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1 Syringe Pumps Microfluidic Pumps! We are proud to offer select Harvard Apparatus syringe pumps for use with SynVivo biochips. These high quality pumps have been extensively tested and offer a feature rich solution at an affordable price. Pump 11 Elite & Nanomite Programmable The Pump 11 Elite Series of syringe pumps expands its capabilities to satisfy your experimental requirements. These compact syringe pumps carry on the tradition as the premiere workhorse infusion pump for SynVivo biochip assays, offering unparalleled ease of use with a high resolution touch screen with intuitive icon interface. The Pump 11 Elite Series allows you to create, save and run simple to complex protocols without a PC. The Method profiles available on this pump are Constant Rate, Ramp, Gradient (binary) and Autofill. Delays can be set using the relative time clock. PHD ULTRA Programmable The PHD ULTRA is the solution for your most demanding fluidics applications. This pump represents the latest technology in syringe pumps and was developed utilizing the feedback of the world s largest population of syringe pump users. The PHD ULTRA will change the way you think about syringe pumps In addition, a multitude of syringe racks are available to hold 2, 4 or up to 10 syringes. A microliter syringe rack is also available for microliter applications. Remote versions are also available where the control box and pumping mechanism are separated by a 9.1 meter (30 foot) cable. Please contact us for help in selecting the best pump for your application and a free quote. We are an authorized reseller for Harvard Apparatus Contact us today for a competitive quote: info@synvivobio.com

2 Stagetop Incubators Custom Designed for SynVivo! We have partnered with In Vivo Scientific to develop and sell stagetop incubators specifically for use with SynVivo biochips. For short term or long term studies of living cell cultures under a microscope or for time lapse video microscopy, a stagetop incubator is essential. Perfect for Live Cell Imaging, STEV (the stagetop environmental control system) is a compact environmental case that houses your cell culture s under perfusion and fits on a microscope stage. This system offers precision control of temperature, carbon dioxide, oxygen, humidity as well as remote control and data logging via a USB connection. The system includes the Environmental Control Unit electronics which use four programmable loops to control the temperatures of the water bath and the lid, CO2 and O2 within the environmental case and airflow within the incubator. It comes standard with a digital flow meter for extremely precise delivery of gases, and will work with SynVivo biochips as well as any dishes from 96 wells to 35mm and fits almost all automated stages as well as adapters for manual stages. Please contact us for help in selecting the best stagetop incubator configuration for your application and a free quote. We are an authorized reseller for InVivo Scientific Contact us today for a competitive quote: infoo@synvivobio.com

3 Nikon Instruments An Outstanding Platform! We endorse and distribute Nikon microscope systems and accessories. These are the same scopes we use everyday and we have found them to be an outstanding platform for visualization using SynVivo biochips and assays. Nikon is a world-renowned brand, firmly established as a market leader in optical instrumentation and the only microscope company to manufacture its own glass, ensuring the very finest quality assurance throughout production. With over 90 years expertise in the field, Nikon has always been at the forefront of optical and technological innovation, promoting creativity and trustworthiness as part of the company's global mission statement. Eclipse Ti-U / Ti-E PFS Microscope Systems Scientists have overcome many live cell imaging challenges using advanced techniques such as confocal, TIRF, FRET, photoactivation and microinjection. At the center of it all is the Eclipse Ti, a powerful inverted microscope system that provides instant access to all of these techniques plus revolutionary Nikon CFI60 optics. Available in two models, the Eclipse microscope Ti series offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fullyintegrated microscope system that is ideal for high-end research and live cell imaging. Total System Integration The Ti has multiple configurations that enable many cutting-edge applications, such as TIRF, photoactivation experiments, and much more. Built-in Perfect Focus System The Ti gives you the option of incorporating Nikon's Perfect Focus System (PFS), which provides real-time focus correction during time-lapse observation. Easy Access Controls With operation buttons on both the sides and front of the stand, various controls such as PFS, FL cube, FL shutter, objective mag and image port can be operated quickly and easily. Joystick Control The newly developed joystick and ergonomic controllers allow a custom programmed speed adjustment with a precise and natural feel. Multiple Camera Port The Ti offers multiple camera ports, which can be outfitted with the Digital Sight series high-performance CCD cameras for simultaneous image capture. NIS-Elements Imaging Software The Ti's NIS-Elements software seamlessly integrates the microscope, cameras, components and peripherals, for fully automated image acquisition. We are an authorized reseller of Nikon Instruments Contact us today for a competitive quote: info@synvivobio.com

4 The only in vitro blood-brain barrier model with: The Most Realistic in vitro Blood-Brain Barrier Model Accurate in vivo hemodynamic shear stress Real-time visualization of cellular and barrier functionality Significant reduction in cost and time Robust and easy to use protocols Endothelial Cells Side-by-side architecture enables real-time visualization Basolateral Chamber Apical Chamber Astrocytes + Pericytes + Neurons Physiological dimensions with engineered porous gap Microfluidic platform with ultra-low sample volumes Realistic co-culture with real-time monitoring of cell-cell interactions between vascular, immune, and neuronal cells A highly versatile platform for investigation of: Tight junction proteins Transporter proteins Drug permeability Inflammation Cell migration Omic changes Neurotoxicity Neuro-oncology SynVivo is a physiological, cell-based microchip platform that provides a morphologically and biologically realistic micro-environment allowing real-time study of cellular behavior, drug delivery and drug discovery. Contact Us Today to Discuss Your Research Needs! info@synvivobio.com

5 SynVivo is a physiological, cell-based microchip platform that provides a morphologically and biologically realistic micro-environment allowing real-time study of cellular behavior, drug delivery and drug discovery. A microfluidic cell-based assay platform imprinted with real microvasculature morphology Realistic. Dynamic. Cell-Based Assays. cultured with cells and studied under physiological flow to provide a more realistic in vitro assay. Use Microvascular Networks to replicate in vivo cell/particle adhesion and cell-cell or cell-particle interactions in an in vitro setting. Investigate effects of flow and morphology for drug development and cellular research. Obtain shear-adhesion maps and bifurcation vs. branch adhesion in single experiment. By incorporating natural tissue regions within the network topology, the co-culture networks allow study of cell and drug behavior at and across the interface between the vessel lumen and tissue space. The coculture network constructs are available with several options for channel size, tissue region scaffolding, and barrier design. Intended to mimic the formation of and transport across tight and gap junctions such as the blood-brain barrier and other endothelial/tissue interfaces, the idealized co-culture constructs are available with several options for channel size, tissue chamber size and scaffolding, and barrier design. Contact Us Today to Discuss Your Research Needs! info@synvivobio.com

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