ISCT Paris April 25. Ex vivo platelet production Dominique Baruch INSERM UMR-S1140, Paris
|
|
|
- Angel Gallagher
- 6 years ago
- Views:
Transcription
1 ISCT Paris April 25 Ex vivo platelet production Dominique Baruch INSERM UMR-S1140, Paris
2 Platelet functions: to stop bleeding and beyond Adhesion Aggregation Clot retraction Secretion Procoagulant activity Microparticules «Additional platelet functions continue to come to light, and biologists have just described a novel way that the body might make these multitalented cells a finding that could one day ease the demand for donated blood.» Angiogenesis Source of growth factors Inflammation Immunity Sepsis Metastasis Post-natal vascular remodelling D.Baruch Platelet formation ISCT 25/04/2014 2
3 In vivo platelet production from megakaryocytes Science (2007) Migration of a mouse EYFP- IIb megakaryocyte near a sinusoid Passage of large MK fragments in the circulation 50 m Peripheral venous blood proplatelets D.Baruch Platelet formation ISCT 25/04/2014 3
4 Role of VWF and shear rates on platelet production To address the role of blood flow on megakaryocyte fragmentation into platelets using a VWF-coated surface Perfusion of human mature megakaryocytes in a parallel-plate flow chamber at high shear rates Videomicroscopy recording of single cell trajectories for 30 mins Human megakaryocytes 13 days culture D.Baruch Platelet formation ISCT 25/04/2014 4
5 Proplatelet and platelet formation from human megakaryocytes at high shear rates Flow D.Baruch Platelet formation ISCT 25/04/2014 5
6 Role of microtubules in proplatelet formation 10 images/s Flow D.Baruch Platelet formation ISCT 25/04/2014 6
7 Shear-derived platelets can be activated by thrombin No thrombin Thrombin D.Baruch Platelet formation ISCT 25/04/2014 7
8 Platelet production from mouse megakaryocytes Bioflux mvwf D.Baruch Platelet formation ISCT 25/04/2014 8
9 Shear-induced mouse platelets are functional in vivo in a thrombosis model Topical lesion by FeCl3 on mesenteric vessels Infusion of labelled platelets Intra-vital microscopy : real-time platelet thrombus formation Shear-derived platelets incorporate in thrombus of mesenteric vessels Static conditions or BSA do not support platelet thrombus formation D.Baruch Platelet formation ISCT 25/04/2014 9
10 Is platelet production regulated by Willebrand factor? Transition in circulating blood between different fragmentations states : from preplatelets to proplatelets and to platelets VWF? Adapted from Thon JN et al, JBC 2010 What would be the role of VWF in terminal platelet production? D.Baruch Platelet formation ISCT 25/04/
11 % proplatelets Terminal platelet production in Willebrand factor deficiency Role of exposure of platelets to VWF during their formation Experiments with human VWF deficient platelets of a patient with severe von Willebrand disease :absence of VWF in plasma, platelets, and endothelial cells A pre post C severe VWD B pre post Control Poirault-Chassac & al. PLOS One 2013 D.Baruch Platelet formation ISCT 25/04/
12 VWF Knock out mouse model proplatelet WT VWF -/- platelet WT KO VWF -/- Proplatelets D.Baruch Platelet formation ISCT 25/04/
13 Role of VWF in terminal platelet production VWF The role of VWF in terminal platelet production is: 1. To allow the conversion of preplatelets to proplatelets 2. To allow terminal fragmentation of proplatelets into platelets Proplatelets: reservoir of platelets ready to replace platelets needed to repair vessel injury Compatible model with small number of megakaryocytes leaving the bone marrow and the large daily circulating platelet production D.Baruch Platelet formation ISCT 25/04/
14 Megakaryocytic differentiation in 2D culture and 3D scaffolds D2 D12 D16 2D liquid culture Immature cells Mature MK D2 D6 D9 3D culture D12 D23 D30 Prolongation of cell survival and MK differentiation up to D30 in 3D scaffolds vs D16 in liquid culture D.Baruch Platelet formation ISCT 25/04/
15 Platelet production in 3D conditions 3D No activation Thrombin activation Liquid culture Increased platelet production in 3D conditions Platelets produced in flow conditions are functional D.Baruch Platelet formation ISCT 25/04/
16 Platelet production from induced pluripotent stem cells Takayama & al. J. Exp. Medicine 2010 The Nobel Prize in Physiology or Medicine 2012 D.Baruch Platelet formation ISCT 25/04/
17 Optimization of platelet production from induced pluripotent stem cells Eto & al. Cell Stem Cell 2014 D.Baruch Platelet formation ISCT 25/04/
18 Perspectives Innovative source of platelets for tomorrow s platelet transfusion? PLASMIS Large-scale platelet production from stem cells in microfluidic devices D.Baruch Platelet formation ISCT 25/04/
