes that control the fate and effects of organohalide compounds will in turn lay the foundation for harnessing the activities of dehalogenating bacteria in the development of novel bioremediation strategies. For example, stimulating anaerobic biological dehalogenation offers one of the most promising approaches towards eventual detoxification and complete degradation of halogenated contaminant mixtures. 93

95 2016 International Meeting of the Microbiological Society of Korea S10-3 Subsurface Microbial Redox Activities on Radionuclides in Contaminated Sediments Ji-Hoon Lee Department of Bioenvironmental Chemistry, Chonbuk National University Microbial activities on radionuclide elements, uranium and technetium, were investigated for their alteration in solubility and potentials on the immobilization by redox transformations, from the contaminated sediments, WA, USA. Influence of microbial and/or biogeochemical redox transformations of iron minerals was investigated on solubility of uranium (UO 2-2 ) and technetium (TcO - 4 ), and the contributing microbial communities were analyzed as well. Final products of the microbially and/or biogeochemically transformed phases of U and Tc were examined for the identification by using a variety of analytical advances. In addition, strains of lithoautotrophic bacteria with ability of Tc(VII) reduction were isolated and characterized from the oxygen gradient zone, suggesting the maintenance of the redox gradient in the subsurface transition zone and influence the migration of contaminants. 94

96 Symposium [S10] : Ecophysiology of Environmentally Important Microorganisms S10-4 Searching for Core Microbiome in Wastewater Treatment Plant Bioreactors Sang-Hoon Lee, Jeong-Hoon Park, and Hee-Deung Park* School of Civil, Environmental and Architectural Engineering, Korea University Core microbiome in activated sludge wastewater treatment bioreactors is important in interpreting the ecology of microbial consortia in the habitat. To search for core microbiome, we analyzed 16S rrna gene sequences collected temporally from 39 samples in 6 full-scale wastewater treatment plants located in Korea and China. In the seminar, I would like to present about several ecological behaviors of core microbiome observed in the treatment plants, such as patch dynamics, functional redundancy, and species sorting. This study will provide insight into the activated sludge bacteria that commonly occur. 95

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98 Symposium [S11] Lactic Acid Bacteria and Fermented Foods Sponsored by World Institute of Kimchi

99 2016 International Meeting of the Microbiological Society of Korea S11-1 Production of Lactobacillus brevis WK12, a Microbial Additive for Kimchi Fermentation, and Studies on Its Formulation Strategy Using Soy Powder and Microencapsulation Hae Woong Park*, Hyun Jung Gwak, Keon Jin Lee, Sang Il Lee, Sanghyun Ha, Ae Ri Han, Hyeyeon Song, Ho Hyun Chun, and Young Bae Chung R&D Division, World Institute of Kimchi Lactobacillus brevis WK12 is a potential microbial addictive which controls fermentation process to preserve the quality of kimchi. For industrial use of the strain, it is necessary to obtain high productivity of biomass and develop formulation strategy for viability. With optimal medium and operating conditions, the yield of CFU/ml was obtained in a 5 L jar. When fed with additional carbon source, the yield increased up to CFU/ml. Scale up of L. brevis WK12 production was successfully performed to a 5,000 L plant scale with a comparable yield of a 5 L jar. In addition, food-grade cryoprotective agents, i.e., skim milk, soy powder, yeast extract, and trehalose were tested for viability of L. brevis WK12 during freeze drying. Ten percentage of soy powder showed a strong protective effect on L. brevis WK12, showing 92.5% of viability. Microencapsulation in Ca-alginate beads enhanced the viability, compared to nonencapsulated cells. Moreover, there was a synergetic effect on the viability of 98% when encapsulated L. brevis WK12 in Ca-alginate beads were soaked in 10% of soy powder prior to freeze-drying. These results suggest that L. brevis WK12 may be ready for industrial use with cost effectiveness. 98

100 Symposium [S11] : Lactic Acid Bacteria and Fermented Foods S11-2 Metabolomics Understanding of Lactic Acid Bacteria during Fermentation Young-Shick Hong Division of Food and Nutrition, Chonnam National University Metabolomics is the study of metabolite profiling in multicellular systems of urine, serum and tissues of intact laboratory animals and patients. This field have exploded with new technologies during the past decade. Initially, metabolomics data were generated largely by NMR spectroscopy and more recently, has been studied using HPLC or UPLC combined with mass spectrometry (MS). The statistical analysis of multivariate metabolic data, especially in NMR-based metabolomics, make it easy to interpret the complex information in biological samples, e.g. drug toxicity, drug efficacy, lifestyle, age, gender, diet, and intestinal bacteria and parasites. NMR spectroscopy of plasma, urine, cerebrospinal fluid and urine as well as various tissues has been applied successfully to both preclinical and clinical studies of neurodegenerative disease such as Huntington disease, schizophrenia syndrome, muscular dystrophy and dietary modulation, evaluating the progression of disease and favourable versus unfavourable responses to drug or diet treatments. Because metabolomics is in many ways to closest to phenotype and physiology or pathology, and dietary effect, it might offer the best way to find out novel metabolic pathway and biomarker related to disease. This presentation introduces the basics of metabolomics and metabolomics understanding of various lactic acid bacteria during wine fermentation. 99

101 2016 International Meeting of the Microbiological Society of Korea S11-3 Health Claims for Probiotics and Prebiotics New Insights for Human Intervention Study to Measure Gut Inflammatory Response Ji Yeon Kim Department of Food Science and Technology, Seoul National University of Science and Technology An effectively functioning immune system is crucial for maintaining physiological integrity, and thus health. The immune system provides defense against infections caused by pathogenic microorganisms. Recently, EFSA published that maintaining a normal immune function is a beneficial physiological effect. Defense against pathogens comprises different mechanisms which act in concert to protect against infection. The presence of pathogenic microorganisms may cause infections at various sites of the body, and the defense against pathogens at a specific site of the body is considered a beneficial physiological effect. The capacity for defense against pathogens in the gastro-intestinal tract may be a good clinical trial model for proving gastro-intestinal health. Additionally, immune response to vaccination is an acceptable outcome to substantiate a beneficial physiological effect on the immune system. Inflammatory responsiveness or resilience to challenges may provide a more sensitive and meaningful indication of inflammatory state in the general population than the assessment of markers during the steady state and potentially the early response to a vaccine seem to be best standardized, most relevant and most feasible challenges for application in nutrition studies. Dietary modulation of the response to vaccination seems to be a good model for stimulating gastrointestinal inflammation and increasing gut permeability

102 Symposium [S11] : Lactic Acid Bacteria and Fermented Foods S11-4 Comparison of NGS Platforms for the Analysis of Korean Gut Microbiome Young-Do Nam Research Group of Gut Microbiome, Division of Nutrition and Metabolism Research, Korea Food Research Institute After the HGP, Human Microbiome Project (HMP) was initiated to fill a gap between our current understanding derived from HGP and actual physiological phenomenon not regulated by human but microbes and HMP created a new view of ourselves as super-organisms consisting of a human host and thousands of microbial symbionts. Human gut microbiota plays important roles in harvesting energy from the diet, stimulating the proliferation of the intestinal epithelium, developing the immune system, and regulating fat storage in the host. In addition, numerous diseases, including type 1 & 2 diabetes (T1 & T2D), inflammatory bowel disease (IBD), and gastric and colonic cancers, have been shown to be linked to dysbiosis of gut microbial communities. The shape of gut microbiota is mainly influenced by host genotype and diet style and Korean individuals have unique features in these two factors. Therefore, it is obviously assumed that the characteristics of Korean gut microbiota are differed from that of foreign populations. Recent advances of sequencing technology have been made more comprehensive analysis of microbiomes in various environments. However, there are some issues in the analysis of microbial diversity with these next generation sequencing (NGS) technologies. Before the analysis of Korean gut microbiome, NGS platform, target regions of marker gene, and databases for microbial identification should be evaluated. Therefore, we report the recent results of Korean gut microbiota analyzed with three types of NGS platforms covering different variable regions of 16S rdna gene

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104 Symposium [S12] Virus and Cancer

105 2016 International Meeting of the Microbiological Society of Korea S12-1 Mechanism of KSHV Latency and Cellular Transformation Shou-Jiang Gao Department of Molecular Microbiology and Immunology, University of Southern California, USA Kaposi s sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus causally associated with several human cancers including Kaposi s sarcoma (KS), primary effusion lymphoma and a subset of multicentric Castleman s disease. The life cycle of KSHV consists of latent and lytic replication phases. KSHV latent infection is required for the development of KSHV-associated cancers while lytic replication promotes the progression of cancers. Only a handful of KSHV genes are expressed during latency, which suppress viral lytic replication. A number of extracellular signals and downstream signaling pathways can reactivate KSHV into lytic replication. KSHV can efficiently infect and transform primary mesenchymal stem cells. KSHV-transformed cells are predominantly latent and can efficiently induce tumors in nude mice with pathological features highly reminiscent of human KS tumors. KSHV-encoded latent products including vflip (ORF71) and a cluster of micrornas are required for KSHV-induced cellular transformation by regulating cell growth and survival, and cellular metabolic pathways while vcyclin (ORF72) promotes cellular transformation by overriding p27-mediated contact inhibition. KSHV-transformed cells are addicted to a number of cellular pathways, which are potential therapeutic targets for KSHV-associated cancers

106 Symposium [S12] : Virus and Cancer S12-2 New Insights into the Biology of Hepatitis B Virus: Viral Oncogenesis Wang-Shick Ryu Department of Biochemistry, Yonsei University Chronic hepatitis B virus (HBV) infection leads to severe liver diseases such as cirrhosis and hepatocellular carcinoma (HCC). However, the mechanism underlying the HBV-associated HCC remained elusive. HBx, a viral regulatory protein, has been a focus, because HBx induces multiple cancer-related cytoplasmic signaling such as NF-kB, Ras-Raf-MAPK, and Wnt signaling. In addition, HBx-induced Myc stabilization has been reported; however, the mechanism by which HBx induces Myc stabilization has been unclear. We found that HBx induces Myc stabilization via directing binding on Myc oncoprotein. Mechanistically, the HBx-induced Myc stabilization is achieved by inhibiting the SCF Skp2 ubiquitin E3 ligase-mediated Myc ubiquitination. Moreover, we found the HBV-induced Myc elevation in HCC tissues, validating that HBx-induced Myc stabilization is attributable for the HBV-induced HCC. Importantly, we defined the Myc binding site of HBx polypeptide into four hydrophobic residues near the carboxyl-terminus (i.e., VFVL). Work is in progress to exploit this peptide segment for the treatment of the HCC

107 2016 International Meeting of the Microbiological Society of Korea S12-3 IFN-λ4 Potently Induces ISG15/USP18-mediated IFN-α Unresponsiveness Eui-Cheol Shin Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST IFN-λ4 is a newly identified type III IFN, and its genetic polymorphism has been proven to predict responses to IFN-α-based therapy in hepatitis C virus (HCV)-infected patients. The IFNL4-TT genotype, which does not produce IFN-λ4 protein due to a premature stop codon, is associated with a favorable treatment response while the IFNL4- G genotype coding for functional IFN-λ4 protein is associated with a poor treatment response. However, the mechanism of this paradoxic association has not been elucidated. Recently, we demonstrated that prolonged exposure to IFNs results in IFN-α unresponsiveness by ISG15 induction and USP18 stabilization (Sung et al. PNAS 2015, 112:10443). In the present study, we examined the ability of IFN-λ4 to induce ISG15/USP18-mediated IFN-α unresponsiveness in comparison with IFN-β and the other IFN-λs. We transfected Huh-7 cells with genes coding for various IFNs and examined the expression of ISG15 and USP18 and responsiveness to exogenous IFN-α treatment. After IFN-λ4 gene transfection, IFN-λ4 protein was scarcely detected in culture supernatant as previously reported. However, IFN-λ4 robustly increased the protein levels of ISG15 and USP18 in an IFN-λ R-dependent manner and potently reduced IFN-α responsiveness. The ISG15/USP18-mediated IFN-α unresponsiveness was confirmed by transfection of sirnas for ISG15 and/or USP18. This potent activity of IFN-λ4 was related with unphosphorylated ISGF3, formed by high levels of IRF-9, STAT-1 and STAT-2. The current data demonstrate that IFN-λ4 potently induces ISG15/USP18-mediated IFN-α unresponsiveness and explain why the presence of functional IFN-λ4 protein in IFNL4- G genotype-carrying hosts is associated with a poor response to IFN-α-based therapy against HCV infection

108 Symposium [S12] : Virus and Cancer S12-4 Constitutive Activation of T Cells by a Herpesviral GPCR through the Interaction with Cellular CXCR4 Eun-Kyung Kwon and Nam-Hyuk Cho* Department of Microbiology and Immunology, Seoul National University College of Medicine The members of herpesviral family use multiple strategies to hijack infected host cells and exploit cellular signaling for their pathogenesis. One of the intriguing arsenal of the pathogenic herpesviruses is the virally-encoded G protein-coupled receptors (vgpcrs) which are constitutively active and thereby harness host signaling pathways. Even though various cellular signaling could be modulated by the vgpcrs and contribute to viral pathogenesis such as immune evasion or proliferative disorders, molecular details how vgpcrs continuously activate cellular signaling is largely unknown. Here, we report that vgpcr of Herpesvirus saimiri (HVS), belonging to oncogenic 2-herepesviruses, constitutively activate T cells via a heteromeric interaction with cellular CXCR4 in the absence of any cognate ligand. Constitutive T cell activation also occurs by expression of vgpcr of Kaposi s sarcoma-associated herpesvirus (KSHV) but not by vgpcr of Epstein-Barr virus. Expression of HVS vgpcr down-regulated the surface expression of CXCR4 but did not induce degradation of the chemokine receptor, suggesting a continuous signaling in the cytosolic compartments. The physical association of the vgpcr with CXCR4 was demonstrated by proximity ligation assay as well as immunoprecipitation. Interestingly, ithe constitutive activation of T cells by HVS vgpcr is independent on the proximal T cell receptor (TCR) signaling molecules, such as TCR, Lck, or ZAP70, whereas CXCR4 silencing by shrna abolished T cell activation by vgpcrs of HVS or KSHV. Furthermore, inactive vgpcr mutants identified previously failed to interact with CXCR4. These findings on the positive cooperativity of vgpcr with cellular CXCR4 in T cell activation extend the current understanding of the molecular mechanisms regulating constitutive activity of vgpcr and shed light on the functional heteromerization for GPCR function

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110 Symposium [S13] Human Opportunistic Fungal Pathogens Sponsored by BK21PLUS Initiative for Biological Function & Systems

111 2016 International Meeting of the Microbiological Society of Korea S13-1 Dimorphism and Host-Pathogen Interactions in the Emerging Fungal Infection, Mucormycosis Soo Chan Lee 1 *, Judith M. Bain 2, Johanna Louw 2, Lars P. Erwig 2, Dennis C. Ko 1, and Joseph Heitman 1 1 Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA 2 Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK The host-pathogen interface is an important base in establishment of infections by pathogenic microbes and understanding these processes provides a fundamental forum to develop therapeutic interventions. Although mucormycosis, an infection caused by mucoralean fungi, is emerging and continuing to increase due to increasing cohorts of immunocompromised patients, our knowledge about host-pathogen interactions in mucormycosis is as yet in its infancy. The causal fungi include Mucor spp., Rhizopus spp., Lichtheimia spp., and others, among which this study focuses on Mucor circinelloides. Interestingly, Mucor is a dimorphic fungus and its morphogenic transition between spores/hyphae and yeast depends on environmental conditions. We found that the spores/hyphae are more virulent form of this fungus; in contrast, yeast-locked mutants display significantly diminished virulence. In this study, we further demonstrate that the dimorphic transition of Mucor programs different outcomes during host-pathogen interactions. When macrophages phagocytose Mucor yeast, subsequent phagosomal maturation occurs, indicating host cells respond appropriately to control the pathogen. On the other hand, upon phagocytosis of spores, macrophages fail to form mature phagosomes. The proangiogenic growth factor FGF-2 is induced from cultured and primary immune cells by spores/hyphae but not by yeast, and proinflammatory cytokines are induced in cultured human monocyte cells by spores/hyphae but not by yeast. Interestingly, spores of another mucoralean fungus, Rhizopus, display similar host-pathogen interactions, including phagosome maturation arrest and induction of FGF-2 from cultured human immune cells. The foundation provided by Mucor dimorphism will further enable us to identify general host-pathogen interactions that are deployed by Mucorales fungi, thus facilitating the development of therapeutic interventions against this fatal fungal infection

112 Symposium [S13] : Human Opportunistic Fungal Pathogens S13-2 Systematic Functional Analysis of Pathogenicity Networks in a Global Fungal Meningitis Pathogen Yong-Sun Bahn Department of Biotechnology, Center for Fungal Pathogenesis, Yonsei University Cryptococcus neoformans causes life-threatening meningoencephalitis in humans, but the treatment of cryptococcosis remains challenging. To develop novel therapeutic targets and approaches, signaling cascades governing pathogenicity of C. neoformans have been extensively studied but the underlying pathobiological regulatory circuits remain elusive. In this study, we constructed a high-quality library of more than 550 signature-tagged gene-deletion strains through homologous recombination methods for 155 putative transcription factor and 117 kinase genes and examined their in vitro and in vivo phenotypic traits under 32 distinct growth conditions. This high-functional-coverage phenome analysis uncovered myriad novel transcription factors and kinases, which play critical roles in growth, differentiation, stress responses, antifungal drug resistance, and virulence. Large-scale virulence and infectivity assays in insect and mouse host models identified more than hundred genes that are critical for pathogenicity. These pathogenicity-related transcription factors and kinases are involved in the following biological functions: growth and the cell cycle, nutrient metabolism, the stress response and adaptation, cell signalling, cell polarity and morphology, vacuole trafficking, trna modification, and other previously unknown functions. The genotypic and phenotypic data for each transcription factor and kinase are all publicly available in the C. neoformans transcription factor phenome database and kinase phenome database, respectively. In conclusion, our phenome-based functional analyses of the C. neoformans transcription factor and kinase mutant libraries provide key insights into regulatory networks of basidiomycetous fungi as well as the ubiquitous human fungal pathogen

113 2016 International Meeting of the Microbiological Society of Korea S13-3 Synthesis and Regulation of Zearalenone in Fusarium graminearum Yin-Won Lee* and Ae Ran Park Department of Agricultural Biotechnology, Seoul National University Some Fusarium species produce zearalenone (ZEA) that is a polyketide mycotoxin. ZEA causes hyperestrogenic syndrome in animals and is often found in F. graminearum infected cereals. The ZEA biosynthetic cluster genes PKS13, PKS4, ZEB1 and ZEB2 encode a non-reducing polyketide synthase, a reducing polyketide synthase, an isoamyl alcohol oxidase and a transcription factor, respectively. In particular, the ZEB2 gene produces two isoforms (ZEB2L and ZEB2S) via an alternative promoter. ZEB2L contains a basic leucine zipper (bzip) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bzip domain. ZEB2L and ZEB2S interact with each other to form a heterodimer that regulates ZEA production by reducing the binding affinity of ZEB2L for the ZEB2L gene promoter. The ZEB2 expression is autoregulated by alternative promoter usage for ZEA production in F. graminearum; this regulatory mechanism is similar to that in higher eukaryotes

114 Symposium [S13] : Human Opportunistic Fungal Pathogens S13-4 The NDR Kinase Cbk1: A Versatile Player in the Hyphal Morphogenesis of Candida albicans Jong-Myeong Kim, Hye-Jeong Lee, Woo-Kyu Kang, and Jeong-Yoon Kim* Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University NDR (nuclear Dbf2-related) kinases, which belong to the serine/threonine AGC (PKA/PKG/PKC-like) class of protein kinases, are evolutionarily conserved from yeast to human. The NDR kinase Cbk1 is the key component of the RAM (Regulation of Ace2 and Morphogenesis) signaling network that regulates diverse cellular processes, including daughter cell-specific gene expression, cell wall integrity, and glycosylation and secretion, in Saccharomyces cerevisiae. Recently, we reported that the NDR kinase Cbk1 and other components in the RAM signaling network are essential for the hyphal morphogenesis of the human fungal pathogen Candida albicans. In addition, C. albicans Cbk1 was found to be associated with maintenance of cell wall integrity, completion of cell separation, and induction of ergosterol biosynthesis genes in response to hyphal induction or azole drug treatment. These results suggest that Cbk1 should be one of the most important proteins involved in the pathogenicity of C. albicans. To unravel the molecular mechanisms by which Cbk1 controls C. albicans hyphal morphogenesis, we investigated the roles of Cbk1 downstream effectors in hyphal growth. We found that the mrna-binding protein Ssd1 is a substrate of Cbk1 and the phosphorylation of Ssd1 by Cbk1 is required for the degradation of the transcriptional repressor Nrg1 during hyphal initiation. Identification of Ssd1-bound mrnas suggested that Ssd1 is involved in the activation of the camp/pka signaling pathway that promotes the hyphal growth of C. albicans by degrading Nrg1 and activating the transcriptional activator Efg1. We also found that, while the Cdc42 GTPase is widely distributed over the cytoplasm in the absence of Cbk1, a constitutively active form of Cdc42 is localized at the growing bud and hyphal tip in the cbk1 mutant, which suggests that Cbk1 plays a role in the localization of Cdc42 in C. albicans. We will discuss how Cbk1 orchestrates diverse cellular processes required for the polarized growth of C. albicans

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116 Symposium [S14] Synthetic Microbiology for Biotechnology and Therapeutic Applications

117 2016 International Meeting of the Microbiological Society of Korea S14-1 Microbial Cell Factory for Isoprenoids Production Chonglong Wang, Jung-Hun Kim, Ji-Bin Park, Ju-Eon Park, and Seon-Won Kim* Division of Applied Life Science (BK21 Plus), PMBBRC, Gyeongsang National University Isoprenoids, also called terpenoids, are a large and diverse class of naturally-occurring compounds. They are present in all living organisms and include many important drugs, valuable flavor and fragrance compounds, pigments, antioxidants, steroids and natural polymers. However, most of valuable isoprenoids are produced in a trace amount as secondary metabolites from intractable slow growing organisms such as plants, actinomycetes, archaea, etc. Isoprenoids are derived from five-carbon universal building blocks assembled and modified in various ways. IPP (isopentenyl diphosphate) and DMAPP (dimethylallyl diphosphate) are the building blocks which are produced from either the mevalonic acid (MVA) or methylerythritol phosphate (MEP) pathways. The increased synthesis of building blocks of IPP and DMAPP through metabolic redesign is a way to enhance the production of isoprenoids. Using E. coli as a host, IPP and DMAPP supply can be increased significantly through the introduction of foreign MVA (mevalonate) pathway into it. The carotenoids are tetraterpenoid pigment molecules, facilitating metabolic redesign for the pathway optimization because of their convenient colorimetric screening properties. Because the root of all isoprenoids synthesis pathway share the universal C5 metabolic precursors (IPP and DMAPP), metabolic redesign work with carotenoids can provide genetic platforms for the production of other valuable isoprenoids. We successfully produced the valuable compounds of isoprene, geraniol, farnesol, farnesene, squalene, santalene, bisabolol and retinoids using the platform E. coli strain resulting from the carotenoids works. [This work is supported by a grant (NRF-2013R1A1A ) and a grant (NRF-2012M1A2A ) from the National Research Foundation, MSIP, and a grant from the Next-Generation BioGreen21 Program (SSAC, grant#: PJ ), RDA, Korea] 116

118 Symposium [S14] : Synthetic Microbiology for Biotechnology and Therapeutic Applications S14-2 Evolutionary Engineering for Chemical Producing Microorganisms Using Synthetic Regulators Jina Yang 1, Sungho Jang 1, and Gyoo Yeol Jung 1,2 * 1 Department of Chemical Engineering, POSTECH 2 I-Bio Program, POSTECH Pathway optimization of microbial metabolism is essential for the production of commercially valuable chemicals such as biofuels, platform chemicals and biologically active compounds. To achieve the successful design or redesign of microbial metabolism, robustness of naturally occurring biological systems has to be relieved so that cells can be easily redesigned. Although extremely huge efforts have been made to find genetic target to improve metabolic function of the microorganisms, there still exists the additional room for the non-rational approach. Currently, typical approach for metabolic engineering uses both rational approach as well as non-rational methods such as combinatorial and evolutionary methods. One of the most critical problems of metabolic engineering is especially robustness of the biological systems. Bacterial cells are generally evolved at the various levels from DNA to protein for maintaining their robustness against the changing circumstances. Therefore, general strategy to modify cellular physiology depending the robustness or flexibility of the biological systems should be required. In this study, we developed synthetic selection devices using the expression regulators at transcription and translation levels. By combining the product-sensitive promoters and selection markers, a selection device turning the cellular phenotype into selective based on the intracellular concentration of the product. Additionally, intracellular metabolite sensor named riboselector to regulate metabolic distribution will be presented. The potentials of the platform technology developed in this study for the application to the production of biofuels and commodity chemicals

119 2016 International Meeting of the Microbiological Society of Korea S14-3 Bacteria-mediated Cancer Theranostics: Bacteria Meet Oncology Jin Hai Zheng 1, Seung-Hwan Park 1, Yeongjin Hong 2, Joon Haeng Rhee 2, Hyon E. Choy 2, and Jung-Joon Min 1,2 * 1 Department of Nuclear Medicine, 2 Department of Microbiology, Chonnam National University Medical School The word theranostics refers to the simultaneous integration of diagnosis and therapy. Cancer theranostics is to apply and further develop anti-cancer strategies for advanced theranostics, i.e. to apply and further develop the various carriers such as microbes, protein scaffolds, polyer conjugations, and other organic/inorganic nanoparticles for sustained, controlled and targeted co-delivery of diagnostic and therapeutic agents. Bacteria have unique properties that make them well-suited for use as optimized anticancer agents. This is because bacteria can specifically target and proliferate in cancer tissue and can be engineered to overcome the limitations that hamper current cancer therapies. Using a top-down engineering approach, the ideal cancer therapy can be envisioned as follows: bacterial cells are engineered as tiny programmable micro-sized robot that specifically target tumors, move in response to external signals, induce specific cytotoxicity and are externally detectable in vivo. Our work provides a basis to develop novel targeted cancer theranostics that may complement conventional therapy in the future

120 Symposium [S14] : Synthetic Microbiology for Biotechnology and Therapeutic Applications S14-4 Engineered Biosynthesis of Non-immunosuppressive FK566 Analogues with Improved Therapeutic Properties Yeo Joon Yoon Department of Chemistry and Nano Science, Ewha Womans University FK506 is a 23-membered macrolide of Streptomyces origin exhibiting various biological activities such as immunosuppressive, antifungal, neuroprotective, and neuroregenerative. It is a clinically important drug used to prevent the rejection of organ transplants. We recently characterized the detailed biosynthetic pathway of allylmalonyl-coenzyme A from which the FK506 allyl group is derived. Characterization of this discrete pathway facilitated the engineered biosynthesis of novel allyl group-modified FK506 analogues which exhibits improved neurite outgrowth activity. We have also detailed the post-modification step in the biosynthesis of FK506, demonstrating that substrate-flexible post-pks modification enzymes can be utilized for the biosynthesis of non-immunosuppressive FK506 analogues with neuroregenerative and antifungal activity. In addition, FK506 analogues containing a non-natural starter unit could be obtained through mutasynthesis. Taken together, these results illuminates an efficient synthetic biology strategy for the generation of FK506 analogues with improved therapeutic properties

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122 Symposium [S15] Ecological Aspect of Microorganisms via Phylogenetic Approach Sponsored by CFST, Seoul National University

123 2016 International Meeting of the Microbiological Society of Korea S15-1 Systematics of Vibrionaceae Based on Analysis of Genome Sequence Data Henryk Urbanczyk Faculty of Agriculture, Department of Marine Biology and Environmental Sciences, University of Miyazaki, Japan This presentation will describe studies of diversification of evolutionary closely related Vibrio species (family Vibrionaceae, Gammaproteobacteria). Initially, whole genome sequence data was used for classification of the Vibrio strains into species. Later, the number of interspecies and intraspecies recombination events occurring within core genomes of the bacteria was estimated. The analysis revealed a low number of interspecies recombination events when analyzing strains isolated from different ecologies, over 80 years apart, or from different hemispheres. Similarly, the number of identified interspecies recombination events was low between strains isolated from the same geographic location within a short time frame. In contrast, the number of identified intraspecies recombination events was disproportionally high, even between strains that have significant temporal (over 18 years) and geographical (over 10,000 km) differences in their origins of isolation. Results of this study reveal a remarkable stability of the analyzed Vibrio species, suggest that ecology of bacteria had little influence over the frequency of interspecies recombination in the core genomic regions, and give clues about the origins and persistence of Vibrio species

124 Symposium [S15] : Ecological Aspect of Microorganisms via Phylogenetic Approach S15-2 Microbiome Studies in Various Samples Bong-Soo Kim Department of Life Sciences, Hallym University The advancements of sequencing techniques with developing bioinformatic tools have been applied to the various microbiome studies in environments. To date, high-throughput sequencing of amplified gene marker (eg. 16S rrna) for phylogenetic approach has been widely used to study of microorganisms in environments. These approaches have provided the structure of microbial communities in environments; however, the ecological interpretations with taxonomic information of microbial communities are limited. Therefore, metagenome shotgun sequencing and metatranscriptome sequencing have been used to analyze the functional roles of microbial community in these days. These techniques can provide both of taxonomic and functional profiles of microbial communities. We applied high-throughput sequencing to study microbiome in various environmental samples. Here, we briefly introduce our studies on various samples

125 2016 International Meeting of the Microbiological Society of Korea S15-3 Characterization of Extremely Halophilic Microbial Eukaryotes (Protozoa): Autecology and Diversity Jong Soo Park Department of Oceanography, School of Earth System Sciences, Kyungpook National University Hypersaline environments are widely, but sparsely distributed across Earth. Representative hypersaline habitats above 150 salinity are Dead Sea, Great Salt Lake, Hutt Lagoon, Wieliczka salt mine, and solar salterns as a type of extreme environment. All domains of life are capable of growth under these extreme conditions. Halophilic bacteria are known to be ~50 genera including Salinibacter and Halomonas, while halophilic archaea are spread ~20 genera, mostly in the Haloarchaea as a single clade. The best known microbial eukaryote in hypersaline environments is autotrophic Dunaliella spp., which are extreme halotolerant algae. However, the autecology and diversity of microbial eukaryotes are more poorly understood, in particular for heterotrophic eukaryotes (protozoa). In fact, many scientists suggest that there is no heterotrophic eukaryote among extreme halophiles. Here, several heterotrophic microbial eukaryotes are successfully isolated from a variety of hypersaline environment: Halocafeteria, Pleurostomum, Trimyema, Tulamoeba, Euplaesiobystra, and Pharyngomonas. All isolates are the previously unknown genera or species that can grow at 150 salinity or above. Also, SSU rrna gene sequences of the cultured protozoa isolated from hypersaline waters of >125 salinity in Australia, North America, and Europe (6 geographic sites, 25 distinct samples) imply that their biogeographic distribution may be depended on the size of cells in some cases. Therefore, hypersaline environments can harbor lots of the novel species, but more-specific molecular detection of halophilic protozoa is still needed to test their biogeographic pattern across Earth

126 Symposium [S15] : Ecological Aspect of Microorganisms via Phylogenetic Approach S15-4 Platforms for Analysis of Fungal Diversity and Application to the Antarctic Environments Soon Gyu Hong 1 *, Kyung Mo Kim 2, Kyuin Hwang 1, Jeongsu Oh 2, Ok Sun Kim 1, Hyun Soo Lim 3, Ahn Na Cho 1, Hyun Joo Noh 1, Yung Mi Lee 1, and Hong Kum Lee 1 1 Division of Polar Life Sciences, Korea Polar Research Institute 2 Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology 3 Department of Geological Sciences, Pusan National University Fungi are important components of the nature and information for fungal diversity in each habitat is a prerequisite to understand nutrient cycling and biological activities in soil, rock, and water, and physiological activities of plants and animals that are affected by fungal symbiosis and pathogenicity. We developed a series of bioinformatics tools to analyze fungal diversity from NGS sequence information, which includes MycoDE, a reference sequence database with curated taxonomic information. The Antarctic is one of the least studied areas in the world and fungal diversity is hardly known. Fungal diversity of Antarctic environments was analyzed from 1,700,000 LSU rrna gene sequence reads obtained by amplicon sequencing from terrestrial soil, marine sediment, rock, fresh water, seawater, biofilm, plant, and lichen samples. Clustering sequences altogether with 99% similarity cutoff resulted in 26,000 OTUs. The highest fungal diversity was observed from terrestrial soil samples and followed by marine sediments and plant samples. Large proportion of fungal OTUs recovered from fresh water, plant and marine sediments were unique to each habitat. Instead fungal OTUs in lichen samples were frequently found in terrestrial soil, and marine sediment. Many of rock inhabiting fungi were also found in terrestrial soil and lichen samples. The majority of fungi were included in Ascomycota and Basidiomycota, and especially in classes Dothideomycetes, Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Saccharomycetes, and Sordariomycetes in Ascomycota and in classes Agaricomycetes, Pucciniomycetes, Tremellomycetes and Rhodotorula related phylogenetic lineages in Basidiomycota. Many of the fungal OTUs were still unclassified by sequence similarity searches, implying that there are a lot of uncovered fungal species in the Antarctic environments. Habitat sharing and geographical migration of fungal species will be presented

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128 Symposium [S16] Recent Progress in Vaccine Development against Traditional and Emerging Pathogens Sponsored by International Vaccine Institute

129 2016 International Meeting of the Microbiological Society of Korea S16-1 Universal Influenza Vaccine Approach: Options and Obstacles Baik Lin Seong 1,2,3 * and Yo Han Jang 1 1 Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University 2 Translational Vaccine Research Center, Yonsei University 3 Translational Research Center for Protein Function Control, Yonsei University The recent discovery and characterization of neutralizing antibodies specific to the highly conserved stalk (stem) region of the influenza virus hemagglutinin (HA) has raised an exciting prospect that a universal influenza vaccine could be developed by rational vaccine designs. To achieve this goal, prime-boost immunization strategy with 'chimeric HA' or 'headless HA' has been advanced to redirect host immune responses from the variable globular head domain to the conserved stalk domain. While this approach has been successful in eliciting cross-reactive antibodies against the HA stalk domain, protective efficacy remains relatively poor due to low immunogenicity of the domain, and the cross-reactivity was only within the same group, rather than among different groups. Additionally, concerns are raised on the possibility of vaccine-associated enhancement of viral infection, and whether multiple boost immunization protocols would be considered practical from a clinical standpoint. A live attenuated influenza vaccine (LAIV) hitherto remains unexplored, but is expected to serve as an alternative approach considering its well-known superior cross-reactivity. The LAIV mimics natural infection and thus provide efficient protection against the viral infection by inducing both systemic and local immunity. Well-documented cross-protection of the LAIV over the inactivated or subunit vaccines could serve the key toward the development of the universal influenza vaccine. Our data showed that prime-boost vaccinations with various combinations of cold-adapted live attenuated influenza vaccines induced cross-reactive systemic and mucosal antibody responses against the heterologous influenza viruses. Notably, all the immunized mice were protected against lethal challenges with the heterologous and heterosubtypic HA group 1 and group 2 viruses, even in the absence of antibody-mediated viral-neutralization, membrane fusion inhibition, or antibody-dependent cell-mediated cytotoxicity in vitro assays. Instead, specific cytotoxic T-cell responses directed against to the conserved NP or HA epitope were stimulated upon the heterologous infections, suggesting that the CTL responses may play an important role in the cross-protection. The results suggest that the LAIVs provide a simple and alternative option for developing the truly universal influenza vaccines with broad spectrum of protection covering both HA groups of influenza viruses

