BD MOSAIC h MSC CELL CULTURE ENVIRONMENT DEFINED SERUM FREE

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1 BD MOSAIC h MSC CELL CULTURE ENVIRONMENT DEFINED SERUM FREE

2 As the promise of stem cell therapy grows, so does the need for reliable tools for cell expansion in both research and clinical applications. BD Biosciences is at the forefront of development of next generation systems for the expansion of human Mesenchymal Stem Cells (hmsc) that provide researchers and clinicians consistency, performance, safety and lower costs in use. BD Mosaic hmsc SF is the first in a series of integrated cell culture environments for stem cell expansion. The product is a complete package that contains the media, supplement and surface required for cell expansion. PERFORMANCE. EFFICIENCY. SAFETY. Next generation cell culture environments from BD are developed to provide better performance over existing methods, simplify workflows, maintain multipotency, and reduce cost and risk across the continuum of research through clinical applications. BD Mosaic, for example, provides greater cell expansion in shorter culture time, higher cell doublings per passage (without re-feeding requirements), and lower minimum seeding densities as compared to traditional serum-containing methods, while maintaining normal MSC function. And because the system integrates media, supplement and surface, performance is further optimized at a dramatically lower cost in use. Next generation cell culture environments provide a complete package designed to increase the speed, consistency, and safety of cell expansion. DEFINED. SERUM FREE. Leveraging a half century worth of cell culture experience, next generation cell culture environments from BD Biosciences are developed through the rigorous application of bioinformatic and proprietary screening processes to improve the overall performance of next generation media, supplements and processes. The BD Mosaic hmsc Serum Free cell culture environment is produced using stringent sourcing and manufacturing controls to maximize safety, minimize potential cross contamination and provide performance with reliability and consistency. Using a defined medium, wherein every constituent is known and serum has been removed from the formulation, the BD Mosaic hmc SF cell culture environment provides a reliable, safe medium, supplement and surface coating for hmsc expansion. From isolation through production, BD Biosciences delivers high quality products and services, meeting your needs at every step in the cell-based workflow. As research moves toward clinical trials and ultimately new cell therapies, you can rely on BD s proven experience in delivering quality products and services as we deliver the next generation of tools and systems to help you transform disease management in the next decade. Systematic Excellence SM is our commitment to deliver both best-in-class and highly integrated products and services to help you reduce risk and to ensure superior quality in the production of clinical-grade therapies.

3 BD MOSAIC hmsc SF CELL CULTURE ENVIRONMENT. BD Mosaic Mesenchymal Stem Cell Serum-Free (hmsc SF) is a complete (media, supplement, and surface), defined cell culture environment for the expansion of human mesenchymal stem cells (hmscs) that can provide greater consistency than traditional serum-containing methods at a lower cost. It is the first product in BD's line of next-generation environments for the expansion of stem cells. The BD Mosaic hmsc cell culture environment is designed to provide cell expansion in a shorter culture time about half the time of serum-based media. Starting with a low minimum seeding density (3, 4, cells / cm 2 ), BD Mosaic can achieve 2 or more doublings per passage without re-feeding between passages. Such performance can shorten time to publication, lower total cost, and reduce handling and the risk of contamination. The performance provided by the BD Mosaic hmsc environment, coupled with its lot-to-lot consistency, can provide reliable results with speed and confidence. The serum-free composition lessens variability and potential contamination events to further streamline the cell culture process and reduce risk. SUPERIOR PERFORMANCE. Adopting the BD Mosaic hmsc SF cell culture environment can help reduce the risk, expense, and handling involved in culturing MSCs. BD Mosaic expands MSCs rapidly, while maintaining multipotency. There is no need to re-feed mid-passage. CELL EXPANSION VERSUS SERUM-CONTAINING MEDIUM SEEDING DENSITY HARVEST DENSITY PASSAGE FREQUENCY MEDIUM CHANGE FREQUENCY MEDIUM VOLUME NEEDED BD Mosaic hmsc SF Medium 3,-4, cells/cm 2 24, cells/cm 2 3 to 4 days None between passage.2 ml/cm 2 THEORETICAL SCALE-UP FROM A MILLION TO A BILLION CELLS MEDIUM REQUIRED (L) TOTAL SURFACE AREA (cmˆ2) TIME IN CULTURE (days) BD Mosaic hmsc SFM 17 5,25 2 Traditional (5% serum-containing medium) 5,, cells/cm 2 2, cells/cm 2 7 days 3 4 days.2.4 ml/cm 2-5% serum 2 24, 2 GREATER CELL EXPANSION IN SHORTER TIME. NO RE-FEEDING. BD Mosaic hmsc SF Competitor B (serum free) Cumulative Doublings with +/- 2SE 4 Cumulative Doublings with +/- 2SE 4 Cumulative Doublings with +/- 2SE DAYS DAYS DAYS Seeding Denisty 4, * 1, Cell Preperation 2 donors and 2 replicates Comparative population doublings of BD Mosaic vs. competition. In a comparative test against two commercially available media, BD Mosaic outperformed other systems, producing more cell doublings in less time.