19 Acknowledgements INSERM UMR_S1140 and Paris Sorbonne University Claire Dunois-Lardé Sonia Poirault-Chassac Audrey Pietrzyk Alexandre Kauskot Kim-Anh Nguyen PLASTHER CONSORTIUM INSERM U1016 Isabelle Dusanter INSERM U770 Cécile Denis INSERM UMR_S1148 and Paris Sorbonne University Catherine Le Visage Didier Letourneur Mohamed Derkaoui PLASMIS CONSORTIUM UMR CNRS7083 INSERM U1009 Mathilde Reyssat Najet Debili INSERM U1016 INSERM U770 Isabelle Dusanter Cécile Denis Lab Hematology CHU Bichat Paris Nadine Ajzenberg Lab Hematology CHU HEGP Paris Pascale Gaussem Lab Thrombosis Research KU Leuven Hans Deckmyn D.Baruch Platelet formation ISCT 25/04/
Physiology Unit 3 HEMATOLOGY
Physiology Unit 3 HEMATOLOGY In Physiology Today Hematocrit Percentage of blood volume that is erythrocytes 45% in men 42% in women Average blood volume in is 5 L Plasma Large amounts of organic/inorganic
POST-ISTH: Novità dal meeting di Toronto 2015 Piastrine: fisiopatologia. Erica De Candia Università Cattolica -Roma
POST-ISTH: Novità dal meeting di Toronto 2015 Piastrine: fisiopatologia Erica De Candia Università Cattolica -Roma Novità ISTH 2015: Piastrine: Fisiopatologia i) Alternative platelet function throughout
Membrane microvesicles: a circulating source for fibrinolysis, new antithrombotic
Membrane microvesicles: a circulating source for fibrinolysis, new antithrombotic messengers 1 Laurent Plawinski, 2,3 Eduardo Anglés-Cano 1 CNRS UMR 5248 Imagerie Moléculaire et Nanobiotechnologie, Université
Interaction of blood cells with the vessel wall in thrombosis
University of Iowa Iowa Research Online Theses and Dissertations Spring 2014 Interaction of blood cells with the vessel wall in thrombosis Jessica Kay Hansen University of Iowa Copyright 2014 Jessica Kay
LABORATORY APPROACH TO BLEEDING DISORDERS DR NISHANTH PG 1 ST YEAR DEPARTMENT OF PATHOLOGY
LABORATORY APPROACH TO BLEEDING DISORDERS DR NISHANTH PG 1 ST YEAR DEPARTMENT OF PATHOLOGY 1 WHEN IS THE LAB REQUIRED TO INVESTIGATE FOR A POSSIBLE BLEEDING DISORDER? Clinically suspected bleeding tendency
De l'utilisation de la microscopie intravitale pour étudier la thrombose in vivo et tester de nouveaux médicaments antithrombotiques- 1er partie
De l'utilisation de la microscopie intravitale pour étudier la thrombose in vivo et tester de nouveaux médicaments antithrombotiques- 1er partie Christophe Dubois INSERM UMR-S-1076, Faculté de Pharmacie,
Hemostasis and Thrombosis
Hemostasis Hemostasis and Thrombosis Normal hemostasis is a consequence of tightly regulated processes that maintain blood in a fluid state in normal vessels, yet also permit the rapid formation of a hemostatic
Hematology Emergencies: Problems with Platelets
Hematology Emergencies: Problems with Platelets Christian Cable, MD, FACP Associate Professor of Medicine Division of Hematology & Oncology Texas A&M HSC College of Medicine Scott & White Healthcare Fundamentals
Challenges in manufacture of human platelets for transfusion
Challenges in manufacture of human platelets for transfusion David Haylock Materials Science & Engineering 15 th October 2012 CMSE What are platelets? Also called thrombocytes Enucleated cell (or cell
Chapter 19b Blood, cont d
Chapter 19b Blood, cont d White Blood Cells WBCs account for less than 1% of blood volume. There are two major histological categories of WBCs the granulocytes and the agranulocytes. GRANULOCYTES are Basophils,
Department of Research Evaluation. Biology and pharmacology of blood platelets: haemostasis, thrombosis, transfusion
Department of Research Evaluation report on research unit: Biology and pharmacology of blood platelets: haemostasis, thrombosis, transfusion Under the supervision of the following institutions and research
von Willebrand factor: a hemostatic protein
von Willebrand factor: a hemostatic protein!"#$%&'()*+',,-.'/01*+23'45'6*78+95' Cécile Denis INSERM U770 Le Kremlin-Bicêtre, France University of Ferrara October 19th, 2012! Presentation schedule! Basic
Supplementary Figure 1 Pfn1, but not other Pfn isoforms are expressed in
Supplementary Figure 1 Pfn1, but not other Pfn isoforms are expressed in platelets. (a) RT-PCR of Pfn isoforms in control mouse platelets, Pfn1 -/- platelets and control heart. Expected band size for Pfn1
At the conclusion of this lesson you should be able to:
Learning Objectives At the conclusion of this lesson you should be able to: Understand the key terms and definitions regarding stem cells Differentiate between the adult and embryonic stem cells Differentiate
(B) Comparable expression of major integrin subunits and glycoproteins on the surface of resting WT and Lnk -/- platelets.