130 Symposium [S16] : Recent Progress in Vaccine Development against Traditional and Emerging Pathogens S16-2 Human DPP4 Transgenic Mice as Surrogate Models for MERS Chien-Te K. Tseng 1,4 *, Anurodh Shankar Agrawal 1, Xinrong, Tao 1, Abdullah Algaissi 1, Tania Garron 1, Tehsheng Chan 1, Bi-Hung Peng 2, and Robert B. Couch 3 1 Departments of Microbiology and Immunology, 2 Pathology, 3 Internal Medicine, Division of Infectious Disease, and 4 Center for Biodefense and Emerging Infectious Disease, University of Texas Medical Branch, Galveston, Texas, USA Continued occurrences of the Middle East Respiratory Syndrome caused by a coronavirus (MERS-CoV) and its proven transmissibility among humans constitute an ongoing public health threat. Animal models, especially small animal models that simulate human disease, are needed for studies of pathogenesis and development of vaccines and antivirals for prevention and treatment of MERS-CoV infection and disease. Mice and other commonly used laboratory small animal species (i.e., hamsters and ferrets) are not susceptible to MERS-CoV because they lack the dipeptidyl peptidase 4 (DPP4) viral entry receptor. To overcome this deficiency, we developed several lineages of transgenic mice expressing human (h) DPP4 globally or specifically within lungs by using the CAGGS and surfactant protein B (SPB) promoters, respectively, as surrogate models for MERS-CoV infections. We showed that one lineage (line 52) of transgenic mice globally expressing hdpp4 is highly susceptible to intranasal challenge with MERS-CoV as extensive viral infection developed within lungs and brain and dissemination occurred to other organs; relentless weight and uniform death ensued; and the 50% lethal dose (LD 50 ) and infectious dose (ID 50 ) were 5 and 0.4 TCID 50 of MERS-CoV, respectively. Additionally, the model provided a robust preclinical model for testing the efficacy of medical countermeasures for MERS. In contrast to the ubiquitous hdpp4 expression of CAGGS-derived transgenic mice, three (out of twelve) SPB-hDPP4 transgenic lineages (Lines 20, 71, and 87) expressed hdpp4 almost exclusively within the lungs but with different intensity. Consistent with this lung-specific hdpp4 expression, active viral infection appeared restricted to the lungs as virus was not detected in the extra-pulmonary tissues tested. Although the lowest hdpp4 expression was for line 87, it was the first with sufficient numbers of animals for further testing. These mice were challenged intranasal with MERS-CoV (10 6 TCID 50 /mouse). Viral infection was restricted to the lungs of 3 animals harvested at days 3 and 6 post infection (p.i.). The remaining challenged mice (N=9) exhibited varying degrees of clinical illness (weight loss), ranging from severe (N=2), to mild/moderate (N=4), to asymptomatic (N=3). All were infected as specific antibodies were readily detected in all at day 21 p.i. Additional characterization of these global and lung-specific hdpp4 transgenic mice is ongoing to establish attractive small animal surrogate models of MERS-CoV infection and disease for furthering knowledge of pathogenesis and for development of effective vaccines and treatments for MERS

131 2016 International Meeting of the Microbiological Society of Korea S16-3 In Vivo Molecular Imaging Analysis of Vaccine Candidates in a Mouse Model Hyewon Youn 1,2,3 1 Department of Nuclear Medicine, 2 Cancer Research Institute, Seoul National University College of Medicine 3 Cancer Imaging Center, Seoul University Hospital In vivo molecular imaging is one of the most powerful tools to investigate biologic events in living animals. By taking advantage of non-invasive bioluminescence imaging using luciferase expressing splenocytes, we monitored the enhancement of immune response against hepatitis B virus antigen with adjuvant vaccination in real time whole body imaging. To visualize vaccinated antigen, hepatitis B virus antigen (HBsAg) was labeled with radioiodine ( 125 I-HBsAg) and monitored for 5 weeks. B6 mice were vaccinated intramusculary with 125 I-HBsAg, 125 I-HBsAg+adj1 and 125 I-HBsAg+adj1+adj2. The localization of vaccinated HBsAg was monitored using animal Single Positron Emission Computed Tomography (SPECT)/CT. Localization of vaccinated HBsAg was successfully monitored using animal SPECT/CT. HBsAg was lasted for 5 weeks and diminished. In addition, the injected splenocytes were successfully visualized from vaccinated mouse homing to primary target organs and accumulated in cervical, axillary, mesenteric, inguinal lymph nodes within 5 h. Vaccinated mouse with adjuvants ( 125 I-HBsAg+adj1+adj2) showed 2 times more accumulation of splenocytes at vaccination site compare to vaccinated mouse with antigen only ( 125 I-HBsAg). Six days later, vaccinated mouse with two adjuvants showed fold increased luciferase intensity of splenocytes at spleen, lymphoid organs and vaccination site compare to vaccinated mouse with antigen only. In vivo real-time monitoring system successfully provides the localization of vaccine candidates and efficiency of adjuvants in early time point

132 Symposium [S16] : Recent Progress in Vaccine Development against Traditional and Emerging Pathogens S16-4 Vaccine Development Program for Developing Countries, International Vaccine Institute ManKi Song Clinical Research Lab. Department, Science Unit, International Vaccine Institute, SNU Research Park The International Vaccine Institute (IVI) is an international nonprofit organization that was founded on the belief that the health of children in developing countries can be dramatically improved by the use of new and improved vaccines. Working in collaboration with the international scientific community, public health organizations, governments, and industry, IVI is involved in all areas of the vaccine spectrum from new vaccine design in the laboratory to vaccine development and evaluation in the field to facilitating sustainable introduction of vaccines in countries where they are most needed. IVI's Laboratory Sciences programs are dedicated to vaccine research, development, technical assistance and technology transfer. IVI lab scientists work on: Basic and applied research to design vaccines; Technical assistance programs for developing country researchers, producers, and regulatory authorities; and Transfer of vaccine manufacturing processes and related technologies to qualified vaccine producers in developing countries Development of vaccines for Shigella, MERS, Zika, Norovirus, cholera and typhoid Development of vaccine evaluation system for human clinical trials

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134 Symposium [S17] Signal Transduction and Gene Regulation in Bacteria

135 2016 International Meeting of the Microbiological Society of Korea S17-1 H 2 O 2 Sensitivity of Metal-dependent Peroxide Sensor PerR Chang-Jun Ji, Jung-Hoon Kim, and Jin-Won Lee* Department of Life Science, Hanyang University In many Gram positive bacteria PerR is a major peroxide sensor whose repressor activity is dependent on a bound metal cofactor. The prototype for PerR sensors, the Bacillus subtilis PerR BS protein, represses target genes when bound to either Mn 2+ or Fe 2+ as corepressor, but only the Fe 2+ -bound form responds to H 2 O 2. The orthologous protein in the human pathogen Staphylococcus aureus, PerR SA, plays important roles in H 2 O 2 resistance and virulence. However, PerR SA is reported to only respond to Mn 2+ as corepressor, which suggests that it might rely on a distinct, iron-independent mechanism for H 2 O 2 -sensing. Here we demonstrate that PerR SA uses either Fe 2+ or Mn 2+ as corepressor, and that, like PerR BS, the Fe 2+ -bound form of PerR SA senses physiological levels of H 2 O 2 by Fe-mediated histidine oxidation. Moreover, we show that PerR SA is poised to sense very low levels of endogenous H 2 O 2 which normally cannot be sensed by B. subtilis PerR BS. This hypersensitivity of PerR SA accounts for the apparent lack of Fe 2+ -dependent repressor activity and consequent Mn 2+ -specific repressor activity under aerobic conditions. Furthermore, we also show that mutations at regulatory metal binding site can affect the metal-ion specificity and H 2 O 2 -sensitivity of PerR BS and PerR SA

136 Symposium [S17] : Signal Transduction and Gene Regulation in Bacteria S17-2 'Oxygen-FNR-sRNA Regulatory Pathway' Controls the Anaerobic Induction of Fermentation-Respiration Switch Protein Bo-Ram Jang, Kyung-Jo Lee, and Kyu-Ho Lee* Department of Life Science, Sogang University Fermentation respiration switch (FrsA) is an enzyme catalyzing a conversion of pyruvate to acetaldehyde and carbon dioxide. FrsA protein level was not detectable in Vibrio vulnificus cells grown under oxygen-rich condition, and thus the in vivo activity of pyruvate decarboxylation derived from FrsA was observed in the cells grown under oxygen-limited condition. To investigate the regulatory mechanism(s) for the anaerobic induction of FrsA expression and activity, its transcription was monitored using both frsa-transcription reporter and quantitative RT-PCR assays. However, no significant difference was observed in its transcription and the resultant transcripts in the cells grown under aerobic or anaerobic conditions. This result lead us to consider the specific regulation at the post-transcription level and to examine the involvement of srna in FrsA expression. A candidate regulatory srna for FrsA expression (Rsf), including the sequences complementary to the 5'-UTR of frsa mrna, was identified in V. vulnificus genome. A northern blot revealed the presence of 350 nucleotide-long srna. Its regulatory role was examined via monitoring FrsA levels in the rsf-deleted mutant V. vulnificus. In the absence of rsf gene, the negative effect of oxygen on the cellular level of FrsA was abolished, and thus the rsf mutant exhibited high activity of pyruvate decarboxylation even under the aerobic condition. It was further determined the regulatory dependency of Rsf on oxygen in repressing FrsA expression. Expression of the rsf gene was repressed by a transcription factor FNR under anaerobic condition, whereas repression of rsf transcription by FNR was relieved in the presence of oxygen. Thus, this study demonstrates that the cellular content of FrsA is minimized during aerobic growth via repression of its expression by Rsf. This repression, however, is relieved under anaerobic condition via repression of the rsf transcription by FNR, resulting in higher levels of the cellular FrsA and the mixed-acid fermentative metabolisms

137 2016 International Meeting of the Microbiological Society of Korea S17-3 Three Mechanisms of Oxygen Sensing in Mycobacteria: Regulation of Gene Expression in Response to Changes in Oxygen Tensions Jeong-Il Oh Department of Microbiology, Pusan National University Mycobacterium tuberculosis (Mtb) is an obligatory aerobic bacterium that is causative of tuberculosis. Mtb can survive in a non-replicating, persistent form in the immune-competent host and establish latent infection without exhibiting any symptoms. When Mtb is phagocytosed by host macrophages, it encounters hostile environments such as hypoxic, low ph, and nutrient-limiting conditions as well as reactive oxygen species (ROS)- and reactive nitrogen species (RNS)-challenging conditions, which is thought to lead to latency transition of Mtb. Since gradual depletion of oxygen from mycobacterial cultures was shown to lead to the transition of their growth state to a latency-like state, low oxygen tension was suggested to be one of the most plausible determinants for latency transition of mycobacteria. Mycobacteria possess several exquisite regulatory systems that are involved either directly or indirectly in oxygen sensing. The DosSR (DevSR) two-component system is the most important regulatory system pertinent to the hypoxic adaptation of mycobacteria. In Mtb the DosR response regulator is phosphorylated by the paralogous DosS and DosT histidine kinases that contain b-type heme in their N-terminal GAF-A domains. The kinase activity of DosS and DosT was shown to be controlled by the ligand-binding and redox state of the heme iron. The unliganded ferrous (deoxyferrous) form of the HKs is active to phosphorylate DosR, while the O 2 -bound (oxyferrous) and ferric forms are inactive. The functionality of the respiratory electron transport chain is altered in response to changes in oxygen tensions, which is reflected to regulate gene expression by the transcriptional factor such as AldR in mycobacteria. The cellular level of camp in mycobacteria is drastically increased under hypoxic and respiration-inhibitory conditions. In response to changes in camp level, CRP (camp Receptor Protein) modulates gene expression, thereby perceiving oxygen tensions in environment indirectly

138 Symposium [S17] : Signal Transduction and Gene Regulation in Bacteria S17-4 Multiple Transcription Regulators of OhrR Family Responsible for Regulatory Cross Talk and Sequential Graded Gene Expression in Response to Organic Hydroperoxides in Agrobacterium tumefaciens Skorn Mongkolsuk 1,2 *, Nisanart Charoenlap 1,2, Surawach Rittiroongrad 1,2, Jintana Daungnkern 1, and Paiboon Vattanaviboon 1 1 Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, Thailand 2 Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand Analysis of A. tumefaciens genome reveals five OhrR family of transcription regulators are OhrR1, CpoR2, EstR3, CpoR4 and OhrR5. The expression of cpo1-mhpc were inducible by organic hydroperoxide treatment and relatively high uninduced levels in a cpor2 mutant. Further analysis of ability of different members of OhrR family to cross regulate cpo1-mhpc expression show high level expression of cpor2, cpor4 and OhrR5 repressed cpo1 promoter activity in organic hydroperoxide (ROOH) inducible manner except OhrR5. The basal cpo1 promoter activity was relatively high in cpor2 and cpor4 single mutants where its expression remained inducible by ROOH. This induction was abolished in cpor2-cpor4 double mutant. Taken the data together, there is regulatory cross talk between CpoR2 and CpoR4 at the cpo1 promoter. Examination of individual regulators of the OhrR family response to ROOH treatments revealed that OhrR responsed to 50 µm, CpoR2, CpoR4 OhrR5 responded to 250 µm and EstR responded to 500 µm cumene hydroperoxide (CuOOH) treatments. Thus, this allows A. tumefaciens differential graded response in that exposure to low concentration (50 µm) lead to expression of genes under OhrR controlled as concentrations increased to 500 µm genes under CpoR2, CpoR4, and OhrR5 would be additionally expressed. If the CuOOH continues to increase to 500 µm genes regulated by OhrR, CpoR2, CpoR4 OhrR5, EstR will be expressed

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140 Symposium [S18] Recent Advances in Deepsea and Extremophilic Microbiology

141 2016 International Meeting of the Microbiological Society of Korea S18-1 Microbial Diversity, Biotechnological Potential and Adaptation to Deep Sea Hydrothermal Vents Conditions Mohamed Jebbar Université de Bretagne Occidentale, CNRS, Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), 4 rue Dumont d Urville, Plouzané, France The deep biosphere (continental underground and in oceans below 1,000 m in depth) could represent up to 70% of all cells on Earth, as well as 50% of the primary production of biomass. The deep sea is characterized not only by high pressures (up to 110 MPa) but also by a lack of sunlight, an extremely low temperature (<5 C) except in the vicinity of hydrothermal vents, where temperature may be as high as 460 C, but water remains liquid owing to the high hydrostatic pressure (HHP). Recent trends and advances in molecular ecology, metagenomics, etc give evidence that microorganisms (Bacteria, Archaea, Viruses, Fungi, and Protists) represent by far the most important biological group on deep sea in terms of phylogenetic and functional diversity. The enormous diversity of deep sea microorganisms also gives rise to a largely untapped reservoir of genetic information, bioactive compounds and biomolecules (e.g. enzymes) which may find an application in various domains. Since the discovery of deep-sea hydrothermal vents, many mesophilic and hyper/thermophilic Bacteria and Archaea have been described but only a few thermo-piezophilic belonging mainly to the Thermococcales group have been described so far. The genome data mining showed no obvious general piezophilic signatures and the data related to genome expression (transcriptomic and proteomic studies) were performed by comparing piezophilic (Thermococcus barophilus, Pyrococcus yayanosii) versus non piezophilic (Thermococcus kodakarensis, Pyrococcus furiosus) species have highlighted the importance of several gene clusters and metabolic pathways, such as energy production and conversion, in the adaptation to HHP. Genetic manipulations were performed in T. barophilus in order to investigate the functions of some above pathways in vivo and to examine the roles of related enzymes. This will provide greater insight into the piezophilic lifestyle of thermo-piezophiles microorganisms dwelling in deep biosphere

142 Symposium [S18] : Recent Advances in Deepsea and Extremophilic Microbiology S18-2 Hydrogen Peroxide Detoxification: Physiological and Ecological Implications for Marine Ammonia-oxidizing Archaea Sung-Keun Rhee* and Jong-Geol Kim Department of Microbiology, Chungbuk National University Thaumarchaeota are abundant in marine environments and are known to catalyze ammonia oxidation in the oceans. Thus, the metabolism and physiology of these ammonia-oxidizing archaea (AOA) are of major significance for the global nitrogen cycle. However, fundamental nutritional principles related to cell growth and organic carbon assimilation by AOA are poorly understood. We isolated an ammoniaoxidizing archaeon (designated strain DDS1) from seawater and used this organism to study the physiology of ammonia oxidation. Strain DDS1 s ability to oxidize ammonia was enhanced in co-culture with other bacteria and in artificial seawater (ASW) medium supplemented with α-keto acids (e.g. pyruvate, oxaloacetate). An assay for heterotrophic growth indicated that organic carbon incorporation into archaeal cellular lipids was negligible. Lipid carbon atoms were, instead, derived from CO 2, indicating strict autotrophic growth. Further, chemicals that scavenge hydrogen peroxide (H 2 O 2 ), such as dimethylthiourea and catalase, replaced the α-keto-acid requirement for enhanced growth by strain DDS1. α-keto acids spontaneously detoxify H 2 O 2 via a nonenzymatic decarboxylation reaction. This decarboxylation mechanism was verified by showing that only the carboxyl carbon of pyruvate was released into the ASW medium. Strain DDS1 was shown in the absence of α-keto acids to endogenously produce H 2 O 2 (up to ca. 0.3 μm) which was inhibitory to growth. Genomic analyses indicate that all known AOA, including strain DDS1, lack putative catalase genes and are potentially sensitive to H 2 O 2. Our results show that strain DDS1 is a strict autotroph and implicate H 2 O 2 as a key factor determining the activity, evolution, and community ecology of AOA ecotypes

143 2016 International Meeting of the Microbiological Society of Korea S18-3 DNA Backbone Modification Expands Microbial Growth Range under Multiple Stresses Yan Yang #1, Guanpeng Xu #1, Jingdan Liang #1, Ying He 1, Lei Xiong 1, Hui Li 2, Douglas H. Bartlett 3, Zixin Deng 1, Zhijun Wang 1 *, and Xiang Xiao 1 * 1 State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, 2 Central Analytical Lab, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, P. R. China 3 Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, USA DNA phosphorothioate (PT) modification is a sulfur modification on the backbone of DNA introduced by the proteins Dnd A-E. It has been found within many bacteria ranging from soil-inhabiting, antibiotic-producing Streptomyces species to human pathogens 1-3. However, few studies have examined the physiological function of this modification. In this study, we observed that E. coli and Shewanella piezotolerance both expanded their growth ranges under multiple extreme conditions (such as extreme temperature, salinity, ph, pressure, UV, X-Ray) after their DNAs were phophorothioated by introduction of the dnd gene cluster. The phophorothioated DNA reacted to both hydroxyl radicals and H 2 O 2 in vivo, and protected genomic DNA as well as sensitive enzymes from intracellular oxidative damage. Considering that intracellular oxidative damage could result from multiple environmental stresses, the DNA PT modification certainly provides microorganisms with one type of survival advantage in nature. The findings in this study promote further exploration of PT modification usage, including possible applications in biological engineering and therapeutic treatments

144 Symposium [S18] : Recent Advances in Deepsea and Extremophilic Microbiology S18-4 Anaerobic Oxidation of Methane at the SMTZ of the Deep Sediments of Ulleung Basin, East Sea of Korea Jung-Hyun Lee 1 *, Jin-Woo Lee 1,2, and Kae Kyoung Kwon 1 1 Marine biotechnology Center, Korea Institute of Ocean Science & Technology 2 Department of Microbiology, Chungbuk National University We have shown that there was a discrete sulfate methane transition zone (SMTZ) in the methane hydrate-bearing sediment, Ulleung Basin, East Sea of Korea. Here, we characterized microbial and functional diversity of SMTZ responsible for anaerobic oxidation of methane (AOM) process in comparison with surface sediment, by combining 16S rrna gene amplicon pyrosequencing and metagenomic approach. The archaeal 16S rrna amplicon analysis showed that the Thermplasmata (59.3%), Marine Benthic Group B (MBGB; 14.2%) and anaerobic methanotroph-1b (ANME-1b; 10.2%) were dominated in SMTZ, whereas Thaumarchaeota (41.9%) and Miscellaneous Crenarchaeotal Group (MCG; 34.4%) were dominant taxa in the surface. In bacterial diversity, Chloroflexi (49.0%) and candidate division JS1 (24.4%) were dominated in SMTZ sediment. We obtained metagenomic sequences of 115 Mbp (476,661 reads with average sequence length of 242 bp) and 252 Mbp (701,377 reads with average sequence length of 359 bp), from SMTZ and surface sediments, respectively. Taxonomic profiling of the SMTZ metagenomics seqeunces showed that reads related with unclassified Dehalococcidetes and Methanosarcinales were overrepresented though methanogenesis related sequences were highly overrepresented in the metabolic profiling. The mapping analysis showed that most of sequences from the SMTZ metagenome were matched to ANME-1 draft genomes, rather than methanogens. Key genes necessary for methanogenesis were present in the SMTZ metagenome, except for N 5,N 10 -methenyltetrahydromethanopterin reductase (mer) and CoB-CoM heterodisulfide reductase subunits D and E (hdrde), which suggest that ANME-1b group is the primary player driving AOM process at the SMZ of methane hydrate-bearing sediments in the Ulleung Basin, East Sea of Korea

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146 Young Scientists' Forum

147 2016 International Meeting of the Microbiological Society of Korea YS1-1 Biocontrol of Staphylococcus aureus and Pectobacterium carotovorum by Bacteriocins and Application for Food Safety Jonguk Kim, Jisoo Hong, Jeong-A Lim, Jin-Woo Park, Jae-Gee Ryu, and Eunjung Roh* Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration * As the consumption of fresh-cut produce (ready-to-eat fruit and vegetables) has increased, problems were revealed. First, a certain amount of fresh-cut produce is deemed unusable by spoilage. Second, fresh-cut produce raise food safety concerns and a number of reports have referred to fresh vegetables harboring foodborne pathogens. Bacteriocins which are proteinaceous toxins produced by bacteria have drawn attention for their potential therapeutic applications in treating multi-drug resistant bacteria. Two bacteriocins were used for their biocontrol application. Carocin D inhibits growth of spoilage bacteria P. carotovorum. And the other bacteriocin produced by Staphylococcus pasteuri RSP-1 has been studied to control foodborne pathogen bacteria S. aureus. The purified bacteriocin of S. pasteuri RSP-1 is 5 kda. It is worth to improve as a biocontrol agent due to its heat and wide ph stability. The two bacteriocins reduced viable cell numbers of target bacteria by 3 to 6 log units compared to the untreated, respectively. Bacteriophages which are virus killing bacteria have examined in previous studies for biocontrol agent in food safety. Two bacteriophages of PP2 and SP6 which inhibit growth of P. carotovorum and S. aureus were used in this study. The mixture of bacteriocins with bacteriophages showed a synergistic effect on the inhibition of target bacteria and the antibacterial spectrum was extended. The results suggested that bacteriocins with bacteriophages will be useful candidates for biocontrol agents in the food industry. [This work was supported by a grant (PJ010921) from the Rural Development Administration, Republic of Korea.] KEYWORDS: bacteriocin, MRSA, spoilage, food safety 146

148 Young Scientists' Forum YS1-2 Source Tracking and Succession of Kimchi Lactic Acid Bacteria during Fermentation Se Hee Lee World Institute of Kimchi This study aimed at evaluating raw materials as potential lactic acid bacteria (LAB) sources for kimchi fermentation and investigating LAB successions during fermentation. The bacterial abundances and communities of five different sets of raw materials were investigated using plate-counting and pyrosequencing. LAB were found to be highly abundant in all garlic samples, suggesting that garlic may be a major LAB source for kimchi fermentation. LAB were observed in three and two out of five ginger and leek samples, respectively, indicating that they can also be potential important LAB sources. LAB were identified in only one cabbage sample with low abundance, suggesting that cabbage may not be an important LAB source. Bacterial successions during fermentation in the five kimchi samples were investigated by community analysis using pyrosequencing. LAB communities in initial kimchi were similar to the combined LAB communities of individual raw materials, suggesting that kimchi LAB were derived from their raw materials. LAB community analyses showed that species in the genera Leuconostoc, Lactobacillus, and Weissella were key players in kimchi fermentation, but their successions during fermentation varied with the species, indicating that members of the key genera may have different acid tolerance or growth competitiveness depending on their respective species

149 2016 International Meeting of the Microbiological Society of Korea YS1-3 Oxidative Stress Response in Acinetobacter oleivorans DR1 Jisun Kim and Woojun Park* Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University Bacterial cells exposed to endogenous or exogenous sources of oxidants have systems for detecting and removing them. The oxygen molecule can accept electrons from intracellular reductants and reactive oxygen species (ROS). ROS are deleterious species that react with various cellular components, thereby damaging them. In Escherichia coli, two redox-sensing proteins, SoxR and OxyR, are activated upon oxidation. These proteins regulate the expression of genes involved in oxidative stress defense. Researching the physiological and regulatory systems of oxidative stress response in Acinetobacter oleivorans DR1, will expand our knowledge on the basic mechanisms of stress responses in soil microbes. The diesel-degrading soil bacterium A. oleivorans DR1 contains OxyR homologue (AOLE_14380) and SoxR homologue (AOLE_12135). The oxyr mutant has increased hydrogen peroxide sensitivity compared the wild type. Two-dimensional gel electrophoresis was conducted to investigate the effect of hydrogen peroxide on whole protein expression. Among 13 up-regulated proteins, OxyR binding was confirmed at the promoter regions of ahpc, ahpf, trxb (thioredoxin-disulfide reductase), and oprc (outer membrane receptor protein), along with a putative catalase gene (AOLE_09800). Quantitative reverse transcriptase PCR (qrt-pcr) analysis of these genes was performed in the wild type and the oxyr mutant to identify the role of OxyR at the transcriptional level. Hierarchical expression and OxyR-binding of several OxyR-controlled genes suggested that concentration is an important factor in inducing the set of genes under H 2 O 2 stress. Reporter assay suggested that SoxR of A. oleivorans DR1 is functional and has differential sensitivity to different redox-active compounds (RACs) than do the SoxR proteins from E. coli and Pseudomonas putida. The effect of RACs pre-treatment showed a protective role of SoxR at high concentrations of RACs. To identify SoxR target genes in A. oleivorans DR1, we conducted RNA-sequencing analysis in the presence of RACs. After identifying differentially expressed genes and putative SoxR binding sites, a DNA binding assay with purified SoxR indicated that SoxR could bind to the promoter of sine (encoding a SoxR-induced endoribonuclease), argt (encoding a lysine-arginine-ornithine-binding periplasmic protein) and AOLE_18450, which encodes a putative membrane protein. Expression analysis using the constructed soxr mutant verified that these genes are under the control of SoxR on the transcriptional level. The sine gene was the only putative RNase-encoding gene that responded to RACs. Our data also showed that both SoxR and its target SinE have protective roles in cells in the presence of RACs and antibiotics. These findings are the first report on molecular mechanisms of two major redox sensors in Acinetobacter species

150 Young Scientists' Forum YS1-4 Expansion of Cultured Bacterial Diversity by Large-scale Dilution-to-Extinction Culturing from a Single Seawater Sample Seung-Jo Yang 1, Ilnam Kang 2, and Jang-Cheon Cho 2 * 1 Marine Microorganisms Team, National Marine Biodiversity Institute of Korea 2 Department of Biological Sciences, Inha University High-throughput cultivation (HTC) based on a dilution-to-extinction method has been applied broadly to the cultivation of marine bacterial groups, which has often led to the repeated isolation of abundant lineages such as SAR11 and oligotrophic marine gammaproteobacteria (OMG). In this study, to expand the phylogenetic diversity of HTC isolates, we performed a large-scale HTC with a single surface seawater sample collected from the East Sea, the Western Pacific Ocean. Phylogenetic analyses of the 16S rrna genes from 847 putative pure cultures demonstrated that some isolates were affiliated with not-yetcultured clades, including the OPB35 and Puniceicoccaceae marine group of Verrucomicrobia and PS1 of Alphaproteobacteria. In addition, numerous strains were obtained from abundant clades, such as SAR11, marine Roseobacter clade, OMG (e.g., SAR92 and OM60), OM43, and SAR116, thereby increasing the size of available culture resources for representative marine bacterial groups. Comparison between the composition of HTC isolates and the bacterial community structure of the seawater sample used for HTC showed that diverse marine bacterial groups exhibited various growth capabilities under our HTC conditions. The growth response of many bacterial groups, however, was clearly different from that observed with conventional plating methods, as exemplified by numerous isolates of the SAR11 clade and Verrucomicrobia. This study showed that a large number of novel bacterial strains could be obtained by an extensive HTC from even a small number of samples

151 2016 International Meeting of the Microbiological Society of Korea YS1-5 A Machine Learning Approach for Prediction of in situ Chlorinated Ethene Dechlorination Potential Jaejin Lee 1,2,3, Jeongdae Im 2,3, Ungtae Kim 4, and Frank E. Löffler 2,3,5,6 * 1 Division of Life Sciences, Korea Polar Research Institute 2 Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA 3 Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA 4 Department of Civil and Environmental Engineering, Cleveland State University, Cleveland, OH 44115, USA 5 Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996, USA 6 University of Tennessee and Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA Despite advances in physical or chemical remediation technologies, in situ bioremediation is required as a stand-alone or as part of a combined approach at chlorinated ethene-contaminated sites. Selecting the most suitable remedial strategy is often challenging due to uncertainties associated with the microbiology (e.g., presence and activity of Dehalococcoidesmccartyi[Dhc]) and geochemical factors influencing Dhc activity. Although extensive groundwater monitoring data have been collected for decades, the datarich-but-information-poor syndrome has not been resolved, because those datasets have not been systematically analyzed. In the present study, geochemical and microbial datasets collected from five contaminated sites were used to develop a predictive model using a machine learning (i.e., classification and regression tree (CART)) algorithm (i) rank the relative importance of parameters that affect in situ reductive dechlorination activity, and (ii) provide recommendations for selecting the optimalbioremediation approach at a specific site. The dataset was partitioned into a training set and a test set for model construction and performance evaluation, respectively. A10-fold cross-validation was conducted for 100 randomly rearranged datasets for coherence test and to select the best classification tree model. The representative CART model successfully predicted 3-month-ahead dechlorination potential with 76% and 70% accuracy for the training and the test set, respectively. Indirect indicators for low dissolved - oxygen (e.g., low NO 3 and NO - 2, high Fe 2+ and CH 4 ) were the most influential factors for predicting dechlorination potential, followed by total organic carbon content (TOC) and Dhccell abundance. Collectively, these findings indicate that machine learning-based data mining techniques applied to groundwater monitoring data can lead to the development of predictive decision-making models. A major need for improving the predictive capabilities and stability of the data mining approach is a curated, up-to-date and comprehensive collection of groundwater monitoring data

152 Young Scientists' Forum YS1-6 Isolation and Ecophysiological Characterization of a Polycyclic Aromatic Hydrocarbon-degrading Bacterium, Alteromonas naphthalenivorans SN2, from a Contaminated Tidal Flat Hyun Mi Jin 1,2 and Che Ok Jeon 1 * 1 Department of Life Science, Chung-Ang University 2 Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources Sea-tidal flats are characterized by high primary production and nutrient cycling rates, which may rely upon high microbial abundance and diversity. We hypothesized that the crude oil-contaminated Taean tidal flat might harbor diverse aromatic hydrocarbon-degrading microbial communities. The present study therefore aimed to investigate (i) the diversity and composition of the aromatic hydrocarcon (especially PAH)-degrading bacterial populations enriched from the crude oil-contaminated tidal flat on the Taean coast; (ii) the isolation of PAH biodegrading bacteria from the enriched bacterial consortia and their aromatic hydrocarbons degradation abilities; (iii) the characteristics of ecological survival strategy of isolates by metabolites and genomics analysis; (iv) the ecophysiological properties by genome-wide transcriptional analysis. This study showed that newly isolated PAH-degrading bacterium, Alteromonas naphthalenivorans SN2, has the potential to play prominent role in PAH metabolism in the crude oil-contaminated sediments and in seawater. A comprehensive analysis of the specific transcriptional cellular responses of strain SN2 under four environmental mimic conditions (tidal flat-naphthalene, tidal flat-pyruvate, seawater-naphthalene, and seawater-pyruvate) revealed strain SN2 to be an opportunistic marine r-strategist. We also documented the expression of certain ecological fitness traits--with strain SN2 showing an appreciable capacity to degrade pollutants, including PAH compounds, in seasonally cold tidal flat habitats. Moreover, these results present that strain SN2 shows promise for field application to the bioremediation of contaminated sea-tidal flats and seawater without the addition of nutrients

153 2016 International Meeting of the Microbiological Society of Korea YS2-1 Killing Vibrio cholerae by a Chemical Modulator for Glucose Metabolism Young Taek Oh 1, Hwa Young Kim 1,2, and Sang Sun Yoon 1,2 * 1 Department of Microbiology and Immunology, 2 Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine Vibrio cholerae, a Gram-negative bacterium, causes pandemic cholera. An oral rehydration solution (ORS), which contains a large amount of glucose, has been used as the primary treatment for cholera. Meanwhile, it has also been speculated that continuous administration of ORS may create glucose-enriched microenvironments in favor of V. cholerae growth. Previous studies revealed that the capability of V. cholerae 7th pandemic strain N16961 to produce acetoin is essential to maintain the survival fitness under glucose-rich environment. Production of acetoin, a neutral fermentation end-product, allows V. cholerae metabolizing glucose without ph drop, which is mediated by organic acid production. This notion also suggests that inhibition of acetoin fermentation may end up with killing V. cholerae by acidic ph stress under glucose-rich conditions. Here, we developed a simple high-throughput screen to identify inducers of metabolism-mediated acidification (termed as imac). Out of 8,364 compounds, we identified a chemical, 5-(4-chloro-2-nitrobenzoyl)-6-hydroxy-1,3-dimethylpyrimidine-2,4(1H,3H)-dione (imac17191) that killed glucose-metabolizing N16961 by ph drop. When N16961 was grown with extra glucose in the presence of 50 μm imac17191, acetoin production was completely suppressed and concomitant accumulation of lactate and acetate was observed. Beta-galactosidase activity assay using a single-copy P VC1589 ::lacz reporter fusion demonstrated that imac17191 likely inhibited acetoin production at the transcriptional level. Together, our results suggest that imac17191, acting as a metabolic switch, has a therapeutic potential to be developed as a novel antibacterial agent against cholera. KEYWORDS: Vibrio cholerae, glucose metabolism, acetoin, inducer of Metabolism-mediated ACidification (imac) 152

154 Young Scientists' Forum YS2-2 Improved Production of Immunosuppressant Rapamycin Young Ji Yoo and Yeo Joon Yoon* Division of Nano Science, Ewha Womans University Rapamycin is a 31-membered macrocyclic polyketide produced by Streptomyces rapamycinicus, possessing various biological and pharmacological activities including antifungal, immunosuppressive, antitumor, neuroprotective, and anti-aging activities. Because of its pharmacological importance and broad application, intense effort to enhance its yield and to understand its biosynthetic routes has been made during the past decades. In order to increase rapamycin titers, classical strain improvement methods relying on ultraviolet irradiation, chemical mutagenesis, and metabolic engineering technology, have been employed. Moreover, the combined strategies utilizing both metabolic engineering and classical random mutagenesis have also been applied for the improvement of rapamycin production. The biosynthesis of many secondary metabolites produced by Streptomyces species is known to be controlled by pathway-specific regulatory genes that are generally located within their biosynthetic gene clusters. Thus, manipulation of the RapY, and the RapR-RapS, pathway-specific regulatory genes can be adopted as an effective approach to improve the production levels of rapamycin. Accordingly, characterize the negative regulatory roles of RapY and the RapR RapS two-component system in the rapamycin biosynthesis of S. rapamycinicus through overexpression, in-frame deletion, complementation, and transcriptional analysis of the rapamycin biosynthetic genes in the wild-type and mutant strains. In addition, comparative transcriptional analysis of wild-type and deletion mutants revealed that RapY represses the expression of the ABC-transporter gene RapX, which plays a critical role in enhanced rapamycin production

155 2016 International Meeting of the Microbiological Society of Korea YS2-3 The Elongation Factor P Regulate Expression of Magnesium Transport Protein in Salmonella Typhimurium Eunna Choi, Yoontak Han, and Eun-Jin Lee* Department of Genetic Engineering, Colleges of Life Sciences and Graduate School of Biotechnology, Kyung Hee University The elongation factor P specifically functions to enhance translation of polyproline-containing proteins by alleviating the stalling of ribosomes at polyproline stretches. Many virulence factors generally contain polyproline stretches. Interestingly, the mgtcbr operon belong to magnesium transport encoded mgtb has been consecutive proline amino acid sequence is present in two places. We confirmed that second polyproline substitute to alanine mutant does not happen ribosome stalling without elongation factor P condition. Also, the second polyproline mutant elevated both mgtb transcription and translation regulation. We determined that this mutant s protein expression levels and replication efficiency was higher than wild-type inside macrophage. This fact reveals that mgtb polyproline mutant affected salmonella s virulence

