BD Resurge Media Supplements Chemically Defined. Protein Free. Animal Free.

Transcription

1 D Resurge Media Supplements Chemically Defined. Protein Free. nimal Free. Overview Cell-based production of active pharmaceutical ingredients generally requires supplementation of cell culture media to promote cell growth and boost protein production. Every cell line can offer unique challenges for developing processes that yield desired performance criteria. One bioprocess challenge is the selection and optimization of a cell culture supplement or feed that will result in desired titer, growth characteristics, and protein quality. Therefore, the availability of a family of chemically defined (CD) supplements offering a diverse performance profile can significantly improve process development timelines. D iosciences dvanced ioprocessing has designed and formulated a diverse set of fully chemically defined (CD), animal free (F), and protein free cell culture supplements, D Resurge TM CD-CD supplements, without glucose and L-glutamine. This set of supplements can be used in batch and fed-batch processes to enhance recombinant protein production in mammalian cells while maintaining product quality. These supplements have been developed with multiple CHO host cell lines and in various culture systems including shaker flasks and bench-scale bioreactors. single supplement may not be ideal for enhancing performance across multiple cell line conditions; therefore a wider array of CD supplement formulations may be more effective for process development. pplication in atch and Fed atch Modes-Shaker Flasks D Resurge CD-CD supplements can be used in batch and fed batch modes in cell culture processes. D Resurge CD Supplements in ioreactor Performance of D Resurge CD supplements is scalable to bioreactors. The five D Resurge CD supplements produced a --fold increase in protein production over media control in bench top bioreactors in batch or fed batch mode. Equivalent results were obtained in shaker flask studies, indicating successful scalability.

2 D Resurge Media Supplement Performance, atch and Fed atch Mode Representative shaker flask and bioreactor studies are shown below for D Resurge CD-CD. In each figure panel shows batch and fed batch data using shaker flasks, while panel shows bioreactor data. D Resurge CD 8 9 VCD (e cells/ml) 7 VCD (e cells/ml) D Resurge CD-atch Production D Resurge CD-Fed atch Production D Resurge CD-atch VCD D Resurge CD-Fed atch VCD Production D Resurge CD-Production -VCD D Resurge CD-VCD Performance of D Resurge CD in batch and fed batch mode () Growth and production of CHO Cell Line supplemented with D Resurge CD in batch and fed batch mode in ml shaker flasks. Fed batch cultures were supplemented from early- to mid-phase. () Growth and production of CHO Cell Line supplemented with D Resurge CD in L stir tank bioreactor. The study was performed in fed batch mode with the addition of D Resurge CD on and. The control represents an optimized basal bioreactor process. D Resurge CD VCD (e cells/ml) D Resurge CD-atch Production D Resurge CD-atch VCD D Resurge CD-Fed atch Production D Resurge CD-Fed atch VCD VCD (e cells/ml) Production D Resurge CD-Production -VCD D Resurge CD-VCD Performance of D Resurge CD in batch and fed batch mode () Growth and production of CHO Cell Line supplemented with D Resurge CD in batch and fed batch mode in ml shaker flasks. Fed batch cultures were supplemented from early- to mid-phase. () Growth and production of CHO Cell Line supplemented with D Resurge CD in L stir tank bioreactor. The study was performed in fed batch mode with the addition of D Resurge CD on and. The control represents an optimized basal bioreactor process.

3 D Resurge Media Supplement Performance, atch and Fed atch Mode D Resurge CD 7 VCD (e cells/ml) 8 VCD (e cells/ml) 7 9 D Resurge CD-atch Production D Resurge CD-atch VCD D Resurge CD-Fed atch Production D Resurge CD-Fed atch VCD Production D Resurge CD-Production -VCD D Resurge CD-VCD Performance of D Resurge CD in batch and fed batch mode () Growth and production of CHO Cell Line supplemented with D Resurge CD in batch and fed batch mode in ml shaker flasks. Fed batch cultures were supplemented from early- to mid-phase. () Growth and production of CHO Cell Line supplemented with D Resurge CD in ml microbioreactor. The study was performed using an optimized batch-mode process for both conditions. D Resurge CD 8 8 VCD (e cells/ml) VCD (e cells/ml) 7 9 D Resurge CD-atch Production D Resurge CD-Fed atch Production D Resurge CD-atch VCD D Resurge CD-Fed atch VCD Production D Resurge CD-Production -VCD D Resurge CD-VCD Performance of D Resurge CD in batch and fed batch mode () Growth and production of CHO Cell Line supplemented with D Resurge CD in batch and fed batch mode in ml shaker flasks. Fed batch cultures were supplemented from early- to mid-phase. () Growth and production of CHO Cell Line supplemented with D Resurge CD in L stir tank bioreactor. The study was performed using an optimized batch-mode process for both conditions.

