Identified and characterized strains which are potential candidates to use as microbial inoculants for sustainable agriculture
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- Leonard Hicks
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1 Ientifie an characterize strains which are potential caniates to use as microbial inoculants for sustainable agriculture
2 Have a private l strain collection of approx iate as enophytes of rice. The following strains have been characterize for their lant Growth romotion otential in vitro an in vivo
3 Ien&fica&on: Microbacterium testaceum Origin: Iate from sterilize rice stem of the Vialone nano variety (Vercelli 2011) Enophy&c bacterium (3.5x10 3 cfu/gr) In vitro G: rotease Lipase ES Movement In planta G: +16% of ry weight (on 40 ays ol rice plants) +11% of ry weight (on 40 ays ol tomato plants) Notes: The genome of AG157 has been sequence by our lab an the sequence is available. The genome isplays highest homology with the genome of Microbacterium testaceum StLB037 iate from potato leaves. Bacteria belonging to this group are oxen iate from the environment an they are recognize as poten&al biocontrol agents.
4 Ien&fica&on: Brevibacillus sp Origin: Iate from sterilize rice stem of the Vialone nano variety (Vercelli 2011) Enophy&c bacterium (7.5x10 3 cfu/gr) In vitro G: rotease Lipase ES Movement In planta G: +8% of ry weight (on 40 ays ol rice plants) +72% of ry weight (on 40 ays ol tomato plants) Notes: The genome sequence of AG162 is currently being etermine. Bacterial strains from this group are recognize to be poten&al biocontrol agents in ifferent plant species an to have a high bioremeia&on poten&al.
5 Ien&fica&on: Bacillus megaterium Origin : Iate from sterilize rice stem of the Vialone nano variety (Vercelli 2011) Features : Rhizosphere bacterium In vitro G rotease Lipase ES Movement In planta G:: +8% of ry weight (on 40 ays ol rice plants) +19% of ry weight (on 40 ays ol tomato plants) Notes: The genome of this microorganism has been sequence by our lab an the sequence is available. Bacillus megaterium is a well stuie species known for its G poten&al through ifferent mechanisms.
6 Ien&fica&on: Herbaspirillum sp. Origin: Iate from sterilize rice roots of the Vialone nano variety (Vercelli 2011) Enophytc bacterium (1x10 3 cfu/gr) In vitro G: rotease Lipase ES Movement In planta G: +36% of ry weight (on 40 ays ol rice plants) +18% of ry weight (on 40 ays ol tomato plants) Notes: The genome of AG215 has been sequence by our lab an the sequence is available. The analysis of the genome sequence has confirme its high homology to Herbaspirillum seropeicae strain SmR which is an enophy&c bacterium recognise for G poten&al ifferent mechanisms.
7 Ien&fica&on: Kosakonia sp Origin: Iate from sterilize rice roots of the Vialone nano variety (Vercelli 2011) Enophy&c bacterium (4.6x10 3 cfu/gr) In vitro G: rotease Lipase ES Movement In planta G: +16% of ry weight (on 40 ays ol rice plants) +14% of ry weight (on 40 ays ol tomato plants) Note: The genome of AG774 has been sequence by our lab an the sequence is available. The genome of strain AG774 is very homologous to Kosakonia sacchari S1T, sequence number C , iate as an enophyte from sugar cane. Bacterial strains of this group are recognize as poten&ally G poten&al through ifferent mechanisms.
8 Ien&fica&on: Curtobacterium sp Origin: Iate from sterilize rice stems of the Vialone nano variety (Vercelli 2011) Enophy&c bacterium (3.5x10 3 cfu/gr) In vitro G: rotease Lipase ES Movement In planta G: +25% of ry weight (on 40 ays ol rice plants) +25% of ry weight (on 40 ays ol tomato plants) Notes: The genome sequence of AG1037 is currently being etermine. The genus Curtobacterium belongs to the family Microceae an inclues a wie range of iate from ifferent environments incluing plant enosphere an rhizosphere. This group has been evience by members being poten&al biocontrol agent inucing the plant systemic resistance (ISR).
9 Ien&fica&on: seuomonas sp. Origin: Iate from sterilize rice roots of the Vialone nano variety (Vercelli 2011) Rhizospheric bacterium In vitro G: rotease Lipase ES Movement In vivo G: +8% of ry weight (on 40 ays ol rice plants) Notes: The genome AG289 has been sequence by our lab an the sequence is available. AG289 belongs to the group of seuomonas fluorescens an seuomonas chlororaphis. The genome of AG289 is highly homologous to seuomonas sp. Os17 an seuomonas sp St29, iate from rice an potato plants an recognize as poten&al biocontrol agents.
10 Legen for in vitro tests: N2: Nitrogen a&on : ACC eaminase ac&vity - involve in ethylen (plant stress hormone) regula&on- : hosphate ubilza&on : inole- 3- Ace&c Aci prouc&on (growth plant hormone) An& : an&fungal ac&vity teste against yricularia oryzae An&bact: an&l ac&vity teste against Dickeya zeae e seuomonas fuscovaginae rotease: proteoly&c ac&vity Lipase: lipoly&c ac&vity ES: Exopolysaccharie matrix secre&on Movement: Swimming an/or swarming movement
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