Supplemental Figure S1. Characteristics of Lnk -/- platelets. (A) Electron micrographs of resting platelets showing the normal intracellular structure of Lnk -/ platelets. Samples were fixed with 4% PFA
Hemostasis. by Mohie-Aldien Elsayed
Hemostasis by Mohie-Aldien Elsayed Hemostasis Hemostasis is defined as the process that maintains the flowing blood in a fluid state and confined to the circulatory system Coagulation prevents excess blood
engineering microvasculature-on-chip models as research-enabling systems for hemostasis and thrombosis
engineering microvasculature-on-chip models as research-enabling systems for hemostasis and thrombosis Wilbur A. Lam, MD, PhD Associate Professor Wallace H. Coulter Department of Biomedical Engineering
The Cardiovascular System: Blood
The Cardiovascular System: Blood The Functions of Blood General Overview Provides a system for rapid transport within the body Nutrients Hormones Waste products Respiratory gases Cells & Blood Components
The Coagulation Workup In The Office Setting
CAA Conference alm Springs 2012 The Coagulation Workup In The Office Setting CAA Conference alm Springs, October 2012 Robert Miller, A-C Henoch-Schoenlein urpura 1 CAA Conference alm Springs 2012 2 CAA
Sign up to receive ATOTW weekly -
BLOOD PHYSIOLOGY PART 2 ANAESTHESIA TUTORIAL OF THE WEEK 231 11 TH JULY 2011 Dr Karen Hayes Royal Devon & Exeter Correspondence to: kmhayes@hotmail.co.uk QUESTIONS Before continuing, try to answer the
Ch 13: Blood. What does blood do? Transport: Regulation: Protection:
Ch 13: Blood What does blood do? Transport: Regulation: Protection: SLOs Describe the composition of plasma and list the major functions of plasma proteins. Map hematopoiesis, starting from a pluripotent
Local vasoconstriction. is due to local spasm of the smooth muscle (symp. reflex) can be maintained by platelet vasoconstrictors
Hemostasis Hemostasis ( hemo =blood; sta= remain ) is the stoppage of bleeding, which is vitally important when blood vessels are damaged. Following an injury to blood vessels several actions may help
International Society Laboratory Hematology Milan, May Current and Emerging Approaches for Assessing von Willebrand disease (VWD) in 2016
International Society Laboratory Hematology Milan, 12-14 May 2016 Current and Emerging Approaches for Assessing von Willebrand disease (VWD) in 2016 Augusto B. FEDERICI Hematology and Transfusion Medicine
NEXT GENERATION ECM-BASED ALLOGRAFT TECHNOLOGY:
NEXT GENERATION ECM-BASED ALLOGRAFT TECHNOLOGY: Potent biological scaffolds strategically control stem cell fate and function, allowing our allografts to harness the regenerative potential of patient s
Farnesoid X Receptor and its ligands inhibit the function of platelets
Farnesoid X Receptor and its ligands inhibit the function of platelets Article Accepted Version Figures and Legends Moraes, L. A., Unsworth, A. J., Vaiyapuri, S., Ali, M. S., Sasikumar, P., Sage, T., Flora,
Platelet Adhesion and Aggregation Assays
PPLICTION NOTE Platelet dhesion and ggregation ssays Microfluidic assays enable multi-parametric investigation of platelet behavior under flow. Relevant Research reas: Vascular Biology, Thrombosis and
OVERVIEW OF LABORATORY DIAGNOSTIC TESTING FOR PLATELET DISORDERS
OVERVIEW OF LABORATORY DIAGNOSTIC TESTING FOR PLATELET DISORDERS Catherine P. M. Hayward, MD PhD, FRCP(C) Head, Coagulation, Hamilton Regional Laboratory Medicine Program Professor, Pathology and Molecular
Alternativmethodezumknock-out Modell in der Arterioskleroseforschung: Sascha Meyer dos Santos
Alternativmethodezumknock-out Modell in der Arterioskleroseforschung: humane Arterien in flow-based adhesion assays Sascha Meyer dos Santos 07.11.13 Replacement, Reduction, Refinement aktueller denn je?