156 Young Scientists' Forum YS2-4 The Effect of Rsd, the Anti-sigma Factor of σ 70, on Biofilm Formation and Motility in Escherichia coli Young-Ha Park 1, Mangyu Choe 1, Chang-Ro Lee 2, Si-Hyeon Um 3, Nam-Chul Ha 3, and Yeong-Jae Seok 1,4 * 1 Department of Biological Sciences and Institute of Microbiology, Seoul National University 2 Department of Biological Sciences, Myongji University 3 Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Research Institute for Agricultural and Life Sciences, Seoul National University 4 Department of Biophysics and Chemical Biology, Seoul National University In bacteria, σ subunit of RNA polymerase (RNAP) directs transcription initiation. The regulation of σ activity is important for fine tuning of gene expression. σ activity is determined by their cellular level, affinity for core RNAP, and interactions with regulatory proteins. In Escherichia coli, housekeeping σ factor, σ 70, has the highest affinity for core RNAP and is the most abundant σ factor. Rsd, regulator of sigma D, binds specifically to σ 70 and it has been known as an anti-σ factor of σ 70. Rsd inhibits transcriptional activity of σ 70 through the formation of Rsd-σ 70. This anti-σ 70 activity is regulated by the phosphorylation state-dependent interaction of Rsd with HPr, phosphocarrier protein which is a general component of the phosphoenolpyruvate: sugar phosphotransferase system (PTS). Recently, we determined the structure of the Rsd-HPr complex and revealed the binding site for HPr on the surface of Rsd partly overlaps with that for σ 70. Even though Rsd is known as an anti-σ 70 factor, no specific phenotype has been associated with deficiency or overexpression of Rsd to date. An rsd-deficient mutant shows no apparent differences in its growth and viability in various media, compared to wild type. In this study, we found new phenotypes of the rsd mutant. Deletion of rsd affected the cell surface hydrophobicity and also mutant cells sank much faster than wild type. In spite of its increased cell surface hydrophobicity, biofilm formation decreased in the rsd mutant. Through a transcriptomic analysis and a proteomic analysis, these phenotypes were resulted in the increased amount of outer membrane protein antigen 43 (Ag43) which is encoded by agn43 gene and its transcription is σ 70 -dependent. Ag43 is an autotransporter protein that promotes cell-to-cell aggregation by self-recognition. In vitro transcription and qrt-pcr analyses indicated that the expression of ang43 was inhibited by Rsd. Also, flagellin protein FliC decreased in the rsd mutant when we analyzed the outer membrane protein profiles. Despite the decreased level of FliC, and thereby decreased motility in the rsd mutant strain compared to wild-type strain, there was no significant difference in mrna level of flic. For this reason, we assumed that export of flagellin subunit could be inhibited by excess number of Ag43 at bacterial surface. Based on these results, we propose that Rsd decreases the transcriptional level of agn43 through the regulation of σ 70 activity and consequently it influences the biofilm formation and motility

157 2016 International Meeting of the Microbiological Society of Korea YS2-5 Role of Hepcidin in Salmonella Infection Jae-Ho Jeong 1, Don-kyu Kim 2, Hueng-Sik Choi 2, and Hyon E. Choy 1 * 1 Department of Microbiology, Chonnam National University Medical School 2 National Creative Research Initiatives Center for Nuclear Receptor Signals and Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University Iron plays important roles in both infection by microorganisms and host defense against the infection. In response to microbial infection, defensin-like peptide hepcidin limits the serum iron by reducing iron release from macrophages which is mediated by IL-6 signaling. In an attempt to elucidate the mechanism of iron regulation by hepcidin, we examined the role of nuclear receptor family members belonging to the NR3B subfamily in hepatocyte. Here, we report hepatic ERRγ gene expression was induced by Salmonella-stimulated interleuklin-6 (IL-6) signaling, and led to induction of hepcidin and hypoferremia in mice. Conversely, liver-specific ablation of ERRγ gene expression attenuated the Salmonella-mediated induction of hepcidin, and normalized the hypoferremia caused by Salmonella infection. An inverse agonist of ERRγ ameliorated Samonella-mediated hypoferremia through reduction of ERRγ-mediated hepcidin gene expression, and performed a potent antimicrobial function for the intracellular growth of Salmonella. Control of iron metabolism by an ERRγ-specific inverse agonist could be a novel therapeutic approach to host defense against intracellular bacteria

158 Young Scientists' Forum YS2-6 Rad53 Regulates Genotoxic DNA Damage Stress and Radiation Resistance through Mrr1 Transcription Factor in C. neoformans Kwang-Woo Jung 1, Dongho Kim 1, Sangyong Lim 1 *, and Yong-Sun Bahn 2 * 1 Research Division for Biotechnology, Korea Atomic Energy Research Institute 2 Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University The genome stability and integrity of cells are routinely confronted threat induced by both endogenous stress inducing DNA replication process and exogenous stresses including ionizing radiation and genotoxic DNA damage agents. Eukaryotic cells contain evolutionarily conserved DNA damage checkpoint pathways to counteract the effect arising from endogenous and exogenous DNA damage stress. Through analysis of the genome-wide gene deletion libraries of C. neoformans, we recently identified that components of the PI3K pathways including Mec1, Tel1, Rad53, and Chk1 are evolutionarily conserved and these components contribute to genotoxic DNA stress response. However, the downstream transcription factors of PI3K pathway in C. neoformans to regulate expression of DNA repair system are not identified and characterized. In this study, we found that Mrr1 (master regulator of radiation resistance) transcription factor was identified as downstream factor of Rad53 through transcriptome analysis and regulated expression levels of genes regarding to DNA repair system. Furthermore, the mrr1 mutant exhibited sensitivity in response to diverse DNA damage insults. Therefore, a unique transcription factor Mrr1 played critical roles in genotoxic DNA damage stress as well as radiation resistance in C. neoformans

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160 Graduates Students' Forum

161 2016 International Meeting of the Microbiological Society of Korea GS-1 Mycosphere of Tricholoma matsutake (Pine Mushroom): Its Impact and Role on the Microbial Community Seung-Yoon Oh and Young Woon Lim* School of Biological Sciences, Seoul National University Pine mushroom (Tricholoma matsutake) is an expensive forest product because of its specific flavor and uncultivable state. Moreover, pine mushroom has a symbiotic relationship with pine tree as ectomycorrhizal fungi. Besides its ecological and economic importance, pine mushroom provides a habitat for microbial community. The fairy ring and fruiting body of T. matsutake form a mycosphere where the environment is strongly affected by hyphal activity and has abundant nutrients. We have studied the microbial diversity and community in the mycosphere of T. matsutake using complementary approach based on culture-dependent and independent (NGS) methods. We found diverse bacteria and fungi in the fairy ring and fruiting body of T. matsutake, and some species are potentially novel species. In fairy ring of T. matsutake, microbial diversity was low and community structure was distinct compared to it of adjacent bulk soil. In the fruiting body of T. matsutake, we found that microbial diversity and community were different depend on parts of fruiting body. In addition, bacteria isolated from fruiting body of T. matsutake showed negative effect on growth of T. matsutake and fungi isolated from T. matsutake. Our results suggest that T. matsutake may select microbial community, and microbes may also actively use T. matsutake as a habitat. Therefore, the study of microbial community in the mycosphere of T. matsutake will help to understand the ecology of T. matsutake and fungi-bacteria interactions

162 Graduates Students' Forum GS-2 The Effect of Viscosity on Predation by Bdellovibrio bacterivorus HD100 Hansol Im School of Life Sciences, Ulsan National Institute of Science and Technology Bdellovibrio bacteriovorus is a predatory bacterium which lives by invading the periplasm of Gram-negative bacteria. Predation by B. bacteriovorus is reported as a new alternative treatment for multi-drug resistant pathogens. Therefore, evaluating the conditions of predation might determine the effective predation against pathogens. We found that viscosity of the media affect the predation patterns of B. bacteriovorus. Based upon confocal and bioluminescence analysis, it was indicated the velocity of the prey and predators would be contributed on these results. In the test, polyethylene glycol (PEG) and dextran (DEX) was used for artificial viscous media. Different concentration of those chemicals provides relationships of the viscosity and predation patterning. In high viscous media, their predation was prohibited. Oppositely, in lower concentration of those chemicals, the predation rates seem to be increased. It proved that there are physiological relationships between B. bacteriovorus velocity and their predation. Furthermore, those results would give the ecological significance of velocity on their predation patterns

163 2016 International Meeting of the Microbiological Society of Korea GS-3 Renewable Production of 1,3-Diaminoporpane (A Three Carbon Diamine) by Metabolically Engineered Escherichia coli Tong Un Chae 1, Won Jun Kim 1, Sol Choi 1, Si Jae Park 4, and Sang Yup Lee 1,2,3 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST 2 Bioinformatics Research Center, KAIST 3 BioProcess Engineering Research Center, KAIST 4 Department of Environmental Engineering and Energy, Myongji University Bio-based production of chemicals is important for sustainable chemical industry. Here, Escherichia coli is metabolically engineered to produce 1,3-diaminopropane (1,3-DAP), a monomer for polyamide. Comparison of heterologous C 4 and C 5 pathways for 1,3-DAP production by in silico flux analysis revealed that the C 4 pathway employing Acinetobacter baumannii dat and ddc genes, encoding 2-ketoglutarate 4-aminotransferase and L-2,4-diaminobutanoate decarboxylase, respectively, was more efficient. In a strain having feedback resistant aspartokinases, the ppc and aspc genes were overexpressed to increase flux towards 1,3-DAP synthesis. Also, knocking out pfka was found to increase 1,3-DAP production by applying 128 synthetic small RNAs. Overexpression of the ppc and aspc genes in the pfka deleted strain resulted in even higher production of 1,3-DAP. Fed-batch fermentation of the final engineered E. coli strain allowed production of 13 g/l of 1,3-DAP in a glucose minimal medium. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA M1A 2A ).] 162

164 Graduates Students' Forum GS-4 Identification and Regulatory Characteristics of Vibrio vulnificus plp Encoding a Phospholipase Essential for Pathogenesis Kyung Ku Jang, Zee-Won Lee, and Sang Ho Choi* National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research institute for Agriculture and Life Sciences, Seoul National University To identify Vibrio vulnificus genes induced by mucin, transcriptomes of V. vulnificus cells grown with mucin-containing media or exposed to the mucin-secreting HT-29 MTX cells were analyzed using RNA-seq. Among the genes induced by exposure to the mucin and the HT-29 MTX cells, a gene, annotated as a plp encoding a putative phospholipase Plp, was identified and further studied. Compared with the wild type, the plp mutant showed a low level of cytotoxicity toward the HT-29 MTX cells and reduced virulence in mice. The purified rplp protein exhibited phospholipase A 2 activity, indicating that Plp contributes to the lipolytic activity of the pathogen and thereby is essential for the pathogenesis. Examination of global regulatory proteins on the expression of plp revealed that HlyU and CRP upregulate the plp. The cellular levels of HlyU and CRP were not significantly affected by one another, indicating that the regulator proteins function cooperatively to activate plp rather than sequentially in a regulatory cascade. HlyU and CRP directly bind to the upstream of the plp promoter P plp. DNase I protection assays, together with the deletion analyses of P plp, demonstrated that HlyU binds to three specific sequences centered at -174, , and , and CRP binds specifically to the sequences centered at -68. Consequently, the combined results indicated that V. vulnificus plp encodes a phospholipase A 2 essential for virulence and is cooperatively regulated by HlyU and CRP

165 2016 International Meeting of the Microbiological Society of Korea GS-5 Salmonella Virulence Protein Activates Sugar Phosphate Uptake Jang-Woo Lee and Eun-Jin Lee* Microbial Genetics Lab, Department of Genetic engineering, College of Life Science, KyungHee University Salmonella enterica Typhimurium uhpt gene, encoding the sugar phosphate transport protein gene is regulated by three component system, response regulator, UhpA, sensor kinase, UphB and sugar phosphate sensor protein, UhpC. We found that Salmonella virulence protein induces expression of the uhpt gene in Salmonella not Escherichia coli. Salmonella virulence protein directly interacts with UphT. Salmonella virulence protein has three important residues, E84, N92 and C99 for interacts with other proteins. Among these mutants, Salmonella virulence proteinc99 can t interact with UhpT specifically

166 Graduates Students' Forum GS-6 The Ferrichrome Receptor A as a New Target for Pseudomonas aeruginosa Virulence Management Keehoon Lee 1,2, Kang-Mu Lee 1, Junhyeok Go 1,2, Jae-Chan Ryu 2,3, Ji-Hwan Ryu 3, and Sang Sun Yoon 1,2,4 * 1 Department of Microbiology and Immunology, Yonsei University College of Medicine 2 Brain Korea PLUS Project for Medical Science 3 The Research Center for Human Natural Defense System, Yonsei University College of Medicine 4 Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine Pseudomonas aeruginosa is an opportunistic pathogen, known to develop robust biofilms. The biofilm formed by P. aeruginosa can lead to serious problems, including various biofilm infections and the development of resistance against multiple antibiotics. In addition to the antibiotics resistance, P. aeruginosa enhances biofilm development when many antibiotics are presented at sub-minimal inhibitory concentrations (MICs). We screened a transposon mutant library to identify genes that affect enhanced biofilm development under sub-mic antibiotic treatments. We identified a mutant that harboring a transposon insertion in the fiua gene, which encodes ferrichrome receptor A. Biofilm formation with sub-mic antibiotic treatments was inhibited when the fiua gene was deleted. Moreover, the ΔfiuA, a non-polar fiua deletion mutant, exhibited significantly decreased elastase production. In vivo virulence experiments using Caenorhabditis elegans revealed that the ΔfiuA-fed group exhibited increased survival compared to the PAO1-fed group. We also used a murine airway infection model and discovered that ΔfiuA expresses significantly less pathogenicity than its parental strain, PAO1. We hypothesized that fiua have pleotropic functions that affect P. aeruginosa biofilm development and virulence. Our data indicate that FiuA is related to P. aeruginosa virulence, but is not significantly related to P. aeruginosa growth. It is important because strategies for managing pathogen virulence without killing have the potential to reduce the frequency of emergence of new antibiotic-resistant bacterial strains. The targeting of FiuA could enable the attenuation of P. aeruginosa virulence and may be suitable for the development of a drug that specifically controls the virulence of a pathogen

167 2016 International Meeting of the Microbiological Society of Korea GS-7 Immunization of 13 Amino Acid Peptide Targeting Srr Proteins Provide a Broad Spectrum of Protections Against Group B Streptococcal Infections Shunmei Lin Department of Biotechnology, Korea Atomic Energy Research Institute Group B streptococcus (GBS) is a main cause of sepsis and meningitis in early infancy from puerperal as well as adults worldwide. Unfortunately, the current licensed GBS vaccine is still not available for playing against diverse serotypes. Furthermore, some safety concerns for the pregnant. GBS can express a surface serine-rich repeat (SRR)-glycoproteins which is an important virulent factor attributing for GBS pathogenesis. Previously, the strain lacking srr showed diminishing persistence in mouse model of GBS vaginal colonization and less fatal to GBS infected mice. Since GBS could express either Srr1 or Srr2 surface proteins, we designed two peptide vaccines targeting the critical epitopes of the binding domains in Srr proteins. Srr1 (Latch-1) and Srr2 (Latch-2) peptides were conjugated with bovine serum albumin (BSA), and were examined as a vaccine to evaluate efficacy against GBS serotype V and III respectively. In a range of immunization of Srr1-BSA, Srr1 specific antibody responses (IgG and IgM) were correlated with the protection of GBS expressing Srr1 challenge. In addition, Srr1-BSA conjugated vaccine could effectively activate CD4+ T cells which would provide the cell-mediated protection against GBS challenge. As well as, Srr2 gave the prominent protection against challenge of GBS expressing Srr2. Thereafter, our results presented here suggested that the conjugated peptides would permit an ideal vaccine candidate for broad serotypes of GBS in the future

168 Graduates Students' Forum GS-8 Cps35/Swd2 Mediates the Histone Crosstalk between H2B Ubiquitination and H3K4 Methylation Shinae Park and Jung-Shin Lee* Department of Molecular Bioscience, Kangwon National University In the process of transcription, chromatin structure should be regulated by several mechanism such as histone modification. H3K4 methylation via Set1 complex is a one of the major histone modification and requires H2B K123 monoubiquitination via Rad6/Bre1 complex. Like this, a modification of one residue can alter the ability of a second residue to be modified by its modifying enzymes and it is called Histone crosstalk H3K4 methylation depending on H2Bub1 is highly conserved from yeast to human. Past studies have shown that Cps35/Swd2 of Set1 complex is a critical protein for translating this histone crosstalk. But it is unclear how Cps35/Swd2 associate with chromatin and Set1 complex. To identify what brings Cps35/Swd2 to chromatin in H2Bub dependent manner, we performed affinity purification. Our data show that Cps35/Swd2 interacts with Rad6, when only Rad6 can give ubiquitin to its substrate, H2BK123. C-terminal domain of Cps35/Swd2 is very important part for this interaction. So, we can suggest Cps35/Swd2 regulates histone crosstalk between H2Bub1 and H3K4me3 through the interaction with Rad6. [This work is supported by [NRF-2013R1A1A ], [NRF-2015R1A4A ], and [NRF-2015R1 D1A1A ].] 167

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170 Poster A. Systematics B. Ecology and Environmental Microbiology C. Applied Microbiology D. Immunology and Microbial Pathogenesis E. Physiology and Biochemistry F. Genetics G. Biotechnology H. Others

171 2016 International Meeting of the Microbiological Society of Korea A001 Paenibacillus baekrokdamisoli sp., nov., Isolated from Soil of Crater Lake Keun Chul Lee 1, Kwang Kyu Kim 1, Jong-Shik Kim 2, Dae-Shin Kim 3, Suk-Hyung Ko 3, Seung-Hoon Yang 3, and Jung-Sook Lee 1,4 * 1 KCTC/KRIBB, 2 GIMB, 3 World Heritage and Mt. Hallasan Research Institute, 4 UST A002 Rhodanobacter aciditrophus sp. nov., an Acidophilic Bacterium Isolated from Mine Wastewater Hyeon-Woo Koh, Sudas Rani, and Soo-Je Park* Department of Biology, Jeju National University A007 Flavihumibacter sediminis sp. nov., Isolated from Tidal Flat Sediment Do-Hoon Lee and Chang-Jun Cha* Department of Systems Biotechnology, Chung-Ang University A008 Taxonomic Study of the Genus Hymenobacter Joo Won Kang, Mi Sun Kim, Ji Hee Lee, Seon Choi, Da Hyun Kim, and Chi Nam Seong* Department of Biology, College of Life Science and Natural Resources, Sunchon National University A003 Lentibacillus kimchii sp. nov., an Extremely Halophilic Bacterium Isolated from Kimchi Young Joon Oh 1, Hae-Won Lee 2, Seul Ki Lim 1, Min-Sung Kwon 1, Jieun Lee 1, Ja-Young Jang 1, Jong Hee Lee 1, Hae Woong Park 3, Seong Woon Roh 4, and Hak-Jong Choi 1 * 1 Microbiology and Functionality Research Group, World Institute of Kimchi 2 Hygienic Safety and Analysis Center, World Institute of Kimchi 3 Advanced Process Technology Research Group, World Institute of Kimchi 4 Biological Disaster Analysis Group, Korea Basic Science Institute A004 Study on the Mycelium and Morphological Characteristic of Naematoloma sublateritium Jongwoon Choi, Hasun Kim, Dongyong Shin, and Yunkyeong Lee* Forest Research Institute of Gangwon-do A005 A Multiplex-PCR for Rapid Identification of 4 Enterococcus Species Using Species-Specific Primers from Comparative Genomics Jongbin Park 1, Gwi-Deuk Jin 2, Yong Hyun Kim 2, Jae In Pak 2,3, and Eun Bae Kim 2,3 * 1 Department of Animal Life System, College of Animal Life Sciences, Kangwon National University, 2 Department of Animal Life Science, College of Animal Life Sciences, Kangwon National University, 3 Division of Applied Animal Science, College of Animal Life Sciences, Kangwon National University A006 Investigating the Inflammatory and Phenotypic Responses of Multiple Cultured Human Epithelial Cells to Predatory Bacteria Wasimul Bari and Ajay K. Monnappa* Ulsan National Institute of Science and Technology A009 OrthoANI: An Improved Algorithm and Software for Calculating Average Nucleotide Identity Imchang Lee 1,2, Yeong Ouk Kim 2,3, Sang-Cheol Park 2,3, and Jongsik Chun 1,2,3 * 1 School of Biological Sciences, Seoul National University 2 Institute of Molecular Biology & Genetics, Seoul National University 3 Interdisciplinary Program in Bioinformatics, Seoul National University A010 A Bacterium Representing Novel Species in the Genus Sphingomonas, Isolated from Freshwater of Juam Reservoir Ji Hee Lee, Dae In Kim, Yong Seob Joo, Seo Young Kim, and Chi Nam Seong* Department of Biology, College of Life Science and Natural Resources, Sunchon National University A011 Aquimarina sp. nov., Isolated from Marine Sponge Dysidea sp. Ga Eun Lee and Jin Sook Park* Department of Biological Science and Biotechnology, Hannam University A012 Acetobacter oryzifermentans sp. nov., Isolated from a Korea Traditional Vinegar Ga Youn Cho and Che Ok Jeon* Department of Life Science, Chung-Ang University 170

172 Poster A013 Lapsobacter soli gen. nov., sp. nov., Isolated from Soil of a White Heron Nesting Site Min-Kyeong Kim 1, Yujin Choi 1, Tae-Su Kim 1,2, Ji-Hye Han 1,3, Yochan Joung 1,4, and Seung Bum Kim 1 * 1 Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 2 Clinical Drug Manufacturing Center, Osong Medical Innovation Foundation, 3 Bacterial Resources Research Team, Freshwater Bioresources Research Division, Nakdonggang National Institute of Biological Resources, 4 Department of Biology, Inha University A014 Flavobacterium keumense sp. nov., Isolated from Freshwater Adaeze Ekwe, Joong-hyeon Ahn, and Seung Bum Kim* Department of Microbiology and Molecular Biology, Chungnam National University A015 Thalassotalea litorea sp. nov., Isolated from Seashore Sand Heeyoung Kang, Haneul Kim, and Kiseong Joh* Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies A016 Roseovarius salarius sp. nov., Isolated from a Solar Saltern Haneul Kim 1, Heeyoung Kang 1, Yochan Joung 2, and Kiseong Joh 1 * 1 Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, 2 Department of Biological Sciences, Inha University A017 Zeaxanthinibacter aestuarii sp. nov., Isolated from Estuary Sediment and Emended Description of the Genus Asker2007 Yun Hee Lee, Hye Im Jeong, Sang Eun Jeong and Che Ok Jeon* Department of Life Science, Chung-Ang University A018 A First Report of Pseudoalteromonas tetraodonis Isolated from Apostichopus japonicas Guts Hyunjun Choi, Jihoon Jo, and Chungoo Park* School of Biological Sciences and Technology, Chonnam National University A019 A New Record of Didymella pinodella Isolated from a Fruit of Red Pepper in Korea Tham Thi Duong and Hyang Burm Lee* Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University A020 Pseudahrensia todarodis sp. nov., a Novel Bacterium Isolated from the Gut of a Japanese Flying Squid Hyun Sik Kim, Pil Soo Kim, Dong-Wook Hyun, June-Young Lee, Woorim Kang, Na-Ri Shin, Tae Woong Whon, and Jin-Woo Bae* Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University A021 A Novel Microbulbifer-like Bacteria Isolated from the Gut of Purple Sea Urchin, Heliocidaris crassispina June-Young Lee 1,2, Pil Soo Kim 1,2, Dong-Wook Hyun 1,2, Hyun Sik Kim 1,2, Na-Ri Shin 1,2, Mi-Ja Jung 1,2, Ji-Hyun Yun 1,2, Min-Soo Kim 1,2, Tae Woong Whon 1,2, and Jin-Woo Bae 1,2 * 1 Department of Life and Nanopharmaceutical Sciences, 2 Department of Biology, Kyung Hee University A022 Flexivirga lutea sp. nov., an Actinobacterium Isolated from the Stool of a Crested Ibis Woorim Kang, Dong-Wook Hyun, Pil Soo Kim, Na-Ri Shin, Hyun Sik Kim, June-Young Lee, Euon Jung Tak, and Jin-Woo Bae* Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University A023 Lacibacter nakdongensis sp. nov., Isolated from Sediments of Nakdong River Ji-Hye Han, Kiwoon Baek, and Mi-Hwa Lee* Bacterial Resources Research Team, Freshwater Bioresources Research Division, Nakdonggang National Institute of Biological Resources (NNIBR) A026 Emticicia fontis sp. nov., Isolated from Freshwater Gi Gyun Nam, Yochan Joung, and Jang-Cheon Cho* Department of Biological Sciences, Inha University A027 Genomic Characteristics of Strain IMCC26207, a Non-colony-forming Actinobacterium, Isolated from an Oligotrophic Freshwater Lake Suhyun Kim, Ilnam Kang, and Jang-Cheon Cho* Department of Biological Sciences, Inha University 171

173 2016 International Meeting of the Microbiological Society of Korea A028 Isolation of Three New Flavobacteriaceae Strains, Their Genome Characteristics, and Proposal of the Name Aurantibacter yeongjongensis gen. nov., sp. nov Yeonjung Lim 1, Yochan Joung 1, Seung-Jo Yang 2, and Jang-Cheon Cho 1 * 1 Department of Biological Sciences, Inha University, 2 National Marine Biodiversity Institute of Korea A029 Flavobacterium inkyungensis sp. nov., Isolated from Freshwater of an Artificial Pond Miri Park, Yochan Joung, Gi Gyun Nam, and Jang-Cheon Cho* Department of Biological Sciences, Inha University A030 Spirosoma aerophilum sp. nov., Isolated from Air Sample Soo-Jin Kim, Jae-Hyung Ahn, Hang-Yeon Weon, Seung-Beom Hong, Soon-Ja Seok, Jeong-Seon Kim, and Soon-Wo Kwon* Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration A031 Lysobacter terricola sp. nov., Isolated from Greenhouse Soil Soo-Jin Kim, Jae-Hyung Ahn, Hang-Yeon Weon, Seung-Beom Hong, Soon-Ja Seok, Jeong-Seon Kim, and Soon-Wo Kwon* Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration A034 Genomic Characterization of Strain IMCC26134, a Freshwater Verrucomicrobial Strain Isolated from Lakewater Ahyoung Choi 1,2, Ilnam Kang 1, Suhyun Kim 1, and Jang-Cheon Cho 1 * 1 Department of Biological Sciences, Inha University, 2 Culture Techniques Research Team, Nakdonggang National Institute of Biological Resources A035 Oleiagrimonas sediminis sp. nov., a Marine Bacterium Isolated from Tidal Flat Sediment and Emended Description of the Genus Oleiagrimonas Fang et al and Oleiagrimonas soli Sung-Hyun Yang 1, Hyun-Seok Seo 1, Chi Nam Seong 2, and Kae Kyoung Kwon 1 * 1 Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, 2 Department of Biology, College of Life Science and Natural Resources, Sunchon National University A036 Perlucidibaca aquatica sp. nov. Isolated from Freshwater Kiwoon Baek, Ji-Hye Han, and Mi-Hwa Lee* Nakdonggang National Institute of Biological Resources A037 Palleronia salinaecis sp. nov., a Halophilic Species Isolated from Solar Saltern Suhk Hwan Park and Geon Hyoung Lee* Department of Biology, Kunsan National University A032 Martelella suaedae sp. nov. and Martelella limoniae sp. nov., Isolated from the Root of Halophytes Eu Jin Chung 1,2, Jung Moon Hwang 1,2, Kyung Hyun Kim 3, Che Ok Jeon 3, and Young Ryun Chung 1 * 1 Division of Applied Life Science (BK21 Plus), Plant Molecular Biology & Biotechnology, 2 Freshwater Bioresources Culture Research Division, Nakdonggang National Institute of Biological Resources, 3 Department of Life Science, Chung-Ang University A033 Lacinutrix chionocetis sp. nov., Isolated from Gut of a Red Snow Crab Hyangmi Kim 1, Sunjoo Park 2, Hyun-Woo Oh 3, Kyung Sook Bae 2, and Doo-Sang Park 2,4 * 1 Freshwater Bioresources Culture Research Division, Nakdonggang National Institute of Biological Resources, 2 Bicrobiological Resources Center, KRIBB 3 Core Facility Management Center, KRIBB, 4 Department of Green Chemistry and Environmental Biotechnology, UST A038 Analysis of Non-ribosomal Peptide Synthetase Gene Cluster for Tolaasin Biosynthesis DoWon Kang 1,2, JungHun Jeon 1,2, Chang-Won Lee 1,2, and Jae Won Kim 1,2 * 1 Division of Applied Life Science (BK21 Research), 2 Research Institute of Life Sciences, Gyeongsang National University A039 The Diagnostic Characteristics are Highly Homoplasious Used in Cladonia gracilis and Cladonia cornuta Jae Eun So 1,2, Soon Gyu Hong 1,2, and Ji Hee Kim 1 * 1 Division of Polar Life Sciences, Korea Polar Research Institute 2 Department of Polar Sciences, University of Science & Technology 172

174 Poster A040 Zygomycete Fungi from Animal Dung in Korea Thi Thuong Thuong Nguyen, Seo Hee Lee, Sarah Bae, Sun Jeong Jeon, and Hyang Burm Lee* Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University A041 Chthonobacter albigriseus gen. nov., sp. nov., Isolated from Grass-field Soil in Korea Do Hak Kim, Minsun Kim, Hansol Kim, Keunsoo Kang, and Tae-Young Ahn* Department of Microbiology, College of Natural Sciences, Dankook University A042 Characterization and Description of Novel Strain RP18 T, Isolated from the Forest Soil Yongseok Ko, Han a Cho, Seoyoun Koo, and Tae-young Ahn* Department of Microbiology, College of Natural Sciences, Dankook University A043 Seddomonas intestinalis gen. nov., sp. nov., Isolated from Human Faeces Boram Seo 1, Ju Eun Yoo 1, Yung Mi Lee 3, and GwangPyo Ko 1,2 * 1 Department of Environmental Health, Seoul National University, 2 Center for Human and Environmental Microbiome, Seoul National University, 3 Division of Polar Life Sciences, Korea Polar Research Institute 173

175 2016 International Meeting of the Microbiological Society of Korea B001 Occurrence of Viable, Red-pigmented Haloarchaea in the Plumage of Captive Flamingoes Seong Woon Roh and Jong-Soon Choi* Biological Disaster Analysis Group, Korea Basic Science Institute B002 Diversity and Distribution of Nitrite Oxidoreductase Alpha Subunit (nxra) Gene in the Marine Sediments Rani Sundas, Hyeon-Woo Koh, and Soo-Je Park* Department of Biology, Jeju National University B003 Comparative Analysis of Microbial Communities and Soil Organic Carbon Utilization Associated with the Depth and Thawing Effects on Tundra Soil in Alaska Ha Ju Park 1, Hyun Park 1, Bang Yong Lee 2, Yoo Kyung Lee 2, and Dockyu Kim 1 * 1 Division of Life Sciences, 2 Arctic Research Center, Korea Polar Research Institute B004 Metagenomic and Functional Analyses of the Consequences of Reduction of Bacterial Diversity on Soil Functions and Bioremediation in Diesel-contaminated Microcosms Jaejoon Jung 1, Laurent Philippot 2, and Woojun Park 1 * 1 Department of Environmental Science and Ecological Engineering, Korea University 2 INRA Dijon, UMR 1347 Agroecologie, Dijon, France B005 Effect of Oil Contamination on the Resilience of Taxonomic and Functional Structure in the Korean Tidal Flat Jaejin Lee 1, Boram Kang 2, and Tae Kwon Lee 2 * 1 Division of Life Sciences, Korea Polar Research Institute 2 Department of Environmental Engineering, Yonsei University B006 Bacterial Diversity and Species Composition in Three Endemic Baikalian Sponges Eun-Young Seo 1, Dawoon Jung 1, Yochan Joung 2, and Tae Seok Ahn 1 * 1 Department of Environmental Science, Kangwon National University 2 Department of Biological Sciences, Inha University B007 Cecal Microbiome from Broiler Chickens Differing in Body Weight and Sex Kyu Chan Lee 1, Dong Yong Kil 2, and Woo Jun Sul 1 * 1 Department of Systems Biotechnology, Chung-Ang University 2 Department of Animal Science and Technology, Chung-Ang University B008 Structural Analysis of the Sensory Domain of the Aromatic-responsive Transcriptional Activator PoxR from Ralstonia eutropha Vinod Vikas Patil 1,2, Kwang-hyun Park 1,2, Seung Goo Lee 1,2, and Eui-Jeon Woo 1,2 * 1 Korea Research Institute of Bioscience and Biotechnology, 2 University of Science and Technology B009 Effect of Indigenous Microbiome in Soil on Salmonella enterica Survival Suin Yang, Sora Kim, Jeong-A Lim, Jin-Woo Park, Jae-Gee Ryu, and Kyu Seok Jung* Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration B010 Genomic Analysis of Confluentimicrobium naphthalenivorans NS6, a New Naphthalene Degrader HyeIm Jeong, Kyung Hyun kim, and Che Ok Jeon* Department of Life Science, Chung-Ang University B011 Chromobacterium piscinae and Predation by Bdellovibrio bacteriovorus HD100 Wonsic Mun, Seong Yeol Choi, and Robert J. Mitchell* School of Life Science, Ulsan National Institute of Science and Technology B012 Arbuscular Mycorrhizal Fungal Diversity in Post-mining Area and Natural Forest Area in Goesan, Korea Hyeok Park 1, Yu-Ra Bae 1, Dong-Yeo Kim 1, Kang-Hyun Ka 2, and Ahn-Heum Eom 1 * 1 Department of Biology Education, Korea National University of Education 2 Division of Wood Chemistry & Microbiology, Korea Forest Research Institute B013 Genome Analysis of Two Strains Belonging to the Genus Limnohabitans, a Major Bacterial Group in Keum River Joong-hyeon Ahn and Seung Bum Kim* Department of Microbiology and Molecular Biology, Chungnam National University B014 Effects of Urea on Abundance and Community of Nitrite-dependent Anaerobic Methane Oxidation Bacteria in a Rice Paddy Sang Eun Jeong, Hyo Jung Lee, and Che Ok Jeon* Department of Life Science, Chung-Ang University 174

176 Poster B015 Diversity of Bacteria Enriched with Coastal Area Samples Contaminated with Petroleum Oils Seon-Hee Kim and Hyung-Yeel Kahng* Department of Environmental Education, Sunchon National University B016 Isolation and Characterization of Calcium Carbonate Precipitating Bacillus and Sporosarcina Species from Concrete Hyun Jung Kim and Woojun Park* Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University B017 Genomic and Transcriptomic Analyses of Acinetobacter oleivorans DR1 under Long-chain Alkane Chulwoo Park, Jaejoon Jung, and Woojun Park* Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University B018 Comparative Genomics Reveals Insights into Adaptation of Ramlibacter Species to Different Soil Habitats Hyo Jung Lee and Che Ok Jeon* Department of Life Science, Chung-Ang University B019 Screening of Highly Efficient Nitrogen Fixing Bacteria from Soils and Their Utilization for Plant Growth Promotion Sun-hwan Jeong and Sang-Seob Lee* Department of Life Science, Kyonggi University B020 Screening and Isolation of BTEX Degrading Bacteria from Tongyeong Sea Water of Korea Hye Ji Kim and Sang-Seob Lee* Department of Life Science, College of Natural Science, Kyonggi University B021 Development of Antifungal Compounds from Streptomyces sp. NF186 against Fusarium oxysporum f. sp. conglutinans Sung-Jin Cho 1 and Sang-Seob Lee 2 * 1 Department of Biological engineering, Kyonggi University, 2 Department of Life Science, College of Natural Science, Kyonggi University B022 Comparative Analysis of Bacterial Communities Isolated from Polychaete Habitat and Non-habitat in a Coastal Wetland Microcosm Seyeon Shin and Hyung-Yeel Kahng* Department of Environmental Education, Sunchon National University B023 Gut Microbiota of Mottled Skates, Raja pulchra from the Yellow Sea Eun Bae Kim Department of Animal Life Science, College of Animal Life Sciences, Kangwon National University B024 Glyoxylate Bypass Governs Bacterial Respiration under Oxidative Stress Sungeun Ahn and Woojun Park* Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University B025 Endogenous Hydrogen Peroxide Increases Biofilm Formation by Inducing Exopolysaccharide Production in Acinetobacter oleivorans DR1 In-Ae Jang and Woojun Park* Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological, Engineering, Korea University B026 Fungistatic Activity of an α-aminophosphonate Chitosan Derivative against Aspergillus niger on Controlled Microgravity Yesupatham Sathishkumar 1, Kesavan Devarayan 2, Byoung-Suhk Kim 3, and Yang Soo Lee 4 * 1 Department of Forest Science and Technology, College of Agriculture and Life, 2 Department of Basic Sciences, Tamil Nadu Fisheries University, Nagapattinam, India, 3 Department of BIN Convergence Technology, Chonbuk National University, 4 Department of Forest Science and Technology, College of Agriculture and Life B027 A Study on the Biodegradation of Petroleum Oils by Marine Microbial Consortia Seon-Hee Kim and Hyung-Yeel Kahng* Department of Environmental Education, Sunchon National University 175

