Prof. M. Scott Shell TA: Sunyia Hussein
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1 ChE170 Molecular and Cell Biology for Engineers TuTh 11-12:15 Prof. M. Scott Shell TA: Sunyia Hussein 1
2 2
3 the number of civilizations in our galaxy with which communication might be possible 3
4 rateofstarformation/year(inourgalaxy) ~7 4
5 fractionofstarswithplanets ~50% 5
6 average number of life-supporting planets per star with planets ~ 2 6
7 fractionthatactuallyproceedtodeveloplife ~33% 7
8 fraction that proceed to develop intelligent life ~ 1% 8
9 fraction of civilizations that develop technology to make themdetectiblefromspace ~1% 9
10 length of time that these civilizations release detectable signals ~10000years 10
11 11
12 12
13 13
14 14
15 Miller Urey Experiment (1952) 15
16 Old view: Biology entailshighly specialized chemistry and physics to accomplish complex tasks. New view: Biology uses very general chemical and physical principles,and illustrates how they may be used to accomplish complex tasks. 16
17 Old view: Chemistry and physics are foundational sciences for Chemical Engineering. New view: Biology is a foundation for Chemical Engineering on equal footing with chemistry and physics. 17
18 Employment of chemical engineering class of 2009 source: CEP Magazine, 9/09 18
19 Why should engineers know molecular and cell biology? thermodynamics & physical chemistry transport phenomena kinetics systems level analysis models of cellular processes knowledge of cellular components, interactions, and mechanisms 19
20 Why should engineers know molecular and cell biology? Biochemical Engineering & Biotechnology Alternative energy Biofuels production: ethanol, cellulosic conversions, sugar to alkanes Metabolic engineering cellular engineering, genetic engineering Systems biology drug delivery (e.g. insulin), fuels Synthetic biology designer biological systems Drug development and production Chemical Engineers are uniquely equipped to address these problems, using kinetics, thermodynamics and transport phenomena. 20
21 Chemical Engineering (UCB) awarded a $42 million grant from the Bill & Melinda Gates Foundation, to produce artemisinicacid, a prescursor to the anti-malarial drug artemisinin. 21
22 Rethinking Mother Nature's Choices Jay Keasling believes ethanol is a poor biofuel. So he's going to get microbes to make something better. Robert F. Service 22
23 Frances Arnold (Caltech) Custom-Made Microbes, at Your Service ChEProfessor Frances Arnoldis using synthetic biology and protein engineering to design better enzymes for making biofuels from cellulose. 23
24 Biochemical Engineering for Bioproducts Production Drugs (e.g., antibiotics) Foods Food additives Beverages Special Chemicals F, S 0 Harvest V, X, S, P 24
25 Fed Batch Mass Balances Cell dxv dt = µ XV Substrate dsv 1 1 = FS f µ XV qp XV dt Y Y X / S P / S Product dpv dt = q XV p Total Mass dv dt = F (assumes constant density) Monod equation for specific growth rate µ = µ maxs K + S S 25
26 Why should engineers know molecular and cell biology? Biomedical Engineering Tissue engineering Device engineering drug pumps, artificial organs Drug delivery Advanced Study Graduate School Law Medicine Chemical Engineers are uniquely equipped to address these problems, using kinetics, thermodynamics and transport. 26
27 Glucose concentration Control algorithms enable automated delivery of insulin to Diabetics 27
28 Systems Biology: Mathematical Descriptions of Cellular and Physiological Phenomena UCSB and Pfizer Enter into Research Consortium to Expand Understanding of Diabetes and Obesity Pathobiology UC Santa Barbara s Institute for Collaborative Biotechnologies has joined into a research consortium with Pfizer, three other major research universities Caltech, Massachusetts Institute of Technology, and the University of Massachusetts and Entelos, a physiological modeling company, to seek out new targets for drugs to treat diabetes. Pfizer is funding the three-year, $14 million Insulin Resistance Pathway (IRP) Project to look at insulin signaling in adipose (fat) cells to increase understanding of diabetes and obesity, inextricably linked conditions which affect 7 percent of the US population. 28
29 experiments neurons in the brain in tissue cultures alone [Herzog et al, JBR, 2004] [To et al, Biophys J, 2007] 29
30 neuron neuron 30
31 Delivery of therapeutics from blood vessels to tumors is influenced by transport convection, diffusion kinetics binding, internalization, degradation Tumor 31
32 A [AB] D P A k B U A What is [AB](t) =?? 32
33 33
34 Why should engineers know molecular and cell biology? Biology suggests new ways of nanoscale engineering Nanomaterials Nanodevices Nanomachines Chemical Engineers are uniquely equipped to address these problems, using kinetics, thermodynamics and transport. 34
35 DNA staples 35 Rothemund, Science, 2006.
36 unstructured (solubilized) peptides (solution) sequence, temperature, concentration, ph, salt additives fibrils and aggregates networks and gels micelles, nanotubes, 36
37 37 Zhang, Nature, 2003.
38 109C Topics Essential to an understanding of molecular and cell biology Carbohydrates & lipids Amino acids, peptides, and proteins Mechanisms of catalysis Nucleosides, nucleotides, and nucleic acids 38
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