Common Inherited Bleeding Disorders
CAA 2015 Annual Conference Common Inherited Bleeding Disorders Bob Miller, A October 8, 2015 VWF has two jobs Loosely bound to protect FVIII and tether to site of injury All other coagulation factors also
Activation and specificity of Thrombin. Giulia Pavani
Activation and specificity of Thrombin Giulia Pavani Summary Regulation of a Serine Protease: Thrombin Zymogen Enzyme Substrate Specificity Staphylocoagulase Bacteria know how a protease works (much more
Overview of Anatomy and Physioloy II Second Year Students
University of Baghdad College of Nursing Department of Basic Medical Sciences Overview of Anatomy and Physioloy II Second Year Students Asaad Ismail Ahmad, Ph.D. Asaad Ismail Ahmad, Ph.D. Electrolyte and
Profibrinolytic diabody targeting PAI-1 and TAFI for treatment of acute thrombotic disorders
ProFiDIΛ Profibrinolytic diabody targeting PAI-1 and TAFI for treatment of acute thrombotic disorders THROMBOLYSIS WITH IMPROVED SAFETY A valorisation project of Unmet Medical Need = Safe Thrombolysis
UvA-DARE (Digital Academic Repository) Factor XI as target for antithrombotic therapy van Montfoort, M.L. Link to publication
UvA-DARE (Digital Academic Repository) Factor XI as target for antithrombotic therapy van Montfoort, M.L. Link to publication Citation for published version (APA): van Montfoort, M. L. (2014). Factor XI
Disclaimer: this is a very big topic and coverage will be only superficial.
Vascular Biology 4 Blood and its components: 1 Disclaimer: this is a very big topic and coverage will be only superficial. 1. Erythrocytes: eat your spinach (or steak). ~8 um diameter, 2 um thickness no
THE SCIENCE OF PLATELET RICH PLASMA
THE SCIENCE OF PLATELET RICH PLASMA Cheri A. Ong, MD FACS ONG INSTITUTE FOR PLASTIC SURGERY & HEALTH WHAT IS PRP BLOOD PLASMA 55% of the body s today body volume 45% blood cells:- Red blood cells, White
Regulation of Hematopoietic Stem Cells and their Bone Marrow Niches by the Coagulation System*.
Regulation of Hematopoietic Stem Cells and their Bone Marrow Niches by the Coagulation System*. * Via PAR-1 & CXCR4 upregulation, SDF-1 secretion and EPCR shedding. By Tsvee Lapidot, Ph.D. Weizmann Institute
Platelet Refractoriness: The Basics. Martin H. Bluth, MD, PhD
Platelet Refractoriness: The Basics Martin H. Bluth, MD, PhD Complete Toxicology Laboratories, LLC Objectives Define platelet refractoriness and associated conditions that may cause platelet refractoriness.
BLOOD COAGULATION. 1. The initial phase of the process is vascular constriction. This limits the flow of blood to the area of injury.