177 2016 International Meeting of the Microbiological Society of Korea B028 Spatial Disturbances in Altered Mucosal and Luminal Gut Viromes of Diet-induced Obese Mice Min-Soo Kim 1 and Jin-Woo Bae 2 * 1 Department of Biology, 2 Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University B029 Isolation and Functional Classification of Marine Bacteria in Jeju Coastal Areas Useful for Industrial Purposes Seyeon Shin 1, Dong-Heon Lee 2, and Hyung-Yeel Kahng 1 * 1 Department of Environmental Education, Sunchon National University 2 Research Institute for Basic Sciences, Jeju National University B030 Isolation of Brackish Water Bacterioplankton from Saemangeum by High-Throughput-Culturing Method Based on Cell-sorter Inoculation Hyoung Tae Cheon, Yeonjung Lim, Suhyun Kim, and Jang-Cheon Cho* Department of Biological Sciences, Inha University B031 Microbial Reduction of Nitrous Oxide under Environmentally Relevant Concentrations Doyoung Park and Sukhwan Yoon* Korea Advanced Institute of Science and Technology B032 A Soil Bacterial Strain Displays Rifampicin Resistance by Inactivation of the Antibiotic Ho-Jin Jang, Dae-Wi Kim, Do-Hoon Lee, and Chang-Jun Cha* Department of Systems Biotechnology, Chung-Ang University B033 Specific Fungal Endophyte Resources: Diversity, Characterization, and Comparative Analysis from Contrasting Coastal Environments of Korea Young-Hyun You 1 and Jong-Guk Kim 2 * 1 Marine Microorganism Team, National Marine Biodiversity Institute of Korea 2 School of Life Science, Kyungpook National University B034 Fish Possess Unique Microbial Communities Shaped by Surrounding Environments and Distinct from Other Vertebrate Pil Soo Kim 1, Jae Bong Lee 2, Min-Soo Kim 1, Tae Woong Whon 1, Dong-Wook Hyun 1, Ji-Hyun Yun 1, Na-Ri Shin 1, Mi-Ja Jung 1, and Jin-Woo Bae 1 * 1 Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, 2 National Fisheries Research & Development Institute B035 A Physiologically, Genetically, and Morphologically Novel Isolated Ammonia-oxidizing Archaeon, Nitrosocosmicus oleophilus, from Terrestrial Sediment Man-Young Jung 1, Heeji Hong 1, Eugene L. Madsen 2, Md Arafat Islam 1, and Sung-Keun Rhee 1 * 1 Department of Microbiology, Chungbuk National University 2 Department of Microbiology, Cornell University, Ithaca, New York, USA B036 The Effect of ph on Nitrogen Dissimilation of Shewanella loihica PV-4 and Its Implication in N 2O Emission and Nitrogen Retention Ha Yeon Kim and Suk Hwan Yoon* Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology B037 Physiological Properties of Bacterioplankton during Phaeocystis Bloom in Polynya of Amundsen Sea, Western Antarctica So-Jeong Kim 1, Jong-Geol Kim 1, Joo-Han Gwak 1, Hee-Ji Hong 1, Woon-Jong Yu 1, Md. Arafat Islam 1, Soo-Je Park 2, and Sung-Keun Rhee 1 * 1 Department of Microbiology, Chungbuk National University, 2 Department of Biology, Jeju National University B038 Bioprospecting for Amylase Producing Bacteria from Arctic Sea Samples Eungyeong Heo 1, Haju Park 2, Dockyu Kim 2, and Eungbin Kim 1 * 1 Department of Systems Biology, Yonsei University 2 Division of Life Sciences, Korea Polar Research Institute B039 Analysis of the Rhizosphere Microbiome of Tomato Cultivars that are Resistant or Susceptible to Bacterial Wilt Min-Jung Kwak 1, Su Yeon Choi 2, Ju Yeon Song 1, Hyun Gi Kong 2, Hyoung Ju Lee 2, Seon-Woo Lee 2, and Jihyun F. Kim 1 * 1 Department of Systems Biology, Yonsei University, 2 Department of Applied Biology, Dong-A University 176

178 Poster B040 Differential Compositions of Lichen Microbiomes in Cladonia gracilis According to the Positions at Thalli from King George Island, Antarctica Hyun-Ju Noh 1,2, Jang-Cheon Cho 2, and Soon Gyu Hong 1 * 1 Division of Polar Life Sciences, Korea Polar Research Institute, 2 Department of Biological Sciences, Inha University B046 Anthropogenic Effect on Prevalence of Antibiotic Resistance in Natural Environments Shalem Raj Padakandla 1, Dae-Wi Kim 2, Yejin Jang 1, Woo Jun Sul 2, Chang-Jun Cha 2, and Jong-Chan Chae 1 * 1 Division of Biotechnology, Chonbuk National University 2 Department of Systems Biotechnology, Chung-Ang University B041 Measuring Patterns by Geographical Locations in Marine Metagenome Data Using Newly Adopted Genotyping by Sequencing Hoon-Je Seong 1, Chung-Yeon Hwang 2, Hong-Hee Won 3, and Woo-Jun Sul 1 * 1 Department of Systems Biotechnology, Chung-Ang University 2 Division of Polar Biology and Ocean Sciences, Korea Polar Research Institute 3 Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA B042 Comparative Metagenomic Analysis of Microbial Communities in Wheat Nuruk Fermentation Jeong-Ah Seo 1, Minjoo Kim 1, Ki Young Yoon 2, Min-Jung Kwak 2, Jihyun F. Kim 2, and Ju Yeon Song 2 * 1 School of Systems Biomedical Science, Soongsil University 2 Department of Systems Biology, Yonsei University B043 Diversity and Enzyme Activity of Penicillium Species Associated with Macroalgae in Jeju Island Myung Soo Park 1, Seobihn Lee 1, Seung-Yoon Oh 1, Ga Youn Cho 2, and Young Woon Lim 1 * 1 School of Biological Sciences, Seoul National University 2 National Institute of Biological Resources, Environmental Research Complex B044 Genome Reconstruction for Prediction of Metabolic Potential of Subsurface Archaea in Intertidal Mud Flat Sediment Joo-Han Gwak 1, So-Jeong Kim 1, Woon-Jong Yu 1, Md. Arafat Islam 1, Soo-Je Park 2, and Sung-Keun Rhee 1 * 1 Department of Microbiology, Chungbuk National University 2 Department of Biology, Jeju National University B045 Antibiotics Resistant Testing of Vibrio and Oxytetracycline Resistant Bacteria Isolated from Fish Farming Water in Jeju Son G. Nguyen, Mincheol Kim, Jungman Kim, Nakwon Hwang, and Tatsuya Unno* Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University B047 Characterization of Ampicillin Resistant Aeromonas Species Isolated from Domestic Streams Shalem Raj Padakandla, Yejin Jang, and Jong-Chan Chae* Division of Biotechnology, Chonbuk National University B048 Genomic Insight of Bioplastic Production by Sphingobium chungbukense DJ77 T Motakatla Venkateswer Reddy 1, Young-Chang Kim 2, and Jong-Chan Chae 1 * 1 Division of Biotechnology, 2 Department of Microbiology, Chungbuk National University B049 Microbial Biogeography on the Sedimentary Environment Influenced by the Arctic Paleoclimate Dukki Han 1, Seung-Il Nam 2, and Hor-Gil Hur 1 * 1 School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 2 Korea Polar Research Institute B050 Identification of a Variant of New Delhi Metallo-βlactamase, NDM-9-Producing Klebsiella pneumoniae in an Urban River in South Korea Doris Y. W. Di 1, Jeonghwan Jang 2, and Hor-Gil Hur 1 * 1 School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 2 BioTechnology Institute, University of Minnesota, Saint Paul, MN 55108, USA B051 Genome Sequence Analysis of the Soil Microbe Dokdonella koreensis DS123 HyeonGwon Lee 1, Min-Jung Kwak 1, and Jihyun F. Kim 1,2 * 1 Department of Systems Biology and Division of Life Sciences, Yonsei University 2 Strategic Initiative for Microbiomes in Agriculture and Food, Yonsei University 177

179 2016 International Meeting of the Microbiological Society of Korea B052 Genome Sequence of Maribacter dokdonensis DSW-8, a Marine Bacterium Isolated from Seawater of Dokdo, an Island of the East Sea in Korea Jidam Lee 1, Min-Jung Kwak 1, Soon-Kyeong Kwon 1, and Jihyun F. Kim 1,2 * 1 Department of Systems Biology and Division of Life Sciences, Yonsei University, 2 Strategic Initiative for Microbiomes in Agriculture and Food, Yonsei University B053 Stimulation of Biomass and Lipid Productivity of Marine Diatom Chaetoceros Strains by Utilizing Mud and Food Waste Mixture Si Wouk Kim 1,2 *, Moon Jong Kim 1, and Geun Ho Gim 2 1 Department of Energy Convergence, Chosun University 2 Department of Environmental Engineering, Chosun University 178

180 Poster C001 3D Structure of Family 5 Extracellular Solute-binding Protein in Bifidobacterium longum KACC Junsang Ham, Han-ha Chai, Hyoun Wook Kim, Bu Min Kim, and Mi-Hwa Oh* National Institute of Animal Science, RDA C002 Genetic Strategies for Pikromycin Over-production in Streptomyces venezuelae Joon-Sun Choi, Ji-Eun Kim, and Jung-Hye Roe* School of Biological Sciences, College of Natural Science, Seoul National University C003 Secondary Metabolites for Plant Growth Promoting Produced by Rhodobacter capsulatus PS-2 Ki Moon Bong 1, Jong Min Kim 1, In Cheol Park 2, Chul Won Lee 3, and Pyoung Il Kim 1 * 1 Jeonnam Bioindustry Foundation, Bio Control Research Center 2 Agriculture Microbiology Division, National Academy of Agricultural Science 3 Department of Chemistry, Chonnam National University C004 Cultivation Conditions for Mass Production of an Antifungal Lipopeptide from Bacillus methylotrophicus GH1-13 Jong Min Kim 1, Ki Moon Bong 1, Gong Min Kim 1, JaeKyeong Song 2, Chul Won Lee 3, and Pyoung Il Kim 1 * 1 Jeonnam Bioindustry Foundation, Bio Control Research Center 2 Agriculture Microbiology Division, National Academy of Agricultural Science 3 Department of Chemistry, Chonnam National University C005 Multi-drug Resistant Staphylococcus aureus Growth Inhibition by Violacein Produced by Pseudoduganella Natural Isolated Strain NI28 SooYeon Kim, SeongYeol Chio, and Robert J. Mitchell* Ulsan National Institute of Science and Technology C006 Impact of Silicate Solubilizing Burkholderia ebumea CS4-2 on Rice Growth Sang-Mo Kang, Raheem Shahzad, Ko-Eun Lee, Yeon-Gyeong Park, Ah-Yeong Kim, Chang-Woo Seo, Sajjad Asaf, and In-Jung Lee* School of Applied Biosciences, Kyungpook National University C007 Growth Inhibition of Multidrug Resistant Staphylococcus aureus Using Cotton Fabric with Violacein Derivative SeongYeol Choi 1, HeeUn Kwon 1, SooYeon Kim 1, YeongMi Kwon 2, ChangSeok Lee 2, JinHyeng Lee 3, and Robert J. Mitchell 1 * 1 School of Life Science, Ulsan National Institute of Science and Technology 2 YeeJoo Research Institute, YeeJoo Corporation 3 Korea Institute of Ceramic Engineering and Technology C008 Isolation of Lipase Genes from Goat Ruminal Metagenomic Libraries Mi-Ra Kwon 1, Keun-Sung Kim 2, Jin-Sung Lee 3, Mi-Rim Park 1, Haesu Ko 1, and Kyung-Tai Lee 1 * 1 Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration 2 Department of Food Science and Technology, Chung-Ang University 3 Department of Biological Sciences, Kyonggi University C009 Functional Analysis Of Intracellular Nitric Oxide during Development in a Filamentous Fungus Anchalee Pengkit 1, Sung-Sil Jeon 2, Soo Ji Son 3, Jae Ho Shin 3, and Gyungsoon Park 1,2 * 1 Plasma Bioscience Research Center, Kwangwoon University 2 Department of Electrical and Biological Physics, Kwangwoon University 3 Department of Chemistry, Kwangwoon University C010 Identification of Protease Genes from Goat Ruminal Metagenomic Libraries Mi-Ra Kwon 1, Kyung-Tai Lee 1, Keun-Sung Kim 2, Jin-Sung Lee 3, Mi-Rim Park 1, and Haesu Ko 1 * 1 Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, 2 Department of Food Science and Technology, Chung-Ang University, 3 Department of Biological Sciences, Kyonggi University C011 Effect of Structure and Molecular Weight on the Permeabilizing Ability of Polyethyleneimine (PEI) Soh M. Sandrine and Robert J. Mitchell* Ulsan National Institute of Science and Technology C012 Characterization of Lactobacillus salivarius Strain Isolated from Piglet Feces for Probiotic Uses Gwi-Deuk Jin 1, Jongbin Park 2, and Eun Bae Kim 1 * 1 Department of Animal Life Sciences, Kangwon National University, 2 Department of Animal Life System, College of Animal Life Sciences, Kangwon National University 179

181 2016 International Meeting of the Microbiological Society of Korea C013 Single-stranded DNA Aptamers Targeting Antimicrobial Peptides: PG1, PR26 and PMAP36 Phat-Loc Nguyen 1, Kyeoung-Ah Lee 1, Simranjeet Singh Sekhon 1, Jiho Min 2, and Yang-Hoon Kim 1 * 1 Department of Microbiology, College of Natural Sciences, 2 Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University C014 Selection and Characterization of the Glypican-3 Binding DNA Aptamers Quang-Thai Nguyen 1, Kyeong-Ah Lee 1, Sinranjeet Singh Sekhon 1, Yang-Hoon Kim 1, Sung-Jin Cho 2, and Jiho Min 3 * 1 Department of Microbiology, 2 Department of Biology, 3 Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University C015 Deoxyviolacein Mass Production and Aggregation Using Fermenter with Escherichia coli Culture HeeUn Kwon, SeongYeol Choi, and Robert James Mitchell* School of Biological Science, Ulsan National Institute of Science and Technology C019 Anti-tumor Effect of L-asparaginase Delivered by Salmonella typhimurium on Solid Tumors Kwangsoo Kim, Daejin Lim, Yeongjin Hong, Kun-Hee Kim, Kyeong il Park, Shinnam Li, Hyun-Ju Kim, Hyon E. Choy, and Jae Ho Jeong* Department of Microbiology, Chonnam National University Medical School C020 Proteobacteria: Diagnostic Marker for Dysbiosis in Gut Microbiota Na-Ri Shin, Tae Woong Whon, and Jin-Woo Bae* Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University C021 Screening and Fermentation Characteristics of Bacillus sp. with High Amylase and Protease Activity Isolated from Traditional Nuruks Min Ju Park, Sung Wook Han, Su Jin Heo, Young Ho Hong, Seong Jun Cho, and Seung Won Park* Life Ingredient & Material Research Institute, CJ Cheil Jedang C016 Adaptive Laboratory Evolution of Leuconostoc mesenteroides J18 in Response to Low Temperature Hye Rim Kim 1, Ga Yeon Jo 2, Hey Hee Jeon 2, and Che Ok Jeon 2 * 1 Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Center for Food and Bioconvergence, Seoul National University 2 Department of Life Science, Chung-Ang University C022 Effect of Metabolic Condition Medication and Probiotics Formulation on the Model Rats and Their Intestinal Microbiota Seokcheon Song 1, Joohyun Shin 2, Jaegu Seo 2, Myungjoon Jung 2, and Eungbin Kim 1 * 1 Department of Systems Biology, Yonsei University, 2 R&D Center, Cellbiotech, Co. Ltd. C017 Activation of Endophytic-plant Growth-promoting Bacteria (EPGPB) by Dielectric Barrier Discharge Plasma Treatment Sang hye Ji 1, Se Chul Chun 2, Eun Ha Choi 1, and Gyungsoon Park 1 * 1 Plasma Bioscience Research Center, Kwangwoon University, 2 Department of Bioresources and food science, College of Life and Environmental Sciences, Konkuk University C018 Inhibition Effect of Lactobacillus plantarum in Colon Cancer Anna Jeong, Sooyeon Song, and Sejong Oh* Division of Animal Science, Chonnam National University C023 Combination of Synthetic Genetic Circuitry and Microfluidics for Highly Sensitive Heavy Metal Ion Biosensors Hyun Ju Kim 1,2, Ji Won Lim 3, Haeyoung Jeong 1,4, Sang-Jae Lee 5, Dong-Woo Lee 6, Taesung Kim 3,7, and Sang Jun Lee 1,2 * 1 Biosystems & Bioengineering Program, University of Science and Technology (UST), 2 Microbiomics and Immunity Research Center, Korea Research Institute Bioscience & Biotechnology (KRIBB), 3 Department of Biomedical Engineering, Ulsan National Institute of Science & Technology (UNIST), 4 Superbacteria Research Center, KRIBB, 5 Major in Food Biotechnology, Silla University, 6 School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea, 7 Department of Mechanical Engineering, UNIST C024 Lactobacillus plantarum Suppressed ß-hexoaminidase, Histamine, and Expression of TNF-α and IL-4 in BPA-stimulated RBL-2H3 Cells Jin A Lim and Sejong Oh* Division of Animal Science, Chonnam National University of Korea 180

182 Poster C025 Total Protein Isolated from Lactobacillus plantarum Has a Protective Character from Cadmium Chloride-stimulated Raw Cells Miyoung Shin and Sejong Oh* Division of Animal Science, Chonnam National University C027 Determination of Antiviral Effects of Adenosine Analogues on Epstein-Barr Virus Infection Miyeon Cho, Seok-Won Jung, Soomin Lee, and Hyojeung Kang* College of Pharmacy and Institute of Microorganisms, Kyungpook National University C026 Nexus Role of Paracoccus denitrificans in Simultaneous Removal of Nitrate, Iron and Arsenic Sunhwa Park and Hor-Gil Hur* School of Environmental Science and Engineering, Gwangju Institute of Science and Technology 181

183 2016 International Meeting of the Microbiological Society of Korea D001 Effect of Sub Minimal Inhibitory Concentration Chlorhexidine on Binding Characteristics of Streptococci and Actinomycetes So Yeon Lee and Si Young Lee* Department of Oral Microbiology, Colleage of Dentistry, Gangneung-Wonju National University D002 Whole Genome-scale Transcriptomic Analysis of c-di-gmp Signaling in Enteropathogenic Escherichia coli Hyung Tae Lee, Dalmuri Han, June Bong Lee, Chong-Hae Hong, and Jang Won Yoon* College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University D003 Ferroportin Promote ROS Influx into Salmonella Containing Vesicle Resulting in Intramacrophage Bacterial Killing Daejin Lim, Hyun-Ju Kim, Jea-Ho Jeong, Kwangsoo Kim, Kun-Hee Kim, Kyeongil Park, Shinan Li, and Hyon E. Choy* Department of Microbiology, Chonnam National University Medical School D004 Inhibition of Varicella-zoster Virus Replication by the Extract from Elaeocarpus sylvestris Na-Eun Kim, So-Hee Bae, June-Eun Kim, and Yoon-Jae Song* Department of Life Science, Gachon University D005 The Quorum Sensing-dependent Factor(s) Can Modulate the Activity of Protease IV, a Major Virulence Factor of Pseudomonas aeruginosa Jungmin Oh 1,2,3, Soo-Kyung Kim 1,2,3, Xi-Hui Li 1,2,3, and Joon-Hee Lee 1,2,3 * 1 Lab of Microbiology, 2 College of Pharmacy, 3 Department of Pharmacy, Pusan National University D006 A Salmonella Virulence Protein Interacts with PhoR That Activates Pho-regulon Soomin Choi Microbial Genetic Laboratory, College of Life Science, Kyung Hee University D007 Eukaryotic Stress Response Gene ATF3 Provides Protection from Staphylococcus aureus and Listeria monocytogenes Infections Sung-Yoep Lee, Suhkneung Pyo, and Dong Kwon Rhee* School of Pharmacy, Sungkyunkwan University D008 Isolation of New Bacteriophages to Control Pathogenic Bacteria, Bacillus cereus Jeong-A Lim, Sojung Kim, Jonguk Kim, Jisoo Hong, Eunjung Roh, Kyu Seok Jung, Jae-Gee Ryu, and Jin-Woo Park* Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration D009 The Putative Fungicidal Molecules Show an Antifungal Effect on Candida albicans Virulence Through a Regulating the Mitochondrial Activity Young Kwang Park 1, Se Woong Kim 1, Hwang Suk Kim 2, Hee-Yoon Lee 2, and Joon Kim 1 * 1 Lab. of Biochemistry, Division of Life Sciences, Korea University, 2 Department of Chemistry, KAIST D010 Vacuoles are Essential for Morphogenesis and Virulence in Candida albicans Se Woong Kim, Young Kwang Park, Yoo Jin Joo, Yu Jin Chun, Ju Yeon Hwang, Je-Hyun Baek, and Joon Kim* Lab of Biochemistry, Division of Life Sciences, Korea University D011 Ohmyungsamycins, New Antimycobacterial Cyclic Peptides, Activate Autophagy via AMP-activated Protein Kinase-mediated Signaling Tae Sung Kim 1,2, Yern-Hyerk Shin 3, Hye-Mi Lee 1,2, Soohyun Um 3, Jin Kyung Kim 1,2, Dong-Chan Oh 3, and Eun-Kyeong Jo 1,2 * 1 Department of Microbiology, College of Medicine, Chungnam National University, 2 Infection Signaling Network Research Center, 3 Natural Products Research Institute, College of Pharmacy, Seoul National University D012 Antagonistics against Pathogenic Fusarium solani and Fusarium oxysporum by Novel Peptides from Bacillus amyloliquefaciens PT14 Hee Kyoung Kang 1 and Yoonkyung Park 1,2 * 1 Department of Biomedical Science, Chosun University, 2 Research Center for Proteineous Materials, Chosun University 182

184 Poster D013 The Impact of Serum Albumin on Predation by Bdellovibrio bacteriovorus HD100 Ga Young Cho, Hansol Im, Ajay Monnappa, and Robert Mitchell* Ulsan National Institute of Science and Technology D014 Transcriptomic Profiles from Bdellovibrio bacteriovorus HD100 during Predation on an Extended-Spectrum Beta-Lactamase (ESBL) Strain of Escherichia coli SooIn Choi 1, Mohammed Dwidar 2, and Robert J. Mitchell 1 * 1 School of Life Science, Ulsan National Institute of Science and Technology 2 Okinawa Institute of Science and Technology, Japan D015 Genome-wide Transcriptome Analysis of Sch9-dependent Thermotolerance Mechanism Reveals the Dual Functional Heat Shock Factor 1, Hsf1, in Cryptococcus neoformans Dong-Hoon Yang 1, Kwang-Woo Jung 1, Soohyun Bang 1, Jang-Won Lee 1, Min-Hee Song 1, Yeonseon Lee 1, Eunji Jeong 1, Anna Floyd 2, Richard Festa 3, Giuseppe Ianiri 4, Alex Idnurm 4, Dennis Thiele 3, Joseph Heitman 2, and Yong-Sun Bahn 1 * 1 Department of Biotechnology, Center for Fungal Pathogenesis, Yonsei University, 2 Departments of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham 27710, NC, USA, 3 Department of Pharmacology & Cancer Biology and Biochemistry, Medicine, and Phamacology and Cancer Biology, Duke University Medical Center, Durham 27710, NC, USA, 4 Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, MO 64110, USA D016 Unravelling of the Target of Rapamycin (TOR1) Kinase Signaling Pathway in Human Fungal Pathogen Cryptococcus neoformans Yee-Seul So 1, Giuseppe Ianiri 2, Alex Idnurm 2, Jae-Hyung Jin 3, and Yong-Sun Bahn 3 * 1 Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 2 Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, MO 64110, USA, 3 Department of Biotechnology, Center for Fungal Pathogenesis, Yonsei University D017 Kinome Webs Reveal Novel Pathogenicity Networks in Human Fungal Pathogen Cryptococcus neoformans Kyung-Tae Lee 1, Yee-Seul So 2, Dong-Hoon Yang 2, Kwang-Woo Jung 2, Jaeyoung Choi 3, Dong-Gi Lee 4, Hyojeong Kwon 2, Juyeong Jang 2, Li Li Wang 2, Soohyun Cha 2, Gena Lee Meyers 2, Joohyeon Hong 2, Soohyun Bang 2, Je-Hyun Ji 2, Goun Park 2, Hyo-Jeong Byun 2, Sung Woo Park 2, Young-Min Park 2, Gloria Adedoyin 5, Taeyup Kim 5, Anna K Averette 5, Jong-Soon Choi 4, Eunji Cheong 2, Yong-Hwan Lee 3, and Yong-Sun Bahn 2 * 1 Department of Biotechnology, Center for Fungal Pathogenesis, 2 Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 3 Department of Agricultural Biotechnology, Seoul National University, 4 Biological Disaster Analysis Group, Korea Basic Science Institute, 5 Department of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, USA D018 Detection and Growth Inhibition of Streptococcus mutans by Aptamers kyeong-ah Lee 1, Simranjeet Singh Sekhon 1, Gna Ahn 1, Ji-Young Ahn 1, Yang-Hoon Kim 1, Sung-Jin Cho 2, and Jiho Min 3 * 1 Department of Microbiology, 2 Department of Biology, 3 Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University D019 Production and Characterization of Sodium Hydroxide Induced Vibrio parahaemolyticus Ghosts as a Potential Vaccine Candidate Hyun Jung Park, Seongmi Ji, Nagarajan Vinod, Sung Oh, Jung Mo Koo, Han Byul Noh, Ki-Sung Lee, Sei Chang Kim, and Chang Won Choi* Department of Biology & Medicinal Science, Pai Chai University D020 Production and Characterization of Hydrochloric Acid Induced Listeria monocytogenes Ghosts (LMGs) as a Potential Vaccine Candidate Seongmi Ji, Hyun Jung Park, Nagarajan Vinod, Sung Oh, Jung Mo Koo, Han Byul Noh, Ki-Sung Lee, Sei Chang Kim, and Chang Won Choi* Department of Biology & Medicinal Science, Pai Chai University D021 Identification and Mechanism of LL37 and Its Analogs with Potent Antimicrobial Activity against Acinetobacter baumannii Strains Eunji Park 1,2 and Yoonkyung Park 1,2 * 1 Research Center for Proteineous Materials (RCPM), 2 Department of Biotechnology and BK21-Plus Research Team for Bioactive Control Technology, Chosun University D022 Characterization of Antimicrobial Activity and Mechanism of Antimicrobial Peptide against Bacteria Su Jin Ko 1,2 and Yoonkyung Park 1,2 * 1 Research Center for Proteineous Materials (RCPM), 2 Department of Biotechnology and BK21-Plus Research Team for Bioactive Control Technology, Chosun University D023 Effect of HAMP and T1AMP on the Antimicrobial Activity against Pseudomonas aeruginosa and Multidrug-resistant Pseudomonas aeruginosa Min Kyung Kim 1,2 and Yoonkyung Park 1,2 * 1 Research Center for Proteineous Materials (RCPM), 2 Department of Biotechnology and BK21-Plus Research Team for Bioactive Control Technology, Chosun University 183

185 2016 International Meeting of the Microbiological Society of Korea D024 Antimicrobial Activity, Anti-biofilm and Action Mechanism of Charge-enriched AMPs against Both Gram-positive and Gram-negative Bacteria with Therapeutic Potential for Clinical Antibiotic-resistant Hyo Mi Han 1 and Yoonkyung Park 1,2 * 1 Research Center for Proteineous Materials (RCPM), 2 Department of Biotechnology and BK21-Plus Research Team for Bioactive Control Technology, Chosun University D025 Efficacy of Antimicrobial Peptide on Antibacterial, Anti-biofilm Activity Jong Gwan Park 1,2 and Yoon Kyung Park 2 * 1 Department of Convergence Science, Kongju National University 2 Research Center for Proteineous Materials (RCPM), Chosun University D030 Prevalence of Respiratory Viruses in Patients with Acute Respiratory Infections in Jeonbuk, 2015 Chan mun Jin 1, Yeun Jeong Kim 1, Keung Eu No 1, Seouk Hyeon Lim 1, Cheon Hyeon Kim 1, Hee Dong Jung 2, Hyang Min Cheong 2, Sung Soon Kim 2, and Ki Soon Kim 2 * 1 Jeollabukdo Institute of Health and Environment Research, 2 Center for Infectious diseases, KNIH, KCDC D032 Chitosan Nanoparticle Supplemented Diet Alters the Zebrafish Gut Microbiota Chathurica Udayangani, Sajith Dananjaya, Seung Beom Seo, and Mahanama De Zoysa* College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University D026 Antibacterial and Anti-inflammatory Activities of CMA3 Peptide with Low Cytotoxicity in Escherichia coli Jong-Kook Lee 1 and Yoonkyung Park 1,2 * 1 Research Center for Proteinaceous Materials (RCPM), 2 Department of Biotechnology and BK21 Research Team for Protein Activity Control, Chosun University D027 Antibacterial and Anti-inflammatory Effects of Novel P-AMP against Human Pathogens Na hee Kang 1,2 and Yoonkyung Park 1,2 * 1 Research Center for Proteineous Materials (RCPM), 2 Department of Biotechnology and BK21-Plus Research Team for Bioactive Control Technology, Chosun University D028 Immunization of Mice with Irradiated Whole Cell Vaccine Confers a Significant Degree of Protection against a Lethal Infection of Streptococcus agalactiae A-Yeung Jang, Yong Zhi, Bum Joo Kim, Zhao Lei, Zhang Jing, Shunmei Lin, and Ho Seong Seo* Radiation Biotechnology Division, Korea Atomic Energy Research Institute D029 Pathogenicity of Newly Isolated Human Ileal Streptococci Dong-Wook Hyun, Min-Soo Kim, Tae Woong Whon, Na-Ri Shin, Mi-Ja Jung, Pil Soo Kim, Hyun Sik Kim, June-Young Lee, Woorim Kang, and Jin-Woo Bae* Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University D033 Fusarium oxysporum Infestation of Zebrafish in Laboratory System Sang Yeop Shin, Chanuka Kulatunga, Dong Joon Kim, Bae Keun Park, and Mahanama De Zoysa* College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University D034 Antibiotic Resistance is Induced by a Bacterial Starvation Signal in Vibrio cholerae Hwa Young Kim, Young Taek Oh, and Sang Sun Yoon* Department of Microbiology and Immunology, Brain Korea 21 PLUS Project for Medical Science, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine D035 Structural Insight of Substrate Promiscuity for 2-deoxyribose-5-phosphate Aldolase (DERA) from Streptococcus suis Thinh-Phat Cao 1, Joong-Su Kim 2, and Sung Haeng Lee 1 * 1 Department of Cellular and Molecular Medicine, Chosun University School of Medicine, 2 Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology D036 Identification of Essential Genes of Pseudomonas aeruginosa for It Is Growth in Airway Mucus Mohammed Mohammed and Sang Sun Yoon* Department of Microbiology and Immunology, Brain Korea 21 PLUS Project for Medical Science, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine 184

186 Poster D037 Anti-tumor Effect of Quercetin in EBV-associated Human Gastric Carcinoma Hwan Hee Lee, Seulki Lee, Hyojeong Kang, and Hyosun Cho* Department of Pharmacy, Duksung Women s University D038 The Anti-HSV Effect of Quercetin in Raw Cells via Downregulation of Inflammatory Responses Seulki Lee, Hwan Hee Lee, and Hyosun Cho* Department of Pharmacy, Duksung Women s University D039 KSHV Infection of Primary Human Endothelial Cells Induces Complement Activation by Exosome-mediated Binding of Properdin Hyungtaek Jeon 1, Jisu Lee 1, Seung-min Yoo 1, Shou-Jiang Gao 2, and Myung-Shin Lee 1 * 1 Department of Microbiology and Immunology, Eulji University School of Medicine, 2 Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA D040 Significant Differences between Tick Bite Sites and Mite Bite Sites on Humans Choon-Mee Kim 1, Na-Ra Yoon 2, and Dong-Min Kim 2 * 1 Premedical Science, College of Medicine, Chosun University, 2 Department of Internal Medicine, College of Medicine, Chosun University D041 Utility of Tick-bite Site Samples for the Diagnosis of Human Granulocytic Anaplasmosis Choon-Mee Kim 1 and Dong-Min Kim 2 * 1 Premedical Science, College of Medicine, Chosun University, 2 Department of Internal Medicine, College of Medicine, Chosun University D042 Clinical Usefulness of Conventional PCR Targeting the 16S Ribosomal RNA for the Diagnosis of Scrub Typhus Choon-Mee Kim 1 and Dong-Min Kim 2 * 1 Premedical Science, College of Medicine, Chosun University, 2 Department of Internal Medicine, College of Medicine, Chosun University D043 Host Transcriptional Profiles of Acinetobacter baumannii-infected Human Lung Cancer Cell Sang-Yeop Lee 1, Edmond Changkyun Park 1,2, Sung Ho Yun 1, Chi Won Choi 1, Hayoung Lee 1, Gun-Hwa Kim 1,3, and Seung Il Kim 1 * 1 Division of Bioconvergence Analysis, Korea Basic Science Institute, 2 Department of Bio-Analytical Science, University of Science and Technology, 3 Department of Functional Genomics, University of Science and Technology D044 Conditionally Pathogenic Gut Microbes Promote Larval Growth by Increasing Redox-Dependent Fat Storage in High Sugar Diet-Fed Drosophila melanogaster Tae Woong Whon, Na-Ri Shin, Mi-Ja Jung, Dong-Wook Hyun, Hyun Sik Kim, Pil Soo Kim, and Jin-Woo Bae* Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University D045 Phylogenetic Analysis of Leptospira spp. in Wild Rodent at Gwangju Metropolitan Area, Republic of Korea 2014~2015 Jung Wook Park 1, Sun Hee Kim 1, Duck Woong Park 1, Hye Jung Park 1, So Hyang Jeong 1, Mi Hee Seo 1, Yong Seok Lee 1, Jae Keun Chung 1, Hyun Jae Song 2, Jung Yoon Lee 2, and Dong Min Kim 3 * 1 Health and Environment Institute of Gwangju, 2 Gwangju Health University 3 Chosun University, Medical College D046 Aggregatibacter actinomycetemcomitans Lipopolysaccharidemediated Induction of Chemokines MCP-1, MIP-1α, and IP-10 Occurs via Distinct Intracellular Signaling Pathways in Murine Macrophages Ok-Jin Park, Min-Kyung Cho, Cheol-Heui Yun, and Seung Hyun Han* Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University D047 Acquisition of Chemoresistance and Other Malignancy-related Features of Colorectal Cancer Cells are Incremented by Ribotoxic Mycotoxin and Antibiotics Chang-Kyu Oh 1, Dongwook Kim 2, Seung-Joon Lee 3, Seong-Hwan Park 3, and Yuseok Moon 3 * 1 Department of Biomedical Sciences, Pusan National University School of Medicine 2 National Institute of Animal Science, RDA 3 Department of Biomedical Sciences, Pusan National University 185

187 2016 International Meeting of the Microbiological Society of Korea D048 Acute Gastroenteritis Surveillance from Diarrheal Patients in Gwangju, Korea, During 2015 Seon Kyeong Kim, Hye-young Kee, Tae Sun Kim, Eun-hye Jo, Ji Hyun Shin, Dong Ryong Ha, Eun-Sun Kim, and Kye Won Seo* Health & Environment Institute of Gwangju D049 ɩnkt Cell Sensitization during Neonatal Respiratory Syncytial Virus Infection Induces Severe Pulmonary Pathology in Re-infected Adult Mice Seung Young Lee 1, Youran Noh 1, Semi Rho 1, Jung Hyun Goo 1, Min Jung Kim 1, Chang-Yuil Kang 2, Man Ki Song 1, and Jae-Ouk Kim 1 * 1 Laboratory Science, International Vaccine Institute 2 College of Pharmacy, Seoul National University D050 B Cell Infection by Kaposi s Sarcoma-associated Herpesvirus Jinjong Myoung Korea Zoonosis Research Institute, Chonbuk National University D051 OX40 and 4-1BB Differentially Inhibit KSHV Replication in B Cells and Endothelial Cells Jinjong Myoung Korea Zoonosis Research Institute, Chonbuk National University D052 Human Fucosyltransferase 2 Expression in Mouse Jinjong Myoung Korea Zoonosis Research Institute, Chonbuk National University D053 KSHV Infection in Established B Cells Require Cell-associated Viruses Jinjong Myoung Korea Zoonosis Research Institute, Chonbuk National University D054 Jak-STAT Pathway Enhances KSHV Replication in Endothelial Cells Jinjong Myoung Korea Zoonosis Research Institute, Chonbuk National University D055 Calcineurin Targets Involved in Stress Survival and Fungal Virulence Hee-Soo Park 1,2 *, Joseph Heitman 2, and Maria E. Cardenas 2 1 School of Food Science and Biotechnology, Kyungpook National University, 2 Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA D056 Prooxidant Activity of Pyrogallol on Vibrio vulnificus Infection Ju Young Lim and Young Ran Kim* College of Pharmacy, Chonnam National University D057 Role of Host Cell Filamin A in Vibrio vulnificus RtxA1 Toxin-induced Cytoskeletal Rearrangement and Cytotoxicity Ju Young Lim and Young Ran Kim* College of Pharmacy, Chonnam National University 186