4 D Resurge Media Supplement Performance, atch and Fed atch Mode D Resurge CD 8 VCD (e cells/ml) VCD (e cells/ml) D Resurge CD-atch Production D Resurge CD-atch VCD D Resurge CD-Fed atch Production D Resurge CD-Fed atch VCD -Production D Resurge CD-Production -VCD D Resurge CD-VCD Performance of D Resurge CD in batch and fed batch mode () Growth and production of CHO Cell Line supplemented with D Resurge CD in batch and fed batch mode in ml shaker flasks. Fed batch cultures were supplemented from early- to mid-phase. () Growth and production of CHO Cell Line supplemented with D Resurge CD in L stir tank bioreactor. The study was performed using an optimized batch-mode process for both conditions.

5 D Resurge Media Supplement Comparability and Performance CHO Line CHO Line 8 8 Ctrl CD CD CD CD CD C D E F G H Ctrl CD CD CD CD CD C D E F G H D Resurge Supplements Commercial Supplements D Resurge Supplements Commercial Supplements 9 CHO Line {Comparison of D Resurge CD-CD and competitor supplement performance in multiple CHO cell lines} 8 7 Comparability with commercially available supplements D Resurge CD-CD and eight commercial cell culture supplements were tested for both their effect on growth (data not shown) and production. Each cell line was cultured in an optimized medium. The study was performed in batch mode. ll supplements were used as per the manufacturers recommended protocol. Protein yields with D Resurge supplements were generally equivalent or superior to results obtained using competitor supplements. 8 Ctrl CD CD CD CD CD C D E F G H D Resurge Supplements Commercial Suppl Medium Medium Medium C CHO Line Medium Medium Medium C CHO Line Performance of D Resurge CD-CD supplements in multiple base media across two CHO cell lines that express mb s at different baseline titers Performance of D Resurge CD-CD Supplements in Multiple Media D Resurge CD-CD supplements were evaluated in multiple chemically defined commercial and in-house base media for impact on growth (data not shown) and production across two different CHO cell lines. The study was performed in batch mode.

6 D Resurge Media Supplement Protein Quality Fig. Representative elution profile of glycans from mb isolated from CHO line supplemented with D Resurge CD. Protein Quality with D Resurge CD Supplements How a CD supplement impacts protein quality of a therapeutic antibody is dependent on multiple factors: the type of expression host, the type of base media, and the cell culture process. The end user would have the best understanding of the critical quality attributes of their product, and ultimately preclinical and clinical studies would determine the therapeutic impact of any glycan changes. The N-glycan profiles of monoclonal antibodies from D Resurge supplemented and control conditions were analyzed. typical elution profile is shown for the D Resurge CD supplemented condition in CHO line (Figure ). The structures and relative composition of the most prevalent glycans from each cell line are depicted in Figures (CHO line ) and (CHO line ). Differences between the supplemented and control conditions were similar to those seen when using other supplements, such as peptones (data not shown).

7 D Resurge Media Supplement Protein Quality Protein Quality with D Resurge CD Supplements Fig. Relative glycan composition of mb isolated from CHO line supplemented with D Resurge CD and CD. Fig. Relative glycan composition* of mb isolated from CHO line supplemented with D Resurge CD, CD, and CD. *Some unidentified species (Glycans through ) in CHO line were most likely due to high mannose and/or sialylated glycans; the levels of these components appeared to vary little with supplementation conditions.

8 Ordering Information D Resurge CD-CD supplements are manufactured at the D iosciences dvanced ioprocessing nimal Free and ntibiotic Free (F ), state of the art facility. ll the components are chemically defined with known molecular formulae and structures and meet D s nimal Origin Free Specification. These supplements are available as individual D catalog numbers and as a kit D Resurge CD PK. D Resurge Media Supplement Product Name D Catalog # Quantity Unit Size D Resurge CD G KG KG D Resurge CD G KG KG D Resurge CD G KG KG 7 G D Resurge CD 7 7 KG KG D Resurge CD G KG KG D Resurge CD PK Product Name Quantity Unit Size D Resurge CD D Resurge CD G G D Catalog # D Resurge CD G 7 D Resurge CD G D Resurge CD G D, D Logo and all other trademarks are property of ecton, Dickinson and Company. D 8 June Printed in US D iosciences dvanced ioprocessing Tel bdbiosciences.com/advbio