BLOOD COAGULATION The ability of the body to control the flow of blood following vascular injury is paramount to continued survival. The process of blood clotting and then the subsequent dissolution of
Platelet deposition (%) Fibronectin
Platelet deposition (%) Platelet deposition (%) 60 p = 0.347 60 p = 1.000 40 p = 0.881 40 20 20 p = 0.114 0 Up Down Up Down 0 Up Down Up Down Fibrinogen Collagen I Fibronectin Collagen I Supplementary
Chapter 8 Healthcare Biotechnology
Chapter 8 Healthcare Biotechnology Outline: 8.1 Introduction 8.2 Biopharming 8.3 Models of Human Disease 8.4 Detecting and Diagnosing Human Disease 8.5 Monoclonal Antibodies 8.6 Gene Therapy 8.7 Tissue
بسم اهلل الرمحن الرحيم
بسم اهلل الرمحن الرحيم Definition Multiple myeloma is a neoplastic plasma cell dyscrasia (PCD) that generally produced a monoclonal immunoglobulin protein, characterized by a clinical pentad: (a) anemia;
Coagulopathy Case-2. Andy Nguyen, M.D. 2009
Coagulopathy Case-2 Andy Nguyen, M.D. 2009 CLINICAL HISTORY A 42 year-old man brought to the emergency room with severe burn. Patient was rescued by firemen in a serious fire. He had been found unconscious
SUPPLEMENTAL MATERIAL. Supplemental Methods:
SUPPLEMENTAL MATERIAL Supplemental Methods: Immunoprecipitation- As we described but with some modifications [22]. As part of another ongoing project, lysate from human umbilical vein endothelial cells
Adult Stem Cells for Chronic Pain. Dr. John Hughes, DO January 24 th, 2018
Adult Stem Cells for Chronic Pain Dr. John Hughes, DO January 24 th, 2018 Dr. John Hughes, DO Doctor of Osteopathy From Georgia Arizona College of Osteopathic Medicine - 2007 Aspen Integrative Medicine
General Principles of. Hemostasis. Kristine Krafts, M.D.
General Principles of Hemostasis Kristine Krafts, M.D. Hemostasis is a balancing act! pro-ting plugs up holes in blood vessels anti-ting keeps ting under control Pro-Clotting Pro-Clotting vessels platelets
SmartPReP 2: The Gold Standard
The Optimal Platelet Rich Plasma Composition SmartPReP 2: The Gold Standard Harvest Technologies is the leader in developing point-of-care cellular platforms to isolate and concentrate autologous growth
Discover TruPRP. PRP the way you want it.
Discover TruPRP PRP the way you want it. Discover TruPRP Discover the quality of Magellan TruPRP. The Magellan technology provides an automated dual spin processing system that can deliver (PRP) Platelet
Supplement Figure 1. Characterization of the moab. (A) A series of moabs that are anti-hαiib-specific were tested for their ability to bind to
Supplement Figure 1. Characterization of the 312.8 moab. (A) A series of moabs that are anti-hαiib-specific were tested for their ability to bind to platelets. The black line represents the 312.8 moab
Syringe Pumps. Please contact us for help in selecting the best pump for your application and a free quote.
Syringe Pumps Microfluidic Pumps! We are proud to offer select Harvard Apparatus syringe pumps for use with SynVivo biochips. These high quality pumps have been extensively tested and offer a feature rich
Session 1 Topics. Vascular Phase of Hemostasis. Coagulation Pathway. Action of Unfractionated Heparin. Laboratory Monitoring of Anticoagulant Therapy
~~Marshfield Labs Presents~~ Laboratory Monitoring of Anticoagulant Therapy Session 1 of 4 Session 1 Topics Review of coagulation and the vascular phase of hemostasis Unfractionated heparin Low molecular
These handouts are only meant as a guide while you follow the presentation on the screen. Sometimes the speaker will change some of the slides.
These handouts are only meant as a guide while you follow the presentation on the screen. Sometimes the speaker will change some of the slides. If you would like the 1 slide per page handouts, please ask
PROVINCIAL BLOOD COORDINATING PROGRAM DEFINITIONS NLBCP-063
Government of Newfoundland and Labrador Department of Health and Community Services Provincial Blood Coordinating Program PROVINCIAL BLOOD COORDINATING PROGRAM DEFINITIONS Office of Administrative Responsibility
Coagulation Mechanisms Dr. Nervana Bayoumy
Coagulation Mechanisms Dr. Nervana Bayoumy Associate Professor Department of Physiology Objectives At the end of this lecture you should be able to: 1. Recognize the different clotting factors 2. Understand
Common Inherited Coagulation Disorders. Inherited Coagulation Disorders. All coagulation factors and platelets in circulation (unactivated)
Common Inherited Coagulation Disorders Bob Miller, A 2017 Inherited Coagulation Disorders Brief review of coagulation Disorders related to platelet dysfunction (not the acquired thrombocytopenias) Disorders