4 RELIABLE MORPHOLOGY AND IMMUNOPHENOTYPE. BD Mosaic hmsc SF Competitor B (serum free) Cultured MSCs must maintain their morphology and immunophenotype if they are to have normal MSC characteristics and function. MSCs cultured with BD Mosaic exhibit a tight and compact morphology. MSCs cultured with BD Mosaic also maintain normal MSC immunophenotype as measured by flow cytometry for the ISCT SM surface markers. Cultured cells maintain a cell surface marker expression profile characteristic of MSCs. When compared to several other commercially available solutions, cells cultured with BD Mosaic were of higher quality using these measures. Cultured MSCs exhibit normal morphology MSCs were cultured either in BD Mosaic hmsc SF or with 2 other commercially available media and imaged. Results: Cells cultured in BD Mosaic were tight and compact, in comparison to other media tested. Passage 1 at Day 3 Passage 4 at Day 12 Passage 1 at Day 3 Passage 3 at Day 13 Passage 1 at Day 7 Passage 3 at Day % Positive Expression (+/- 2SE) CD CD CD Results: Compared with competitors after passage two and four virtually 1% of cultured MSCs in BD Mosaic displayed a cell surface market profile characteristic of MSCs with the expression of CD73, CD9 and CD15 and the absence of CD14, CD19 and CD79a. RELIABLE MULTILINEAGE POTENTIAL. BD Mosaic hmsc SF An intrinsic property of mesenchymal stem cells is their ability to differentiate into various somatic tissues (adipocytes, osteoblasts, chondrocytes). As with traditional methods, MSCs expanded using the BD Mosaic hmsc SF cell culture environment reliably retain their multipotency even after many passages. Uninduced BD Mosaic hmsc SF Induced BD Mosaic hmsc SF BD Mosaic hmsc SF Passage 2 Passage 4 CD14 CD34 CD45 CD79a HLA-DR CD73 CD9 CD15 +/- 2SE Cultured MSCs exhibit normal immunophenotype MSCs were cultured in BD Mosaic hmsc SF and separately with serum and then analyzed by flow cytometry for MSC surface markers. 12 TEST CD79a CD19 CD14 ISOTYPE Uninduced Passage 2 at Day Passage 4 at Day 12 Passage 3 at Day 13 OSTEOBLASTS Induced Cultured MSCs differentiate into adipocytes and osteoblasts MSCs were expanded in BD Mosaic hmsc SF and 5% serum medium and induced to differentiate into adipocytes or osteoblasts. Cells were stained with Hoechst (blue to identify nuclei) and either AdipoRed (to detect adipocytes) or alkaline phosphatase (to detect osteoblasts). Expanded but uninduced cells were also stained for comparison. Passage 2 at Day Passage 4 at Day 12 Passage 3 at Day 13 ADIPOCYTES Results: Staining showed that MSCs cultured for two or four passages in BD Mosaic were readily induced to differentiate into adipocytes or osteoblasts. Uninduced cells remained undifferentiated.