188 Poster E001 Purification, Crystallization, and X-ray Crystallographic Studies on Bacillus stearothermophilus Molybdenum Cofactor-dependent YiiM Byeol Nam-gung and Sung-il Yoon* Department of Biomedical Convergence, College of Biomedical Science, Kangwon National University E002 Anthranilate Deteriorates Bacterial Biofilms Xi-Hui Li 1,2,3, Soo-Kyung Kim 1,2,3, Jungmin Oh 1,2,3, and Joon-Hee Lee 1,2,3 * 1 Lab of Microbiology, 2 College of Pharmacy, 3 Department of Pharmacy, Pusan National University E003 Ornithine Lipid-mediated Regulation of Virulence and Biofilm Formation in Pseudomonas aeruginosa Soo-Kyung Kim 1,2,3, Xi-Hui Li 1,2,3, and Joone-Hee Lee 1,2,3 * 1 Lab of Microbiology, 2 College of Pharmacy, 3 Department of Pharmacy, Pusan National University E004 Dissection of the HOG Pathway Activated by Hydrogen Peroxide in Saccharomyces cerevisiae Young Mi Lee 1,2, Eun Jung Kim 1,2, Ji Eun An 3, Ye Ji Lee 4, Eun Yong Choi 4, Won Ja Choi 2,3,4, Eun Pyo Moon 5, and Wan Kee Kim 1 * 1 Department of Pharmacology, School of Medicine, Ajou University, 2 Division of Ecological Sciences, College of Natural Sciences, 3 Department of Life Sciences and Division of Ecological Sciences, College of Natural Sciences, 4 Interdisciplinary Program of EcoCreative, College of Natural Sciences, Ewha Womans University, 5 Department of Life Sciences, College of Natural Sciences, Ajou University E007 Structural Analysis of Ligand Bound Complex of UdgX: A Unique Uracil DNA Glycosylase Superfamily Binding Uracil DNA Woo-Chan Ahn 1,2, Min-Ho Lee 1, Pau Biak Sang 3, Umesh Varshney 3, and Eui-Jeon Woo 1,4 * 1 Korea Research Institute of Bioscience and Biotechnology, 2 Department of Biological Sciences, KAIST Institute for the Biocentury, Korea Advanced Institute of Science and Technology, 3 Department of Microbiology & Cell Biology, IISc, Bangalore, India, 4 University of Science and Technology E008 Elucidation of Regulatory Genes for Enhancement of Rapamycin Production Jin A Jung, Eun ji Kim, Jae-yeon Hwang, Myoun Su Kim, Shi Ying Jin, Na Ryeong Lee, and Yeo Joon Yoon* Department of Chemistry and Nano Science, Ewha Womans University E009 Localization of an Acid Responsive Element in the Laccase Promotor Expressed at Low ph in Coprinellus congregatus Su Yeon Kim 1, Linh Trieu Dieu Nguyen 1,2, and Hyung Tae Choi 1 * 1 Molecular Microbiology Lab, Department of Biochemistry, Kangwon National University, 2 Vietnam E010 The Sugar-dependent Regulation of C-di-GMP and Signal Transduction System Kyoo Heo 1, Young-Ha Park 1, and Yeong-Jae Seok 1,2 * 1 Department of Biological Sciences and Institute of Microbiology, 2 Department of Biophysics and Chemical Biology, Seoul National University E005 Overexpression of OLE1 Enhances Stress Tolerance and Constitutively Activates the MAPK Hog1 through the MAPKKK Ssk2 Ye Ji Lee 1, Olviyani Nasutution 2, Young Mi Lee 3, Eun Jung Kim 3, Wan Kee Kim 3, and Won Ja Choi 1 * 1 Interdisciplinary Program of EcoCreative, 2 Division of Life and Pharmaceutical Sciences, Ewha Womans University, 3 Department of Pharmacology, School of Medicine, Ajou University E006 Structural and Functional Studies on Uracil DNA Glycosylase from Bradyrhizobium japonicum Vinod Vikas Patil 1,2, Ullas Valiya Chembazhi 3, Biak Sang Pau 3, Ravi Tiwari 4, Umesh Varshney 3, and Eui-Jeon Woo 1,2 * 1 Korea Research Institute of Bioscience and Biotechnology, 2 University of Science and Technology, 3 Department of Microbiology & Cell Biology, IISc, Bangalore, India, 4 School of Veterinary and Life Sciences, Murdoch University, Western Australia E011 Trans-4-hydroxy-l-proline: A Novel Constituent of Compatible Solute in Moderate Halophilic Bacteria Kyung Hyun Kim, Baolei Jia, and Che Ok Jeon* Department of Life Science, Chung-Ang University E012 Development of a Colorimetric Quantification Method for Characterization of Lactic Acid Bacteria Min Young Jung, Sung-Oh Sohn, Se Hee Lee, Boyeon Park, Hae Woong Park, and Jong-Hee Lee* World Institute of Kimchi 187

189 2016 International Meeting of the Microbiological Society of Korea E013 Investigation of the Interaction between HPr and FruR in Vibrio cholera Chang-Kyu Yoon 1, Hey-Min Kim 1, Young-Ha Park 1, Yeon-Ran Kim 1, and Yeong-Jae Seok 1,2 * 1 Department of Biological Sciences and Institute of Microbiology, 2 Department of Biophysics and Chemical Biology, Seoul National University E014 Generation of a FK506 Analogue through Genetic Engineering of Streptomyces Strain Xu Zhao, Hea Luying Shin, Heqing Cui, Ji Yoon Beom, Ji Young Lee, and Yeo Joon Yoon* Department of Chemistry and Nano Science, Ewha Womans University E015 OxyR-dependent Gene Expression Involved in Exopolysaccharide Production in Acinetobacter oleivorans DR1 Bora Shin and Woojun Park* Department of Environmental Science and Ecological Engineering, Korea university E016 The Structural Insights of a Cytosol Protein Disulfide Reductase DsbM from Pseudomonas aeruginosa Inseong Jo 1, In-Young Chung 2, You-Hee Cho 2, and Nam-Chul Ha 1 * 1 Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Research Institute for Agricultural and Life Sciences, Seoul National University 2 Department of Pharmacy, College of Pharmacy, CHA University E017 Cellular Responses of Hemolytic Bacillus cereus MH-2 Exposed to Epigallocatechin Gallate (EGCG) Dong-Min Kim, Sang-Kook Park, and Kye-Heon Oh* Department of Life Science and Technology, Soonchunhyang University E018 Isolation and Cellular Responses of Explosive HMX-degrading Bacterium and Its Morphological Changes Under Sublethal HMX Concentrations Dong-Min Kim, Sang-Kook Park, and Kye-Heon Oh* Department of Life Science and Technology, Soonchunhyang University E019 Physiological Activities of Two Wine Yeasts, Pichia Species Isolated from Crushed Grapes Sang-Kook Park, Dong-Min Kim, and Kye-Heon Oh* Department of Life Science and Technology, Soonchunhyang University E020 Karyopherins Involved in the Nuclear Actin Transport Immanuel Dhanasingh and Sung Haeng Lee* Department of Cellular and Molecular Medicine, Chosun University School of Medicine E021 Crystal Structure of Glycogen Branching Enzyme from Pyrococcus horikoshii Soo Hui Na and Nam Chul Ha* Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University E022 Crystal Structure of the Regulatory Domain of AphB, a Virulence Gene Activator from Vibrio vulnificus Nohra Park, Saemee Song, Inseong Jo, Sang Ho Choi*, and Nam-Chul Ha* Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University E023 Bacillus licheniformis Contains Two More PerR-like Proteins in Addition to PerR, Fur and Zur Orthologues Yoon Mo Yang, Jung Hoon Kim, Su Hyun Ryu, Yeh Eun Lee, and Jin Won Lee* Department of Life Science and Research Institute for Natural Sciences, Hanyang University E024 Effect of Exogenous Glutamine on Salmonella Replication under Nitrosative Stress Conditions Yoon Mee Park and Iel Soo Bang* Department of Microbiology and Immunology, Chosun University School of Dentistry 188

190 Poster E025 Crystal Structure of Bacterial 1-Cys Peroxiredoxin from Vibrio vulnificus and Its Structural and Functional Implications to Scavenging ROS and Nitric Oxide Jinsook Ahn 1, Kyung Ku Jang 1, Inseong Jo 1, Jin-Wook Yoo 2, Sang Ho Choi 1, and Nam-Chul Ha 1 * 1 Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Center for Food and Bioconvergence, Research Institute for Agricultural and Life Sciences, Seoul National University, 2 Department of Manufacturing Pharmacy, Pusan National University E026 The HPr-pyruvate Kinase Complex Protects Vibrio vulnificus Cells against H 2O 2 Stress Generated by Fungal Neighbors in the Presence of Glucose Hey-Min Kim 1, Young-Ha Park 1, Chang-Kyu Yoon 1, and Yeong-Jae Seok 1,2 * 1 Department of Biological Sciences and Institute of Microbiology, 2 Department of Biophysics and Chemical Biology, Seoul National University 189

191 2016 International Meeting of the Microbiological Society of Korea F001 Complete Genome Analysis of Vibrio parahaemolyticus FORC_023, Isolated from Storage Water for Raw Fish Han Young Chung, Byungho Lee, Eun Jung Na, and Sang Ho Choi* Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University F002 Complete Genome Sequence of Antibiotic and Anticancer Agent Violacein Producing Massilia sp. Strain NR 4-1 Nu Ri Myeong, Hoon Je Seong, Hye-Jin Kim, and Woo Jun Sul* Department of Systems Biotechnology, Chung-Ang University F003 Metabolic Role of MADS-box Transcription Factor Mbx2 in Schizosaccharomyces pombe Youngdae Seo and Jung-Hye Roe* School of Biological Sciences, College of Natural Science, Seoul National University F004 Role of Rsv1 in Responding to Glucose-starvation in Schizosaccharomyces pombe Eun-Jung Kim and Jung-Hye Roe* School of Biological Sciences, College of Natural Science, Seoul National University F005 A Genome-wide Transcriptomic Analysis of Radiation-responsive Genes in the Radiation-resistant Fungus, C. neoformans Kwang-Woo Jung 1, Min-Kyu Kim 1, Dongho Kim 1, Sangyong Lim 1, and Yong-Sun Bahn 2 * 1 Research Division for Biotechnology, Korea Atomic Energy Research Institute 2 Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University F006 Crystal Structure of Thermoplasma acidophilum XerA Recombinase Shows Large C-shape Clamp Conformation and cis-cleavage Mode for Nucleophilic Tyrosine Chang Hwa Jo 1, Junsoo Kim 1, Ah-reum Han 1, Sam Yong Park 2, Kwang Yeon Hwang 1, and Ki Hyun Nam 3 * 1 Division of Biotechnology, College of Life Sciences & Biotechnology, Korea University, 2 Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, Japan, 3 Pohang Accelerator Laboratory, Pohang University of Science and Technology F007 Oxidative Stress Response of Deinococcus geothermalis via a Cystine Importer Minwook Kim and Sung-Jae Lee* Department of Biology, Kyung Hee University F008 Complete Genome Sequence of Vibrio parahaemolyticus FORC_022, a Food-borne Pathogen from Soy Sauce Marinated Crab in South Korea Byungho Lee, Han Young Chung, Suyeon Kim, Eun Jung Na, and Sang Ho Choi* Foodborne-pathogens Omics Research Center, Department of Food Science and Biotechnology, Seoul National University F009 Transcriptome Analyses of a Novel Transcriptional Regulator HpxA Essential for Hypoxic Responses in Aspergillus nidulans Using RNA-Seq Sun-Ki Koh, Dawoon Chung, Jun-Yong Kwak, Mee-Hyang Jeon, and Suhn-Kee Chae* Department of Biochemistry, Paichai University F010 Yap1 and Skn7 are Involved in DNA Double-strand Break Repair by Homologous Recombination in Saccharomyces cerevisiae Myung Ju Kim 1,2, Dae Gwan Yi 3, Jihyun Lee 1,2, Ji Eun Choi 1,2, Bohyun Park 1, Sujin In 1, and Woo-Hyun Chung 1,2 * 1 College of Pharmacy, 2 Innovative Drug Center, Duksung Women's Universiity 3 Department of Biological Sciences, Seoul National University F011 Role of Conserved Residues within the Wedge Domain of Deinococcus radiodurans RecG Sun Wook Jeong, Jing Zhang, Lei Zhao, Min Kyu Kim, and Sangyong Lim* Research Division Biotechnology, Korea Atomic Energy Research Institute F012 Complete Genome Sequencing of Clinical Isolated Salmonella enterica FORC_020 and Comparative Genome Analysis with S. enterica Typhimurium LT2 and S. enterica Newport USDA-ARS-USMARC-1972 You-Tae Kim and Ju-Hoon Lee* Department of Food Science and Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University 190

192 Poster F013 ABC Transporter Atm1 Plays Roles in Mitochondrial Functions and Iron Homeostasis in Human Fungal Pathogen Cryptococcus neoformans Eunsoo Do, Se-Ho Park, and Won Hee Jung* Department of Systems Biotechnology, Chung-Ang University F018 Change of Biochemical Characteristics in aroa ompa Deletion in Salmonella enterica serovar Enteritidis Kiju Kim and Tae-Wook Hahn* College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University F014 Distinct Survival Strategies of Pseudomonas aeruginosa by Dual Promoters of the Major Catalase (KatA) In-Young Chung, Bi-o Kim, Hye-Jung Jang, and You-Hee Cho* Department of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University F019 Molecular Mechanism of Anti-repressor by Ler to LEE5 Promoter in Enteropathogenic Escherichia coli (EPEC) Minsang Shin 1 and Hyon E. Choy 2 * 1 Department of Microbiology, Kyungpook National University School of Medicine 2 Department of Microbiology, Chonnam National University Medical School F015 Functional Analysis of RraAS1 Interacting with the Catalytic Domain of RNase ES Daeyoung Kim, Sojin Seo, Boeun Lee, Minju Joo, Ji-Hyun Yeom, and Kangseok Lee* Department of Life Science, Chung-Ang University F020 Cloning and Expression of 5-aminolevulinic Acid Synthase Gene Involved in Secondary Metabolism of Kitasatospora cheerisanensis Hwang Jae Yoon and Doo Hyun Nam* College of Pharmacy, Yeungnam University F016 Functional Analysis of RraAS2, a Streptomyces coelicolor Homolog of RraA Jihune Heo, Sojin Seo, Boeun Lee, Daeyoung Kim, Minju Joo, Ji-Hyun Yeom, and Kangseok Lee* Department of Life Science, Chung-Ang University F017 RNase G Modulates Pathogenicity of Salmonella Typhimurium through the Control of hns mrna Abundance Hong-Man Kim 1, Daeyoung Kim 1, Minho Lee 1, Ji-Hyun Yeom 1, Boeun Lee 1, Wooseok Song 2, and Kangseok Lee 1 * 1 Department of Life Science, Chung-Ang University 2 Department of Microbiology, Catholic University of Daegu, School of Medicine F021 The Velvet Regulators and Their Targets in Aspergillus nidulans Hee-Soo Park 1,3 *, Pil Jae Maeng 2, and Jae-Hyuk Yu 3 1 School of Food Science and Biotechnology, Kyungpook National University, 2 Department of Microbiology and Molecular Biology, Chungnam National University, 3 Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA F022 Effects of the Number of the Origin of Replication on the Cell Physiology of Escherichia coli Hee Jin Yang, Yuna Jung, and Jihyun F. Kim* Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University 191

193 2016 International Meeting of the Microbiological Society of Korea G001 Metabolically Engineered Escherichia coli for Renewable Production of 3-Aminopropionic Acid Tong Un Chae 1, Chan Woo Song 1,2, and Sang Yup Lee 1,2,3 * 1 Department of Chemical and Biomolecular Engineering (BK21 Plus Program), KAIS, 2 BioProcess Engineering Research Center, KAIST, 3 BioInformatics Research Center, KAIST G002 Engineering TCA Cycle for Renewable Production of Fumaric Acid by Escherichia coli Tong Un Chae 1, Chan Woo Song 1,2, Dong In Kim 1,3, Sol Choi 1,2, Jae Won Jang 1,2, and Sang Yup Lee 1,2,3 * 1 Department of Chemical and Biomolecular Engineering (BK21 Plus Program), KAIST, 2 BioProcess Engineering Research Center, KAIST, 3 BioInformatics Research Center, KAIST G003 Microbial Production of Gamma-butyrolactone via Metabolically Engineered Mannheimia succiniciproducens Tong Un Chae 1, Sol Choi 1,2, and Sang Yup Lee 1,2,3 * 1 Department of Chemical and Biomolecular Engineering (BK21 Plus Program), KAIST, 2 BioProcess Engineering Research Center, KAIST, 3 BioInformatics Research Center, KAIST G004 Production of Tyrosine and Cadaverine by Metabolically Engineered Escherichia coli Using Synthetic Small Regulatory RNA Ji Yeon Ha 1, Dokyun Na 2, Seung Min Yoo 1, and Sang Yup Lee 1,3,4 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), KAIST, 2 School of Integrative Engineering, Chung-Ang University, 3 Bioinformatics Research Center, KAIST, 4 BioProcess Engineering Research Center, KAIST G005 Production of Phenol from Glucose by Metabolically Engineered Escherichia coli Ji Yeon Ha 1, Byoungjin Kim 1, Hyegwon Park 1, Dokyun Na 2, and Sang Yup Lee 1,3,4 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), KAIST, 2 School of Integrative Engineering, Chung-Ang University, 3 Bioinformatics Research Center, KAIST, 4 BioProcess Engineering Research Center, KAIST G006 Rapid Multiple Gene Knockout System Using Integration-helper Plasmid Ji Yeon Ha 1, Chan Woo Song 1, and Sang Yup Lee 1,2,3 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), KAIST, 2 Bioinformatics Research Center, KAIST, 3 BioProcess Engineering Research Center, KAIST G007 Native-sized Spider Silk Protein Production by Enhanced Glycine Pool Ji Yeon Ha 1, Xiao-Xia Xia 1, Do Kyun Na 2, and Sang Yup Lee 1,3,4 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), 2 School of Integrative Engineering, Chung-Ang University, 3 Bioinformatics Research Center, KAIST, 4 BioProcess Engineering Research Center, KAIST G008 Deletion of the Butyrate Kinase (buk) Gene is Essential for the High Butyric Acid Selectivity in Clostridium acetobutylicum Seon Young Park 1, Yu-Sin Jang 1, and Sang Yup Lee 1,2 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute, 2 BioProcess Engineering Research Center, Bioinformatics Research Center, Korea Advanced Institute of Science and Technololgy (KAIST) G009 Construction of Isopropanol-Butanol-Ethanol Production Platform in the Recombinant Clostridium Strains by Metabolic Engineering Seon Young Park 1, Joungmin Lee 1, Yu-Sin Jang 1, and Sang Yup Lee 1,2 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute, 2 BioProcess Engineering Research Center, Bioinformatics Research Center, Korea Advanced Institute of Science and Technololgy (KAIST) G010 Metabolic Engineering of Escherichia coli for Short Chain Alkanes Production Seon Young Park 1, Yong Jun Choi 1, and Sang Yup Lee 1,2,3 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, 2 Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, 3 Department of Bio and Brain Engineering and Bioinformatics Research Center G011 Production of 1-Propanol by Metabolically Engineered L-threonine Overproducing Escherichia coli Seon Young Park 1, Yong Jun Choi 1, Jin Hwan Park 1, Tae Yong Kim 1, and Sang Yup Lee 1,2,3 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, 2 Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, 3 Department of Bio and Brain Engineering and Bioinformatics Research Center 192

194 Poster G012 Production of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by Metabolically Engineered Escherichia coli Kyeong Rok Choi 1, Jung Eun Yang 1, Yong Jun Choi 2, Seung Hwan Lee 3, Bong Keun Song 4, Si Jae Park 5, and Sang Yup Lee 1 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus Program), BioProcess Engineering Research Center, KAIST, 2 School of Environmental Engineering, University of Seoul, 3 Dept. of Biotechnology and Bioengineering, Chonnam National University, 4 Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, 5 Department of Environmental Engineering and Energy, Myongji University G013 Amino Acid L-arginine Production in a Metabolically Engineered Corynebacterium glutamicum Kyeong Rok Choi 1, Seok Hyun Park 1, Hyun Uk Kim 1,2, Tae Yong Kim 1,2, Jun Seok Park 3, Suok-su Kim 3, and Sang Yup Lee 1,2,4 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus rogram), Center for Systems and Synthetic Biotechnology KAIST, 2 BioInformatics Research Center, KAIST, 3 Daesang Corporation Research Center, 4 BioProcess Engineering Research Center, KAIST G014 Cadaverine Overproduction in a Metabolically Engineered Escherichia coli Cell Factory Kyeong Rok Choi 1, Zhi-Gang Qian 1, Hye Min Park 1, and Sang Yup Lee 1,2,3 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus rogram), Center for Systems and Synthetic Biotechnology KAIST, 2 Institute for the BioCentury, KAIST, 3 BioProcess Engineering Research Center, KAIST G017 Construction of Acidic Amino Acids Sensing Escherichia coli by Introduction of Chimeric Two-component System Murali kannan Maruthamuthu and Soon Ho Hong* Department of Chemical Engineering, University of Ulsan G018 3D Wave of Fermented Soybean Extracts Suppress Proliferation of Human Breast Cancer MDA-MB-231 Cells Jameon Park 1,2 and Han Bok Kim 1,2 * 1 Department of Biotechnology, Hoseo University, 2 The Research Institute for Basic Sciences, Hoseo University G019 Isolation of Biogenic Amine Non-producing Bacillus subtilis SCM121 and Medium Optimization for Improving Biomass by Response Surface Methodology Su-Ji Jeong, Hee-Jong Yang, Seong-Yeop Jeong, Jeong Seon Eom, and Do-Youn Jeong* Microbial Institute for Fermentation Industry (MIFI) G020 Isolation of Bacillus subtilis SCM146 Having Antimicrobial Activity and Medium Optimization for Improving Biomass by Response Surface Methodology Hee-Jong Yang, Su-Ji Jeong, Seong-Yeop Jeong, Jeong Seon Eom, and Do-Youn Jeong* Microbial Institute for Fermentation Industry (MIFI) G015 L-Ornithine Bioproduction in Metabolically Engineered Corynebacterium glutamicum Kyeong Rok Choi 1, Seo Yun Kim 1, Joungmin Lee 1, and Sang Yup Lee 1,2,3 * 1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus rogram), Center for Systems and Synthetic Biotechnology KAIST, 2 BioInformatics Research Center, KAIST, 3 BioProcess Engineering Research Center, KAIST G016 Engineering of Metabolic Flux into Novel Gamma-aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds in Escherichia coli Van Dung Pham, Sivachandiran Somasundaram, and Soon Ho Hong* Department of Chemical Engineering, University of Ulsan G021 Isolation of Biogenic Amine Non-producing Lactobacillus brevis SCML67 and Medium Optimization for Improving Biomass Using Statistical Technique Su-Ji Jeong, Hee-Jong Yang, Seong-Yeop Jeong, Jeong Seon Eom, and Do-Youn Jeong* Microbial Institute for Fermentation Industry (MIFI) G022 Isolation of Biogenic Amine Non-producing Lactobacillus brevis SCML458 Having Antioxidant Activity and Medium Optimization for Improving Biomass Hee-Jong Yang, Su-Ji Jeong, Seong-Yeop Jeong, Jeong Seon Eom, and Do-Youn Jeong* Microbial Institute for Fermentation Industry (MIFI) 193

195 2016 International Meeting of the Microbiological Society of Korea G023 KCl Effect on Growth of Leuconostoc mesenteroides CS-5 under the High Salt Concentration Oh Sik Kwon Major in Biological Science, College of Natural Science, Keimyung University G024 Oxidation of Methane throuth Component Interactions from Type II Methanotrophs Min Young Song 1, Eun Taek Seol 1, and Seung Jae Lee 1,2 * 1 Department of Chemistry, Chonbuk National University 2 Research Institute of Physics and Chemistry, Chonbuk National University G025 Stability and Inactivation Mechanism of Porcine Parvovirus against High Temperature Treatment Sang Eun Han 1, Jung Eun Bae 2, and In Seop Kim 1 * 1 Department of Biological Sciences and Biotechnology, Hannam University 2 BioPS G026 Biofunctionality and Bioconversion Activities of KA111 (Pseudomonas fragi), KA115 (Bacillus methylotrophicu), KA182 (Bacillus subtilis), KA198 (Bacillus safensis), KA217 (Bacillus pumilus), KA222 (Bacillus licheniformis) Sung-Ho Joo, Won Mun Kim, Kwang-Su Lee, and Ki-Sung Lee* Department of Biology & Medicinal Sciences, Pai Chai University G027 Bioconversion Efficiency in Accordance with the Substrate Concentrations Sung-Ho Joo, Won Mun Kim, Kwang-Su Lee, and Ki-Sung Lee* Department of Biology & Medicinal Sciences, Pai Chai University G028 Characterization and Validation of the Modified tuf Promoter to Develop the Efficienct Recombinant Protein Expression Vector System for Lactococcus lactis IL1403 Inseon Kim 1,2, Jinduck Bok 2, Sangkee Kang 2, Chongsu Cho 1, and Yunjaie Choi 1 * 1 Department of Agricultural biotechnology, Seoul National University 2 Institute of Green-Bio Science & Technology, Seoul National University G029 Inhibition of Enterotoxigenic Escherichia coli Cell-growth by ETEC Specific Binding DNA Aptamer Woo-Ri Shin 1, Sang-Hee Lee 1, Ji-Young Ahn 1, Jiho Min 2, and Yang-Hoon Kim 1 * 1 Department of Microbiology, College of Natural Sciences, Chungbuk National University, 2 Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University G030 Human Immunodeficiency Virus Type 1 Safety of Blood Coagulation-related Plasma Products Jung Eun Bae, Eun Kyo Jeong, Dong Joo Yu, Da Jeong Kim, Jung Sun Jeong, Sang Eun Han, and In Seop Kim* Department of Biological Sciences and Biotechnology and Center for Biopharmaceuticals Safety Validation, Hannam University G031 CTHRC1 Protein Binding RNA-aptamer Selection by SELEX Hee-Young Cho 1, Woo-Ri Shin 1, Kyeong-Ah Lee 1, Se Hee Lee 1, Simranjeet Singh Sekhon 1, Ji-Young Ahn 1, Dae-Ghon Kim 2, Jiho Min 3, and Yang-Hoon Kim 1 * 1 Department of Microbiology, College of Natural Sciences, Chungbuk National University, 2 Research Institute of Clinical Medicine of Chonbuk National University, 3 Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University G032 Isolation and Identification of Strain for Saccharina japonica Fermentation Hyeon Song Oh and Youn Tae Chi* School of Biological Sciences and Technology, Chonnam National University G033 Effect of Before and After Fermented Dendropanax morbifera Extract on HEK293 and PANC-1 Cells Ra Min Seo and Youn Tae Chi* School of Biological Sciences and Technology, Chonnam National University G034 Comparing the Ingredients and Antioxidant Effects of Dendropanax morbifera Fermentation Using Microorganism Ho Min Song, Sang Hoon Na, and Youn Tae Chi* School of Biological Sciences and Technology, Chonnam National University 194

196 Poster G035 Isolation and Identification of Ground Microorganism to Find New Beneficial Microbial Agents Jin Sung Kim and Youn Tae Chi* School of Biological Sciences and Technology, Chonnam National University G036 Screening and Application of Xylanase Producing Sphigobacterium sp. A10 Strain Sang Hoon Na and Youn Tae Chi* School of Biological Sciences and Technology, Chonnam National University G037 Dyeing Properties and Antibacterial Activity of Natural Pigment from Marine Bacteria Ga Eun Lee and Jin Sook Park* Department of Biological Science and Biotechnology, Hannam University G038 The Physicochemical Stabilities of Pigment Extracts from Erythrobacter sp. PPB2-8 Ga Eun Lee and Jin Sook Park* Department of Biological Science and Biotechnology, Hannam University G039 Development of Home-made Gibson Assembly Enzymes for Do It Yourself Biologists Jaewon Kim, Saeyoung Lee, Hongjae Park, Kyoungwoo Jang, and In-Geol Choi* Department of Biotechnology, Korea Univeristy G042 Proper Regulation of CBP7, a Calcium Binding Protein, is Required for Development in Dictyostelium Dong-Yeop Shin and Taeck J. Jeon* Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University G043 Adaptive Engineering of a Hyperthermophilic Archaeon on CO and Discovering the Underlying Mechanism by Multi-omics Analysis Seong Hyuk Lee 1,2, Min-Sik Kim 3, Jae-Hak Lee 1, Tae Wan Kim 1,2, Seung Seob Bae 1, Sung-Mok Lee 1, Hae Chang Jung 1,2, Tae-Jun Yang 1, Ae Ran Choi 1, Yong-Jun Cho 4, Jung-Hyun Lee 1,2, Kae Kyoung Kwon 1,2, Hyun Sook Lee 1,2, and Sung Gyun Kang 1,2 * 1 Korea Institute of Ocean Science and Technology, 2 Department of Marine Biotechnology, Korea University of Science and Technology, 3 Korea Institute of Energy Research, 4 Chunlab, Inc. G044 Adaptive Evolution of a Hyperthermophilic Archaeon Thermococcus onnurineus NA1 during Growth on Formate Hae-Chang Jung 1,2, Seong Hyuk Lee 1,2, Jung-Hyun Lee 1,2, Hyun Sook Lee 1,2, and Sung Gyun Kang 1,2 * 1 Korea Institute of Ocean Science and Technology 2 Department of Marine Biotechnology, Korea University of Science and Technology G045 DReS: A Direct Cloning Technique Using Bacteriophage Recombination Systems Kyoungwoo Jang, Linh Thuy Do, Hongjae Park, and In-Geol Choi* Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University G040 RapB Controls Cell Adhesion and Migration in Dictyostelium Byeonggyu Park and Taeck J. Jeon* Department of Life Science & BK21 Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University G041 Cytokinesis Defects of rapgap9 null Cells are Rescued by Expressing Truncated GAP-Domain Proteins ARa Lee and Taeck J. Jeon* Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University G046 A Bacterial Cell-based Biosensor Using Bacterial-two Hybrid System for the Detection of Endocrine Disrupting Chemicals Su Hyun Ryu, Yoon Mo Yang, Yeh Eun Lee, and Jin Won Lee* Department of Life Science and Research Institute for Natural Sciences, Hanyang University G047 Engineering Substrate Selectivity by Active-site Residues in Ferulic Acid Decarboxylase of Enterobacter sp. Px 6-4 by Site-saturation Mutagenesis Sunil Ghatge, Youri Yang, and Hor-Gil Hur* School of Environmental Science and Engineering, Gwangju Institute of Science and Technology 195

197 2016 International Meeting of the Microbiological Society of Korea H001 Antiviral Activity of Bisisomahanin from the Glycosmis stenocarpa Jang Hoon Kim 1, Ju Yeon Yoon 1, Seung Kook Choi 1, Sun Jung Kwon 1, In Sook Cho 1, Young Ho Kim 2, and Gug Seoun Choi 1 * 1 Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, RDA, 2 College of Pharmacy, Chungnam National University H002 Marine Fungal Resource Bank Jae Young Park, Myung Soo Park, Ji Eun Eom, and Young Woon Lim* Seoul National University H003 Pan-genomic Analysis of Lactobacillus salivarius Strains as Anti-pathogenic Swine Probiotics Jun-Yeong Lee 1, Geon Goo Han 1, Gwi-Deuk Jin 2, Sang Mok Lee 1, Yun-Jaie Choi 1, and Eun Bae Kim 2,3 * 1 Department of Agricultural Biotechnology, Seoul National University, 2 Department of Animal Life Science, College of Animal Life Sciences, Kangwon National University, 3 Division of Applied Animal Science, College of Animal Life Sciences, Kangwon National University H004 Development of Detection Method for Foodborne Pathogens at Low Density on Fresh Produce Sujin Yun, Jin-Woo Park, Jae-Gee Ryu, and Sanghyun Han* Microbial Safety Team, Department of Agri-Food Safety, National Institute of Agricultural Sciences H005 Relationship between the Microbiota in Different Sections of the Gastrointestinal Tract, and the Body Weight of Broiler Geon Goo Han 1, Jinyoung Lee 2, Jun-Yeong Lee 1, Gwideuk Jin 3, Jongbin Park 4, Changsu Kong 2, Eun Bae Kim 3, and Yun-Jaie Choi 1 * 1 Department of Agricultural Biotechnology, Seoul National University, 2 Department of Animal Science and Technology, Konkuk University, 3 Department of Animal Life Science, Kangwon National University, 4 Department of Animal Life System, Kangwon National University H006 Prediction and Purification of Sesquiterpene Synthase from Wood Rot Fungus Su-Yeon Lee, Jieun An, and MyungKil Kim* National Institute of Forest Science H007 Genetic Analyses of Sinonovacula constricta from Southern Coast of Korea Based on DNA Sequences of Mitochondrial Cytochrome Oxidase I Gene Mi Sun Kim, Ji Hee Lee, Joo Won Kang, Dae In Kim, and Chi Nam Seong* Department of Biology, College of Life Science and Natural Resources, Sunchon National University H008 Evaluation of Bacterial Community Composition of Commercial Animal Probiotics from Korea Using Brcoded Pyrosequencing Baolei Jia, Hyo Jung Lee, and Che Ok Jeon* Department of Life Science, Chung-Ang University H009 A Phylogenic, Functional and Metabolic Analysis of Bacillus velezensis Using Pan Genome Byung Hee Chun and Che Ok Jeon* Department of Life Science, Chung-Ang University H010 Pan-genome Analysis of Leuconostoc mesenterides Reveals Its Metabolic Capabilities and Diversities during Fermentation Hye Hee Jeon and Che Ok Jeon* Department of Life Science, Chung-Ang University H011 Antimicrobial Activity of Essential Oil of Eucalyptus globulus against Fish Pathogenic Bacteria Joon-Woo Park, Mitchell Wendt, Sabrina Hossain, Sudu Hakuruge Madusha Pramud Wimalasena, and Gang-Joon Heo* College of Veterinary Medicine, Chungbuk National University H012 Morphological Changes of MG-63 Cells by Fucoidan Treatment Hyeseon Kim and Taeck J. Jeon* Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University H013 Effectiveness of Periodic Treatment of Quercetin against Viral Hemorrhagic Septicemia Virus Se-Young Cho 1, Yeong O Kim 2, Se Hyun Cho 2, Jong-Soon Choi 3, Joseph Kwon 3, and Duwoon Kim 1,2 * 1 Foodborne Virus Research Center, Chonnam National University 2 Department of Food Science and Technology, Chonnam National University 3 Korea Basic Science Institute 196

198 Poster H014 A Functional Annotation System Based on Functionally Equivalent Protein/Domain Search Algorithm Dong Su Yu National Institute of Ecology H015 Anti-Norovirus Activity from Natural Plant Extract Using a Norovirus Surrogate System Joo Bong Choi 1, Diana Soils Sanchez 1, Hee Jung Lee 2, In Sun Joo 2, Jeong Su Lee 2, and Sung-Joon Lee 1 * 1 Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University 2 Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation H016 The Role of Sir2 in Oxidative Stress Response Changes Depending on the camp-pka Signaling Yeong Hyeock Kim, Woo Sun Song, Woo Kyu Kang, and Jeong Yoon Kim* Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University H017 Immunogenicity and Efficacy of VLP Forming Baculoviral Vaccine against Influenza pdmh1n1 in BALB/c Mice Yong-Dae Gwon, Sehyun Kim, Yoonki Heo, Hansam Cho, Yeondong Cho, Ki Hoon Park, Yuyeon Jang, Jong Kwang Yoon, Hee-Jung Lee, and Young Bong Kim* Department of Bio-industrial Technologies, Konkuk University H018 Enhanced Immune Response for Foot-and-Mouth Disease Virus Vaccine with Granulocyte Macrophage Colony Stimulating Factor-flagellin Adjuvant Yu Yeon Jang 1, Hansam Cho 1, Yong-Dae Gwon 1, Hanul Choi 1, Jaehyuck Heo 1, Gwonsung Joo 1, Joong-Bok Lee 2, Jiwon Choi 1, and Young Bong Kim 1 * 1 Department of Bio-industrial Technologies, Konkuk University, 2 Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University H019 Chronic Repression of mtor Complex 2 Alters the Composition of the Gut Microbiota in Diet-induced Obese Mice Mi-Ja Jung, Jina Lee, Min-Soo Kim, Dong-Wook Hyun, Na-Ri Shin, Ji-Hyun Yun, Pil Soo Kim, Tae Woong Whon, and Jin-Woo Bae* Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University H020 Effects of Freeze-drying Feces on 16S rrna Based Microbial Community Analysis Jungman Kim, Nakwon Hwang, Mincheal Kim, Son G. Nguyen, and Tatsuya Unno* Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University H021 The Roles of Histone H3-K4 Methylation in Morphogenesis of Candida albicans Jueun Kim and Jung-Shin Lee* Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University H022 Histone Residues Play Important Roles for HM Silencing Maintenance in Saccharomyces cerevisiae Soojin Yeom and Jung-Shin Lee* Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University H023 PAF1 Complex Directly Regulates H3K4 Methylation in Saccharomyces cerevisiae Jun-Soo Oh and Jung-Shin Lee* Molecualr Biochemistry Lab, Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University H024 Korea National Microorganisms Research Resource Center Se Joung Yeom and Sang Seob Lee* Kyonggi University H025 Center for Fungal Genetic Resources (CFGR): Housing Plant Pathogenic Fungi for Educational and Research Purposes Yeo Kyoung Yoon and Yong-Hwan Lee* Center for Fungal Genetic Resources, Seoul National University H026 Korean Metagenome Bank for Exploiting Microbial Diversity Jung-Hoon Yoon Department of Food Science and Biotechnology, Sungkyunkwan University 197