Plasma fibronectin supports hemostasis and regulates thrombosis
The Journal of Clinical Investigation Plasma fibronectin supports hemostasis and regulates thrombosis Yiming Wang, 1,2,3 Adili Reheman, 2 Christopher M. Spring, 2 Jalil Kalantari, 2,4 Alexandra H. Marshall,
Case 1. Case 1. Case 2. Case 2. Review of Bleeding Disorders Case 1
Review of Bleeding Disorders Case 1 Robert I. Handin, MD Professor of Medicine, Harvard Medical School Hematology Division Brigham & Women s Hospital 20 year college student is home on spring break and
Platelets in mammals are anucleated cells that originate. Reviews. Adhesion Mechanisms in Platelet Function
Reviews This Review is part of a thematic series on Mechanisms, Models, and In Vivo Imaging of Thrombus Formation, which includes the following articles: Activation of Platelet Function Through G Protein
Regulation of advanced blood cell therapies
Regulation of advanced blood cell therapies www.pei.de Clinical trials using cell-based products Substantially manipulated cells and cells for non-homologous use Quality, safety and non-clinical aspects
Stem Cell Research From Bench to Bedside
Stem Cell Research From Bench to Bedside Stem Cell Dialogues Jan A. Nolta, Ph.D. Professor, Internal Medicine Director, UC Davis Institute for Regenerative Cures October 26, 2010 Stem Cells at UC Davis
Person responsible (Chair): Martine Jandrot-Perrus, Hans Deckmyn
NAME OF PROJECT: Measurement of platelet activation markers: usefulness, current practices, future. Subcommittee: Platelet physiology Person responsible (Chair): Martine Jandrot-Perrus, Hans Deckmyn Design:
Introduction Hemostasis: Tourniquet Test & Bleeding Time. Hematology-Immunology System Faculty of Medicine Universitas Padjadjaran LOGO
Introduction Hemostasis: Tourniquet Test & Bleeding Time Hematology-Immunology System Faculty of Medicine Universitas Padjadjaran LOGO Hemostasis A series of reactions that function to stop bleeding, maintaining
Understanding Megakaryopoiesis And Thrombopoiesis Using Human Stem Cells Models
University of Pennsylvania ScholarlyCommons Publicly Accessible Penn Dissertations 217 Understanding Megakaryopoiesis And Thrombopoiesis Using Human Stem Cells Models Xiu Li Sim University of Pennsylvania,
HIGH-THROUGHPUT HUMAN TISSUE AND DISEASE MODELS. Organ on a chip. Now.
HIGH-THROUGHPUT HUMAN TISSUE AND DISEASE MODELS Organ on a chip. Now. High-throughput human tissue and disease models We all want better medicines, that s obvious. To make this possible, you need better
KILLING THROMBUS WITH
KILLING THROMBUS WITH D. Dash Department of Biochemistry Institute of Medical Sciences Banaras Hindu University Thrombus has two components: (1) Protein Component composed of Insoluble Fibrin Clot (2)
Dr. Susan Louw Haematopathologist NHLS / WITS / SASTH
Dr. Susan Louw Haematopathologist NHLS / WITS / SASTH Since 1948 Initially a research tool Utility in management of bleeding and thrombosis Guide clotting factor replacement, platelet / blood transfusions
Personal history of excessive mucocutaneous bleeding. Family history of excessive bleeding. Laboratory tests of hemostasis consistent with VWD
Diagnostic Approach Personal history of excessive mucocutaneous bleeding Family history of excessive bleeding Laboratory tests of hemostasis consistent with VWD Nose Bleeds Skin Bruising Gum Bleeding Menorrhagia
Clinical Policy: Humate-P (Antihemophiliac Factor/von Willebrand Factor Complex Human) Reference Number: CP.MP.404
Clinical Policy: (Antihemophiliac Factor/von Willebrand Factor Complex Human) Reference Number: CP.MP.404 Effective Date: January 2008 Last Review Date: 12/16 See Important Reminder at the end of this
Generation of HLA universal platelets for regenerative applications
Generation of HLA universal platelets for regenerative applications Constança Figueiredo Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany HLA loci are the most polymorphic
SUPPLEMENTAL FIGURES/TABLES:
SUPPLEMENTAL FIGURES/TABLES: Journal: Nature Biotechnology Article Title: Correction of the coagulation defect in hemophilia using a factor Xa variant with novel engineered protease function. Authors:
Factor Concentrates. More is better? Alexander Duncan MD Emory Medical Labs
Factor Concentrates. More is better? Alexander Duncan MD Emory Medical Labs Call from OR! Scenario -1 Patients is bleeding out! Don t ask why, what rate what s been done! We need blood now! What kind of
What Can We Learn from Animal Models of Coagulopathy and Bleeding (about plasma transfusion)?