5 RELIABLE MULTILINEAGE POTENTIAL. (continued) 1x 2x Passage Uninduced Passage 2 Induced at Day 7 Passage 2 Induced BD Mosaic hmsc SF at Day Passage 5 Induced BD Mosaic hmsc SF at Day 17 CHONDROCYTES Maintenance of MSC multi-lineage potential to chondrocytes MSCs were expanded in BD Mosaic hmsc SF for two (center) or five (right) passages and induced to differentiate in a chondrogenic induction medium. Cells were stained with Alcian blue to detect chondrocytes. Unexpanded, uninduced cells (left) were also stained for comparison. Cells were imaged at 2x and 1x; mucins stained blue and nuclei stained red. Results: Staining showed that MSCs cultured for two or five passages in BD Mosaic were readily induced to differentiate into chondrocytes. Unexpanded, uninduced cells remained undifferentiated. RELIABLE T-CELL SUPPRESSION AT EARLY AND LATE PASSAGES. Another important measure of the cell quality is T-Cell suppression. MSCs cultured with BD Mosaic hmsc SF maintain their ability to suppress immune response as measured by T-cell proliferation x1 5 FITC BrdU Cultured MSCs suppress T-cell proliferation Activated T-cells (stimulated with anti-cd3/cd2) were cultured alone (blue) or co-cultured with expanded MSCs. After 4 days of co-culture, T-cells were stained with BrdU and analyzed for T-cell proliferation. MSCs were cultured in either BD Mosaic MSC SF medium or 5% serum medium for six () or eight (BD Mosaic hmsc SF) population doublings. Data for MSCs cultured in 5% serum are not plotted beyond doublings because MSCs could not be doubled more times in serum. Results: Plots of MSC-mediated T-cell proliferation (red, green) were more similar to unstimulated T-cells (negative controls, orange) than to activated T-cells (positive controls, blue). After eight doublings, MSCs cultured in BD Mosaic (red) suppressed 95% of T-cell proliferation, which was greater than MSCs cultured in serum (green, 2%). Unstimulated T-cells Activated T-cells Activated T-cells plus MSCs grown in (PD ) Activated T-cells plus MSCs grown in BD Mosaic hmsc SF (PD ) POPULATION DOUBLING (PD) GROWTH MEDIUM BD MOSAIC hmsc SF COMPETITOR A (5% SERUM) % SUPPRESSION 95 2

6 IMPLEMENTING BD MOSAIC. The BD Mosaic hmsc SF cell culture environment is designed to fit flexibly into your culture process. Developed for MSCs derived from bone marrow, it has also been effective in expanding MSCs from adipose and placental tissue. It is compatible with a broad range of cultureware including BD Falcon dishes and flasks. PRODUCED IN FDA-COMPLIANT FACILITY. The BD Mosaic hmsc SF cell culture environment is manufactured in a FDA registered facility following the Quality System Regulation standards and requirements in 21CFR2/ISO91. Additionally, the following regulations and guidance were used in the development of the product. (EC) No 1394/27 on advanced therapy medicinal products CBER Guidance For Industry: Guidance for Human Somatic Cell Therapy and Gene Therapy CBER Draft Guidance For Reviewers: Instructions and Template For Chemistry Manufacturing, and Control (CMC) Reviewers of Somatic Cell Therapy Investigational New Drug Applications (INDs) USP General Chapter 143 Ancillary Materials for Cell, Gene and Tissue Engineered Products BD Mosaic hmsc SF is a complete cell culture environment that includes medium, supplement, and surface. ORDERING INFORMATION Description Cat. No. Quantity BD Mosaic hmsc SF Cell Culture Environment kit Includes * : BD Mosaic hmsc SF Medium ml BD Mosaic hmsc SF Supplement ml BD Mosaic hmsc SF Surface Coating - not sold separately BD Accutase Cell Detachment Solution ml BD Stemflow hmsc Anaysis Kit tests Note: BD Mosaic is sold as a complete kit. For customized medium formulation and manufacturing requests, contact us at Answers@bd.com. For more information, visit bdbiosciences.com/mosaic, call BD Technical Service and Support at or , or contact your BD Biosciences Sales Associate. 5 mg 29 Concord Road Billerica, MA 121 USA tel: fax: Purchase Orders should be made out to: 235 Qume Drive, San Jose, CA BD 22 Argentia Road Mississauga, Ontario Canada L5N H tel: fax: BD Akasaka Garden City, Akasaka , Minato-ku, Tokyo, Japan tel: (1) fax: (1) Singapore Branch 3 Tuas Avenue 2 Singapore 3941 tel: (5) 1 33 fax: (5) 159 Erembodegem-Dorp 932 Erembodegem, Belgium tel: (32) fax: (32) contact_bdb@europe.bd.com 4 Research Park Drive Macquarie University Research Park North Ryde NSW 2113 Australia tel: fax: aus_customerservice@bd.com For Research Use Only. Not intended for use in diagnostic or therapeutic procedures. Accutase is a trademark of Innovation Cell Technologies, Inc. ISCT is a service mark of The International Society for Cellular Therapy. AdipoRed is a trademark of Lonza Group or its subsidiaries. BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. 212 BD. B11M11