199 2016 International Meeting of the Microbiological Society of Korea H027 Microbial Carbohydrate Resource Bank (MCRB) Seunho Jung Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank(MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University H028 Bacteriophagebank KyoungEun Cha and Heejoon Myung* Dept. of Bioscience and Biotechnology, Hankuk University of Foreign Studies H029 Korea Mushroom Resource Bank Jae Young Park, Nam Kyu Kim, Hey Young Choi, Mi Jin So, and Young Woon Lim* Seoul National University H030 Korea Bank for Pathogenic Viruses Ki-Joon Song Korea Bank for Pathogenic Viruses H032 Lichen as a Novel Bioresources in Korea Young Jin Koh and Jae-Seoun Hur* Korean Lichen Research Institute, Sunchon National University H033 Korean Collection for Oral Microbiology Soon-Nang Park, Yun Kong Lim, Eojin Jo, and Joong-Ki Kook* Department of Oral Biochemisty, School of Dentistry, Chosun University H034 Culture Collection of Antimicrobial Resistant Microbes Hyunjin Hong, Hakmi Lee, Minyoung Lee, Yeonhee Lee, and Eunju Shin* Culture Collection of Antimicrobial Resistant Microbes, Department of Biology, Seoul Women s University H035 Korea Environmental Microorganisms Bank Yong Jin Kim and Sang Seob Lee* Research Center Kyonggi University H031 Plant Virus GenBank Ki Hyun Ryu Dept. of Horticulture, Biotechnology and Landscape Architecture 198

200 The 5 th Microbiology Research Festival for High School Students

201 2016 International Meeting of the Microbiological Society of Korea HS-01 Sporosarcina pasteurii 유전자도입형질전환토양세균을이용한토양내수분보유능력개선및식물생장촉진효과 박상윤, Deerfield Academy 팀명 : Big Green / 지도교사 : Ivory Hills S. pasteurii는생물의광물화 (bio-mineralization) 를할수있는포자형성성비병원성세균이다. 이세균의이와같은독특한능력이최근에서야발견됨으로써이러한특성을적용할수있는분야는여전히미개척상태이다. 생물학적건축자재로인공시멘트보강제로서 S. pasteurii를상업적으로적용하는예는있지만, 토양의보수력을개선하는능력에대한증명은아직이루어지지않았다. 따라서본연구는 S. pasteurii이토양결속력을높인다는사실에착안하여건조기토양내보수력을개선시켜물부족은완화시킬수있는지에대해연구하였다. 또한이와유사한특성을가진다른토양세균도있는지선발하였다. 먼저, 토양세균중우레아를포함한 TSA 배지에서생존하는것들만선발한후 S. pasteurii와선발된다른토양세균은우레아를포함한 TSB 배지에서액상배양되었으며, 동일한농도로조절하여양배추씨앗이심겨져있는토양에접종하였다. 그토양에서자란양배추싹의길이와엽록소 a, b를측정하였으며, 토양내보수력은 1 ml씩물을토양에추가하면서새어나오는양을측정하는방법으로진행되었다. 잔디의생존력을측정하기위하여같은농도의세균용액을잔디에더하였고건조상태에서 9일간관찰하였다. 실험결과, S. pasteurii는 TSA, TSB 등우레아를함유한다양한종류의배지에서배양이가능하였으며토양내수분보유능력을가장증가시켰다. 하지만엽록소 a와 b 생산량은낮아식물생장에는도움이되지않는다는것을확인하였다. 따라서수분보유능력이비교적양호하고식물생장에도움을주는토양세균 d를선발하였으며, 16S rrna 방법으로 DNA의염기서열을분석하고 BLASN 테스트를한결과선발된세균이 Bacillus pumilus라는것을확인하였다. S. pasteurii의 plasmid DNA를 heat shock transformation 방법을이용하여삽입하였다. 이렇게형질전환된새로운세균 d-s. pasteurii를토양에넣고건조환경에서양배추씨앗을발아시키고싹을길러본결과엽록소가 B. pumilus를넣고기른양배추보다많았으며토양보수력도상당히증가시켰다. 결론적으로, 본연구를통해 S. pasteurii가생산하는탄산칼슘이토양을결집시켜보수력을증가시킨다는것을증명하였으며식물생장에도움을주는기존의토양세균에이들유전자를도입하여형질전환세균을만들었을때두세균의장점을모두가질수있다는것을확인하였다

202 The 5 th Microbiology Research Festival for High School Students HS-02 청색광에서의 IAA(Indol 3-Acetate Acid) 분해및활성산소소거능에의한 땀세균억제효과 이승은, 서울국제고등학교 팀명 : Blue I / 지도교사 : Chirs Koester 땀이발생하면세균에의해지방산과암모니아가생성되고이로인해악취가운동복, 장비에서나게되며불쾌감과세균번식이일어나게된다. 이를청색광이옥신을분해시킬때발생하는활성산소를이용해땀발생세균을억제시켜악취를제거할수있는지확인하였다. 먼저청색광에의해옥신이분해되고활성산소가생성되는지확인한결과옥신의색이옅어졌으며, 생성된활성산소에의해활성산소소거능이 10% 감소하였다. 이결과를이용해땀세균에처리할경우자외선을조사할때보다 0.027A 만큼의세균이덜발생하였고대장균과살모넬라와같은병원균에대해서는살모넬라의억제효과는거의없었지만대장균은효과적으로억제하였다. 또한옥신이청색광에변색되지않는농도인 100ppm 을하키선수장갑에분무할경우처리하지않았을때보다 3배정도의효과를보이는것으로나타났다. HS-03 식물공장의세균과곰팡이에대한오염을해결하기위한방안에대하여 천승재, Oakridge Secondary School 팀명 : FRESH / 지도교사 : Ms. McCready 식물공장에서사용하는영양배지인 MS (Murashige-Skoog 배지 ) 배지는식물의생장을촉진하지만동시에해로운세균과식물의생장을억제하는곰팡이의증식을가져온다. 이를해결하기위해건강한토양에서분리배양한토양박테리아를 MS (Murashige-Skoog 배지 ) 배지에투여하고대장균으로배지를오염시킨결과대장균으로오염된배지에서생장한식물은보통잎과줄기가대장균으로오염되는데, 토양박테리아가투여된경우에는이러한오염이현저하게감소하는것을확인하였다. 곰팡이의경우항산화제인프로폴리스를사용하여 MS (Murashige-Skoog 배지 ) 에곰팡이포자를접종한결과곰팡이의생장이크게억제되어식물이정상적으로생장할수있었다. 이두가지실험결과를접목하여토양박테리아와프로폴리스로처리한 MS(Murashige-Skoog 배지 ) 배지는곰팡이의증식을억제하고동시에대장균에의한식물의오염을막을수있다는실험결과를얻을수있었다

203 2016 International Meeting of the Microbiological Society of Korea HS-04 옻나무 (Rhus trichocarpa) 와자작나무 (Betula platyphylla var. japonica) 추출물의항균활성및천연보존료개발가능성탐구 이수민, 김윤정, 이사벨고등학교 팀명 : No- 아질산염 / 지도교사 : 김유강 가공육의보존료로사용되는아질산염은체내에서아민과결합하여니트로사민이라는발암물질을만든다. 따라서아질산염을대체할수있는천연항균제의개발을위해옻나무와자작나무추출물의항균활성을알아보았다. 그결과옻나무와자작나무추출물은 Salmonella typhimurium에는항균활성이없었으나 E. coli와 S. aureus에는항균활성을보였다. E. coli와 S. aureus에대한옻나무추출물의최소저해농도는 0.25 mg/ml 와 0.5 mg/ml였고, 자작나무추출물의최소저해농도는 0.5 mg/ml 와 1.0 mg/ml였다. 또한, 옻나무와자작나무추출물은 ph에도안정적이었으며, 열처리에서도항균활성이나타났다. 옻나무와자작나무추출물을실제돼지고기에넣어균의증식억제효과가있는지알아본결과, 균수의증식이 CFU/ml로억제되었다. 이를통해옻나무와자작나무추출물은아질산염을대체할가능성을보였다. HS-05 로돕신발현으로인공적인광영양성을획득한대장균의실험실내적응진화 김현, 하나고등학교 팀명 : Photo Evolution / 지도교사 : 최호진 인공적인광영양성특성을획득한절대화학영양성대장균 (Escherichia coli) 이어떤진화적변화와대사적변화가일어날수있는지를거시적으로알기위해, 광영양성시아노박테리아인 Gloeobacter 유래의로돕신 (GR) 유전자를발현하는부모세대대장균 (PT) 을 1-W LED 전구로상시빛이조사되는배양기에서적응진화를위한연속배양을수행하였다 (1 g/l 포도당을포함하는최소배지, 37 C, 생장속도 μ=0.1 h -1 ). 304세대이후의후손세포들은부모세대의전형적인 rod 모양 (1~2 μm) 과구별되는특징적으로길어진형태로전자현미경사진으로관찰되었고, 후손세포집단의 49.4% 는 5 μm 이상의길이를갖는개체들이었다. 후손세포집단중 8 μm-필터용지에걸러진세포들은 1 g/l 포도당을포함하는최소배지와빛조사조건에서배양하면포도당소비량당생체량수율이 0.60 ± 0.02 g- dried cell weight/g- consumed glucose 으로, 부모세대대장균 (PT) 이보인세포수율 (0.55 ± 0.02 g/g) 보다 9% 증가했다. 수율변화의이유로탄소원에대한조절이후손세대에서더효율적으로변화했을것으로가정하고그실마리찾기위해, 후손세대집단에서분리한 2종의단일콜로니유래의후손세포가젖당배지에서의생장중에 PEP를첨가하여유당가수분해효소의유전자 (β-galactosidase, lacz) 전사변화를추적하여, 부모세대대장균의조절양식과는유의성있는차이를보임을확인하였다. 수율변화의다른이유는후손세대집단에서분리한 2종의단일세포에서나타난유전체의변화에대하여토론한다

204 The 5 th Microbiology Research Festival for High School Students HS-06 애벌레를이용한플라스틱분해세균연구 김현아, 김하연, 하나고등학교 팀명 : 라바 (larva) / 지도교사 : 최호진 버펄로웜, 밀웜, 슈퍼밀웜, 굼벵이, 장수풍뎅이애벌레, 사슴벌레애벌레등여러애벌레를이용해여러폴리머의분해가능성을알아보았다. 밀웜류의스티로폼분해를확인할수있었다. 이들의장내미생물을분리해린코신, 반 코진, 사이톱신, 테라싸이클린, 셉트린, 겐타마이신등 6 가지항생제로항균효과를확인했다. 그결과사이톱신에 의한항균효과및항생제에의한분해속도변화가가장크게나타났다. 기존겐타마이신에의해높은항균효과를보였던 Yu Yang (2015) 의결과와달리퀴놀론계항생제인사이톱신에의한스티로폼분해저해효과가더크게 나타난것으로보아다른스티로폼분해세균의존재가능성을확인할수있었다. Exiguobacterium sp. strain YT2 외에또다른스티로폼분해세균의존재한다면밀웜의외부에서스티로폼분해세균으로지목된 Exiguobacterium sp. 에의한분해가적게일어난이유를설명할수있다고생각된다. HS-07 밭수확량증대를위한미생물생장에최적화된석회비료개발 김다솔, 김지윤, 류혜지, 임재웅, 경산과학고등학교 팀명 : 말달리자 / 지도교사 : 이향선 대부분의농경지는연작과화학비료의사용으로인해산성화되고있는추세이다. 농부는이러한토질개선을위해석회비료를사용한다. 석회비료는토양을개선해주어밭수확량을증대시키는중요한역할을한다. 그러나석회 비료가토양미생물의생장에미치는영향에관한연구는미흡한실정이다. 따라서본연구에서는주로사용되는 3 종류의석회비료가토양미생물생장에미치는영향과식물생장에미치는영향에대해연구하였다. 3 종류의석회가대표적인 5 종의토양미생물생장에미치는영향을알아보고자 UV/vis spectrophotometer 를이 용하여흡광도를측정한후생장률을비교하였다. 그결과생석회 > 고토석회 > 황산석회순으로생장률이높게 측정되었다. 고체배지에서배양한결과또한생석회가가장많이생장하였고황산석회는거의생장하지않았다. 위실험을바탕으로산성화시킨토양에석회를직접뿌려석회가식물생장에미치는영향을알아보았다. 그결과 대부분의식물군에서 CaO ( 생석회 ) 나혹은 CaO ( 생석회 ) 와다른석회를혼합한토양에서높은발아율을보였다. 특히, CaO ( 생석회 ) 100% 에서는모든식물에서공통적으로가장높은발아율을나타냈다. 우리는본연구를통하여석회가토양미생물과식물의생장에미치는영향을알아보았다. 전체적으로생석회가 미생물생장에유리한환경을제공하였고, 이는식물생장에긍정적인영향을미쳤다. 토양산성화를해결하면서미생물생장, 더나아가식물생장에가장유익한석회비료를개발하는것이연구의목적이다. 이로인해작게는한밭토양에서식물의생장을도울수있을것이고, 넓게본다면황폐해진토양에 이비료를뿌림으로써토양을더비옥하게만들고, 후에식물의천이에도영향을미쳐현재인류가겪고있는 사막화문제, 인구개체수증가에의한식량부족문제등을해결할수있을것으로보인다

205 2016 International Meeting of the Microbiological Society of Korea HS-08 토양미생물분석을통한아미그달린분해능탐구 박재희, 윤도현, 김민석, 박시현, 광주과학고등학교 팀명 : 복숭아 / 지도교사 : 김영준 복숭아씨앗내에포함된아미그달린은시안배당체의일종으로아미그달린이가수분해되어생성되는시안화수소산은사람을치사에이르게한다. 본연구는이러한맹독성물질이미생물에의한분해가가능할것이라고예상하여, 복숭아가자생하는토양에서식하는균을이용하여아미그달린분해능을알아보고자하였다. 토양시료에서아미그달린에내성이있는균주 14종을동정및순수분리하였다. 순수분리된균주를아미그달린이포함된전세포반응용액에서반응시킨후 HPLC로아미그달린분해능을정량적으로확인하였다. HPLC 측정결과 7번미생물에서유의적인값을얻을수있었으며이를통해복숭아자생토양에서자라는미생물이아미그달린분해에사용될수있음을확인하였다. 더나아가용해도가매우낮으며종자주변에국소적으로존재하는아미그달린의특성을고려해종자표면을분해할수있는곰팡이를대상으로실험을진행한다면보다본실험의목적에도달할수있을것으로판단된다. HS-09 토양의종류에따른토양세균의탈염기능분석과이를적용한음식물 쓰레기친환경퇴비개발에관한연구 정진운, 한성과학고 팀명 : 음쓰 / 지도교사 : 정진운 음식물쓰레기를퇴비로재활용하기위해서는염분량을줄여야한다. 이에탈염효과가뛰어난토양미생물을활용하여친환경적인음식물쓰레기퇴비제작법을개발하고자하였다. 상토, 갯벌토양으로부터토양세균을채집하고배양하여염도변화를측정한결과음식물쓰레기퇴비화에는염도를줄일수있는상토세균이더적합하다고판단하였다. 상토세균들을액체배지상태로만든후염도변화를측정하고, 탈염기능이뛰어난세균 P. putida와 B. cereus를동정하여인체에무해함을밝혔다. 이후동정한세균을음식물쓰레기에넣고발효하여퇴비를제작하고, 상추를재배할때퇴비로사용하였다. 상추의발아수, 발아길이, 엽록소양, 토양온도등을확인하여제작한음식물쓰레기퇴비의실용성을검증하였고, 질석에상토세균자체를섞어발효시키는방법이가장미생물의양을잘보존하며식물의생장에적합하다는결론을내렸다. 아울러퇴비에서발생하는침출수의오염도를측정한결과일반음식물쓰레기침출수가야기하는환경오염문제를일부감소시킨다는것을확인하였다. 본연구를통해음식물쓰레기의재사용률을높여환경문제를해결하고, 화학비료를대체함으로써토양산성화문제를막을수있을것으로기대한다

206 The 5 th Microbiology Research Festival for High School Students HS-10 자생지별로분리된춘란의난근균근 (Orchid Mycorrihizal Fungi) 이 병원균과자생지에서식하는토양미생물과춘난뿌리에주는영향 남윤성, 홍천고등학교 팀명 : 홍천미생물탐구팀 / 지도교사 : 신은주 난은주변환경에민감해푸사리움과같은곰팡이가뿌리를감염시켜뿌리가마른상태로부패하는근부병, 뿌리가붉은갈색으로변하는뿌리썩음병등에걸린다. 때문에영양제와농약을처리하지만양조절이어렵다. 따라서난뿌리와공생관계에있는난균을이용해곰팡이억제및뿌리의생장을증가시킬수있는지확인해보았다. 그결과군집이흰색이며, 솜털과목화솜형태로배양된난균은작물곰팡이자체를억제시키는것보다는먼저우위를점하고있어다른곰팡이의접근을막아줄수있었다. 또한자생지 5곳에서배양된난균은처음배양한난균과비슷하였고, 자생지의토양미생물과는서로생장에큰영향을주지않았다. 마지막으로난균을난뿌리주변에처리할경우뿌리의 starch와 cellulose 함량을증가시켜뿌리의세포벽을강하게해주었으며, 그대신뿌리에서 sucrose가분해되어생성된 glucose와 fructose를난균이흡수하는것으로나타났다. HS-11 온도변화에따른향신료의 Staphylococcus aureus, Bacillus cereus, Escherichia coli 에대한항균효과 나지윤, 청심국제고등학교 팀명 : Arabia Eudamon / 지도교사 : 김정석 향신료의항균효능은많은연구들을통해서밝혀져왔지만, 온도변화에따라서향신료들의유효성분의항균효능이어떻게변화할지의문이생겨연구하게되었다. 향신료에함유된유효성분은물을용매로사용한속슬렛추출방법을통해추출하였고, 농축및동결건조후물에녹여사용하였다. 각시료는수조에서 25 C, 50 C, 75 C, 100 C로 15분간열처리후, 식히고각각 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml, 2 mg/ml, 4 mg/ml 농도로희석하여사용하였다. Inhibition clear zone 의지름을측정한항균실험결과, 그람음성균주인대장균에는항균효과가나타나지않았으며, 그람양성균주인황색포도상구균과세레우스균에는 4 mg/ml의농도에서만항균효과를보였다. 열처리하지않은고추추출물, 100 C 열처리한마늘추출물, 75 C 열처리한생강추출물과후추추출물의항균력이높게나타났다. 위실험결과를바탕으로열을가해야하는탕류음식을요리할때에는음식이식어감에따라서마늘, 생강, 후추, 고추순으로첨가한다면향신료들의항균기능적인면에서는더효과적일것이라고제안한다

207 2016 International Meeting of the Microbiological Society of Korea HS-12 아무르불가사리 (Asterias amurensis) 추출물의항생및항균효과에대한연구 홍승희, 노예림, 최혜민, 인천진산과학고등학교 팀명 : Asterias / 지도교사 : 김우태 본연구는경기도화성시우정읍국화리에속해있는국화도에서아무르불가사리 10마리를채취하여추출하는것을시작으로하여아무르불가사리의다양한생리활성능력을파악하여불가사리를활용할수있는방안을마련하는것을목적으로진행되었다. 추출물제조이후에는항생, 항균능력이있는지알아보는방법으로 Paper disc method를이용하였으며미생물생장억제능력도있는지알아보는실험을진행하였다. 항균항생활성이있는지알아보기위해사용한미생물을우리주위에서쉽게접할수있는이, 피부, 핸드폰등과같은곳에서미생물을배양하여그람염색과 SDAC 선택배지를통해분류하여사용하였다. 그결과이와빵에서채취한미생물종류에서미약한항균활성이나타나는것을볼수있었고, 미생물생장억제여부는생식소추출물에서 5개균에서생장억제효과를볼수있었다. HS-13 망간산화미생물을이용한새로운미생물전지개발 천성우, 전유진, 천수범, 최민기, 경산과학고등학교 팀명 : Keraunos / 지도교사 : 이향선 망간산화미생물 (MOB) 인 균주를이용하여다니엘전지를이용한전기발생에관한탐구를진행하였다. MOB 를 LB배지에일정량배양을시킨후온도, ph, MOB 주입량에따라서변인통제를하여실험하였다. (-) 전극쪽에는 와 를넣어서전자를주게하고, (+) 전극쪽에는,, 와 를넣어서전자를받게하였고, 가운데에는염다리 ( ) 를넣어서분극현상을없애주었다. 는염기성환경에서주로활동하는것으로알려져있다. 위의 는 반응을이용하여전기를생산하는우리실험에서변인 를통제하기위하여종류별로사용하였다. 본실험에서는 인터페이스의전압센서를전압검출에이용하였으며, 실시간으로측정하였다. 또한, 전압효율계산식을이용하여최대효율을발휘할수있는변인들을찾아서가장큰전압을생산해낼수있는상황을추정하였다. 현재연구되고있는미생물전지는모두간접미생물전지로미생물의활동으로인하여발생하는전자를이용하여전기를생산해내는방식이다. 그러나우리가실험한미생물전지는미생물의활동이직접적으로발전하는데영향을주는직접미생물전지이다. 아직연구되지않은직접미생물전지탐구를통해간접미생물전지를이용하였을때보다높은효율을얻을수있었다. 따라서우리는직접미생물전지의하나의방법을스스로개발하여더좋은성능의전지를만들수있다는가능성을얻었다

208 The 5 th Microbiology Research Festival for High School Students HS-14 생물독소를이용한김치유산균과유제품유산균의업그레이드 - 장까지살아서가라!!!- 조서원, 은광여자고등학교 팀명 : L.A.B / 지도교사 : 임덕린 김치유산균과비교하여낮은 ph 와저온, 다른세균의독소에대한저항력, 그리고과산화수소의생성과과산화수소에대한저항성, 항생제에대한저항성이크게부족한발효유에존재하는유산균을분리배양하여 24 개의유산균주를확보하였고이들을대장균의독소에적응력을가지게하고, 프로바이오틱스기법으로김치유산균의파쇄물이존재하는배양배지에서배양을실시하여김치유산균과비슷한능력을가지는 3 종류의유산균주를분리배양할수있었다. 이들을이용하여김치를제조한경우에도김치의숙성후채취한유산균들이낮은 ph 와저온, 그리고다른세균의독소에대한저항력, 그리고과산화수소의생성과과산화수소에대한저항성, 항생제에대한저항성이증가한유산균임을확인할수있었다. HS-15 자성이 Magnetospirillum magnetotacticum에미치는영향및활용방안탐구 권영광, 이준희, 최지훈, 이승준, 경산과학고등학교팀명 : Magneto / 지도교사 : 이향선 주자성세균은지구자기장에반응하는미생물이다. 이세균은내부에마그네토솜 (Magnetosome) 이라는자철석이있어서자성을띤다. 우리는주자성세균의이러한특징을활용하여자성이 Magnetospirillum magnetotacticum 이라는주자성세균에미치는영향및활용방안에대해여러가지탐구를해보았다. 첫째, Magnetospirillum magnetotacticum 에자기장을걸어주게되면자기력선에의해영향을받아번식속도에영향을받을것이고그결과 Magnetospirillum magnetotacticum 의배열에도영향을받을것이라고예상하여, 자기장세기에따른 Magnetospirillum magnetotacticum 의증식속도변화및배열상태를분석했다. 기대했던것과달리자기장에의한생장속도의변화는거의나타나지않았지만, 생장이아닌철분의포착같은다른기능에영향을주었을가능성을생각해볼수있다. 둘째, 미세물질 ( 철가루 ) 과 Magnetospirillum magnetotacticum 을배양한배지를함께넣으면자기장을띠는미생물에의해미세물질의위치가변할것이라고예상하여, Magnetospirillum magnetotacticum 을이용한미세물질의이동을측정했다. Magnetospirillum magnetotacticum 을배양한배지는그렇지않은배지에서보다철가루가유동적으로이동했으며이를활용하면미세물질을원하는위치로이동시킬수있는장치를만들수있다. 셋째, Magnetospirillum magnetotacticum 에부착된미세철가루와짚신벌레가함께있다면진핵생물이미생물을섭취할때철가루가함께섭취가될것이라고예상하여, Magnetospirillum magnetotacticum 로의한짚신벌레의표지방안에대해탐구했다. 철가루를부착시킨 Magnetospirillum magnetotacticum 과짚신벌레를함께배양한후자기장을걸어주어짚신벌레분리도를관찰한결과기존의생물표지방식을개선할수있다는가능성을얻었다. 우리는주자성세균을이용한다양한실험결과를통해활용방안을제시했다. 추후고자기장실험환경같은여러요인들을고안하여추가실험을실시하고, 실제로활용할수있는구체적인방안을알아보고자한다

209 2016 International Meeting of the Microbiological Society of Korea HS-16 배양환경에따른 Monascus ruber 의색소생산량탐구 이상원, 성지환, 이준석, 이창호, 경산과학고등학교 팀명 : MONA / 지도교사 : 이향선 우리팀은최근인공색소의유해성과발암성이밝혀지면서천연색소에대한관심과기대가높아지고있다는것을알았다. 따라서인공색소를대체할수있고, 의학, 염색등다양한분야에서사용되어지고있는천연색소를보다효율적으로생산할수있는조건을찾기위해서탐구를진행하였다. 인체에안전한색소를생산하는것으로알려진홍국균의일종인 Monascus ruber를이용하여여러가지환경변인에따른색소의생산량을측정하고가장생산량이많은조건을찾아보았다. 균주를일정량 Lin Medium 배지에접종하여배양시키고 ph, 접종량, 빛, 소리에따른영향을파악하기위해서각각의변인을통제하여실험을진행하였다. 색소의양을측정하는데에는 uv/vis spectrophotometer를이용하였으며 2일에 1번간격으로측정을실시하였다. 초기 PDB 배지에의배양을통해질소와인산의존재가생산량에영향을끼친다는것을알았으며, 시간이지나면서배지의색이붉어지는것을육안으로확인했다. 측정한흡광도값을바탕으로할때 Monascus ruber이약한산성또는염기성에서중성보다높은생성률을보였으며, 접종량을다르게했을때초기에다양한값을보였으나최종적으로는값이비슷해지는경향을보였고 0.2 ml를접종했을때가장생성률이높았다. 빛을비추었을때색에따라생성량이달라지는것을확인하였으며청색에서가장생성률이높았다. 흡광도측정을진행할때초기에일부배지에서 10,000 rpm에서원심분리시켜도가라앉지않는작은입자들이있었는데이것이흡광도의측정시에측정값을높여오차를크게만드는역할을하는것으로보인다. 더높은 rpm 에서더오랜시간원심분리함으로써이문제를해결하면더정확한값을측정할수있을것이다. 또한다른환경변인을통한실험결과와이실험결과를이용해서발효시간이필요한홍국쌀을최적조건에서배양을함으로써홍국쌀을만드는데필요한발효시간을단축시키고효율을증대시켜현재보다더많은양을생산할수있도록할수있을것이다. 또한다른균주에서도최적의생산조건을찾음으로써색소생산량을증대시킴으로서의학용으로사용되어지고있는다양한색소의보급을용이하게하고인공색소를대체할천연색소를더많이생산할수있게만들어줄것이다

210 The 5 th Microbiology Research Festival for High School Students HS-17 쥐장내미생물의개체수변화를통한황토의식용가능성탐구 정현지, 윤소희, 송종민, 동패고등학교 팀명 : OCHER / 지도교사 : 송석환 흙속의효소에대한연구자료에따르면황토한스푼에는약 2억마리의미생물이살아있다고한다. 또한황토는건축자재, 화장품, 전통약품등다양한방면에서유용하게쓰인다. 하지만황토특유의장점에도불구하고황토를식품으로개발한다는논문자료는거의없었고, 황토를투여하였을때, 쥐의장내미생물의개체수변화과정을본연구는없었다. 이를바탕으로, 흰쥐에게황토를급여해장내유익균과유해균의개체수변화를조사하여황토의식용가능성을알아보았다. 또, 황토의열처리유무가쥐장내미생물에끼치는영향을알아보았다. 실험Ⅰ에서황토의멸균여부를달리하여쥐변원액을도말한배지에 disc 확산법을적용한결과멸균하지않은황토의미생물이 LB 배지의균을우점하고 MRS 배지의유산균생장을덜저해시키는것으로확인되었다. 실험Ⅱ 에서황토물의농도를달리하여실험 Ⅰ과마찬가지로실험한결과 % 의농도가가장유산균의생장을덜저해시키는것으로밝혀졌다. 실험Ⅲ 에서는흰쥐에게황토물을직접음수투여해변을희석도말한결과대장균의수는증가하였으나전체적인유해장내미생물의수는감소하였고, 유산균두종의수가증가한것으로보아, 황토는장건강에도움을주고식용가능성이어느정도있다고판단하였다. HS-18 스테비아발효액을활용한볏짚사일리지첨가제개발 윤이성, 세종과학예술영재학교 팀명 : SEMA (Stevia EM Appliers) / 지도교사 : 권영식 예전에는벼농사후남는볏짚을불에태우거나다시논에썰어넣는등거름용으로주로활용해왔지만최근 10년간볏짚은소먹이용으로생산되기시작하였다. 수입조사료에비하여가격이월등히저렴하기때문에많은축산농가들이소먹이용으로활용하고있다. 그러나최근농약이묻은볏짚을먹고 49마리의소가집단폐사하는안타까운사연이보도된바있다. 한중 FTA 체결등가뜩이나어려운농촌환경에서축산농가및볏짚을생산하는업체모두견디기힘든소식이아닐수없을것으로생각이든다. 또한소고기를즐겨먹는식생활변화로인하여언제어디서든이러한농약들을간접적으로섭취하게된다고생각하니안전성에대한의문이들었다. 이를계기로볏짚생산시잔류농약을효과적으로제거하기위한방법에대해서인터넷검색을진행해본결과스테비아의발효액이잔류농약제거에탁월할것으로생각이들어서이를직접실험을하게되었다. 놀랍게도스테비아발효액을반응시킬경우기존의상용발효액에비해살충제의주성분인 Fenobucarb의함량이전혀검출되지않았음을확인할수있었다. 이는스테비아발효액성분이잔류농약성분을제거하였기때문으로풀이된다. 이결과는스테비아의잔류농약제거효능에대한입증뿐만아니라앞으로볏짚을생산할경우스테비아발효액을활용한다면잔류농약에대한걱정없이안전한소먹이가탄생될것으로기대된다. 또한이를통해국내한우섭취에대하여믿고안전하게이용할수있는계기가될것으로기대된다

211 2016 International Meeting of the Microbiological Society of Korea HS-19 폐식용유에서분리한지방분해효소생산균이에어퍼프의오염도에미치는영향 이지수, 이정아, 초당고등학교 팀명 : TOB / 지도교사 : 류진아 본연구에서는폐식용유에서지방분해효소생산균을추출하고, 지방분해효소활성측정을통해지방분해효소생산균의효율성을입증하였다. 배양시간, 배양액의 PH, 배양액에첨가된지방의종류별로변인통제를설정해지방분해효소생산균의배양최적조건을확인하였다. 또한, 피부미용및환경에악영향을미친다고알려진에어퍼프의미생물오염도를측정하는실험을통해현재위생상태를파악하고, 이를지방분해효소생산균이포함된일반세정제로에어퍼프를세척하여지방을분해정도를파악하며일반세정제보다지방분해효소생산균이포함된세정제가에어퍼프에포함된얼굴의피지성분을분해하는데효과적이라는결과를입증하였다. HS-20 유산균과생약 ( 식물추출물 ) 의항균력을이용한기능성저염간장연구 이용준, 민족사관고등학교 팀명 : Two Dragons / 지도교사 : 나종욱 간장은한식요리에거의대부분첨가되는우리나라의대표양념중의하나이다. 염도를조절하고특유의향과맛으로음식의맛을감칠나게해주는것으로많이사용되지만, 국물요리와찌개등이많은우리나라의식단에다량첨가되다보니염도가높다는단점이있다. 이에건강에영향을크게미치지않으며영양이나맛에도변함이없는저염간장을만드는것을고안하게되었다. 본탐구는이를예방하기위해유산균을첨가하여간장의보존력을높이고자고안을하였다. 다양한유산균과시중의프로바이오틱스, 유제품의유산균을배양한결과, 그중 Lactobacillus acidophillus와프로바이오틱스가가장생존력이좋았다. 염분의농도가낮을수록생존율이좋았으나저염간장의농도인 7% 에서도높은생존율을보였다. 생약을첨가한배지에서식품오염세균과같이배양하였을때 Lactobacilus acidophillus의항균력이좋다는것을알수있었다. 황기추출물에서가장항균결과가좋은것으로봐서황기추출물과 Lactobaciilus acidophillus의혼합물이가장효과가좋은것으로확인되었다. 이를통해저염간장에유산균을첨가하여만든간장은생약에의한약리작용뿐아니라보존력도유지가된다는것을알수있었다

212 The 5 th Microbiology Research Festival for High School Students HS-21 무좀과발냄새원인균에대한녹차찌꺼기의직접적인적용에대한탐구 이현탁, 한종욱, 민족사관고등학교 팀명 : 강고철자 / 지도교사 : 나종욱 This research is to see the optimal condition using green tea leaf (GTL) and green tea bag (GTB) to stop the proliferation of Trichophyton rubrum (TR), Micrococcus luteus (ML), Staphylococcus epidermidis (SE) which are well known as foot odor/ athlete s foot causing microorganisms. After incubation, TR, ML and SE were cultured with GTL and GTB. Brewing time, moisture and applied time of GTL/GTB were control factors. Results indicated that GTL/GTB didn t show significant antimicrobial effect on TR, ML and SE, so we conducted additional experiment using paper disk method, increased quantity and surface area of GTL/GTB. Similar to the previous experiment, results showed no significant antimicrobial effect. These results showed that directly applying GTL/GTB on plates doesn t have antimicrobial activity on TR, ML and SE. We concluded that GTL/GTB it didn t show enough antimicrobial activity because it has low concentration of catechin. The value of this research is that it is the first attempt to apply unprocessed green tea leftovers. Unlike other researches, this study is directly related to our daily lives. Also, this research is the first to cover all TR, ML and SE which are known as foot odor/ athlete s foot causing microorganisms. HS-22 슈도모나스엘로데아 (Pseudomonas elodea) 를이용한식물생장및토양변화에관한연구 조수민, 김성현, 창원과학고등학교 팀명 : 마음의소리 / 지도교사 ; 정원준 본연구는가뭄으로인한토양의황폐화를완화하기위해토양의수분흡착력, 수분보존력을높이는연구를수행하였다. 토양의수분흡착력과수분보존력의강화를위해점성을지닌 gell을형성하는슈도모나스엘로데아 (Pseudomonas elodea) 를처리하였다. 토양의수분흡착력, 토양의건조중량을비교하여토양상태를확인했고, 식물의줄기길이, 식물의흡광도를비교해식물의상태를확인했다. 이를바탕으로슈도모나스엘로데아가토양환경개선에얼마나긍정적인영향을끼쳤는지분석하였다

213 2016 International Meeting of the Microbiological Society of Korea HS-23 빛의파장에따른 melatonin 과장내세균의상관관계탐구 이주예, 김보람, 박연지, 최희승, 유성여자고등학교 팀명 : 모캄모 / 지도교사 : 이호윤 면역력 (immunity) 은우리의건강과직결되는가장중요한부분중하나이다. 면역력은우리몸의다양한요인에의해증가되기도, 감소되기도하는데, 이에대표적으로라토닌 (melatonin) 이라는호르몬과장내세균이있다. 본연구는면역력을연결고리로하여각각다른색의빛의파장에따른멜라토닌수치와장내세균수의상관관계가있을것이라고예상하고, 이에맞게실험을설계하여약 8주가량실험을진행하였다. 먼저, Red, green, Blue, White LED light를설치하고이에맞게각그룹씩마우스를나뉘어주기적으로 LED를쬐었다. 이후, 장내세균수를측정한결과, 약 540nm의파장인녹색파장에서가장많은장내세균수가측정되었다. HS-24 귀부와인의 NO.1 Botrytis cenerea, 음식물쓰레기의세계로진출하다. 최현빈, 이재연, 경산과학고등학교 팀명 : 샤도디켐 / 지도교사 : 이향선 귀부와인은 Botrytis cenerea 의작용으로부패된포도를사용하여만든것으로, 독특한향과달콤한맛으로많은사람들에게사랑을받고있다. 특히와인의생성에서수분을제거해당도를높여주고독특한향을만들어주는 Botrytis cenerea 는잿빛곰팡이의병원균으로, 많은농작물에치명적인피해를입힌다. 우리는이러한 Botrytis cenerea의양면성에대해흥미를느꼈고, Botrytis cenerea를이용하여음식물쓰레기처분에효과적으로사용할수없을까생각하여탐구하게되었다. 이에따라우리는음식물쓰레기의수분을줄일수있는지와수분감량에의해유해균감소효과가있는지의여부, 두가지로나누어실험을진행하였다. 먼저, 수분감소효과를알아보기위해서아무것도넣지않은과일을대조군으로, Botrytis cenerea 를주입한과일을실험군으로두었다. 그리고유해균감소효과는실험군으로 E. coli를주입한과일을사용하였다. 총 A, B, C, D 네가지실험을귤, 고추, 딸기이세작물을대상으로진행한결과, Botrytis cenerea 로인한유해균감소효과에대해서는큰경향성을파악하기어려웠으나, 수분감소면에있어서 Botrytis cenerea의작용을확인할수있었다. 이연구결과를활용하여미생물에의한발효와분해방식의기존음식물쓰레기처리법을개선할수있다. Botrytis cenerea 를이용하여음식물쓰레기의내부수분을감소시키는처리방식은곰팡이의생장기간을보았을때가정보다는대단위음식물처리장에더적합할것이다