Test What Can We Learn from Animal Models of Coagulopathy and Bleeding (about plasma transfusion)? William Sheffield Associate Director, Research CBS Centre for Innovation, and Professor, Pathology and
Scientific Workshop. Melbourne Convention and Exhibition Centre Level 2, Meeting Rooms 218 and 219 Melbourne
Scientific Workshop Melbourne Convention and Exhibition Centre Level 2, Meeting Rooms 218 and 219 Melbourne 12 th November 2016 Australasian Society of Thrombosis and Haemostasis The Australasian Society
A revised model of platelet aggregation
A revised model of platelet aggregation Suhasini Kulkarni, 1 Sacha M. Dopheide, 1 Cindy L. Yap, 1 Catherine Ravanat, 2 Monique Freund, 2 Pierre Mangin, 2 Kathryn A. Heel, 1 Alison Street, 3 Ian S. Harper,
Inhibition of the prostaglandin-degrading enzyme 15-PGDH potentiates tissue regeneration. JournalClub Emilie Hrdliczka
Inhibition of the prostaglandin-degrading enzyme 15-PGDH potentiates tissue regeneration JournalClub 14.12.2015 Emilie Hrdliczka Facts Author: Yongyou Zhang Department of Medicine, Case Western Reserve
Supplementary Figure 1 Caspase-8 deficient platelets respond normally to agonists and ABT-737 (A) Surface expression of GPIX, GPIb, GPVI and CD41
Supplementary Figure 1 Caspase-8 deficient platelets respond normally to agonists and ABT-737 (A) Surface expression of GPIX, GPIb, GPVI and CD41 were assessed on purified platelets from Casp8 fl/fl and
BUILT TO CONCENTRATE. Magellan is an autologous concentration system that delivers concentrated platelets and cells at the point of care.
BUILT TO CONCENTRATE Magellan is an autologous concentration system that delivers concentrated platelets and cells at the point of care. DELIVER PERSONALIZED MEDICINE Every patient has a unique biology
Cardiovascular (connective tissue)
Cardiovascular (connective tissue) Blood Connective tissue Blood Proteins 7% Plasma 55% Other solutes 2% Water 91% Erythrocytes ~5.2 million per cubic mm Formed elements 45% Leukocytes ~7 thousand per
-- Laki J. Rousou, MS, MD et. al. -- Boston, Massachusetts. -- Ann Thorac Surg 2009;87:62 70.
-- Laki J. Rousou, MS, MD et. al -- Boston, Massachusetts. -- Ann Thorac Surg 2009;87:62 70. Presented by: Int. 黃柏翔 Date: 2009 /02 / 25 Patient outcome after CABG surgery greatly influenced by patency
SMART HEALTH PROJECT
SMART HEALTH PROJECT SEDICIDODICI srl is a biomedical company constituted by a consolidated management and scientific expert team, focused on the development of a NOVEL EX VIVO DIAGNOSTIC MICROFLUIDIC
Present and future of regenerative medicine. Liver Transplantation
Present and future of regenerative medicine Liver Transplantation Mireia Caralt, MD PhD Servei Cirurgia HBP i Trasplantaments March 19, 2015 Introduction Strategies to increase the number of organs EXPAND
The Power in You. TM
RegenKit THT Autologous Platelet Rich Plasma (A-PRP) The Power in You. TM Biologics Concentrated on Healing RegenKit THT Autologous Platelet Rich Plasma (A-PRP) The RegenKit THT is designed to be used
LABORATORY APPROCH TO THE BLEEDING PATIENT
Anne Winkler, MD Emory University School of Medicine, Atlanta Georgia Assistant Professor, Pathology & Laboratory Medicine Medical Director, Transfusion Service, Grady Health System Assistant Director,
Developing Targeted Stem Cell Therapeutics for Cancer. Shawn Hingtgen, Ph.D. Assistant Professor UNC Eshelman School of Pharmacy May 22 nd, 2013
Developing Targeted Stem Cell Therapeutics for Cancer Shawn Hingtgen, Ph.D. Assistant Professor UNC Eshelman School of Pharmacy May 22 nd, 2013 The Challenge of Drug Delivery for Brain Cancer Stem Cells
VIII. Validation Evaluation Comparative Chart Application Fields of PRP Advantages of MiroPRP for skin treatments MicroPRP and Mico-Mesotherapy
The New Technology for Safe Platelet Activation INDEX I. Blood Composition II. What is PRP Therapy? III. Growth Factors Present in PRP IV. Why is PRP concentration significant? V. How are Platelets Activated?