214 The 5 th Microbiology Research Festival for High School Students HS-25 식중독을유발하는균에대한식물성기름의항균효과및원인성분에대한탐구 김소윤, 오재석, 청심국제고등학교 팀명 : 올리브오일 / 지도교사 : 구수연 식품의부패와식중독균성장을억제하기위하여생선의표면에올리브유를발라보관하는경우가있다. 본연구는이러한전통적인지혜를토대로하여다섯종류의식물성기름, 이를구성하는성분여섯종류, 그리고식중독원인균세종류를선정하여 agar well diffusion method와 paper disc method를사용하여항균효과를실험하였다. Agar well diffusion method로실험한참기름, 올리브유, 카놀라유, 포도씨유, 해바라기씨유의항균테스트에서는 clear zone이나타나지않았다. Paper disc method로실험한해당기름의구성성분으로밝혀진 linoleic acid, oleanolic acid, oleuropein, lignin, stearic acid, oleic acid의항균테스트에서는 oleanolic acid를제외한성분에서 clear zone이관찰되었다. 그람양성균과음성균으로나누어분석해본결과, 세포벽의두께와무관하게하게항균물질의생장억제효과가있음을도출할수있었다. Oleic acid와 linoleic acid의비율을조절하여시너지테스트를추가시행한결과 oleic acid의비율이높을수록더강한시너지가발생함을관찰하였다. 이를통해 oleic acid의조성비율이가장높은올리브유의항균성을간접적으로증명할수있었다

215 2016 International Meeting of the Microbiological Society of Korea HS-26 경골어류와연골어류의숙성과정에서의미생물차이연구및이를이용한 경골어류숙성방안탐구 우재연, 김동연, 민족사관고등학교 팀명 : 우동 / 지도교사 : 나종욱 삭힌홍어는그취향에따라평가가상이하게갈리지만, 한번그특유의톡쏘는맛에반하고나면쉽게그맛을잊지못한다고한다. 뿐만아니라홍어는영양면에서도탁월한전통식품이다. 이렇듯홍어는많은이들에게사랑받는식품이지만그가격때문에사람들이쉽게접하지못하는것이현실이다. 이러한홍어를보다저렴한가격에먹을수는없을까고민하던중, 채식주의자들이콩, 쌀, 밀등의비슷한식감의다른재료를이용하여식물성고기를만들어섭취하는것을보고다른어류를이용하여삭힌홍어의대용품을만들어보고자실험을진행하게되었다. 특히본실험에서는경골어류인가자미를이용하여대용품을생산하는것에주목했는데, 대체로경골어류가연골어류보다저렴하다는점에착안하여, 저렴한가격으로비슷한식감을가지는홍어대용품을만들고자했다. 연골어류의특성을경골어에최대한반영하기위하여우선연골어류, 그중에서도홍어의삭혀지는과정에주목하였다. 우선홍어시료가삭힌홍어의톡쏘는맛을내는암모니아가홍어를삭히는과정에서어떻게변하는지알아보기위해시간의경과에따른홍어시료의 ph 변화를조사하였다. 이러한과정에서발생하는암모니아가강알칼리성환경을조성해삭힌홍어에세균이살지않는다고알려져있는데, 과연삭힌홍어에서세균이전혀살수없는지확인해본다. 만약이러한예상과는반대로삭힌홍어에도세균이존재한다면, 그세균은어떠한종류의것들인지알아본다. 이후, 위탐구로부터얻은연골어류를삭히는원리를보다값싼경골어류에적용해저렴한대용식품을만들어보기로한다. 대용식품을만들기위해다음두가지방법을생각해보았다. 1 홍어시료와대용식품의재료 ( 가자미 ) 를같은공간에서함께삭혀서홍어시료에존재하는암모니아나발효 세균들이대용식품재료 ( 가자미 ) 를삭힐수있도록하였다. 2 경골어류에 ( 연골어류의삭힘에기여하는핵심성분인 ) 요소를가하여삭힌다. 이때, 1, 2( 이하대용식품후보군 ) 의 ph 를확인함으로써대용식품후보군들이삭힌홍어와같이강알칼리성 을띠는지확인한다. 또한대용식품후보군을각각세균동정하여식용으로안전한지조사한다. 대조군인가자미에 대해서도 ph 탐구, 세균동정을동시에진행한다

216 The 5 th Microbiology Research Festival for High School Students HS-27 Sulfate-reducing Bacterium 과 Photosynthetic Bacterium 을이용한 농작물재배과정중의논산성화방지 이민행, 김영현, 유상훈, 조옥현, 하나고등학교 팀명 : 인연 / 지도교사 : 최호진 본실험팀은미생물을이용한토양산성화문제해결방안을마련해농작물재배과정중의논산성화를방지하고자했다. Sulfate-reducing Bacterium과 Photosynthetic Bacterium이본연구의핵심소재이다. Sulfate-reducing Bacteria가토양속황산이온을분해하고, 이때생성된독성물질 를 Photosynthetic Bacteria가분해하여,, 로바꾸어준다. 이과정이원활하게이루어진다면미생물을이용하여토양산성화를해결할수있는획기적인방식이탄생하는것이다. 하지만실험에사용된 Sulfate-reducing Bacterium과 Photosynthetic Bacterium이혐기성세균이었기때문에배양에연이어실패하였다. 결국연구가지속되지못하고중단되고말았지만, 본연구팀은이연구주제가매우창의적이라고생각한다. 따라서뛰어난연구환경을갖춘뒤재개할가치가충분한연구라고생각한다. HS-28 제주자생식물추출물을이용한무좀치료효과탐구 김민진, 김동건, 김연진, 경산과학고등학교 팀명 : 제주많은무좀약 / 지도교사 : 김민진 무좀은여러균에의해복합적으로일어나는데, 그중주원인이되는균은 Trichophyton Rubrum, 적색백선균이다. 본연구에서는해당균주의생장을억제하는방법을찾는것을주목적으로두었다. 항산화력이강한제주자생식물중예덕나무와비파나무의잎, 밤나무의껍질을선정하였고, 기존치료요법의효과를측정하기위해피톤치드, 식초, 연고를선정하여실험에사용하였다. 식물들의추출물을만들고, 피톤치드와식초, 연고와함께배지에각각 5%, 10%, 20% 농도로첨가하여각성분이첨가된배지에 T.rubrum을배양한결과, 예덕나무잎추출물이 20% 포함된배지와연고가 10%, 20% 배양된배지에서만균이자라지않은것을확인할수있었다. 생장억제효과는두물질모두일정농도이상이되면 Colony 형성이되지않았기에효과는비슷하다고결론내렸다. 또한 T.rubrum 의특징을보다자세히알기위해 SEM을사용하여균사구조를촬영하였다. 특수배지중피톤치드가첨가된배지에서는 Colony가붉은색으로형성되었는데, 균사가형성되지않아특이점을알아보기위해 Colony를 SEM으로촬영하였다. 그결과, 균사구조와 Colony 구조의차이를관찰할수있었다. 종합적으로위결과를통해기존에사용하던치료방법에비해효과가떨어지지않으면서도자극적이지않고무해한치료법을얻을수있을것으로기대된다

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218 Exhibition

219 2016 International Meeting of the Microbiological Society of Korea Exhibition Date: April 20 (Wed)-April 22 (Fri) Place: Kimdaejung Convention Center, Convention Hall Lobby Booth Layout 218

220 Exhibition Booth No. Exhibitors 01,02. 에스피엘 / SPL Life Sciences Co., Ltd. 03. 기산바이오 / Kisan Bio Co. Ltd 04. 바이오니아 / Bioneer Corporation 05. 이바이오젠 / E-biogen 06. 성우제네텍 / Sung Woo Genetech Inc. 07. 벡톤디킨슨코리아 / Becton Dickinson Korea 08,09. 에펜도르프코리아 / Eppendorf Korea Ltd. 10. 에스코코리아마이크로피티이 / Esco Korea Micro Pte 11. 한국애질런트테크놀로지스 / Agilent Technologies Korea Ltd. 12. 서린바이오사이언스 / SeouLin Bioscience Co., Ltd. 13. 엔지노믹스 / Enzynomics, Inc. 14. ( 주 ) 천랩 / ChunLab, Inc. 15. 디엔에이링크 / DNA Link USA, Inc. 16. 마크로젠 / Macrogen Inc. 17. 다인바이오 / DYNEBIO INC. 18. 바이오디 / BioD Co., Ltd. 19. 메가바이오 / MegaBio Co., Ltd. 20. 한국로슈진단 / Roche Diagnostics Korea 21. 신영코퍼레이션 / ShinYoung Corporation 22. 솔젠트 / Solgent co., Ltd 23. 한국벡크만쿨터주식회사 / Beckman Coulter Korea Ltd. 24,25. 주식회사라온테크 / RAONTECH Co., ltd 26. 한국생명공학연구원생물자원센터 / Korean Collection for Type Cultures (KCTC) 27. 제노믹베이스 / GenomicBase Inc. 28. ( 국가연구소재 ) 미생물거점센터 / Korea National Microorganisms Research Resource Center(KNMRRC) 29. ( 재 ) 발효미생물산업진흥원 / Microbial Institute for Fermentation Industry 30. 아이셀 / icell Co. 31. 고마바이오텍 / KOMA BIOTECH 32,33. 우성크라이어텍 / WOOSUNG CRYOTECH CO., LTD. 34,35,38,39. 싸토리우스코리아바이오텍 / Sartorius Korea Biotech Co., Ltd. 36. 엠지메드 / MGMED, Inc. 37. 한일사이메드 / Hanil Sci-Med Co.,Ltd. 40. 케이비티 / Korea Bio-Tech Co., Ltd. B01. 라이프사이언스 / Life Science Publishing Co. B02. 주식회사범문에듀케이션 / PanMun education 219

221 2016 International Meeting of the Microbiological Society of Korea SPL Life Sciences Co., Ltd. Booth No. 01,02 에스피엘 Homepage: CEO: Sang Oh Heo Tel.: Fax: Address 26, Geumgang-ro 2047 beon-gil, Naechon-myeon, Pocheon-si, Gyeonggi-do 11192, Korea Main Technology and Products SPL Life Sciences Co., Ltd. is a leading manufacturer/exporter of scientific plastic lab-ware of Korea. Since established in 1987, we dedicated ourselves towards in manufacturing high quality plastic lab-ware consistent to the finest standards in industries. With a team of enthusiastic professionals continuously engaging in development, quality control of our products fully comply with the stringent ISO This makes our products possible to not only meet, but surpass the international requirements. Kisan Bio Co. Ltd. Booth No. 03 기산바이오 Homepage: CEO: Ji Woon Sun kisan@kisanbio.com Tel.: Fax: Address 2F, Kisan B/D, 86-2, YangJae-Dong, SeoCho-Gu, Seoul 06746, Korea Main Technology and Products Kisan Bio Co., Ltd. is specialized company of Microbial media, ready media, microbial identification and plant tissue culture media. We provide various standard microbial media according to the MFDS, QIA, KP and NIER. Also, for matter of quality and convenience, we provide ready media which can be customized with various options. Bioneer Corporation Booth No. 04 바이오니아 Homepage: CEO: Han-oh Park sales@bioneer.com Tel.: Fax: Address 8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Korea Main Technology and Products Bioneer Corporation is the Korea s leading biotech company that has developed varied Life Science research products and services based on its own technologies. Bioneer is currently providing domestic and overseas researchers with: oligonucleotides, amplification reagents, nucleic acid and protein purification products, gene synthesis and sequencing services, DNA ladders and markers, instruments including conventional and real-time quantitative thermal cycler, fully automated protein synthesis and nucleic acid extraction system, and much more. E-biogen Booth No. 05 이바이오젠 Homepage: CEO: Sungduk Yu service@e-biogen.com Tel.: Fax: Address #305, Ace Hightechcity 2, 25, Seonyu ro 13 gil, Yeongdeungpo gu, Seoul 07282, Korea Main Technology and Products EBIOGENprovidesNextGenerationSequencing(RNA-Seq,DNA-Seq),Microarray,Antibodyarray,qPCRArray,qRT-PCR&ELISAexperimentandDa taanalysisservice. Also Ebiogen provides microbiologists with bacterial related products (Growth-LB broth etc., Preparation-plasmid prep kit & Storage - drying buffer)

222 Exhibition Sung Woo Genetech Inc. Booth No. 06 성우제네텍 Homepage: CEO: Jeong-Wan Hong Tel.: Fax: Address Room. 301, Mega-center, SKn Technopark, 124, Sagimakgol-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do 13207, Korea Main Technology and Products We are a main distributor of Merck Millipore Corp. in South Korea since nineteen ninety nine (1999) and importing/selling many kinds of processing and laboratory devices. Our business fields are Pharmaceutical, Medicine, Biotechnology, Food, Cosmetic industries and many kinds of research institute of national and university. Main product s layout is based on main Merck Millipore items such as water purification systems, industrial pilot scale filtration and purification systems, lab consumable and analysis reagent and kit, and so on. Becton Dickinson Korea Booth No. 07 벡톤디킨슨코리아 Homepage: CEO: Chung Ho Kim KiYong_Lee@bd.com Tel.: Fax: Address 4th Floor, Im sung Bldg. 4 Nonhyeon-ro 64-gil, Gangnam-gu, Seoul 06231, Korea Main Technology and Products DCM(Dehydrated Culture Media) BD offers media with a proven record of performance backed by over 180 years of combined Difco, BBL and Bacto expertise. Excellence starts with our integrated peptone production at the BD Difco Detroit, Michigan manufacturing facility and continues at our new, DCM, Peptone/Hydrolysate milling and blending plant located in Sparks, MD. Here we bring together these ingredients to formulate the wide variety of Difco and BBL products you ve come to trust. PPM(Prepared Plate Media) BD manufactured dehydrated media is used in every prepared media formulation. Each medium is tested against a battery of control organisms, both for growth and, when required, inhibition. BBLTM GasPakTM Products and Accessories BD GasPak EZ Container Systems offer waterless, catalyst-free convenience for use in producing anaerobic, microaerophilic or CO2-enriched environments. Eppendorf Korea Ltd. Booth No. 08,09 에펜도르프코리아 Homepage: CEO: Christian Groeger cs@eppendorf.kr Tel.: Fax: Address Gala Tower 10F, 46 Nonhyeon-ro 85-gil, Gangnam-gu, Seoul 06235, Korea Main Technology and Products Eppendorf product range includes pipettes and automated pipetting systems, dispensers, centrifuges, mixers, spectrometers, and DNA amplification equipment as well as ultra-low temperature freezers, fermentors, bioreactors, CO2 incubators, shakers, and cell manipulation systems. Consumables such as pipette tips, test tubes, microtiter plates, and single-use bioreactor vessels complement the range of highest-quality premium products 221

223 2016 International Meeting of the Microbiological Society of Korea Esco Korea Micro Pte Booth No. 10 에스코코리아마이크로피티이 Homepage: CEO: Chan Won Bae Tel.: Fax: Address 307, 55, Digital-ro 33-gil, Guro-gu, Seoul 08376, Korea Main Technology and Products Esco s Airstream Class II Type A2 Biological Safety Cabinet which is NSF 49 and UL certified is one of the best choices for your laboratory. Now equipped with 70% energy savings DC ECM Motor and Sentinel Gold Microprocessor Controller to display all information on its screen. With its Dynamic Chamber, ISO Class 3 work zone and ISOCIDE powder coat on all its external and interior painted surfaces, you are ensured that you, the operator, as well as the product and the environment are protected from harmful biological agents. Agilent Technologies Korea Ltd. Booth No. 11 한국애질런트테크놀로지스 Homepage: CEO: KIM AUSTIN JUN omics@omics.co.kr Tel.: Fax: Address 98, Hannam-daero, Yongsan-gu, Seoul 04418, Korea Main Technology and Products Agilent is a leader in life sciences, diagnostics and applied chemical markets. The company provides laboratories worldwide with instruments, services, consumables, applications and expertise, enabling customers to gain the insights they seek. Agilent s expertise and trusted collaboration give them the highest confidence in our solutions. SeouLin Bioscience Co., Ltd. Booth No. 12 서린바이오사이언스 Homepage: CEO: Eul Moon Hwang AT@seoulin.co.kr Tel.: , Fax: Address 4F. #A, KOREA BIO PARK, 700 Daewangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13488, Korea Main Technology and Products Leading Total Solutions in Life Science Seoulin Bioscience Sysmex-Partec/Affymetrix (GeneChip microarray, USB, e-bioscience)/bmg/sarstedt/hoefer/lab-bubble/aerte Enzynomics, Inc. Booth No. 13 ( 주 ) 엔지노믹스 Homepage: CEO: Yun il Suh info@enzynomics.com Tel.: Fax: Address HANSIN S-MECA 214~216, 65, Techno 3-ro, Yuseong-gu, Daejeon, Korea Main Technology: Recombinant Protein, Protein Purification, Cloning & Mutagenesis, Customizing/Optimizing Diagnostic Reagents. Products: More than 250 kinds of enzymes with highest purity, i.e. Restriction Enzymes, Polymerases, Reverse Transcriptases, qpcr Reagents, Cloning Kits and Modifying Enzymes

224 Exhibition ChunLab, Inc. Booth No. 14 천랩 Homepage: CEO: Jongsik Chun Tel.: Fax: Address Bldg 105-1, Suite #307, Seoul National University 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea Main Technology and Products ChunLab is the world's leading specialist in bioinformatics solutions for next generation sequencing in the areas of microbial genomics, metagenomics and transcriptomics. Using our proprietary and breakthrough algorithms and statistical analyses, we have developed intuitive and user-friendly bioinformatic tools that allow our clients to perform cutting-edge research with improvements in speed and depth of analysis at a lowered cost. DNA Link USA, Inc. Booth No. 15 디엔에이링크 Homepage: CEO: Jong Eun Lee office@dnalink.com Tel.: Fax: Address 2F, Cluster Center, Ewha Womans University, 150 Bukahyeon-ro, Seodaemun-gu, Seoul 03759, Korea Main Technology and Products Next Generation Sequencing (NGS) is not only used in a typical bulk sequencing, but also in variety of research areas such as, cancer genomics, RNA sequencing, and Epigenomic analysis. DNA Link possesses diverse NGS platforms (Illumina Hiseq2000/2500, Ion PGM, and Ion Proton) and provides the right experimental design and optimal technology for sequence capture based on different NGS applications. Our PacBio RSⅡ system was the first to launch its service in Asia, and its groundbreaking performance in de novo sequencing promises to cut down research time and cost. DNA Link guarantees an optimal service from the selection of application-oriented platform to the comprehensive Bioinformatics service. Macrogen Inc. Booth No. 16 마크로젠 Homepage: CEO: Hyonyong Chong sales@macrogen.com Tel.: Fax: Address 10F, 254 Beotkkot-ro, Geumcheon-gu, Seoul 08511, Korea Main Technology and Products Macrogen is a leading integrated genomic research service provider dedicated to providing the highest quality genomics services. We provide wide range of sequencing and bioinformatics services to academic, pharmaceutical, and clinical research communities around the world. With over 17 years of experience in genomics, we are dedicated to improving the quality of life for mankind by enhancing the understanding and availability of various genome information. DYNEBIO INC. Booth No. 17 다인바이오 Homepage: CEO: Je Hyeon Lee dyne@dynebio.co.kr Tel.: Fax: Address B-2005, 14 sagimakgol-ro 45beon-gil, Jungwon-gu, Seongnam-s,i Gyeonggi-do 13209, Korea Main Technology and Products Dyne LoadingSTAR is designed for eliminatinig any staining procedure. It contains a sensitive, stable and relatively safe fluorescent dye designed to replace the highly toxic EtBr for staining DNA. DNA bands can be visualized immediately after the run by placing the gels on a standard UV transilluminator

225 2016 International Meeting of the Microbiological Society of Korea BioD Co., Ltd. Booth No. 18 바이오디 Homepage: CEO: Yeojun Nam Tel.: Fax: Address A-1102, Gwangmyeong Technopark, 60, Haan-ro, Gwangmyeong-si, Gyeonggi-do 14322, Korea Main Technology and Products BioD is an expert company of PCR, qpcr and digital PCR. Our products range is amplification reagents, instruments and imaging analysis systems. Chemiluminescence system, Gel Documentation system, PCR, Real-Time PCR, Digital PCR, Automated Nucleic acid Extraction, DNA Fragment Analyzer, PCR Enzymes, Real-time PCR Enzymer, Oligo Synthesis, Gene Synthesis, NGS Service. MegaBio Co., Ltd. Booth No. 19 메가바이오 Homepage: CEO: Bong-deok Han Tel.: Fax: Address #201, 83 Dunsan-daero 117beon-gil, Seo-gu, Daejeon 35203, Korea Main Technology and Products Guava easycyte Flow Cytometers Our microcapillary flow cytometry systems are simpler to operate than traditional sheath-fluid based instruments and are far easier to maintain. They utilize small sample volumes, generate minimal waste, and have lower operating costs. As a result, Guava easycyte flow cytometers are uniquely amenable to on-demand use in the laboratory environment and have helped many scientists achieve insightful cellular analysis since Muse Cell Analyzer The Muse Cell Analyzer delivers accurate assessments of cell health parameters, cell signaling activation pathways & immune status in just minutes, revolutionizing the way you analyze cells. Roche Diagnostics Korea Booth No. 20 한국로슈진단 Homepage: CEO: Richard Yiu korea.lifescience@roche.com Tel.: Fax: Address 4F. Seokyung Bldg., Teheranro 108-gil 22, Gangnam-gu, Seoul 06174, Korea Main Technology and Products Building on over 30 years of industry experience, Roche Custom Biotech uses the powerful multidisciplinary skill found in Roche facilities across the world. The team offers products and solutions for manufacturers in the idagnostics and pharma biotech industry ranging from raw materials to system solutions for bioprocessing. ShinYoung Corporation Booth No. 21 신영코퍼레이션 Homepage: CEO: Yujeng Choi syc@sycos.co.kr;info@sycos.co.kr Tel.: Fax: Address 1F&2F, Kwangmyeong Bldg, #22 Yangjae cheon-ro 21-gil, Seocho-gu, Seoul 06748, Korea Main Technology and Products ShinYoung Corporation is a company that import and supply instruments, plastic consumable, pipette, filter tip, chemical, excipient from STARLAB GmbH, Panreac Applichem, Hilgenberg GmbH, Luzchem. In addition, we are performing the nation s first One-stop pipette service for calibration and repair of all brands pipettes

226 Exhibition Solgent co., Ltd. Booth No. 22 솔젠트 Homepage: CEO: Hyeonkun Myeong, Seongjun Yi Tel.: Fax: Address 3f, 32, Techno 6-ro, Yuseong-Gu, Daejeon 34014, Korea Main Technology and Products Since 2000, SolGent has been manufacturing and supplying various products and services for the purpose of providing total solution for genetics. Our Molecular diagnostics, biology reagents and sequencing services are generated in-house, adhering to the industry's strict quality controls. Business range of SolGent : Molecular diagnostics / molecular biology / genetic analysis service. Beckman Coulter Korea Ltd. Booth No. 23 한국벡크만쿨터주식회사 Homepage: CEO: Kyoung Yong Lee LSKorea@beckman.com Tel.: Fax: Address 3rd Fl., Suseo Bldg., 281, Gwangpyeong ro, Gamnam gu, Seoul 06349, Korea Main Technology and Products Coulter principle (Multisizer 4e) Discover the most versatile and accurate particle sizing and counting instrument on the market, using the Coulter Principle. The Coulter Principle has been used to characterize thousands of different industrial and biological particulate materials. The Multisizer 4e Coulter Counter can be used to count and provide mass distribution for marine biology and microbiology and also the Multisizer 4e detects cell size and volume changes event if they happen over a few seconds or in a period of several hours. Change in cell volume is an important factor involved in many biological processes such as Cell Growth, Cell Cycles, Cell Death, and Compensation for Osmotic Stress, Pathogenesis and Phagocytosis. RAONTECH CO., LTD. Booth No. 24,25 주식회사라온테크 Homepage: CEO: Yeon Ho Kim info@raontechkorea.com Tel.: Fax: Address 2, Uchi-ro 331beon-gil, Buk-gu, Gwangju 61051, Korea Main Technology and Products It is equipment which is essential for analyzing microorganism. We have Bag Mixer, Gravimetric Dilutor, Automatic Dilutor & Plater, Automatic Colony Counter, Anaerobic Workstation, Anaerobic Jar System, etc. Also, we supply the microbiological experiment tools professionally. Korean Collection for Type Cultures (KCTC) Booth No. 26 한국생명공학연구원생물자원센터 Homepage: CEO: Doo-Sang Park sales@kribb.re.kr Tel.: Fax: Address Korean Collection for Type Cultures (KCTC) Korea Research Institute Bioscience and Biotechnology(KRIBB) 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Korea Main Technology and Products - Microorganism : Type strain, Reference strain, Patent strain - Cell lines : Animal cell line, Plant cell line - Identification of Microbe - Package service : Culture extract - Genomic DNA, Proteome for Microorganism - Metagenome library 225

227 2016 International Meeting of the Microbiological Society of Korea GenomicBase Inc. Booth No. 27 제노믹베이스 Homepage: CEO: Chan Do Yun Tel.: Fax: Address 4F,9 Janghan-ro 17gil, Dongdaemun-gu, Seoul 02628, Korea Main Technology and Products Real Time PCR machine/ Auto Prep Machine/ Thermal Cycler LED Transilluminator/ Gel Doc. System/ Homogenizer N3D culture/ Transfection Reagents/ Biochemicals Korea National Microorganisms Research Resource Center(KNMRRC) Booth No. 28 ( 국가연구소재 ) 미생물거점센터 Homepage: CEO: Sangseob Lee sslee@kyonggi.ac.kr Tel.: Fax: Address Research center R201, , Gwanggyosan-Ro, Yeongtong-gu, Suwon-si 16227, Gyeonggi-do Main Technology and Products The Korea National Microbiological Research Resource Center is the core center of the twelve microorganism banks designated by the Ministry of Education, Science and Technology. The KNMRRC supports microorganism banks with necessary guidelines, standards, training for efficient operation of the banks. It also provides with an effective forum to solve common issues of the related banks. The ultimate goal of the KNMRRC is the followings: 1 construction of standardized and integrated management system, 2construction of Core center and other organs network, 3 Quality Control(QC) of microbial resources in the member banks, 4conservation of Resources in the member banks and the interrupted banks, 5education for professionals in the member banks, 6public Relations for raising people's awareness of the importance of microbiological resources. Microbial Institute for Fermentation Industry Booth No. 29 ( 재 ) 발효미생물산업진흥원 Homepage: CEO: Jeong Do-Youn jdy2534@korea.kr Tel.: Fax: Address 61-27, Minsongmaeul-gil, Sunchang-eup, Sunchang-gun, Jeollabuk-do 56048, Korea Main Technology and Products Microbial Institute for Fermentation Industry is aiming to collect, manage and utilize an extensive microbial resources. As a research organization, we are offering an extensive collection of microbial resources, technical services and educational programs to support agricultural, food, pharmaceutical industries. icell Co. Booth No. 30 아이셀 Homepage: CEO: Yong-Chul Lee icellsci@icellsci.com Tel.: Fax: Address 429 ITECO, 150 Jojeong-daero, Hanam-si, Gyeonggi-do, 12930, Korea Main Technology and Products icell is developed the relationships with Merck Millipore, Promega, Helios, Glentham, NEST and other life science services in Korea. We implement about pre-made buffer of DNA, RNA and protein research in our brand, CellNest. icell is a leading company for high quality in life science and biotechnology

228 Exhibition KOMA BIOTECH Booth No. 31 고마바이오텍 Homepage: CEO: Sang-Hoon Moon Tel.: Fax: Address 19F, IS BIZ Tower, Yangpyeong-ro 21 gil 26, Yeongdeungpo-gu, Seoul 07207, Korea Main Technology and Products Electrophoresis system & Pre-cast gels, PCR reagents, Inhibitors, ELISA, Luminex & Multiplex kits, Drug Discovery service, Custom virus generation service, stable cell line, antibody production service, Gene synthesis, Peptide synthesis, etc. WOOSUNG CRYOTECH CO., LTD. Booth No. 32,33 우성크라이어텍 Homepage: CEO: Min Cheol Seok Tel.: Fax: Address #409, B dong Ssangyong, IT Twin 442-5, Sangdaewon-dong, Joongwon-gu, Seongnam-si, Gyeonggi-do 13216, Korea Main Technology and Products We import a LN2 Liquid Nitrogen Tank & Container, VIP, Biological Safety Cabinet, Ultrasonicator, Cryo labeling system and supply these items to hospital, university, institute in Korea. We will do our best to contribute in biomedical field for national development. Sartorius Korea Biotech Co., Ltd. Booth No. 34,35,38,39 싸토리우스코리아바이오텍 Homepage: CEO: DS Kim info@sartorius.co.kr Tel.: Fax: Address 8F Solid Space, Pangyoyeok-Ro 220, Bundang-gu, Seongnam-Si, Gyeonggi-do 13493, Korea Main Technology and Products Sartorius is a leading international pharmaceutical and laboratory equipment supplier. With our innovative products and services, we are helping our customers across the entire globe to implement their complex and quality-critical bio manufacturing and laboratory processes reliably and economically. MGMED, Inc. Booth No. 36 엠지메드 Homepage: CEO: Ho Young Kang, Byung Wha Lee doctorprotein@doctorprotein.com Tel.: Fax: Address 10F Elysia bldg., 173 digital-ro, Geumchun-gu, Seoul 08511, Korea Main Technology and Products Doctor Protein is a specialized brand for diagnostic and enzyme reagents with the novelty and convenience for research. Our Product : Polymerase for PCR / Real-time PCR reagents / Enzymes and Kits for Molecular Biology / Protease and Phosphatase Inhibitor cocktails / Apoptosis Detection Kits / Mycoplasma Detection Kit / Biotool Product 227

229 2016 International Meeting of the Microbiological Society of Korea Hanil Sci-Med Co.,Ltd. Booth No. 37 한일사이메드 Homepage: CEO: Jun Hyeonggon Tel.: Fax: Address 5-4, Songnim-ro 48beon-gil, Yuseong-gu, Daejeon 34098, Korea Main Technology and Products Hanil Sci-Med Co., Ltd is specialized in manufacturing and selling specialized products for research and production in the fields of solid-liquid separation process of chemical, pharmaceutical and biotechnology. Through many years of customer management system, we are recognized for our equipments excellence and follow-ups by our customers and we will do our best in order to satisfy our customers needs with the best solution. Korea Bio-Tech Co., Ltd. Booth No. 40 케이비티 Homepage: CEO: KIM HYEONG KIL Tel.: Fax: Address 5, Dongwon-ro 21-beon gil, Bundang-Gu, Sungnam-Si, Gyeonggi-Do 13547, Korea Main Technology and Products Products: Thermal Cycler, Electrophoresis System, Microplate Reader, Gel Doc., UV/Vis Spectrophotometer, Homogenizer, Tissue Grinder, Speed Vac, Oven, Incubator, Environmental Chamber, Freeze Dryer, Mixer, Stirrer, Peristaltic Pump, etc. Life Science Publishing Co. Booth No. B01 라이프사이언스 Homepage: CEO: Hyo-Joong Kim Tel.: Fax: Address A-303 Halla APT Sangga, 9, Ttukseom-ro 35-gil, Gwangjin, Seoul 05070, Korea Main Technology and Products Our Company is lifescience publishing company. Our company has a good reputation for good books We give a top priority to customer satisfaction. PanMun education Booth No. B02 주식회사범문에듀케이션 Homepage: CEO: Sung-kwon, Liu tshan@epublic.co.kr Tel.: Fax: Address 211, Mokdongseo-ro, Yangcheon-gu, Seoul 07995, Korea Main Technology and Products For 60 years, Panmun Education is dedicated to publishing translations of renowned books and the digital contens on natural science, medicine, phamacology, health and veterinary studies. Furthermore, we have produced and distributed various contents regarding medicine and natural science

230 Author Index

231 2016 International Meeting of the Microbiological Society of Korea A Adedoyin, Gloria D017 Agrawal, Anurodh Shankar S16-2 Ahn, Gna D018 Ahn, Hyeon Yeong S8-4 Ahn, Jae-Hyung A030, A031 Ahn, Jinsook E025 Ahn, Ji-Young D018, G029, G031 Ahn, Joong-hyeon A014, B013 Ahn, Sungeun B024 Ahn, Tae Seok B006 Ahn, Tae-Young A041, A042 Ahn, Woo-Chan E007 Algaissi, Abdullah S16-2 An, Ji Eun E004 An, Jieun H006 Asaf, Sajjad C006 Averette, Anna K D017 B Bae, Jin-Woo A020, A021, A022 B028, B034, C020, D029 D044, H019, S3-1 Bae, Jung Eun G025, G030 Bae, Kyung Sook A033 Bae, Sarah A040 Bae, Seung Seob G043 Bae, So-Hee D004 Bae, Yu-Ra B012 Baek, Je-Hyun D010 Baek, Kiwoon A023, A036 Bahn, Yong-Sun D015, D016, D017 F005, S13-2, YS2-6 Bai, Jaewoo S6-2 Bain, Judith M. S13-1 Bang, Iel Soo E024 Bang, Soohyun D015, D017 Bari, Wasimul A006 Bartlett, Douglas H. S18-3 Beja, Oded PL-2 Beom, Ji Yoon E014 Blanchard, Laurence S4-1 Bok, Jinduck G028 Bong, Ki Moon C003, C004 Bumann, Dirk PL-4 Byun, Hyo-Jeong D017 C Campeotto, Ivan PL-1 Cao, Thinh-Phat D035 Cardenas, Maria E. D055 Cha, Chang-Jun A007, B032, B046 Cha, Kyoung Eun H028 Cha, Soohyun D017 Chae, Jong Pyo S3-5 Chae, Jong-Chan B046, B047, B048 Chae, Suhn-Kee F009 Chae, Tong Un G001, G002, G003, GS-3 Chai, Han-ha C001 Chan, Tehsheng S16-2 Charoenlap, Nisanart S17-4 Chembazhi, Ullas Valiya E006 Cheon, Hyoung Tae B030 Cheong, Eunji D017 Cheong, Hyang Min D030 Chi, Youn Tae G032, G033, G034 G035, G036 Chio, SeongYeol C005 Cho, Ahn Na S15-4 Cho, Chongsu G028 Cho, Ga Youn A012, B043 Cho, Ga Young D013 Cho, Han a A042 Cho, Hansam H017, H018 Cho, Hee-Young G031 Cho, Hyosun D037, D038 Cho, In Sook H001 Cho, Jang-Cheon A026, A027, A028 A029, A034, B030, B040, YS1-4 Cho, Min-Kyung D046 Cho, Miyeon C027 Cho, Nam-Hyuk S12-4 Cho, Se Hyun H013 Cho, Seong Jun C021 Cho, Se-Young H013 Cho, Sung-Jin B021, C014, D018 Cho, Yeondong H017 Cho, Yong-Jun G043 Cho, You-Hee E016, F014 Choe, Mangyu YS2-4 Choi, Ae Ran G043 Choi, Ahyoung A034 Choi, Chang Won D019, D020 Choi, Chi Won D043 Choi, Eun Ha C017 Choi, Eun Yong E004 Choi, Eunna YS2-3 Choi, Gug Seoun H001 Choi, Hak-Jong A003 Choi, Hanul H018 Choi, Hey Young H029 Choi, Hueng-Sik YS2-5 Choi, Hyung Tae E009 Choi, Hyunjun A018 Choi, In-Geol G039, G045, S6-3 Choi, Jaeyoung D017, S7-2 Choi, Ji Eun F010 Choi, Jiwon H018 Choi, Jong-Soon B001, D017, H013 Choi, Jongwoon A004 Choi, Joo Bong H015 Choi, Joon-Sun C002 Choi, Kyeong Rok G012, G013 G014, G015 Choi, Kyoung-Hee S5-2 Choi, Kyung-Hwa S19-2 Choi, Sang Ho E022, E025, F001 F008, GS-4 Choi, Seon A008 Choi, Seong Yeol B011 Choi, SeongYeol C007, C015 Choi, Seung Kook H001 Choi, Sol G002, G003, GS-3 Choi, SooIn D014 Choi, Soomin D006 Choi, Su Yeon B039 Choi, Won Ja E004, E005 Choi, Yong Jun G010, G011, G012 Choi, Yujin A013 Choi, Yunjaie G028 Choi, Yun-Jaie H003, H005 Choy, Hyon E. C019, D003, F019 S14-3, YS

232 Author Index Chun, Byung Hee H009 Chun, Ho Hyun S11-1 Chun, Jongsik A009, S3-3 Chun, Se Chul C017 Chun, Yu Jin D010 Chung, Dawoon F009 Chung, Eu Jin A032 Chung, Han Young F001, F008 Chung, In-Young E016, F014 Chung, Jae Keun D045 Chung, Kyung Min S9-1 Chung, Woo-Hyun F010, S2-1 Chung, Young Bae S11-1 Chung, Young Ryun A032 Corrigan, Rebecca M. PL-1 Couch, Robert B. S16-2 Cui, Heqing E014 D Dananjaya, Sajith D032 Daungnkern, Jintana S17-4 De Groot, Arjan S4-1 Demin, Annie Albert S2-2 Deng, Zixin S18-3 Devarayan, Kesavan B026 Dhanasingh, Immanuel E020 Di, Doris Y. W. B050 Do, Eunsoo F013 Duong, Tham Thi A019 Dwidar, Mohammed D014 E Ehrlich, Rachel L. PL-3 Ekwe, Adaeze A014 Elvebakk, Arve S7-3 Eom, Ahn-Heum B012 Eom, Jeong Seon G019, G020 G021, G022 Eom, Ji Eun H002 Erwig, Lars P. S13-1 Festa, Richard F D015 Filler, Scott G. Floyd, Anna Freemont, Paul G PL-3 D015 PL-1 Gao, Shou-Jiang D039, S12-1 Garron, Tania S16-2 Ghatge, Sunil G047 Gim, Geun Ho B053 Go, Junhyeok GS-6 Goo, Jung Hyun D049 Gründling, Angelika PL-1 Guevarra, Robin B. S3-4 Gwak, Hyun Jung S11-1 Gwak, Joo-Han B037, B044 Gwon, Yong-Dae H017, H018 H Ha, Dong Ryong D048 Ha, Ji Yeon G004, G005 G006, G007 Ha, Jimyeong S5-2 Ha, Nam Chul E021 Ha, Nam-Chul E016, E022, E025 S9-3, YS2-4 Ha, Sanghyun S11-1 Ha, Un-Hwan S5-4 Häggblom, Max M. S10-2 Hahn, Tae-Wook F018 Ham, Junsang C001 Han, Ae Ri S11-1 Han, Ah-reum F006 Han, Dalmuri D002 Han, Dukki B049 Han, Geon Goo H003, H005 Han, Hyo Mi D024 Han, Ji-Hye A013, A023, A036 Han, Sang Eun G025, G030 Han, Sanghyun H004 Han, Seung Hyun D046 Han, Sung Wook C021 Han, Yoontak YS2-3 He, Ying S18-3 Heitman, Joseph D015, D055, S13-1 Heo, Eungyeong B038 Heo, Gang-Joon H011 Heo, Jaehyuck H018 Heo, Jihune F016 Heo, Kyoo E010 Heo, Su Jin C021 Heo, Yoonki H017 Holzapfel, Wilhelm H. S1-2, S1-3 Hong, Chong-Hae D002 Hong, Heeji B035 Hong, Hee-Ji B037 Hong, Hyunjin H034 Hong, Jisoo D008, YS1-1 Hong, Joohyeon D017 Hong, Seung-Beom A030, A031 Hong, Soon Gyu A039, B040, S15-4 S7-1, S7-3 Hong, Soon Ho G016, G017 Hong, Yeongjin C019, S14-3 Hong, Young Ho C021 Hong, Young-Shick S11-2 Hossain, Sabrina H011 Hur, Hor-Gil B049, B050, C026, G047 Hur, Jae-Seoun H032, S7-2, S7-4 Hwang, Chung-Yeon B041 Hwang, Jae-yeon E008 Hwang, Ju Yeon D010 Hwang, Jung Moon A032 Hwang, Kwang Yeon F006 Hwang, Kyuin S15-4, S7-1 Hwang, Nakwon B045, H020 Hwang, Seo Yeon S6-2 Hyun, Dong-Wook A020, A021, A022 B034, D029, D044, H019, S3-1 I Ianiri, Giuseppe D015, D016 Idnurm, Alex D015, D016 Im, Hansol D013, GS-2 Im, Jeongdae YS1-5 Im, Sin-Hyeog S8-3 In, Sujin F010 Islam, Md. Arafat B035, B037, B

233 2016 International Meeting of the Microbiological Society of Korea J Jang, A-Yeung D028 Jang, Bo-Ram S17-2 Jang, Ho-Jin B032 Jang, Hye-Jung F014 Jang, In-Ae B025 Jang, Jae Won G002 Jang, Ja-Young A003 Jang, Jeonghwan B050 Jang, Juyeong D017 Jang, Kyoungwoo G039, G045 Jang, Kyung Ku E025, GS-4 Jang, Sungho S14-2 Jang, Yejin B046, B047 Jang, Yo Han S16-1 Jang, Yu Yeon H018 Jang, Yu-Sin G008, G009 Jang, Yuyeon H017 Jebbar, Mohamed S18-1 Jeon, Che Ok A012, A017, A032 B010, B014, B018, C016, E011 H008, H009, H010, YS1-6 Jeon, Hey Hee C016 Jeon, Hye Hee H010 Jeon, Hyungtaek D039 Jeon, JungHun A038 Jeon, Mee-Hyang F009 Jeon, Sun Jeong A040 Jeon, Sung-Sil C009 Jeon, Taeck J. G040, G041, G042, H012 Jeong, Anna C018 Jeong, Do-Youn G019, G020 G021, G022 Jeong, Eun Kyo G030 Jeong, Eunji D015 Jeong, Haeyoung C023, S6-1, S6-4 Jeong, Hye Im A017 Jeong, HyeIm B010 Jeong, Jae Ho C019 Jeong, Jae-Ho YS2-5 Jeong, Jea-Ho D003 Jeong, Jung Sun G030 Jeong, Sang Eun A017, B014 Jeong, Seong-Yeop G019, G020 G021, G022 Jeong, So Hyang D045 Jeong, Su-Ji G019, G020, G021, G022 Jeong, Sun Wook F011 Jeong, Sun-hwan B019 Jeun, Eunji S8-3 Ji, Chang-Jun S17-1 Ji, Je-Hyun D017 Ji, Sang hye C017 Ji, Seongmi D019, D020 Ji, Yosep S1-2, S1-3 Jia, Baolei E011, H008 Jin, Chan mun D030 Jin, Gwideuk H005 Jin, Gwi-Deuk A005, C012, H003 Jin, Hyun Mi YS1-6 Jin, Jae-Hyung D016 Jin, Shi Ying E008 Jing, Zhang D028 Jo, Chang Hwa F006 Jo, Eojin H033 Jo, Eun-hye D048 Jo, Eun-Kyeong D011 Jo, Eun-Kyoung S3-2 Jo, Ga Yeon C016 Jo, Inseong E016, E022, E025 Jo, Jihoon A018 Jo, Kyubong S4-3 Joh, Kiseong A015, A016 Joo, Gwonsung H018 Joo, In Sun H015 Joo, Minju F015, F016 Joo, Sung-Ho G026, G027 Joo, Yong Seob A010 Joo, Yoo Jin D010 Joung, Yochan A013, A016, A026 A028, A029, B006 Jung, Dawoon B006 Jung, Gyoo Yeol S14-2 Jung, Hae Chang G043 Jung, Hae-Chang G044 Jung, Hee Dong D030 Jung, Jaejoon B004, B017 Jung, Jin A E008 Jung, Kwang-Woo D015, D017 F005, YS2-6 Jung, Kyu Seok Jung, Man-Young Jung, Mi-Ja Jung, Min Young Jung, Myungjoon Jung, Seok-Won Jung, Seunho Jung, Won Hee Jung, Yuna K B009, D008 B035 A021, B034, D029 D044, H019 E012 C022 C027 H027 F013 F022 Ka, Kang-Hyun B012 Kahng, Hyung-Yeel B015, B022 B027, B029 Kang, Boram B005 Kang, Chang-Yuil D049 Kang, Dae-Kyung S3-5 Kang, Do Won A038 Kang, Hee Kyoung D012 Kang, Heeyoung A015, A016 Kang, Hyojeong D037 Kang, Hyojeung C027 Kang, Ilnam A027, A034, YS1-4 Kang, Ji-hee S1-2 Kang, Joo Won A008, H007 Kang, Keunsoo A041 Kang, Na hee D027 Kang, Sangkee G028 Kang, Sang-Mo C006 Kang, Sung Gyun G043, G044 Kang, Woo Kyu H016 Kang, Woo-Kyu S13-4 Kang, Woorim A020, A022 D029, S3-1 Kee, Hye-young D048 Kil, Dong Yong B007 Kim, Ah-Yeong C006 Kim, Bi-o F014 Kim, Bong-Soo S15-2 Kim, Bu Min C001 Kim, Bum Joo D028 Kim, Byoungjin G005 Kim, Byoung-Suhk B

234 Author Index Kim, Cheon Hyeon D030 Kim, Choon-Mee D040, D041, D042 Kim, Da Hyun A008 Kim, Da Jeong G030 Kim, Dae In A010, H007 Kim, Dae-Ghon G031 Kim, Dae-Shin A001 Kim, Dae-Wi B032, B046 Kim, Daeyoung F015, F016, F017 Kim, Do Hak A041 Kim, Dockyu B003, B038 Kim, Dong In G002 Kim, Dong Joon D033 Kim, Dong Min D045 Kim, Dongho F005, YS2-6 Kim, Dong-Min D040, D041, D042 E017, E018, E019 Kim, Dongwook D047 Kim, Dong-Yeo B012 Kim, Don-kyu YS2-5 Kim, Duwoon H013 Kim, Eun Bae A005, B023, C012 H003, H005 Kim, Eun ji E008 Kim, Eun Jung E004, E005 Kim, Eungbin B038, C022 Kim, Eun-Jung F004 Kim, Eun-Sun D048 Kim, Gong Min C004 Kim, Gun-Hwa D043 Kim, Ha Yeon B036 Kim, Han Bok G018 Kim, Haneul A015, A016 Kim, Hansol A041 Kim, Hasun A004 Kim, Hey-Min E013, E026 Kim, Hong-Man F017 Kim, Hwa Young D034, YS2-1 Kim, Hwang Suk D009 Kim, Hyangmi A033 Kim, Hye Ji B020 Kim, Hye Rim C016 Kim, Hye-Jin F002 Kim, Hyeseon H012 Kim, Hyoun Wook C001 Kim, Hyun Ju Kim, Hyun Jung Kim, Hyun Sik C023 B016 A020, A021, A022 D029, D044, S3-1 G013 C019, D003 G025, G030 G028 Kim, Hyun Uk Kim, Hyun-Ju Kim, In Seop Kim, Inseon Kim, Jae Su S1-1 Kim, Jae Won A038 Kim, Jae-Ouk D049 Kim, Jaewon G039 Kim, Jang Hoon H001 Kim, Jeong Yoon H016 Kim, Jeong-Seon A030, A031 Kim, Jeong-Yoon S13-4 Kim, Ji Hee A039 Kim, Ji Yeon S11-3 Kim, Ji-Eun C002 Kim, Jihyun F. B039, B042, B051 B052, F022 Kim, Jin Kyung D011 Kim, Jin Sung G035 Kim, Jin-Hyon S3-2 Kim, Jin-Sik S9-3 Kim, Jisun YS1-3 Kim, Ji-Young S3-2 Kim, Jong Cheol S1-1 Kim, Jong Min C003, C004 Kim, Jong-Geol B037, S18-2 Kim, Jong-Guk B033 Kim, Jong-Myeong S13-4 Kim, Jong-Shik A001 Kim, Jonguk D008, YS1-1 Kim, Joon D009, D010 Kim, Joong-Su D035 Kim, Jueun H021 Kim, June-Eun D004 Kim, Jung A S7-2 Kim, Jung Hoon E023 Kim, Jung-Hoon S17-1 Kim, Jung-Hun S14-1 Kim, Jungman B045, H020, S3-4 Kim, Junsoo F006 Kim, Keun-Sung C008, C010 Kim, Ki Soon D030 Kim, Kiju F018 Kim, Kun-Hee C019, D003 Kim, Kwang Kyu A001 Kim, Kwangsoo C019, D003 Kim, Kyoung Heon S6-3 Kim, Kyung Hyun A032, B010, E011 Kim, Kyung Mo S15-4 Kim, Mi Sun A008, H007 Kim, Min Jung D049 Kim, Min Kyu F011 Kim, Min Kyung D023 Kim, Mincheal H020 Kim, Mincheol B045 Kim, Minjoo B042, S8-4 Kim, Min-Kyeong A013 Kim, Min-Kyu F005 Kim, Min-Sik G043 Kim, Min-Soo A021, B028, B034 D029, H019, S3-1 Kim, Minsun A041 Kim, Minwook F007 Kim, Moon Jong B053 Kim, Myoun Su E008 Kim, Myung Ju F010 Kim, MyungKil H006 Kim, Na-Eun D004 Kim, Nam Kyu H029 Kim, Ok Sun S15-4 Kim, Pil Soo A020, A021, A022 B034, D029, D044, H019, S3-1 Kim, Pyoung Il C003, C004 Kim, Se Woong D009, D010 Kim, Sehyun H017 Kim, Sei Chang D019, D020 Kim, Sejeong S5-2 Kim, Seo Young A010 Kim, Seo Yun G015 Kim, Seon Kyeong D048 Kim, Seon-Hee B015, B027 Kim, Seon-Won S14-1 Kim, Seul I S6-2 Kim, Seung Bum A013, A014, B013 Kim, Seung Il D043 Kim, Si Wouk B

235 2016 International Meeting of the Microbiological Society of Korea Kim, Sihyeon S1-1 Kim, So-Jeong B037, B044 Kim, Sojung D008 Kim, Soo-Jin A030, A031 Kim, Soo-Kyung D005, E002 E003, S5-1 Kim, SooYeon C005, C007 Kim, Sora B009 Kim, Su Yeon E009 Kim, Suhyun A027, A034, B030 Kim, Sun Hee D045 Kim, Sung Soon D030 Kim, Suok-su G013 Kim, Suyeon F008 Kim, Tae Hoon S1-1 Kim, Tae Sun D048 Kim, Tae Sung D011 Kim, Tae Wan G043 Kim, Tae Yong G011, G013 Kim, Tae-Su A013 Kim, Taesung C023 Kim, Taeyup D017 Kim, Ungtae YS1-5 Kim, Wan Kee E004, E005 Kim, Won Jun GS-3 Kim, Won Mun G026, G027 Kim, Yang-Hoon C013, C014, D018 G029, G031 Kim, Yeong Hyeock H016 Kim, Yeong O H013 Kim, Yeong Ouk A009 Kim, Yeon-Ran E013 Kim, Yeun Jeong D030 Kim, Yong Hyun A005 Kim, Yong Jin H035 Kim, Young Bong H017, H018 Kim, Young Ho H001 Kim, Young Ran D056, D057, S9-1 Kim, Young-Chang B048 Kim, You-Tae F012 Ko, Dennis C. S13-1 Ko, Gwang Pyo A043 Ko, Haesu C008, C010 Ko, Su Jin D022 Ko, Suk-Hyung A001 Ko, Yongseok A042 Koh, Hyeon-Woo A002, B002 Koh, Sun-Ki F009 Koh, Young Jin H032 Kong, Changsu H005 Kong, Hyun Gi B039 Koo, Jung Mo D019, D020 Koo, Seoyoun A042 Kook, Joong-Ki H033 Kulatunga, Chanuka D033 Kwak, Jun-Yong F009 Kwak, Min-Jung B039, B042 B051, B052 Kwon, Eun-Kyung S12-4 Kwon, HeeUn C007, C015 Kwon, Hyojeong D017 Kwon, Joseph H013 Kwon, Kae Kyoung A035, G043, S18-4 Kwon, Min-Sung A003 Kwon, Mi-Ra C008, C010 Kwon, Oh Sik G023 Kwon, Soon-Kyeong B052 Kwon, Soon-Wo A030, A031 Kwon, Sun Jung H001 Kwon, YeongMi C007 L LaPointe, Gisèle S8-2 Lee, ARa G041 Lee, Bang Yong B003 Lee, Boeun F015, F016, F017 Lee, Byungho F001, F008 Lee, Changhon S8-3 Lee, Chang-Ro YS2-4 Lee, ChangSeok C007 Lee, Chang-Won A038 Lee, Choong Hwan S1-4 Lee, Chul Won C003, C004 Lee, Chul-Hwan S2-2 Lee, Do-Hoon A007, B032 Lee, Dong-Gi D017 Lee, Dong-Heon B029 Lee, Dong-Woo C023, S1-5 Lee, Eun-Jin GS-5, YS2-3 Lee, Ga Eun A011, G037, G038 Lee, Geon Hyoung A037 Lee, Hae-Won A003 Lee, Hakmi H034 Lee, Hayoung D043 Lee, Hee Jung H015 Lee, Hee-Jung H017 Lee, Hee-Yoon D009 Lee, Hong Kum S15-4 Lee, Hwan Hee D037, D038 Lee, Hyang Burm A019, A040 Lee, Hye-Jeong S13-4 Lee, Hye-Mi D011, S3-2 Lee, HyeonGwon B051 Lee, Hyo Jung B014, B018, H008 Lee, Hyoung Ju B039 Lee, Hyun Sook G043, G044 Lee, Hyung Tae D002 Lee, Imchang A009 Lee, In-Jung C006 Lee, Jae Bong B034 Lee, Jae-Hak G043 Lee, Jaejin B005, YS1-5 Lee, Jang-Won D015 Lee, Jang-Woo GS-5 Lee, Jeong Su H015 Lee, Ji Hee A008, A010, H007 Lee, Ji Young E014 Lee, Jidam B052 Lee, Jieun A003 Lee, Ji-Hoon S10-3 Lee, Jihyun F010 Lee, Jin Won E023, G046 Lee, Jina H019 Lee, JinHyeng C007 Lee, Jin-Sung C008, C010 Lee, Jin-Won S17-1 Lee, Jin-Woo S18-4 Lee, Jinyoung H005 Lee, Jisu D039 Lee, Jong Hee A003 Lee, Jong Ho S8-4 Lee, Jong-Hee E012 Lee, Jong-Kook D026 Lee, Joone-Hee E

236 Author Index Lee, Joong-Bok H018 Lee, Joon-Hee D005, E002, S5-1 Lee, Joungmin G009, G015 Lee, Ju-Hoon F012, S8-1 Lee, June Bong D002 Lee, June-Young A020, A021, A022 D029, S3-1 Lee, Jung Yoon D045 Lee, Jung-Hyun G043, G044, S18-4 Lee, Jung-Shin GS-8, H021, H022 H023 Lee, Jung-Sook A001 Lee, Jun-Yeong H003, H005 Lee, Kang-Mu GS-6, S5-3 Lee, Kangseok F015, F016, F017 Lee, Keehoon GS-6 Lee, Keon Jin S11-1 Lee, Keun Chul A001 Lee, Ki-Sung D019, D020, G026, G027 Lee, Ko-Eun C006 Lee, Kwang-Su G026, G027 Lee, Kyeong-Ah C014, D018, G031 Lee, Kyeoung-Ah C013 Lee, Kyu Chan B007 Lee, Kyu-Chan S2-4 Lee, Kyu-Ho S17-2 Lee, Kyung-Jo S17-2 Lee, Kyung-Tae D017 Lee, Kyung-Tai C008, C010 Lee, Mi Rong S1-1 Lee, Mi-Hwa A023, A036 Lee, Miju S2-2 Lee, Minho F017 Lee, Min-Ho E007 Lee, Minyoung H034 Lee, Myung-Shin D039 Lee, Na Ryeong E008 Lee, Saeyoung G039, S6-3 Lee, Sang Il S11-1 Lee, Sang Jun C023 Lee, Sang Mok H003 Lee, Sang Seob H024, H035 Lee, Sang Yup G001, G002, G003 G004, G005, G006, G007, G008 G009, G010, G011, G012, G013 G014, G015, GS-3 Lee, Sang-Hee G029 Lee, Sang-Hoon S10-4 Lee, Sang-Jae C023 Lee, Sang-Seob B019, B020, B021 Lee, Sang-Yeop D043 Lee, Se Hee E012, G031, YS1-2 Lee, Se Jin S1-1 Lee, Seo Hee A040 Lee, Seobihn B043 Lee, Seong Hyuk G043, G044 Lee, Seon-Woo B039 Lee, Seulki D037, D038 Lee, Seung Goo B008 Lee, Seung Hwan G012 Lee, Seung Jae G024 Lee, Seung Young D049 Lee, Seung-Joon D047 Lee, Si Young D001 Lee, So Yeon D001 Lee, Soo Chan S13-1 Lee, Soomin C027 Lee, So-Young S3-2 Lee, Sung Haeng D035, E020 Lee, Sung-Jae F007 Lee, Sung-Joon H015 Lee, Sung-Mok G043 Lee, Sung-Yoep D007 Lee, Su-Yeon H006 Lee, Tae Kwon B005, S10-1 Lee, Yang Soo B026 Lee, Ye Ji E004, E005 Lee, Yeh Eun E023, G046 Lee, Yeonhee H034 Lee, Yeonseon D015 Lee, Yin-Won S13-3 Lee, Yong Seok D045 Lee, Yong-Hwan D017, H025, S7-2 Lee, Yoo Kyung B003 Lee, Young Mi E004, E005 Lee, Yun Hee A017 Lee, Yung Mi A043, S15-4 Lee, Yunkyeong A004 Lee, Zee-Won GS-4 Lei, Zhao D028 Lemaitre, Bruno PL-5 Li, Hui S18-3 Li, Shinan D003 Li, Shinnam C019 Li, Xi-Hui D005, E002, E003, S5-1 Liang, Jingdan S18-3 Lim, Daejin C019, D003 Lim, Hyun Soo S15-4 Lim, Jeong-A B009, D008, YS1-1 Lim, Ji Won C023 Lim, Jin A C024 Lim, Ju Young D056, D057 Lim, Sangyong F005, F011, S4-2 YS2-6 Lim, Seouk Hyeon D030 Lim, Seul Ki A003 Lim, Yeonjung A028, B030 Lim, Young Woon B043, GS-1 H002, H029 Lim, Yun Kong H033 Lin, Shunmei D028, GS-7 Löffler, Frank E. YS1-5 Louw, Johanna S13-1 M Madsen, Eugene L. B035 Maeng, Pil Jae F021 Maruthamuthu, Murali kannan G017 Meyers, Gena Lee D017 Min, Jiho C013, C014, D018 G029, G031 Min, Jung-Joon S14-3 Mitchell, Aaron P. PL-3 Mitchell, Robert D013 Mitchell, Robert J. B011, C005 C007, C011, D014 Mitchell, Robert James C015 Mohammed, Mohammed D036 Mongkolsuk, Skorn S17-4 Monnappa, Ajay D013 Monnappa, Ajay K. A006 Moon, Eun Pyo E004 Moon, Yuseok D047 Mun, Wonsic B011 Myeong, Nu Ri F

237 2016 International Meeting of the Microbiological Society of Korea Myoung, Jinjong Myung, Heejoon D050, D051 D052, D053, D054 H028 N Na, Do Kyun G007 Na, Dokyun G004, G005 Na, Eun Jung F001, F008 Na, Sang Hoon G034, G036 Na, Soo Hui E021 Nai, Yu-Shin S1-1 Nam, Doo Hyun F020 Nam, Gi Gyun A026, A029 Nam, Ki Hyun F006 Nam, Seung-Il B049 Nam, Young-Do S11-4 Nam-gung, Byeol E001 Nasutution, Olviyani E005 Neyrolles, Olivier S9-4 Nguyen, Linh Trieu Dieu E009 Nguyen, Phat-Loc C013 Nguyen, Quang-Thai C014 Nguyen, Son G. B045, H020 Nguyen, Thi Thuong Thuong A040 No, Ji Hyun S10-1 No, Keung Eu D030 Noh, Han Byul D019, D020 Noh, Hyun Joo S15-4 Noh, Hyun-Ju B040 Noh, Youran D049 O Oh, Chang-Kyu D047 Oh, Dong-Chan D011 Oh, Hyeon Song G032 Oh, Hyun-Woo A033 Oh, Jeong-Il S17-3 Oh, Jeongsu S15-4 Oh, Jungmin D005, E002 Oh, Jun-Soo H023 Oh, Kye-Heon E017, E018, E019 Oh, Mi-Hwa C001 Oh, Sejong C018, C024, C025 Oh, Seung-Yoon B043, GS-1 Oh, Sung Oh, Young Joon Oh, Young Taek P D019, D020 A003 D034, YS2-1 Padakandla, Shalem Raj B046, B047 Pajarillo, Edward Alain B. S3-5 Pak, Jae In A005 Pan, Jae-Goo S6-1 Park, Ae Ran S13-3 Park, Bae Keun D033 Park, Bohyun F010 Park, Boyeon E012 Park, Byeong Hyeok S6-3 Park, Byeonggyu G040 Park, Byung-Chul S1-6 Park, Chae Haeng S7-1, S7-3 Park, Chulwoo B017 Park, Chungoo A018 Park, Doo-Sang A033 Park, Doyoung B031 Park, Duck Woong D045 Park, Edmond Changkyun D043 Park, Eunji D021 Park, Goun D017 Park, Gyungsoon C009, C017 Park, Ha Ju B003 Park, Hae Woong A003, E012, S11-1 Park, Haju B038 Park, Hee-Deung S10-4 Park, Hee-Soo D055, F021 Park, Hongjae G039, G045 Park, Hye Jung D045 Park, Hye Min G014 Park, Hyegwon G005 Park, Hyeok B012 Park, Hyun B003 Park, Hyun Jung D019, D020 Park, In Cheol C003 Park, Jae Young H002, H029 Park, Jameon G018 Park, Jeong-Hoon S10-4 Park, Ji-Bin S14-1 Park, Jin Hwan G011 Park, Jin Sook A011, G037, G038 Park, Jin-Woo B009, D008 H004, YS1-1 Park, Jong Gwan D025 Park, Jong Soo S15-3 Park, Jongbin A005, C012, H005 Park, Joon-Woo H011 Park, Ju-Eon S14-1 Park, Jun Seok G013 Park, Jung Wook D045 Park, Ki Hoon H017 Park, Kwang-hyun B008 Park, Kyeong il C019 Park, Kyeongil D003 Park, Min Ju C021 Park, Miri A029 Park, Mi-Rim C008, C010 Park, Myung Soo B043, H002 Park, Nohra E022 Park, Ok-Jin D046 Park, Sam Yong F006 Park, Sang-Cheol A009 Park, Sang-Kook E017, E018, E019 Park, Se-Ho F013 Park, Seok Hyun G013 Park, Seon Young G008, G009 G010, G011 Park, Seong-Hwan D047 Park, Seung Won C021 Park, Seung-Hwan S14-3, S6-1 Park, Shinae GS-8 Park, Si Jae G012, GS-3 Park, Soo-Je A002, B002, B037, B044 Park, Sook-Young S7-2 Park, Soon-Nang H033 Park, Soyoung S1-3 Park, Suhk Hwan A037 Park, Sung Woo D017 Park, Sunhwa C026 Park, Sunjoo A033 Park, Woojun B004, B016, B017 B024, B025, E015, YS1-3 Park, Yeon-Gyeong C006 Park, Yoon Kyung D025 Park, Yoon Mee E

238 Author Index Park, Yoonkyung D012, D021, D022 D023, D024, D026, D027 Park, Young Kwang D009, D010 Park, Young-Ha E010, E013 E026, YS2-4 Park, Young-Min D017 Pathiraja, Duleepa S6-3 Patil, Vinod Vikas B008, E006 Pau, Biak Sang E006 Peng, Bi-Hung S16-2 Pengkit, Anchalee C009 Pham, Van Dung G016 Philippot, Laurent B004 Pignol, David S4-1 Pyo, Suhkneung D007 Qian, Zhi-Gang Q R G014 Rani, Sudas A002 Reddy, Motakatla Venkateswer B048 Rhee, Dong Kwon D007 Rhee, Joon Haeng S14-3, S9-1 Rhee, Sung-Keun B035, B037 B044, S18-2 Rho, Semi D049 Rittiroongrad, Surawach S17-4 Roe, Jung-Hye C002, F003, F004 Roh, Eunjung D008, YS1-1 Roh, Seong Woon A003, B001 Ryu, Jae-Chan GS-6 Ryu, Jae-Gee B009, D008, H004 YS1-1 Ryu, Ji-Hwan GS-6 Ryu, Ki Hyun H031 Ryu, Su Hyun E023, G046 Ryu, Wang-Shick S12-2 S Sanchez, Diana Soils Sandrine, Soh M. H015 C011 Sang, Pau Biak E007 Satchell, Karla J.F. S9-2 Sathishkumar, Yesupatham B026 Schuster, Christopher F. PL-1 Sekhon, Simranjeet Singh C013 D018, G031 Sekhon, Sinranjeet Singh C014 Seo, Boram A043 Seo, Chang-Woo C006 Seo, Eun-Young B006 Seo, Ho Seong D028, S4-4 Seo, Hyun-Seok A035 Seo, Jaegu C022 Seo, Jeong-Ah B042 Seo, Kye Won D048 Seo, Mi Hee D045 Seo, Ra Min G033 Seo, Seung Beom D032 Seo, Sojin F015, F016 Seo, Yeon-Soo S2-2 Seo, Youngdae F003 Seok, Soon-Ja A030, A031 Seok, Yeong-Jae E010, E013 E026, YS2-4 Seol, Eun Taek G024 Seong, Baik Lin S16-1 Seong, Chi Nam A008, A010 A035, H007 Seong, Ho-Jun S3-1 Seong, Hoon Je F002 Seong, Hoon-Je B041 Shahzad, Raheem C006 Shim, Seung-Cheol S3-2 Shin, Bora E015 Shin, Dong-Yeop G042 Shin, Dongyong A004 Shin, Eui-Cheol S12-3 Shin, Eunju H034 Shin, Hea Luying E014 Shin, Jae Ho C009 Shin, Ji Hyun D048 Shin, Joohyun C022 Shin, Minsang F019 Shin, Miyoung C025 Shin, Na-Ri A020, A021, A022, B034 C020, D029, D044, H019, S3-1 Shin, Sang Yeop D033 Shin, Seyeon B022, B029 Shin, Teak Soo S1-1 Shin, Woo-Ri G029, G031 Shin, Yern-Hyerk D011 So, Jae Eun A039 So, Mi Jin H029 So, Yee-Seul D016, D017 Sohn, Jung Hoon S6-1 Sohn, Sung-Oh E012 Solis, Norma V. PL-3 Somasundaram, Sivachandiran G016 Son, Nguyen G. S3-4 Son, Soo Ji C009 Song, Bong Keun G012 Song, Chan Woo G001, G002, G006 Song, Ho Min G034 Song, Hyeyeon S11-1 Song, Hyun Jae D045 Song, Hyunjung S7-2 Song, In-Ja S19-1 Song, Jae Kyeong C004 Song, Ju Yeon B039, B042 Song, Ki-Joon H030 Song, Man Ki D049 Song, ManKi S16-4 Song, Min Young G024 Song, Min-Hee D015 Song, Saemee E022 Song, Seokcheon C022 Song, Seung-Taek S3-2 Song, Sooyeon C018 Song, Woo Sun H016 Song, Wooseok F017 Song, Yoon-Jae D004 Sul, Woo Jun B007, B046, F002, S2-4 Sul, Woo-Jun B041 Sundas, Rani B002 Sung, Hoonje S2-4 Susmita, Nishu S10-1 T Tak, Euon Jung A022 Tao, Xinrong

239 2016 International Meeting of the Microbiological Society of Korea Thak, Eun-Jeong S3-1 Thiele, Dennis D015 Thuy Do, Linh G045 Tiwari, Ravi E006 Tosi, Tommaso PL-1 Tseng, Chien-Te K. S16-2 U Udayangani, Chathurica D032 Um, Si-Hyeon YS2-4 Um, Soohyun D011 Unno, Tatsuya B045, H020, S3-4 Urbanczyk, Henryk S15-1 V Varshney, Umesh E006, E007, S2-3 Vattanaviboon, Paiboon S17-4 Verma, Ravi S8-3 Vinod, Nagarajan D019, D020 W Wang, Chonglong S14-1 Wang, Li Li D017 Wang, Zhijun S18-3 Wee, Gui Nam S10-1 Wendt, Mitchell H011 Weon, Hang-Yeon A030, A031 Whon, Tae Woong A020, A021 B034, C020, D029, D044, H019 Wimalasena, Sudu Hakuruge Madusha Pramud H011 Won, Hong-Hee B041 Woo, Eui-Jeon B008, E006, E007 Woolford, Carol A. PL-3 X Xia, Xiao-Xia G007 Xiao, Xiang S18-3 Xiong, Lei S18-3 Xu, Guanpeng S18-3 Xu, Wenjie PL-3 Yang, Dong-Hoon Yang, Hee Jin Yang, Hee-Jong Y D015, D017 F022 G019, G020 G021, G022 Yang, Jina S14-2 Yang, Jung Eun G012 Yang, Seung-Hoon A001 Yang, Seung-Jo A028, YS1-4 Yang, Suin B009 Yang, Sung-Hyun A035 Yang, Tae-Jun G043 Yang, Yan S18-3 Yang, Yi-Ting S1-1 Yang, Yoon Mo E023, G046 Yang, Youri G047 Yeom, Ji-Hyun F015, F016, F017 Yeom, Se Joung H024 Yeom, Soojin H022 Yi, Dae Gwan F010 Yoo, In-Seol S3-2 Yoo, Jin-Wook E025 Yoo, Ju Eun A043 Yoo, Seung Min G004 Yoo, Seung-min D039 Yoo, Young Ji YS2-2 Yoon, Chang-Kyu E013, E026 Yoon, Hong-sup S1-2 Yoon, Hwang Jae F020 Yoon, Hyunjin S6-2 Yoon, Jang Won D002 Yoon, Jong Kwang H017 Yoon, Ju Yeon H001 Yoon, Jung-Hoon H026 Yoon, Ki Young B042 Yoon, Na-Ra D040 Yoon, Sang Sun D034, D036 GS-6, S5-3, YS2-1 Yoon, Suk Hwan B031, B036 Yoon, Sung Ho S6-4 Yoon, Sung-il E001 Yoon, Yeo Joon E008, E014 S14-4, YS2-2 Yoon, Yeo Kyoung H025 Yoon, Yohan S5-2 You, Young-Hyun B033 Youn, Hyewon S16-3 Yu, Dong Joo G030 Yu, Dong Su H014 Yu, Jae-Hyuk F021 Yu, Woon-Jong B037, B044 Yun, Cheol-Heui D046, S1-6 Yun, Ji-Hyun A021, B034, H019 Yun, Sujin H004 Yun, Sung Ho D043 Z Zhang, Jing F011 Zhao, Lei F011 Zhao, Xu E014 Zheng, Jin Hai S14-3 Zhi, Yong D028 Zoysa, Mahanama De D032, D033 권영광김현김다솔김동건김동연김민석김민진김보람김성현김소윤김연진김영현김윤정김지윤김하연김현아 나지윤남윤성노예림 ㄱ ㄴ HS-15 HS-05 HS-07 HS-28 HS-26 HS-08 HS-28 HS-23 HS-22 HS-25 HS-28 HS-27 HS-04 HS-07 HS-06 HS-06 HS-11 HS-10 HS

240 Author Index ㄹ류혜지 HS-07 ㅂ박상윤 HS-01 박시현 HS-08 박연지 HS-23 박재희 HS-08 ㅅ성지환 HS-16 송종민 HS-17 ㅇ오재석 HS-25 우재연 HS-26 유상훈 HS-27 윤도현 HS-08 윤소희 HS-17 윤이성 HS-18 이민행 HS-27 이상원 HS-16 이수민 HS-04 이승은 HS-02 이승준 HS-15 이용준 HS-20 이재연 HS-24 이정아 HS-19 이주예 HS-23 이준석 HS-16 이준희 HS-15 이지수 HS-19 이창호 HS-16 이현탁 HS-21 임재웅 HS-07 ㅈ전유진 HS-13 정진운 HS-09 정현지 HS-17 조서원 HS-14 조수민 HS-22 조옥현 HS-27 ㅊ천성우 HS-13 천수범 HS-13 천승재 HS-03 최민기 HS-13 최지훈 HS-15 최현빈 HS-24 최혜민 HS-12 최희승 HS-23 ㅎ한종욱 HS-21 홍승희 HS-12

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