Platelet type bleeding: (vwd) - Petechiae, purpura, ecchymoses, bruising - Menorrhagia, hematuria, occult GI bleed - Gingival bleeding, epistaxis
1 Nadanotes.com 2 Nadanotes.com 3 Nadanotes.com Platelet type bleeding: (vwd) - Petechiae, purpura, ecchymoses, bruising - Menorrhagia, hematuria, occult GI bleed - Gingival bleeding, epistaxis Clotting
Alberta Health Services - Edmonton Zone Massive Hemorrhage Protocol
This document applies to all Covenant and AHS sites. Client Resource 23: Alberta Health Services - Edmonton Zone Regional Laboratory Services This document is applicable at site(s): GNH MIS RAH SGH UAH
Adv Pathophysiology Unit 4 Page 1 of 10. Learning Objectives for this file:
Adv Pathophysiology Unit 4 Page 1 of 10 Learning Objectives for this file: Topics include structure & function of: 1. Coagulation process platelets, clotting factors 2. Coagulation cascade formation of
ANAT 3231 Cell Biology Lecture 21 Stem Cells
ANAT 3231 Cell Biology Lecture 21 Stem Cells Outline What are Stem Cells? Totipotency - Pluripotency - Multipotency What are different sources of Stem Cells? Embryonic vs Adult Pros and Cons for each type
Bivalirudin (Angiomax ) vs. Heparin during Peripheral Vascular Interventions: Which is Better and How to Decide?
Bivalirudin (Angiomax ) vs. Heparin during Peripheral Vascular Interventions: Which is Better and How to Decide? Vinod Nair MD FACC FSCAI Cardiovascular Ins6tute of the South, Houma Financial Disclosure:
SUPPLEMENTAL MATERIAL. von Willebrand Factor unfolding and elongation on collagen during acute whole blood exposure to pathological flow
SUPPLEMENTAL MATERIAL von Willebrand Factor unfolding and elongation on collagen during acute whole blood exposure to pathological flow T. V. Colace and S. L. Diamond Institute for Medicine and Engineering
Isolation of mouse monocytes: Mouse monocytes were isolated using a modified
Supplemental Material Extended Methods Isolation of mouse monocytes: Mouse monocytes were isolated using a modified method described (1). Citrated mouse whole blood was mixed with equal volume of PBS,
Microparticles in platelet concentrates are a key indicator of the level of platelet activation. This is a topic that is critically important to
Microparticles in platelet concentrates are a key indicator of the level of platelet activation. This is a topic that is critically important to understand in more detail. Based on the literature between
ANAT 2341 Embryology Lecture 18 Stem Cells
ANAT 2341 Embryology Lecture 18 Stem Cells 29 September 2010 Dr Antonio Lee Neuromuscular & Regenera
10/17/ /17/2017
Apheresis Objectives: Explain the concept of cell centrifugation Explain the concept of extracorporeal volume Name 2 methods of venous access for apheresis 1 What is Apheresis? Elements: Whole blood removal
UNCLASSIFIED AD DEFENSE DOCUMENTATION CENTER FOR SCIENTIFIC AND TECHNICAL INFORMATION CAMERON STATION, ALEXANDRIA. VIRGINIA UNCLASSIFIED
UNCLASSIFIED AD 408 690 DEFENSE DOCUMENTATION CENTER FOR SCIENTIFIC AND TECHNICAL INFORMATION CAMERON STATION, ALEXANDRIA. VIRGINIA K UNCLASSIFIED NOTICE: When government or other drawings, specifications
Cellular repair of damaged organs. Repopulating scaffoldings in kidney and liver
Cellular repair of damaged organs Repopulating scaffoldings in kidney and liver Mireia Caralt, MD PhD Servei Cirurgia HBP i Trasplantaments March 29, 2017 Introduction Strategies to increase the number
SUPPLEMENTARY FIGURE 1
SUPPLEMENTRY FIGURE 1 C D E F G * Supplementary Fig. 1: Platelets from Pik3c2b mice exhibit normal function. () Expression of platelet-specific glycoproteins on the surface of platelets in whole blood
STANDARD BLOOD PRODUCTS AND SERVICES
STANDARD BLOOD PRODUCTS AND SERVICES Policy NHP reimburses contracted providers for the medically necessary administration (transfusion) of blood and standard blood products. Prerequisites Authorization,
Dr Alain Gondinet of ReGen Lab reviews cell therapies using platelet rich plasma autologous cellular regeneration
BioDiscovery Technology Tissue Regeneration Dr Alain Gondinet of ReGen Lab reviews cell therapies using platelet rich plasma autologous cellular regeneration This article describes the potential for doctors